This page has only limited features, please log in for full access.

Unclaimed
Ke-Ping Chen
School of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, P. R. China

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 23 August 2021 in Processes
Reads 0
Downloads 0

The BbRib gene participates in the infection process of Beauveria bassiana (B. bassiana). It also helps pathogenic fungi to escape and defeat the insect host immune defense system by regulating the innate immune response. However, model insects are rarely used to study the mechanism of fungal ribosomal toxin protein. In this study, BbRib protein was produced by prokaryotic expression and injected into silkworm (Bombyx mori) larvae. The physiological and biochemical indexes of silkworm were monitored, and the pathological effects of BbRib protein on immune tissues of silkworm were examined by Hematoxylin and Eosin (HE) staining. BbRib protein can significantly affect the growth and development of the silkworm, causing poisoning, destroying the midgut and fat body and producing physiological changes. The ROS stress response in the adipose tissue and cells of the silkworm was activated to induce apoptosis. These results indicated that the BbRib gene not only participates in the infection process of B. bassiana, it also helps the pathogenic fungi escape the immune system by regulating the innate immune system of the silkworm, allowing it to break through the silkworm’s immune defense. This study reveals the potential molecular mechanism of BbRib protein to insect toxicity, and provides a theoretical basis and material basis for the development and use of novel insecticidal toxins.

ACS Style

Xiaoke Ma; Qi Ge; Rehab Hosny Taha; Keping Chen; Yi Yuan. Beauveria bassiana Ribotoxin (BbRib) Induces Silkworm Cell Apoptosis via Activating Ros Stress Response. Processes 2021, 9, 1470 .

AMA Style

Xiaoke Ma, Qi Ge, Rehab Hosny Taha, Keping Chen, Yi Yuan. Beauveria bassiana Ribotoxin (BbRib) Induces Silkworm Cell Apoptosis via Activating Ros Stress Response. Processes. 2021; 9 (8):1470.

Chicago/Turabian Style

Xiaoke Ma; Qi Ge; Rehab Hosny Taha; Keping Chen; Yi Yuan. 2021. "Beauveria bassiana Ribotoxin (BbRib) Induces Silkworm Cell Apoptosis via Activating Ros Stress Response." Processes 9, no. 8: 1470.

Journal article
Published: 23 August 2021 in Processes
Reads 0
Downloads 0

The silkworm, Bombyx mori, uses a complex olfactory system to determine whether the food is edible. As an odor degrading enzyme, UDP-glycosyltransferase (UGT) participates in the degradation of odor molecules in the olfactory system of the silkworm. By sequencing the whole genome of the silkworm NB and using comparative genomics methods, we found that UGT46A1 is unique in species that eat mulberry leaves. Bioinformatics shows that its function may be related to the feeding habits of the silkworm. In this study, it was found through quantitative real-time polymerase chain reaction (qRT-PCR) that UGT46A1 was highly expressed in the heads of silkworms, which was consistent with the conjecture that UGT46A1 was involved in silkworm olfactory recognition. RNA interference (RNAi) was used to knock down the expression of UGT46A1. By observing the silkworm’s tendency toward mulberry leaves and food selectivity, it was found that the silkworms that successfully knocked down the UGT46A1 gene altered their feeding habits and that their ability to find food was weakened, but they could eat more leaves of plants other than mulberry leaves. This evidence indicates that UGT46A1 may affect the silkworm’s feeding by influencing the olfactory system of the silkworm.

ACS Style

Wenting Song; Yixuan Fan; Feifei Zhu; Rehab Hosny Taha; Keping Chen. The Expression of UGT46A1 Gene and Its Effect on Silkworm Feeding. Processes 2021, 9, 1473 .

AMA Style

Wenting Song, Yixuan Fan, Feifei Zhu, Rehab Hosny Taha, Keping Chen. The Expression of UGT46A1 Gene and Its Effect on Silkworm Feeding. Processes. 2021; 9 (8):1473.

Chicago/Turabian Style

Wenting Song; Yixuan Fan; Feifei Zhu; Rehab Hosny Taha; Keping Chen. 2021. "The Expression of UGT46A1 Gene and Its Effect on Silkworm Feeding." Processes 9, no. 8: 1473.

Review article
Published: 19 August 2021 in Journal of Asia-Pacific Entomology
Reads 0
Downloads 0

Food is critical to the survival and development of insects. Bombyx mori (Lepidoptera: Bombycidae), a Lepidoptera model insect with economic significance, is a well-known oligophagous insect that mainly feeds on mulberry leaves. The feeding characteristics of this particular species provide an excellent model for studying the food selection of insect host plants. In recent years, there has been an increasing number of studies on the factors affecting the feeding of silkworms, especially with the development of molecular technology. Many mysteries have been gradually solved. This paper summarizes and discusses the factors that affect the feeding of silkworms, with a focus on the molecular mechanisms of silkworm feeding. It is hoped that this work will be helpful for further research on artificial diets for silkworms and disease control in lepidopteran insects.

ACS Style

Wen-Ting Song; Fei-Fei Zhu; Ke-Ping Chen. The molecular mechanisms and factors affecting the feeding habits of silkworm (Lepidoptera: Bombyxidae). Journal of Asia-Pacific Entomology 2021, 1 .

AMA Style

Wen-Ting Song, Fei-Fei Zhu, Ke-Ping Chen. The molecular mechanisms and factors affecting the feeding habits of silkworm (Lepidoptera: Bombyxidae). Journal of Asia-Pacific Entomology. 2021; ():1.

