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The nomadic pastoral indigenous communities of the Ladakhi people share roots with Tibetan culture in terms of food, clothing, religion, festivals, and habits, and rely widely on plant resources for survival and livelihood. This survey was conducted during 2019–2021 to document the indigenous knowledge about plant resources of the Balti, Beda, and Brokpa communities of the Ladakh region, trans-Himalayas. Open- and close-ended semi-structured interviews (N = 184) and group discussions (N = 17) were used to collect the data. Quantitative data was further analyzed using various statistical tools. A total of 105 plant species belonging to 82 genera and 39 families were used as medicine, fuel wood, fragrance, oil, food, flavor, fodder, decoration, and dye. Among these, medicinal use was most prevalent, with 70% of use reports, followed by fodder and fuel wood. Leaves (27%) were the most preferred plant part used, followed by roots and flowers. The principal component analysis revealed five clusters of ethnobotanical usage, i.e., food, medicine, fuel wood, fodder, and fragrance, oil, dye, and flavor. The maximum number of plant species used was reported by the Brokpa, while the Beda reported the minimum number of plant species uses. Delphinium brunonianum, Waldheimia tomentosa, and Juniperus indica played a significant role in the cultural and religious ritual aspects, whereas Allium przewalskianum, Waldheimia tomentosa, Juniperus indica, and Hippophae rhamnoides were commonly used as a livelihood source among Ladakhi communities. The local people collected most plants (65%) for self-consumption, while the rest (35%) were sold in markets as a source of income. The sustainable utilization and management of plant resources by local people is a strategy to boost livelihoods and food security and alleviate poverty.
Shiekh Marifatul Haq; Umer Yaqoob; Eduardo Soares Calixto; Inayat Ur Rahman; Abeer Hashem; Elsayed Fathi Abd_Allah; Maha Abdullah Alakeel; Abdulaziz A. Alqarawi; Mohnad Abdalla; Musheerul Hassan; Rainer W. Bussmann; Arshad Mehmood Abbasi; Sami Ur Rahman; Farhana Ijaz. Plant Resources Utilization among Different Ethnic Groups of Ladakh in Trans-Himalayan Region. Biology 2021, 10, 827 .
AMA StyleShiekh Marifatul Haq, Umer Yaqoob, Eduardo Soares Calixto, Inayat Ur Rahman, Abeer Hashem, Elsayed Fathi Abd_Allah, Maha Abdullah Alakeel, Abdulaziz A. Alqarawi, Mohnad Abdalla, Musheerul Hassan, Rainer W. Bussmann, Arshad Mehmood Abbasi, Sami Ur Rahman, Farhana Ijaz. Plant Resources Utilization among Different Ethnic Groups of Ladakh in Trans-Himalayan Region. Biology. 2021; 10 (9):827.
Chicago/Turabian StyleShiekh Marifatul Haq; Umer Yaqoob; Eduardo Soares Calixto; Inayat Ur Rahman; Abeer Hashem; Elsayed Fathi Abd_Allah; Maha Abdullah Alakeel; Abdulaziz A. Alqarawi; Mohnad Abdalla; Musheerul Hassan; Rainer W. Bussmann; Arshad Mehmood Abbasi; Sami Ur Rahman; Farhana Ijaz. 2021. "Plant Resources Utilization among Different Ethnic Groups of Ladakh in Trans-Himalayan Region." Biology 10, no. 9: 827.
Being a global concern, the current scenario of food chain contamination is one of the major threats to human health. Unrestrained human population, industrialization, urbanization, and extensive use of agrochemicals are among the major causes of heavy metals (HMs) contamination in the food chain. The present study was intended to quantify some HMs and to evaluate their sources and health risks in different varieties of onion using standard analytical approaches. Overall, nine HMs were quantified in the bulbs and leaves of nine varieties of onion. Ascending order of HMs’ concentration was as follow: Fe > Zn > Mn > Co > Pb > Cu > Cr > Cd > Li (bulbs) and Fe > Mn > Zn > Cu > Cr > Pb > Co > Li > Cd (leaves). Most of the measured levels were found to be relatively higher in the leaves than bulbs. Red Flame (V4), Super Sarhad (V3), Red Orb (V2), White Pearl (V7) and Zeus (V9) varieties showed elevated levels of Cu, Fe, and Zn; Cr and Mn; Co and Pb; Cd and Li, respectively in the bulbs. Likewise, Red Orb (V2) and White Pearl (V7) revealed elevated levels of the metals, while Golden Orb (V6) exhibited relatively lower metal levels. Highly significant positive correlations (≥90% at p ≤ 0.01) were found between Zn-Cu and Zn-Fe (in bulbs) and Cu-Co (in leaves), indicating their common origin. Principle component analysis indicated substantial anthropogenic contamination of the HMs in onion. In addition, hierarchal clustering revealed associations of different varieties based on similarities in the metal levels. The calculated values of health risk index (HRI), target hazard quotient (THQ), hazard index (HI), and target cancer risk (TCR) in the bulbs and leaves of all varieties were within the safe limit for both adults and children. Therefore, human consumption of these onion varieties is considered safe with respect to the HMs. However, currently used remediation approaches should focus on the reduction of health risks associated with HMs contamination in the food crops.
Nusrat Bibi; Munir H. Shah; Nadeem Khan; Qaisar Mahmood; Ali Abdullah Aldosari; Arshad Mehmood Abbasi. Analysis and health risk assessment of heavy metals in some onion varieties. Arabian Journal of Chemistry 2021, 14, 103364 .
AMA StyleNusrat Bibi, Munir H. Shah, Nadeem Khan, Qaisar Mahmood, Ali Abdullah Aldosari, Arshad Mehmood Abbasi. Analysis and health risk assessment of heavy metals in some onion varieties. Arabian Journal of Chemistry. 2021; 14 (10):103364.
Chicago/Turabian StyleNusrat Bibi; Munir H. Shah; Nadeem Khan; Qaisar Mahmood; Ali Abdullah Aldosari; Arshad Mehmood Abbasi. 2021. "Analysis and health risk assessment of heavy metals in some onion varieties." Arabian Journal of Chemistry 14, no. 10: 103364.
