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Min Feng
Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

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Journal article
Published: 06 May 2021 in Viruses
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Viruses rely on host cell metabolism to provide the necessary energy and biosynthetic precursors for successful viral replication. Infection of the silkworm, Bombyx mori, by Bombyx mori nucleopolyhedrovirus (BmNPV), has been studied extensively in the past to unravel interactions between baculoviruses and their lepidopteran hosts. To understand the interaction between the host metabolic responses and BmNPV infection, we analyzed global metabolic changes associated with BmNPV infection in silkworm hemolymph. Our metabolic profiling data suggests that amino acid metabolism is strikingly altered during a time course of BmNPV infection. Amino acid consumption is increased during BmNPV infection at 24 h post infection (hpi), but their abundance recovered at 72 hpi. Central carbon metabolism, on the other hand, particularly glycolysis and glutaminolysis, did not show obvious changes during BmNPV infection. Pharmacologically inhibiting the glycolytic pathway and glutaminolysis also failed to reduce BmNPV replication, revealing that glycolysis and glutaminolysis are not essential during BmNPV infection. This study reveals a unique amino acid utilization process that is implemented during BmNPV infection. Our metabolomic analysis of BmNPV-infected silkworm provides insights as to how baculoviruses induce alterations in host metabolism during systemic infection.

ACS Style

Min Feng; Shigang Fei; Junming Xia; Mengmeng Zhang; Hongyun Wu; Luc Swevers; Jingchen Sun. Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism. Viruses 2021, 13, 841 .

AMA Style

Min Feng, Shigang Fei, Junming Xia, Mengmeng Zhang, Hongyun Wu, Luc Swevers, Jingchen Sun. Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism. Viruses. 2021; 13 (5):841.

Chicago/Turabian Style

Min Feng; Shigang Fei; Junming Xia; Mengmeng Zhang; Hongyun Wu; Luc Swevers; Jingchen Sun. 2021. "Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism." Viruses 13, no. 5: 841.

Journal article
Published: 05 February 2021 in International Journal of Biological Macromolecules
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The viruses utilize multiple cellular proteins to facilitate their proliferation. The Heat Shock Protein (HSP), the highly conserved protein in eukaryotes and prokaryotes, plays a critical role in facilitating viral proliferation. However, less is known about the role of the HSPs in the life cycles of the Baculoviruses. We constructed recombinant Bombyx mori nucleopolyhedrovirus and discovered the Heat Shock Protein 75 (TRAP1) in the B. mori ovary (BmN) cells by the co-immunoprecipitation experiment using the GP64 (glycoprotein 64) as the bait protein. Tissue expression profile analysis of B. mori indicated that the TRAP1 gene has higher expression levels in the ovary, midgut, and hemolymph. Down-regulation of TRAP1 via RNA interference (RNAi) and geldanamycin (GA, a TRAP1 inhibitor) treatment can reduce the expression level of the major capsid protein VP39 (viral protein 39) of BmNPV. In contrast, the up-regulation of TRAP1 via overexpression can increase the expression level of the VP39. These results indicated that the TRAP1 of B. mori could facilitate the proliferation of the BmNPV. This study provided new insights into the function of TRAP1, and the basic mechanisms of the baculoviruses life cycle for disease prevention.

ACS Style

Xiong Wang; Yinong Zhang; Shigang Fei; Mian Muhammad Awais; Hao Zheng; Min Feng; Jingchen Sun. Heat Shock Protein 75 (TRAP1) facilitate the proliferation of the Bombyx mori nucleopolyhedrovirus. International Journal of Biological Macromolecules 2021, 175, 372 -378.

AMA Style

Xiong Wang, Yinong Zhang, Shigang Fei, Mian Muhammad Awais, Hao Zheng, Min Feng, Jingchen Sun. Heat Shock Protein 75 (TRAP1) facilitate the proliferation of the Bombyx mori nucleopolyhedrovirus. International Journal of Biological Macromolecules. 2021; 175 ():372-378.

Chicago/Turabian Style

Xiong Wang; Yinong Zhang; Shigang Fei; Mian Muhammad Awais; Hao Zheng; Min Feng; Jingchen Sun. 2021. "Heat Shock Protein 75 (TRAP1) facilitate the proliferation of the Bombyx mori nucleopolyhedrovirus." International Journal of Biological Macromolecules 175, no. : 372-378.

