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Dr. Lassaad Belbahri
University of neuchatel

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0 Microbiology
0 metabarcoding
0 Plant -Microbe Interaction
0 transcriptomic analysis

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Journal article
Published: 13 July 2021 in Forests
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In natural environments, plants develop adaptive mechanisms at the cellular and molecular levels to cope with many external factors, e.g., insects and soil pathogens. We studied physiological stress induced by different levels of foliage removal (defoliation 30% and 60%) and by infection of root rot pathogen Phytophthora plurivora on the common Polish tree species, Betula pendula, grown in an open greenhouse. This study showed that P. plurivora damaged the root system which negatively impacted all morphological parameters. However the loss of 30% of the leaves had a positive effect on defense responses. Chlorophyll-a fluorescence parameters indicated a decrease in photosynthetic efficiency in defoliated plants, but plants inoculated with the pathogen had a higher performance index showing increased vigor of the infected plant than birches. The study demonstrated the intense immunity response of birch to P. plurivora through the expression of Hsp90 and Hsp83 genes. The trees weakened by P. plurivora became more susceptible to infection by Erysiphe ornata var. ornata.

ACS Style

Daria Berezovska; Tomasz Oszako; Tadeusz Malewski; Marcin Stocki; Aleh Marozau; Natalia Stocka; Warren Moser; Larry Baggett; Lassaad Belbahri; Justyna Nowakowska. Effect of Defoliation on the Defense Reactions of Silver Birch (Betula pendula) Infected with Phytophthora plurivora. Forests 2021, 12, 910 .

AMA Style

Daria Berezovska, Tomasz Oszako, Tadeusz Malewski, Marcin Stocki, Aleh Marozau, Natalia Stocka, Warren Moser, Larry Baggett, Lassaad Belbahri, Justyna Nowakowska. Effect of Defoliation on the Defense Reactions of Silver Birch (Betula pendula) Infected with Phytophthora plurivora. Forests. 2021; 12 (7):910.

Chicago/Turabian Style

Daria Berezovska; Tomasz Oszako; Tadeusz Malewski; Marcin Stocki; Aleh Marozau; Natalia Stocka; Warren Moser; Larry Baggett; Lassaad Belbahri; Justyna Nowakowska. 2021. "Effect of Defoliation on the Defense Reactions of Silver Birch (Betula pendula) Infected with Phytophthora plurivora." Forests 12, no. 7: 910.

Review
Published: 06 July 2021 in Applied Sciences
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Natural dyes have been used from ancient times for multiple purposes, most importantly in the field of textile dying. The increasing demand and excessive costs of natural dye extraction engendered the discovery of synthetic dyes from petrochemical compounds. Nowadays, they are dominating the textile market, with nearly 8 × 105 tons produced per year due to their wide range of color pigments and consistent coloration. Textile industries consume huge amounts of water in the dyeing processes, making it hard to treat the enormous quantities of this hazardous wastewater. Thus, they have harmful impacts when discharged in non-treated or partially treated forms in the environment (air, soil, plants and water), causing several human diseases. In the present work we focused on synthetic dyes. We started by studying their classification which depended on the nature of the manufactured fiber (cellulose, protein and synthetic fiber dyes). Then, we mentioned the characteristics of synthetic dyes, however, we focused more on their negative impacts on the ecosystem (soil, plants, water and air) and on humans. Lastly, we discussed the applied physical, chemical and biological strategies solely or in combination for textile dye wastewater treatments. Additionally, we described the newly established nanotechnology which achieves complete discharge decontamination.

ACS Style

Houda Ben Slama; Ali Chenari Bouket; Zeinab Pourhassan; Faizah N. Alenezi; Allaoua Silini; Hafsa Cherif-Silini; Tomasz Oszako; Lenka Luptakova; Patrycja Golińska; Lassaad Belbahri. Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences 2021, 11, 6255 .

AMA Style

Houda Ben Slama, Ali Chenari Bouket, Zeinab Pourhassan, Faizah N. Alenezi, Allaoua Silini, Hafsa Cherif-Silini, Tomasz Oszako, Lenka Luptakova, Patrycja Golińska, Lassaad Belbahri. Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences. 2021; 11 (14):6255.

Chicago/Turabian Style

Houda Ben Slama; Ali Chenari Bouket; Zeinab Pourhassan; Faizah N. Alenezi; Allaoua Silini; Hafsa Cherif-Silini; Tomasz Oszako; Lenka Luptakova; Patrycja Golińska; Lassaad Belbahri. 2021. "Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods." Applied Sciences 11, no. 14: 6255.

Journal article
Published: 31 May 2021 in Applied Sciences
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Diverse vegetable oils are extracted from oleagenic trees and plants all over the world. In particular, olive oil represents a strategic socio-economic branch in the Mediterranean countries. These countries use either two or three-phase olive oil extraction systems. In this work, we focus on the by-products from three-phase olive oil extraction, which are the liquid olive mill wastewater (OMW) and the solid olive mill pomace (OMP) rejected in evaporative ponds. The disposal of this recalcitrant waste poses environmental problems such as the death of different species of insects and animals. In-depth ICP-OES analysis of the heavy metal composition of OMW and OMP revealed the presence of many metals ranging from non-toxic to highly toxic. The LC-HRMS characterization of these by-products indicated the presence of several secondary metabolites harmful to humans or to the environment. Thus, we aimed to identify OMW and OMP indigenous microbiota through metagenomics. The bacterial population was dominated by the Acetobacter (49.7%), Gluconobacter (17.3%), Gortzia (13.7%) and Nardonalla (5.3%) genera. The most abundant fungal genera were Nakazawaea, Saccharomyces, Lachancea and Candida. These microbial genera are responsible for OMW, OMP and soil toxicity alleviation.

ACS Style

Houda Ben Slama; Ali Chenari Bouket; Faizah N. Alenezi; Ameur Khardani; Lenka Luptakova; Armelle Vallat; Tomasz Oszako; Mostafa E. Rateb; Lassaad Belbahri. Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation. Applied Sciences 2021, 11, 5131 .

