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Dr. Serhat Turkmen
University of Alabama at Birmingham

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Research Keywords & Expertise

0 Aquaculture
0 Lipid Metabolism
0 Polyunsaturated fatty acids
0 FADS
0 epigenetics of fish

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FADS
Aquaculture
Polyunsaturated fatty acids
Lipid Metabolism

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Journal article
Published: 17 February 2021 in Animals
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Genetic selection in gilthead seabream (GSB), Sparus aurata, has been undertaken to improve the growth, feed efficiency, fillet quality, skeletal deformities and disease resistance, but no study is available to delineate the effect of genetic selection for growth trait on GSB reproductive performance under mass spawning condition. In this study, high growth (HG) or low growth (LG) GSB broodstock were selected to evaluate the sex steroid hormones, sperm, egg quality and reproductive performance under different feeding regime of commercial diet or experimental broodstock diet containing either fish oil (FO) or vegetable oil (VO) based diet. Under commercial diet feeding phase, broodstock selected for either high growth or low growth did not show any significant changes in the egg production per kg female whereas egg viability percentage was positively (p = 0.014) improved by the high growth trait broodstock group. The experimental diet feeding results revealed that both growth trait and dietary fatty acid composition influenced the reproductive performance of GSB broodstock. In the experimental diet feeding phase, we observed high growth trait GSB males produced a higher number of sperm cells (p < 0.001) and also showed a higher sperm motility (p = 0.048) percentage. The viable egg and larval production per spawn per kg female were significantly improved by the broodstock selected for high growth trait and fed with fish oil-based diet. This present study results signifies that gilthead seabream broodstock selected on growth trait could have positive role in improvement of sperm and egg quality to produce viable progeny.

ACS Style

Shajahan Ferosekhan; Serhat Turkmen; Cathaysa Pérez-García; Hanlin Xu; Ana Gómez; Nazeemashahul Shamna; Juan Afonso; Grethe Rosenlund; Ramón Fontanillas; Anselmo Gracia; Marisol Izquierdo; Sadasivam Kaushik. Influence of Genetic Selection for Growth and Broodstock Diet n-3 LC-PUFA Levels on Reproductive Performance of Gilthead Seabream, Sparus aurata. Animals 2021, 11, 519 .

AMA Style

Shajahan Ferosekhan, Serhat Turkmen, Cathaysa Pérez-García, Hanlin Xu, Ana Gómez, Nazeemashahul Shamna, Juan Afonso, Grethe Rosenlund, Ramón Fontanillas, Anselmo Gracia, Marisol Izquierdo, Sadasivam Kaushik. Influence of Genetic Selection for Growth and Broodstock Diet n-3 LC-PUFA Levels on Reproductive Performance of Gilthead Seabream, Sparus aurata. Animals. 2021; 11 (2):519.

Chicago/Turabian Style

Shajahan Ferosekhan; Serhat Turkmen; Cathaysa Pérez-García; Hanlin Xu; Ana Gómez; Nazeemashahul Shamna; Juan Afonso; Grethe Rosenlund; Ramón Fontanillas; Anselmo Gracia; Marisol Izquierdo; Sadasivam Kaushik. 2021. "Influence of Genetic Selection for Growth and Broodstock Diet n-3 LC-PUFA Levels on Reproductive Performance of Gilthead Seabream, Sparus aurata." Animals 11, no. 2: 519.

Journal article
Published: 23 November 2020 in Animals
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Previous studies have shown that it is possible to increase the ability of marine fish to produce long-chain polyunsaturated fatty acid from their 18C precursors by nutritional programming or using broodstock with a higher fatty acyl desaturase 2 (fads2) expression. However, those studies failed to show the effect of these interventions on the expression of the fads2 gene in the developing egg. Moreover, there were no studies on the temporal expression of the fads2 during ontogeny in the gilthead sea bream (Sparus aurata). In order to determine the changes in expression of fads2 during ontogeny, gilthead sea bream broodstock with a high (HRO) or low (LRO) fads2 expression fed a diet previously used for nutritional programming, or a fish oil-based diet (LFO) were allowed to spawn. The samples were taken at the stages of spawning, morula, high blastula, gastrula, neurula, heart beating, hatch and 3 day-old first exogenous feeding larvae to determine fads2 expression throughout embryonic development. The results showed the presence of fads2 mRNA in the just spawned egg, denoting the maternal mRNA transfer to the developing oocyte. Later, fads2 expression increased after the neurula, from heart beating until 3-day-old larvae, denoting the transition from maternal to embryonic gene expression. In addition, the eggs obtained from broodstock with high fads2 expression showed a high docosahexaenoic acid content, which correlated with the downregulation of the fads2 expression found in the developing embryo and larvae. Finally, feeding with the nutritional programming diet with the partial replacement of fish oil by rapeseed oil did not affect the long chain polyunsaturated fatty acid (LC-PUFA) contents nor fads2 expression in the gilthead sea bream developing eggs.

