This page has only limited features, please log in for full access.

Unclaimed
A F Perna
First Division of Nephrology, Department of Medical Translational Sciences, University of Campania Luigi Vanvitelli, 80131 Naples, Italy

Basic Info

Basic Info is private.

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 26 June 2021 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Vascular calcification (VC) is a risk factor for cardiovascular events and mortality in chronic kidney disease (CKD). Several components influence the occurrence of VC, among which inflammation. A novel uremic toxin, lanthionine, was shown to increase intracellular calcium in endothelial cells and may have a role in VC. A group of CKD patients was selected and divided into patients with a glomerular filtration rate (GFR) of <45 mL/min/1.73 m2 and ≥45 mL/min/1.73 m2. Total Calcium Score (TCS), based on the Agatston score, was assessed as circulating lanthionine and a panel of different cytokines. A hemodialysis patient group was also considered. Lanthionine was elevated in CKD patients, and levels increased significantly in hemodialysis patients with respect to the two CKD groups; in addition, lanthionine increased along with the increase in TCS, starting from one up to three. Interleukin IL-6, IL-8, and Eotaxin were significantly increased in patients with GFR < 45 mL/min/1.73 m2 with respect to those with GFR ≥ 45 mL/min/1.73 m2. IL-1b, IL-7, IL-8, IL-12, Eotaxin, and VEGF increased in calcified patients with respect to the non-calcified. IL-8 and Eotaxin were elevated both in the low GFR group and in the calcified group. We propose that lanthionine, but also IL-8 and Eotaxin, in particular, are a key feature of VC of CKD, with possible marker significance.

ACS Style

Alessandra Perna; Luigi Russo; Vittoria D’Esposito; Pietro Formisano; Dario Bruzzese; Carmela Vigorito; Annapaola Coppola; Patrizia Lombari; Domenico Russo; Diego Ingrosso. Lanthionine, a Novel Uremic Toxin, in the Vascular Calcification of Chronic Kidney Disease: The Role of Proinflammatory Cytokines. International Journal of Molecular Sciences 2021, 22, 6875 .

AMA Style

Alessandra Perna, Luigi Russo, Vittoria D’Esposito, Pietro Formisano, Dario Bruzzese, Carmela Vigorito, Annapaola Coppola, Patrizia Lombari, Domenico Russo, Diego Ingrosso. Lanthionine, a Novel Uremic Toxin, in the Vascular Calcification of Chronic Kidney Disease: The Role of Proinflammatory Cytokines. International Journal of Molecular Sciences. 2021; 22 (13):6875.

Chicago/Turabian Style

Alessandra Perna; Luigi Russo; Vittoria D’Esposito; Pietro Formisano; Dario Bruzzese; Carmela Vigorito; Annapaola Coppola; Patrizia Lombari; Domenico Russo; Diego Ingrosso. 2021. "Lanthionine, a Novel Uremic Toxin, in the Vascular Calcification of Chronic Kidney Disease: The Role of Proinflammatory Cytokines." International Journal of Molecular Sciences 22, no. 13: 6875.

Review
Published: 16 July 2020 in Genes
Reads 0
Downloads 0

Renal disease is the common denominator of a number of underlying disease conditions, whose prevalence has been dramatically increasing over the last two decades. Two aspects are particularly relevant to the subject of this review: (I) most cases are gathered under the umbrella of chronic kidney disease since they require—predictably for several lustrums—continuous clinical monitoring and treatment to slow down disease progression and prevent complications; (II) cardiovascular disease is a terrible burden in this population of patients, in that it claims many lives yearly, while only a scant minority reach the renal disease end stage. Why indeed a review on DNA methylation and renal disease? As we hope to convince you, the present evidence supports the role of the existence of various derangements of the epigenetic control of gene expression in renal disease, which hold the potential to improve our ability, in the future, to more effectively act toward disease progression, predict outcomes and offer novel therapeutic approaches.

ACS Style

Diego Ingrosso; Alessandra F. Perna. DNA Methylation Dysfunction in Chronic Kidney Disease. Genes 2020, 11, 811 .

AMA Style

Diego Ingrosso, Alessandra F. Perna. DNA Methylation Dysfunction in Chronic Kidney Disease. Genes. 2020; 11 (7):811.

Chicago/Turabian Style

Diego Ingrosso; Alessandra F. Perna. 2020. "DNA Methylation Dysfunction in Chronic Kidney Disease." Genes 11, no. 7: 811.

Editorial
Published: 25 May 2020 in Kidney and Blood Pressure Research
Reads 0
Downloads 0
ACS Style

Alessandra F. Perna; Giovanna Capolongo; Francesco Trepiccione; Mariadelina Simeoni; Miriam Zacchia; Diego Ingrosso. COVID-19, Low-Molecular-Weight Heparin, and Hemodialysis. Kidney and Blood Pressure Research 2020, 45, 357 -362.

AMA Style

Alessandra F. Perna, Giovanna Capolongo, Francesco Trepiccione, Mariadelina Simeoni, Miriam Zacchia, Diego Ingrosso. COVID-19, Low-Molecular-Weight Heparin, and Hemodialysis. Kidney and Blood Pressure Research. 2020; 45 (3):357-362.

