This page has only limited features, please log in for full access.

Unclaimed
Tamás Kőszegi
Department of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja 13, H-7624 Pécs, Hungary

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 11 June 2021 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Mycotoxins are toxic metabolites of filamentous fungi. Previous studies demonstrated the co-occurrence of Fusarium and Alternaria toxins, including zearalenone (ZEN), ZEN metabolites, and alternariol (AOH). These xenoestrogenic mycotoxins appear in soy-based meals and dietary supplements, resulting in the co-exposure to ZEN and AOH with the phytoestrogen genistein (GEN). In this study, the cytotoxic and estrogenic effects of ZEN, reduced ZEN metabolites, AOH, and GEN are examined to evaluate their individual and combined impacts. Our results demonstrate that reduced ZEN metabolites, AOH, and GEN can aggravate ZEN-induced toxicity; in addition, the compounds tested exerted mostly synergism or additive combined effects regarding cytotoxicity and/or estrogenicity. Therefore, these observations underline the importance and the considerable risk of mycotoxin co-exposure and the combined effects of mycoestrogens with phytoestrogens.

ACS Style

Adrienn Balázs; Zelma Faisal; Rita Csepregi; Tamás Kőszegi; Balázs Kriszt; István Szabó; Miklós Poór. In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein. International Journal of Molecular Sciences 2021, 22, 6281 .

AMA Style

Adrienn Balázs, Zelma Faisal, Rita Csepregi, Tamás Kőszegi, Balázs Kriszt, István Szabó, Miklós Poór. In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein. International Journal of Molecular Sciences. 2021; 22 (12):6281.

Chicago/Turabian Style

Adrienn Balázs; Zelma Faisal; Rita Csepregi; Tamás Kőszegi; Balázs Kriszt; István Szabó; Miklós Poór. 2021. "In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein." International Journal of Molecular Sciences 22, no. 12: 6281.

Journal article
Published: 10 May 2021 in Molecules
Reads 0
Downloads 0

Melissopalynology, antioxidant capacity and mineral and toxic element contents were analyzed in eight types of Hungarian honeys. Based on color, two groups were distinguished: light honeys comprised acacia, amorpha, phacelia and linden honeys; while dark honeys included sunflower, chestnut, fennel and sage honeys, with 100 to 300 and 700 to 1500 mAU, respectively. The unifloral origin of each sample was supported using pollen analysis. The absorbance of honey correlated positively with antioxidant capacity determined by three different methods (TRC, DPPH, ORAC), and also with mineral content. The exception was the light amber linden honey with significantly higher K content and antiradical activity than other light honeys. The Mn, Zn and Fe contents were the highest in chestnut, sunflower and fennel honeys, respectively. The black meadow sage honey performed best regarding the content of other elements and antioxidant activity. The concentrations of several toxic elements were below the detection limit in the samples, indicating their good quality. The principal component analysis (PCA) revealed correlations between different antioxidant assays and minerals, and furthermore, confirmed the botanical authentication of the honeys based on the studied parameters. To our best knowledge, the present study is the first to provide a complex analysis of quality parameters of eight unifloral Hungarian honeys.

ACS Style

Alexandra Bodó; Lilla Radványi; Tamás Kőszegi; Rita Csepregi; Dávid Nagy; Ágnes Farkas; Marianna Kocsis. Quality Evaluation of Light- and Dark-Colored Hungarian Honeys, Focusing on Botanical Origin, Antioxidant Capacity and Mineral Content. Molecules 2021, 26, 2825 .

AMA Style

Alexandra Bodó, Lilla Radványi, Tamás Kőszegi, Rita Csepregi, Dávid Nagy, Ágnes Farkas, Marianna Kocsis. Quality Evaluation of Light- and Dark-Colored Hungarian Honeys, Focusing on Botanical Origin, Antioxidant Capacity and Mineral Content. Molecules. 2021; 26 (9):2825.

Chicago/Turabian Style

Alexandra Bodó; Lilla Radványi; Tamás Kőszegi; Rita Csepregi; Dávid Nagy; Ágnes Farkas; Marianna Kocsis. 2021. "Quality Evaluation of Light- and Dark-Colored Hungarian Honeys, Focusing on Botanical Origin, Antioxidant Capacity and Mineral Content." Molecules 26, no. 9: 2825.

Journal article
Published: 26 November 2020 in Molecules
Reads 0
Downloads 0

Onychomycosis is a disease that affects many adults, whose treatment includes both oral and topical therapies with low cure rates. The topical therapy is less effective but causes fewer side effects. This is why the development of an effective, easy to apply formulation for topical treatment is of high importance. We have used a nanotechnological approach to formulate Pickering emulsions (PEs) with well-defined properties to achieve site-specific delivery for antifungal drug combination of tioconazole and Melaleuca alternifolia essential oil. Silica nanoparticles with tailored size and partially hydrophobic surface have been synthesized and used for the stabilization of PEs. In vitro diffusion studies have been performed to evaluate the drug delivery properties of PEs. Ethanolic solution (ES) and conventional emulsions (CE) have been used as reference drug formulations. The examination of the antifungal effect of PEs has been performed on Candida albicans and Trichophyton rubrum as main pathogens. In vitro microbiological experimental results suggest that PEs are better candidates for onychomycosis topical treatment than CE or ES of the examined drugs. The used drugs have shown a significant synergistic effect, and the combination with an effective drug delivery system can result in a promising drug form for the topical treatment of onychomycosis.

ACS Style

Barbara Vörös-Horváth; Sourav Das; Ala’ Salem; Sándor Nagy; Andrea Böszörményi; Tamás Kőszegi; Szilárd Pál; Aleksandar Széchenyi. Formulation of Tioconazole and Melaleuca alternifolia Essential Oil Pickering Emulsions for Onychomycosis Topical Treatment. Molecules 2020, 25, 5544 .

