This page has only limited features, please log in for full access.
In Brazil, antivenom for snakebite is currently formulated in liquid form and requires storage at 4 °C. Here, a new freeze-dried trivalent antivenom, which would enable cold-chain free storage, was determined to have efficacy in neutralizing the biological activities of Bothrops atrox venoms from Manaus (Brazil) and Leticia (Colombia), exhibiting an efficacy similar to those of currently available liquid Bothrops antivenoms. These results indicate that freeze-dried trivalent antivenom may be beneficial for applications in the Brazilian and Colombian Amazon regions.
Emiro G. Muniz; Sâmella S. Oliveira; Maria das Dores N. Noronha; Maria Das Graças G. Saraiva; Ida S. Sano-Martins. Use of freeze-dried trivalent antivenom to neutralize the toxic activities of Bothrops atrox snake venoms from the Amazon. Toxicon 2021, 200, 19 -22.
AMA StyleEmiro G. Muniz, Sâmella S. Oliveira, Maria das Dores N. Noronha, Maria Das Graças G. Saraiva, Ida S. Sano-Martins. Use of freeze-dried trivalent antivenom to neutralize the toxic activities of Bothrops atrox snake venoms from the Amazon. Toxicon. 2021; 200 ():19-22.
Chicago/Turabian StyleEmiro G. Muniz; Sâmella S. Oliveira; Maria das Dores N. Noronha; Maria Das Graças G. Saraiva; Ida S. Sano-Martins. 2021. "Use of freeze-dried trivalent antivenom to neutralize the toxic activities of Bothrops atrox snake venoms from the Amazon." Toxicon 200, no. : 19-22.
We characterized the hemorrhagic, coagulant and defibrinogenant activities of Lachesis muta venom and evaluated the capacity of the Brazilian antivenoms in neutralizing these activities. The hemorrhagic activity of L. muta venom was similarly neutralized by Bothrops, Bothrops-Lachesis and Bothrops-Crotalus antivenoms. The coagulant and defibrinogenant activities were better neutralized by the Bothrops-Lachesis antivenom. Bothrops-Crotalus antivenom also neutralized these activities, indicating that it can be an alternative to treat Lachesis envenomations when Bothrops-Lachesis antivenom is unavailable.
Emiro G. Muniz; Maria das Dores N. Noronha; Maria Das Graças G. Saraiva; Wuelton Marcelo Monteiro; Sâmella S. Oliveira. Neutralization of hemostatic disorders induced by Lachesis muta venom using Brazilian antivenoms. Toxicon 2020, 191, 44 -47.
AMA StyleEmiro G. Muniz, Maria das Dores N. Noronha, Maria Das Graças G. Saraiva, Wuelton Marcelo Monteiro, Sâmella S. Oliveira. Neutralization of hemostatic disorders induced by Lachesis muta venom using Brazilian antivenoms. Toxicon. 2020; 191 ():44-47.
Chicago/Turabian StyleEmiro G. Muniz; Maria das Dores N. Noronha; Maria Das Graças G. Saraiva; Wuelton Marcelo Monteiro; Sâmella S. Oliveira. 2020. "Neutralization of hemostatic disorders induced by Lachesis muta venom using Brazilian antivenoms." Toxicon 191, no. : 44-47.