Journal article
Published: 24 May 2020 in Veterinary Research
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Interferon-stimulated genes (ISGs) play an important role in antiviral innate immune responses. Although many ISGs have been identified in mammals, researchers commonly recognize that many more ISGs are yet to be discovered. Current information is still very limited particularly for the systematic identification of type III ISGs. Similarly, current research on ISGs in birds is still in its infancy. The aim of this study was to systematically identify chicken type I (IFN-α), II (IFN-γ) and III (IFN-λ) ISGs and analyze their respective response elements. RNA sequencing (RNA-Seq) was employed to identify those genes with up-regulated expression following chicken IFN-α, IFN-γ and IFN-λ treatment. Two hundred and five type I ISGs, 299 type II ISGs, and 421 type III ISGs were identified in the chicken. We further searched for IFN-stimulated response elements (ISRE) and gamma-activated sequences (GAS) elements in the promoters region of ISGs. The GAS elements were common in the promoter of type II ISGs and were even detected in type I and III ISGs. However, ISRE were not commonly found in the promoters of chicken ISGs. Furthermore, we demonstrated that ISRE in chicken cells were significantly activated by IFN-α or IFN-λ treatment, and expectedly, that GAS elements were also significantly activated by IFN-γ treatment. Interestingly, we also found that GAS elements were significantly activated by IFN-λ. Our study provides a systematic library of ISGs in the chicken together with preliminary information about the transcriptional regulation of the identified ISGs.

ACS Style

Manman Dai; Tingting Xie; Ming Liao; Xiquan Zhang; Min Feng. Systematic identification of chicken type I, II and III interferon-stimulated genes. Veterinary Research 2020, 51, 1 -12.

AMA Style

Manman Dai, Tingting Xie, Ming Liao, Xiquan Zhang, Min Feng. Systematic identification of chicken type I, II and III interferon-stimulated genes. Veterinary Research. 2020; 51 (1):1-12.

Chicago/Turabian Style

Manman Dai; Tingting Xie; Ming Liao; Xiquan Zhang; Min Feng. 2020. "Systematic identification of chicken type I, II and III interferon-stimulated genes." Veterinary Research 51, no. 1: 1-12.

Journal article
Published: 11 June 2019 in Developmental & Comparative Immunology
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Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression in infected chickens. Macrophages play a crucial role in host defense against invading pathogens. In the present study, whole transcriptome analysis was performed to analyze the host factors including genes, microRNA (miRNA), long non-coding RNA (lncRNA) and their regulatory network in chicken primary monocyte-derived macrophages (MDMs). In total, 128 differentially expressed (DE) lncRNAs and 15 DE miRNAs were identified in MDMs at 3 h post infection (hpi), and 30 DE lncRNAs and 8 DE miRNAs were identified in MDMs at 36 hpi during ALV-J infection. We further constructed the DE lncRNAs-mRNAs, miRNA-mRNAs and lncRNAs-miRNA-mRNAs interaction networks. The results suggested that DE lncRNAs and miRNAs are involved in the regulation of CCND3 and SOCS5 in Jak-STAT signaling pathway via ceRNA network in ALV-J-infected MDMs at 3 hpi. In addition, lncRNAs including XLOC_672329, ALDBGALG0000001429, XLOC_016500 and ALDBGALG0000000253 cis-regulating CH25H, CISH, IL-1β and CD80 respectively in MDMs at 3 hpi participated in host antiviral responses. Our findings give a comprehensive view of the connection between non-coding RNA and ALV-J in chicken primary macrophages, and provide an excellent resource for further studies of epigenetic effects on ALV-J disease resistance breeding as well as immune system and genomic researches.

ACS Style

Manman Dai; Min Feng; Tingting Xie; Xiquan Zhang. Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage. Developmental & Comparative Immunology 2019, 100, 103414 .

AMA Style

Manman Dai, Min Feng, Tingting Xie, Xiquan Zhang. Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage. Developmental & Comparative Immunology. 2019; 100 ():103414.

Chicago/Turabian Style

Manman Dai; Min Feng; Tingting Xie; Xiquan Zhang. 2019. "Long non-coding RNA and MicroRNA profiling provides comprehensive insight into non-coding RNA involved host immune responses in ALV-J-infected chicken primary macrophage." Developmental & Comparative Immunology 100, no. : 103414.