ACS Style

Hanlin Xu; Shajahan Ferosekhan; Serhat Turkmen; Juan Manuel Afonso; María Jesús Zamorano; Marisol Izquierdo. Influence of Parental fatty acid desaturase 2 (fads2) Expression and Diet on Gilthead Seabream (Sparus aurata) Offspring fads2 Expression during Ontogenesis. Animals 2020, 10, 2191 .

AMA Style

Hanlin Xu, Shajahan Ferosekhan, Serhat Turkmen, Juan Manuel Afonso, María Jesús Zamorano, Marisol Izquierdo. Influence of Parental fatty acid desaturase 2 (fads2) Expression and Diet on Gilthead Seabream (Sparus aurata) Offspring fads2 Expression during Ontogenesis. Animals. 2020; 10 (11):2191.

Chicago/Turabian Style

Hanlin Xu; Shajahan Ferosekhan; Serhat Turkmen; Juan Manuel Afonso; María Jesús Zamorano; Marisol Izquierdo. 2020. "Influence of Parental fatty acid desaturase 2 (fads2) Expression and Diet on Gilthead Seabream (Sparus aurata) Offspring fads2 Expression during Ontogenesis." Animals 10, no. 11: 2191.

Journal article
Published: 19 July 2020 in Life
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The principle aim of this study is to elucidate the relationship between the fatty acid desaturase 2 gene (fads2) expression pattern in peripheral blood cells (PBCs) and liver of gilthead seabream (GSB), Sparus aurata broodstock in order to determine the possible use of fads2 expression as a potential biomarker for the selection of broodstock. This selection could be utilized for breeding programs aiming to improve reproduction, health, and nutritional status. Passive Integrated Transponder (PIT)-tagged GSB broodstock (Male-1.22 ± 0.20 kg; 44.8 ± 2 cm and female-2.36 ± 0.64 kg; 55.1 cm) were fed a diet containing low levels of fish meal and fish oil (EPA 2.5; DHA 1.7 and n-3 LC-PUFA 4.6% TFA) for one month. After the feeding period, fads2 expression in PBCs and liver of both male and female broodstock were highly significantly correlated (r = 0.89; p < 0.001). Additionally, in male broodstock, liver fads2 expression was significantly correlated (p < 0.05) to liver contents in 16:0 (r = 0.95; p = 0.04) and total saturates (r = 0.97; p = 0.03) as well as to 20:3n–6/20:2n–6 (r = 0.98; p = 0.02) a Fads2 product/precursor ratio. Overall, we found a positive and significant correlation between fads2 expression levels in the PBCs and liver of GSB broodstock. PBCs fads2 expression levels indicate a strong potential for utilization as a non-invasive method to select animals having increased fatty acid bioconversion capability, better able to deal with diets free of fish meal and fish oil.

ACS Style

Shajahan Ferosekhan; Serhat Turkmen; Hanlin Xu; Juan Manuel Afonso; Maria Jesus Zamorano; Sadasivam Kaushik; Marisol Izquierdo. The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet. Life 2020, 10, 117 .

AMA Style

Shajahan Ferosekhan, Serhat Turkmen, Hanlin Xu, Juan Manuel Afonso, Maria Jesus Zamorano, Sadasivam Kaushik, Marisol Izquierdo. The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet. Life. 2020; 10 (7):117.

Chicago/Turabian Style

Shajahan Ferosekhan; Serhat Turkmen; Hanlin Xu; Juan Manuel Afonso; Maria Jesus Zamorano; Sadasivam Kaushik; Marisol Izquierdo. 2020. "The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet." Life 10, no. 7: 117.

Journal article
Published: 01 January 2020 in Journal of Experimental Biology
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Environmental factors such as nutritional interventions during early developmental stages affect and establish long-term metabolic changes in all animals. Diets used during the spawning period causes a nutritional programming effect in offspring of gilthead sea bream and affects long-term metabolism. Studies showed modulation of genes such as fads2 which is considered to be a rate-limiting step in the synthesis of n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA). However, it is still unknown whether this adaptation is related to the presence of precursors or limitations in the pre-formed products, n-3 LC-PUFA, contained in the diets used during nutritional programming. This study investigates the combined effects of nutritional programming through broodstock diets during the spawning period and broodfish showing higher or lower fads2 expression levels in the blood after 1-month feeding with a diet containing high levels of plant protein sources and vegetable oils (VM/VO). Broodfish showing high fads2 expression had a noticeable improvement in the spawning quality parameters as well as on the growth of 6 months old offspring when challenged with a high VM/VO diet. Further, nutritional conditioning with 18:3n-3 rich diets had an adverse effect in comparison to progeny obtained from fish fed high fish meal and fish oil (FM/FO) diets, with a reduction in growth of juveniles. Improved growth of progeny from the high fads2 broodstock combined with similar muscle fatty acid profiles is an excellent option also for tailoring and increasing the flesh n-3 LC-PUFA levels to meet the recommended dietary allowances for human consumption.