Bleeding is a common hemostatic disorder that occurs in Bothrops envenomations. We evaluated the changes in coagulation, fibrinolysis components, and platelets in Bothrops atrox envenomations with bleeding. This is an observational study with B. atrox snakebite patients (n = 100) treated in Manaus, Brazilian Amazon. Bleeding was recorded on admission and during hospitalization. We found that the platelet count in our patients presented a weak correlation to tissue factor, factor II, and plasminogen. Tissue factor presented weak correlation to factor V, II, D-dimer, plasminogen, alpha 2-antiplasmin, and moderate correlation to fibrinogen and fibrin/fibrinogen degradation product (FDP). Patients with systemic bleeding (n = 20) presented low levels of factor V, II, fibrinogen, plasminogen, and alpha 2-antiplasmin, and high levels of tissue factor and FDP compared to those without bleeding. Patients with only local bleeding (n = 41) and without bleeding showed similar levels of hemostatic factors. Thrombocytopenia was observed mainly in patients with systemic bleeding and increased levels of serum venom. No association was found between venom levels and systemic bleeding, or between venom levels and clinical severity of envenomation. This is the first report that shows the participation of the extrinsic coagulation pathway in the consumption coagulopathy of B. atrox envenomations with systemic bleeding due to tissue factor release.
Sâmella S. Oliveira; Eliane C. Alves; Alessandra S. Santos; Elizandra F. Nascimento; João T. Pereira; Iran M. Silva; Jacqueline A. G. Sachett; Lybia S. Sarraff; Luciana Freitas-De-Sousa; Mônica Colombini; Hedylamar O. Marques; Marcus V. G. Lacerda; Marco Sartim; Ana Moura-Da-Silva; Luiz L. Ferreira; Ida S. Sano-Martins; Wuelton M. Monteiro. Bleeding Disorders in Bothrops atrox Envenomations in the Brazilian Amazon: Participation of Hemostatic Factors and the Impact of Tissue Factor. Toxins 2020, 12, 554 .
AMA StyleSâmella S. Oliveira, Eliane C. Alves, Alessandra S. Santos, Elizandra F. Nascimento, João T. Pereira, Iran M. Silva, Jacqueline A. G. Sachett, Lybia S. Sarraff, Luciana Freitas-De-Sousa, Mônica Colombini, Hedylamar O. Marques, Marcus V. G. Lacerda, Marco Sartim, Ana Moura-Da-Silva, Luiz L. Ferreira, Ida S. Sano-Martins, Wuelton M. Monteiro. Bleeding Disorders in Bothrops atrox Envenomations in the Brazilian Amazon: Participation of Hemostatic Factors and the Impact of Tissue Factor. Toxins. 2020; 12 (9):554.
Chicago/Turabian StyleSâmella S. Oliveira; Eliane C. Alves; Alessandra S. Santos; Elizandra F. Nascimento; João T. Pereira; Iran M. Silva; Jacqueline A. G. Sachett; Lybia S. Sarraff; Luciana Freitas-De-Sousa; Mônica Colombini; Hedylamar O. Marques; Marcus V. G. Lacerda; Marco Sartim; Ana Moura-Da-Silva; Luiz L. Ferreira; Ida S. Sano-Martins; Wuelton M. Monteiro. 2020. "Bleeding Disorders in Bothrops atrox Envenomations in the Brazilian Amazon: Participation of Hemostatic Factors and the Impact of Tissue Factor." Toxins 12, no. 9: 554.
Bothrops snakebites usually present systemic bleeding, and the clinical–epidemiological and laboratorial factors associated with the development of this manifestation are not well established. In this study, we assessed the prevalence of Bothrops snakebites with systemic bleeding reported at the Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, in Manaus, Amazonas State, Brazil, and the clinical–epidemiological and laboratorial factors associated with systemic bleeding. This is an observational, cross-sectional study carried out between August, 2013 and July, 2016. Patients who developed systemic bleeding on admission or during hospitalization were considered cases, and those with non-systemic bleeding were included in the control group. Systemic bleeding was observed in 63 (15.3%) of the 442 Bothrops snakebites evaluated. Bothrops snakebites mostly occurred in males (78.2%), in rural areas (89.0%) and in the age group of 11 to 30 years old (40.4%). It took most of the patients (59.8%) less than 3 h to receive medical assistance. Unclottable blood (AOR = 3.11 (95% CI = 1.53 to 6.31; p = 0.002)) and thrombocytopenia (AOR = 4.52 (95% CI = 2.03 to 10.09; p < 0.001)) on admission were independently associated with systemic bleeding during hospitalization. These hemostatic disorders on admission increase the chances of systemic bleeding during hospitalization. Prospective studies are needed to clarify the pathophysiology of systemic bleeding in Bothrops snakebites in the Amazon region.