AMA Style

Barbara Vörös-Horváth, Sourav Das, Ala’ Salem, Sándor Nagy, Andrea Böszörményi, Tamás Kőszegi, Szilárd Pál, Aleksandar Széchenyi. Formulation of Tioconazole and Melaleuca alternifolia Essential Oil Pickering Emulsions for Onychomycosis Topical Treatment. Molecules. 2020; 25 (23):5544.

Chicago/Turabian Style

Barbara Vörös-Horváth; Sourav Das; Ala’ Salem; Sándor Nagy; Andrea Böszörményi; Tamás Kőszegi; Szilárd Pál; Aleksandar Széchenyi. 2020. "Formulation of Tioconazole and Melaleuca alternifolia Essential Oil Pickering Emulsions for Onychomycosis Topical Treatment." Molecules 25, no. 23: 5544.

Journal article
Published: 13 June 2020 in Toxins
Reads 0
Downloads 0

Ochratoxins, patulin, deoxynivalenol, and T-2 toxin are mycotoxins, and common contaminants in food and drinks. Human serum albumin (HSA) forms complexes with certain mycotoxins. Since HSA can affect the toxicokinetics of bound ligand molecules, the potential interactions of ochratoxin B (OTB), ochratoxin C (OTC), patulin, deoxynivalenol, and T-2 toxin with HSA were examined, employing spectroscopic (fluorescence, UV, and circular dichroism) and ultrafiltration techniques. Furthermore, the influence of albumin on the cytotoxicity of these xenobiotics was also evaluated in cell experiments. Fluorescence studies showed the formation of highly stable OTB–HSA and OTC–HSA complexes. Furthermore, fluorescence quenching and circular dichroism measurements suggest weak or no interaction of patulin, deoxynivalenol, and T-2 toxin with HSA. In ultrafiltration studies, OTB and OTC strongly displaced the Sudlow’s site I ligand warfarin, while other mycotoxins tested did not affect either the albumin binding of warfarin or naproxen. The presence of HSA significantly decreased or even abolished the OTB- and OTC-induced cytotoxicity in cell experiments; however, the toxic impacts of patulin, deoxynivalenol, and T-2 toxin were not affected by HSA. In summary, the complex formation of OTB and OTC with albumin is relevant, whereas the interactions of patulin, deoxynivalenol, and T-2 toxin with HSA may have low toxicological importance.

ACS Style

Zelma Faisal; Virág Vörös; Eszter Fliszár-Nyúl; Beáta Lemli; Sándor Kunsági-Máté; Rita Csepregi; Tamás Kőszegi; Ferenc Zsila; Miklós Poór. Probing the Interactions of Ochratoxin B, Ochratoxin C, Patulin, Deoxynivalenol, and T-2 Toxin with Human Serum Albumin. Toxins 2020, 12, 1 .

AMA Style

Zelma Faisal, Virág Vörös, Eszter Fliszár-Nyúl, Beáta Lemli, Sándor Kunsági-Máté, Rita Csepregi, Tamás Kőszegi, Ferenc Zsila, Miklós Poór. Probing the Interactions of Ochratoxin B, Ochratoxin C, Patulin, Deoxynivalenol, and T-2 Toxin with Human Serum Albumin. Toxins. 2020; 12 (6):1.

Chicago/Turabian Style

Zelma Faisal; Virág Vörös; Eszter Fliszár-Nyúl; Beáta Lemli; Sándor Kunsági-Máté; Rita Csepregi; Tamás Kőszegi; Ferenc Zsila; Miklós Poór. 2020. "Probing the Interactions of Ochratoxin B, Ochratoxin C, Patulin, Deoxynivalenol, and T-2 Toxin with Human Serum Albumin." Toxins 12, no. 6: 1.

Journal article
Published: 21 May 2020 in Molecules
Reads 0
Downloads 0

The extreme lipophilicity of essential oils (EOs) impedes the measurement of their biological actions in an aqueous environment. We formulated oil in water type Pickering Artemisia annua EO nanoemulsions (AEP) with surface-modified Stöber silica nanoparticles (20 nm) as the stabilizing agent. The antimicrobial activity of AEP and its effects on mature Candida biofilms were compared with those of Tween 80 stabilized emulsion (AET) and ethanolic solution (AEE) of the Artemisia EO. The antimicrobial activity was evaluated by using the minimum inhibitory concentrations (MIC90) and minimum effective concentrations (MEC10) of the compounds. On planktonic bacterial and fungal cells beside growth inhibition, colony formation (CFU/mL), metabolic activity, viability, intracellular ATP/total protein (ATP/TP), along with reactive oxygen species (ROS) were also studied. Artemisia annua EO nanoemulsion (AEP) showed significantly higher antimicrobial activity than AET and AEE. Artemisia annua EO nanoemulsions (AEP) generated superoxide anion and peroxides-related oxidative stress, which might be the underlying mode of action of the Artemisia EO. Unilamellar liposomes, as a cellular model, were used to examine the delivery efficacy of the EO of our tested formulations. We could demonstrate higher effectiveness of AEP in the EO components’ donation compared to AET and AEE. Our data suggest the superiority of the AEP formulation against microbial infections.

ACS Style

Sourav Das; Barbara Vörös-Horváth; Tímea Bencsik; Giuseppe Micalizzi; Luigi Mondello; Györgyi Horváth; Tamás Kőszegi; Aleksandar Széchenyi. Antimicrobial Activity of Different Artemisia Essential Oil Formulations. Molecules 2020, 25, 2390 .

AMA Style

Sourav Das, Barbara Vörös-Horváth, Tímea Bencsik, Giuseppe Micalizzi, Luigi Mondello, Györgyi Horváth, Tamás Kőszegi, Aleksandar Széchenyi. Antimicrobial Activity of Different Artemisia Essential Oil Formulations. Molecules. 2020; 25 (10):2390.

Chicago/Turabian Style

Sourav Das; Barbara Vörös-Horváth; Tímea Bencsik; Giuseppe Micalizzi; Luigi Mondello; Györgyi Horváth; Tamás Kőszegi; Aleksandar Széchenyi. 2020. "Antimicrobial Activity of Different Artemisia Essential Oil Formulations." Molecules 25, no. 10: 2390.