ACS Style

Serhat Turkmen; Maria J. Zamorano; Hanlin Xu; Hipólito Fernández-Palacios; Lidia Robaina; Sadasivam Kaushik; Marisol Izquierdo. Parental LC-PUFA biosynthesis capacity and nutritional intervention with ALA affect performance of Sparus aurata progeny. Journal of Experimental Biology 2020, 1 .

AMA Style

Serhat Turkmen, Maria J. Zamorano, Hanlin Xu, Hipólito Fernández-Palacios, Lidia Robaina, Sadasivam Kaushik, Marisol Izquierdo. Parental LC-PUFA biosynthesis capacity and nutritional intervention with ALA affect performance of Sparus aurata progeny. Journal of Experimental Biology. 2020; ():1.

Chicago/Turabian Style

Serhat Turkmen; Maria J. Zamorano; Hanlin Xu; Hipólito Fernández-Palacios; Lidia Robaina; Sadasivam Kaushik; Marisol Izquierdo. 2020. "Parental LC-PUFA biosynthesis capacity and nutritional intervention with ALA affect performance of Sparus aurata progeny." Journal of Experimental Biology , no. : 1.

Journal article
Published: 11 December 2019 in International Journal of Molecular Sciences
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Polyunsaturated fatty acids (PUFA) in parental diets play a key role in regulating n-3 LC-PUFA metabolism of the offspring. However, it is not clear whether this metabolic regulation is driven by the precursors presented in the diet or by the parental ability to synthesize them. To elucidate this, broodstocks of gilthead sea bream with different blood expression levels of fads2, which encodes for the rate-limiting enzyme in the n-3 LC-PUFA synthesis pathway, were fed either a diet supplemented with alpha-linolenic acid (ALA, 18:3n-3) or a control diet. The progenies obtained from these four experimental groups were then challenged with a low LC-PUFA diet at the juvenile stage. Results showed that the offspring from parents with high fads2 expression presented higher growth and improved utilization of low n-3 LC-PUFA diets compared to the offspring from parents with low fads2 expression. Besides, an ALA-rich diet during the gametogenesis caused negative effects on the growth of the offspring. The epigenetic analysis demonstrated that methylation in the promoter of fads2 of the offspring was correlated with the parental fads2 expression levels and type of the broodstock diet.

ACS Style

Serhat Turkmen; Erick Perera; Maria J. Zamorano; Paula Simó-Mirabet; Hanlin Xu; Jaume Pérez-Sánchez; Marisol Izquierdo. Effects of Dietary Lipid Composition and Fatty Acid Desaturase 2 Expression in Broodstock Gilthead Sea Bream on Lipid Metabolism-Related Genes and Methylation of the fads2 Gene Promoter in Their Offspring. International Journal of Molecular Sciences 2019, 20, 6250 .

AMA Style

Serhat Turkmen, Erick Perera, Maria J. Zamorano, Paula Simó-Mirabet, Hanlin Xu, Jaume Pérez-Sánchez, Marisol Izquierdo. Effects of Dietary Lipid Composition and Fatty Acid Desaturase 2 Expression in Broodstock Gilthead Sea Bream on Lipid Metabolism-Related Genes and Methylation of the fads2 Gene Promoter in Their Offspring. International Journal of Molecular Sciences. 2019; 20 (24):6250.

Chicago/Turabian Style

Serhat Turkmen; Erick Perera; Maria J. Zamorano; Paula Simó-Mirabet; Hanlin Xu; Jaume Pérez-Sánchez; Marisol Izquierdo. 2019. "Effects of Dietary Lipid Composition and Fatty Acid Desaturase 2 Expression in Broodstock Gilthead Sea Bream on Lipid Metabolism-Related Genes and Methylation of the fads2 Gene Promoter in Their Offspring." International Journal of Molecular Sciences 20, no. 24: 6250.