AMA Style

Houda Ben Slama, Ali Chenari Bouket, Faizah N. Alenezi, Ameur Khardani, Lenka Luptakova, Armelle Vallat, Tomasz Oszako, Mostafa E. Rateb, Lassaad Belbahri. Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation. Applied Sciences. 2021; 11 (11):5131.

Chicago/Turabian Style

Houda Ben Slama; Ali Chenari Bouket; Faizah N. Alenezi; Ameur Khardani; Lenka Luptakova; Armelle Vallat; Tomasz Oszako; Mostafa E. Rateb; Lassaad Belbahri. 2021. "Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation." Applied Sciences 11, no. 11: 5131.

Journal article
Published: 20 April 2021 in Marine Drugs
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Epicotripeptin (1), a new cyclic tripeptide along with four known cyclic dipeptides (2–5) and one acetamide derivative (6) were isolated from seagrass-associated endophytic fungus Epicoccum nigrum M13 recovered from the Red Sea. Additionally, two new compounds, cyclodidepsipeptide phragamide A (7) and trioxobutanamide derivative phragamide B (8), together with eight known compounds (9–16), were isolated from plant-derived endophyte Alternaria alternata 13A collected from a saline lake of Wadi El Natrun depression in the Sahara Desert. The structures of the isolated compounds were determined based on the 1D and 2D NMR spectroscopic data, HRESIMS data, and a comparison with the reported literature. The absolute configurations of 1 and 7 were established by advanced Marfey’s and Mosher’s ester analyses. The antimicrobial screening indicated that seven of the tested compounds exhibited considerable (MIC range of 2.5–5 µg/mL) to moderate (10–20 µg/mL) antibacterial effect against the tested Gram-positive strains and moderate to weak (10–30 µg/mL) antibacterial effect against Gram-negative strains. Most of the compounds exhibited weak or no activity against the tested Gram-negative strains. On the other hand, four of the tested compounds showed considerable antibiofilm effects against biofilm forming Gram-positive and Gram-negative strains.

ACS Style

M. Qader; Ahmed Hamed; Sylvia Soldatou; Mohamed Abdelraof; Mohamed Elawady; Ahmed Hassane; Lassaad Belbahri; Rainer Ebel; Mostafa Rateb. Antimicrobial and Antibiofilm Activities of the Fungal Metabolites Isolated from the Marine Endophytes Epicoccum nigrum M13 and Alternaria alternata 13A. Marine Drugs 2021, 19, 232 .

AMA Style

M. Qader, Ahmed Hamed, Sylvia Soldatou, Mohamed Abdelraof, Mohamed Elawady, Ahmed Hassane, Lassaad Belbahri, Rainer Ebel, Mostafa Rateb. Antimicrobial and Antibiofilm Activities of the Fungal Metabolites Isolated from the Marine Endophytes Epicoccum nigrum M13 and Alternaria alternata 13A. Marine Drugs. 2021; 19 (4):232.

Chicago/Turabian Style

M. Qader; Ahmed Hamed; Sylvia Soldatou; Mohamed Abdelraof; Mohamed Elawady; Ahmed Hassane; Lassaad Belbahri; Rainer Ebel; Mostafa Rateb. 2021. "Antimicrobial and Antibiofilm Activities of the Fungal Metabolites Isolated from the Marine Endophytes Epicoccum nigrum M13 and Alternaria alternata 13A." Marine Drugs 19, no. 4: 232.

Review
Published: 15 April 2021 in Sustainability
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Plant growth promoting bacteria (PGPB) have been the target of intensive research studies toward their efficient use in the field as biofertilizers, biocontrol, and bioremediation agents among numerous other applications. Recent trends in the field of PGPB research led to the development of versatile multifaceted PGPB that can be used in different field conditions such as biocontrol of plant pathogens in metal contaminated soils. Unfortunately, all these research efforts lead to the development of PGPB that failed to perform in salty environments. Therefore, it is urgently needed to address this drawback of these PGPB toward their efficient performance in salinity context. In this paper we provide a review of state-of-the-art research in the field of PGPB and propose a road map for the development of next generation versatile and multifaceted PGPB that can perform in salinity. Beyond soil desalinization, our study paves the way towards the development of PGPB able to provide services in diverse salty environments such as heavy metal contaminated, or pathogen threatened. Smart development of salinity adapted next generation biofertilizers will inevitably allow for mitigation and alleviation of biotic and abiotic threats to plant productivity in salty environments.

ACS Style

Hafsa Cherif-Silini; Allaoua Silini; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Nawel Bouremani; Justyna Anna Nowakowska; Tomasz Oszako; Lassaad Belbahri. Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application. Sustainability 2021, 13, 4422 .

AMA Style

Hafsa Cherif-Silini, Allaoua Silini, Ali Chenari Bouket, Faizah N. Alenezi, Lenka Luptakova, Nawel Bouremani, Justyna Anna Nowakowska, Tomasz Oszako, Lassaad Belbahri. Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application. Sustainability. 2021; 13 (8):4422.

Chicago/Turabian Style

Hafsa Cherif-Silini; Allaoua Silini; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Nawel Bouremani; Justyna Anna Nowakowska; Tomasz Oszako; Lassaad Belbahri. 2021. "Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application." Sustainability 13, no. 8: 4422.

Journal article
Published: 01 April 2021 in Materials Today Communications
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Nanocomposite films of polysulfone (PSU) with different amounts (0.00–1.00 wt%) of a nanohybrid blend of graphene oxide (GO) and silver nanoparticles (AgNPs) obtained by a solution-blending method showed good properties. Tensile tests revealed that the PSU/GO-Ag nanocomposite films prepared have higher Young’s modulus and tensile strength compared to the neat PSU films. The optimistic effects on the thermal stability, morphological structure as well as the hydrophilicity character of all the nanocomposite films was very clear furthermore, antibacterial testing results showed that the nanocomposite films prepared have a significant bactericidal capability, at very low GO-Ag loading (0.2 wt%), against both Gram negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) strains. The characteristics of these nanocomposites based on the various results obtained in this study would give them a strong impetus for their use in many areas, particularly in the medical field.

