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University Educator/Researcher
01 August 2010 - 01 September 2021
PALABRAS CLAVE: Caries; Infiltración de resina; Caries interproximal; Barniz de flúor; Tratamiento mínimamente invasivo; Epistemonikos; GRADE
Ana María Rojas-Gómez; Francisca Verdugo-Paiva; Julián Balanta-Melo. Infiltración de resina y barniz de flúor para el tratamiento de caries interproximales no cavitadas en dentición permanente. International journal of interdisciplinary dentistry 2021, 14, 105 -109.
AMA StyleAna María Rojas-Gómez, Francisca Verdugo-Paiva, Julián Balanta-Melo. Infiltración de resina y barniz de flúor para el tratamiento de caries interproximales no cavitadas en dentición permanente. International journal of interdisciplinary dentistry. 2021; 14 (1):105-109.
Chicago/Turabian StyleAna María Rojas-Gómez; Francisca Verdugo-Paiva; Julián Balanta-Melo. 2021. "Infiltración de resina y barniz de flúor para el tratamiento de caries interproximales no cavitadas en dentición permanente." International journal of interdisciplinary dentistry 14, no. 1: 105-109.
PALABRAS CLAVE: Caries; Infiltración de resina; Caries interproximal; Barniz de flúor; Tratamiento mínimamente invasivo; Epistemonikos; GRADE
Ana María Rojas-Gómez; Francisca Verdugo-Paiva; Julián Balanta-Melo. Infiltración de resina y barniz de flúor para el tratamiento de caries interproximales no cavitadas en dentición temporal. International journal of interdisciplinary dentistry 2021, 14, 100 -104.
AMA StyleAna María Rojas-Gómez, Francisca Verdugo-Paiva, Julián Balanta-Melo. Infiltración de resina y barniz de flúor para el tratamiento de caries interproximales no cavitadas en dentición temporal. International journal of interdisciplinary dentistry. 2021; 14 (1):100-104.
Chicago/Turabian StyleAna María Rojas-Gómez; Francisca Verdugo-Paiva; Julián Balanta-Melo. 2021. "Infiltración de resina y barniz de flúor para el tratamiento de caries interproximales no cavitadas en dentición temporal." International journal of interdisciplinary dentistry 14, no. 1: 100-104.
The masticatory system is a complex and highly organized group of structures, including craniofacial bones (maxillae and mandible), muscles, teeth, joints, and neurovascular elements. While the musculoskeletal structures of the head and neck are known to have a different embryonic origin, morphology, biomechanical demands, and biochemical characteristics than the trunk and limbs, their particular molecular basis and cell biology have been much less explored. In the last decade, the concept of muscle-bone crosstalk has emerged, comprising both the loads generated during muscle contraction and a biochemical component through soluble molecules. Bone cells embedded in the mineralized tissue respond to the biomechanical input by releasing molecular factors that impact the homeostasis of the attaching skeletal muscle. In the same way, muscle-derived factors act as soluble signals that modulate the remodeling process of the underlying bones. This concept of muscle-bone crosstalk at a molecular level is particularly interesting in the mandible, due to its tight anatomical relationship with one of the biggest and strongest masticatory muscles, the masseter. However, despite the close physical and physiological interaction of both tissues for proper functioning, this topic has been poorly addressed. Here we present one of the most detailed reviews of the literature to date regarding the biomechanical and biochemical interaction between muscles and bones of the masticatory system, both during development and in physiological or pathological remodeling processes. Evidence related to how masticatory function shapes the craniofacial bones is discussed, and a proposal presented that the masticatory muscles and craniofacial bones serve as secretory tissues. We furthermore discuss our current findings of myokines-release from masseter muscle in physiological conditions, during functional adaptation or pathology, and their putative role as bone-modulators in the craniofacial system. Finally, we address the physiological implications of the crosstalk between muscles and bones in the masticatory system, analyzing pathologies or clinical procedures in which the alteration of one of them affects the homeostasis of the other. Unveiling the mechanisms of muscle-bone crosstalk in the masticatory system opens broad possibilities for understanding and treating temporomandibular disorders, which severely impair the quality of life, with a high cost for diagnosis and management.