Journal article
Published: 30 May 2019 in Viruses
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The avian leukosis virus subgroup J (ALV-J) belongs to the chicken retrovirus that causes enormous economic losses in the poultry industry. Interferon-stimulated genes (ISGs) are critical for controlling virus infections. Here, we identified 897 type I ISGs induced by interferon-α (IFN-α) in chicken peripheral blood mononuclear cell (PBMC) by RNA-Seq. In addition, we further identified 152 potential anti-ALV-J chicken type I ISGs. Among these potential anti-ALV-J ISGs, chicken cholesterol 25-hydroxylase (chCH25H) was selected for further antiviral mechanism studies in chicken embryo fibroblast cell lines (DF1). The gene chCH25H is located on chromosome 6 and clustered in a distinct group with mammals CH25H in the phylogenetic tree. The core promoter region of chCH25H was located within −75/−1 sequence. We found that chCH25H was induced by chicken IFN-α and ALV-J in DF1 cells. The overexpression of chCH25H significantly inhibited ALV-J replication in DF1 cells at 48 h post infection (hpi). In addition, ALV-J replication was significantly enhanced in the chCH25H- knockout DF1 cells. Furthermore, we demonstrated that chCH25H restricted ALV-J infection through the production of 25-hydroxycholesterol (25HC), rather than type I and II interferon. Our results identified 152 potential anti-ALV-J chicken type I ISGs and revealed that 25HC, the product of chCH25H, could be used as a natural antiviral agent to control ALV-J infection.

ACS Style

Tingting Xie; Min Feng; Manman Dai; Guodong Mo; Zhuohao Ruan; Guiyan Wang; Meiqing Shi; Xiquan Zhang. Cholesterol-25-hydroxylase Is a Chicken ISG That Restricts ALV-J Infection by Producing 25-hydroxycholesterol. Viruses 2019, 11, 498 .

AMA Style

Tingting Xie, Min Feng, Manman Dai, Guodong Mo, Zhuohao Ruan, Guiyan Wang, Meiqing Shi, Xiquan Zhang. Cholesterol-25-hydroxylase Is a Chicken ISG That Restricts ALV-J Infection by Producing 25-hydroxycholesterol. Viruses. 2019; 11 (6):498.

Chicago/Turabian Style

Tingting Xie; Min Feng; Manman Dai; Guodong Mo; Zhuohao Ruan; Guiyan Wang; Meiqing Shi; Xiquan Zhang. 2019. "Cholesterol-25-hydroxylase Is a Chicken ISG That Restricts ALV-J Infection by Producing 25-hydroxycholesterol." Viruses 11, no. 6: 498.

Journal article
Published: 06 May 2019 in Viruses
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The published genome sequence of Antheraea yamamai (Saturnnidae) was used to construct a library of long terminal repeat (LTR)-retrotransposons that is representative of the wild silkmoth (Antherea) genus, and that includes 22,666 solo LTRs and 541 full-length LTRs. The LTR retrotransposons of Antheraea yamamai (AyLTRs) could be classified into the three canonical groups of Gypsy, Copia and Belpao. Eleven AyLTRs contained the env gene element, but the relationship with the env element of baculovirus, particularly A. yamamai and pernyi nucleopolyhedrovirus (AyNPV and ApNPV), was distant. A total of 251 “independent” full-length AyLTRs were identified that were located within 100 kb distance (downstream or upstream) of 406 neighboring genes in A. yamamai. Regulation of these genes might occur in cis by the AyLTRs, and the neighboring genes were found to be enriched in GO terms such as “response to stimulus”, and KEGG terms such as “mTOR signaling pathway” among others. Furthermore, the library of LTR-retrotransposons and the A. yamamai genome were used to identify and analyze the expression of LTR-retrotransposons and genes in ApNPV-infected and non-infected A. pernyi larval midguts, using raw data of a published transcriptome study. Our analysis demonstrates that 93 full-length LTR-retrotransposons are transcribed in the midgut of A. pernyi of which 12 significantly change their expression after ApNPV infection (differentially expressed LTR-retrotransposons or DELs). In addition, the expression of differentially expressed genes (DEGs) and neighboring DELs on the chromosome following ApNPV infection suggests the possibility of regulation of expression of DEGs by DELs through a cis mechanism, which will require experimental verification. When examined in more detail, it was found that genes involved in Notch signaling and stress granule (SG) formation were significantly up-regulated in ApNPV-infected A. pernyi larval midgut. Moreover, several DEGs in the Notch and SG pathways were found to be located in the neighborhood of particular DELs, indicating the possibility of DEG-DEL cross-regulation in cis for these two pathways.

ACS Style

Min Feng; Feifei Ren; Yaohong Zhou; Nan Zhang; Qiuyuan Lu; Luc Swevers; Jingchen Sun. Correlation in Expression between LTR Retrotransposons and Potential Host Cis-Targets during Infection of Antherea pernyi with ApNPV Baculovirus. Viruses 2019, 11, 421 .