Chicago/Turabian Style

Wen-Ting Song; Fei-Fei Zhu; Ke-Ping Chen. 2021. "The molecular mechanisms and factors affecting the feeding habits of silkworm (Lepidoptera: Bombyxidae)." Journal of Asia-Pacific Entomology , no. : 1.

Journal article
Published: 19 July 2021 in Processes
Reads 0
Downloads 0

Diabetes mellitus (DM), as a chronic disease caused by insulin deficiency or using obstacles, is gradually becoming a principal worldwide health problem. Pueraria lobata is one of the traditional Chinese medicinal and edible plants, playing roles in improving the cardiovascular system, lowering blood sugar, anti-inflammation, anti-oxidation, and so on. Studies on the hypoglycemic effects of Pueraria lobata were also frequently reported. To determine the active ingredients and related targets of Pueraria lobata for DM, 256 metabolites were identified by LC/MS non targeted metabonomics, and 19 active ingredients interacting with 51 DM-related targets were screened. The results showed that puerarin, quercetin, genistein, daidzein, and other active ingredients in Pueraria lobata could participate in the AGE-RAGE signaling pathway, insulin resistance, HIF-1 signaling pathway, FoxO signaling pathway, and MAPK signaling pathway by acting on VEGFA, INS, INSR, IL-6, TNF and AKT1, and may regulate type 2 diabetes, inflammation, atherosis and diabetes complications, such as diabetic retinopathy, diabetic nephropathy, and diabetic cardiomyopathy.

ACS Style

Shu Zhang; Qi Ge; Liang Chen; Keping Chen. Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology. Processes 2021, 9, 1245 .

AMA Style

Shu Zhang, Qi Ge, Liang Chen, Keping Chen. Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology. Processes. 2021; 9 (7):1245.

Chicago/Turabian Style

Shu Zhang; Qi Ge; Liang Chen; Keping Chen. 2021. "Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology." Processes 9, no. 7: 1245.

Journal article
Published: 30 June 2021 in Ecotoxicology and Environmental Safety
Reads 0
Downloads 0

Bacterial contamination has caused a major public health problem worldwide. Bacillus cereus is a conditional environmental pathogenic bacteria that can cause food poisoning. Whether environmental pathogens can cause widespread transmission in the insect kingdom is unclear. In this study, a Bacillus cereus ZJ-4 was isolated from the hospital environment of Zhenjiang City, Jiangsu Province, China. It was fatal by injection into the silkworm hemolymph. To investigated the potential toxic factors of ZJ-4 and clarified the toxicity response mechanism of silkworm by the ZJ-4 infection. Then, the whole genome of ZJ-4 was sequenced, and the immune mechanism of silkworm fat body to ZJ-4 pathogen was studied by HE pathological section and proteomics. Bacterial genome sequencing indicated that ZJ-4 had 352 drug resistance genes and 6 virulence genes. After 36 h of subcutaneous puncture with ZJ-4 suspension, the pathological changes were obviously found in HE pathological sections of fat body tissue. Comparative proteomic results indicated that differentially expressed proteins are mainly involved in stress reactions, biological regulation, and innate immunity. The qRT-PCR analysis showed that the expressions of β-GRP, Spaetzle, MyD88, Tube and Dorsal genes in Toll pathway were up-regulated, while Pell and Cactus genes were down-regulated; in the antimicrobial peptide pathway, Glv2, Lzm, Mor, and Leb3 genes were up-regulated, while attacin1 and defensin genes were down-regulated; Sod gene was up-regulated, while Cat gene was down-regulated in the antioxidant pathway; Ldh, Sdh, and Mdh genes were down-regulated in glucose metabolism pathway. These results indicated that ZJ-4 can damage the innate immune pathway of silkworm, and also affect the normal immune function of fat body cells.

ACS Style

Qi Ge; Weiping Cao; Feifei Zhu; Yi Yuan; Liang Chen; Jia Xu; Jun Li; Han Chen; Shangshang Ma; Lindan Sun; Huiwen Pan; Rehab Hosny Taha; Qin Yao; Keping Chen. Genomics and proteomics combined analysis revealed the toxicity response of silkworm Bombyx mori to the environmental pathogen Bacillus cereus ZJ-4. Ecotoxicology and Environmental Safety 2021, 222, 112467 .

AMA Style

Qi Ge, Weiping Cao, Feifei Zhu, Yi Yuan, Liang Chen, Jia Xu, Jun Li, Han Chen, Shangshang Ma, Lindan Sun, Huiwen Pan, Rehab Hosny Taha, Qin Yao, Keping Chen. Genomics and proteomics combined analysis revealed the toxicity response of silkworm Bombyx mori to the environmental pathogen Bacillus cereus ZJ-4. Ecotoxicology and Environmental Safety. 2021; 222 ():112467.

Chicago/Turabian Style

Qi Ge; Weiping Cao; Feifei Zhu; Yi Yuan; Liang Chen; Jia Xu; Jun Li; Han Chen; Shangshang Ma; Lindan Sun; Huiwen Pan; Rehab Hosny Taha; Qin Yao; Keping Chen. 2021. "Genomics and proteomics combined analysis revealed the toxicity response of silkworm Bombyx mori to the environmental pathogen Bacillus cereus ZJ-4." Ecotoxicology and Environmental Safety 222, no. : 112467.