Chicago/Turabian Style

Alessandra F. Perna; Giovanna Capolongo; Francesco Trepiccione; Mariadelina Simeoni; Miriam Zacchia; Diego Ingrosso. 2020. "COVID-19, Low-Molecular-Weight Heparin, and Hemodialysis." Kidney and Blood Pressure Research 45, no. 3: 357-362.

Review
Published: 11 April 2020 in Toxins
Reads 0
Downloads 0

Several of the uremic toxins, which are difficult to remove by dialysis, originate from the gut bacterial metabolism. This opens opportunities for novel targets trying to decrease circulating levels of these toxins and their pathophysiological effects. The current review focuses on immunomodulatory effects of these toxins both at their side of origin and in the circulation. In the gut end products of the bacterial metabolism such as p-cresol, trimethylamine and H2S affect the intestinal barrier structure and function while in the circulation the related uremic toxins stimulate cells of the immune system. Both conditions contribute to the pro-inflammatory status of patients with chronic kidney disease (CKD). Generation and/or absorption of these toxin precursors could be targeted to decrease plasma levels of their respective uremic toxins and to reduce micro-inflammation in CKD.

ACS Style

Griet Glorieux; Tessa Gryp; Alessandra Perna. Gut-Derived Metabolites and Their Role in Immune Dysfunction in Chronic Kidney Disease. Toxins 2020, 12, 245 .

AMA Style

Griet Glorieux, Tessa Gryp, Alessandra Perna. Gut-Derived Metabolites and Their Role in Immune Dysfunction in Chronic Kidney Disease. Toxins. 2020; 12 (4):245.

Chicago/Turabian Style

Griet Glorieux; Tessa Gryp; Alessandra Perna. 2020. "Gut-Derived Metabolites and Their Role in Immune Dysfunction in Chronic Kidney Disease." Toxins 12, no. 4: 245.

Review
Published: 27 February 2020 in Journal of Clinical Medicine
Reads 0
Downloads 0

Secondary hyperparathyroidism (SHPTH) is a major complication in patients on maintenance hemodialysis burdened with high cardiovascular risk. Hypertension is also a high prevalence complication contributing to an increase in the mortality rate in hemodialysis patients. A possible association between SHPTH and hypertension has been widely reported in the literature and several pathogenetic mechanisms have been described. There is evidence that the decrease of plasma iPTH levels are correlated with hypertension correction in hemodialysis patients undergoing parathyroidectomy and oral calcimimetics administration. We have observed a similar behaviour also in a patient on chronic hemodialysis treated with Etelcalcetide. Even if this is an isolated observation, it could stimulate future investigation, possibly in dedicated clinical trials.

ACS Style

Mariadelina Simeoni; Alessandra F. Perna; Giorgio Fuiano. Secondary Hyperparathyroidism and Hypertension: An Intriguing Couple. Journal of Clinical Medicine 2020, 9, 629 .

AMA Style

Mariadelina Simeoni, Alessandra F. Perna, Giorgio Fuiano. Secondary Hyperparathyroidism and Hypertension: An Intriguing Couple. Journal of Clinical Medicine. 2020; 9 (3):629.

Chicago/Turabian Style

Mariadelina Simeoni; Alessandra F. Perna; Giorgio Fuiano. 2020. "Secondary Hyperparathyroidism and Hypertension: An Intriguing Couple." Journal of Clinical Medicine 9, no. 3: 629.

Editorial
Published: 21 June 2019 in Journal of Nephrology
Reads 0
Downloads 0
ACS Style

Alessandra F. Perna; Diego Ingrosso. Homocysteine and chronic kidney disease: an ongoing narrative. Journal of Nephrology 2019, 32, 673 -675.

AMA Style

Alessandra F. Perna, Diego Ingrosso. Homocysteine and chronic kidney disease: an ongoing narrative. Journal of Nephrology. 2019; 32 (5):673-675.

Chicago/Turabian Style

Alessandra F. Perna; Diego Ingrosso. 2019. "Homocysteine and chronic kidney disease: an ongoing narrative." Journal of Nephrology 32, no. 5: 673-675.

Protocol
Published: 31 May 2019 in Breast Cancer
Reads 0
Downloads 0

In the context of the vascular effects of hydrogen sulfide (H2S), it is known that this gaseous endogenous biological modulator of inflammation, oxidative stress, etc. is a potent vasodilator. Chronic renal failure, a common disease affecting the aging population, is characterized by low levels of H2S in plasma and tissues, which could mediate their typical hypertensive pattern, along with other abnormalities. Lanthionine and homolanthionine, natural non-proteinogenic amino acids, are formed as side products of H2S production. Also in consideration of the intrinsic difficulties in H2S measuring, these compounds have been proposed as reliable and stable markers of H2S synthesis. However, in the setting of chronic renal failure patients on hemodialysis, they represent typical retention products (without ruling out the possibility of an increased intestinal synthesis) and prospective novel uremic toxins. Here, a method utilizing liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) in multiple reaction monitoring ion mode has been developed and evaluated for the determination of these key H2S metabolites in plasma, by using a triple quadrupole mass spectrometer.

ACS Style

Alessandra F. Perna; Francesca Pane; Nunzio Sepe; Carolina Fontanarosa; Gabriella Pinto; Miriam Zacchia; Francesco Trepiccione; Evgeniya Anishchenko; Diego Ingrosso; Piero Pucci; Angela Amoresano. Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry. Breast Cancer 2019, 2007, 9 -17.