Journal article
Published: 18 February 2020 in Antioxidants
Reads 0
Downloads 0

Medicinal plants are widely used in folk medicine but quite often their composition and biological effects are hardly known. Our study aimed to analyze the composition, cytotoxicity, antimicrobial, antioxidant activity and cellular migration effects of Anthyllis vulneraria, Fuchsia magellanica, Fuchsia triphylla and Lysimachia nummularia used in the Romanian ethnomedicine for wounds. Liquid chromatography with mass spectrometry (LC-MS/MS) was used to analyze 50% (v/v) ethanolic and aqueous extracts of the plants’ leaves. Antimicrobial activities were estimated with a standard microdilution method. The antioxidant properties were evaluated by validated chemical cell-free and biological cell-based assays. Cytotoxic effects were performed on mouse fibroblasts and human keratinocytes with a plate reader-based method assessing intracellular adenosine triphosphate (ATP), nucleic acid and protein contents and also by a flow cytometer-based assay detecting apoptotic–necrotic cell populations. Cell migration to cover cell-free areas was visualized by time-lapse phase-contrast microscopy using standard culture inserts. Fuchsia species showed the strongest cytotoxicity and the highest antioxidant and antimicrobial activity. However, their ethanolic extracts facilitated cell migration, most probably due to their various phenolic acid, flavonoid and anthocyanin derivatives. Our data might serve as a basis for further animal experiments to explore the complex action of Fuchsia species in wound healing assays.

ACS Style

Rita Csepregi; Viktória Temesfői; Sourav Das; Ágnes Alberti; Csenge Anna Tóth; Róbert Herczeg; Nóra Papp; Tamás Kőszegi. Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants. Antioxidants 2020, 9, 166 .

AMA Style

Rita Csepregi, Viktória Temesfői, Sourav Das, Ágnes Alberti, Csenge Anna Tóth, Róbert Herczeg, Nóra Papp, Tamás Kőszegi. Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants. Antioxidants. 2020; 9 (2):166.

Chicago/Turabian Style

Rita Csepregi; Viktória Temesfői; Sourav Das; Ágnes Alberti; Csenge Anna Tóth; Róbert Herczeg; Nóra Papp; Tamás Kőszegi. 2020. "Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants." Antioxidants 9, no. 2: 166.

Journal article
Published: 22 January 2020 in Molecules
Reads 0
Downloads 0

We investigated the antifungal activities of purified plant metabolites artemisinin (Ar) and scopoletin (Sc) including inhibition, effects on metabolic activities, viability, and oxidative stress on planktonic forms and on preformed biofilms of seven Candida species. The characteristic minimum inhibitory concentration (MIC90) of Ar and Sc against Candida species ranged from 21.83–142.1 µg/mL and 67.22–119.4 µg/mL, respectively. Drug concentrations causing ≈10% CFU decrease within 60 min of treatments were also determined (minimum effective concentration, MEC10) using 100-fold higher CFUs than in the case of MIC90 studies. Cytotoxic effects on planktonic and on mature biofilms of Candida species at MEC10 concentrations were further evaluated with fluorescent live/dead discrimination techniques. Candida glabrata, Candida guilliermondii, and Candida parapsilosis were the species most sensitive to Ar and Sc. Ar and Sc were also found to promote the accumulation of intracellular reactive oxygen species (ROS) by increasing oxidative stress at their respective MEC10 concentrations against the tested planktonic Candida species. Ar and Sc possess dose-dependent antifungal action but the underlying mechanism type (fungistatic and fungicidal) is not clear yet. Our data suggest that Ar and Sc found in herbal plants might have potential usage in the fight against Candida biofilms.

ACS Style

Sourav Das; Lilla Czuni; Viktória Báló; Gábor Papp; Zoltán Gazdag; Nóra Papp; Tamás Kőszegi; Papp. Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species. Molecules 2020, 25, 476 .

AMA Style

Sourav Das, Lilla Czuni, Viktória Báló, Gábor Papp, Zoltán Gazdag, Nóra Papp, Tamás Kőszegi, Papp. Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species. Molecules. 2020; 25 (3):476.

Chicago/Turabian Style

Sourav Das; Lilla Czuni; Viktória Báló; Gábor Papp; Zoltán Gazdag; Nóra Papp; Tamás Kőszegi; Papp. 2020. "Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species." Molecules 25, no. 3: 476.

Journal article
Published: 26 November 2019 in Molecules
Reads 0
Downloads 0

Essential oils (EOs) are highly lipophilic, which makes the measurement of their biological action difficult in an aqueous environment. We formulated a Pickering nanoemulsion of chamomile EO (CPe). Surface-modified Stöber silica nanoparticles (20 nm) were prepared and used as a stabilizing agent of CPe. The antimicrobial activity of CPe was compared with that of emulsion stabilized with Tween 80 (CT80) and ethanolic solution (CEt). The antimicrobial effects were assessed by their minimum inhibitory concentration (MIC90) and minimum effective (MEC10) concentrations. Besides growth inhibition (CFU/mL), the metabolic activity and viability of Gram-positive and Gram-negative bacteria as well as Candida species, in addition to the generation of oxygen free radical species (ROS), were studied. We followed the killing activity of CPe and analyzed the efficiency of the EO delivery for examined formulations by using unilamellar liposomes as a cellular model. CPe showed significantly higher antibacterial and antifungal activities than CT80 and CEt. Chamomile EOs generated superoxide anion and peroxide related oxidative stress which might be the major mode of action of Ch essential oil. We could also demonstrate that CPe was the most effective in donation of the active EO components when compared with CT80 and CEt. Our data suggest that CPe formulation is useful in the fight against microbial infections.

ACS Style

Sourav Das; Barbara Horváth; Silvija Šafranko; Stela Jokić; Aleksandar Széchenyi; Tamás Kőszegi. Antimicrobial Activity of Chamomile Essential Oil: Effect of Different Formulations. Molecules 2019, 24, 4321 .