Communication
Published: 18 April 2021 in Viruses
Reads 0
Downloads 0

Recombinant adeno-associated virus (AAV) vectors have broad application prospects in the field of gene therapy. The establishment of low-cost and large-scale manufacturing is now the general agenda for industry. The baculovirus-insect cell/larva expression system has great potential for these applications due to its scalability and predictable biosafety. To establish a more efficient production system, Bombyx mori pupae were used as a new platform and infected with recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV). The production of a chimeric recombinant adeno-associated virus (rAAV) serotype 2/human bocavirus type-1 (HBoV1) vector was used to evaluate the efficiency of this new baculovirus expression vector (BEV)–insect expression system. For this purpose, we constructed two recombinant BmNPVs, which were named rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP. The yields of rAAV2/HBoV1 derived from the rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP co-infected BmN cells exceeded 2 × 104 vector genomes (VG) per cell. The rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP can express stably for at least five passages. Significantly, rAAV2/HBoV1 could be efficiently generated from BmNPV-infected silkworm larvae and pupae at average yields of 2.52 × 1012 VG/larva and 4.6 × 1012 VG/pupa, respectively. However, the vectors produced from the larvae and pupae had a high percentage of empty particles, which suggests that further optimization is required for this platform in the future. Our work shows that silkworm pupae, as an efficient bioreactor, have great potential for application in the production of gene therapy vectors.

ACS Style

Qian Yu; Pengfei Chang; Xiaoxuan Liu; Peng Lü; Qi Tang; Zhongjian Guo; Jianming Qiu; Keping Chen; Qin Yao. Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System. Viruses 2021, 13, 704 .

AMA Style

Qian Yu, Pengfei Chang, Xiaoxuan Liu, Peng Lü, Qi Tang, Zhongjian Guo, Jianming Qiu, Keping Chen, Qin Yao. Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System. Viruses. 2021; 13 (4):704.

Chicago/Turabian Style

Qian Yu; Pengfei Chang; Xiaoxuan Liu; Peng Lü; Qi Tang; Zhongjian Guo; Jianming Qiu; Keping Chen; Qin Yao. 2021. "Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System." Viruses 13, no. 4: 704.

Preprint content
Published: 22 March 2021
Reads 0
Downloads 0

Aluminium, a substance found in large amounts in nature, has been widely used for various purposes, especially food additives. The effects of long-term and excessive exposure to aluminium on human health are receiving increasing attention. The extensive human use of aluminium food additives can also cause aluminium to enter the ecosystem, where it has significant impacts on insects. This study explored the cytotoxicity and changes in gene expression under aluminium potassium sulfate toward Spodoptera frugiperda 9 cells. We found that high concentrations of aluminium resulted in cell enlargement and cell membrane breakage, decreased cell vitality, and caused apoptosis. Through RNA-Seq transcriptomics, we found that aluminium ions may inhibit the expression of regulatory-associated protein of mTOR, tdIns-dependent protein kinase-1, and small heat shock proteins (heat shock 70 kDa protein and crystallin alpha B), leading to changes in mTOR-related pathways (such as the longevity regulation pathway and PI3K-Akt signalling pathway) , and promoting cell apoptosis. On the other hand, aluminium ions lead to the overexpression of GSH S-transferase,prostaglandin-H2 D-isomerase and pyrimidodiazepine synthase, and induce intracellular oxidative damage, which ultimately affects cell growth and apoptosis through a series of cascade reactions.

ACS Style

Rong Feng; Liang Chen; Keping Chen. Cytotoxicity and Changes in Gene Expression Under Aluminium Potassium Sulfate on Spodoptera Frugiperda 9 Cells. 2021, 1 .

AMA Style

Rong Feng, Liang Chen, Keping Chen. Cytotoxicity and Changes in Gene Expression Under Aluminium Potassium Sulfate on Spodoptera Frugiperda 9 Cells. . 2021; ():1.

Chicago/Turabian Style

Rong Feng; Liang Chen; Keping Chen. 2021. "Cytotoxicity and Changes in Gene Expression Under Aluminium Potassium Sulfate on Spodoptera Frugiperda 9 Cells." , no. : 1.

Journal article
Published: 28 February 2021 in South African Journal of Botany
Reads 0
Downloads 0

The Astragalus propinquus is used in traditional Chinese medicines (TCM) for centuries. However, its mechanism of action and functional targets remain elusive. The purpose of the study is to conduct a pharmacological network analysis to identify the interaction of A. propinquus derived components with the target proteins involved in rheumatoid arthritis (RA) and diabetes. The chemical components of A. propinquus were obtained from Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), and the target-protein related genes were collected from DisGeNET and GeneCards databases. A total of 16 A. propinquus phytochemical compounds and 147 potential targets were screened for RA and diabetes-related target proteins STRING and Cytoscape software were used to establish the protein interaction networks. Finally, the pharmacological network was integrated to investigate the mechanism underlying the potential target protein network of A. propinquus. The results showed that the A. propinquus derived components such as quercetin, hederagenin, isorhamnetin, calycosin, 7-O-methylisomucronulatol, formononetin, and kaempferol exhibited potential binding efficacy with screened 89 target proteins, suggesting it as one of the natural medicines for the treatment of RA and diabetes. Gene Ontology and KEGG analysis revealed that certain target pathways were involved in RA and diabetes, which are also elaborated in this study. The molecular docking analysis demonstrates that the compound hederagenin acts as a potent inhibitor for RA and diabetics.

ACS Style

Thasma Raman Sivakumar; Duraiarasan Surendhiran; Keping Chen; Peng Lv; Annadurai Vinothkanna; Ragothaman Prathiviraj; Sivasamy Sethupathy; Abdul Razack Sirajunnisa. Network pharmacology based analysis of Astragalus propinquus components for the treatment of rheumatoid arthritis and diabetes. South African Journal of Botany 2021, 139, 92 -105.

