This page has only limited features, please log in for full access.
To simultaneously detect two metabolites of Aspergillus flavus, namely, cyclopiazonic acid (CPA) and aflatoxin (AFT), an ultrasensitive monoclonal antibody (mAb) YTT-2 against CPA was developed and characterized, with sensitivity to CPA of 1.32 ng mL−1. Along with the previously homemade mAb 1C11 against AFT, two mAbs were used to develop time-resolved fluorescence immunoprobes or gold immunoprobes. We developed two multiple-analyte paper immunosensors including time-resolved fluorescent immunochromatographic assay (TRFICA) and gold immunochromatographic assay (GICA) for the simultaneous determination of CPA and AFT. The TRFICA showed limits of determination (LODs) of 0.21 and 0.004 ng mL−1, while the GICA showed LODs of 0.33 and 0.01 ng mL−1 for CPA and AFT, respectively. To validate the specificity of the two rapid immunoassays, rice, corn and peanut samples were spiked with different concentrations of CPA and AFT. The two methods showed satisfactory recoveries (76.39~90.82% for CPA and 84.60~94.45% for AFT) and coefficients of variation of 3.50~7.80% for CPA and 4.12~13.90% for AFT. The results indicated that the TRFICA could complete the test within 5 min and had lower LODs and linear ranges, compared with that of GICA. The method developed in this work can be widely applied to the rapid and quantitative simultaneous determination of multiple harmful metabolites in fungi for food safety and health care. Graphical abstract
Tingting Yan; Zhaowei Zhang; Qi Zhang; Xiaoqian Tang; Du Wang; Xiaofeng Hu; Wen Zhang; Xiaomei Chen; Peiwu Li. Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies. Microchimica Acta 2020, 187, 1 -8.
AMA StyleTingting Yan, Zhaowei Zhang, Qi Zhang, Xiaoqian Tang, Du Wang, Xiaofeng Hu, Wen Zhang, Xiaomei Chen, Peiwu Li. Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies. Microchimica Acta. 2020; 187 (12):1-8.
Chicago/Turabian StyleTingting Yan; Zhaowei Zhang; Qi Zhang; Xiaoqian Tang; Du Wang; Xiaofeng Hu; Wen Zhang; Xiaomei Chen; Peiwu Li. 2020. "Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies." Microchimica Acta 187, no. 12: 1-8.
Deoxynivalenol (DON) is a secondary metabolite produced by Fusarium, which is a trichothecene mycotoxin. As the main mycotoxin with high toxicity, wheat, barley, corn and their products are susceptible to contamination of DON. Due to the stability of this mycotoxin, traditional methods for DON reduction often require a strong oxidant, high temperature and high pressure with more energy consumption. Therefore, exploring green, efficient and environmentally friendly ways to degrade or reduce DON is a meaningful and challenging issue. Herein, a dendritic-like α-Fe2O3 was successfully prepared using a facile hydrothermal synthesis method at 160 °C, which was systematically characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). It was found that dendritic-like α-Fe2O3 showed superior activity for the photocatalytic degradation of DON in aqueous solution under visible light irradiation (λ > 420 nm) and 90.3% DON (initial concentration of 4.0 μg/mL) could be reduced in 2 h. Most of all, the main possible intermediate products were proposed through high performance liquid chromatography-mass spectrometry (HPLC-MS) after the photocatalytic treatment. This work not only provides a green and promising way to mitigate mycotoxin contamination but also may present useful information for future studies.
Huiting Wang; Jin Mao; Zhaowei Zhang; Qi Zhang; Liangxiao Zhang; Peiwu Li; Wen Zhang. Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation. Toxins 2019, 11, 105 .
AMA StyleHuiting Wang, Jin Mao, Zhaowei Zhang, Qi Zhang, Liangxiao Zhang, Peiwu Li, Wen Zhang. Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation. Toxins. 2019; 11 (2):105.
Chicago/Turabian StyleHuiting Wang; Jin Mao; Zhaowei Zhang; Qi Zhang; Liangxiao Zhang; Peiwu Li; Wen Zhang. 2019. "Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation." Toxins 11, no. 2: 105.
