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Dr. Gen Kaneko
University of Houston-Victoria

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0 Biochemistry
0 Evolution
0 Fish
0 Growth Hormone
0 Insulin

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Feed

Journal article
Published: 16 June 2021 in Animal Feed Science and Technology
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A 10-week feeding trial was conducted to examine the effect of dietary sacha inchi meal (SIM) as a soybean meal (SBM) replacer on growth performance, feed utilization, whole-body fatty acid composition, blood biochemistry, and histological changes in red hybrid tilapia, Oreochromis niloticus × O. mossambicus. Five isonitrogenous and isolipidic diets were formulated with 0 (control diet), 110, 220, 320, and 420 g/kg replacement of SBM with SIM (referred to as SIM0‒420). Each diet was randomly assigned to one of five fish groups in a 100 L indoor tank (n = 15 each, average initial weight 9.36 ± 0.02 g) with four replicates. During the last 2 weeks of the feeding trial, 5 g/kg chromic oxide (Cr2O3) was added to the diet to determine the apparent digestibility coefficient (ADC). The results showed that SIM inclusion significantly increased growth performance, feed utilization, and ADC of dry matter in a quadratic manner with the highest final weight observed in the SIM110 group. Survival rates were not significantly affected by SIM inclusion levels (P > 0.05). The amount of n-3 fatty acids in the whole-body fish, particularly that of C18:3n-3, was linearly and quadratically increased (P < 0.001) with increasing dietary SIM inclusions. Total cholesterol and triglycerides (TGs) were linearly and quadratically decreased in the fish fed the SIM inclusion diets (P < 0.05), but no linear or quadratic effects of dietary treatments were observed on levels of total protein, albumin, globulin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Liver histology showed a significant increase in cytoplasmic vacuolization with increasing SIM inclusion in diets, and fish fed the SIM420 diet showed hepatocyte swelling and displacement of hepatocyte nuclei toward the periphery. Similarly, intestine histology detected a significant linear reduction in villi height and villi thickness with increasing level of dietary SIM (P < 0.001). Overall, the present study indicates that SIM protein can totally replace soybean protein in tilapia diets, while high SIM inclusion results in histological abnormalities. In particular, SIM110 replacement can improve growth without any adverse effects in biochemical parameters, digestibility, and histological changes.

ACS Style

Anurak Khieokhajonkhet; Sutinun Muichanta; Niran Aeksiri; Khanitta Ruttarattanamongkol; Jiraporn Rojtinnakorn; Gen Kaneko. Evaluation of sacha inchi meal as a novel alternative plant protein ingredient for red hybrid tilapia (Oreochromis niloticus×O. mossambicus): Growth performance, feed utilization, blood biochemistry, and histological changes. Animal Feed Science and Technology 2021, 278, 115004 .

AMA Style

Anurak Khieokhajonkhet, Sutinun Muichanta, Niran Aeksiri, Khanitta Ruttarattanamongkol, Jiraporn Rojtinnakorn, Gen Kaneko. Evaluation of sacha inchi meal as a novel alternative plant protein ingredient for red hybrid tilapia (Oreochromis niloticus×O. mossambicus): Growth performance, feed utilization, blood biochemistry, and histological changes. Animal Feed Science and Technology. 2021; 278 ():115004.

Chicago/Turabian Style

Anurak Khieokhajonkhet; Sutinun Muichanta; Niran Aeksiri; Khanitta Ruttarattanamongkol; Jiraporn Rojtinnakorn; Gen Kaneko. 2021. "Evaluation of sacha inchi meal as a novel alternative plant protein ingredient for red hybrid tilapia (Oreochromis niloticus×O. mossambicus): Growth performance, feed utilization, blood biochemistry, and histological changes." Animal Feed Science and Technology 278, no. : 115004.

Review
Published: 12 May 2021 in Genes
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Precision medicine is a medical approach to administer patients with a tailored dose of treatment by taking into consideration a person’s variability in genes, environment, and lifestyles. The accumulation of omics big sequence data led to the development of various genetic databases on which clinical stratification of high-risk populations may be conducted. In addition, because cancers are generally caused by tumor-specific mutations, large-scale systematic identification of single nucleotide polymorphisms (SNPs) in various tumors has propelled significant progress of tailored treatments of tumors (i.e., precision oncology). Machine learning (ML), a subfield of artificial intelligence in which computers learn through experience, has a great potential to be used in precision oncology chiefly to help physicians make diagnostic decisions based on tumor images. A promising venue of ML in precision oncology is the integration of all available data from images to multi-omics big data for the holistic care of patients and high-risk healthy subjects. In this review, we provide a focused overview of precision oncology and ML with attention to breast cancer and glioma as well as the Bayesian networks that have the flexibility and the ability to work with incomplete information. We also introduce some state-of-the-art attempts to use and incorporate ML and genetic information in precision oncology.

ACS Style

Mahaly Baptiste; Sarah Moinuddeen; Courtney Soliz; Hashimul Ehsan; Gen Kaneko. Making Sense of Genetic Information: The Promising Evolution of Clinical Stratification and Precision Oncology Using Machine Learning. Genes 2021, 12, 722 .

AMA Style

Mahaly Baptiste, Sarah Moinuddeen, Courtney Soliz, Hashimul Ehsan, Gen Kaneko. Making Sense of Genetic Information: The Promising Evolution of Clinical Stratification and Precision Oncology Using Machine Learning. Genes. 2021; 12 (5):722.

Chicago/Turabian Style

Mahaly Baptiste; Sarah Moinuddeen; Courtney Soliz; Hashimul Ehsan; Gen Kaneko. 2021. "Making Sense of Genetic Information: The Promising Evolution of Clinical Stratification and Precision Oncology Using Machine Learning." Genes 12, no. 5: 722.