The strong association between POPs and breast cancer in humans has been suggested in various epidemiological studies. However, the interaction of POPs with the ERα protein of breast cancer, and identification of natural and synthetic compounds to inhibit this interaction, is mysterious yet. Consequently, the present study aimed to explore the interaction between POPs and ERα using the molecular operating environment (MOE) tool and to identify natural and synthetic compounds to inhibit this association through a cluster-based approach. To validate whether our approach could distinguish between active and inactive compounds, a virtual screen (VS) was performed using actives (627 compounds) as positive control and decoys (20,818 compounds) as a negative dataset obtained from DUD-E. Comparatively, short-chain chlorinated paraffins (SCCPs), hexabromocyclododecane (HBCD), and perfluorooctanesulfonyl fluoride (PFOSF) depicted strong interactions with the ERα protein based on the lowest-scoring values of −31.946, −18.916, −17.581 kcal/mol, respectively. Out of 7856 retrieved natural and synthetic compounds, sixty were selected on modularity bases and subsequently docked with ERα. Based on the lowest-scoring values, ZINC08441573, ZINC00664754, ZINC00702695, ZINC00627464, and ZINC08440501 (synthetic compounds), and capsaicin, flavopiridol tectorgenin, and ellagic acid (natural compounds) showed incredible interactions with the active sites of ERα, even more convening and resilient than standard breast cancer drugs Tamoxifen, Arimidex and Letrozole. Our findings confirm the role of POPs in breast cancer progression and suggest that natural and synthetic compounds with high binding affinity could be more efficient and appropriate candidates to treat breast cancer after validation through in vitro and in vivo studies.
Bibi Zainab; Zainab Ayaz; Umer Rashid; Dunia Al Farraj; Roua Alkufeidy; Fatmah AlQahtany; Reem Aljowaie; Arshad Abbasi. Role of Persistent Organic Pollutants in Breast Cancer Progression and Identification of Estrogen Receptor Alpha Inhibitors Using In-Silico Mining and Drug-Drug Interaction Network Approaches. Biology 2021, 10, 681 .
AMA StyleBibi Zainab, Zainab Ayaz, Umer Rashid, Dunia Al Farraj, Roua Alkufeidy, Fatmah AlQahtany, Reem Aljowaie, Arshad Abbasi. Role of Persistent Organic Pollutants in Breast Cancer Progression and Identification of Estrogen Receptor Alpha Inhibitors Using In-Silico Mining and Drug-Drug Interaction Network Approaches. Biology. 2021; 10 (7):681.
Chicago/Turabian StyleBibi Zainab; Zainab Ayaz; Umer Rashid; Dunia Al Farraj; Roua Alkufeidy; Fatmah AlQahtany; Reem Aljowaie; Arshad Abbasi. 2021. "Role of Persistent Organic Pollutants in Breast Cancer Progression and Identification of Estrogen Receptor Alpha Inhibitors Using In-Silico Mining and Drug-Drug Interaction Network Approaches." Biology 10, no. 7: 681.
Traditional ecological knowledge, linguistic, and sociocultural perspectives are key contributors to environmental sustainability. Therefore, it is essential to identify and preserve this biocultural heritage, especially that of indigenous communities and minorities. We conducted an ethnobotanical survey to document the plant species used by the Balti and Shina communities living in the buffer zone of Deosai National Park (DNP), western Himalayas, Pakistan. A combination of random and purposive sampling techniques was adapted, targeting middle- and old-aged informants. A total of 46 semi-structured interviews were conducted and the gathered data were evaluated using relative frequency of citation (RFC) and through comparison with the ethnomedicinal literature. In total, 47 medicinal plant species belonging to 42 genera and 23 families were recorded. Baltis and Shinas cited 42 and 38 plant species, respectively, that were used to treat various diseases. About 60% of species were common among both communities, but 27.7% and 12.8% were exclusive to Baltis and Shinas, respectively. Considerable heterogeneity was noted in vernacular names, plant part(s) used, preparation, and administration. Ribes alpestre, Aconitum violaceum, Delphinium brunonianum, Thymus linearis, and Swertia petiolata were highly utilized species having RFCs > 50. In addition, 46% of medicinal uses, specifically that of Allardia tomentosa, A. tridactylites, Jurinea dolomiaea, and Gallium boreale, were reported for the first time from the region. Cross-cultural analysis revealed sociocultural gaps between both groups. Relatively, Baltis retained more ethnomedicinal knowledge and their traditional medicinal system is more closely associated with traditional Tibetan medicine. Generally, Balti and Shina communities retain substantial biocultural and ethnological diversity, which has been reflected in the present study. Our findings underline the importance and need for sustainable utilization of natural resources, specifically the plant species of this region. However, an in-depth ethnobotanical investigation may underpin the holistic comparative medical ethnobotany of the entire region.
Zaheer Abbas; Shazia Kousar; Muhammad Aziz; Andrea Pieroni; Ali Aldosari; Rainer Bussmann; Ghulam Raza; Arshad Abbasi. Comparative Assessment of Medicinal Plant Utilization among Balti and Shina Communities in the Periphery of Deosai National Park, Pakistan. Biology 2021, 10, 434 .
AMA StyleZaheer Abbas, Shazia Kousar, Muhammad Aziz, Andrea Pieroni, Ali Aldosari, Rainer Bussmann, Ghulam Raza, Arshad Abbasi. Comparative Assessment of Medicinal Plant Utilization among Balti and Shina Communities in the Periphery of Deosai National Park, Pakistan. Biology. 2021; 10 (5):434.
Chicago/Turabian StyleZaheer Abbas; Shazia Kousar; Muhammad Aziz; Andrea Pieroni; Ali Aldosari; Rainer Bussmann; Ghulam Raza; Arshad Abbasi. 2021. "Comparative Assessment of Medicinal Plant Utilization among Balti and Shina Communities in the Periphery of Deosai National Park, Pakistan." Biology 10, no. 5: 434.
Natural antioxidants possess health beneficial properties and are being substantially used in foods, cosmetics and pharmaceutical products. Polyphenolics content, antioxidant activity and HepG2 liver cancer cells’ inhibition potential were determined and compared in the fruit of apricot cultivars collected from lower Himalayan region of Pakistan using standard analytical procedures. Comparatively, fruit of AP3 cultivar had highest levels of total phenolics and total flavonoids contents (220.5 ± 15.9 mgGAE/100 g and 382.0 ± 18.0 mgQE/100 g, respectively) on fresh weight basis. Fruit of the same cultivar had maximum DPPH scvenging potential (94.24 ± 4.55%), oxygen radicals absorbance capacity at 4.71 ± 0.12 mMolTE/100 g and inhibition of HepG2 cancer cells (45.80 ± 4.01 µMolQE/100 g). Though, fruit of AP4 cultivar was rich in vanillic acid, caffeic acid, vanillin, epi-catechin and quercetin, but had relatively low antioxidant and anticancer potential. Disparities in the phytochemical composition and properties of apricot fruit are attributed to genetic differences and growing conditions. Total phenolics, total flavonoids and caffic acid depicted highly significant positive association (˃90%) with ORAC, CAA and FRAP. Our findings suggest that being a rich source of health beneficial secondary metabolites, apricot fruit of Himalayan region of Pakistan could contribute considerably in socio-economic development of the local communities and as a functional food in regional and global markets.