Research paper
Published: 06 December 2019 in Epigenetics
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The aim of this study was to generate new knowledge on fish epigenetics, assessing the effects of linolenic acid (ALA) conditioning of broodstock in the offspring of the marine fish Sparus aurata. Attention was focused on gene organization, methylation signatures and gene expression patterns of fatty acid desaturase 2 (fads2) and stearoyl-CoA desaturase 1a (scd1a). Blat searches in the genomic IATS-CSIC database (www.nutrigroup-iats.org/seabreamdb) highlighted a conserved exon-intron organization, a conserved PUFA response region, and CG islands at the promoter regions of each gene. The analysed CpG positions in the fads2 promoter were mostly hypomethylated and refractory to broodstock nutrition. The same response was achieved after conditioning of juvenile fish to low water oxygen concentrations, thus methylation susceptibility at individual CpG sites seems to be stringently regulated in fish of different origin and growth trajectories. Conversely, the scd1a promoter was responsive to broodstock nutrition and the offspring of parents fed the ALA-rich diet shared an increased DNA-methylation, mainly in CpG sites neighbouring SP1 and HNF4α binding sites. Cytosine methylation at these sites correlated inversely with the hepatic scd1a expression of the offspring. Co-expression analyses supported that the HNF4α-dependent regulation of scd1a is affected by DNA methylation. The phenotypic output is a regulated liver fat deposition through changes in scd1 expression, which would also allow the preservation of fatty acid unsaturation levels in fish fed reduced levels of n-3 LC-PUFA. Collectively, these findings reveal a reliable mechanism by which parent’s nutrition can shape scd1a gene expression in the fish offspring.

ACS Style

Erick Perera; Serhat Turkmen; Paula Simó-Mirabet; Maria J. Zamorano; Hanlin Xu; Fernando Naya-Català; Marisol Izquierdo; Jaume Pérez-Sánchez. Stearoyl-CoA desaturase (scd1a) is epigenetically regulated by broodstock nutrition in gilthead sea bream (Sparus aurata). Epigenetics 2019, 15, 536 -553.

AMA Style

Erick Perera, Serhat Turkmen, Paula Simó-Mirabet, Maria J. Zamorano, Hanlin Xu, Fernando Naya-Català, Marisol Izquierdo, Jaume Pérez-Sánchez. Stearoyl-CoA desaturase (scd1a) is epigenetically regulated by broodstock nutrition in gilthead sea bream (Sparus aurata). Epigenetics. 2019; 15 (5):536-553.

Chicago/Turabian Style

Erick Perera; Serhat Turkmen; Paula Simó-Mirabet; Maria J. Zamorano; Hanlin Xu; Fernando Naya-Català; Marisol Izquierdo; Jaume Pérez-Sánchez. 2019. "Stearoyl-CoA desaturase (scd1a) is epigenetically regulated by broodstock nutrition in gilthead sea bream (Sparus aurata)." Epigenetics 15, no. 5: 536-553.

Journal article
Published: 01 November 2019 in Aquaculture
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ACS Style

Hanlin Xu; Serhat Turkmen; Simona Rimoldi; Genciana Terova; María Jesús Zamorano; Juan Manuel Afonso; Samira Sarih; Hipólito Fernández-Palacios; Marisol Izquierdo. Nutritional intervention through dietary vegetable proteins and lipids to gilthead sea bream (Sparus aurata) broodstock affects the offspring utilization of a low fishmeal/fish oil diet. Aquaculture 2019, 513, 1 .

AMA Style

Hanlin Xu, Serhat Turkmen, Simona Rimoldi, Genciana Terova, María Jesús Zamorano, Juan Manuel Afonso, Samira Sarih, Hipólito Fernández-Palacios, Marisol Izquierdo. Nutritional intervention through dietary vegetable proteins and lipids to gilthead sea bream (Sparus aurata) broodstock affects the offspring utilization of a low fishmeal/fish oil diet. Aquaculture. 2019; 513 ():1.

Chicago/Turabian Style

Hanlin Xu; Serhat Turkmen; Simona Rimoldi; Genciana Terova; María Jesús Zamorano; Juan Manuel Afonso; Samira Sarih; Hipólito Fernández-Palacios; Marisol Izquierdo. 2019. "Nutritional intervention through dietary vegetable proteins and lipids to gilthead sea bream (Sparus aurata) broodstock affects the offspring utilization of a low fishmeal/fish oil diet." Aquaculture 513, no. : 1.

Journal article
Published: 03 July 2019 in British Journal of Nutrition
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The present study investigated the effects of nutritional programming through parental feeding on offspring performance and expression of selected genes related to stress resistance in a marine teleost. Gilthead seabream broodstock were fed diets containing various fish oil (FO)/vegetable oil ratios to determine their effects on offspring performance along embryogenesis, larval development and juvenile on-growing periods. Increased substitution of dietary FO by linseed oil (LO) up to 80 % LO significantly reduced the total number of eggs produced by kg per female per spawn. Moreover, at 30 d after hatching, parental feeding with increasing LO up to 80 % led to up-regulation of the fatty acyl desaturase 2 gene (fads2) that was correlated with the increase in conversion rates of related PUFA. Besides, cyclo-oxygenase 2 (cox2) and TNF-α (tnf-α) gene expression was also up-regulated by the increase in LO in broodstock diets up to 60 or 80 %, respectively. When 4-month-old offspring were challenged with diets having different levels of FO, the lowest growth was found in juveniles from broodstock fed 100 % FO. An increase in LO levels in the broodstock diet up to 60LO raised LC-PUFA levels in the juveniles, regardless of the juvenile’s diet. The results showed that it is possible to nutritionally programme gilthead seabream offspring through the modification of the fatty acid profiles of parental diets to improve the growth performance of juveniles fed low FO diets, inducing long-term changes in PUFA metabolism with up-regulation offads2expression. The present study provided the first pieces of evidence of the up-regulation of immune system-related genes in the offspring of parents fed increased FO replacement by LO.