AMA Style

Sourav Das, Barbara Horváth, Silvija Šafranko, Stela Jokić, Aleksandar Széchenyi, Tamás Kőszegi. Antimicrobial Activity of Chamomile Essential Oil: Effect of Different Formulations. Molecules. 2019; 24 (23):4321.

Chicago/Turabian Style

Sourav Das; Barbara Horváth; Silvija Šafranko; Stela Jokić; Aleksandar Széchenyi; Tamás Kőszegi. 2019. "Antimicrobial Activity of Chamomile Essential Oil: Effect of Different Formulations." Molecules 24, no. 23: 4321.

Clinical trial
Published: 07 November 2019 in PLOS ONE
Reads 0
Downloads 0

This study considered the effects of repeated bouts of short-term resistive exercise in old (age: 64.5±5.5 years; n = 10) and young men (age: 25.1±4.9 years; n = 10) who performed six knee extension exercise bouts over two weeks using various markers of exercise-induced muscle damage and electromyographic activity. We found that time-course changes in quadriceps isometric torque, creatine kinase activity, and muscle soreness in the two groups were similar. However, recovery in the acute torque deficit was mediated by more favourable electromyographic activity changes in the young group than in the older adults group. Muscle elastic energy storage and re-use assessed with dynamometry was selectively improved in the young group by the end of the protocol. Serum myoglobin concentration increased selectively in old group, and remained elevated with further bouts, suggesting higher sarcolemma vulnerability and less effective metabolic adaptation in the older adults, which, however, did not affect muscle contractility.

ACS Style

Zoltán Heckel; Tamás Atlasz; Éva Tékus; Tamás Kőszegi; József Laczkó; Márk Váczi. Monitoring exercise-induced muscle damage indicators and myoelectric activity during two weeks of knee extensor exercise training in young and old men. PLOS ONE 2019, 14, e0224866 .

AMA Style

Zoltán Heckel, Tamás Atlasz, Éva Tékus, Tamás Kőszegi, József Laczkó, Márk Váczi. Monitoring exercise-induced muscle damage indicators and myoelectric activity during two weeks of knee extensor exercise training in young and old men. PLOS ONE. 2019; 14 (11):e0224866.

Chicago/Turabian Style

Zoltán Heckel; Tamás Atlasz; Éva Tékus; Tamás Kőszegi; József Laczkó; Márk Váczi. 2019. "Monitoring exercise-induced muscle damage indicators and myoelectric activity during two weeks of knee extensor exercise training in young and old men." PLOS ONE 14, no. 11: e0224866.

Journal article
Published: 24 September 2019 in Chemosphere
Reads 0
Downloads 0

Zearalenone is a xenoestrogenic mycotoxin produced by Fusarium species. High exposure with zearalenone induces reproductive disorders worldwide. Cyclodextrins are ring-shaped host molecules built up from glucose units. The apolar cavity of cyclodextrins can entrap so-called guest molecules. The formation of highly stable host-guest type complexes with cyclodextrins can decrease the biological effect of the guest molecule. Therefore, cyclodextrins may be suitable to decrease the toxicity of some xenobiotics even after the exposure. In this study, the protective effect of beta-cyclodextrins against zearalenone-induced toxicity was investigated in HeLa cells and zebrafish embryos. Fluorescence spectroscopic studies demonstrated the formation of stable complexes of zearalenone with sulfobutyl-, methyl-, and succinyl-methyl-substituted beta-cyclodextrins at pH 7.4 (K = 1.4–4.7 × 104 L/mol). These chemically modified cyclodextrins considerably decreased or even abolished the zearalenone-induced loss of cell viability in HeLa cells and mortality in zebrafish embryos. Furthermore, the sublethal effects of zearalenone were also significantly alleviated by the co-treatment with beta-cyclodextrins. To test the estrogenic effect of the mycotoxin, a transgenic bioindicator zebrafish model (Tg(vtg1:mCherry)) was also applied. Our results suggest that the zearalenone-induced vitellogenin production is partly suppressed by the hepatotoxicity of zearalenone in zebrafish. This study demonstrates that the formation of stable zearalenone-cyclodextrin complexes can strongly decrease or even abolish the zearalenone-induced toxicity, both in vitro and in vivo. Therefore, cyclodextrins appear as promising new mycotoxin binders.

ACS Style

Zelma Faisal; Edina Garai; Rita Csepregi; Katalin Bakos; Eszter Fliszár-Nyúl; Lajos Szente; Adrienn Balázs; Mátyás Cserháti; Tamás Kőszegi; Béla Urbányi; Zsolt Csenki; Miklós Poór. Protective effects of beta-cyclodextrins vs. zearalenone-induced toxicity in HeLa cells and Tg(vtg1:mCherry) zebrafish embryos. Chemosphere 2019, 240, 124948 .

AMA Style

Zelma Faisal, Edina Garai, Rita Csepregi, Katalin Bakos, Eszter Fliszár-Nyúl, Lajos Szente, Adrienn Balázs, Mátyás Cserháti, Tamás Kőszegi, Béla Urbányi, Zsolt Csenki, Miklós Poór. Protective effects of beta-cyclodextrins vs. zearalenone-induced toxicity in HeLa cells and Tg(vtg1:mCherry) zebrafish embryos. Chemosphere. 2019; 240 ():124948.

Chicago/Turabian Style

Zelma Faisal; Edina Garai; Rita Csepregi; Katalin Bakos; Eszter Fliszár-Nyúl; Lajos Szente; Adrienn Balázs; Mátyás Cserháti; Tamás Kőszegi; Béla Urbányi; Zsolt Csenki; Miklós Poór. 2019. "Protective effects of beta-cyclodextrins vs. zearalenone-induced toxicity in HeLa cells and Tg(vtg1:mCherry) zebrafish embryos." Chemosphere 240, no. : 124948.