ACS Style

Samir Bouchareb; Rachida Doufnoune; Farid Riahi; Hafsa Cherif-Silini; Lassaad Belbahri. High performance of polysulfone/graphene oxide-silver nanocomposites with excellent antibacterial capability for medical applications. Materials Today Communications 2021, 27, 102297 .

AMA Style

Samir Bouchareb, Rachida Doufnoune, Farid Riahi, Hafsa Cherif-Silini, Lassaad Belbahri. High performance of polysulfone/graphene oxide-silver nanocomposites with excellent antibacterial capability for medical applications. Materials Today Communications. 2021; 27 ():102297.

Chicago/Turabian Style

Samir Bouchareb; Rachida Doufnoune; Farid Riahi; Hafsa Cherif-Silini; Lassaad Belbahri. 2021. "High performance of polysulfone/graphene oxide-silver nanocomposites with excellent antibacterial capability for medical applications." Materials Today Communications 27, no. : 102297.

Journal article
Published: 21 March 2021 in Molecules
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Severe acute respiratory syndrome coronavirus (SARS-CoV-2) disease is a global rapidly spreading virus showing very high rates of complications and mortality. Till now, there is no effective specific treatment for the disease. Aloe is a rich source of isolated phytoconstituents that have an enormous range of biological activities. Since there are no available experimental techniques to examine these compounds for antiviral activity against SARS-CoV-2, we employed an in silico approach involving molecular docking, dynamics simulation, and binding free energy calculation using SARS-CoV-2 essential proteins as main protease and spike protein to identify lead compounds from Aloe that may help in novel drug discovery. Results retrieved from docking and molecular dynamics simulation suggested a number of promising inhibitors from Aloe. Root mean square deviation (RMSD) and root mean square fluctuation (RMSF) calculations indicated that compounds 132, 134, and 159 were the best scoring compounds against main protease, while compounds 115, 120, and 131 were the best scoring ones against spike glycoprotein. Compounds 120 and 131 were able to achieve significant stability and binding free energies during molecular dynamics simulation. In addition, the highest scoring compounds were investigated for their pharmacokinetic properties and drug-likeness. The Aloe compounds are promising active phytoconstituents for drug development for SARS-CoV-2.

ACS Style

Mohamed Abouelela; Hamdy Assaf; Reda Abdelhamid; Ehab Elkhyat; Ahmed Sayed; Tomasz Oszako; Lassaad Belbahri; Ahmed El Zowalaty; Mohamed Abdelkader. Identification of Potential SARS-CoV-2 Main Protease and Spike Protein Inhibitors from the Genus Aloe: An In Silico Study for Drug Development. Molecules 2021, 26, 1767 .

AMA Style

Mohamed Abouelela, Hamdy Assaf, Reda Abdelhamid, Ehab Elkhyat, Ahmed Sayed, Tomasz Oszako, Lassaad Belbahri, Ahmed El Zowalaty, Mohamed Abdelkader. Identification of Potential SARS-CoV-2 Main Protease and Spike Protein Inhibitors from the Genus Aloe: An In Silico Study for Drug Development. Molecules. 2021; 26 (6):1767.

Chicago/Turabian Style

Mohamed Abouelela; Hamdy Assaf; Reda Abdelhamid; Ehab Elkhyat; Ahmed Sayed; Tomasz Oszako; Lassaad Belbahri; Ahmed El Zowalaty; Mohamed Abdelkader. 2021. "Identification of Potential SARS-CoV-2 Main Protease and Spike Protein Inhibitors from the Genus Aloe: An In Silico Study for Drug Development." Molecules 26, no. 6: 1767.

Journal article
Published: 12 March 2021 in Toxics
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Dimethyl sulfoxide (DMSO) is widely used as a solvent for small hydrophobic drug molecules. However, the safe volume allowing to avoid its embryotoxic effect has been poorly studied. In this study, we documented the effects of dimethyl sulfoxide (DMSO) in the developing chicken embryo at morphological, histological, and molecular levels. We focused on the developing chicken liver as the main organ involved in the process of detoxification. In our study, 100% DMSO was administered subgerminally onto the eggshell membrane (membrana papyracea) at various volumes (5, 10, 15, 20, 25, 30, 35, and 50 µL) on 4th embryonic day (ED). We focused on histopathological alterations of the liver structure, and noticed the overall impact of DMSO on developing chicken embryos (embryotoxicity, malformation). At the molecular level, we studied cytochrome P450 complex (CYP) isoform’s activities in relation to changes of CYP1A5, CYP3A37, and CYP3A80 gene expression. Total embryotoxicity after application of different doses of DMSO on ED 4, and the embryo lethality increased with increasing DMSO amounts. Overall mortality after DMSO administration ranged below 33%. Mortality was increased with higher amounts of DMSO, mainly from 20 µL. The highest mortality was observed for the highest dose of DMSO over 35 µL. The results also showed a decrease in body weight with increased application volumes of DMSO. At the histological level, we observed mainly the presence of lipid droplets and dilated bile canaliculi and sinusoids in samples over the administration of 25 µL of DMSO. While these findings were not statistically significant, DMSO treatment caused a significant different up-regulation of mRNA expression in all studied genes. For CYP1A5, CYP3A37, and CYP3A80 DMSO volumes needed were 15 µL, 10 µL, and 20 µL, respectively. A significant down-regulation of all studied CYP isoform was detected after application of a DMSO dose of 5 µL. Regarding the morphological results, we can assume that the highest safe dose of DMSO without affecting chicken embryo development and its liver is up to 10 µL. This conclusion is corroborated with the presence of number of malformations and body weight reduction, which correlates with histological findings. Moreover, the gene expression results showed that even the lowest administered DMSO volume could affect hepatocytes at the molecular level causing down-regulation of cytochrome P450 complex (CYP1A5, CYP3A37, CYP3A80).