ACS Style

Serhat Turkmen; Carmen M. Hernández-Cruz; María J. Zamorano; Hipólito Fernández-Palacios; Daniel Montero; Juan M. Afonso; Marisol Izquierdo. Long-chain PUFA profiles in parental diets induce long-term effects on growth, fatty acid profiles, expression of fatty acid desaturase 2 and selected immune system-related genes in the offspring of gilthead seabream. British Journal of Nutrition 2019, 122, 25 -38.

AMA Style

Serhat Turkmen, Carmen M. Hernández-Cruz, María J. Zamorano, Hipólito Fernández-Palacios, Daniel Montero, Juan M. Afonso, Marisol Izquierdo. Long-chain PUFA profiles in parental diets induce long-term effects on growth, fatty acid profiles, expression of fatty acid desaturase 2 and selected immune system-related genes in the offspring of gilthead seabream. British Journal of Nutrition. 2019; 122 (1):25-38.

Chicago/Turabian Style

Serhat Turkmen; Carmen M. Hernández-Cruz; María J. Zamorano; Hipólito Fernández-Palacios; Daniel Montero; Juan M. Afonso; Marisol Izquierdo. 2019. "Long-chain PUFA profiles in parental diets induce long-term effects on growth, fatty acid profiles, expression of fatty acid desaturase 2 and selected immune system-related genes in the offspring of gilthead seabream." British Journal of Nutrition 122, no. 1: 25-38.

Original article
Published: 26 July 2018 in Aquaculture Research
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There is a need to find sustainable alternatives to fishmeal (FM) and fish oil (FO) in feed formulations to support the continued growth of aquaculture. FM is mostly produced from mass‐caught pelagic species, but the production has been relatively constant for several decades. The aim of this study was to investigate the potential of dietary krill meal (KM) inclusion as a sustainable alternative to FM. In view of that, a feeding trial with gilthead seabream juveniles was conducted to evaluate whether dietary KM at 3%, 6% and 9% inclusion improves growth performance in comparison with a control diet. At the end of the study, fish in the 9% KM group showed significantly higher body weight (32.76 g) compared with fish fed the control diet (30.30 g). Moreover, FM replacement by 9% KM indicated a reduction in the accumulation of lipid droplets in the hepatocytes and around the pancreatic islets. In summary, this study suggests that FM can be reduced in diets for seabream without negatively affecting growth performance, when KM is added. On the contrary, KM enhances gilthead seabream growth and reduces lipid accumulation and damage of hepatocytes, which will open an interesting innovation line to completely replace FM by alternative terrestrial protein sources and the partial inclusion of KM.

ACS Style

Reda Saleh; Lena Burri; Tibiabin Benitez-Santana; Serhat Turkmen; Pedro Luis Castro; Marisol Izquierdo. Dietary krill meal inclusion contributes to better growth performance of gilthead seabream juveniles. Aquaculture Research 2018, 49, 3289 -3295.

AMA Style

Reda Saleh, Lena Burri, Tibiabin Benitez-Santana, Serhat Turkmen, Pedro Luis Castro, Marisol Izquierdo. Dietary krill meal inclusion contributes to better growth performance of gilthead seabream juveniles. Aquaculture Research. 2018; 49 (10):3289-3295.

Chicago/Turabian Style

Reda Saleh; Lena Burri; Tibiabin Benitez-Santana; Serhat Turkmen; Pedro Luis Castro; Marisol Izquierdo. 2018. "Dietary krill meal inclusion contributes to better growth performance of gilthead seabream juveniles." Aquaculture Research 49, no. 10: 3289-3295.

Journal article
Published: 10 February 2017 in British Journal of Nutrition
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Nutrition during periconception and early development can modulate metabolic routes to prepare the offspring for adverse conditions through a process known as nutritional programming. In gilthead sea bream, replacement of fish oil (FO) with linseed oil (LO) in broodstock diets improves growth in the 4-month-old offspring challenged with low-FO and low-fishmeal (FM) diets for 1 month. The present study further investigated the effects of broodstock feeding on the same offspring when they were 16 months old and were challenged for a second time with the low-FM and low-FO diet for 2 months. The results showed that replacement of parental moderate-FO feeding with LO, combined with juvenile feeding at 4 months old with low-FM and low-FO diets, significantly (Pα-linolenic acid and linoleic acid in broodstock diets lead to a significant down-regulation of hepatic lipoprotein lipase (PPn-3, 18 : 3n-6, 20 : 5n-3, 22 : 6n-3 and 22 : 5n-6. Thus, this study demonstrated the long-term nutritional programming of gilthead sea bream through broodstock feeding, the effect of feeding a ‘reminder’ diet during juvenile stages to improve utilisation of low-FM/FO diets and fish growth as well as the regulation of gene expression along the fish’s life-cycle.