Iqra Saeed; Xinbo Guo; Muhammad Azeem; Mohamed Soliman Elshikh; Bibi Zainab; Zainab Ayaz; Lijun You; Mona S. Alwahibi; Arshad Mehmood Abbasi. Comparative assessment of polyphenolics’ content, free radicals’ scavenging and cellular antioxidant potential in apricot fruit. Journal of King Saud University - Science 2021, 33, 101459 .
AMA StyleIqra Saeed, Xinbo Guo, Muhammad Azeem, Mohamed Soliman Elshikh, Bibi Zainab, Zainab Ayaz, Lijun You, Mona S. Alwahibi, Arshad Mehmood Abbasi. Comparative assessment of polyphenolics’ content, free radicals’ scavenging and cellular antioxidant potential in apricot fruit. Journal of King Saud University - Science. 2021; 33 (5):101459.
Chicago/Turabian StyleIqra Saeed; Xinbo Guo; Muhammad Azeem; Mohamed Soliman Elshikh; Bibi Zainab; Zainab Ayaz; Lijun You; Mona S. Alwahibi; Arshad Mehmood Abbasi. 2021. "Comparative assessment of polyphenolics’ content, free radicals’ scavenging and cellular antioxidant potential in apricot fruit." Journal of King Saud University - Science 33, no. 5: 101459.
The issue of foraging for wild food plants among migrants and relocated communities is an important one in environmental studies, especially in order to understand how human societies rearrange their practices linked to nature and how they adapt to new socioecological systems. This paper addresses the complexity of Traditional/Local Environmental Knowledge (LEK) changes associated to wild vegetables and herbs across four different groups of Afghan refugees living in Mansehra District, NW Pakistan, since 1985. Via interviews with eighty study participants, forty-eight wild vegetables and herbs were recorded, representing both the past and present wild plant gastronomic heritage. The majority of the quoted wild plant ingredients were only remembered and no longer actively used, thus suggesting an important erosion of LEK. Moreover, the number of wild vegetables and herbs currently used by Afghan Pashtuns engaged in farming activities is much higher than those reported by the other groups. The findings indicate that practiced LEK, i.e., knowledge that is continuously kept alive via constant contact with the natural environment, is essential for the resilience of the biocultural heritage, which is, however, also influenced by the rearrangement of social life adopted by refugees after relocation.
Ajmal Manduzai; Arshad Abbasi; Shujaul Khan; Abdullah Abdullah; Julia Prakofjewa; Mohammad Amini; Muhammad Amjad; Kevin Cianfaglione; Michele Fontefrancesco; Renata Soukand; Andrea Pieroni. The Importance of Keeping Alive Sustainable Foraging Practices: Wild Vegetables and Herbs Gathered by Afghan Refugees Living in Mansehra District, Pakistan. Sustainability 2021, 13, 1500 .
AMA StyleAjmal Manduzai, Arshad Abbasi, Shujaul Khan, Abdullah Abdullah, Julia Prakofjewa, Mohammad Amini, Muhammad Amjad, Kevin Cianfaglione, Michele Fontefrancesco, Renata Soukand, Andrea Pieroni. The Importance of Keeping Alive Sustainable Foraging Practices: Wild Vegetables and Herbs Gathered by Afghan Refugees Living in Mansehra District, Pakistan. Sustainability. 2021; 13 (3):1500.
Chicago/Turabian StyleAjmal Manduzai; Arshad Abbasi; Shujaul Khan; Abdullah Abdullah; Julia Prakofjewa; Mohammad Amini; Muhammad Amjad; Kevin Cianfaglione; Michele Fontefrancesco; Renata Soukand; Andrea Pieroni. 2021. "The Importance of Keeping Alive Sustainable Foraging Practices: Wild Vegetables and Herbs Gathered by Afghan Refugees Living in Mansehra District, Pakistan." Sustainability 13, no. 3: 1500.
The parameters for pressurized microwave-assisted extraction (PMAE) of total flavonoids (TFs) from Gnaphalium affine D. Don were investigated and the effect of PMAE on its cell wall and tissue structure was analyzed via scanning electron microscopy (SEM). Afterwards, its extraction effect was compared with that of microwave-assisted extraction (MAE), soxhlet extraction (SE) and heat reflux extraction (HRE). After purifying by AB-8 macroporous resin, the in vitro and in vivo antioxidant activities of purified TFs were evaluated by five different chemical assays and alcohol-induced model, respectively. Our findings revealed that an ethanol volume fraction of 81.3%, a L/S ratio of 25.4 mL/g and an extraction time of 138 s, give maximum yield of TFs (1.35 ± 0.02%). PMAE was more efficient and rapid in extracting TFs from G. affine because it cracked the cell wall and disrupted the tissue structure, as observed by SEM. Compared with microwave-assisted extraction (MAE), soxhlet extraction (SE) and heat reflux extraction (HRE), PMAE was more efficient and rapid technique for the extraction of TFs from G. affine. TFs from G. affine showed notable free radical, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt (ABTS) cation, scavenging abilities and reduction ability in vitro. It could also significantly reduce the level of malondialdehyde (MDA), enhance the activity of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC), and increase the level of glutathione (GSH) in the liver of mice with oxidative damage in a dose-dependent manner. Therefore, it showed good antioxidant capacity in vivo. TFs from G. affine indicated good in vitro and in vivo antioxidant activities, which suggests their potential in functional foods and pharmaceuticals as natural antioxidants agents.
Chao Li; Erpei Wang; Mohamed S. Elshikh; Mona S. Alwahibi; Weidong Wang; Gangcheng Wu; Yingbin Shen; Arshad Mehmood Abbasi; Sharui Shan. Extraction and purification of total flavonoids from Gnaphalium affine D. Don and their evaluation for free radicals’ scavenging and oxidative damage inhabitation potential in mice liver. Arabian Journal of Chemistry 2021, 14, 103006 .
AMA StyleChao Li, Erpei Wang, Mohamed S. Elshikh, Mona S. Alwahibi, Weidong Wang, Gangcheng Wu, Yingbin Shen, Arshad Mehmood Abbasi, Sharui Shan. Extraction and purification of total flavonoids from Gnaphalium affine D. Don and their evaluation for free radicals’ scavenging and oxidative damage inhabitation potential in mice liver. Arabian Journal of Chemistry. 2021; 14 (3):103006.
Chicago/Turabian StyleChao Li; Erpei Wang; Mohamed S. Elshikh; Mona S. Alwahibi; Weidong Wang; Gangcheng Wu; Yingbin Shen; Arshad Mehmood Abbasi; Sharui Shan. 2021. "Extraction and purification of total flavonoids from Gnaphalium affine D. Don and their evaluation for free radicals’ scavenging and oxidative damage inhabitation potential in mice liver." Arabian Journal of Chemistry 14, no. 3: 103006.