Journal article
Published: 02 December 2020 in Gene
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The fish insulin/insulin-like growth factor (IGF) pathway has weak control over carbohydrate metabolism. To understand the molecular basis for the metabolic diversity, we characterized the forkhead box transcription factor O1A (FoxO1A), a downstream target of the insulin/IGF pathway, in torafugu Takifugu rubripes. The cloned torafugu FoxO1A cDNA contained all conserved features critical for its transcriptional activity and a unique unspliced intron encoding a poly-glutamine stretch. Torafugu FoxO1A showed the IGF-dependent nuclear exclusion and in vitro binding to the well-conserved FoxO1 binding site, DAF-16-binding element (DBE), but failed to bind to the insulin-responsive element by which mammalian FoxO1 mediates insulin effects. The subsequent in silico genomic screening provided a list of 587 potential torafugu FoxO1A target genes containing the DBE. Some carbohydrate metabolic genes regulated by FoxO1 in mammals were not included in the list. We further identified about 250 potential fish FoxO1 target genes by integrating results of the DBE screening against fish metagenome that contained 262 species. Neuronal processes appeared to be the common major function of fish FoxO1, although further annotation of the potential target genes is required. These results provide a part of the molecular basis underlying the weak association between the insulin/IGF pathway and carbohydrate metabolism in fish.

ACS Style

Anthony Canela Reyes; Elvis Egwu; Ermeng Yu; Ashley N. Sanchez; Linda De La O; Osasenaga Emmanuel Elijah; Tyler J Muschalek; Wei Zhang; Hong Ji; Hashimul Ehsan; Gen Kaneko. Forkhead transcription factor O1 (FoxO1) in torafugu pufferfish Takifugu rubripes: Molecular cloning, in vitro DNA binding, and target gene screening in fish metagenome. Gene 2020, 768, 145335 .

AMA Style

Anthony Canela Reyes, Elvis Egwu, Ermeng Yu, Ashley N. Sanchez, Linda De La O, Osasenaga Emmanuel Elijah, Tyler J Muschalek, Wei Zhang, Hong Ji, Hashimul Ehsan, Gen Kaneko. Forkhead transcription factor O1 (FoxO1) in torafugu pufferfish Takifugu rubripes: Molecular cloning, in vitro DNA binding, and target gene screening in fish metagenome. Gene. 2020; 768 ():145335.

Chicago/Turabian Style

Anthony Canela Reyes; Elvis Egwu; Ermeng Yu; Ashley N. Sanchez; Linda De La O; Osasenaga Emmanuel Elijah; Tyler J Muschalek; Wei Zhang; Hong Ji; Hashimul Ehsan; Gen Kaneko. 2020. "Forkhead transcription factor O1 (FoxO1) in torafugu pufferfish Takifugu rubripes: Molecular cloning, in vitro DNA binding, and target gene screening in fish metagenome." Gene 768, no. : 145335.

Journal article
Published: 06 November 2020 in Foods
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Crisp grass carp products from China are becoming more prevalent in the worldwide fish market because muscle hardness is the primary desirable characteristic for consumer satisfaction of fish fillet products. Unfortunately, current instrumental methods to evaluate muscle hardness are expensive, time-consuming, and wasteful. This study sought to develop classification models for differentiating the muscle hardness of crisp grass carp on the basis of blood analysis. Out of the total 264 grass carp samples, 12 outliers from crisp grass carp group were removed based on muscle hardness (2O2), glucose 6-phosphate dehydrogenase (G6PD), malondialdehyde (MDA), glutathione (GSH/GSSH), red blood cells (RBC), platelet count (PLT), and lymphocytes (LY). Furthermore, six machine learning models were applied to predict the muscle hardness of grass carp based on the training (152) and testing (100) datasets obtained from the blood analysis: random forest (RF), naïve Bayes (NB), gradient boosting decision tree (GBDT), support vector machine (SVM), partial least squares regression (PLSR), and artificial neural network (ANN). The RF model exhibited the best prediction performance with a classification accuracy of 100%, specificity of 93.08%, and sensitivity of 100% for discriminating crisp grass carp muscle hardness, followed by the NB model (93.75% accuracy, 91.83% specificity, and 94% sensitivity), whereas the ANN model had the lowest prediction performance (85.42% accuracy, 81.05% specificity, and 85% sensitivity). These machine learning methods provided objective, cheap, fast, and reliable classification for in vivo crisp grass carp and also prove useful for muscle quality evaluation of other freshwater fish.

ACS Style

Bing Fu; Gen Kaneko; Jun Xie; Zhifei Li; Jingjing Tian; Wangbao Gong; Kai Zhang; Yun Xia; Ermeng Yu; Guangjun Wang. Value-Added Carp Products: Multi-Class Evaluation of Crisp Grass Carp by Machine Learning-Based Analysis of Blood Indexes. Foods 2020, 9, 1615 .

AMA Style

Bing Fu, Gen Kaneko, Jun Xie, Zhifei Li, Jingjing Tian, Wangbao Gong, Kai Zhang, Yun Xia, Ermeng Yu, Guangjun Wang. Value-Added Carp Products: Multi-Class Evaluation of Crisp Grass Carp by Machine Learning-Based Analysis of Blood Indexes. Foods. 2020; 9 (11):1615.

Chicago/Turabian Style

Bing Fu; Gen Kaneko; Jun Xie; Zhifei Li; Jingjing Tian; Wangbao Gong; Kai Zhang; Yun Xia; Ermeng Yu; Guangjun Wang. 2020. "Value-Added Carp Products: Multi-Class Evaluation of Crisp Grass Carp by Machine Learning-Based Analysis of Blood Indexes." Foods 9, no. 11: 1615.