MycoKey, an EU-funded Horizon 2020 project, includes a series of “Roundtable Discussions” to gather information on trending research areas in the field of mycotoxicology. This paper includes summaries of the Roundtable Discussions on Chemical Detection and Monitoring of mycotoxins and on the role of genetics and biodiversity in mycotoxin production. Discussions were managed by using the nominal group discussion technique, which generates numerous ideas and provides a ranking for those identified as the most important. Four questions were posed for each research area, as well as two questions that were common to both discussions. Test kits, usually antibody based, were one major focus of the discussions at the Chemical Detection and Monitoring roundtable because of their many favorable features, e.g., cost, speed and ease of use. The second area of focus for this roundtable was multi-mycotoxin detection protocols and the challenges still to be met to enable these protocols to become methods of choice for regulated mycotoxins. For the genetic and biodiversity group, both the depth and the breadth of trending research areas were notable. For some areas, e.g., microbiome studies, the suggested research questions were primarily of a descriptive nature. In other areas, multiple experimental approaches, e.g., transcriptomics, proteomics, RNAi and gene deletions, are needed to understand the regulation of toxin production and mechanisms underlying successful biological controls. Answers to the research questions will provide starting points for developing acceptable prevention and remediation processes. Forging a partnership between scientists and appropriately-placed communications experts was recognized by both groups as an essential step to communicating risks, while retaining overall confidence in the safety of the food supply and the integrity of the food production chain.
John F. Leslie; Veronica Lattanzio; Kris Audenaert; Paola Battilani; Jeffrey Cary; Sofía N. Chulze; Sarah De Saeger; Annamaria Gerardino; Petr Karlovsky; Yu-Cai Liao; Chris M. Maragos; Giuseppe Meca; Angel Medina; Antonio Moretti; Gary Munkvold; Giuseppina Mulè; Patrick Njobeh; Ivan Pecorelli; Giancarlo Perrone; Amedeo Pietri; Juan M. Palazzini; Robert H. Proctor; Endang S. Rahayu; Maria L. Ramírez; Robert Samson; Jörg Stroka; Michael Sulyok; Mark Sumarah; Cees Waalwijk; Qi Zhang; Hao Zhang; Antonio F. Logrieco. MycoKey Round Table Discussions of Future Directions in Research on Chemical Detection Methods, Genetics and Biodiversity of Mycotoxins. Toxins 2018, 10, 109 .
AMA StyleJohn F. Leslie, Veronica Lattanzio, Kris Audenaert, Paola Battilani, Jeffrey Cary, Sofía N. Chulze, Sarah De Saeger, Annamaria Gerardino, Petr Karlovsky, Yu-Cai Liao, Chris M. Maragos, Giuseppe Meca, Angel Medina, Antonio Moretti, Gary Munkvold, Giuseppina Mulè, Patrick Njobeh, Ivan Pecorelli, Giancarlo Perrone, Amedeo Pietri, Juan M. Palazzini, Robert H. Proctor, Endang S. Rahayu, Maria L. Ramírez, Robert Samson, Jörg Stroka, Michael Sulyok, Mark Sumarah, Cees Waalwijk, Qi Zhang, Hao Zhang, Antonio F. Logrieco. MycoKey Round Table Discussions of Future Directions in Research on Chemical Detection Methods, Genetics and Biodiversity of Mycotoxins. Toxins. 2018; 10 (3):109.
Chicago/Turabian StyleJohn F. Leslie; Veronica Lattanzio; Kris Audenaert; Paola Battilani; Jeffrey Cary; Sofía N. Chulze; Sarah De Saeger; Annamaria Gerardino; Petr Karlovsky; Yu-Cai Liao; Chris M. Maragos; Giuseppe Meca; Angel Medina; Antonio Moretti; Gary Munkvold; Giuseppina Mulè; Patrick Njobeh; Ivan Pecorelli; Giancarlo Perrone; Amedeo Pietri; Juan M. Palazzini; Robert H. Proctor; Endang S. Rahayu; Maria L. Ramírez; Robert Samson; Jörg Stroka; Michael Sulyok; Mark Sumarah; Cees Waalwijk; Qi Zhang; Hao Zhang; Antonio F. Logrieco. 2018. "MycoKey Round Table Discussions of Future Directions in Research on Chemical Detection Methods, Genetics and Biodiversity of Mycotoxins." Toxins 10, no. 3: 109.