AMA Style

Thasma Raman Sivakumar, Duraiarasan Surendhiran, Keping Chen, Peng Lv, Annadurai Vinothkanna, Ragothaman Prathiviraj, Sivasamy Sethupathy, Abdul Razack Sirajunnisa. Network pharmacology based analysis of Astragalus propinquus components for the treatment of rheumatoid arthritis and diabetes. South African Journal of Botany. 2021; 139 ():92-105.

Chicago/Turabian Style

Thasma Raman Sivakumar; Duraiarasan Surendhiran; Keping Chen; Peng Lv; Annadurai Vinothkanna; Ragothaman Prathiviraj; Sivasamy Sethupathy; Abdul Razack Sirajunnisa. 2021. "Network pharmacology based analysis of Astragalus propinquus components for the treatment of rheumatoid arthritis and diabetes." South African Journal of Botany 139, no. : 92-105.

Journal article
Published: 04 January 2021 in Journal of Asia-Pacific Entomology
Reads 0
Downloads 0

The human macular carotenoid transporter was firstly discovered using silkworm yellow-cocoon strains, known as Bombyx mori carotenoid selective transport system (BmCSTS). However, molecular mechanisms underlying the gene/protein expression and regulations in vivo remain elusive. Here, 620 differentially expressed genes (DEGs), 1965 differentially expressed proteins (DEPs), and 67 co-genes (namely Co-DEGs-DEPs) from yellow-cocoon silkworm middle silk glands (MSGs) were identified by RNA-Seq based transcriptome and SWATH-based proteomic analysis. Notably, previously identified CBP-alike key factors were further demonstrated significantly up-regulated in all six B. mori yellow-cocoon strains (NB, NB × 306, 306 × NB, NB × 798, 798 × NB, and XH × NB) used in this study. The GO and KEGG analysis results clustered most DEGs and DEPs into multiple biological processes including both cellular components and molecular functions, especially protein synthesis and energy-metabolic pathways. The protein–protein interaction (PPI) network analysis results further revealed a predominant ribosome-biogenesis regulation working system during silkworm yellow-cocoon color formation. Additionally, high-efficient energy production and metabolism pathways (i.e. oxidative phosphorylation and citrate cycle) were also discovered, which might facilitate key gene/protein expressions and functions in BmCSTS, such as CBP and Cameo2.

ACS Style

Yi Yuan; Rui Xiao; Qi Ge; Rehab H. Taha; Keping Chen. Complementary transcriptomic and proteomic analysis of Bombyx mori middle silk glands reveals a predominant ribosome-biogenesis regulating network during silkworm yellow-cocoon color formation. Journal of Asia-Pacific Entomology 2021, 24, 260 -270.

AMA Style

Yi Yuan, Rui Xiao, Qi Ge, Rehab H. Taha, Keping Chen. Complementary transcriptomic and proteomic analysis of Bombyx mori middle silk glands reveals a predominant ribosome-biogenesis regulating network during silkworm yellow-cocoon color formation. Journal of Asia-Pacific Entomology. 2021; 24 (1):260-270.

Chicago/Turabian Style

Yi Yuan; Rui Xiao; Qi Ge; Rehab H. Taha; Keping Chen. 2021. "Complementary transcriptomic and proteomic analysis of Bombyx mori middle silk glands reveals a predominant ribosome-biogenesis regulating network during silkworm yellow-cocoon color formation." Journal of Asia-Pacific Entomology 24, no. 1: 260-270.

Research article
Published: 11 November 2020 in Journal of Proteome Research
Reads 0
Downloads 0

Lipids have been recently proposed as key molecules for virus entry and egress, and lipid biosynthesis and signaling were reported necessary for some viruses during replication and infection. The silkworm Bombyx mori is an important economic insect and a model organism, but its lipid profiles have not been systematically investigated. Most silkworm strains are susceptible to the B. mori nuclear polyhedrovirus (BmNPV), a baculovirus that causes serious loss to the sericulture industry. Previously, our lab has screened a natural mutant of B. mori that is highly resistant to BmNPV. In this study, a comprehensive lipidomic analysis by ultrahigh pressure liquid chromatography-mass spectrometry (UPLC-MS) was carried out on the BmNPV-susceptible strain 306 and resistant strain NB (data deposited in MetaboLight MTBLS2142). Comparisons of the lipid profiles between the two strains reveal that phosphosphingolipids, diacylglycerolipids, ceramides, and quinones were present at notably higher levels in the susceptible strain, while lysophosphocholines were found at a higher level in the resistant strain. BmNPV administration changed the lipid profiles in both strains, revealing key lipids involved in virus infection and immune response. Some key enzymes in the lipid biosynthesis pathway were analyzed for their activities in the two silkworm strains and their virus-administered counterparts, underlining the relation among lipid biosynthesis, viral resistance, and immune response in the host.

ACS Style

Feifei Zhu; Dandan Song; Han Chen; Qi Tang; Shuhao Huo; Xiaoyong Liu; Keping Chen. A Lipidome Map of the Silkworm Bombyx mori: Influences of Viral Infection. Journal of Proteome Research 2020, 20, 695 -703.

AMA Style

Feifei Zhu, Dandan Song, Han Chen, Qi Tang, Shuhao Huo, Xiaoyong Liu, Keping Chen. A Lipidome Map of the Silkworm Bombyx mori: Influences of Viral Infection. Journal of Proteome Research. 2020; 20 (1):695-703.

Chicago/Turabian Style

Feifei Zhu; Dandan Song; Han Chen; Qi Tang; Shuhao Huo; Xiaoyong Liu; Keping Chen. 2020. "A Lipidome Map of the Silkworm Bombyx mori: Influences of Viral Infection." Journal of Proteome Research 20, no. 1: 695-703.