ACS Style

Lenka Luptakova; Simona Dvorcakova; Zuzana Demcisakova; Lassaad Belbahri; Katarina Holovska; Eva Petrovova. Dimethyl Sulfoxide: Morphological, Histological, and Molecular View on Developing Chicken Liver. Toxics 2021, 9, 55 .

AMA Style

Lenka Luptakova, Simona Dvorcakova, Zuzana Demcisakova, Lassaad Belbahri, Katarina Holovska, Eva Petrovova. Dimethyl Sulfoxide: Morphological, Histological, and Molecular View on Developing Chicken Liver. Toxics. 2021; 9 (3):55.

Chicago/Turabian Style

Lenka Luptakova; Simona Dvorcakova; Zuzana Demcisakova; Lassaad Belbahri; Katarina Holovska; Eva Petrovova. 2021. "Dimethyl Sulfoxide: Morphological, Histological, and Molecular View on Developing Chicken Liver." Toxics 9, no. 3: 55.

Chapter
Published: 05 March 2021 in Sustainable Development and Biodiversity
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Endophytes have been known for more than a century and recent studies highlighted their endless potentialities in plant growth promotion through several direct and indirect mechanisms. Competent microbial endophytes have been acknowledged in several fields including medicine, industry, pharmacology, bioremediation, and phytoremediation of pollutants due to their safe handling and environment-friendly effects. Modern genomic approaches are considered an effective tool to get a better knowledge of the microbial modes of action. This chapter focuses on endophytes taxonomic affinities and lifestyle, their plant growth-promoting mechanisms, their applications as well as the potential of emerging “omic” tools including genomic, transcriptomic, metabolomics, and proteomics to shed the light on the wealth of their genomic and metabolic potentialities.

ACS Style

Houda Ben Slama; Hafsa Cherif-Silini; Ali Chenari Bouket; Allaoua Silini; Faizah N. Alenezi; Lenka Luptakova; Armelle Vallat; Lassaad Belbahri. Biotechnology and Bioinformatics of Endophytes in Biocontrol, Bioremediation, and Plant Growth Promotion. Sustainable Development and Biodiversity 2021, 181 -205.

AMA Style

Houda Ben Slama, Hafsa Cherif-Silini, Ali Chenari Bouket, Allaoua Silini, Faizah N. Alenezi, Lenka Luptakova, Armelle Vallat, Lassaad Belbahri. Biotechnology and Bioinformatics of Endophytes in Biocontrol, Bioremediation, and Plant Growth Promotion. Sustainable Development and Biodiversity. 2021; ():181-205.

Chicago/Turabian Style

Houda Ben Slama; Hafsa Cherif-Silini; Ali Chenari Bouket; Allaoua Silini; Faizah N. Alenezi; Lenka Luptakova; Armelle Vallat; Lassaad Belbahri. 2021. "Biotechnology and Bioinformatics of Endophytes in Biocontrol, Bioremediation, and Plant Growth Promotion." Sustainable Development and Biodiversity , no. : 181-205.

Article
Published: 01 March 2021 in Current Microbiology
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Biotic and abiotic stresses are severely limiting plant production and productivity. Of notable importance is salt stress that not only limits plant growth and survival, but affects the soil fertility and threatens agricultural ecosystems sustainability. The problem is exacerbated in fragile arid and semi-arid areas where high evaporation, low precipitation and the use of salty water for irrigation is accelerating soil salinization. Legumes, considered very nutritious foods for people and providing essential nutrients for ecosystems are a fundamental element of sustainable agriculture. They can restore soil health by their ability to fix nitrogen in a symbiotic interaction with the rhizobia of the soil. However, salt stress is severely limiting productivity and nitrogen fixation ability in legumes. Plant growth-promoting rhizobacteria (PGPR) and mainly actinobacteria promote plant growth by producing phytohormones, siderophores, antibiotics and antifungal compounds, solubilizing phosphate and providing antagonism to phytopathogenic microorganisms. In addition, actinobacteria have beneficial effects on nodulation and growth of legumes. In this study, actinobacteria isolated from different niches and having PGP activities were used in co-inoculation experiments with rhizobia in Medicago sativa plants rhizosphere submitted to salt stress. The results indicate that drought- and salinity-tolerant Actinobacteria with multiple PGP traits can potentially increase alfalfa growth under saline conditions, in the presence or absence of symbiotic rhizobial bacteria. Actinobacteria discovered in this study can, therefore, be suitable biofertilizers in the formulation of agricultural products improving plant development, health and productivity in saline soils, a necessary alternative for modern agriculture and sustainable development.

ACS Style

Samira Saidi; Hafsa Cherif-Silini; Ali Chenari Bouket; Allaoua Silini; Manal Eshelli; Lenka Luptakova; Faizah N. Alenezi; Lassaad Belbahri. Improvement of Medicago sativa Crops Productivity by the Co-inoculation of Sinorhizobium meliloti–Actinobacteria Under Salt Stress. Current Microbiology 2021, 78, 1344 -1357.

AMA Style

Samira Saidi, Hafsa Cherif-Silini, Ali Chenari Bouket, Allaoua Silini, Manal Eshelli, Lenka Luptakova, Faizah N. Alenezi, Lassaad Belbahri. Improvement of Medicago sativa Crops Productivity by the Co-inoculation of Sinorhizobium meliloti–Actinobacteria Under Salt Stress. Current Microbiology. 2021; 78 (4):1344-1357.

Chicago/Turabian Style

Samira Saidi; Hafsa Cherif-Silini; Ali Chenari Bouket; Allaoua Silini; Manal Eshelli; Lenka Luptakova; Faizah N. Alenezi; Lassaad Belbahri. 2021. "Improvement of Medicago sativa Crops Productivity by the Co-inoculation of Sinorhizobium meliloti–Actinobacteria Under Salt Stress." Current Microbiology 78, no. 4: 1344-1357.