ACS Style

Serhat Turkmen; Maria J. Zamorano; Hipólito Fernández-Palacios; Carmen M. Hernández-Cruz; Daniel Montero; Lidia Robaina; Marisol Izquierdo. Parental nutritional programming and a reminder during juvenile stage affect growth, lipid metabolism and utilisation in later developmental stages of a marine teleost, the gilthead sea bream (Sparus aurata). British Journal of Nutrition 2017, 118, 500 -512.

AMA Style

Serhat Turkmen, Maria J. Zamorano, Hipólito Fernández-Palacios, Carmen M. Hernández-Cruz, Daniel Montero, Lidia Robaina, Marisol Izquierdo. Parental nutritional programming and a reminder during juvenile stage affect growth, lipid metabolism and utilisation in later developmental stages of a marine teleost, the gilthead sea bream (Sparus aurata). British Journal of Nutrition. 2017; 118 (7):500-512.

Chicago/Turabian Style

Serhat Turkmen; Maria J. Zamorano; Hipólito Fernández-Palacios; Carmen M. Hernández-Cruz; Daniel Montero; Lidia Robaina; Marisol Izquierdo. 2017. "Parental nutritional programming and a reminder during juvenile stage affect growth, lipid metabolism and utilisation in later developmental stages of a marine teleost, the gilthead sea bream (Sparus aurata)." British Journal of Nutrition 118, no. 7: 500-512.

Journal article
Published: 27 August 2015 in Aquaculture Research
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The concept of nutritional programming raises the interesting possibility of directing specific metabolic pathways or functions in juvenile fish, for example, to improve the use of substitutes to fishmeal and oil, and hence to promote sustainability in aquaculture. The aim of the study was to determine effects of early nutritional stimuli of gilthead seabream larvae and check if nutritional programming of gilthead sea bream is possible between 16 days post hatching (dph) and 26 dph. A trial was conducted to determine the effects of early nutritional stimuli of gilthead seabream larvae. Five experimental microdiets (pellet size <250 μm) were formulated containing five different proportions of a marine lipid source rich in long‐chain polyunsaturated fatty acids (LC‐PUFA) and two vegetable lipid sources rich in linolenic and linoleic acids. The results of this study demonstrate that dietary n‐3 LC‐PUFA levels increased larval growth and survival affecting Δ6 desaturase gen (fads2) expression and retinal neurons density. However, the high mortalities obtained along on‐growing in fish fed low n‐3 LC‐PUFA at 16 dph constrained the feasibility of nutritional programming of gilthead seabream during this late developmental window and needs to be further investigated.

ACS Style

Serhat Türkmen; Pedro Luis Castro; Maria Jose Caballero; Reda Saleh; Maria Jesus Zamorano; Jose Regidor; Marisol Izquierdo; Carmen María Hernández-Cruz. Nutritional stimuli of gilthead seabream (Sparus aurata) larvae by dietary fatty acids: effects on larval performance, gene expression and neurogenesis. Aquaculture Research 2015, 48, 202 -213.

AMA Style

Serhat Türkmen, Pedro Luis Castro, Maria Jose Caballero, Reda Saleh, Maria Jesus Zamorano, Jose Regidor, Marisol Izquierdo, Carmen María Hernández-Cruz. Nutritional stimuli of gilthead seabream (Sparus aurata) larvae by dietary fatty acids: effects on larval performance, gene expression and neurogenesis. Aquaculture Research. 2015; 48 (1):202-213.

Chicago/Turabian Style

Serhat Türkmen; Pedro Luis Castro; Maria Jose Caballero; Reda Saleh; Maria Jesus Zamorano; Jose Regidor; Marisol Izquierdo; Carmen María Hernández-Cruz. 2015. "Nutritional stimuli of gilthead seabream (Sparus aurata) larvae by dietary fatty acids: effects on larval performance, gene expression and neurogenesis." Aquaculture Research 48, no. 1: 202-213.