Journal article
Published: 21 October 2020 in Developmental & Comparative Immunology
Reads 0
Downloads 0

Peptidoglycan recognition proteins (PGRPs) are the most important pattern recognition receptors (PRRs) in insects. PGRPs can recognize pathogenic microorganism peptidoglycans (PGs) and play an important role in innate immunity. Twelve PGRPs have been identified in silkworms. However, the specific roles played by these PGPRs in the silkworm innate immune system have not been elucidated to date. In this study, we systematically investigated the biological functions of BmPGRP-S1 in silkworms. We observed that BmPGRP-S1 was highly expressed in silkworm immune-related organs and was upregulated in response to bacterial challenges. Furthermore, we determined that BmPGRP-S1 can bind to bacteria or PGs and activate antimicrobial peptide (AMP) expression. Inhibition of the expression of BmPGRP-S1 by siRNA reduced AMP gene expression in silkworms. Further experiments demonstrated that BmPGRP-S1 is involved in IMD pathway activation to induce AMP expression. Taken together, these results demonstrate that BmPGRP-S1 serves as a receptor to activate AMP gene expression through the IMD pathway to address bacterial challenges.

ACS Style

Qiang Wang; Jiayou Wang; Meijia Ren; Shangshang Ma; Xiaoyong Liu; Keping Chen; Hengchuan Xia. Peptidoglycan recognition protein-S1 acts as a receptor to activate AMP expression through the IMD pathway in the silkworm Bombyx mori. Developmental & Comparative Immunology 2020, 115, 103903 .

AMA Style

Qiang Wang, Jiayou Wang, Meijia Ren, Shangshang Ma, Xiaoyong Liu, Keping Chen, Hengchuan Xia. Peptidoglycan recognition protein-S1 acts as a receptor to activate AMP expression through the IMD pathway in the silkworm Bombyx mori. Developmental & Comparative Immunology. 2020; 115 ():103903.

Chicago/Turabian Style

Qiang Wang; Jiayou Wang; Meijia Ren; Shangshang Ma; Xiaoyong Liu; Keping Chen; Hengchuan Xia. 2020. "Peptidoglycan recognition protein-S1 acts as a receptor to activate AMP expression through the IMD pathway in the silkworm Bombyx mori." Developmental & Comparative Immunology 115, no. : 103903.

Journal article
Published: 06 September 2020 in Journal of Asia-Pacific Entomology
Reads 0
Downloads 0

The silkworm, Bombyx mori, is an important model economic insect. Cross breeding is an important way to select excellent silkworm varieties, and different combinations among the same parents often show different genetic characters. To explore the regulatory mechanism of silk gland differences in reciprocal cross silkworm, the 3rd day of the 5th instar of the orthogonal silkworm (306♀×NB♂, 798♀×NB♂) and the reverse cross silkworm (NB♀×306♂, NB♀×798♂) silk glands were used as experimental materials, then using Label-free-based proteomics and RNA-Seq-based transcriptomics for analysis. Besides, the qRT-PCR was used to validate the expression of differentially expressed genes. The results showed that in NB♀×306♂ VS 306♀×NB♂ group, 280 differentially expressed genes and 238 differential proteins were up-regulated while 40 differentially expressed genes and 149 differential proteins were down-regulated; in NB♀×798♂ VS 798♀×NB♂, 82 differentially expressed genes and 325 differential proteins were up-regulated while 113 differentially expressed genes and 258 differential proteins were down-regulated. Further analysis indicated that the up-regulation of mitochondrial aldehyde dehydrogenase gene and cytochrome P450 gene, which are related to mitochondria, might be regulated by maternal inheritance. The GO and KEGG enrichment initially showed that the differential genes and proteins are mainly involved in processes such as protease metabolism, ATP hydrolysis, lipid transport, insect hormone synthesis, and TCA cycle. The down-regulated expression of ecdysone oxidase and Serpin-32 in the reverse cross group will reduce the energy utilization in the process of silk gland synthesis, increase the hydrolysis of serine, affect the synthesis and transport of silk protein, and cause the difference between reciprocal crosses.

ACS Style

Qi Ge; Rui Xiao; Yi Yuan; Suqun He; Liang Chen; Shangshang Ma; Rehab Hosny Taha; Qin Yao; Keping Chen. Transcriptome and proteomics-based analysis to investigate the regulatory mechanism of silk gland differences between reciprocal cross silkworm, Bombyx mori. Journal of Asia-Pacific Entomology 2020, 23, 1101 -1113.

AMA Style

Qi Ge, Rui Xiao, Yi Yuan, Suqun He, Liang Chen, Shangshang Ma, Rehab Hosny Taha, Qin Yao, Keping Chen. Transcriptome and proteomics-based analysis to investigate the regulatory mechanism of silk gland differences between reciprocal cross silkworm, Bombyx mori. Journal of Asia-Pacific Entomology. 2020; 23 (4):1101-1113.

Chicago/Turabian Style

Qi Ge; Rui Xiao; Yi Yuan; Suqun He; Liang Chen; Shangshang Ma; Rehab Hosny Taha; Qin Yao; Keping Chen. 2020. "Transcriptome and proteomics-based analysis to investigate the regulatory mechanism of silk gland differences between reciprocal cross silkworm, Bombyx mori." Journal of Asia-Pacific Entomology 23, no. 4: 1101-1113.