Journal article
Published: 24 January 2021 in Applied Sciences
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There is a great interest in mitigating soil salinity that limits plant growth and productivity. In this study, eighty-nine strains were isolated from the rhizosphere and endosphere of two halophyte species (Suaeda mollis and Salsola tetrandra) collected from three chotts in Algeria. They were screened for diverse plant growth-promoting traits, antifungal activity and tolerance to different physico-chemical conditions (pH, PEG, and NaCl) to evaluate their efficiency in mitigating salt stress and enhancing the growth of Arabidopsis thaliana and durum wheat under NaCl–stress conditions. Three bacterial strains BR5, OR15, and RB13 were finally selected and identified as Bacillus atropheus. The Bacterial strains (separately and combined) were then used for inoculating Arabidopsis thaliana and durum wheat during the seed germination stage under NaCl stress conditions. Results indicated that inoculation of both plant spp. with the bacterial strains separately or combined considerably improved the growth parameters. Three soils with different salinity levels (S1 = 0.48, S2 = 3.81, and S3 = 2.80 mS/cm) were used to investigate the effects of selected strains (BR5, OR15, and RB13; separately and combined) on several growth parameters of wheat plants. The inoculation (notably the multi-strain consortium) proved a better approach to increase the chlorophyll and carotenoid contents as compared to control plants. However, proline content, lipid peroxidation, and activities of antioxidant enzymes decreased after inoculation with the plant growth-promoting rhizobacteria (PGPR) that can attenuate the adverse effects of salt stress by reducing the reactive oxygen species (ROS) production. These results indicated that under saline soil conditions, halotolerant PGPR strains are promising candidates as biofertilizers under salt stress conditions.

ACS Style

Souhila Kerbab; Allaoua Silini; Ali Chenari Bouket; Hafsa Cherif-Silini; Manal Eshelli; Nour El Houda Rabhi; Lassaad Belbahri. Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria. Applied Sciences 2021, 11, 1034 .

AMA Style

Souhila Kerbab, Allaoua Silini, Ali Chenari Bouket, Hafsa Cherif-Silini, Manal Eshelli, Nour El Houda Rabhi, Lassaad Belbahri. Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria. Applied Sciences. 2021; 11 (3):1034.

Chicago/Turabian Style

Souhila Kerbab; Allaoua Silini; Ali Chenari Bouket; Hafsa Cherif-Silini; Manal Eshelli; Nour El Houda Rabhi; Lassaad Belbahri. 2021. "Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria." Applied Sciences 11, no. 3: 1034.

Review
Published: 26 December 2020 in Plants
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The acute respiratory syndrome caused by the novel coronavirus (SARS-CoV-2) caused severe panic all over the world. The coronavirus (COVID-19) outbreak has already brought massive human suffering and major economic disruption and unfortunately, there is no specific treatment for COVID-19 so far. Herbal medicines and purified natural products can provide a rich resource for novel antiviral drugs. Therefore, in this review, we focused on the sterols and triterpenes as potential candidates derived from natural sources with well-reported in vitro efficacy against numerous types of viruses. Moreover, we compiled from these reviewed compounds a library of 162 sterols and triterpenes that was subjected to a computer-aided virtual screening against the active sites of the recently reported SARS-CoV-2 protein targets. Interestingly, the results suggested some compounds as potential drug candidates for the development of anti-SARS-CoV-2 therapeutics.

ACS Style

Nourhan Hisham Shady; Khayrya A. Youssif; Ahmed M. Sayed; Lassaad Belbahri; Tomasz Oszako; Hossam M. Hassan; Usama Ramadan Abdelmohsen. Sterols and Triterpenes: Antiviral     Potential Supported by In-Silico Analysis. Plants 2020, 10, 41 .

AMA Style

Nourhan Hisham Shady, Khayrya A. Youssif, Ahmed M. Sayed, Lassaad Belbahri, Tomasz Oszako, Hossam M. Hassan, Usama Ramadan Abdelmohsen. Sterols and Triterpenes: Antiviral     Potential Supported by In-Silico Analysis. Plants. 2020; 10 (1):41.

Chicago/Turabian Style

Nourhan Hisham Shady; Khayrya A. Youssif; Ahmed M. Sayed; Lassaad Belbahri; Tomasz Oszako; Hossam M. Hassan; Usama Ramadan Abdelmohsen. 2020. "Sterols and Triterpenes: Antiviral     Potential Supported by In-Silico Analysis." Plants 10, no. 1: 41.

Article
Published: 21 November 2020 in European Journal of Plant Pathology
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The protective effects of Trichoderma asperellum IZR D-11 as a biocontrol agent against the powdery mildew Erysiphe alphitoides infecting leaves of Quercus robur were evaluated for the first time. A strain of Trichoderma had earlier been isolated in Belarus, and was identified in this study as T. asperellum by sequencing of three genomic markers: internal transcribed spacer, translation elongation factor 1 alpha and RNA polymerase II subunit 2, with over 99.2% identity to corresponding genomic sequences in GenBank. When applied once in the first year just after onset of powdery mildew disease, T asperellum IZR D-11 reduced disease progression and continued to reduce powdery mildew levels during the following three years. Photosynthetic activity as represented by chlorophyll fluorescence measured in oak seedlings was increased in treated plants, and greater assimilate production was also found. The use of this antagonistic fungus increased the total water content in oak leaves suggesting that T. asperellum IZR D-11 can serve as a preventive measure to reduce energy losses in the process of water transpiration. GC-MS analysis detected 49 volatile compounds in the headspace of pure cultures of T. asperellum. Sesquiterpenes represented mainly by daucene, dauca-4(11),8-diene and isodaucene were the largest group of compounds emitted. We speculate that these volatiles from T. asperellum IZR D-11 may be involved in induced resistance in the plant, but further research is needed. The above results suggest that T. asperellum strain IZR D-11 has potential as a biocontrol agent of oak powdery mildew in forest nurseries.

ACS Style

Tomasz Oszako; Dmitry Voitka; Marcin Stocki; Natalia Stocka; Justyna Anna Nowakowska; Anna Linkiewicz; Tom Hsiang; Lassaâd Belbahri; Daria Berezovska; Tadeusz Malewski. Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides. European Journal of Plant Pathology 2020, 159, 295 -308.