Sonja Buvinic; Julián Balanta-Melo; Kornelius Kupczik; Walter Vásquez; Carolina Beato; Viviana Toro-Ibacache. Muscle-Bone Crosstalk in the Masticatory System: From Biomechanical to Molecular Interactions. Frontiers in Endocrinology 2021, 11, 1 .
AMA StyleSonja Buvinic, Julián Balanta-Melo, Kornelius Kupczik, Walter Vásquez, Carolina Beato, Viviana Toro-Ibacache. Muscle-Bone Crosstalk in the Masticatory System: From Biomechanical to Molecular Interactions. Frontiers in Endocrinology. 2021; 11 ():1.
Chicago/Turabian StyleSonja Buvinic; Julián Balanta-Melo; Kornelius Kupczik; Walter Vásquez; Carolina Beato; Viviana Toro-Ibacache. 2021. "Muscle-Bone Crosstalk in the Masticatory System: From Biomechanical to Molecular Interactions." Frontiers in Endocrinology 11, no. : 1.
The ongoing Coronavirus Disease 2019 (COVID-19) pandemic has triggered the paralysis of dental services ascribed to the potential spread of severe acute respiratory syndrome (SARS)-CoV-2. Aerosol-generating procedures (AGPs) are common in dentistry, which in turn increase the risk of infection of the dental personnel due to the salivary presence of SARS-CoV-2 in COVID-19 patients. The use of rubber dam isolation (RDI) and high-volume evacuators (HVE) during AGPs is recommended to control dental aerosols, but the evidence about their effectiveness is scarce. This first study aimed to compare, in a simulated patient, the effectiveness of the following strategies: standard suction (SS), RDI and RDI + HVE. Using the laser diffraction technique, the effect of each condition on the volume distribution, average size and concentration of coarse (PM10), fine (PM2.5) and ultrafine (PM0.1) particles were evaluated. During the teeth drilling, the highest volume fraction of dental aerosol particles with SS was below 1 μm of aerodynamic diameter. Additionally, the RDI + HVE significantly reduced both the ultrafine dental aerosol particles and the concentration of total particulate matter. AGPs represent a potential risk for airborne infections in dentistry. Taken together, these preliminary results suggest that isolation and high-volume suction are effective to reduce ultrafine dental aerosol particles.
Julián Balanta-Melo; Albio Gutiérrez; Gustavo Sinisterra; María Del Mar Díaz-Posso; David Gallego; Judy Villavicencio; Adolfo Contreras. Rubber Dam Isolation and High-Volume Suction Reduce Ultrafine Dental Aerosol Particles: An Experiment in a Simulated Patient. Applied Sciences 2020, 10, 6345 .
AMA StyleJulián Balanta-Melo, Albio Gutiérrez, Gustavo Sinisterra, María Del Mar Díaz-Posso, David Gallego, Judy Villavicencio, Adolfo Contreras. Rubber Dam Isolation and High-Volume Suction Reduce Ultrafine Dental Aerosol Particles: An Experiment in a Simulated Patient. Applied Sciences. 2020; 10 (18):6345.
Chicago/Turabian StyleJulián Balanta-Melo; Albio Gutiérrez; Gustavo Sinisterra; María Del Mar Díaz-Posso; David Gallego; Judy Villavicencio; Adolfo Contreras. 2020. "Rubber Dam Isolation and High-Volume Suction Reduce Ultrafine Dental Aerosol Particles: An Experiment in a Simulated Patient." Applied Sciences 10, no. 18: 6345.