Zootherapy is accepted all around the globe not only in ancient cultures but different animal derived medicines are also part of the practice in the modern health care systems. The present study assessed the traditional ethnozoological usage of wild animals by local inhabitants in Ladakh region, India, and the reference data for scientific approaches for protection of faunal diversity in trans-Himalayas. The ethnozoological documentation of the animals in Ladakh was carried out through semistructured and close-ended questionnaire surveys and interviews. Multivariate ecological community analysis was used to elucidate the relationship between ethnozoological usage and animal species. Our results showed three animal usage clusters with 32% similarity. Moreover, the similarity in animal usage between digging tools, trophy, handle of tools, decoration, and matting, showed less than 32% of similarity. The highest priority of local people was for food followed by decoration and medicinal usage. The most frequently used animal parts were meat followed by fur and horn. Medicinal uses of 48% of the reported species, i.e., Alectoris chukar (chukar), Cuon alpinus (Asiatic wild dog), Lepus oiostolus (hares), Marmota himalayana (marmots), Ovis aries vignei (Ladakh urial), Pantholops hodgsonii (Tibetan antelope), Procapra picticaudata (Tibetan gazelle), Pseudois nayaur (blue sheep), Tetraogallus himalayensis (Himalayan snow), Tetraogallus tibetanus (Tibetan snow cock), and Lutra lutra (common otter) were reported for the first time from this region. Our study provides innovative information regarding the ethnozoological knowledge in the Ladakh region and reference data for policymakers, researchers, land managers, common public, and the other stakeholders to develop logical and scientific approaches for sustainable use of faunal diversity in hotspot regions like trans-Himalayas and other similar biodiversity-rich sites.
Shiekh Marifatul Haq; Eduardo Soares Calixto; Umer Yaqoob; Riyaz Ahmed; Ahmed Hossam Mahmoud; Rainer W. Bussmann; Osama B. Mohammed; Khalid Ahmad; Arshad Mehmood Abbasi. Traditional Usage of Wild Fauna among the Local Inhabitants of Ladakh, Trans-Himalayan Region. Animals 2020, 10, 2317 .
AMA StyleShiekh Marifatul Haq, Eduardo Soares Calixto, Umer Yaqoob, Riyaz Ahmed, Ahmed Hossam Mahmoud, Rainer W. Bussmann, Osama B. Mohammed, Khalid Ahmad, Arshad Mehmood Abbasi. Traditional Usage of Wild Fauna among the Local Inhabitants of Ladakh, Trans-Himalayan Region. Animals. 2020; 10 (12):2317.
Chicago/Turabian StyleShiekh Marifatul Haq; Eduardo Soares Calixto; Umer Yaqoob; Riyaz Ahmed; Ahmed Hossam Mahmoud; Rainer W. Bussmann; Osama B. Mohammed; Khalid Ahmad; Arshad Mehmood Abbasi. 2020. "Traditional Usage of Wild Fauna among the Local Inhabitants of Ladakh, Trans-Himalayan Region." Animals 10, no. 12: 2317.
Background The present study is the first quantitative ethnobotanical evaluation of Harighal, an inaccessible and unexplored area of District Bagh Azad Jammu & Kashmir (AJK). The exploration, quantification, and comparison of ethnobotanical knowledge among different rural communities of the study area were mainly focused during field survey. Methodology In total, 79 informants (49 men and 34 women) were selected randomly to collect data using a semi-structured questionnaire. Various quantitative indices, including use value, relative frequency of citation, relative importance, fidelity level, and informant consent factor, were employed to evaluate the gathered information. Furthermore, primary data were also compared with twenty-two papers published from adjoining areas. Result A total of 150 medicinal plants belonging to 98 genera and 60 families were documented. Asteraceae, Fabaceae, and Rosaceae were the dominant families having 15 species each. Of these, 76 species were indigenous, 74 exotic, 136 were collected in the wild, 10 cultivated, and 4 both wild-collected and cultivated. Herbaceous taxa were the most used life form, and leaves were the most exploited plant part. Decoctions were the most preferred method used in preparation of herbal recipes. Three species viz. Mentha longifolia, Berberis lycium, and Galium aparine had the highest use value (1.05), relative frequency of citation (0.81), and relative importance value (96), respectively. The highest informant consensus factor (ICF) was reported for digestive disorders. Mentha longifolia, Punica granatum, Zanthoxylum alatum, and Olea ferruginea had 100% fidelity values. The Jaccard index revealed that uses of plants were more similar in two neighboring areas, i.e., Pearl Valley and Toli Peer. Conclusion Local inhabitants still prioritize herbal medicines as an effective way to treat a wide variety of ailments. Elders and health practitioners of the study area are well aware of indigenous knowledge about medicinal plants, but young people are not much interested in herbal practices. Thus, valuable knowledge about the use of plants is on the verge of decline.
Muhammad Shoaib Amjad; Ujala Zahoor; Rainer W. Bussmann; Muhammad Altaf; Syed Mubashar Hussain Gardazi; Arshad Mehmood Abbasi. Ethnobotanical survey of the medicinal flora of Harighal, Azad Jammu & Kashmir, Pakistan. Journal of Ethnobiology and Ethnomedicine 2020, 16, 1 -28.
AMA StyleMuhammad Shoaib Amjad, Ujala Zahoor, Rainer W. Bussmann, Muhammad Altaf, Syed Mubashar Hussain Gardazi, Arshad Mehmood Abbasi. Ethnobotanical survey of the medicinal flora of Harighal, Azad Jammu & Kashmir, Pakistan. Journal of Ethnobiology and Ethnomedicine. 2020; 16 (1):1-28.
Chicago/Turabian StyleMuhammad Shoaib Amjad; Ujala Zahoor; Rainer W. Bussmann; Muhammad Altaf; Syed Mubashar Hussain Gardazi; Arshad Mehmood Abbasi. 2020. "Ethnobotanical survey of the medicinal flora of Harighal, Azad Jammu & Kashmir, Pakistan." Journal of Ethnobiology and Ethnomedicine 16, no. 1: 1-28.
Modification of pathogenic strains with the passage of time are responsible for evolution in the timeline of vaccine development for last 30 years. Recent advancements in computational vaccinology on one hand and genome sequencing approaches on the other have generated new hopes in vaccine development. The aim of this review was to discuss the evolution of vaccines, their characteristics as well as limitations. In this review, we highlighted the evolution of vaccines, from first generation to the current status, pointing out how different vaccines have emerged and different approaches that are being followed up in the development of more rational vaccines against a wide range of diseases. Data were collected using Google Scholar, Web of Science, Science Direct, Web of Knowledge, Scopus, and Science hub. Whereas, computational tools like (NCBI, GeneMANIA and STRING) were used to analyze the pathways of vaccines action. Innovative tools, such as computational tools, recombinant technologies and intra‐dermal devices, are currently being investigated in order to improve the immunological response. New technologies enlightened the interactions of host proteins with pathogenic proteins for vaccine candidate development but still there is a need of integrating transcriptomic and proteomic approaches. Although immunization with genomics data is a successful approach, its advantages must be assessed case by case and its applicability depends on the nature of the agent to be immunized, the nature of the antigen and the type of immune response required to achieve effective protection.