AMA Style

Tomasz Oszako, Dmitry Voitka, Marcin Stocki, Natalia Stocka, Justyna Anna Nowakowska, Anna Linkiewicz, Tom Hsiang, Lassaâd Belbahri, Daria Berezovska, Tadeusz Malewski. Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides. European Journal of Plant Pathology. 2020; 159 (2):295-308.

Chicago/Turabian Style

Tomasz Oszako; Dmitry Voitka; Marcin Stocki; Natalia Stocka; Justyna Anna Nowakowska; Anna Linkiewicz; Tom Hsiang; Lassaâd Belbahri; Daria Berezovska; Tadeusz Malewski. 2020. "Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides." European Journal of Plant Pathology 159, no. 2: 295-308.

Journal article
Published: 19 November 2020 in Environment International
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Protists dominate eukaryotic diversity and play key functional roles in all ecosystems, particularly by catalyzing carbon and nutrient cycling. To date, however, a comparative analysis of their taxonomic and functional diversity that compares the major ecosystems on Earth (soil, freshwater and marine systems) is missing. Here, we present a comparison of protist diversity based on standardized high throughput 18S rRNA gene sequencing of soil, freshwater and marine environmental DNA. Soil and freshwater protist communities were more similar to each other than to marine protist communities, with virtually no overlap of Operational Taxonomic Units (OTUs) between terrestrial and marine habitats. Soil protists showed higher γ diversity than aquatic samples. Differences in taxonomic composition of the communities led to changes in a functional diversity among ecosystems, as expressed in relative abundance of consumers, phototrophs and parasites. Phototrophs (eukaryotic algae) dominated freshwater systems (49% of the sequences) and consumers soil and marine ecosystems (59% and 48%, respectively). The individual functional groups were composed of ecosystem- specific taxonomic groups. Parasites were equally common in all ecosystems, yet, terrestrial systems hosted more OTUs assigned to parasites of macro-organisms while aquatic systems contained mostly microbial parasitoids. Together, we show biogeographic patterns of protist diversity across major ecosystems on Earth, preparing the way for more focused studies that will help understanding the multiple roles of protists in the biosphere.

ACS Style

David Singer; Christophe V.W. Seppey; Guillaume Lentendu; Micah Dunthorn; David Bass; Lassâad Belbahri; Quentin Blandenier; Didier Debroas; G. Arjen de Groot; Colomban de Vargas; Isabelle Domaizon; Clément Duckert; Irina Izaguirre; Isabelle Koenig; Gabriela Mataloni; M. Romina Schiaffino; Edward A.D. Mitchell; Stefan Geisen; Enrique Lara. Protist taxonomic and functional diversity in soil, freshwater and marine ecosystems. Environment International 2020, 146, 106262 .

AMA Style

David Singer, Christophe V.W. Seppey, Guillaume Lentendu, Micah Dunthorn, David Bass, Lassâad Belbahri, Quentin Blandenier, Didier Debroas, G. Arjen de Groot, Colomban de Vargas, Isabelle Domaizon, Clément Duckert, Irina Izaguirre, Isabelle Koenig, Gabriela Mataloni, M. Romina Schiaffino, Edward A.D. Mitchell, Stefan Geisen, Enrique Lara. Protist taxonomic and functional diversity in soil, freshwater and marine ecosystems. Environment International. 2020; 146 ():106262.

Chicago/Turabian Style

David Singer; Christophe V.W. Seppey; Guillaume Lentendu; Micah Dunthorn; David Bass; Lassâad Belbahri; Quentin Blandenier; Didier Debroas; G. Arjen de Groot; Colomban de Vargas; Isabelle Domaizon; Clément Duckert; Irina Izaguirre; Isabelle Koenig; Gabriela Mataloni; M. Romina Schiaffino; Edward A.D. Mitchell; Stefan Geisen; Enrique Lara. 2020. "Protist taxonomic and functional diversity in soil, freshwater and marine ecosystems." Environment International 146, no. : 106262.

Journal article
Published: 21 October 2020 in Microorganisms
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Marine endophytic fungi from under-explored locations are a promising source for the discovery of new bioactivities. Different endophytic fungi were isolated from plants and marine organisms collected from Wadi El-Natrun saline lakes and the Red Sea near Hurghada, Egypt. The isolated strains were grown on three different media, and their ethyl acetate crude extracts were evaluated for their antimicrobial activity against a panel of pathogenic bacteria and fungi as well as their antioxidant properties. Results showed that most of the 32 fungal isolates initially obtained possessed antimicrobial and antioxidant activities. The most potent antimicrobial extracts were applied to three different cellulose containing fabrics to add new multifunctional properties such as ultraviolet protection and antimicrobial functionality. For textile safety, the toxicity profile of the selected fungal extract was evaluated on human fibroblasts. The 21 strains displaying bioactivity were identified on molecular basis and selected for chemical screening and dereplication, which was carried out by analysis of the MS/MS data using the Global Natural Products Social Molecular Networking (GNPS) platform. The obtained molecular network revealed molecular families of compounds commonly produced by fungal strains, and in combination with manual dereplication, further previously reported metabolites were identified as well as potentially new derivatives.

ACS Style

Ahmed Hamed; Sylvia Soldatou; M. Qader; Subha Arjunan; Kevin Miranda; Federica Casolari; Coralie Pavesi; Oluwatofunmilay Diyaolu; Bathini Thissera; Manal Eshelli; Lassaad Belbahri; Lenka Luptakova; Nabil Ibrahim; Mohamed Abdel-Aziz; Basma Eid; Mosad Ghareeb; Mostafa Rateb; Rainer Ebel. Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles. Microorganisms 2020, 8, 1617 .