Multiple-mycotoxin contamination has been frequently found in the agro-food monitoring due to the coexistence of fungi. However, many determination methods focused on a single mycotoxin, highlighting the demand for on-site determination of multiple mycotoxins in a single run. We develop a multicolor-based immunochromatographic strip (ICS) for simultaneous determination of aflatoxin B1 (AFB1), zearalenone (ZEN) and T-2 toxin in maize- and cereal-based animal feeds. The nanoparticles with different colors are conjugated with three monoclonal antibodies, which serve as the immunoassay probes. The decrease in color intensity is observed by the naked eyes, providing simultaneous quantification of three mycotoxins. The visible limits of detection for AFB1, ZEN and T-2 are estimated to be 0.5, 2, and 30 ng/mL, respectively. The cut-off values are 1, 10, and 50 ng/mL, respectively. Considerable specificity and stability are found using real samples. The results are in excellent agreement with those from high-performance liquid chromatography/tandem mass spectrometry. The multi-color ICS boasts sensitive and rapid visual differentiation and simultaneous semi-quantification of aflatoxin B1, zearalenone and T-2 toxin in maize- and cereal-based feed samples within 20 min.
Lin Xu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Du Wang; Hui Li; Peiwu Li. An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay. Toxins 2018, 10, 87 .
AMA StyleLin Xu, Zhaowei Zhang, Qi Zhang, Wen Zhang, Li Yu, Du Wang, Hui Li, Peiwu Li. An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay. Toxins. 2018; 10 (2):87.
Chicago/Turabian StyleLin Xu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Du Wang; Hui Li; Peiwu Li. 2018. "An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay." Toxins 10, no. 2: 87.
Delamination of layer semiconductor materials into two-dimensional sheets can bring superior properties such as surface area, intrinsic carrier mobility and band structure. In this study, inerratic nanosized graphitic carbon nitride (g-C3N4) sheets with the lateral size of approximately 100 ± 50 nm are conveniently fabricated by ultrasonic exfoliation of bulk g-C3N4 that derived from thermolysis of dicyandiamide after recrystallization. The nanosized g-C3N4 sheets exhibit excellently higher photocatalytic performance toward aflatoxin B1 (AFB1) degradation under visible light than that of bulk g-C3N4. And the enhanced photocatalytic mechanisms are mainly due to the better photogenerated charges separation and larger surface area of nanosized g-C3N4 sheets, which are systematically confirmed by physicochemical and photoelectric characterization. Through identification of products structure, it is found that the initial photodegradation intermediate (C17H14O7) or main products (C14H16O4 and C12H10O4) of AFB1 after 2 h irradiation over bulk g-C3N4 and nanosized g-C3N4 sheets are different, which may be attributed to the photoactivities and number of active groups from both catalysts during photoreaction. Therefore, this work may provide referential information for preparation of inerratic nanosized sheets and understand the related photodegradation mechanism of aflatoxin B1.
Jin Mao; Liangxiao Zhang; Huiting Wang; Qi Zhang; Wen Zhang; Peiwu Li. Facile fabrication of nanosized graphitic carbon nitride sheets with efficient charge separation for mitigation of toxic pollutant. Chemical Engineering Journal 2018, 342, 30 -40.
AMA StyleJin Mao, Liangxiao Zhang, Huiting Wang, Qi Zhang, Wen Zhang, Peiwu Li. Facile fabrication of nanosized graphitic carbon nitride sheets with efficient charge separation for mitigation of toxic pollutant. Chemical Engineering Journal. 2018; 342 ():30-40.
Chicago/Turabian StyleJin Mao; Liangxiao Zhang; Huiting Wang; Qi Zhang; Wen Zhang; Peiwu Li. 2018. "Facile fabrication of nanosized graphitic carbon nitride sheets with efficient charge separation for mitigation of toxic pollutant." Chemical Engineering Journal 342, no. : 30-40.