Sâmella S. Oliveira; Eliane C. Alves; Alessandra S. Santos; João Pedro T. Pereira; Lybia Kássia S. Sarraff; Elizandra F. Nascimento; José Diego De-Brito-Sousa; Vanderson S. Sampaio; Marcus V.G. Lacerda; Jacqueline A.G. Sachett; Ida S. Sano-Martins; Wuelton M. Monteiro. Factors Associated with Systemic Bleeding in Bothrops Envenomation in a Tertiary Hospital in the Brazilian Amazon. Toxins 2019, 11, 22 .
AMA StyleSâmella S. Oliveira, Eliane C. Alves, Alessandra S. Santos, João Pedro T. Pereira, Lybia Kássia S. Sarraff, Elizandra F. Nascimento, José Diego De-Brito-Sousa, Vanderson S. Sampaio, Marcus V.G. Lacerda, Jacqueline A.G. Sachett, Ida S. Sano-Martins, Wuelton M. Monteiro. Factors Associated with Systemic Bleeding in Bothrops Envenomation in a Tertiary Hospital in the Brazilian Amazon. Toxins. 2019; 11 (1):22.
Chicago/Turabian StyleSâmella S. Oliveira; Eliane C. Alves; Alessandra S. Santos; João Pedro T. Pereira; Lybia Kássia S. Sarraff; Elizandra F. Nascimento; José Diego De-Brito-Sousa; Vanderson S. Sampaio; Marcus V.G. Lacerda; Jacqueline A.G. Sachett; Ida S. Sano-Martins; Wuelton M. Monteiro. 2019. "Factors Associated with Systemic Bleeding in Bothrops Envenomation in a Tertiary Hospital in the Brazilian Amazon." Toxins 11, no. 1: 22.
Snake envenomation is a major public health problem in Brazil. Systemic complications that may arise from snakebites are mainly related to coagulopathy. The Lee–White clotting time (LWCT) is a simple and inexpensive test and available even in remote health facilities. However, the diagnostic value of such test needs to be evaluated to accurately diagnose coagulopathy in the clinical practice. This study aimed to assess the reliability of the LWCT performed in hospital routine to diagnose venom-induced coagulopathy. We studied 186 patients admitted at the Tropical Medicine Foundation Dr. Heitor Vieira Dourado in Manaus, Amazonas, Brazil, with Bothrops envenomation diagnosis. At admission, blood samples were collected for performing LWCT and the concentration of fibrinogen. Sensitivity, specificity, positive predictive value, negative predictive value, likelihood ratios, diagnostic odds ratio, and accuracy were calculated with 95% confidence intervals. From the total, 85.5% had hypofibrinogenemia. The sensitivity of the LWCT to the diagnosis of hypofibrinogenemia was 78.0% and the specificity 40.7%. The accuracy of the test was 72.6%, and patients with a prolonged LWCT had 2.4 higher odds of developing hypofibrinogenemia. In addition, the LWCT was also compared with venom antigen levels and systemic hemorrhage. The LWCT showed moderate sensitivity to detect consumption coagulopathy and constitutes a valuable tool for the diagnosis of Bothrops snake envenomation and indication of antivenom therapy.
Jose Diego De Brito Sousa; Jacqueline Almeida Gonçalves Sachett; Sâmella Silva De Oliveira; Iran Mendonça-Da-Silva; Hedylamar Oliveira Marques; Marcus Vinicius Guimarães De Lacerda; Hui Wen Fan; Wuelton Marcelo Monteiro. Accuracy of the Lee–White Clotting Time Performed in the Hospital Routine to Detect Coagulopathy in Bothrops atrox Envenomation. The American Journal of Tropical Medicine and Hygiene 2018, 98, 1547 -1551.