AMA Style

Ahmed Hamed, Sylvia Soldatou, M. Qader, Subha Arjunan, Kevin Miranda, Federica Casolari, Coralie Pavesi, Oluwatofunmilay Diyaolu, Bathini Thissera, Manal Eshelli, Lassaad Belbahri, Lenka Luptakova, Nabil Ibrahim, Mohamed Abdel-Aziz, Basma Eid, Mosad Ghareeb, Mostafa Rateb, Rainer Ebel. Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles. Microorganisms. 2020; 8 (10):1617.

Chicago/Turabian Style

Ahmed Hamed; Sylvia Soldatou; M. Qader; Subha Arjunan; Kevin Miranda; Federica Casolari; Coralie Pavesi; Oluwatofunmilay Diyaolu; Bathini Thissera; Manal Eshelli; Lassaad Belbahri; Lenka Luptakova; Nabil Ibrahim; Mohamed Abdel-Aziz; Basma Eid; Mosad Ghareeb; Mostafa Rateb; Rainer Ebel. 2020. "Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles." Microorganisms 8, no. 10: 1617.

Journal article
Published: 31 August 2020 in Antibiotics
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Recently, the interest in plant-derived antimicrobial agents has increased. However, there are no sufficient studies dealing with their modes of action. Herein, we investigate an in-house library of common plant-based phenolic compounds for their potential antibacterial effects against the methicillin-resistant Staphylococcus aureus (MRSA), a widespread life-threatening superbug. Flavonoids, which are considered major constituents in the plant kingdom, were found to be a promising class of compounds against MRSA, particularly the non-glycosylated ones. On the other hand, the glycosylated derivatives, along with the flavonolignan silibinin A, were able to restore the inhibitory activity of ampicillin against MRSA. To explore the mode of action of this class, they were subjected to an extensive inverse virtual screening (IVS), which suggested penicillin-binding protein 2a (PBP2a) as a possible target that mediates both the antibacterial and the antibiotic-synergistic effects of this class of compounds. Further molecular docking and molecular dynamic simulation experiments were conducted to support the primary IVS and the in vitro results and to study their binding modes with PBP2a. Our findings shed a light on plant-derived natural products, notably flavonoids, as a promising and readily available source for future adjuvant antimicrobial therapy against resistant strains.

ACS Style

Hani A. Alhadrami; Ahmed A. Hamed; Hossam M. Hassan; Lassaad Belbahri; Mostafa E. Rateb; Ahmed M. Sayed. Flavonoids as Potential anti-MRSA Agents through Modulation of PBP2a: A Computational and Experimental Study. Antibiotics 2020, 9, 562 .

AMA Style

Hani A. Alhadrami, Ahmed A. Hamed, Hossam M. Hassan, Lassaad Belbahri, Mostafa E. Rateb, Ahmed M. Sayed. Flavonoids as Potential anti-MRSA Agents through Modulation of PBP2a: A Computational and Experimental Study. Antibiotics. 2020; 9 (9):562.

Chicago/Turabian Style

Hani A. Alhadrami; Ahmed A. Hamed; Hossam M. Hassan; Lassaad Belbahri; Mostafa E. Rateb; Ahmed M. Sayed. 2020. "Flavonoids as Potential anti-MRSA Agents through Modulation of PBP2a: A Computational and Experimental Study." Antibiotics 9, no. 9: 562.

Journal article
Published: 29 June 2020 in Microorganisms
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The main protease (Mpro) of the newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was subjected to hyphenated pharmacophoric-based and structural-based virtual screenings using a library of microbial natural products (>24,000 compounds). Subsequent filtering of the resulted hits according to the Lipinski’s rules was applied to select only the drug-like molecules. Top-scoring hits were further filtered out depending on their ability to show constant good binding affinities towards the molecular dynamic simulation (MDS)-derived enzyme’s conformers. Final MDS experiments were performed on the ligand–protein complexes (compounds 1–12, Table S1) to verify their binding modes and calculate their binding free energy. Consequently, a final selection of six compounds (1–6) was proposed to possess high potential as anti-SARS-CoV-2 drug candidates. Our study provides insight into the role of the Mpro structural flexibility during interactions with the possible inhibitors and sheds light on the structure-based design of anti-coronavirus disease 2019 (COVID-19) therapeutics targeting SARS-CoV-2.

ACS Style

Ahmed M. Sayed; Hani A. Alhadrami; Ahmed O. El-Gendy; Yara I. Shamikh; Lassaad Belbahri; Hossam M. Hassan; Usama Ramadan Abdelmohsen; Mostafa E. Rateb. Microbial Natural Products as Potential Inhibitors of SARS-CoV-2 Main Protease (Mpro). Microorganisms 2020, 8, 970 .

AMA Style

Ahmed M. Sayed, Hani A. Alhadrami, Ahmed O. El-Gendy, Yara I. Shamikh, Lassaad Belbahri, Hossam M. Hassan, Usama Ramadan Abdelmohsen, Mostafa E. Rateb. Microbial Natural Products as Potential Inhibitors of SARS-CoV-2 Main Protease (Mpro). Microorganisms. 2020; 8 (7):970.

Chicago/Turabian Style

Ahmed M. Sayed; Hani A. Alhadrami; Ahmed O. El-Gendy; Yara I. Shamikh; Lassaad Belbahri; Hossam M. Hassan; Usama Ramadan Abdelmohsen; Mostafa E. Rateb. 2020. "Microbial Natural Products as Potential Inhibitors of SARS-CoV-2 Main Protease (Mpro)." Microorganisms 8, no. 7: 970.

Journal article
Published: 10 February 2020 in Biomolecules
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Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, we report a rare example of the induction of silent/cryptic bacterial biosynthetic pathway by the co-culture of Durum wheat plant roots-associated bacterium Pantoea aggolomerans and date palm leaves-derived fungus Penicillium citrinum. The initial co-culture indicated a clear fungal growth inhibition which was confirmed by the promising antifungal activity of the co-culture total extract against Pc. LC-HRMS chemical profiling demonstrated a huge suppression in the production of secondary metabolites (SMs) of axenic cultures of both species with the emergence of new metabolites which were dereplicated as a series of siderophores. Large-scale co-culture fermentation led to the isolation of two new pulicatin derivatives together with six known metabolites which were characterised using HRESIMS and NMR analyses. During the in vitro antimicrobial evaluation of the isolated compounds, pulicatin H (2) exhibited the strongest antifungal activity against Pc, followed by aeruginaldehyde (1) and pulicatin F (4), hence explaining the initial growth suppression of Pc in the co-culture environment.