Original paper
Published: 19 November 2018 in European Journal of Clinical Investigation
Reads 0
Downloads 0

Background Laboratory markers are essential tools in the follow‐up of patients with Crohn's disease (CD). Our aim was to investigate urinary concentrations of orosomucoid in relation to the inflammatory activity of CD and to compare it with clinical indices and conventional laboratory parameters. Materials and methods Blood and urine samples of 86 patients (55 adults and 31 children) with CD and 68 healthy individuals (38 adults and 30 children) as controls were analyzed. Patients were categorized according to their clinical scores (Harvey‐Bradshaw Index (HBI) or Pediatric Crohn's Disease Activity Index (PCDAI)). Urinary orosomucoid (u‐ORM) was determined by automated immune turbidimetric assay and values were referred to urinary creatinine (u‐ORM/u‐CREAT, mg/mmol). Results U‐ORM/u‐CREAT values were 7 times higher in children with active CD (0.50 vs. 0.07 mg/mmol, p<0.001) and 2 times higher in adults (0.32 vs. 0.14 mg/mmol, p=0.01) compared with patients with inactive disease. U‐ORM/u‐CREAT showed good correlation with conventional inflammatory markers (hs‐CRP, serum ORM; p<0.01) and activity indices (HBI, p=0.018; PCDAI, p<0.001). U‐ORM/u‐CREAT had similar discriminative performance to hs‐CRP and serum ORM in the differentiation of active from inactive pediatric CD patients. Conclusions Our findings suggest that u‐ORM/u‐CREAT might serve as a valuable additional marker in the follow‐up of CD patients, especially in children for whom the non‐invasive sampling is a further advantage. This article is protected by copyright. All rights reserved.

ACS Style

Balázs Szirmay; Andras Tarnok; Patrícia Sarlós; Nóra Szigeti; Andrea Ludány; Péter Kustán; Zoltán Horváth‐Szalai; Attila Miseta; Tamás Kőszegi. Elevated urinary orosomucoid excretion as a novel biomarker in Crohn’s disease. European Journal of Clinical Investigation 2018, 49, e13054 .

AMA Style

Balázs Szirmay, Andras Tarnok, Patrícia Sarlós, Nóra Szigeti, Andrea Ludány, Péter Kustán, Zoltán Horváth‐Szalai, Attila Miseta, Tamás Kőszegi. Elevated urinary orosomucoid excretion as a novel biomarker in Crohn’s disease. European Journal of Clinical Investigation. 2018; 49 (3):e13054.

Chicago/Turabian Style

Balázs Szirmay; Andras Tarnok; Patrícia Sarlós; Nóra Szigeti; Andrea Ludány; Péter Kustán; Zoltán Horváth‐Szalai; Attila Miseta; Tamás Kőszegi. 2018. "Elevated urinary orosomucoid excretion as a novel biomarker in Crohn’s disease." European Journal of Clinical Investigation 49, no. 3: e13054.

Journal article
Published: 09 November 2018 in Food Chemistry
Reads 0
Downloads 0

Free essential oils and their active components have a low physiochemical stability and low aqueous solubility which limit their applications as food preservatives and in packaging industry. The aim of this study was to characterize the physicochemical properties, antioxidant activities and antimicrobial activity of randomly methylated β cyclodextrin (RAMEB) encapsulated thyme oil, lemon balm oil, lavender oil, peppermint oil and their active components that include thymol, citral, linalool, menthol and borneol. Inclusion complex formation of essential oils (EOs) and RAMEB were evaluated by several methods. Antioxidant capacities of RAMEB-EOs/components were reported to be more stable than free EOs/components (P < 0.05). Rapid SYBR green I/propidium iodide live/dead microbial cellular discrimination assay for Schizosaccharomyces pombe, Escherichia coli and Staphylococcus aureus showed similar results when compared with flow cytometry analysis (P < 0.01) suggesting that our novel microplate fluorescence method could be applied for the fast live/dead microbial discrimination in antimicrobial assays.

ACS Style

Sourav Das; Zoltán Gazdag; Lajos Szente; Mátyás Meggyes; Györgyi Horváth; Beáta Lemli; Sándor Kunsági-Máté; Mónika Kuzma; Tamás Kőszegi. Antioxidant and antimicrobial properties of randomly methylated β cyclodextrin – captured essential oils. Food Chemistry 2018, 278, 305 -313.

AMA Style

Sourav Das, Zoltán Gazdag, Lajos Szente, Mátyás Meggyes, Györgyi Horváth, Beáta Lemli, Sándor Kunsági-Máté, Mónika Kuzma, Tamás Kőszegi. Antioxidant and antimicrobial properties of randomly methylated β cyclodextrin – captured essential oils. Food Chemistry. 2018; 278 ():305-313.

Chicago/Turabian Style

Sourav Das; Zoltán Gazdag; Lajos Szente; Mátyás Meggyes; Györgyi Horváth; Beáta Lemli; Sándor Kunsági-Máté; Mónika Kuzma; Tamás Kőszegi. 2018. "Antioxidant and antimicrobial properties of randomly methylated β cyclodextrin – captured essential oils." Food Chemistry 278, no. : 305-313.

Validation study
Published: 08 September 2018 in International Journal of Molecular Sciences
Reads 0
Downloads 0

A fluorescence-based enzymatic microplate intracellular glucose assay was designed and fully validated. The method was tested in a hepatocellular cancer cell line (HepG2). Our novel one-step extraction reagent gave stable cell lysates for glucose, adenosine triphosphate (ATP), and total protein determination from the same sample. Limit of detection for glucose was 0.13 µM (26 pmol/well), which is superior to commercially available glucose assays. Both intra- and interday assay imprecision in HepG2 cultures were less than 12% coefficient of variance (CV). In cell lysates spiked with glucose, recovery at two levels varied between 83.70% and 91.81%, and both linearity and stability were acceptable. HepG2 cells treated with agents affecting glucose uptake/metabolism (phloretin, quercetin, quercetin-3′-sulfate, NaF, 3-bromopyruvate, NaN3, oligomycin A, ochratoxin A, cytochalasin B, and anti-GLUT1 antibody) showed dose-dependent changes in glucose and ATP levels without total protein (cell) loss. Finally, we performed flow cytometric glucose uptake measurement in the treated cells using 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose fluorescent glucose analog. Glucose uptake did not always mirror the intracellular glucose levels, which most likely reflects the differences between the two methodologies. However, interpreting data obtained by both methods and taking ATP/protein levels at the same time, one can get information on the mode of action of the compounds.