AMA StyleJose Diego De Brito Sousa, Jacqueline Almeida Gonçalves Sachett, Sâmella Silva De Oliveira, Iran Mendonça-Da-Silva, Hedylamar Oliveira Marques, Marcus Vinicius Guimarães De Lacerda, Hui Wen Fan, Wuelton Marcelo Monteiro. Accuracy of the Lee–White Clotting Time Performed in the Hospital Routine to Detect Coagulopathy in Bothrops atrox Envenomation. The American Journal of Tropical Medicine and Hygiene. 2018; 98 (5):1547-1551.
Chicago/Turabian StyleJose Diego De Brito Sousa; Jacqueline Almeida Gonçalves Sachett; Sâmella Silva De Oliveira; Iran Mendonça-Da-Silva; Hedylamar Oliveira Marques; Marcus Vinicius Guimarães De Lacerda; Hui Wen Fan; Wuelton Marcelo Monteiro. 2018. "Accuracy of the Lee–White Clotting Time Performed in the Hospital Routine to Detect Coagulopathy in Bothrops atrox Envenomation." The American Journal of Tropical Medicine and Hygiene 98, no. 5: 1547-1551.
Although important efforts were carried out during the past decades in Brazil to understand and control snakebite envenomings, important gaps remain for the fulfillment of these goals, particularly in the Amazon region. Bothrops atrox is the most important venomous snake in the Brazilian Amazon, causing 80–90% of the snake envenomings in the region. In the Brazilian Amazon, Bothrops envenoming shows pain, swelling, regional lymphadenopathy, ecchymosis, blistering, and necrosis as the most common local clinical manifestations. Secondary bacterial infections were observed in around 40% of the Bothrops snakebites. Spontaneous systemic bleeding and acute renal failure are common systemic complications after Bothrops envenomings. It is difficult for riverine and indigenous populations to reach health centers for treatment of snakebites. As a result, the number of cases detected officially is probably underestimated. Current antivenoms (AVs) require conservation in adequate facilities, which are not always available in remote settings. In addition, training of multidisciplinary teams is not always appropriate for indigenous health services regarding AV administration, side effect management, and case monitoring and surveillance. Although clinical research related to venomous animal injuries has increased, most publications are based on case reports and lack methodological rigor. Moreover, outcome definitions, such as severity ranking criteria, were empirically established, making the results even less generalizable. Clinical research from hospital-based studies and community observational studies are needed. In addition to all the above recommendations, the importance of international cooperative efforts toward the control of these neglected health problems through international partnerships, namely, with other Amazonian countries, is highlighted.
Sâmella Silva de Oliveira; Vanderson De Souza Sampaio; Jacqueline De Almeida Gonçalves Sachett; Eliane Campos Alves; Vanessa Costa da Silva; João Arthur Alcântara de Lima; Iran Mendonça da Silva; Luiz Carlos De Lima Ferreira; Paulo Bernarde; Hui Wen Fan; Marcus Vinicius Guimarães de Lacerda; Wuelton Marcelo Monteiro. Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives. Toxins and Drug Discovery 2018, 73 -99.
AMA StyleSâmella Silva de Oliveira, Vanderson De Souza Sampaio, Jacqueline De Almeida Gonçalves Sachett, Eliane Campos Alves, Vanessa Costa da Silva, João Arthur Alcântara de Lima, Iran Mendonça da Silva, Luiz Carlos De Lima Ferreira, Paulo Bernarde, Hui Wen Fan, Marcus Vinicius Guimarães de Lacerda, Wuelton Marcelo Monteiro. Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives. Toxins and Drug Discovery. 2018; ():73-99.