Adulteration of edible oils has attracted attention from more researchers and consumers in recent years. Complex multispecies adulteration is a commonly used strategy to mask the traditional adulteration detection methods. Most of the researchers were only concerned about single targeted adulterants, however, it was difficult to identify complex multispecies adulteration or untargeted adulterants. To detect adulteration of edible oil, identification of characteristic markers of adulterants was proposed to be an effective method, which could provide a solution for multispecies adulteration detection. In this study, a simple method of multispecies adulteration detection for camellia oil (adulterated with soybean oil, peanut oil, rapeseed oil) was developed by quantifying chemical markers including four isoflavones, trans-resveratrol and sinapic acid, which used liquid chromatography tandem mass spectrometry (LC-MS/MS) combined with solid phase extraction (SPE). In commercial camellia oil, only two of them were detected of daidzin with the average content of 0.06 ng/g while other markers were absent. The developed method was highly sensitive as the limits of detection (LODs) ranged from 0.02 ng/mL to 0.16 ng/mL and the mean recoveries ranged from 79.7% to 113.5%, indicating that this method was reliable to detect potential characteristic markers in edible oils. Six target compounds for pure camellia oils, soybean oils, peanut oils and rapeseed oils had been analyzed to get the results. The validation results indicated that this simple and rapid method was successfully employed to determine multispecies adulteration of camellia oil adulterated with soybean, peanut and rapeseed oils.
Xinjing Dou; Jin Mao; Liangxiao Zhang; Huali Xie; Lin Chen; Li Yu; Fei Ma; Xiupin Wang; Qi Zhang; Peiwu Li. Multispecies Adulteration Detection of Camellia Oil by Chemical Markers. Molecules 2018, 23, 241 .
AMA StyleXinjing Dou, Jin Mao, Liangxiao Zhang, Huali Xie, Lin Chen, Li Yu, Fei Ma, Xiupin Wang, Qi Zhang, Peiwu Li. Multispecies Adulteration Detection of Camellia Oil by Chemical Markers. Molecules. 2018; 23 (2):241.
Chicago/Turabian StyleXinjing Dou; Jin Mao; Liangxiao Zhang; Huali Xie; Lin Chen; Li Yu; Fei Ma; Xiupin Wang; Qi Zhang; Peiwu Li. 2018. "Multispecies Adulteration Detection of Camellia Oil by Chemical Markers." Molecules 23, no. 2: 241.
A highly sensitive aptasensor for aflatoxin M1 (AFM1) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method and exhibited broad and strong absorption in the whole ultraviolet-visible (UV-Vis) range. The strong coordination interaction between nitrogen functional groups of the AFM1 aptamer and PdNPs brought FAM and PdNPs in close proximity, which resulted in the fluorescence quenching of FAM to a maximum extent of 95%. The non-specific fluorescence quenching caused by PdNPs towards fluorescein was negligible. After the introduction of AFM1 into the FAM-AFM1 aptamer-PdNPs FRET system, the AFM1 aptamer preferentially combined with AFM1 accompanied by conformational change, which greatly weakened the coordination interaction between the AFM1 aptamer and PdNPs. Thus, fluorescence recovery of FAM was observed and a linear relationship between the fluorescence recovery and the concentration of AFM1 was obtained in the range of 5–150 pg/mL in aqueous buffer with the detection limit of 1.5 pg/mL. AFM1 detection was also realized in milk samples with a linear detection range from 6 pg/mL to 150 pg/mL. The highly sensitive FRET aptasensor with simple configuration shows promising prospect in detecting a variety of food contaminants.
Hui Li; Daibin Yang; Peiwu Li; Qi Zhang; Wen Zhang; Xiaoxia Ding; Jin Mao; Jing Wu. Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk. Toxins 2017, 9, 318 .
AMA StyleHui Li, Daibin Yang, Peiwu Li, Qi Zhang, Wen Zhang, Xiaoxia Ding, Jin Mao, Jing Wu. Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk. Toxins. 2017; 9 (10):318.
Chicago/Turabian StyleHui Li; Daibin Yang; Peiwu Li; Qi Zhang; Wen Zhang; Xiaoxia Ding; Jin Mao; Jing Wu. 2017. "Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk." Toxins 9, no. 10: 318.