AMA Style

Alessandra F. Perna, Francesca Pane, Nunzio Sepe, Carolina Fontanarosa, Gabriella Pinto, Miriam Zacchia, Francesco Trepiccione, Evgeniya Anishchenko, Diego Ingrosso, Piero Pucci, Angela Amoresano. Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry. Breast Cancer. 2019; 2007 ():9-17.

Chicago/Turabian Style

Alessandra F. Perna; Francesca Pane; Nunzio Sepe; Carolina Fontanarosa; Gabriella Pinto; Miriam Zacchia; Francesco Trepiccione; Evgeniya Anishchenko; Diego Ingrosso; Piero Pucci; Angela Amoresano. 2019. "Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry." Breast Cancer 2007, no. : 9-17.

Journal article
Published: 08 May 2019 in International Journal of Molecular Sciences
Reads 0
Downloads 0

(1) The beneficial effects of hydrogen sulfide (H2S) on the cardiovascular and nervous system have recently been re-evaluated. It has been shown that lanthionine, a side product of H2S biosynthesis, previously used as a marker for H2S production, is dramatically increased in circulation in uremia, while H2S release is impaired. Thus, lanthionine could be classified as a novel uremic toxin. Our research was aimed at defining the mechanism(s) for lanthionine toxicity. (2) The effect of lanthionine on H2S release was tested by a novel lead acetate strip test (LAST) in EA.hy926 cell cultures. Effects of glutathione, as a redox agent, were assayed. Levels of sulfane sulfur were evaluated using the SSP4 probe and flow cytometry. Protein content and glutathionylation were analyzed by Western Blotting and immunoprecipitation, respectively. Gene expression and miRNA levels were assessed by qPCR. (3) We demonstrated that, in endothelial cells, lanthionine hampers H2S release; reduces protein content and glutathionylation of transsulfuration enzyme cystathionine-β-synthase; modifies the expression of miR-200c and miR-423; lowers expression of vascular endothelial growth factor VEGF; increases Ca2+ levels. (4) Lanthionine-induced alterations in cell cultures, which involve both sulfur amino acid metabolism and calcium homeostasis, are consistent with uremic dysfunctional characteristics and further support the uremic toxin role of this amino acid.

ACS Style

Carmela Vigorito; Evgeniya Anishchenko; Luigi Mele; Giovanna Capolongo; Francesco Trepiccione; Miriam Zacchia; Patrizia Lombari; Rosanna Capasso; Diego Ingrosso; Alessandra F. Perna. Uremic Toxin Lanthionine Interferes with the Transsulfuration Pathway, Angiogenetic Signaling and Increases Intracellular Calcium. International Journal of Molecular Sciences 2019, 20, 2269 .

AMA Style

Carmela Vigorito, Evgeniya Anishchenko, Luigi Mele, Giovanna Capolongo, Francesco Trepiccione, Miriam Zacchia, Patrizia Lombari, Rosanna Capasso, Diego Ingrosso, Alessandra F. Perna. Uremic Toxin Lanthionine Interferes with the Transsulfuration Pathway, Angiogenetic Signaling and Increases Intracellular Calcium. International Journal of Molecular Sciences. 2019; 20 (9):2269.

Chicago/Turabian Style

Carmela Vigorito; Evgeniya Anishchenko; Luigi Mele; Giovanna Capolongo; Francesco Trepiccione; Miriam Zacchia; Patrizia Lombari; Rosanna Capasso; Diego Ingrosso; Alessandra F. Perna. 2019. "Uremic Toxin Lanthionine Interferes with the Transsulfuration Pathway, Angiogenetic Signaling and Increases Intracellular Calcium." International Journal of Molecular Sciences 20, no. 9: 2269.

Protocol
Published: 01 February 2019 in Methods and Protocols
Reads 0
Downloads 0

Hydrogen sulfide (H2S) is the most recently established gaseous vasodilator, enzymatically produced from cysteine metabolism, involved in a number of pathophysiological processes. However, its accurate detection in vivo is critical due to its volatility and tendency to form sulfane sulfur derivatives, thus limiting the data interpretation of its biological roles. We developed new applications of the simple and rapid method to measure H2S release in cell culture systems, based on the lead acetate strip test. This test, previously prevalently used in microbiology, was compared with the agar trap method, applied, in parallel, on both cell cultures and cell-free samples. Sulfane sulfur represents the major species derived from intracellular H2S. Various fluorescent probes are available for quantitation of H2S derivatives intracellularly. We present here an alternative to the classic imaging method for sulfane sulfur evaluation, running on a flow cytometer, based on SSP4 probe labeling. Flow cytometry turned out to be more direct, fully quantitative and less time-consuming compared to microscopy and more precise with respect to the fluorescence multi-plate reader assay. The new application methods for H2S determination appear to be fully suitable for the analysis of H2S release and sulfane sulfur content in biological samples.

ACS Style

Evgeniya Anishchenko; Carmela Vigorito; Luigi Mele; Patrizia Lombari; Alessandra F. Perna; Diego Ingrosso. Novel Applications of Lead Acetate and Flow Cytometry Methods for Detection of Sulfur-Containing Molecules. Methods and Protocols 2019, 2, 13 .