Journal article
Published: 28 April 2015 in Aquaculture
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The complete removal of fishmeal (FM) and fish oil (FO) is required to promote the sustainable development of aquaculture and for that, fast growing high quality fish that are fed without FM and FO are necessary. Early nutritional programming may allow the production of fish better adapted to utilize diets with vegetable meals (VM) and oils (VO). The main objective of this study was to research in the potential value of fatty acids as modulators of early nutritional programming in marine fish for a better utilization of VO/VM. For that purpose gilthead sea bream (Sparus aurata) broodstock were fed four different replacement levels of FO by linseed oil (LO) and their effect on fecundity and spawn quality, egg composition, Δ-6-desaturase (Δ6D) gene expression, progeny growth performance and their growth response to a challenge with diets low in FO and FM, but high in VO and VM. The results showed that feeding gilthead sea bream broodstock with high LO diets had very long-term effects on the progeny. Thus, FO replacement by LO up to 80–100% in broodstock diets for gilthead sea bream not only reduced fecundity and spawn quality, but also growth of 45 dah and 4-month-old juveniles, as well as Δ6D gene expression. However, when the 4 month-old juveniles were fed with a low FM and FO diet, even those from broodstock fed only 60% replacement of FO by LO showed a higher growth and feed utilization than juveniles from parents fed FO. These results demonstrate the interesting potential of early nutritional programming of marine fish by broodstock feeding to improve long-term performance of the progeny. Further studies are being conducted to determine optimum nutrient levels in the broodstock diets and the molecular mechanisms implied to develop effective nutritional intervention strategies for this species. This study demonstrates for the first time in fish the potential of broodstock nutrition to conduct early nutritional programming of culture fish for a better utilization of low fish meal and fish oil diets by the progeny, showing its effect not only during reproduction and larval development but also during on-growing.

ACS Style

M.S. Izquierdo; S. Turkmen; D. Montero; M.J. Zamorano; J.M. Afonso; Vasileios Karalazos; H. Fernández-Palacios. Nutritional programming through broodstock diets to improve utilization of very low fishmeal and fish oil diets in gilthead sea bream. Aquaculture 2015, 449, 18 -26.

AMA Style

M.S. Izquierdo, S. Turkmen, D. Montero, M.J. Zamorano, J.M. Afonso, Vasileios Karalazos, H. Fernández-Palacios. Nutritional programming through broodstock diets to improve utilization of very low fishmeal and fish oil diets in gilthead sea bream. Aquaculture. 2015; 449 ():18-26.

Chicago/Turabian Style

M.S. Izquierdo; S. Turkmen; D. Montero; M.J. Zamorano; J.M. Afonso; Vasileios Karalazos; H. Fernández-Palacios. 2015. "Nutritional programming through broodstock diets to improve utilization of very low fishmeal and fish oil diets in gilthead sea bream." Aquaculture 449, no. : 18-26.

Journal article
Published: 31 January 2012 in Journal of Thermal Biology
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ACS Style

Nafiye Dülger; Metin Kumlu; Serhat Türkmen; Abdullatif Ölçülü; Orhan Tufan Eroldogan; Hatice Asuman Yilmaz; Noyan Öçal. Thermal tolerance of European Sea Bass (Dicentrarchus labrax) juveniles acclimated to three temperature levels. Journal of Thermal Biology 2012, 37, 79 -82.

AMA Style

Nafiye Dülger, Metin Kumlu, Serhat Türkmen, Abdullatif Ölçülü, Orhan Tufan Eroldogan, Hatice Asuman Yilmaz, Noyan Öçal. Thermal tolerance of European Sea Bass (Dicentrarchus labrax) juveniles acclimated to three temperature levels. Journal of Thermal Biology. 2012; 37 (1):79-82.

Chicago/Turabian Style

Nafiye Dülger; Metin Kumlu; Serhat Türkmen; Abdullatif Ölçülü; Orhan Tufan Eroldogan; Hatice Asuman Yilmaz; Noyan Öçal. 2012. "Thermal tolerance of European Sea Bass (Dicentrarchus labrax) juveniles acclimated to three temperature levels." Journal of Thermal Biology 37, no. 1: 79-82.

Journal article
Published: 13 September 2011 in Aquaculture Research
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ACS Style

Serhat Türkmen; Orhan Tufan Eroldogan; Hatice Asuman Yılmaz; Abdüllatif Ölçülü; Gül Ayten Kiriş Inan; Zeynep Erçen; Nazmi Tekelioğlu. Compensatory growth response of European sea bass (Dicentrarchus labraxL.) under cycled starvation and restricted feeding rate. Aquaculture Research 2011, 43, 1643 -1650.

AMA Style

Serhat Türkmen, Orhan Tufan Eroldogan, Hatice Asuman Yılmaz, Abdüllatif Ölçülü, Gül Ayten Kiriş Inan, Zeynep Erçen, Nazmi Tekelioğlu. Compensatory growth response of European sea bass (Dicentrarchus labraxL.) under cycled starvation and restricted feeding rate. Aquaculture Research. 2011; 43 (11):1643-1650.