Non-traditional bisphosphonates, that is, bisphosphonates that do not inhibit osteoclast viability or function, were initially reported in the 1990s by Socrates Papapoulos' group. Originally designed to study the role of the R1 residue of aminobisphosphonates on bisphosphonate affinity for hydroxyapatite, these modified bisphosphonates retained similar affinity for mineralized bone as their parent compounds, but they lacked the potential to inhibit the mevalonate pathway or bone resorption. We found that, similar to classical bisphosphonates, these non-traditional compounds prevented osteoblast and osteocyte apoptosis in vitro through a pathway that requires the expression of the gap junction protein connexin 43, and the activation of the Src/MEK/ERK signaling pathway. Furthermore, one of those compounds named IG9402 (also known as amino-olpadronate or lidadronate), was able to inhibit osteoblast and osteocyte apoptosis, without affecting osteoclast number or bone resorption in vivo in a model of glucocorticoid-induced osteoporosis. IG9402 administration also ameliorated the decrease in bone mass and in bone mechanical properties induced by glucocorticoids. Similarly, IG9402 prevented apoptosis of osteoblastic cells in a model of immobilization due to hindlimb unloading. However, in this case, the bisphosphonate was not able to preserve the bone mass, and only partially prevented the decrease in bone mechanical properties induced by immobilization. The effect of IG9402 administration was also tested in a mouse model of masticatory hypofunction through the induction of masseter muscle atrophy by unilateral injection of botulinum toxin type A (BoNTA). IG9402 partially inhibited the loss of trabecular bone microstructure in the mandibular condyle, but not the decrease in masseter muscle mass induced by BoNTA administration. In summary, these non-traditional bisphosphonates that lack anti-resorptive activity but are able to preserve osteoblast and osteocyte viability could constitute useful tools to study the consequences of preventing apoptosis of osteoblastic cells in animal models. Furthermore, they could be used to treat conditions associated with reduced bone mass and increased bone fragility in which a reduction of bone remodeling is not desirable.
Lilian I. Plotkin; Sonja Buvinic; Julián Balanta-Melo. In vitro and in vivo studies using non-traditional bisphosphonates. Bone 2020, 134, 115301 .
AMA StyleLilian I. Plotkin, Sonja Buvinic, Julián Balanta-Melo. In vitro and in vivo studies using non-traditional bisphosphonates. Bone. 2020; 134 ():115301.
Chicago/Turabian StyleLilian I. Plotkin; Sonja Buvinic; Julián Balanta-Melo. 2020. "In vitro and in vivo studies using non-traditional bisphosphonates." Bone 134, no. : 115301.
The injection of botulinum toxin type A (BoNT/A) in the masticatory muscles, to cause its temporary paralysis, is a widely used intervention for clinical disorders such as oromandibular dystonia, sleep bruxism, and aesthetics (i.e., masseteric hypertrophy). Considering that muscle contraction is required for mechano-transduction to maintain bone homeostasis, it is relevant to address the bone adverse effects associated with muscle condition after this intervention. Our aim is to condense the current and relevant literature about mandibular bone loss in fully mature mammals after BoNT/A intervention in the masticatory muscles. Here, we compile evidence from animal models (mice, rats, and rabbits) to clinical studies, demonstrating that BoNT/A-induced masticatory muscle atrophy promotes mandibular bone loss. Mandibular bone-related adverse effects involve cellular and metabolic changes, microstructure degradation, and morphological alterations. While bone loss has been detected at the mandibular condyle or alveolar bone, cellular and molecular mechanisms involved in this process must still be elucidated. Further basic research could provide evidence for designing strategies to control the undesired effects on bone during the therapeutic use of BoNT/A. However, in the meantime, we consider it essential that patients treated with BoNT/A in the masticatory muscles be warned about a putative collateral mandibular bone damage.
Julián Balanta-Melo; Viviana Toro-Ibacache; Kornelius Kupczik; Sonja Buvinic. Mandibular Bone Loss after Masticatory Muscles Intervention with Botulinum Toxin: An Approach from Basic Research to Clinical Findings. Toxins 2019, 11, 84 .