Guangli Lu; Sharui Shan; Bibi Zainab; Zainab Ayaz; Jialiang He; Zhenxing Xie; Umer Rashid; Dalin Zhang; Arshad Mehmood Abbasi. Novel vaccine design based on genomics data analysis: A review. Scandinavian Journal of Immunology 2020, 93, e12986 .
AMA StyleGuangli Lu, Sharui Shan, Bibi Zainab, Zainab Ayaz, Jialiang He, Zhenxing Xie, Umer Rashid, Dalin Zhang, Arshad Mehmood Abbasi. Novel vaccine design based on genomics data analysis: A review. Scandinavian Journal of Immunology. 2020; 93 (3):e12986.
Chicago/Turabian StyleGuangli Lu; Sharui Shan; Bibi Zainab; Zainab Ayaz; Jialiang He; Zhenxing Xie; Umer Rashid; Dalin Zhang; Arshad Mehmood Abbasi. 2020. "Novel vaccine design based on genomics data analysis: A review." Scandinavian Journal of Immunology 93, no. 3: e12986.
Background: The present study is the first quantitative ethnobotanical evaluation of Harighal, an inaccessible and unexplored area of District Bagh Azad Jammu and Kashmir (AJK). The exploration, quantification and comparison of ethnobotanical knowledge among different rural communities of the study area were mainly focused during field survey.Methodology: In total, 79 informants (49 men and 34 women) were selected randomly to collect data using a semi-structured questionnaire. Various quantitative indices, including use value, relative frequency of citation, relative importance, fidelity level and informant consent factor were employed to evaluate the gathered information. Furthermore, primary data were also compared with twenty-two papers published from adjoining areas.Result: A total of 150 medicinal plants belonging to 98 genera and 60 families were documented. Asteraceae, Fabaceae and Rosaceae were the dominant families having 15 species each. Of these 76 species were indigenous, 74 exotic, 136 were collected in the wild, 10 cultivated and 4 both wild-collected and cultivated. Herbaceous taxa were the most used life form, and leaves were the most exploited plant part. Decoctions were the most preferred method used in preparation of herbal recipes. Three species viz. Mentha longifolia, Berberis lycium and Galium aparine had the highest use value (1.05), relative frequency of citation (0.81) and relative importance value (96), respectively. The highest informant consensus factor (ICF) was reported for digestive disorders. Mentha longifolia, Punica granatum, Zanthoxylum alatum and Olea ferruginea had 100% fidelity values. The Jaccard index revealed that uses of plants were more similar in two neighboring areas i.e. Pearl Valley and Toli Peer.Conclusion: Local inhabitants still prioritize herbal medicines as an effective way to treat a wide variety of ailments. Elders and health practitioners of the study area are well aware of indigenous knowledge about medicinal plants, but young people are not much interested in herbal practices. Thus, valuable knowledge about the use of plants is on the verge of decline.
Muhammad Shoaib Amjad; Ujala Zahoor; Rainer W. Bussmann; Muhammad Altaf; Syed Mubashar Hussain Gardazi; Arshad Mehmood Abbasi. Ethnobotanical survey of the medicinal flora of Harighal, Azad Jammu & Kashmir, Pakistan. 2020, 1 .
AMA StyleMuhammad Shoaib Amjad, Ujala Zahoor, Rainer W. Bussmann, Muhammad Altaf, Syed Mubashar Hussain Gardazi, Arshad Mehmood Abbasi. Ethnobotanical survey of the medicinal flora of Harighal, Azad Jammu & Kashmir, Pakistan. . 2020; ():1.
Chicago/Turabian StyleMuhammad Shoaib Amjad; Ujala Zahoor; Rainer W. Bussmann; Muhammad Altaf; Syed Mubashar Hussain Gardazi; Arshad Mehmood Abbasi. 2020. "Ethnobotanical survey of the medicinal flora of Harighal, Azad Jammu & Kashmir, Pakistan." , no. : 1.
Gadani shipbreaking area, located on the coastline of Pakistan, is an important fish production area. In this study, levels of four metals (Ni, Pb, Cd, and Mn) in 148 muscle and gill samples of seven fish species (Small-scale terapon, Torpedo scade, Sicklefish, Saddle grunt, Gold silk seabream, Indian mackerel, Spotted sickle fish) and seawater samples, taken from 9 sampling sites in the shipbreaking area, were determined. In addition, multiple approaches were used to assess human health risks from fish consumption. Trace metal concentration in seawater ranged from 0.05 to 1.96 mg/L in shipbreaking vicinity and 0.03 to 0.97 mg/L in the reference site (Miani Hor). However, metal accumulations in fish species ranged from 1.33 to 5.26 μg/g. Among trace metals, the level of Pb in all fish species was highest, followed by Ni, Mn, and Cd. The bioaccumulation factors (BAFs) for both gills and muscles displayed the order: Mn > Cd > Ni > Pb. Estimated daily intake (EDI) values were below the tolerable daily intake (TDI). Based on target hazard quotient (THQ), the investigated fish species were safe regarding Pb and Mn (THQ < 1), while they may cause potential risk regarding Cd and Ni (THQ > 1). After comparison with maximum permissible limits, heavy metal concentration in the edible muscle tissues of all the analyzed fish species from the Gadani coast were found safe for human consumption.
Allauddin Kakar; Malik Hayat; Arshad Abbasi; Arshid Pervez; Qaisar Mahmood; Umar Farooq; Tahir Akbar; Shafaqat Ali; Muhammad Rizwan; Hamed El-Serehy; Mohamed Abdel-Daim. Risk Assessment of Heavy Metals in Selected Marine Fish Species of Gadani Shipbreaking Area and Pakistan. Animals 2020, 10, 1738 .
AMA StyleAllauddin Kakar, Malik Hayat, Arshad Abbasi, Arshid Pervez, Qaisar Mahmood, Umar Farooq, Tahir Akbar, Shafaqat Ali, Muhammad Rizwan, Hamed El-Serehy, Mohamed Abdel-Daim. Risk Assessment of Heavy Metals in Selected Marine Fish Species of Gadani Shipbreaking Area and Pakistan. Animals. 2020; 10 (10):1738.
Chicago/Turabian StyleAllauddin Kakar; Malik Hayat; Arshad Abbasi; Arshid Pervez; Qaisar Mahmood; Umar Farooq; Tahir Akbar; Shafaqat Ali; Muhammad Rizwan; Hamed El-Serehy; Mohamed Abdel-Daim. 2020. "Risk Assessment of Heavy Metals in Selected Marine Fish Species of Gadani Shipbreaking Area and Pakistan." Animals 10, no. 10: 1738.