Article
Published: 21 September 2020
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The metazoan 70-kDa heat shock protein (HSP70) family contains several members localized in different subcellular compartments. The cytosolic members have been classified into inducible HSP70s and constitutive heat shock cognates (HSC70s), but their distinction and evolutionary relationship remain unclear because of occasional reports of “constitutive HSP70s” and the lack of cross-phylum comparisons. Here we provide novel insights into the evolution of these important molecular chaperones. Phylogenetic analyses of ∼100 full-length HSP70s revealed an ancient duplication that gave rise to two lineages from which all metazoan cytosolic HSP70s descend. One lineage (A) contains a relatively small number of Lophotrochozoan and Ecdysozoan genes, none of which have been shown to be constitutively expressed (i.e., either inducible or unknown). The other lineage (B) included both inducible and constitutive genes from diverse phyla. Species-specific duplications are present in both lineages, and Lineage B contains well-supported phylum-specific clades for Rotifera, Nematoda, and Chordata. Some genes in Lineage B have likely independently acquired inducibility, which may explain the sporadic distribution of “HSP70” or “HSC70” in previous analyses. Consistent with the diversification history within each group, inducible members show lower purifying selection pressure compared to constitutive members. These results illustrate the evolutionary history of the HSP70 family, encouraging us to propose a new nomenclature: “HSP70 + subcellular localization + linage + copy number in the organism + inducible or constitutive, if known.” e.g., HSP70cA1i for cytosolic Lineage A, copy 1, inducible.

ACS Style

Er-Meng Yu; Tatsuki Yoshinaga; Frank L. Jalufka; Hashimul Ehsan; David B. Mark Welch; Gen Kaneko. The complex evolution of the metazoan HSP70 gene family. 2020, 1 .

AMA Style

Er-Meng Yu, Tatsuki Yoshinaga, Frank L. Jalufka, Hashimul Ehsan, David B. Mark Welch, Gen Kaneko. The complex evolution of the metazoan HSP70 gene family. . 2020; ():1.

Chicago/Turabian Style

Er-Meng Yu; Tatsuki Yoshinaga; Frank L. Jalufka; Hashimul Ehsan; David B. Mark Welch; Gen Kaneko. 2020. "The complex evolution of the metazoan HSP70 gene family." , no. : 1.

Letter to the editor
Published: 10 August 2020 in Journal of Medical Virology
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Several recent studies have reported that systemic corticosteroids are effective against COVID‐191. COVID‐19 mainly affects the respiratory system, with minor damage to other organs. This article is protected by copyright. All rights reserved.

ACS Style

Daisuke Miyazawa; Gen Kaneko. Clinical trials of inhaled beclomethasone and mometasone for COVID‐19 should be conducted. Journal of Medical Virology 2020, 93, 637 -638.

AMA Style

Daisuke Miyazawa, Gen Kaneko. Clinical trials of inhaled beclomethasone and mometasone for COVID‐19 should be conducted. Journal of Medical Virology. 2020; 93 (2):637-638.

Chicago/Turabian Style

Daisuke Miyazawa; Gen Kaneko. 2020. "Clinical trials of inhaled beclomethasone and mometasone for COVID‐19 should be conducted." Journal of Medical Virology 93, no. 2: 637-638.

Journal article
Published: 07 July 2020 in PeerJ
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Faba bean (Vicia faba, FB) is known to improve the texture of fish meat but retards growth possibly by inducing hemolysis, hepatopancreas damage, and metabolic disorder. In this study, we used ultrasonic processing to isolate four FB extracts (water extract, alcohol extract, proteins and residues) and examined their beneficial and detrimental effects. These extracts were separately mixed with commercial feed and fed to grass carp (Ctenopharyngodon idellus) using whole FB and commercial feed as controls. After fish were fed one of the six experimental diets for 50 d and 100 d, we evaluated the growth and hematological parameters, activities of metabolic enzymes, hepatopancreatic histology and oxidative response, and lipid metabolism. Results showed that both whole FB and FB residues caused growth retardation and hepatopancreas damage (P < 0.05), whereas growth performance was improved in the FB water and alcohol extract groups compared to the whole FB group. Although the FB water extract negatively affected the number and morphological parameters of red blood cells (P < 0.05), the hematological damage was less pronounced than that of the whole FB group. Excessive hepatopancreatic fat accumulation was found in the whole FB, FB alcohol extract and FB residues groups. Moreover, serious hepatopancreas damages were observed in the FB residues group. These results suggest that the beneficial and detrimental components of FB were successfully separated in the four extracts, and the FB water extract would be the best choice for grass carp culture in terms of growth performance and health. The safety evaluation of the four FB extracts would facilitate further application of FB in aquatic feed.

ACS Style

Lingling Ma; Gen Kaneko; Jun Xie; Guangjun Wang; Zhifei Li; Jingjing Tian; Kai Zhang; Yun Xia; Wangbao Gong; Haihang Li; Ermeng Yu. Safety evaluation of four faba bean extracts used as dietary supplements in grass carp culture based on hematological indices, hepatopancreatic function and nutritional condition. PeerJ 2020, 8, e9516 .

AMA Style

Lingling Ma, Gen Kaneko, Jun Xie, Guangjun Wang, Zhifei Li, Jingjing Tian, Kai Zhang, Yun Xia, Wangbao Gong, Haihang Li, Ermeng Yu. Safety evaluation of four faba bean extracts used as dietary supplements in grass carp culture based on hematological indices, hepatopancreatic function and nutritional condition. PeerJ. 2020; 8 ():e9516.