ACS Style

Rita Csepregi; Viktória Temesfői; Nikolett Sali; Miklós Poór; Paul W. Needs; Paul A. Kroon; Tamás Kőszegi. A One-Step Extraction and Luminescence Assay for Quantifying Glucose and ATP Levels in Cultured HepG2 Cells. International Journal of Molecular Sciences 2018, 19, 2670 .

AMA Style

Rita Csepregi, Viktória Temesfői, Nikolett Sali, Miklós Poór, Paul W. Needs, Paul A. Kroon, Tamás Kőszegi. A One-Step Extraction and Luminescence Assay for Quantifying Glucose and ATP Levels in Cultured HepG2 Cells. International Journal of Molecular Sciences. 2018; 19 (9):2670.

Chicago/Turabian Style

Rita Csepregi; Viktória Temesfői; Nikolett Sali; Miklós Poór; Paul W. Needs; Paul A. Kroon; Tamás Kőszegi. 2018. "A One-Step Extraction and Luminescence Assay for Quantifying Glucose and ATP Levels in Cultured HepG2 Cells." International Journal of Molecular Sciences 19, no. 9: 2670.

Journal article
Published: 01 September 2018 in Toxins
Reads 0
Downloads 0

Ochratoxin A (OTA) is a nephrotoxic mycotoxin. Roasting of OTA-contaminated coffee results in the formation of 2′R-ochratoxin A (2′R-OTA), which appears in the blood of coffee drinkers. Human serum albumin (HSA) binds 2′R-OTA (and OTA) with high affinity; therefore, albumin may influence the tissue uptake and elimination of ochratoxins. We aimed to investigate the binding site of 2′R-OTA (verses OTA) in HSA and the displacing effects of site markers to explore which molecules can interfere with its albumin-binding. Affinity of 2′R-OTA toward albumins from various species (human, bovine, porcine and rat) was tested to evaluate the interspecies differences regarding 2′R-OTA-albumin interaction. Thermodynamic studies were performed to give a deeper insight into the molecular background of the complex formation. Besides fluorescence spectroscopic and modeling studies, effects of HSA, and fetal bovine serum on the cytotoxicity of 2′R-OTA and OTA were tested in MDCK kidney cell line in order to demonstrate the influence of albumin-binding on the cellular uptake of ochratoxins. Site markers displaced more effectively 2′R-OTA than OTA from HSA. Fluorescence and binding constants of 2′R-OTA-albumin and OTA-albumin complexes showed different tendencies. Albumin significantly decreased the cytotoxicity of ochratoxins. 2′R-OTA, even at sub-toxic concentrations, increased the toxic action of OTA.

ACS Style

Zelma Faisal; Diána Derdák; Beáta Lemli; Sándor Kunsági-Máté; Mónika Bálint; Csaba Hetényi; Rita Csepregi; Tamás Kőszegi; Franziska Sueck; Benedikt Cramer; Hans-Ulrich Humpf; Miklós Poór. Interaction of 2′R-ochratoxin A with Serum Albumins: Binding Site, Effects of Site Markers, Thermodynamics, Species Differences of Albumin-binding, and Influence of Albumin on Its Toxicity in MDCK Cells. Toxins 2018, 10, 353 .

AMA Style

Zelma Faisal, Diána Derdák, Beáta Lemli, Sándor Kunsági-Máté, Mónika Bálint, Csaba Hetényi, Rita Csepregi, Tamás Kőszegi, Franziska Sueck, Benedikt Cramer, Hans-Ulrich Humpf, Miklós Poór. Interaction of 2′R-ochratoxin A with Serum Albumins: Binding Site, Effects of Site Markers, Thermodynamics, Species Differences of Albumin-binding, and Influence of Albumin on Its Toxicity in MDCK Cells. Toxins. 2018; 10 (9):353.

Chicago/Turabian Style

Zelma Faisal; Diána Derdák; Beáta Lemli; Sándor Kunsági-Máté; Mónika Bálint; Csaba Hetényi; Rita Csepregi; Tamás Kőszegi; Franziska Sueck; Benedikt Cramer; Hans-Ulrich Humpf; Miklós Poór. 2018. "Interaction of 2′R-ochratoxin A with Serum Albumins: Binding Site, Effects of Site Markers, Thermodynamics, Species Differences of Albumin-binding, and Influence of Albumin on Its Toxicity in MDCK Cells." Toxins 10, no. 9: 353.

Journal article
Published: 28 June 2018 in Molecules
Reads 0
Downloads 0

Green fluorescent protein (GFP) is considered to be suitable for cell viability testing. In our study, GFP transfected A549 lung carcinoma cell line was treated with sodium fluoride (NaF), cycloheximide (CHX) and ochratoxin A (OTA). GFP fluorescence, intracellular ATP, nucleic acid and protein contents were quantified by a luminescence microplate assay developed in our laboratory. Flow cytometry was used to confirm the findings and to assess the intensity of GFP during different types of cell death. A 24 h NaF and CHX exposure caused a dramatic decrease in ATP contents (p < 0.05) compared with those of the controls. GFP fluorescence of the cells was in close correlation with total protein; however, GFP/ATP increased at NaF and decreased at CHX treatments (p < 0.05). ATP/protein and ATP/propidium iodide (PI) were largely decreased at NaF exposure in a dose-dependent manner (p < 0.05), while CHX and OTA showed markedly fewer effects. Both treatments caused apoptosis/necrosis at different rates. NaF induced mainly late apoptosis while OTA, mainly apoptosis. CHX effects varied by the incubation time with 100-fold elevation in late apoptotic cells at 24 h treatment. GFP intensity did not show a significant difference between live and apoptotic populations. Our results suggest when using GFP, a multiparametric assay is necessary for more precise interpretation of cell viability.