Biofilm forming ability of Pseudomonas aeruginosa make them vulnerable, because it makes them recalcitrant against various antibiotics. Quorum sensing (QS) is cell density based signaling that helps in bacterial cell–cell communication, which regulated various virulence factors such as pigment and biofilm formation that contribute in the establishment of chronic infections. The interruption of QS is one of the effective approach to control various virulence factors. Present study was intended with the aim to authenticate antibiofilm potential in different solvents based extracts of selected medicinal plant species viz. Berginia ciliata, Clematis grata and Clematis viticella traditionally used by the inhabitants of Himalayan region of Pakistan to treat various pathogenic diseases. P. aeruginosa PAO1, an opportunistic pathogen and involves in various life-threatening infections specifically in immune deficient patients was used as a model pathogen. Plants were extracted in various organic (ethanol, methanol, acetone, ethyl acetate, hexane, chloroform) as well as in aqueous solvents and their ability to inhibit biofilm was measured. Biofilm of PAO1 was grown in Jensen's medium while growing at 30 °C and crystal violet assay was performed to assess the biofilm inhibiting activity of plant extracts. Solvents play a vital role in extraction of plant components and it was found that the plants in various solvents exhibit different activity against the PAO1 biofilm. Comparatively, 1% methanolic extract of B. ciliata (rhizome with skin), showed more than 80% inhibition of biofilm formation without effecting on the growth of the bacterium. Significant correlation between flavonoids content and antibiofilm activity in methanolic extract revealed the contribution of secondary metabolites in P. aeruginosa (PAO1) biofilm inhibition. Our study revealed that plants under investigation more specifically B. ciliata could be a potential candidate for drug discovery to treat P. aeruginosa PAO1, induced infectious diseases especially for its biofilm treatment.
Khan Alam; Dunia A. Al Farraj; Syeda Mah-E-Fatima; Muhammad Arfat Yameen; Mohamed Soliman Elshikh; Roua M. Alkufeidy; Abd El-Zaher M.A. Mustafa; Pramod Bhasme; Maryam K. Alshammari; Noorah A. Alkubaisi; Arshad Mehmood Abbasi; Tatheer Alam Naqvi. Anti-biofilm activity of plant derived extracts against infectious pathogen-Pseudomonas aeruginosa PAO1. Journal of Infection and Public Health 2020, 13, 1734 -1741.
AMA StyleKhan Alam, Dunia A. Al Farraj, Syeda Mah-E-Fatima, Muhammad Arfat Yameen, Mohamed Soliman Elshikh, Roua M. Alkufeidy, Abd El-Zaher M.A. Mustafa, Pramod Bhasme, Maryam K. Alshammari, Noorah A. Alkubaisi, Arshad Mehmood Abbasi, Tatheer Alam Naqvi. Anti-biofilm activity of plant derived extracts against infectious pathogen-Pseudomonas aeruginosa PAO1. Journal of Infection and Public Health. 2020; 13 (11):1734-1741.
Chicago/Turabian StyleKhan Alam; Dunia A. Al Farraj; Syeda Mah-E-Fatima; Muhammad Arfat Yameen; Mohamed Soliman Elshikh; Roua M. Alkufeidy; Abd El-Zaher M.A. Mustafa; Pramod Bhasme; Maryam K. Alshammari; Noorah A. Alkubaisi; Arshad Mehmood Abbasi; Tatheer Alam Naqvi. 2020. "Anti-biofilm activity of plant derived extracts against infectious pathogen-Pseudomonas aeruginosa PAO1." Journal of Infection and Public Health 13, no. 11: 1734-1741.
The present study was intendant to evaluate variations among flaxseed varities in terms of fatty acid composition, phytochemical profiles, and antioxidant activities determined by oxygen radical absorbance capacity (ORAC), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferrous ion reducing antioxidant power (FRAP) assays. Significant variations in the fatty acid composition, phenolic acids and lignan were observed in flaxseed varieties from different countries. Among these flaxseed verities, the unsaturated fatty acids accounted over four fifths of total fatty acid contents. The highest ratio of linolenic acid of total fatty acid was observed in USPEA, whereas the lowest one was found in Yexiao. USPEA showed the most contents of total phenolics, as well as flaxseed lignans. In general, total phenolics appeared to be the main contributors in the antioxidant capacity of flaxseed, which presented significant positive correlation. Our study revealed that both cultivar and origin of seeds significantly affect fatty acid composition, phenolic acids, lignans and subsequent antioxidant activities in flaxseed. The results provide new aspects of breeding resources of flaxseed cultivars by presenting their quality specification and possible commercial value.
Caisheng Qiu; Hong Wang; Yuan Guo; Songhua Long; Yufu Wang; Arshad Mehmood Abbasi; Xinbo Guo; Devra I. Jarvis. Comparison of fatty acid composition, phytochemical profile and antioxidant activity in four flax (Linum usitatissimum L.) varieties. Oil Crop Science 2020, 5, 136 -141.
AMA StyleCaisheng Qiu, Hong Wang, Yuan Guo, Songhua Long, Yufu Wang, Arshad Mehmood Abbasi, Xinbo Guo, Devra I. Jarvis. Comparison of fatty acid composition, phytochemical profile and antioxidant activity in four flax (Linum usitatissimum L.) varieties. Oil Crop Science. 2020; 5 (3):136-141.
Chicago/Turabian StyleCaisheng Qiu; Hong Wang; Yuan Guo; Songhua Long; Yufu Wang; Arshad Mehmood Abbasi; Xinbo Guo; Devra I. Jarvis. 2020. "Comparison of fatty acid composition, phytochemical profile and antioxidant activity in four flax (Linum usitatissimum L.) varieties." Oil Crop Science 5, no. 3: 136-141.