Chicago/Turabian Style

Lingling Ma; Gen Kaneko; Jun Xie; Guangjun Wang; Zhifei Li; Jingjing Tian; Kai Zhang; Yun Xia; Wangbao Gong; Haihang Li; Ermeng Yu. 2020. "Safety evaluation of four faba bean extracts used as dietary supplements in grass carp culture based on hematological indices, hepatopancreatic function and nutritional condition." PeerJ 8, no. : e9516.

Other
Published: 23 June 2020
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Identifying biomedical and socioeconomic predictors of the number of deaths caused by COVID-19 can help the development of effective interventions. In this study, we used the hypothesis-driven regression approach to test the hypothesis that the mask wearing rate, along with age and obesity, can largely predict the cumulative number of deaths across countries. Our regression models explained 69% of the variation in the cumulative number of deaths per million (March to June 2020) among 22 countries, identifying the face mask wearing rate in March as an important predictor. The number of deaths per million predicted by our elastic net regression model showed high correlation (r = 0.86) with observed numbers. These findings emphasize the importance of face masks in preventing the ongoing pandemic of COVID-19.One Sentence SummaryFace mask wearing rate in March is a strong predictor of the cumulative number of deaths per million caused by COVID-19 among 22 countries.

ACS Style

Daisuke Miyazawa; Gen Kaneko. Face mask wearing rate predicts COVID-19 death rates across countries. 2020, 1 .

AMA Style

Daisuke Miyazawa, Gen Kaneko. Face mask wearing rate predicts COVID-19 death rates across countries. . 2020; ():1.

Chicago/Turabian Style

Daisuke Miyazawa; Gen Kaneko. 2020. "Face mask wearing rate predicts COVID-19 death rates across countries." , no. : 1.

Journal article
Published: 24 April 2020 in Food Chemistry
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Reactive oxygen species (ROS) regulate metabolism and chemical composition of various tissues. To understand how ROS affect the textural quality of fish muscle, we performed a multi-omics analysis on an established crisp grass carp model fed with a natural pro-oxidant faba bean. ROS levels were systemically and significantly increased up to three-fold in crisp grass carp, improving the muscle texture. Lipid metabolism was significantly enhanced up to five-fold in muscle and liver possibly to compensate the impaired carbohydrate metabolism of these tissues, but this caused further local ROS production. Mitochondrial damage associated with autophagy was evident in crisp grass carp. Proteomics revealed that elevated ROS likely disturbed the actin-myosin interaction and collagen turnover inducing fragmentation of myofibrillar proteins, all of which could have positively impacted the textural quality. The systemic metabolic changes that lead to the partial collapse of redox regulation likely underlie the ROS-induced improvement of textural quality.

ACS Style

Ermeng Yu; Bing Fu; Guangjun Wang; Zhifei Li; Dewei Ye; Yong Jiang; Hong Ji; Xia Wang; Deguang Yu; Hashimul Ehsan; Wangbao Gong; Kai Zhang; Jingjing Tian; Lingyun Yu; Zhiyi Hu; Jun Xie; Gen Kaneko. Proteomic and metabolomic basis for improved textural quality in crisp grass carp (Ctenopharyngodon idellus C.et V) fed with a natural dietary pro-oxidant. Food Chemistry 2020, 325, 126906 .

AMA Style

Ermeng Yu, Bing Fu, Guangjun Wang, Zhifei Li, Dewei Ye, Yong Jiang, Hong Ji, Xia Wang, Deguang Yu, Hashimul Ehsan, Wangbao Gong, Kai Zhang, Jingjing Tian, Lingyun Yu, Zhiyi Hu, Jun Xie, Gen Kaneko. Proteomic and metabolomic basis for improved textural quality in crisp grass carp (Ctenopharyngodon idellus C.et V) fed with a natural dietary pro-oxidant. Food Chemistry. 2020; 325 ():126906.

Chicago/Turabian Style

Ermeng Yu; Bing Fu; Guangjun Wang; Zhifei Li; Dewei Ye; Yong Jiang; Hong Ji; Xia Wang; Deguang Yu; Hashimul Ehsan; Wangbao Gong; Kai Zhang; Jingjing Tian; Lingyun Yu; Zhiyi Hu; Jun Xie; Gen Kaneko. 2020. "Proteomic and metabolomic basis for improved textural quality in crisp grass carp (Ctenopharyngodon idellus C.et V) fed with a natural dietary pro-oxidant." Food Chemistry 325, no. : 126906.

Journal article
Published: 01 January 2020 in Food Chemistry
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Fish muscle firmness is an important quality trait for consumer acceptance. Phosphorylation is known to change chemical and physical properties of proteins and is thus expected to affect muscle firmness, but only few such phosphoproteins have been identified. To explore phosphoproteins that affect fish muscle firmness, firm muscle (crisp grass carp) and soft muscle (ordinary grass carp) were analyzed by quantitative phosphoproteomics. We identified 27 up-regulated and 22 down-regulated phosphopeptides in crisp grass carp (ratio ≥1.5 or ≤0.667, and P-value < 0.05) and their potential upstream kinases. Protein-protein interaction analysis clustered these phosphoproteins into four groups, many of which have been suggested to impact muscle firmness and its postmortem changes: muscle fiber, connective tissue, carbohydrate metabolism and signal regulation. These results provide novel insights into the role of protein phosphorylation in fish muscle firmness and will contribute to the quality improvement of fish products.

ACS Style

Lunjian Chen; Jie Liu; Gen Kaneko; Jun Xie; Guangjun Wang; Deguang Yu; Zhifei Li; Lingling Ma; Da Qi; Jingjing Tian; Wangbao Gong; Kai Zhang; Ermeng Yu. Quantitative phosphoproteomic analysis of soft and firm grass carp muscle. Food Chemistry 2020, 303, 125367 .