Journal article
Published: 14 August 2020 in Journal of Proteomics
Reads 0
Downloads 0

The application of silkworm hybrids have promoted the innovation and development of agricultural technology, but the mechanism of heterosis in silkworm has not been explained clearly. In this study, the heterosis of silkworm in the aspects of body weight, silk gland and cocoon weight was investigated by means of silkworm hybridization and multi-omics approaches, including transcriptome and proteome. The results showed that heterosis of silkworm body weight, silk gland and cocoon weight was overdominant, but only part of genes and proteins were overdominant, and most of genes and proteins were non-additive. Combined analysis obtained six up-regulated genes and four down-regulated genes that were consistent both in transcriptome and proteome. Gene functional enrichment analysis indicated that most up-regulated genes and proteins were mostly related to metabolism, which led to accelerated metabolism and protein synthesis and contributing to improved heterosis. The up-regulation of 6-phosphate glucose dehydrogenase (G6PDH), phosphatidylethanolamine-binding protein (PEBP) and sHSP20.4, which are involved in metabolism, might be related to silk gland heterosis. A combination of transcriptomic and proteomic analysis was used to understand the molecular mechanism of silkworm heterosis. We found that the phenotypic traits of silkworm are overdominant, while the analysis of transcriptome and proteome showed that only part of genes and proteins were overdominant, and most of genes and proteins were non-additive. Some of the genes had unique expression in F1, which was speculated that genes under heterozygous condition may result in rearrangement and cause metabolic changes in the hybrids. Those both up-regulated in transcriptomic and proteomic analysis were found to be involved in various metabolic processes, so as to accelerate metabolism and protein synthesis, thus exhibiting heterosis.

ACS Style

Rui Xiao; Yi Yuan; Feifei Zhu; Suqun He; Qi Ge; Xueqi Wang; Rehab Taha; Keping Chen. Transcriptomics and proteomics-based analysis of heterosis on main economic traits of silkworm, Bombyx mori. Journal of Proteomics 2020, 229, 103941 .

AMA Style

Rui Xiao, Yi Yuan, Feifei Zhu, Suqun He, Qi Ge, Xueqi Wang, Rehab Taha, Keping Chen. Transcriptomics and proteomics-based analysis of heterosis on main economic traits of silkworm, Bombyx mori. Journal of Proteomics. 2020; 229 ():103941.

Chicago/Turabian Style

Rui Xiao; Yi Yuan; Feifei Zhu; Suqun He; Qi Ge; Xueqi Wang; Rehab Taha; Keping Chen. 2020. "Transcriptomics and proteomics-based analysis of heterosis on main economic traits of silkworm, Bombyx mori." Journal of Proteomics 229, no. : 103941.

Article
Published: 06 July 2020 in Molecular and Cellular Biochemistry
Reads 0
Downloads 0

Food additives are widely used in various food products to preserve the taste, color, and other qualities. However, if they are used improperly or exceed the standard, they will cause damage to the human body. Sulfite is a commonly used food additive to prevent oxidation from deteriorating the nutrients in foods, it has been widely used as a bleaching agent in the food industry for a long time. In this study, human hepatocytes L02 cells were used as a model cell line to evaluate the toxicity of sodium sulfite. The cell morphology and cell proliferation were affected by sodium sulfite treatment, and apoptosis was detected. Transcriptome sequencing showed 97 differentially expressed genes (DEGs) between the experimental group (IC50) and the control group (MOCK), and 27 differentially expressed genes related to cell apoptosis, metabolism and inflammation were selected for validation by qPCR. Among them, 13 significantly upregulated genes and 14 significantly downregulated genes were identified by qPCR. The results showed that with increase of sodium sulfite concentration, the morphology of L02 changed, cell proliferation and activity were inhibited, and sodium sulfite caused apoptosis in a concentration- and time-dependent manner. The resulting toxic mechanism inhibits proliferation, damages the mitochondrial integrity, and promotes apoptosis.

ACS Style

Xu Han; Feifei Zhu; Liang Chen; Hong Wu; Tao Wang; Keping Chen. Mechanism analysis of toxicity of sodium sulfite to human hepatocytes L02. Molecular and Cellular Biochemistry 2020, 473, 25 -37.

AMA Style

Xu Han, Feifei Zhu, Liang Chen, Hong Wu, Tao Wang, Keping Chen. Mechanism analysis of toxicity of sodium sulfite to human hepatocytes L02. Molecular and Cellular Biochemistry. 2020; 473 (1-2):25-37.

Chicago/Turabian Style

Xu Han; Feifei Zhu; Liang Chen; Hong Wu; Tao Wang; Keping Chen. 2020. "Mechanism analysis of toxicity of sodium sulfite to human hepatocytes L02." Molecular and Cellular Biochemistry 473, no. 1-2: 25-37.

Journal article
Published: 11 June 2020 in Journal of Invertebrate Pathology
Reads 0
Downloads 0

The resistance of silkworm to infection by Bombyx mori nuclear polyhedrosis virus (BmNPV) is a main focus of sericultural research. Previously, a BmNPV-resistant strain, NB, was identified among a collection of Chinese silkworm strains in our lab. To better understand the molecular mechanism of NB strain resistance, the patterns of host immune response gene transcription in resistant (NB) and susceptible (306) strains were examined. Quantative real-time PCR (qRT-PCR) revealed that multiple insect innate immune signaling pathways (Toll, Imd and JAK/STAT) were strongly activated upon infection with BmNPV. Notably, Suppressor of cytokine signaling 2 (BmSOCS2) mRNA expression was significantly up-regulated in midgut tissues of the resistant NB strain, suggesting that the BmSOCS2 gene product may be involved in host immune defense against BmNPV infection. A significant inhibition of BmNPV replication was also observed in BmN cells transfected with a vector encoding BmSOCS2. The results suggest that BmSOCS2 is a key gene involved in the resistance of the NB silkworm strain to BmNPV infection.