Background Like botanical taxa, various species of animals are also used in traditional and modern health care systems. Present study was intended with the aim to document the traditional uses of herptile and fish species among the local communities in the vicinity of the River Chenab, Punjab Pakistan. Method Data collected by semi-structured interviews and questionnaires were subsequently analyzed using relative frequency of citation (FC), fidelity level (FL), relative popularity level (RPL), similarity index (SI), and rank order priority (ROP) indices. Results Out of total 81 reported species, ethnomedicinal uses of eight herptiles viz. Aspideretes gangeticus, A. hurum, Eublepharis macularius, Varanus bengalensis, Python molurus, Eryx johnii, Ptyas mucosus mucosus, Daboia russelii russelii and five fish species including Hypophthalmichthys molitrix, Cirrhinus reba, Labeo dero, Mastacembelus armatus, and Pethia ticto were reported for the first time from this region. Fat, flesh, brain, and skin were among the commonly utilized body parts to treat allergy, cardiovascular, nervous and respiratory disorders, sexual impotency, skin infections, and as antidote and anti-diabetic agents. Hoplobatrachus tigerinus, Duttaphrynus stomaticus, and Ptyas mucosus mucosus (herptiles), as well as Labeo rohita, Wallago attu, and Cirrhinus reba (fish) were top ranked with maximum informant reports, frequency of citations, and rank order priority. Uromastyx hardwickii, Ctenopharyngodon idella, H. molitrix, Cirrhinus mrigala, C. reba, L. rohita, L. calbasu, L. dero, and Pethia ticto were the species with 100% fidelity level. Furthermore, medicinal uses of Aspideretes gangeticus, Aspideretes hurum, Calotes versicolor, Daboia russelii russelii, Hypophthalmichthys molitrix, Cirrhinus reba, Labeo dero, Mastacembelus armatus, Pethia ticto, and Gagata cenia were reported for the first time. Conclusion About half of the reported species depicted zero similarity index with previously reported literature, which indicates strong associations of local inhabitants with animal species, particularly for therapeutic purpose. Inclusive studies on composition and bioactivities of the species with maximum use reports may contribute significantly in animal-based novel drugs discovery.
Muhammad Altaf; Arshad Mehmood Abbasi; Muhammad Umair; Muhammad Shoaib Amjad; Kinza Irshad; Abdul Majid Khan. The use of fish and herptiles in traditional folk therapies in three districts of Chenab riverine area in Punjab, Pakistan. Journal of Ethnobiology and Ethnomedicine 2020, 16, 1 -21.
AMA StyleMuhammad Altaf, Arshad Mehmood Abbasi, Muhammad Umair, Muhammad Shoaib Amjad, Kinza Irshad, Abdul Majid Khan. The use of fish and herptiles in traditional folk therapies in three districts of Chenab riverine area in Punjab, Pakistan. Journal of Ethnobiology and Ethnomedicine. 2020; 16 (1):1-21.
Chicago/Turabian StyleMuhammad Altaf; Arshad Mehmood Abbasi; Muhammad Umair; Muhammad Shoaib Amjad; Kinza Irshad; Abdul Majid Khan. 2020. "The use of fish and herptiles in traditional folk therapies in three districts of Chenab riverine area in Punjab, Pakistan." Journal of Ethnobiology and Ethnomedicine 16, no. 1: 1-21.
A geographically diverse group of 29 ethnobiologists addresses three common themes in response to the COVID-19 global health crisis: impact on local communities, future interactions between researchers and communities, and new (or renewed) conceptual and/or applied research priorities for ethnobiology.
Ina Vandebroek; Andrea Pieroni; John Richard Stepp; Natalia Hanazaki; Ana Ladio; Rômulo Romeu Nóbrega Alves; David Picking; Rupika Delgoda; Alfred Maroyi; Tinde Van Andel; Cassandra L. Quave; Narel Y. Paniagua-Zambrana; Rainer W Bussmann; Guillaume Odonne; Arshad Mehmood Abbasi; Ulysses Paulino Albuquerque; Janelle Baker; Susan Kutz; Shrabya Timsina; Masayoshi Shigeta; Tacyana Pereira Ribeiro Oliveira; Julio A. Hurrell; Patricia M. Arenas; Jeremias P. Puentes; Jean Hugé; Yeter Yeşil; Laurent Jean Pierre; Temesgen Magule Olango; Farid Dahdouh-Guebas. Reshaping the future of ethnobiology research after the COVID-19 pandemic. Nature Plants 2020, 6, 723 -730.
AMA StyleIna Vandebroek, Andrea Pieroni, John Richard Stepp, Natalia Hanazaki, Ana Ladio, Rômulo Romeu Nóbrega Alves, David Picking, Rupika Delgoda, Alfred Maroyi, Tinde Van Andel, Cassandra L. Quave, Narel Y. Paniagua-Zambrana, Rainer W Bussmann, Guillaume Odonne, Arshad Mehmood Abbasi, Ulysses Paulino Albuquerque, Janelle Baker, Susan Kutz, Shrabya Timsina, Masayoshi Shigeta, Tacyana Pereira Ribeiro Oliveira, Julio A. Hurrell, Patricia M. Arenas, Jeremias P. Puentes, Jean Hugé, Yeter Yeşil, Laurent Jean Pierre, Temesgen Magule Olango, Farid Dahdouh-Guebas. Reshaping the future of ethnobiology research after the COVID-19 pandemic. Nature Plants. 2020; 6 (7):723-730.
Chicago/Turabian StyleIna Vandebroek; Andrea Pieroni; John Richard Stepp; Natalia Hanazaki; Ana Ladio; Rômulo Romeu Nóbrega Alves; David Picking; Rupika Delgoda; Alfred Maroyi; Tinde Van Andel; Cassandra L. Quave; Narel Y. Paniagua-Zambrana; Rainer W Bussmann; Guillaume Odonne; Arshad Mehmood Abbasi; Ulysses Paulino Albuquerque; Janelle Baker; Susan Kutz; Shrabya Timsina; Masayoshi Shigeta; Tacyana Pereira Ribeiro Oliveira; Julio A. Hurrell; Patricia M. Arenas; Jeremias P. Puentes; Jean Hugé; Yeter Yeşil; Laurent Jean Pierre; Temesgen Magule Olango; Farid Dahdouh-Guebas. 2020. "Reshaping the future of ethnobiology research after the COVID-19 pandemic." Nature Plants 6, no. 7: 723-730.
A wild food ethnobotanical field study was conducted in the Ishkoman and Yasin valleys, located in the Hindukush Mountain Range of Gilgit-Baltistan, northern Pakistan. These valleys are inhabited by diverse, often marginalized, linguistic and religious groups. The field survey was conducted via one hundred and eighty semistructured interviews to record data in nine villages. Forty gathered wild food botanical and mycological taxa were recorded and identified. Comparative analysis among the different linguistic and religious groups revealed that the gathered wild food plants were homogenously used. This may be attributed to the sociocultural context of the study area, where most of the population professes the Ismaili Shia Islamic faith, and to the historical stratifications of different populations along the centuries, which may have determined complex adaptation processes and exchange of possibly distinct pre-existing food customs. A few wild plants had very rarely or never been previously reported as food resources in Pakistan, including Artemisia annua, Hedysarum falconeri, Iris hookeriana, Lepidium didymium and Saussurea lappa. Additionally, the recorded local knowledge is under threat and we analyzed possible factors that have caused this change. The recorded biocultural heritage could, however, represent a crucial driver, if properly revitalized, for assuring the food security of the local communities and also for further developing ecotourism and associated sustainable gastronomic initiatives in the area.
Muhammad Abdul Aziz; Arshad Mehmood Abbasi; Zahid Ullah; Andrea Pieroni. Shared but Threatened: The Heritage of Wild Food Plant Gathering among Different Linguistic and Religious Groups in the Ishkoman and Yasin Valleys, North Pakistan. Foods 2020, 9, 601 .