AMA Style

Lunjian Chen, Jie Liu, Gen Kaneko, Jun Xie, Guangjun Wang, Deguang Yu, Zhifei Li, Lingling Ma, Da Qi, Jingjing Tian, Wangbao Gong, Kai Zhang, Ermeng Yu. Quantitative phosphoproteomic analysis of soft and firm grass carp muscle. Food Chemistry. 2020; 303 ():125367.

Chicago/Turabian Style

Lunjian Chen; Jie Liu; Gen Kaneko; Jun Xie; Guangjun Wang; Deguang Yu; Zhifei Li; Lingling Ma; Da Qi; Jingjing Tian; Wangbao Gong; Kai Zhang; Ermeng Yu. 2020. "Quantitative phosphoproteomic analysis of soft and firm grass carp muscle." Food Chemistry 303, no. : 125367.

Journal article
Published: 23 October 2019 in Aquaculture
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Faba bean (Vicia faba) is proven to improve muscle textural quality in fish, but also causes growth retardation possibly inducing oxidative damage and/or nutritional disturbances. Here we used ultrasonic processing to isolate four faba bean extracts (FB water extract, alcohol extract, proteins, and residues), and then these extracts were mixed with commercial feed. Whole faba bean and commercial feed were used as controls. After grass carp (Ctenopharyngodon idellus) were fed with the six experimental diets for 100 d, their growth, textural quality, oxidative response, and gut characteristics were analyzed. Results showed that whole faba bean and FB water extract significantly increased textural parameters (hardness, springiness, and chewiness), collagen content, and fiber density in grass carp muscle (P < 0.05). Also, the whole faba bean and FB water extract contained similar contents of vicine, a pro-oxidant that has been supposed to improve the textural quality, suggesting that vicine may be responsible for the improvement of textural quality in grass carp. However, importantly, vicine was not likely to be the growth inhibitor in faba bean because the growth rate of the FB water extract group was much higher than that of the whole faba beans group (P < 0.05). The presence of other oxidative substance(s) and anti-nutritional factors was suggested because both FB water and alcohol extract groups showed reduced intestinal oxidative damage and improved hematological indexes compared with the whole faba beans group (P < 0.05); indeed, FB residues group showed the worst oxidative responses and the lowest growth rate among all group tested. Taken together, this study highlights the potential usefulness of FB water extract in aquaculture and warrants further identification of functionally active component of faba bean.

ACS Style

Ling-Ling Ma; Gen Kaneko; Xiu-Juan Wang; Jun Xie; Jing-Jing Tian; Kai Zhang; Guang-Jun Wang; De-Guang Yu; Zhi-Fei Li; Wang-Bao Gong; Er-Meng Yu; Hai-Hang Li. Effects of four faba bean extracts on growth parameters, textural quality, oxidative responses, and gut characteristics in grass carp. Aquaculture 2019, 516, 734620 .

AMA Style

Ling-Ling Ma, Gen Kaneko, Xiu-Juan Wang, Jun Xie, Jing-Jing Tian, Kai Zhang, Guang-Jun Wang, De-Guang Yu, Zhi-Fei Li, Wang-Bao Gong, Er-Meng Yu, Hai-Hang Li. Effects of four faba bean extracts on growth parameters, textural quality, oxidative responses, and gut characteristics in grass carp. Aquaculture. 2019; 516 ():734620.

Chicago/Turabian Style

Ling-Ling Ma; Gen Kaneko; Xiu-Juan Wang; Jun Xie; Jing-Jing Tian; Kai Zhang; Guang-Jun Wang; De-Guang Yu; Zhi-Fei Li; Wang-Bao Gong; Er-Meng Yu; Hai-Hang Li. 2019. "Effects of four faba bean extracts on growth parameters, textural quality, oxidative responses, and gut characteristics in grass carp." Aquaculture 516, no. : 734620.

Journal article
Published: 08 August 2019 in Journal of Morphological Sciences
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The spotted bent-toed gecko Cyrtodactylus peguensis is one of the exploited reptiles in Thailand. In order to provide basic information for the digestive system of this species, we have examined histologically the gastrointestinal and accessory organs of C. peguensis using routine methods. The gastrointestinal region of this reptile started from the stomach and the intestine. The stomach was separated into fundic and pyloric regions. In both regions, the stomach wall was formed by four distinct tissue layers, including mucosa, submucosa, muscularis, and serosa layers. Mucous neck cells and oxynticopeptic cells were identified as glycoprotein-producing cells in the stomach by Periodic acid-Schiff (PAS) staining. The small and large intestines shared many histological characteristics, but the former contained more intestinal folds, while the latter had more PAS-positive goblet cells. Histological characteristics of accessory organs, liver and pancreas, were also provided. Overall, the gastrointestinal and accessory organs of C. peguensis were largely similar to those from other reptiles, but fine structural information will open up considerable opportunities to further studies related to the endocrinology, the physiology, and the conservation of this species.

ACS Style

Lamai Thongboon; Sinlapachai Senarat; Jes Kettratad; Wannee Jiraungkoorskul; Sansareeya Wangkulangkul; Pisit Poolprasert; Chamnan Para; Gen Kaneko; Theerakamol Pengsaku. Gastrointestinal Tract and Accessory Organs in the Spotted Bent-toed Gecko, Cyrtodactylus peguensis (Boulenger, 1893): A Histological and Histochemical Study. Journal of Morphological Sciences 2019, 36, 223 -230.

AMA Style

Lamai Thongboon, Sinlapachai Senarat, Jes Kettratad, Wannee Jiraungkoorskul, Sansareeya Wangkulangkul, Pisit Poolprasert, Chamnan Para, Gen Kaneko, Theerakamol Pengsaku. Gastrointestinal Tract and Accessory Organs in the Spotted Bent-toed Gecko, Cyrtodactylus peguensis (Boulenger, 1893): A Histological and Histochemical Study. Journal of Morphological Sciences. 2019; 36 (04):223-230.