ACS Style

Yi Yuan; Feifei Zhu; Rui Xiao; Qi Ge; Hanhan Tang; Ming Kong; Rehab H. Taha; Keping Chen. Increased expression of Suppressor of cytokine signaling 2 (BmSOCS2) is correlated with suppression of Bombyx mori nucleopolyhedrovirus replication in silkworm larval tissues and cells. Journal of Invertebrate Pathology 2020, 174, 107419 .

AMA Style

Yi Yuan, Feifei Zhu, Rui Xiao, Qi Ge, Hanhan Tang, Ming Kong, Rehab H. Taha, Keping Chen. Increased expression of Suppressor of cytokine signaling 2 (BmSOCS2) is correlated with suppression of Bombyx mori nucleopolyhedrovirus replication in silkworm larval tissues and cells. Journal of Invertebrate Pathology. 2020; 174 ():107419.

Chicago/Turabian Style

Yi Yuan; Feifei Zhu; Rui Xiao; Qi Ge; Hanhan Tang; Ming Kong; Rehab H. Taha; Keping Chen. 2020. "Increased expression of Suppressor of cytokine signaling 2 (BmSOCS2) is correlated with suppression of Bombyx mori nucleopolyhedrovirus replication in silkworm larval tissues and cells." Journal of Invertebrate Pathology 174, no. : 107419.

Original article
Published: 30 May 2020 in Journal of Applied Microbiology
Reads 0
Downloads 0

Aims To develop an oral delivery system of glucagon‐like peptide 1 (GLP‐1)(28‐36) for treating Type‐2 Diabetes, B.S‐GLP‐1(28‐36), a recombinant Bacillus subtilis spores transformed with a plasmid vector encoding five consecutive GLP‐1 (28‐36) nonapeptides with an enterokinase site was constructed. Methods and Results GLP‐1(28‐36) nonapeptide was successfully expressed on the surface of B. subtilis spores and validated by western blot and immunofluorescence. The therapeutic effect of oral administration of B.S‐GLP‐1 (28‐36) spores was evaluated in type 2 diabetic model mice. The efficacy of recombinant spores was examined for a period of 13 weeks after oral administration in diabetic mice. At the end of the sixth week, diabetic mice with oral administration of BS‐GLP‐1 (28‐36) spores showed decreased blood glucose levels from 2.4×102 mol l‐1 to 1.7×102 mol l‐1. By the ninth week, the mean fasting blood glucose level in the experimental group was significantly lower than that in the control group 30 min after injection of pyruvate. At the end of the tenth week of oral administration, the blood glucose of the experimental group was significantly lower than that of the control group after intraperitoneal injection of glucose. By the twelfth week, fasting blood glucose level and fasting insulin level were measured in all mice, the results showed that the recombinant spores increased the insulin sensitivity of mice. Conclusions The results of pathological observation showed that the recombinant spores also had a certain protective effect on the liver and islets of mice, and the content of GLP‐1 (28‐36) in the pancreas of the experimental group was increased. Significance and Impact of Study The results of this study revealed that GLP‐1 (28‐36) nonapeptides can reduce blood glucose and play an important role in the treatment of type 2 diabetes.

ACS Style

Mengna Kang; Fan Feng; Q. Ge; Feifei Zhu; Liang Chen; Peng Lv; Shangshang Ma; Qin Yao; Keping Chen. Display of quintuple glucagon‐like peptide 1 (28–36) nonapeptide on Bacillus subtilis spore for oral administration in the treatment of type 2 diabetes. Journal of Applied Microbiology 2020, 130, 314 -324.

AMA Style

Mengna Kang, Fan Feng, Q. Ge, Feifei Zhu, Liang Chen, Peng Lv, Shangshang Ma, Qin Yao, Keping Chen. Display of quintuple glucagon‐like peptide 1 (28–36) nonapeptide on Bacillus subtilis spore for oral administration in the treatment of type 2 diabetes. Journal of Applied Microbiology. 2020; 130 (2):314-324.

Chicago/Turabian Style

Mengna Kang; Fan Feng; Q. Ge; Feifei Zhu; Liang Chen; Peng Lv; Shangshang Ma; Qin Yao; Keping Chen. 2020. "Display of quintuple glucagon‐like peptide 1 (28–36) nonapeptide on Bacillus subtilis spore for oral administration in the treatment of type 2 diabetes." Journal of Applied Microbiology 130, no. 2: 314-324.

Journal article
Published: 29 April 2020 in Journal of Proteomics
Reads 0
Downloads 0

The biological functions of protein glycosylation have been increasingly recognized but not yet been very well understood, especially in lower organisms. Silkworm as a model lepidopteran insect and important economic insect, has been widely studied in life science, however, the current knowledge on the glycosylation status of its proteome is not satisfactory, and little is known about how pathogenic infections could affect the glycosylation status. This study performed large scale glycosite mapping for the silkworm Bombyx mori P50 strain, and quantitatively compared with that infected with the Bombyx mori cytoplasmic polyhedrosis virus (BmCPV). Some 400 glycoproteins were mapped in the silkworm, including N- and O-glycoproteins. Upon virus infection, the glycosylation levels of 41 N-glycopeptides were significantly changed, some of them belonging to transmembrane glycoproteins. The O-glycosylation profiles were also affected. In addition, 4 BmCPV-encoded viral proteins were found to be glycosylated for the first time, including polyhedrin, P101, VP3, and the NS protein. This study drafted a silkworm protein glycosylation map and underlined the potential impact of virus infection on glycosylation. This study reveals the characteristics of the glycoproteome in the silkworm strain P50, and quantitatively compared to that infected by the virus BmCPV, which underlines the impact of virus infection on the alteration of protein glycosylation in invertebrate species. Our findings add to the knowledge of the post translational modifications of this model organism, and also uncovered for the first time the glycosylation status of the viral proteins expressed by BmCPV.