AMA Style

Evgeniya Anishchenko, Carmela Vigorito, Luigi Mele, Patrizia Lombari, Alessandra F. Perna, Diego Ingrosso. Novel Applications of Lead Acetate and Flow Cytometry Methods for Detection of Sulfur-Containing Molecules. Methods and Protocols. 2019; 2 (1):13.

Chicago/Turabian Style

Evgeniya Anishchenko; Carmela Vigorito; Luigi Mele; Patrizia Lombari; Alessandra F. Perna; Diego Ingrosso. 2019. "Novel Applications of Lead Acetate and Flow Cytometry Methods for Detection of Sulfur-Containing Molecules." Methods and Protocols 2, no. 1: 13.

Review
Published: 23 January 2019 in Journal of Nephrology
Reads 0
Downloads 0

The gut microbiota is considered to be a novel important factor to take into account in the pathogenesis of chronic kidney disease and uremia. Much attention has been paid to specific uremic retention solutes of microbial origin, such as indoxyl sulfate, p-cresyl sulfate, and trimethylamine-N-oxide. However, other novel less well studied compounds, such as hydrogen sulfide and related sulfur metabolites (sulfane sulfur, lanthionine, etc.), should be included in a more comprehensive appraisal of this topic, in light of the potential therapeutic opportunities for the future.

ACS Style

Alessandra F. Perna; Griet Glorieux; Miriam Zacchia; Francesco Trepiccione; Giovanna Capolongo; Carmela Vigorito; Evgeniya Anishchenko; Diego Ingrosso. The role of the intestinal microbiota in uremic solute accumulation: a focus on sulfur compounds. Journal of Nephrology 2019, 32, 733 -740.

AMA Style

Alessandra F. Perna, Griet Glorieux, Miriam Zacchia, Francesco Trepiccione, Giovanna Capolongo, Carmela Vigorito, Evgeniya Anishchenko, Diego Ingrosso. The role of the intestinal microbiota in uremic solute accumulation: a focus on sulfur compounds. Journal of Nephrology. 2019; 32 (5):733-740.

Chicago/Turabian Style

Alessandra F. Perna; Griet Glorieux; Miriam Zacchia; Francesco Trepiccione; Giovanna Capolongo; Carmela Vigorito; Evgeniya Anishchenko; Diego Ingrosso. 2019. "The role of the intestinal microbiota in uremic solute accumulation: a focus on sulfur compounds." Journal of Nephrology 32, no. 5: 733-740.

Journal article
Published: 29 April 2018 in International Journal of Molecular Sciences
Reads 0
Downloads 0

The non-proteinogenic amino acid lanthionine is a byproduct of hydrogen sulfide biosynthesis: the third endogenous vasodilator gas, after nitric oxide and carbon monoxide. While hydrogen sulfide is decreased in uremic patients on hemodialysis, lanthionine is increased and has been proposed as a new uremic toxin, since it is able to impair hydrogen sulfide production in hepatoma cells. To characterize lanthionine as a uremic toxin, we explored its effects during the early development of the zebrafish (Danio rerio), a widely used model to study the organ and tissue alterations induced by xenobiotics. Lanthionine was employed at concentrations reproducing those previously detected in uremia. Light-induced visual motor response was also studied by means of the DanioVision system. Treatment of zebrafish embryos with lanthionine determined acute phenotypical alterations, on heart organogenesis (disproportion in cardiac chambers), increased heart beating, and arrhythmia. Lanthionine also induced locomotor alterations in zebrafish embryos. Some of these effects could be counteracted by glutathione. Lanthionine exerted acute effects on transsulfuration enzymes and the expression of genes involved in inflammation and metabolic regulation, and modified microRNA expression in a way comparable with some alterations detected in uremia. Lanthionine meets the criteria for classification as a uremic toxin. Zebrafish can be successfully used to explore uremic toxin effects.

ACS Style

Alessandra F. Perna; Evgeniya Anishchenko; Carmela Vigorito; Miriam Zacchia; Francesco Trepiccione; Salvatore D’Aniello; Diego Ingrosso. Zebrafish, a Novel Model System to Study Uremic Toxins: The Case for the Sulfur Amino Acid Lanthionine. International Journal of Molecular Sciences 2018, 19, 1323 .

AMA Style

Alessandra F. Perna, Evgeniya Anishchenko, Carmela Vigorito, Miriam Zacchia, Francesco Trepiccione, Salvatore D’Aniello, Diego Ingrosso. Zebrafish, a Novel Model System to Study Uremic Toxins: The Case for the Sulfur Amino Acid Lanthionine. International Journal of Molecular Sciences. 2018; 19 (5):1323.

Chicago/Turabian Style

Alessandra F. Perna; Evgeniya Anishchenko; Carmela Vigorito; Miriam Zacchia; Francesco Trepiccione; Salvatore D’Aniello; Diego Ingrosso. 2018. "Zebrafish, a Novel Model System to Study Uremic Toxins: The Case for the Sulfur Amino Acid Lanthionine." International Journal of Molecular Sciences 19, no. 5: 1323.