AMA StyleMuhammad Abdul Aziz, Arshad Mehmood Abbasi, Zahid Ullah, Andrea Pieroni. Shared but Threatened: The Heritage of Wild Food Plant Gathering among Different Linguistic and Religious Groups in the Ishkoman and Yasin Valleys, North Pakistan. Foods. 2020; 9 (5):601.
Chicago/Turabian StyleMuhammad Abdul Aziz; Arshad Mehmood Abbasi; Zahid Ullah; Andrea Pieroni. 2020. "Shared but Threatened: The Heritage of Wild Food Plant Gathering among Different Linguistic and Religious Groups in the Ishkoman and Yasin Valleys, North Pakistan." Foods 9, no. 5: 601.
Moringa oleifera is also known as “Miracle tree”, due to its multiple uses and adaptability. Because of nutritive and pharmacological values, it is widely cultivated across the world. M. oleifera leaves are rich source of minerals, vitamins and many health beneficial secondary metabolites, and possess significant anti-diabetic potential. Consequently, Insilco study could be noteworthy to expand effective anti-diabetic drugs from this plant. Present study was designed to find out the best bioactive compounds of M. oleifera as a potential therapeutic agent against diabetes mellitus through In-silico method. For this, structures of phytochemicals were extracted from PubChem and docked to mutated protein from PBD. Afterwards, datasets were prepared for ligand based pharmacophore and their pharmacophoric features were generated from LigandScout. Finally five phytochemicals viz. anthraquinone, 2-phenylchromenylium (Anthocyanins), hemlock tannin, sitogluside (glycoside) and A-phenolic steroid were selected, which exhibited effective binding within the active binding pocket of the targeted protein. Ligand based pharmacophore model showed the key features i.e. HBD, HBA, aromatic ring, hydrophobic, positively ionizable surface essential for receptor binding. Our findings suggest that screened phytochemicals present in M. oleifera can be used as potential therapeutic drug candidates to treat diabetes mellitus.
Bibi Zainab; Zainab Ayaz; Mona S. Alwahibi; Sajid Khan; Humaira Rizwana; Dina Wafik Soliman; Asma Alawaad; Arshad Mehmood Abbasi. In-silico elucidation of Moringa oleifera phytochemicals against diabetes mellitus. Saudi Journal of Biological Sciences 2020, 27, 2299 -2307.
AMA StyleBibi Zainab, Zainab Ayaz, Mona S. Alwahibi, Sajid Khan, Humaira Rizwana, Dina Wafik Soliman, Asma Alawaad, Arshad Mehmood Abbasi. In-silico elucidation of Moringa oleifera phytochemicals against diabetes mellitus. Saudi Journal of Biological Sciences. 2020; 27 (9):2299-2307.
Chicago/Turabian StyleBibi Zainab; Zainab Ayaz; Mona S. Alwahibi; Sajid Khan; Humaira Rizwana; Dina Wafik Soliman; Asma Alawaad; Arshad Mehmood Abbasi. 2020. "In-silico elucidation of Moringa oleifera phytochemicals against diabetes mellitus." Saudi Journal of Biological Sciences 27, no. 9: 2299-2307.
Novel coronavirus (2019-nCoV), since its emergence from Wuhan China in December 31, 2019 is still uncontrolled and has raised attention around the globe. According to World health organization, up to March 20, 2020, globally 209,839 confirmed cases of COVID-19 have been reported along with 8778 deaths. 2019-nCoV is likely to be a recombinant of different coronaviruses such as SARS CoV and MERS CoV. Recent developments revealed that glycosylated spike (S) protein of 2019-nCov is contributing significantly in facilitating 2019- nCov infection in human body. The subunit (S1) of spike protein facilitates 2019-nCov binding with host cells’ receptors, while S2 subunit (post fusion core of 2019-nCov) is a key factor in fusion of 2019-nCov with host cell membrane and subsequent inoculation of its DNA in to the host cell. Therefore, in coronavirus infection, membrane fusion and receptor binding are critical. And if active sites of 2019-nCov spike protein S2 (post fusion core of 2019-nCov) are blocked, this may reduce COVID-19 infections in human. We use clustering based drug-drug interaction (DDI) networks and drug repositioning approach based on modularity to inhibit the membrane fusion and receptor binding capacity of 2019-nCov. About 150 drug compounds effective against SARS-CoV and MERS-CoV were retrieved, and screened on the basis of Lipinski rule of five. Clusters and strongly interacted DDI networks were generated in accordance to their modularity class, average path length and density. Promising drug candidates were then filtered by toxicity indicator and molecular docking. Our finding reveals that ZINC000029038525 and ZINC000029129064 drug compounds have significant binding potential with active sites of post fusion core of 2019-nCov ‘S2’ subunit and may inhibit membrane fusion and receptor binding capacity of 2019-nCov. Therefore, these drug compounds alone or in amalgamation could be strong and more effective therapeutic candidates against 2019-nCov infections.
Zainab Ayaz; Bibi Zainab; Arshad Mehmood Abbasi. In silico elucidation revealed SARS CoV and MERS CoV Drug Compounds could be Potential Therapeutic Candidates against Post Fusion Core (S2) Protein of Novel Coronavirus (2019-nCov). 2020, 1 .
AMA StyleZainab Ayaz, Bibi Zainab, Arshad Mehmood Abbasi. In silico elucidation revealed SARS CoV and MERS CoV Drug Compounds could be Potential Therapeutic Candidates against Post Fusion Core (S2) Protein of Novel Coronavirus (2019-nCov). . 2020; ():1.
Chicago/Turabian StyleZainab Ayaz; Bibi Zainab; Arshad Mehmood Abbasi. 2020. "In silico elucidation revealed SARS CoV and MERS CoV Drug Compounds could be Potential Therapeutic Candidates against Post Fusion Core (S2) Protein of Novel Coronavirus (2019-nCov)." , no. : 1.
Riffat Parveen; Arshad M. Abbasi; Nazia Shaheen; Munir H. Shah. Accumulation of selected metals in the fruits of medicinal plants grown in urban environment of Islamabad, Pakistan. Arabian Journal of Chemistry 2020, 13, 308 -317.
AMA StyleRiffat Parveen, Arshad M. Abbasi, Nazia Shaheen, Munir H. Shah. Accumulation of selected metals in the fruits of medicinal plants grown in urban environment of Islamabad, Pakistan. Arabian Journal of Chemistry. 2020; 13 (1):308-317.
Chicago/Turabian StyleRiffat Parveen; Arshad M. Abbasi; Nazia Shaheen; Munir H. Shah. 2020. "Accumulation of selected metals in the fruits of medicinal plants grown in urban environment of Islamabad, Pakistan." Arabian Journal of Chemistry 13, no. 1: 308-317.