AMA Style

Min Feng, Feifei Ren, Yaohong Zhou, Nan Zhang, Qiuyuan Lu, Luc Swevers, Jingchen Sun. Correlation in Expression between LTR Retrotransposons and Potential Host Cis-Targets during Infection of Antherea pernyi with ApNPV Baculovirus. Viruses. 2019; 11 (5):421.

Chicago/Turabian Style

Min Feng; Feifei Ren; Yaohong Zhou; Nan Zhang; Qiuyuan Lu; Luc Swevers; Jingchen Sun. 2019. "Correlation in Expression between LTR Retrotransposons and Potential Host Cis-Targets during Infection of Antherea pernyi with ApNPV Baculovirus." Viruses 11, no. 5: 421.

Journal article
Published: 06 March 2019 in Veterinary Research
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Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression in infected chickens. Macrophages play a central role in host defense against invading pathogens. In this study, we discovered an interesting phenomenon: ALV-J replication is weakened from 3 hours post-infection (hpi) to 36 hpi, which was verified using Western blotting and RT-PCR. To further investigate the interaction between ALV-J and macrophages, transcriptome analysis was performed to analyze the host genes’ function in chicken primary monocyte-derived macrophages (MDM). Compared to the uninfected control, 624 up-regulated differentially expressed genes (DEG) and 341 down-regulated DEG at 3 hpi, and 174 up-regulated DEG and 87 down-regulated DEG at 36 hpi were identified in chicken MDM, respectively. ALV-J infection induced strong innate immune responses in chicken MDM at 3 hpi, instead of 36 hpi, according to the analysis results of Gene Ontology and KEGG pathway. Importantly, the host factors, such as up-regulated MIP-3α, IL-1β, iNOS, K60, IRG1, CH25H, NFKBIZ, lysozyme and OASL were involved in the host defense response during the course of ALV-J infection. On the contrary, up-regulated EX-FABP, IL4I1, COX-2, NFKBIA, TNFAIP3 and the Jak STAT pathway inhibitors including CISH, SOCS1 and SOCS3 are beneficial to ALV-J survival in chicken macrophages. We speculated that ALV-J tropism for macrophages helps to establish a latent infection in chicken MDM from 6 to 36 hpi. The present study provides a comprehensive view of the interactions between macrophages and ALV-J. It suggests the mechanisms of defense of chicken macrophages against ALV-J invasion and how ALV-J escape the host innate immune responses.

ACS Style

Min Feng; Tingting Xie; Yuanfang Li; Nan Zhang; Qiuyuan Lu; Yaohong Zhou; Meiqing Shi; Jingchen Sun; Xiquan Zhang. A balanced game: chicken macrophage response to ALV-J infection. Veterinary Research 2019, 50, 20 .

AMA Style

Min Feng, Tingting Xie, Yuanfang Li, Nan Zhang, Qiuyuan Lu, Yaohong Zhou, Meiqing Shi, Jingchen Sun, Xiquan Zhang. A balanced game: chicken macrophage response to ALV-J infection. Veterinary Research. 2019; 50 (1):20.

Chicago/Turabian Style

Min Feng; Tingting Xie; Yuanfang Li; Nan Zhang; Qiuyuan Lu; Yaohong Zhou; Meiqing Shi; Jingchen Sun; Xiquan Zhang. 2019. "A balanced game: chicken macrophage response to ALV-J infection." Veterinary Research 50, no. 1: 20.

Original article
Published: 18 September 2018 in Molecular Genetics and Genomics
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Bombyx mori Nucleopolyhedrovirus (BmNPV), which is a member of the Baculoviridae family, is a significant pathogen of the silkworm. The infection of BmNPV is often lethal and causes about 20% loss of cocoon in the silk industry annually. To explore the effects of different gene inhibition strategies on the replication cycle of baculovirus, we constructed the mutant virus to infect BmN cells directly and further identified ie0, ie1, and gp64 as the essential viral genes of BmNPV. To elucidate the significance of the inhibition effect of different interference strategies, we characterized and constructed the recombinant BmNPV that carried a single or multigene-interfering cassette. The results showed that the inhibition effect of dsie1 on target gene expression, virus titer, and silkworm mortality was significantly better than that of dsie0 and dsgp64. It also showed that the dsie1 interference produced fewer progeny virions and was less lethal, which indicates that ie1 played a more critical role in the BmNPV replication cycle. Furthermore, the inhibitory effect of the virus titer and mortality indicated that the multigene co-interference constructed by the baculovirus expression system was significantly better than the interference of any single-gene (p < 0.05). In summary, the strategy of multigene synergy can achieve the function of continuous interference and provide a new platform for the breeding of silkworm disease resistant. In addition, this strategy improves the various traits of the silkworm.