Chicago/Turabian StyleSâmella Silva de Oliveira; Vanderson De Souza Sampaio; Jacqueline De Almeida Gonçalves Sachett; Eliane Campos Alves; Vanessa Costa da Silva; João Arthur Alcântara de Lima; Iran Mendonça da Silva; Luiz Carlos De Lima Ferreira; Paulo Bernarde; Hui Wen Fan; Marcus Vinicius Guimarães de Lacerda; Wuelton Marcelo Monteiro. 2018. "Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives." Toxins and Drug Discovery , no. : 73-99.
Although important efforts were carried out during the past decades in Brazil to understand and control snakebite envenomings, important gaps remain for the fulfillment of these goals, particularly in the Amazon region. Bothrops atrox is the most important venomous snake in the Brazilian Amazon, causing 80–90 % of the snake envenomings in the region. In the Brazilian Amazon, Bothrops envenoming shows pain, swelling, regional lymphadenopathy, ecchymosis, blistering, and necrosis as the most common local clinical manifestations. Secondary bacterial infections were observed in around 40 % of the Bothrops snakebites. Spontaneous systemic bleeding and acute renal failure are common systemic complications after Bothrops envenomings. It is difficult for riverine and indigenous populations to reach health centers for treatment of snakebites. As a result, the number of cases detected officially is probably underestimated. Current antivenoms (AVs) require conservation in adequate facilities, which are not always available in remote settings. In addition, training of multidisciplinary teams is not always appropriate for indigenous health services regarding AV administration, side effect management, and case monitoring and surveillance. Although clinical research related to venomous animal injuries has increased, most publications are based on case reports and lack methodological rigor. Moreover, outcome definitions, such as severity ranking criteria, were empirically established, making the results even less generalizable. Clinical research from hospital-based studies and community observational studies are needed. In addition to all the above recommendations, the importance of international cooperative efforts toward the control of these neglected health problems through international partnerships, namely, with other Amazonian countries, is highlighted.
Sâmella Silva De Oliveira; Vanderson De Souza Sampaio; Jacqueline De Almeida Gonçalves Sachett; Eliane Campos Alves; Vanessa Costa Da Silva; João Arthur Alcântara De Lima; Iran Mendonça Da Silva; Luiz Carlos De Lima Ferreira; Paulo Bernarde; Hui Wen Fan; Marcus Vinicius Guimarães De Lacerda; Wuelton Marcelo Monteiro. Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives. Toxins and Drug Discovery 2017, 1 -22.
AMA StyleSâmella Silva De Oliveira, Vanderson De Souza Sampaio, Jacqueline De Almeida Gonçalves Sachett, Eliane Campos Alves, Vanessa Costa Da Silva, João Arthur Alcântara De Lima, Iran Mendonça Da Silva, Luiz Carlos De Lima Ferreira, Paulo Bernarde, Hui Wen Fan, Marcus Vinicius Guimarães De Lacerda, Wuelton Marcelo Monteiro. Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives. Toxins and Drug Discovery. 2017; ():1-22.
Chicago/Turabian StyleSâmella Silva De Oliveira; Vanderson De Souza Sampaio; Jacqueline De Almeida Gonçalves Sachett; Eliane Campos Alves; Vanessa Costa Da Silva; João Arthur Alcântara De Lima; Iran Mendonça Da Silva; Luiz Carlos De Lima Ferreira; Paulo Bernarde; Hui Wen Fan; Marcus Vinicius Guimarães De Lacerda; Wuelton Marcelo Monteiro. 2017. "Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives." Toxins and Drug Discovery , no. : 1-22.