Journal article
Published: 17 January 2018 in Pharmaceutics
Reads 0
Downloads 0

Chitosan is biopolymer with promising properties in wound healing. Chronic wounds represent a significant burden to both the patient and the medical system. Among chronic wounds, pressure ulcers are one of the most common types of complex wound. The efficacy and the tolerability of chitosan gel formulation, prepared into the hospital pharmacy, in the treatment of pressure ulcers of moderate severity were evaluated. The endpoint of this phase II study was the reduction of the area of the lesion by at least 20% after four weeks of treatment. Thus, 20 adult volunteers with pressure ulcers within predetermined parameters were involved in a 30 days study. Dressing change was performed twice a week at outpatient clinic upon chronic wounds management. In the 90% of patients involved in the study, the treatment was effective, with a reduction of the area of the lesion and wound healing progress. The study demonstrated the efficacy of the gel formulation for treatment of pressure ulcers, also providing a strong reduction of patient management costs.

ACS Style

Virginia Campani; Eliana Pagnozzi; Ilaria Mataro; Laura Mayol; Alessandra Perna; Floriana D’Urso; Antonietta Carillo; Maria Cammarota; Maria Chiara Maiuri; Giuseppe De Rosa. Chitosan Gel to Treat Pressure Ulcers: A Clinical Pilot Study. Pharmaceutics 2018, 10, 15 .

AMA Style

Virginia Campani, Eliana Pagnozzi, Ilaria Mataro, Laura Mayol, Alessandra Perna, Floriana D’Urso, Antonietta Carillo, Maria Cammarota, Maria Chiara Maiuri, Giuseppe De Rosa. Chitosan Gel to Treat Pressure Ulcers: A Clinical Pilot Study. Pharmaceutics. 2018; 10 (1):15.

Chicago/Turabian Style

Virginia Campani; Eliana Pagnozzi; Ilaria Mataro; Laura Mayol; Alessandra Perna; Floriana D’Urso; Antonietta Carillo; Maria Cammarota; Maria Chiara Maiuri; Giuseppe De Rosa. 2018. "Chitosan Gel to Treat Pressure Ulcers: A Clinical Pilot Study." Pharmaceutics 10, no. 1: 15.

Journal article
Published: 01 November 2017 in Journal of Renal Nutrition
Reads 0
Downloads 0

The triad composed by α-Klotho, fibroblast growth factor-23, and its receptor are involved in the pathogenesis of chronic kidney disease-mineral and bone disorder. A disintegrin and metalloproteinase 17 (ADAM17) is a metalloproteinase causing the proteolytic shedding of α-Klotho from the cell membrane, and its role in chronic kidney disease-mineral and bone disorder is not yet known. We studied the circulating levels of the above-mentioned mediators in patients with secondary hyperparathyroidism due to uremia, compared to control subjects, as well as in patients with primary hyperparathyroidism. We also measured the immunofluorescence pattern of the relevant tissue proteins in specimens obtained from patients undergoing parathyroid surgery for secondary compared to primary hyperparathyroidism. Results showed that α-Klotho tissue levels are reduced, in the presence of increased ADAM17 tissue levels. In addition, we showed increased serum levels of the main product of ADAM17 proteolytic activity, tumor necrosis factor-α. Thus, we found a paradoxical situation, in secondary compared to primary hyperparathyroidism, that is, that in the face of increased tumor necrosis factor-α in circulation, both soluble and tissue α-Klotho are reduced significantly, despite increased tissue ADAM17. In conclusion, tissue and serum levels of α-Klotho seem to have become independent from the regulation induced by ADAM17, which constitutes therefore another tassel in the impaired α-Klotho-FGF23 receptor axis present in uremia.

ACS Style

Alessandra F. Perna; Alessandra Pizza; Annarita Di Nunzio; Rocco Bellantone; Marco Raffaelli; Tommaso Cicchella; Giovanni Conzo; Luigi Santini; Miriam Zacchia; Francesco Trepiccione; Diego Ingrosso. ADAM17, a New Player in the Pathogenesis of Chronic Kidney Disease–Mineral and Bone Disorder. Journal of Renal Nutrition 2017, 27, 453 -457.

AMA Style

Alessandra F. Perna, Alessandra Pizza, Annarita Di Nunzio, Rocco Bellantone, Marco Raffaelli, Tommaso Cicchella, Giovanni Conzo, Luigi Santini, Miriam Zacchia, Francesco Trepiccione, Diego Ingrosso. ADAM17, a New Player in the Pathogenesis of Chronic Kidney Disease–Mineral and Bone Disorder. Journal of Renal Nutrition. 2017; 27 (6):453-457.

Chicago/Turabian Style

Alessandra F. Perna; Alessandra Pizza; Annarita Di Nunzio; Rocco Bellantone; Marco Raffaelli; Tommaso Cicchella; Giovanni Conzo; Luigi Santini; Miriam Zacchia; Francesco Trepiccione; Diego Ingrosso. 2017. "ADAM17, a New Player in the Pathogenesis of Chronic Kidney Disease–Mineral and Bone Disorder." Journal of Renal Nutrition 27, no. 6: 453-457.