ACS Style

Bathini Thissera; Hani A. Alhadrami; Marwa H. A. Hassan; Hossam M. Hassan; Fathy A. Behery; Majed Bawazeer; Mohammed Yaseen; Lassaad Belbahri; Mostafa E. Rateb; Hassan. Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture. Biomolecules 2020, 10, 268 .

AMA Style

Bathini Thissera, Hani A. Alhadrami, Marwa H. A. Hassan, Hossam M. Hassan, Fathy A. Behery, Majed Bawazeer, Mohammed Yaseen, Lassaad Belbahri, Mostafa E. Rateb, Hassan. Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture. Biomolecules. 2020; 10 (2):268.

Chicago/Turabian Style

Bathini Thissera; Hani A. Alhadrami; Marwa H. A. Hassan; Hossam M. Hassan; Fathy A. Behery; Majed Bawazeer; Mohammed Yaseen; Lassaad Belbahri; Mostafa E. Rateb; Hassan. 2020. "Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture." Biomolecules 10, no. 2: 268.

Journal article
Published: 31 December 2019 in Materials Chemistry and Physics
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GO-Ag nanohybrids have attracted tremendous attention thanks to their several potential properties. In this research work, a new approach was adopted, where Triphenylphosphine (PPh3) was used as a linkage to decorate the surface of Graphene oxide (GO) nanosheets by Silver nanoparticles (AgNPs) via a simple method. The Fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction analysis (XRD), Raman spectroscopy, Thermo-gravimetric analysis (TGA), X-ray fluorescence (XRF), Zeta potential analysis, Scanning electron microscopy (SEM), and Atomic force microscopy (AFM) techniques were used to reveal that AgNPs have covered the surface of GO sheets through non covalent and permanent bonding, altering new structural and electronic properties, leading to the appearance of the oxidative stress phenomenon, which considered as a key step of the antibacterial mechanism of this kind of hybrids, causing death of both Gram-positive (Bacillus subtilis, Enterococcus faecalis, Methicillin-resistant Staphylococcus aureus - MRSA, and Staphylococcus aureus) and Gram-negative (Escherichia coli, Serratia marcescens, Shigella sp, Salmonella sp, Serratia microorganisms liquefaciens, Proteus sp, Enterobacter cloacae and Pseudomonas aeruginosa).

ACS Style

Samir Bouchareb; Rachida Doufnoune; Farid Riahi; Hafsa Cherif-Silini; Lassaad Belbahri. Non-covalent functionalization of graphene oxide using self-assembly of silver-triphenylphosphine for bactericidal formulations. Materials Chemistry and Physics 2019, 243, 122598 .

AMA Style

Samir Bouchareb, Rachida Doufnoune, Farid Riahi, Hafsa Cherif-Silini, Lassaad Belbahri. Non-covalent functionalization of graphene oxide using self-assembly of silver-triphenylphosphine for bactericidal formulations. Materials Chemistry and Physics. 2019; 243 ():122598.

Chicago/Turabian Style

Samir Bouchareb; Rachida Doufnoune; Farid Riahi; Hafsa Cherif-Silini; Lassaad Belbahri. 2019. "Non-covalent functionalization of graphene oxide using self-assembly of silver-triphenylphosphine for bactericidal formulations." Materials Chemistry and Physics 243, no. : 122598.

Journal article
Published: 23 September 2019 in Microorganisms
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The phosphogypsum (PG) endogenous bacterial community and endophytic bacterial communities of four plants growing in phosphogypsum-contaminated sites, Suaeda fruticosa (SF), Suaeda mollis (SM), Mesembryanthmum nodiflorum (MN) and Arthrocnemum indicum (AI) were investigated by amplicon sequencing. Results highlight a more diverse community of phosphogypsum than plants associated endophytic communities. Additionally, the bacterial culturable communities of phosphogypsum and associated plant endophytes were isolated and their plant-growth promotion capabilities, bioremediation potential and stress tolerance studied. Most of plant endophytes were endowed with plant growth-promoting (PGP) activities and phosphogypsum communities and associated plants endophytes proved highly resistant to salt, metal and antibiotic stress. They also proved very active in bioremediation of phosphogypsum and other organic and inorganic environmental pollutants. Genome sequencing of five members of the phosphogypsum endogenous community showed that they belong to the recently described species Bacillus albus (BA). Genome mining of BA allowed the description of pollutant degradation and stress tolerance mechanisms. Prevalence of this tool box in the core, accessory and unique genome allowed to conclude that accessory and unique genomes are critical for the dynamics of strain acquisition of bioremediation abilities. Additionally, secondary metabolites (SM) active in bioremediation such as petrobactin have been characterized. Taken together, our results reveal hidden untapped valuable bacterial actors for waste remediation.

ACS Style

Fedia Ben Mefteh; Ali Chenari Bouket; Amal Daoud; Lenka Luptakova; Faizah N. Alenezi; Neji Gharsallah; Lassaad Belbahri; Ben Mefteh. Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation. Microorganisms 2019, 7, 382 .

AMA Style

Fedia Ben Mefteh, Ali Chenari Bouket, Amal Daoud, Lenka Luptakova, Faizah N. Alenezi, Neji Gharsallah, Lassaad Belbahri, Ben Mefteh. Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation. Microorganisms. 2019; 7 (10):382.

Chicago/Turabian Style

Fedia Ben Mefteh; Ali Chenari Bouket; Amal Daoud; Lenka Luptakova; Faizah N. Alenezi; Neji Gharsallah; Lassaad Belbahri; Ben Mefteh. 2019. "Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation." Microorganisms 7, no. 10: 382.