ACS Style

Rita Csepregi; Viktória Temesfői; Miklós Poór; Zsuzsanna Faust; Tamás Kõszegi. Green Fluorescent Protein-Based Viability Assay in a Multiparametric Configuration. Molecules 2018, 23, 1575 .

AMA Style

Rita Csepregi, Viktória Temesfői, Miklós Poór, Zsuzsanna Faust, Tamás Kõszegi. Green Fluorescent Protein-Based Viability Assay in a Multiparametric Configuration. Molecules. 2018; 23 (7):1575.

Chicago/Turabian Style

Rita Csepregi; Viktória Temesfői; Miklós Poór; Zsuzsanna Faust; Tamás Kõszegi. 2018. "Green Fluorescent Protein-Based Viability Assay in a Multiparametric Configuration." Molecules 23, no. 7: 1575.

Journal article
Published: 01 March 2018 in Bioanalysis
Reads 0
Downloads 0

Aim: There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. Materials & methods: A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed. Results: Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes. Conclusion: We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.

ACS Style

Balázs Szirmay; Peter Kustan; Zoltán Horváth-Szalai; Andrea Ludány; Ágnes Lakatos; Diána Mühl; István Wittmann; Attila Miseta; Gábor L. Kovács; Tamás Kőszegi. Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations. Bioanalysis 2018, 10, 377 -384.

AMA Style

Balázs Szirmay, Peter Kustan, Zoltán Horváth-Szalai, Andrea Ludány, Ágnes Lakatos, Diána Mühl, István Wittmann, Attila Miseta, Gábor L. Kovács, Tamás Kőszegi. Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations. Bioanalysis. 2018; 10 (6):377-384.

Chicago/Turabian Style

Balázs Szirmay; Peter Kustan; Zoltán Horváth-Szalai; Andrea Ludány; Ágnes Lakatos; Diána Mühl; István Wittmann; Attila Miseta; Gábor L. Kovács; Tamás Kőszegi. 2018. "Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations." Bioanalysis 10, no. 6: 377-384.

Journal article
Published: 10 February 2018 in Molecules
Reads 0
Downloads 0

Resazurin (or Alamar Blue) is a poorly fluorescent dye. During the cellular reduction of resazurin, its highly fluorescent product resorufin is formed. Resazurin assay is a commonly applied method to investigate viability of bacterial and mammalian cells. In this study, the interaction of resazurin and resorufin with β-cyclodextrins was investigated employing spectroscopic and molecular modeling studies. Furthermore, the influence of β-cyclodextrins on resazurin-based cell viability assay was also tested. Both resazurin and resorufin form stable complexes with the examined β-cyclodextrins (2.0–3.1 × 103 and 1.3–1.8 × 103 L/mol were determined as binding constants, respectively). Cells were incubated for 30 and 120 min and treated with resazurin and/or β-cyclodextrins. Our results suggest that cyclodextrins are able to interfere with the resazurin-based cell viability assay that presumably results from the following mechanisms: (1) inhibition of the cellular uptake of resazurin and (2) enhancement of the fluorescence signal of the formed resorufin.

ACS Style

Rita Csepregi; Beáta Lemli; Sándor Kunsági-Máté; Lajos Szente; Tamás Kõszegi; Balázs Németi; Miklós Poór. Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay? Molecules 2018, 23, 382 .

AMA Style

Rita Csepregi, Beáta Lemli, Sándor Kunsági-Máté, Lajos Szente, Tamás Kõszegi, Balázs Németi, Miklós Poór. Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay? Molecules. 2018; 23 (2):382.

Chicago/Turabian Style

Rita Csepregi; Beáta Lemli; Sándor Kunsági-Máté; Lajos Szente; Tamás Kõszegi; Balázs Németi; Miklós Poór. 2018. "Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay?" Molecules 23, no. 2: 382.

Journal article
Published: 10 January 2018 in Clinical Chemistry and Laboratory Medicine (CCLM)
Reads 0
Downloads 0

Background: Simultaneous determination of the two main actin scavenger proteins in sepsis has not been investigated until now. In our pilot study, we elucidated the predictive values of Gc globulin and gelsolin (GSN) in sepsis by comparing them to classic laboratory and clinical parameters. Methods: A 5-day follow-up was performed, including 46 septic patients, 28 non-septic patients and 35 outpatients as controls. Serum Gc globulin and GSN levels were determined by automated immune turbidimetric assay on a Cobas 8000/c502 analyzer. Patients were retrospectively categorized according to the sepsis-3 definitions, and 14-day mortality was also investigated. Results: First-day GSN also differentiated sepsis from non-sepsis (AUC: 0.88) similarly to C-reactive protein (AUC: 0.80) but was slightly inferior to procalcitonin (PCT) (AUC: 0.98) with a cutoff value of GSN at 22.29 mg/L (sensitivity: 83.3%; specificity: 86.2%). Only first-day SOFA scores (0.88) and GSN (0.71) distinguished septic survivors from non-survivors, whereas lactate (0.99), Gc globulin (0.76) and mean arterial pressure (MAP) (0.74) discriminated septic shock from sepsis. Logistic regression analyses revealed SOFA scores and GSN being significant factors regarding 14-day mortality. First-day GSN levels were higher (p Conclusions: Both serum GSN and Gc globulin may have predictive values in sepsis. Considering the small sample size of our study, further measurements are needed to evaluate our results. Measurement of Gc globulin and GSN maybe useful in assessment of sepsis severity and in therapeutic decision-making.

ACS Style

Zoltán Horváth-Szalai; Péter Kustán; Balázs Szirmay; Ágnes Lakatos; Per Hjort Christensen; Tamás Huber; Beáta Bugyi; Diána Mühl; Andrea Ludány; Attila Miseta; Gábor L. Kovács; Tamás Kőszegi. Predictive value of serum gelsolin and Gc globulin in sepsis – a pilot study. Clinical Chemistry and Laboratory Medicine (CCLM) 2018, 56, 1373 -1382.