Review
Published: 10 January 2017 in Toxins
Reads 0
Downloads 0

Lanthionine is a nonproteinogenic amino acid, composed of two alanine residues that are crosslinked on their β-carbon atoms by a thioether linkage. It is biosynthesized from the condensation of two cysteine molecules, while the related compound homolanthionine is formed from the condensation of two homocysteine molecules. The reactions can be carried out by either cystathionine-β-synthase (CBS) or cystathionine-γ-lyase (CSE) independently, in the alternate reactions of the transsulfuration pathway devoted to hydrogen sulfide biosynthesis. Low plasma total hydrogen sulfide levels, probably due to reduced CSE expression, are present in uremia, while homolanthionine and lanthionine accumulate in blood, the latter several fold. Uremic patients display a derangement of sulfur amino acid metabolism with a high prevalence of hyperhomocysteinemia. Uremia is associated with a high cardiovascular mortality, the causes of which are still not completely explained, but are related to uremic toxicity, due to the accumulation of retention products. Lanthionine inhibits hydrogen sulfide production in hepatoma cells, possibly through CBS inhibition, thus providing some basis for the biochemical mechanism, which may significantly contribute to alterations of metabolism sulfur compounds in these subjects (e.g., high homocysteine and low hydrogen sulfide). We therefore suggest that lanthionine is a novel uremic toxin.

ACS Style

Alessandra F. Perna; Miriam Zacchia; Francesco Trepiccione; Diego Ingrosso. The Sulfur Metabolite Lanthionine: Evidence for a Role as a Novel Uremic Toxin. Toxins 2017, 9, 26 .

AMA Style

Alessandra F. Perna, Miriam Zacchia, Francesco Trepiccione, Diego Ingrosso. The Sulfur Metabolite Lanthionine: Evidence for a Role as a Novel Uremic Toxin. Toxins. 2017; 9 (1):26.

Chicago/Turabian Style

Alessandra F. Perna; Miriam Zacchia; Francesco Trepiccione; Diego Ingrosso. 2017. "The Sulfur Metabolite Lanthionine: Evidence for a Role as a Novel Uremic Toxin." Toxins 9, no. 1: 26.

Clinical trial
Published: 01 July 2016 in Biochimie
Reads 0
Downloads 0

Dialysis patients display a high cardiovascular mortality, the causes of which are still not completely explained, but are related to uremic toxicity. Among uremic toxins, homocysteine and cysteine are both substrates of cystathionine β-synthase and cystathionine γ-lyase in hydrogen sulfide biosynthesis, leading to the formation of two sulfur metabolites, lanthionine and homolanthionine, considered stable indirect biomarkers of its production. Hydrogen sulfide is involved in the modulation of multiple pathophysiological responses. In uremia, we have demonstrated low plasma total hydrogen sulfide levels, due to reduced cystathionine γ-lyase expression. Plasma hydrogen sulfide levels were measured in hemodialysis patients and healthy controls with three different techniques in comparison, allowing to discern the different pools of this gas. The protein-bound (the one thought to be the most active) and acid-labile forms are significantly decreased, while homolanthionine, but especially lanthionine, accumulate in the blood of uremic patients. The hemodialysis regimen plays a role in determining sulfur compounds levels, and lanthionine is partially removed by a single dialysis session. Lanthionine inhibits hydrogen sulfide production in cell cultures under conditions comparable to in vivo ones. We therefore propose that lanthionine is a novel uremic toxin. The possible role of high lanthionine as a contributor to the genesis of hyperhomocysteinemia in uremia is discussed.

ACS Style

Alessandra F. Perna; Annarita Di Nunzio; Angela Amoresano; Francesca Pane; Carolina Fontanarosa; Piero Pucci; Carmela Vigorito; Giovanni Cirillo; Miriam Zacchia; Francesco Trepiccione; Diego Ingrosso. Divergent behavior of hydrogen sulfide pools and of the sulfur metabolite lanthionine, a novel uremic toxin, in dialysis patients. Biochimie 2016, 126, 97 -107.

AMA Style

Alessandra F. Perna, Annarita Di Nunzio, Angela Amoresano, Francesca Pane, Carolina Fontanarosa, Piero Pucci, Carmela Vigorito, Giovanni Cirillo, Miriam Zacchia, Francesco Trepiccione, Diego Ingrosso. Divergent behavior of hydrogen sulfide pools and of the sulfur metabolite lanthionine, a novel uremic toxin, in dialysis patients. Biochimie. 2016; 126 ():97-107.

Chicago/Turabian Style

Alessandra F. Perna; Annarita Di Nunzio; Angela Amoresano; Francesca Pane; Carolina Fontanarosa; Piero Pucci; Carmela Vigorito; Giovanni Cirillo; Miriam Zacchia; Francesco Trepiccione; Diego Ingrosso. 2016. "Divergent behavior of hydrogen sulfide pools and of the sulfur metabolite lanthionine, a novel uremic toxin, in dialysis patients." Biochimie 126, no. : 97-107.

Comment
Published: 19 December 2015 in Nephrology Dialysis Transplantation
Reads 0
Downloads 0

Chronic kidney disease (CKD) is characterized by the progressive loss of renal function, leading to the gradual retention of compounds normally excreted or actively metabolized by the body, termed uremic toxins as a whole [1], among which the protein-bound moieties, such as indoxyl sulfate, asymmetric dimethylarginine, p-cresol and homocysteine, are much investigated at present.