Chicago/Turabian Style

Lamai Thongboon; Sinlapachai Senarat; Jes Kettratad; Wannee Jiraungkoorskul; Sansareeya Wangkulangkul; Pisit Poolprasert; Chamnan Para; Gen Kaneko; Theerakamol Pengsaku. 2019. "Gastrointestinal Tract and Accessory Organs in the Spotted Bent-toed Gecko, Cyrtodactylus peguensis (Boulenger, 1893): A Histological and Histochemical Study." Journal of Morphological Sciences 36, no. 04: 223-230.

Journal article
Published: 01 August 2019 in Asia Pacific Journal of Molecular Biology and Biotechnology
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The reproductive physiology concerning the gonadotropin hormone (GTH) and its downstream target estrogen receptor alpha (ERα) is not well understood in the short mackerel Rastrelliger brachysoma, an economically important marine fish in Thailand. In this study, we tested the hypothesis that the expression of both GTH and ERα in the brain and ovary of R. brachysoma was as reported in other fish species. By applying immunohistochemical techniques, we identified the distribution of ERα-immunoreactive (ir) neurons in the brain and ovary of wild female R. brachysoma during the spawning season along with the distribution of GTHs-ir cells in the ovary. The nucleus lateralis tuberis in the diencephalon had a high number of ERα-ir neurons. In the mesencephalon, dense ERα-ir neuronal fibers were mainly found in the mesencephalic cells, stratum opticum, stratum fibrosum et griseum superficiale and stratum album centrale. Both the valvula and corpus cerebelli in the metencephalon contained ERα-ir neurons in granular and Purkinje cell layers as well as the molecular layer. The ERα-ir neurons were also observed in the medulla oblongata. In the ovary, weak ERα and moderate GTHs immunoreactivities were observed in follicular cells of oocytes in early and late vitellogenic stages. This information provides baseline data required to understand not only the activity of estrogen (E2) on the brain but also the regulatory mechanism of the hypothalamo-pituitary-ovarian axis of R. brachysoma.

ACS Style

Sinlapachai Senarat; Jes Kettratad; Niwat Kangwanrangsan; Wannee Jiraungkoorskul; Francis Gerald Plumley; Masafumi Amano; Akio Shimizu; Piyakorn Boonyoung; Gen Kaneko. Immunoreactivity of estrogen receptor alpha in brain and ovary of the short mackerel Rastrelliger brachysoma (Bleeker, 1851). Asia Pacific Journal of Molecular Biology and Biotechnology 2019, 50 -63.

AMA Style

Sinlapachai Senarat, Jes Kettratad, Niwat Kangwanrangsan, Wannee Jiraungkoorskul, Francis Gerald Plumley, Masafumi Amano, Akio Shimizu, Piyakorn Boonyoung, Gen Kaneko. Immunoreactivity of estrogen receptor alpha in brain and ovary of the short mackerel Rastrelliger brachysoma (Bleeker, 1851). Asia Pacific Journal of Molecular Biology and Biotechnology. 2019; ():50-63.

Chicago/Turabian Style

Sinlapachai Senarat; Jes Kettratad; Niwat Kangwanrangsan; Wannee Jiraungkoorskul; Francis Gerald Plumley; Masafumi Amano; Akio Shimizu; Piyakorn Boonyoung; Gen Kaneko. 2019. "Immunoreactivity of estrogen receptor alpha in brain and ovary of the short mackerel Rastrelliger brachysoma (Bleeker, 1851)." Asia Pacific Journal of Molecular Biology and Biotechnology , no. : 50-63.

Article
Published: 26 February 2019 in Fish Physiology and Biochemistry
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Liver X receptor (LXR) is a ligand-activated transcription factor that plays vital roles in maintaining cholesterol and lipid homeostasis. Much work has been done on mammalian LXRs, but the role of LXR in fish remains unclear. In the present study, LXR gene was identified from adult Asian seabass, Lates calcarifer, and its predicted protein structure was docked with several cholesterol derivatives at the binding site. The LXR cDNA consisted of 1495 bp encoding a putative LXR protein of 494 amino acids. The Asian seabass LXR retained many important structural features found in LXRs of other fishes and mammals, such as putative signal peptide, activation function-1 (AF-1) domain, DNA-binding domain (DBD), ligand-binding domain (LBD), activation function-2 (AF-2) domain, and eight conserved cysteine residues. The deduced amino acid sequence of LXR shared significant identity with those of other species ranging from 65.7 to 95.8%. The homology modeling and in silico molecular docking demonstrated that Asian seabass LXR could interact with cholesterol derivatives at amino acid residues Phe274 and Ile312. Real-time PCR further revealed that LXR transcripts are ubiquitously expressed in all tissues examined, with the highest levels detected in the gonad followed by the liver. Given the well-known importance of cholesterol-mediated signaling in these tissues, Asian seabass LXR may reasonably be involved in reproduction and lipid metabolism.

ACS Style

Anurak Khieokhajonkhet; Niran Aeksiri; Gen Kaneko. Molecular characterization and homology modeling of liver X receptor in Asian seabass, Lates calcarifer: predicted functions in reproduction and lipid metabolism. Fish Physiology and Biochemistry 2019, 45, 523 -538.

AMA Style

Anurak Khieokhajonkhet, Niran Aeksiri, Gen Kaneko. Molecular characterization and homology modeling of liver X receptor in Asian seabass, Lates calcarifer: predicted functions in reproduction and lipid metabolism. Fish Physiology and Biochemistry. 2019; 45 (2):523-538.