AMA StyleJulián Balanta-Melo, Viviana Toro-Ibacache, Kornelius Kupczik, Sonja Buvinic. Mandibular Bone Loss after Masticatory Muscles Intervention with Botulinum Toxin: An Approach from Basic Research to Clinical Findings. Toxins. 2019; 11 (2):84.
Chicago/Turabian StyleJulián Balanta-Melo; Viviana Toro-Ibacache; Kornelius Kupczik; Sonja Buvinic. 2019. "Mandibular Bone Loss after Masticatory Muscles Intervention with Botulinum Toxin: An Approach from Basic Research to Clinical Findings." Toxins 11, no. 2: 84.
Background: Mouse molar is a widely used model for teeth development. However, the effect of masticatory function on enamel and dentine in adult individuals remains poorly understood. As reported, the unilateral masseter hypofunction induced by botulinum toxin type A (BoNTA) resulted in mandibular bone damage and signs of unilateral chewing in adult mice. Objective: We aimed to assess the amount of enamel and dentine in the first molar (M1) during the unilateral masseter hypofunction in mice, using high-resolution X-ray microtomography (μCT) as threedimensional approach. Materials and methods: Mandibles of adult BALB/c mice, located either in a Control-group (without intervention) or a BoNTA-group, were ex-vivo scanned using μCT. Treated individuals received each one BoNTA intervention in the right masseter, and saline solution in the left masseter (intra-individual control). Enamel and dentine from M1 were segmented, and volume, thickness and mesial root length were quantified. Results: Enamel volume from treated side resulted unchanged after 2 weeks of unilateral masseter hypofunction. No differences for enamel volume were found between both sides of control individuals, and between these and samples from hypofunctional side in BoNTA-group. Enamel volume from saline-injected side was reduced when compared with experimental side (p<0,01). No differences in dentine volume, thickness of enamel and dentine, and mesial root length were found for any group. Conclusion: The amount of enamel in hypofunctional molars remains unaffected after unilateral BoNTA intervention in the masseter, but contralateral side showed reduced enamel volume. Therefore, increased functional wearing during unilateral chewing after BoNTA intervention should be considered.
Julián Balanta-Melo; Maximilian Bemmann; Viviana Toro Ibacache; Kornelius Kupczik; Sonja Buvinic. Three-dimensional assessment of enamel and dentine in mouse molar teeth during masseter muscle hypofunction. Revista Estomatología 2018, 26, 30 -37.
AMA StyleJulián Balanta-Melo, Maximilian Bemmann, Viviana Toro Ibacache, Kornelius Kupczik, Sonja Buvinic. Three-dimensional assessment of enamel and dentine in mouse molar teeth during masseter muscle hypofunction. Revista Estomatología. 2018; 26 (2):30-37.
Chicago/Turabian StyleJulián Balanta-Melo; Maximilian Bemmann; Viviana Toro Ibacache; Kornelius Kupczik; Sonja Buvinic. 2018. "Three-dimensional assessment of enamel and dentine in mouse molar teeth during masseter muscle hypofunction." Revista Estomatología 26, no. 2: 30-37.
Masseter muscle function influences mandibular bone homeostasis. As previously reported, bone resorption markers increased in the mouse mandibular condyle two days after masseter paralysis induced with botulinum toxin type A (BoNTA), followed by local bone loss. This study aimed to evaluate the bone quality of both the mandibular condyle and alveolar process in the mandible of adult mice during the early stage of a BoNTA-induced masseter muscle atrophy, using a combined 3D histomorphometrics and shape analysis approach. Adult BALB/c mice were divided into an untreated control group and an experimental group; the latter received one single BoNTA injection in the right masseter (BoNTA-right) and saline in the left masseter (Saline-left). 3D bone microstructural changes in the mandibular condyle and alveolar process were determined with high-resolution microtomography. Additionally, landmark-based geometric morphometrics was implemented to assess external shape changes. After 2 weeks, masseter mass was significantly reduced (P-value 0.05). Condyle bone quality deteriorates at an early stage of BoNTA-induced masseter muscle atrophy, and before the alveolar process is affected. Since the observed bone microstructural changes resemble those in human temporomandibular joint degenerative disorders, the clinical safety of BoNTA intervention in the masticatory apparatus remains to be clarified.