Chicago/Turabian Style

Serhat Türkmen; Orhan Tufan Eroldogan; Hatice Asuman Yılmaz; Abdüllatif Ölçülü; Gül Ayten Kiriş Inan; Zeynep Erçen; Nazmi Tekelioğlu. 2011. "Compensatory growth response of European sea bass (Dicentrarchus labraxL.) under cycled starvation and restricted feeding rate." Aquaculture Research 43, no. 11: 1643-1650.

Journal article
Published: 31 August 2010 in Journal of Thermal Biology
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Critical thermal minima (CTMin) and maxima (CTMax) values were determined for the Pacific white shrimp Litopenaeus vannamei post-larvae and juveniles at four different acclimation temperatures (15, 20, 25, and 30 °C). The CTMin of shrimp at these acclimation temperatures were 7.82, 8.95, 9.80, and 10.96 °C for post-larvae and 7.50, 8.20, 10.20, and 10.80 °C for juveniles, respectively, at 1 °C h−1 cooling rate. The CTMax values were 35.65, 38.13, 39.91, and 42.00 °C for post-larvae and 35.94, 38.65, 40.30, and 42.20 °C for juveniles at the respective acclimation temperatures. Both acclimation temperature and size of the shrimp affected CTMin values of L. vannamei (P0.01). The area of the thermal tolerance polygon over four acclimation temperatures (15, 20, 25, and 30 °C) for the post-larvae of L. vannamei was calculated to be 434.94 °C2. The acclimation response ratio (ARR) values were high ranging from 0.35 to 0.44 for both post-larvae and juveniles. L. vannamei appears to be more sensitive to low temperatures than other penaeid species and its cold tolerance zone ranged from 7.5 to 11 °C. In successful aquaculture temperature must never fall below 12 °C to prevent mortalities. Upper thermal tolerance is less of a problem as in most subtropical regions maximum water temperature rarely exceeds 34 °C, but care should be given if shallow ponds with low water renewal rate are being used.

ACS Style

Metin Kumlu; Serhat Türkmen; Mehmet Kumlu. Thermal tolerance of Litopenaeus vannamei (Crustacea: Penaeidae) acclimated to four temperatures. Journal of Thermal Biology 2010, 35, 305 -308.

AMA Style

Metin Kumlu, Serhat Türkmen, Mehmet Kumlu. Thermal tolerance of Litopenaeus vannamei (Crustacea: Penaeidae) acclimated to four temperatures. Journal of Thermal Biology. 2010; 35 (6):305-308.

Chicago/Turabian Style

Metin Kumlu; Serhat Türkmen; Mehmet Kumlu. 2010. "Thermal tolerance of Litopenaeus vannamei (Crustacea: Penaeidae) acclimated to four temperatures." Journal of Thermal Biology 35, no. 6: 305-308.

Journal article
Published: 31 August 2010 in Journal of Thermal Biology
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Critical thermal minima (CTMin) were determined for the Pacific white shrimp Litopenaeus vannamei juveniles from four different acclimation temperatures (15, 20, 25, and 30 °C) and salinities (10‰, 20‰, 30‰, and 40‰). The lowest and highest CTMin of shrimp ranged between 7.2 °C at 15 °C/30‰ and 11.44 °C at 30 °C/20‰ at the cooling rate of 1 °C h−1. Acclimation temperature and salinity, as well as the interaction of both parameters, had significant effects on the CTMin values of L. vannamei (P<0.01). Yet, the results showed a much more profound effect of temperature on low thermal tolerance of juveniles. Only 40‰ salinity had an influence on the CTMin values (P<0.01). As the acclimation temperature was lowered from 30 to 15 °C thermal tolerance of the shrimp significantly increased by 3.25–4.14 °C. The acclimation response ratio (ARR) of the Pacific white shrimp exposed to different combinations of salinity and temperature ranged between 0.25 and 0.27. When this species is farmed in sub-tropical regions, its pond water temperature in the over-wintering facilities (regardless of the water salinity level) must never fall below 12 °C throughout the cold season to prevent mortalities.

ACS Style

Metin Kumlu; Mehmet Kumlu; Serhat Turkmen. Combined effects of temperature and salinity on critical thermal minima of pacific white shrimp Litopenaeus vannamei (Crustacea: Penaeidae). Journal of Thermal Biology 2010, 35, 302 -304.

AMA Style

Metin Kumlu, Mehmet Kumlu, Serhat Turkmen. Combined effects of temperature and salinity on critical thermal minima of pacific white shrimp Litopenaeus vannamei (Crustacea: Penaeidae). Journal of Thermal Biology. 2010; 35 (6):302-304.

Chicago/Turabian Style

Metin Kumlu; Mehmet Kumlu; Serhat Turkmen. 2010. "Combined effects of temperature and salinity on critical thermal minima of pacific white shrimp Litopenaeus vannamei (Crustacea: Penaeidae)." Journal of Thermal Biology 35, no. 6: 302-304.