ACS Style

Hao Zheng; Feifei Ren; Qiuyuan Lu; Zhenming Cao; Jichen Song; Min Feng; Jisheng Liu; Jingchen Sun. An efficient method for multigene co-interference by recombinant Bombyx mori nucleopolyhedrovirus. Molecular Genetics and Genomics 2018, 294, 111 -120.

AMA Style

Hao Zheng, Feifei Ren, Qiuyuan Lu, Zhenming Cao, Jichen Song, Min Feng, Jisheng Liu, Jingchen Sun. An efficient method for multigene co-interference by recombinant Bombyx mori nucleopolyhedrovirus. Molecular Genetics and Genomics. 2018; 294 (1):111-120.

Chicago/Turabian Style

Hao Zheng; Feifei Ren; Qiuyuan Lu; Zhenming Cao; Jichen Song; Min Feng; Jisheng Liu; Jingchen Sun. 2018. "An efficient method for multigene co-interference by recombinant Bombyx mori nucleopolyhedrovirus." Molecular Genetics and Genomics 294, no. 1: 111-120.

Journal article
Published: 24 November 2016 in Microbial Pathogenesis
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We have previously shown that the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway contributes to subgroup J avian leukosis virus (ALV-J) replication and tumorigenicity. However, a role for ERK/MAPK signaling in ALV-A and ALV-B replication is unknown. In this study we successfully constructed and recovered a recombinant form of ALV-A strain GD13-1 which showed similarities in growth to the parental wild type virus in vitro. ALV subgroups J, A or B all triggered ERK2 activation in primary CEF cells. ERK/MAPK inhibition markedly suppressed ALV-A and ALV-B replication as shown by extremely low levels of viral transcription and virus protein production. This finding provides evidence that ERK/MAPK signaling responses play important roles in ALV replication and may represent novel drug targets for therapeutic intervention strategies.

ACS Style

Manman Dai; Min Feng; Ming Liao; Weisheng Cao. Inhibition of ERK/MAPK suppresses avian leukosis virus subgroup A and B replication. Microbial Pathogenesis 2016, 102, 29 -35.

AMA Style

Manman Dai, Min Feng, Ming Liao, Weisheng Cao. Inhibition of ERK/MAPK suppresses avian leukosis virus subgroup A and B replication. Microbial Pathogenesis. 2016; 102 ():29-35.

Chicago/Turabian Style

Manman Dai; Min Feng; Ming Liao; Weisheng Cao. 2016. "Inhibition of ERK/MAPK suppresses avian leukosis virus subgroup A and B replication." Microbial Pathogenesis 102, no. : 29-35.

Journal article
Published: 03 April 2015 in Virology Journal
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Subgroup A, B, and J ALVs are the most prevalent avian leukosis virus (ALV). Our study attempted to develop two SYBR Green I-based real-time PCR (RT-PCR) assays for specific detection of ALV subgroup J (ALV-J) and multiplex detection of ALV subgroups A and B (ALV-A/B), respectively. The two assays showed high specificity for ALV-J and ALV-A/B and the sensitivity of the two assays was at least 100 times higher than that of the routine PCR assay. The minimum virus detection limit of virus culture, routine PCR and real-time PCR for detection of ALV-A strain was 103 TCID50 units, 102 TCID50 units and fewer than 10 TCID50 units, respectively. In addition, the coefficients of variation for intra- and inter-assay were both less than 5%. Forty clinical plasma samples were evaluated by real-time PCR, routine PCR, and virus culture with positive rates of 80% (32/40), 72.5% (29/40) and 62.5% (25/40), respectively. When the assay for detection of ALV-J was used to quantify the viral load of various organ tissues in chicken inoculated by ALV-J strains CHN06 and NX0101, the results exhibited that ALV-J genes could be detected in all organ tissues examined and the highest copies of ALV-J were mainly in heart and kidney samples at 30 weeks post-infection. Except in lung, the virus copies of CHN06 group were higher than that of NX0101 group in various organ tissues. The SYBR Green I-based real-time RT-PCR assay provides a powerful tool for the detection of ALV and study of virus replication and infection.

ACS Style

Manman Dai; Min Feng; Di Liu; Weisheng Cao; Ming Liao. Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B. Virology Journal 2015, 12, 1 -10.

AMA Style

Manman Dai, Min Feng, Di Liu, Weisheng Cao, Ming Liao. Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B. Virology Journal. 2015; 12 (1):1-10.

Chicago/Turabian Style

Manman Dai; Min Feng; Di Liu; Weisheng Cao; Ming Liao. 2015. "Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B." Virology Journal 12, no. 1: 1-10.