Chicago/Turabian Style

Anurak Khieokhajonkhet; Niran Aeksiri; Gen Kaneko. 2019. "Molecular characterization and homology modeling of liver X receptor in Asian seabass, Lates calcarifer: predicted functions in reproduction and lipid metabolism." Fish Physiology and Biochemistry 45, no. 2: 523-538.

Original article
Published: 20 February 2019 in Aquaculture Research
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Lipid distribution pattern is an important trait, which is related to flesh quality and the yield rate of aquaculture products. Here we investigated lipid distribution patterns by oil red O staining for nine economically important fish in Thailand. The most remarkable feature was that Siluriformes species had a thick layer of subcutaneous lipids, possibly to compensate the evolutionary loss of scales. In addition, we found several other characteristic staining patterns: (1) dotted stains at the boundary of red and white muscles and along the subcutaneous tissue in crystal‐eyed catfish, (2) double lines of staining along the subcutaneous layer in crystal‐eyed catfish, (3) dotted stains along the horizontal septum lines in hybrid catfish, (4) pronounced retroperitoneal fat accumulation in Nile tilapia and red tilapia, (5) pronounced fat accumulation in the inclinator muscle of the fins in Indian spiny turbot, (6) intense staining under the scales of common carp and (7) three dotted stains in soft finrays in clown knifefish. Although lipid distribution patterns are known to be affected by environmental conditions, the observed species differences suggest the strong genetic control of lipid distribution. This trait should be considered as a target of selective breeding especially for Siluriformes, in addition to commonly used parameters such as growth rate and disease resistance.

ACS Style

Anurak Khieokhajonkhet; Sunisa Klongchai; Orrawan Maphum; Gen Kaneko. Lipid distribution patterns of nine commercial fish in Thailand. Aquaculture Research 2019, 50, 1348 -1360.

AMA Style

Anurak Khieokhajonkhet, Sunisa Klongchai, Orrawan Maphum, Gen Kaneko. Lipid distribution patterns of nine commercial fish in Thailand. Aquaculture Research. 2019; 50 (4):1348-1360.

Chicago/Turabian Style

Anurak Khieokhajonkhet; Sunisa Klongchai; Orrawan Maphum; Gen Kaneko. 2019. "Lipid distribution patterns of nine commercial fish in Thailand." Aquaculture Research 50, no. 4: 1348-1360.

Review article
Published: 09 November 2018 in Fisheries Science
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Magnetic resonance technologies, such as nuclear magnetic resonance (NMR) spectroscopy and magnetic resonance imaging (MRI), are powerful tools used in various fields of science. In this review, we provide a broad overview of the application of magnetic resonance technologies in aquatic biology following brief descriptions of their principles and characteristics. The topics covered include in vitro and in vivo NMR spectroscopy, NMR metabolomics, MRI, and their application to seafood science. Special attention is paid to in vivo NMR spectroscopy and metabolic tracing using a stable isotope, 13C, which provide safe and effective means of exploring the metabolic diversity of aquatic organisms.

ACS Style

Gen Kaneko; Hideki Ushio; Hong Ji. Application of magnetic resonance technologies in aquatic biology and seafood science. Fisheries Science 2018, 85, 1 -17.

AMA Style

Gen Kaneko, Hideki Ushio, Hong Ji. Application of magnetic resonance technologies in aquatic biology and seafood science. Fisheries Science. 2018; 85 (1):1-17.

Chicago/Turabian Style

Gen Kaneko; Hideki Ushio; Hong Ji. 2018. "Application of magnetic resonance technologies in aquatic biology and seafood science." Fisheries Science 85, no. 1: 1-17.

Journal article
Published: 26 October 2018 in Gene
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Smad4 is the key regulator in the transforming growth factor β1 (TGF-β1)/Smads signal pathway, and is also the crux of the regulation of type I collagen expression in mammals. In fish, however, the relationship between Smad4 and type I collagen is still unknown. Given the widely accepted importance of type I collagen in fish muscle hardness, we seek to explore this issue by analyzing the expressions of the TGF-β1/Smads pathway molecules and type I collagen in the muscle of crisp grass carp fed with faba bean, which shows increased muscle hardness. The study found that (1) in the process of feeding the grass carp with faba bean, the mRNA and protein expressions of TGF-β1, Smad2 and Smad4 all increased along with the increase of type I collagen expression (Col1α1 and Col1α2); (2) one day after the injection of Smad4 over-expression vector, both mRNA and protein expressions of Col1α1 and Col1α2 significantly increased, reaching the maximum on the 2nd and 5th day, respectively; (3) one day after the injection of Smad4 RNAi interference vector, the mRNA and protein expressions of Col1α1 and Col1α2 decreased, reaching the minimum on the 5th day. These results revealed that Smad4 is the major regulator of type I collagen in the muscle of grass carp fed with faba bean. This study would provide an important mechanistic basis for nutritional regulation of type I collagen in the muscle of fish.

ACS Style

Er-Meng Yu; Ling-Ling Ma; Hong Ji; Zhi-Fei Li; Guang-Jun Wang; Jun Xie; De-Guang Yu; Gen Kaneko; Jing-Jing Tian; Kai Zhang; Wang-Bao Gong. Smad4-dependent regulation of type I collagen expression in the muscle of grass carp fed with faba bean. Gene 2018, 685, 32 -41.

AMA Style

Er-Meng Yu, Ling-Ling Ma, Hong Ji, Zhi-Fei Li, Guang-Jun Wang, Jun Xie, De-Guang Yu, Gen Kaneko, Jing-Jing Tian, Kai Zhang, Wang-Bao Gong. Smad4-dependent regulation of type I collagen expression in the muscle of grass carp fed with faba bean. Gene. 2018; 685 ():32-41.