ACS Style

Alessandra F. Perna; Diego Ingrosso. Atherosclerosis determinants in renal disease: how much is homocysteine involved? Nephrology Dialysis Transplantation 2015, 31, 860 -863.

AMA Style

Alessandra F. Perna, Diego Ingrosso. Atherosclerosis determinants in renal disease: how much is homocysteine involved? Nephrology Dialysis Transplantation. 2015; 31 (6):860-863.

Chicago/Turabian Style

Alessandra F. Perna; Diego Ingrosso. 2015. "Atherosclerosis determinants in renal disease: how much is homocysteine involved?" Nephrology Dialysis Transplantation 31, no. 6: 860-863.

Comparative study
Published: 20 August 2015 in Journal of Human Hypertension
Reads 0
Downloads 0

Late arterial hypertension has been identified as a major predictor for morbidity and mortality in aortic coarctation (AoC) patients. Few data are available about efficacy and tolerability of angiotensin converting enzyme inhibitors vs beta-blockers in young AoC patients. This study aimed to evaluate the tolerability and efficacy on 24-h blood pressure (BP) and left ventricular mass/height2.7 (LVMI), of atenolol vs enalapril. We enrolled consecutive AoC hypertensive patients with (a) no history of BP treatment or after >48 h of withdrawn, (b) aged 6–20 years, (c) body mass index (BMI) 12 months from a successful AoC repair and (e) no major associated cardiovascular abnormalities. All patient were evaluated with 24-h ambulatory BP monitoring, standard echocardiography, strain–strain rate imaging, at enrolment, 3, 6 and 12 months of treatment. We studied 51 AoC patients (13±3.9 years, BMI: 21.4±4.3 kg m–2). Patients were randomly assigned at atenolol treatment (n=26), or enalapril treatment (n=25). The mean follow-up duration was 11±2 months. Both drugs were able to significantly reduce 24-systolic BP (SBP; atenolol: 133±11 mm Hg vs 124±16 mm Hg, P=0.016; enalapril: 135±6 mm Hg vs 127±7 mm Hg, P=0.001). Only enalapril was able to significantly reduce LVMI (47±12 vs 39.6±10 g m–2.7, P=0.016). Only in atenolol group in two cases (7.7%) drug withdrawal was needed because of adverse events. Enalapril and atenolol are similarly effective in reducing SBP. However, only enalapril demonstrated a significant reduction of LVMI. In no case, enalapril was stopped because of adverse events.

ACS Style

Giovanni Di Salvo; B Castaldi; S Gala; L Baldini; F Del Gaizo; F A D'aiello; A Mormile; A Rea; G Scognamiglio; Giuseppe Pacileo; S Keating; B M Fadel; L Berrino; Alessandra Perna; M G Russo; R Calabrò. Atenolol vs enalapril in young hypertensive patients after successful repair of aortic coarctation. Journal of Human Hypertension 2015, 30, 363 -367.

AMA Style

Giovanni Di Salvo, B Castaldi, S Gala, L Baldini, F Del Gaizo, F A D'aiello, A Mormile, A Rea, G Scognamiglio, Giuseppe Pacileo, S Keating, B M Fadel, L Berrino, Alessandra Perna, M G Russo, R Calabrò. Atenolol vs enalapril in young hypertensive patients after successful repair of aortic coarctation. Journal of Human Hypertension. 2015; 30 (6):363-367.

Chicago/Turabian Style

Giovanni Di Salvo; B Castaldi; S Gala; L Baldini; F Del Gaizo; F A D'aiello; A Mormile; A Rea; G Scognamiglio; Giuseppe Pacileo; S Keating; B M Fadel; L Berrino; Alessandra Perna; M G Russo; R Calabrò. 2015. "Atenolol vs enalapril in young hypertensive patients after successful repair of aortic coarctation." Journal of Human Hypertension 30, no. 6: 363-367.

Research article
Published: 11 August 2015 in PLOS ONE
Reads 0
Downloads 0

Pleural malignant mesothelioma (MPM) is a detrimental neoplasm affecting pleural sheets and determining a high rate of mortality. In this study, we have enrolled 14 consecutive patients (13 males and 1 female) with MPM (mean age: 70.3 ± 4.6 years). We have collected serum for the determination of a miRNA profiling using a low-density microarray real time PCR system in the serum of patients and comparing it with that one of 10 control counterparts affected by not-cancer-related pleural effusions. In the patients 5 miRNAs were up-regulated (miR101, miR25, miR26b, miR335 and miR433), 2 miRNA were downregulated (miR191, miR223) and two miRNAs were expressed exclusively in patients (miR29a and miR516). Based upon the changes in the expression of the above mentioned miRNAs we detected two distinctive miRNA signatures predicting histotype and survival in these patients: I) patients with more than 3/9 upregulated miRNAs or 3/9 upregulated miRNAs and miR516 not recordable or unchanged (signature A); II) patients with at least 3/9 downregulated or unchanged miRNAs and/or miR29a downregulated (signature B). Based upon these criteria, 5 patients were stratified in signature A and the remaining 9 in signature B. Patients with signature A had a significant shorter median survival than those with signature B (7 months vs. 17 months, 95% CI: 0.098–1.72, p = 0.0021), had a sarcomatoid or mixed histological MPM subtype and were diagnosed in stage II (3/5) and stage III (2/5). In conclusion, we suggest that miRNA signature A is predictive of sarcomatoid histotype and of worse prognosis in MPM.