AMA Style

Zoltán Horváth-Szalai, Péter Kustán, Balázs Szirmay, Ágnes Lakatos, Per Hjort Christensen, Tamás Huber, Beáta Bugyi, Diána Mühl, Andrea Ludány, Attila Miseta, Gábor L. Kovács, Tamás Kőszegi. Predictive value of serum gelsolin and Gc globulin in sepsis – a pilot study. Clinical Chemistry and Laboratory Medicine (CCLM). 2018; 56 (8):1373-1382.

Chicago/Turabian Style

Zoltán Horváth-Szalai; Péter Kustán; Balázs Szirmay; Ágnes Lakatos; Per Hjort Christensen; Tamás Huber; Beáta Bugyi; Diána Mühl; Andrea Ludány; Attila Miseta; Gábor L. Kovács; Tamás Kőszegi. 2018. "Predictive value of serum gelsolin and Gc globulin in sepsis – a pilot study." Clinical Chemistry and Laboratory Medicine (CCLM) 56, no. 8: 1373-1382.

Journal article
Published: 25 October 2017 in Toxins
Reads 0
Downloads 0

Aflatoxins are widely spread mycotoxins produced mainly by Aspergillus species. Consumption of aflatoxin-contaminated foods and drinks causes serious health risks for people worldwide. It is well-known that the reactive epoxide metabolite of aflatoxin B1 (AFB1) forms covalent adducts with serum albumin. However, non-covalent interactions of aflatoxins with human serum albumin (HSA) are poorly characterized. Thus, in this study the complex formation of aflatoxins was examined with HSA applying spectroscopic and molecular modelling studies. Our results demonstrate that aflatoxins form stable complexes with HSA as reflected by binding constants between 2.1 × 104 and 4.5 × 104 dm3/mol. A binding free energy value of −26.90 kJ mol−1 suggests a spontaneous binding process between AFB1 and HSA at room-temperature, while the positive entropy change of 55.1 JK−1 mol−1 indicates a partial decomposition of the solvation shells of the interacting molecules. Modeling studies and investigations with site markers suggest that Sudlow’s Site I of subdomain IIA is the high affinity binding site of aflatoxins on HSA. Interaction of AFB1 with bovine, porcine, and rat serum albumins was also investigated. Similar stabilities of the examined AFB1-albumin complexes were observed suggesting the low species differences of the albumin-binding of aflatoxins.

ACS Style

Miklós Poór; Mónika Bálint; Csaba Hetényi; Beatrix Gődér; Sándor Kunsági-Máté; Tamás Kőszegi; Beáta Lemli. Investigation of Non-Covalent Interactions of Aflatoxins (B1, B2, G1, G2, and M1) with Serum Albumin. Toxins 2017, 9, 339 .

AMA Style

Miklós Poór, Mónika Bálint, Csaba Hetényi, Beatrix Gődér, Sándor Kunsági-Máté, Tamás Kőszegi, Beáta Lemli. Investigation of Non-Covalent Interactions of Aflatoxins (B1, B2, G1, G2, and M1) with Serum Albumin. Toxins. 2017; 9 (11):339.

Chicago/Turabian Style

Miklós Poór; Mónika Bálint; Csaba Hetényi; Beatrix Gődér; Sándor Kunsági-Máté; Tamás Kőszegi; Beáta Lemli. 2017. "Investigation of Non-Covalent Interactions of Aflatoxins (B1, B2, G1, G2, and M1) with Serum Albumin." Toxins 9, no. 11: 339.

Journal article
Published: 28 September 2017 in Journal of Pharmacological and Toxicological Methods
Reads 0
Downloads 0

Reactive oxygen species (ROS) are normal metabolic products of living cells. However, a decrease of the defense mechanisms against the effects of ROS or increased ROS production maybe one important causative factor of cellular damage. A non-enzymatic scavenger system is considered to be responsible for the maintenance of total antioxidant capacity (TAC) as a protection against oxidative injuries that exist in all higher plants and in mammals as well. In our work, we optimized and validated a luminol-peroxidase-4-iodophenol-H2O2 enhanced chemiluminescence-based (ECL) TAC measurement technique. BSA was applied in the reagent to prevent peroxidase from auto-oxidation. The ECL method was suitable for plant extracts and for human blood serum as well. Our TAC technique was adapted to microplates and compared to ORAC assay using plant extracts. The ECL method is fast (10 min) with an interassay precision of < 10% as CV. TAC values of ethanolic extracts of 10 plant species did correlate (ECL vs ORAC assay data: r = 0.84, 95% confidence interval, CI = 0.78–0.89, P < 0.001) but with systematic bias. Analysis of serum samples obtained from septic and control patients showed significantly higher TAC values in the patient group compared to those of controls (p < 0.01). Moreover, we could discriminate between surviving and non-surviving patients, based on their TAC values (p < 0.01). Pearson's statistics showed the strongest positive correlation with serum uric acid (r = 0.73). Besides the routine laboratory parameters, our novel TAC method might give complementary information on the severity of systemic inflammation.

ACS Style

Tamás Kőszegi; Nikolett Sali; Maja Raknić; Zoltán Horváth-Szalai; Rita Csepregi; Marijana Zovko Končić; Nóra Papp; Miklós Poór. A novel luminol-based enhanced chemiluminescence antioxidant capacity microplate assay for use in different biological matrices. Journal of Pharmacological and Toxicological Methods 2017, 88, 153 -159.

AMA Style

Tamás Kőszegi, Nikolett Sali, Maja Raknić, Zoltán Horváth-Szalai, Rita Csepregi, Marijana Zovko Končić, Nóra Papp, Miklós Poór. A novel luminol-based enhanced chemiluminescence antioxidant capacity microplate assay for use in different biological matrices. Journal of Pharmacological and Toxicological Methods. 2017; 88 ():153-159.

Chicago/Turabian Style

Tamás Kőszegi; Nikolett Sali; Maja Raknić; Zoltán Horváth-Szalai; Rita Csepregi; Marijana Zovko Končić; Nóra Papp; Miklós Poór. 2017. "A novel luminol-based enhanced chemiluminescence antioxidant capacity microplate assay for use in different biological matrices." Journal of Pharmacological and Toxicological Methods 88, no. : 153-159.