Chicago/Turabian Style

Er-Meng Yu; Ling-Ling Ma; Hong Ji; Zhi-Fei Li; Guang-Jun Wang; Jun Xie; De-Guang Yu; Gen Kaneko; Jing-Jing Tian; Kai Zhang; Wang-Bao Gong. 2018. "Smad4-dependent regulation of type I collagen expression in the muscle of grass carp fed with faba bean." Gene 685, no. : 32-41.

Rotifera xv
Published: 24 October 2018 in Hydrobiologia
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Despite the increasing environmental concern about the emission of ethanol from ethanol-blended fuels, the aquatic toxicity of ethanol has not been well investigated especially at low concentrations. In this study, we aimed to investigate the effects of 0.1% ethanol on life history parameters and population dynamics of the rotifer Brachionus plicatilis by a series of culture experiments. Rotifers exposed to 0.1% ethanol lived about 20% longer and the first egg-bearing individual appeared about 30 min earlier in the ethanol-exposed group than the control group. The lifetime fecundity was approximately 1.3-fold higher in the ethanol-exposed group than the control group, but the difference was not statistically significant. Ethanol exposure also increased survival time under 0.5 mM hydrogen peroxide. Furthermore, in the batch culture experiment, the maximum population density of ethanol-exposed groups was 1.7 times higher than that of control groups. Overall, these life history alterations were similar to those in glycerol-treated rotifers previously reported, suggesting a common mechanistic basis. Identification of the mechanism of ethanol action will enable future evaluation of effects of increased ethanol release on the organisms in the aquatic environment.

ACS Style

Takuma Udo; Patrick S. Guissou; Hideki Ushio; Gen Kaneko. Ethanol extends lifespan of the rotifer Brachionus plicatilis. Hydrobiologia 2018, 844, 183 -190.

AMA Style

Takuma Udo, Patrick S. Guissou, Hideki Ushio, Gen Kaneko. Ethanol extends lifespan of the rotifer Brachionus plicatilis. Hydrobiologia. 2018; 844 (1):183-190.

Chicago/Turabian Style

Takuma Udo; Patrick S. Guissou; Hideki Ushio; Gen Kaneko. 2018. "Ethanol extends lifespan of the rotifer Brachionus plicatilis." Hydrobiologia 844, no. 1: 183-190.

Journal article
Published: 28 September 2017 in British Journal of Nutrition
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Four isonitrogenous and isoenergetic purified diets containing free arachidonic acid (ARA) or EPA (control group), 0·30 % ARA, 0·30 % EPA and 0·30 % ARA+EPA (equivalent) were designed to feed juvenile grass carp (10·21 (sd 0·10) g) for 10 weeks. Only the EPA group presented better growth performance compared with the control group (PPPPFAS), diacylglycerol O-acyltransferase and apoE gene expression in the hepatopancreas decreased in fish fed ARA and EPA, but only the ARA group exhibited increased mRNA level of adipose TAG lipase (ATGL) (PPα, LPL and FAS, and increased levels of the lipid catabolic genes PPARα, ATGL, hormone-sensitive lipase and carnitine palmitoyltransferase 1 (CPT-1) in IPF, whereas the EPA group only increased PPARα and CPT-1 mRNA expression and showed less levels than the ARA group. Overall, dietary EPA is beneficial to the growth performance, whereas ARA is more potent in inducing lipolysis and inhibiting adipogenesis, especially in IPF. Meanwhile, dietary ARA and EPA showed the similar preference in esterification and the improvement in antioxidant response.

ACS Style

Jing-Jing Tian; Cai-Xia Lei; Hong Ji; Gen Kaneko; Ji-Shu Zhou; Hai-Bo Yu; Yang Li; Er-Meng Yu; Jun Xie. Comparative analysis of effects of dietary arachidonic acid and EPA on growth, tissue fatty acid composition, antioxidant response and lipid metabolism in juvenile grass carp, Ctenopharyngodon idellus. British Journal of Nutrition 2017, 118, 411 -422.

AMA Style

Jing-Jing Tian, Cai-Xia Lei, Hong Ji, Gen Kaneko, Ji-Shu Zhou, Hai-Bo Yu, Yang Li, Er-Meng Yu, Jun Xie. Comparative analysis of effects of dietary arachidonic acid and EPA on growth, tissue fatty acid composition, antioxidant response and lipid metabolism in juvenile grass carp, Ctenopharyngodon idellus. British Journal of Nutrition. 2017; 118 (6):411-422.

Chicago/Turabian Style

Jing-Jing Tian; Cai-Xia Lei; Hong Ji; Gen Kaneko; Ji-Shu Zhou; Hai-Bo Yu; Yang Li; Er-Meng Yu; Jun Xie. 2017. "Comparative analysis of effects of dietary arachidonic acid and EPA on growth, tissue fatty acid composition, antioxidant response and lipid metabolism in juvenile grass carp, Ctenopharyngodon idellus." British Journal of Nutrition 118, no. 6: 411-422.

Book chapter
Published: 23 September 2017 in Fish Population Dynamics, Monitoring, and Management
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Gen Kaneko; Tatsuki Yoshinaga; Atsushi Hagiwara. Aging and Lifespan in the Rotifer. Fish Population Dynamics, Monitoring, and Management 2017, 111 -128.

AMA Style

Gen Kaneko, Tatsuki Yoshinaga, Atsushi Hagiwara. Aging and Lifespan in the Rotifer. Fish Population Dynamics, Monitoring, and Management. 2017; ():111-128.

Chicago/Turabian Style

Gen Kaneko; Tatsuki Yoshinaga; Atsushi Hagiwara. 2017. "Aging and Lifespan in the Rotifer." Fish Population Dynamics, Monitoring, and Management , no. : 111-128.