Julián Balanta-Melo; María Angélica Torres-Quintana; Maximilian Bemmann; Carolina Vega; Constanza González; Kornelius Kupczik; Viviana Toro-Ibacache; Sonja Buvinic. Masseter muscle atrophy impairs bone quality of the mandibular condyle but not the alveolar process early after induction. Journal of Oral Rehabilitation 2018, 46, 233 -241.
AMA StyleJulián Balanta-Melo, María Angélica Torres-Quintana, Maximilian Bemmann, Carolina Vega, Constanza González, Kornelius Kupczik, Viviana Toro-Ibacache, Sonja Buvinic. Masseter muscle atrophy impairs bone quality of the mandibular condyle but not the alveolar process early after induction. Journal of Oral Rehabilitation. 2018; 46 (3):233-241.
Chicago/Turabian StyleJulián Balanta-Melo; María Angélica Torres-Quintana; Maximilian Bemmann; Carolina Vega; Constanza González; Kornelius Kupczik; Viviana Toro-Ibacache; Sonja Buvinic. 2018. "Masseter muscle atrophy impairs bone quality of the mandibular condyle but not the alveolar process early after induction." Journal of Oral Rehabilitation 46, no. 3: 233-241.
Masseter muscle paralysis induced by botulinum toxin type A (BoNTA) evokes subchondral bone loss in mandibular heads of adult rats and growing mice after 4 weeks. However, the primary cellular and molecular events leading to altered bone remodeling remain unexplored. Thus, the aim of the current work has been to assess the molecular response that precedes the early microanatomical changes in the masseter muscle and subchondral bone of the mandibular head in adult mice after BoNTA intervention. A pre-clinical in vivo study was performed by a single intramuscular injection of 0.2 U BoNTA in the right masseter (experimental) of adult BALB/c mice. The contralateral masseter was injected with vehicle (control). Changes in mRNA levels of molecular markers of bone loss or muscle atrophy/regeneration were addressed by qPCR at day 2 or 7, respectively. mRNA levels of receptor activator of nuclear factor-κB ligand (RANKL) was assessed in mandibular heads, whilst mRNA levels of Atrogin-1/MAFbx, MuRF-1 and Myogenin were addressed in masseter muscles. In order to identify the early microanatomical changes at day 14, fiber diameters in transversal sections of masseter muscles were quantified, and histomorphometric analysis was used to determine the bone per tissue area and the trabecular thickness of subchondral bone of the mandibular heads. An increase of up to 4-fold in RANKL mRNA levels were detected in mandibular heads of the BoNTA-injected sides as early as 2 d after intervention. Moreover, a 4-6 fold increase in Atrogin-1/MAFbx and MuRF-1 and an up to 25 fold increase in myogenin mRNA level were detected in masseter muscles 7 d after BoNTA injections. Masseter muscle mass, as well as individual muscle fiber diameter, were significantly reduced in BoNTA-injected side after 14 d post-intervention. At the same time, in the mandibular heads from the treated side, the subchondral bone loss was evinced by a significant reduction in bone per tissue area (- 40%) and trabecular thickness (- 55%). Our results show that masseter muscle paralysis induced by BoNTA leads to significant microanatomical changes by day 14, preceded by molecular changes as early as 2 d in bone, and 7 d in muscle. Therefore, masseter muscle atrophy and subchondral bone loss detected at 14 d are preceded by molecular responses that occur during the first week after BoNTA intervention.