Aflatoxins, a group of extremely hazardous compounds because of their genotoxicity and carcinogenicity to human and animals, are commonly found in many tropical and subtropical regions. Ultraviolet (UV) irradiation is proven to be an effective method to reduce or detoxify aflatoxins. However, the degradation products of aflatoxins under UV irradiation and their safety or toxicity have not been clear in practical production such as edible oil industry. In this study, the degradation products of aflatoxin B1 (AFB1) in peanut oil were analyzed by Ultra Performance Liquid Chromatograph-Thermo Quadrupole Exactive Focus mass spectrometry/mass spectrometry (UPLC-TQEF-MS/MS). The high-resolution mass spectra reflected that two main products were formed after the modification of a double bond in the terminal furan ring and the fracture of the lactone ring, while the small molecules especially nitrogen-containing compound may have participated in the photochemical reaction. According to the above results, the possible photodegradation pathway of AFB1 in peanut oil is proposed. Moreover, the human embryo hepatocytes viability assay indicated that the cell toxicity of degradation products after UV irradiation was much lower than that of AFB1, which could be attributed to the breakage of toxicological sites. These findings can provide new information for metabolic pathways and the hazard assessment of AFB1 using UV detoxification.
Jin Mao; Bing He; Liangxiao Zhang; Peiwu Li; Qi Zhang; Xiaoxia Ding; Wen Zhang. A Structure Identification and Toxicity Assessment of the Degradation Products of Aflatoxin B1 in Peanut Oil under UV Irradiation. Toxins 2016, 8, 332 .
AMA StyleJin Mao, Bing He, Liangxiao Zhang, Peiwu Li, Qi Zhang, Xiaoxia Ding, Wen Zhang. A Structure Identification and Toxicity Assessment of the Degradation Products of Aflatoxin B1 in Peanut Oil under UV Irradiation. Toxins. 2016; 8 (11):332.
Chicago/Turabian StyleJin Mao; Bing He; Liangxiao Zhang; Peiwu Li; Qi Zhang; Xiaoxia Ding; Wen Zhang. 2016. "A Structure Identification and Toxicity Assessment of the Degradation Products of Aflatoxin B1 in Peanut Oil under UV Irradiation." Toxins 8, no. 11: 332.
This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G1, B1, G2 and B2 were 0.03, 0.07, 0.05 and 0.09 μg·kg−1, and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 μg·kg−1, respectively. The recoveries of aflatoxins in samples were 90.1%–104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 μg·kg−1.
Fei Ma; Ran Chen; Peiwu Li; Qi Zhang; Wen Zhang; Xiaofeng Hu. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules 2013, 18, 2222 -2235.
AMA StyleFei Ma, Ran Chen, Peiwu Li, Qi Zhang, Wen Zhang, Xiaofeng Hu. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules. 2013; 18 (2):2222-2235.
Chicago/Turabian StyleFei Ma; Ran Chen; Peiwu Li; Qi Zhang; Wen Zhang; Xiaofeng Hu. 2013. "Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products." Molecules 18, no. 2: 2222-2235.
Chemical contaminants in food have caused serious health issues in both humans and animals. Microarray technology is an advanced technique suitable for the analysis of chemical contaminates. In particular, immuno-microarray approach is one of the most promising methods for chemical contaminants analysis. The use of microarrays for the analysis of chemical contaminants is the subject of this review. Fabrication strategies and detection methods for chemical contaminants are discussed in detail. Application to the analysis of mycotoxins, biotoxins, pesticide residues, and pharmaceutical residues is also described. Finally, future challenges and opportunities are discussed.
Zhaowei Zhang; Peiwu Li; Xiaofeng Hu; Qi Zhang; Xiaoxia Ding; Wen Zhang. Microarray Technology for Major Chemical Contaminants Analysis in Food: Current Status and Prospects. Sensors 2012, 12, 9234 -9252.
AMA StyleZhaowei Zhang, Peiwu Li, Xiaofeng Hu, Qi Zhang, Xiaoxia Ding, Wen Zhang. Microarray Technology for Major Chemical Contaminants Analysis in Food: Current Status and Prospects. Sensors. 2012; 12 (7):9234-9252.
Chicago/Turabian StyleZhaowei Zhang; Peiwu Li; Xiaofeng Hu; Qi Zhang; Xiaoxia Ding; Wen Zhang. 2012. "Microarray Technology for Major Chemical Contaminants Analysis in Food: Current Status and Prospects." Sensors 12, no. 7: 9234-9252.