ACS Style

Monica Lamberti; Rosanna Capasso; Angela Lombardi; Marina Di Domenico; Alfonso Fiorelli; Antonia Feola; Alessandra F. Perna; Mario Santini; Michele Caraglia; Diego Ingrosso. Two Different Serum MiRNA Signatures Correlate with the Clinical Outcome and Histological Subtype in Pleural Malignant Mesothelioma Patients. PLOS ONE 2015, 10, e0135331 .

AMA Style

Monica Lamberti, Rosanna Capasso, Angela Lombardi, Marina Di Domenico, Alfonso Fiorelli, Antonia Feola, Alessandra F. Perna, Mario Santini, Michele Caraglia, Diego Ingrosso. Two Different Serum MiRNA Signatures Correlate with the Clinical Outcome and Histological Subtype in Pleural Malignant Mesothelioma Patients. PLOS ONE. 2015; 10 (8):e0135331.

Chicago/Turabian Style

Monica Lamberti; Rosanna Capasso; Angela Lombardi; Marina Di Domenico; Alfonso Fiorelli; Antonia Feola; Alessandra F. Perna; Mario Santini; Michele Caraglia; Diego Ingrosso. 2015. "Two Different Serum MiRNA Signatures Correlate with the Clinical Outcome and Histological Subtype in Pleural Malignant Mesothelioma Patients." PLOS ONE 10, no. 8: e0135331.

Research article
Published: 30 January 2015 in PLOS ONE
Reads 0
Downloads 0

Human mesenchymal stem cells (hMSCs), the precursors of osteoblasts during osteogenesis, play a role in the balance of bone formation and resorption, but their functioning in uremia has not been well defined. To study the effects of the uremic milieu on osteogenic properties, we applied an in vitro assay culturing hMSCs in osteogenic medium supplemented with serum from healthy donors and from uremic patients on hemodialysis. Compared to control, serum from uremic patients induces, in hMSC cultures, a modification of several key regulators of bone remodeling, in particular a reduction of the ratio Receptor Activator of Nuclear factor Kappa B Receptor (RANKL) over osteoprotegerin, indicating an adaptive response of the system to favor osteogenesis over osteoclastosis. However, the levels of osteopontin, osteocalcin, and collagen type I, are increased in cell medium, while BMP-2, and alizarin red staining were decreased, pointing to a reduction of bone formation favoring resorption. Selected uremic toxins, such as p-cresylsulfate, p-cresylglucuronide, parathyroid hormone, indoxyl sulfate, asymmetric dimethylarginine, homocysteine, were able to mimic some of the effects of whole serum from uremic patients. Serum from cinacalcet-treated patients antagonizes these effects. Hydrogen sulfide (H2S) donors as well as hemodialysis treatment are able to induce beneficial effects. In conclusion, bone modifications in uremia are influenced by the capability of the uremic milieu to alter hMSC osteogenic differentiation. Cinacalcet, H2S donors and a hemodialysis session can ameliorate the hampered calcium deposition.

ACS Style

Diana Lanza; Alessandra F. Perna; Adriana Oliva; Raymond Vanholder; Anneleen Pletinck; Salvatore Guastafierro; Annarita Di Nunzio; Carmela Vigorito; Giovambattista Capasso; Vera Jankowski; Joachim Jankowski; Diego Ingrosso. Impact of the Uremic Milieu on the Osteogenic Potential of Mesenchymal Stem Cells. PLOS ONE 2015, 10, e0116468 .

AMA Style

Diana Lanza, Alessandra F. Perna, Adriana Oliva, Raymond Vanholder, Anneleen Pletinck, Salvatore Guastafierro, Annarita Di Nunzio, Carmela Vigorito, Giovambattista Capasso, Vera Jankowski, Joachim Jankowski, Diego Ingrosso. Impact of the Uremic Milieu on the Osteogenic Potential of Mesenchymal Stem Cells. PLOS ONE. 2015; 10 (1):e0116468.

Chicago/Turabian Style

Diana Lanza; Alessandra F. Perna; Adriana Oliva; Raymond Vanholder; Anneleen Pletinck; Salvatore Guastafierro; Annarita Di Nunzio; Carmela Vigorito; Giovambattista Capasso; Vera Jankowski; Joachim Jankowski; Diego Ingrosso. 2015. "Impact of the Uremic Milieu on the Osteogenic Potential of Mesenchymal Stem Cells." PLOS ONE 10, no. 1: e0116468.

Journal article
Published: 01 January 2015 in Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia
Reads 0
Downloads 0
ACS Style

Francesco Trepiccione; Alessandra F Perna. [Hyperuricemia and cardiovascular diseases: from phylogenesys to patogenetic mechanisms]. Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia 2015, 32, 1 .

AMA Style

Francesco Trepiccione, Alessandra F Perna. [Hyperuricemia and cardiovascular diseases: from phylogenesys to patogenetic mechanisms]. Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia. 2015; 32 ():1.

Chicago/Turabian Style

Francesco Trepiccione; Alessandra F Perna. 2015. "[Hyperuricemia and cardiovascular diseases: from phylogenesys to patogenetic mechanisms]." Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia 32, no. : 1.