Julián Balanta-Melo; Viviana Toro-Ibacache; Maria Angélica Torres-Quintana; Kornelius Kupczik; Carolina Vega; Camilo Morales; Nadia Hernández-Moya; Manuel Arias-Calderón; Carolina Beato; Sonja Buvinic. Early molecular response and microanatomical changes in the masseter muscle and mandibular head after botulinum toxin intervention in adult mice. Annals of Anatomy - Anatomischer Anzeiger 2018, 216, 112 -119.
AMA StyleJulián Balanta-Melo, Viviana Toro-Ibacache, Maria Angélica Torres-Quintana, Kornelius Kupczik, Carolina Vega, Camilo Morales, Nadia Hernández-Moya, Manuel Arias-Calderón, Carolina Beato, Sonja Buvinic. Early molecular response and microanatomical changes in the masseter muscle and mandibular head after botulinum toxin intervention in adult mice. Annals of Anatomy - Anatomischer Anzeiger. 2018; 216 ():112-119.
Chicago/Turabian StyleJulián Balanta-Melo; Viviana Toro-Ibacache; Maria Angélica Torres-Quintana; Kornelius Kupczik; Carolina Vega; Camilo Morales; Nadia Hernández-Moya; Manuel Arias-Calderón; Carolina Beato; Sonja Buvinic. 2018. "Early molecular response and microanatomical changes in the masseter muscle and mandibular head after botulinum toxin intervention in adult mice." Annals of Anatomy - Anatomischer Anzeiger 216, no. : 112-119.
Julián Balanta-Melo; Sonja Buvinic. Mandibular bone loss: a hidden side effect of botulinum toxin type A injection in masticatory muscles. Journal of Oral Research 2018, 7, 44 -46.
AMA StyleJulián Balanta-Melo, Sonja Buvinic. Mandibular bone loss: a hidden side effect of botulinum toxin type A injection in masticatory muscles. Journal of Oral Research. 2018; 7 (2):44-46.
Chicago/Turabian StyleJulián Balanta-Melo; Sonja Buvinic. 2018. "Mandibular bone loss: a hidden side effect of botulinum toxin type A injection in masticatory muscles." Journal of Oral Research 7, no. 2: 44-46.
Objective: The aim of this study is to identify the craniofacial changes of long term use of Mandibular Advancement Devices (MAD) for Obstructive Sleep Apnea (OSA) treatment trough a systematic review of the literature. Materials and methods: A systematic review was performed assessing the following electronic databases: PubMed, EBSCOhost, The Cochrane Library and EMBASE,between January 2000 and May 2014, human studies and english language. Inclusion and exclusion criteria were applied to the results. Selected articles were evaluated with the use of an international check list related to the type of the study (CONSORT). A classification of the level of evidence and the degree of recommendation were performed trough the SORT strategy. Results: 1383 articles were identified after the initial search. After applying the criteria selection and complete the check list selected (CONSORT) only 1 study fulfilled the inclusion criteria. A classification of the level of evidence and grade of recommendation were performed by using the SORT strategy to the selected article. Conclusions: No enough body of evidence were found in this study to identify the craniofacial changes with the long term use of Mandibular Advancement Devices for the treatment of Obstructive Sleep Apnea.
Julian Balanta-Melo. Long-term use of mandibular advancement devices for the treatment of obstructive sleep apnea: A systematic review of craniofacial changes. Revista Estomatología 2015, 22, 35 -45.
AMA StyleJulian Balanta-Melo. Long-term use of mandibular advancement devices for the treatment of obstructive sleep apnea: A systematic review of craniofacial changes. Revista Estomatología. 2015; 22 (2):35-45.
Chicago/Turabian StyleJulian Balanta-Melo. 2015. "Long-term use of mandibular advancement devices for the treatment of obstructive sleep apnea: A systematic review of craniofacial changes." Revista Estomatología 22, no. 2: 35-45.