Although important efforts were carried out during the past decades in Brazil to understand and control snakebite envenomings, important gaps remain for the fulfillment of these goals, particularly in the Amazon region. Bothrops atrox is the most important venomous snake in the Brazilian Amazon, causing 80–90 % of the snake envenomings in the region. In the Brazilian Amazon, Bothrops envenoming shows pain, swelling, regional lymphadenopathy, ecchymosis, blistering, and necrosis as the most common local clinical manifestations. Secondary bacterial infections were observed in around 40 % of the Bothrops snakebites. Spontaneous systemic bleeding and acute renal failure are common systemic complications after Bothrops envenomings. It is difficult for riverine and indigenous populations to reach health centers for treatment of snakebites. As a result, the number of cases detected officially is probably underestimated. Current antivenoms (AVs) require conservation in adequate facilities, which are not always available in remote settings. In addition, training of multidisciplinary teams is not always appropriate for indigenous health services regarding AV administration, side effect management, and case monitoring and surveillance. Although clinical research related to venomous animal injuries has increased, most publications are based on case reports and lack methodological rigor. Moreover, outcome definitions, such as severity ranking criteria, were empirically established, making the results even less generalizable. Clinical research from hospital-based studies and community observational studies are needed. In addition to all the above recommendations, the importance of international cooperative efforts toward the control of these neglected health problems through international partnerships, namely, with other Amazonian countries, is highlighted.
Sâmella Silva de Oliveira; Vanderson De Souza Sampaio; Jacqueline De Almeida Gonçalves Sachett; Eliane Campos Alves; Vanessa Costa da Silva; João Arthur Alcântara de Lima; Iran Mendonça da Silva; Luiz Carlos De Lima Ferreira; Hui Wen Fan; Marcus Vinicius Guimarães de Lacerda; Wuelton Marcelo Monteiro. Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives. Toxins and Drug Discovery 2016, 1 -22.
AMA StyleSâmella Silva de Oliveira, Vanderson De Souza Sampaio, Jacqueline De Almeida Gonçalves Sachett, Eliane Campos Alves, Vanessa Costa da Silva, João Arthur Alcântara de Lima, Iran Mendonça da Silva, Luiz Carlos De Lima Ferreira, Hui Wen Fan, Marcus Vinicius Guimarães de Lacerda, Wuelton Marcelo Monteiro. Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives. Toxins and Drug Discovery. 2016; ():1-22.
Chicago/Turabian StyleSâmella Silva de Oliveira; Vanderson De Souza Sampaio; Jacqueline De Almeida Gonçalves Sachett; Eliane Campos Alves; Vanessa Costa da Silva; João Arthur Alcântara de Lima; Iran Mendonça da Silva; Luiz Carlos De Lima Ferreira; Hui Wen Fan; Marcus Vinicius Guimarães de Lacerda; Wuelton Marcelo Monteiro. 2016. "Snakebites in the Brazilian Amazon: Current Knowledge and Perspectives." Toxins and Drug Discovery , no. : 1-22.
Luana V. Senise; Sâmella S. Oliveira; Marcio Y. Yano; Sávio S. Sant'anna; Marcelo L. Santoro; Ida S. Sano-Martins. 79. Hemostatic Disturbances Evoked by Young and Adult Bothrops jararaca Snake Venoms: Analysis of the Envenoming Process and the Recovery after Specific Antivenin Treatment. Toxicon 2012, 60, 134 -135.
AMA StyleLuana V. Senise, Sâmella S. Oliveira, Marcio Y. Yano, Sávio S. Sant'anna, Marcelo L. Santoro, Ida S. Sano-Martins. 79. Hemostatic Disturbances Evoked by Young and Adult Bothrops jararaca Snake Venoms: Analysis of the Envenoming Process and the Recovery after Specific Antivenin Treatment. Toxicon. 2012; 60 (2):134-135.
Chicago/Turabian StyleLuana V. Senise; Sâmella S. Oliveira; Marcio Y. Yano; Sávio S. Sant'anna; Marcelo L. Santoro; Ida S. Sano-Martins. 2012. "79. Hemostatic Disturbances Evoked by Young and Adult Bothrops jararaca Snake Venoms: Analysis of the Envenoming Process and the Recovery after Specific Antivenin Treatment." Toxicon 60, no. 2: 134-135.