ACS Style

Feifei Zhu; Dong Li; Dandan Song; Shuhao Huo; Shangshang Ma; Peng Lü; Xiaoyong Liu; Qin Yao; Keping Chen. Glycoproteome in silkworm Bombyx mori and alteration by BmCPV infection. Journal of Proteomics 2020, 222, 103802 .

AMA Style

Feifei Zhu, Dong Li, Dandan Song, Shuhao Huo, Shangshang Ma, Peng Lü, Xiaoyong Liu, Qin Yao, Keping Chen. Glycoproteome in silkworm Bombyx mori and alteration by BmCPV infection. Journal of Proteomics. 2020; 222 ():103802.

Chicago/Turabian Style

Feifei Zhu; Dong Li; Dandan Song; Shuhao Huo; Shangshang Ma; Peng Lü; Xiaoyong Liu; Qin Yao; Keping Chen. 2020. "Glycoproteome in silkworm Bombyx mori and alteration by BmCPV infection." Journal of Proteomics 222, no. : 103802.

Journal article
Published: 11 April 2020 in Fish & Shellfish Immunology
Reads 0
Downloads 0

Snakehead vesiculovirus (SHVV) causes enormous economic losses in snakehead fish (Ophicephalus striatus) culture. Understanding replication mechanisms of virus is considerable significance in preventing and treating viral disease. In our previous studies, we have reported that glutamine starvation could significant inhibit the replication of SHVV. Furthermore, we also showed that SHVV infection could cause apoptosis of striped snakehead fish cells (SSN-1). However, the underlying mechanisms remain enigmatic. To decipher the relationships among the viral infection, glutamine starvation and apoptosis, SSN-1 cells transcriptomic profilings of SSN-1 cells infected with or without SHVV under glutamine deprived condition were analyzed. RNA-seq was used to identify differentially expressed genes (DEGs). Our data revealed that 1215 up-regulated and 226 down-regulated genes at 24 h post-infection were involved in MAPK, apoptosis, RIG-1-like and toll-like receptors pathways and glutamine metabolism. Subsequently, DEGs of glutamine metabolism and apoptosis pathways were selected to validate the sequencing data by quantitative real-time PCR (qRT-PCR). The expression patterns of both transcriptomic data and qRT-PCR were consistent. We observed that lack of glutamine alone could cause mild cellular apoptosis. However, lack of glutamine together with SHVV infection could synergistically enhance cellular apoptosis. When the cells were cultured in complete medium with glutamine, overexpression of glutaminase (GLS), an essential enzyme for glutamine metabolism, could significantly enhance the SHVV replication. While, SHVV replication was decreased in cells when GLS was knocked down by specific siRNA, indicating that glutamine metabolism was essential for viral replication. Furthermore, the expression level of caspase-3 and Bax was significantly decreased in SHVV infected cells with GLS overexpression. By contrast, they were significantly increased in SHVV infected cells with GLS silence by SiRNA, indicating that SHVV infection activated the Bax and caspase-3 pathways to induce apoptosis independent of glutamine. Our results reveal that SHVV replication and starvation of glutamine could synergistically promote the cellular apoptosis, which will pave a new way for developing strategies against the vial infection.

ACS Style

Lindan Sun; V Sarath Babu; Zhendong Qin; Youlu Su; Chun Liu; Fei Shi; Lijuan Zhao; Jun Li; Keping Chen; Li Lin; Sarath Babu V. Snakehead vesiculovirus (SHVV) infection alters striped snakehead (Ophicephalus striatus) cells (SSN-1) glutamine metabolism and apoptosis pathways. Fish & Shellfish Immunology 2020, 102, 36 -46.

AMA Style

Lindan Sun, V Sarath Babu, Zhendong Qin, Youlu Su, Chun Liu, Fei Shi, Lijuan Zhao, Jun Li, Keping Chen, Li Lin, Sarath Babu V. Snakehead vesiculovirus (SHVV) infection alters striped snakehead (Ophicephalus striatus) cells (SSN-1) glutamine metabolism and apoptosis pathways. Fish & Shellfish Immunology. 2020; 102 ():36-46.

Chicago/Turabian Style

Lindan Sun; V Sarath Babu; Zhendong Qin; Youlu Su; Chun Liu; Fei Shi; Lijuan Zhao; Jun Li; Keping Chen; Li Lin; Sarath Babu V. 2020. "Snakehead vesiculovirus (SHVV) infection alters striped snakehead (Ophicephalus striatus) cells (SSN-1) glutamine metabolism and apoptosis pathways." Fish & Shellfish Immunology 102, no. : 36-46.

Original article
Published: 31 January 2020 in Molecular Genetics and Genomics
Reads 0
Downloads 0

Achaete-scute complex (ASC) genes play essential roles in regulating neurogenesis of metazoans. Various metazoan species have greatly different numbers of genes in ASCa, ASCb and ASCc families. To explore evolutionary mechanisms of metazoan ASC genes, Blast (basic local alignment search tool) searches and phylogenetic analyses were conducted to identify ASC genes in metazoan species and to infer phylogenetic relationship between various ASC genes. As a result, 2784 ASC genes were identified in 804 metazoan species. The phylogenetic tree constructed using 1237 unique bHLH motifs shows that metazoan ASCa, ASCb and ASCc families contain six (a1-a6), five (b1-b5) and three (c1-c3) bHLH genes, respectively. Further phylogenetic analyses suggest that ASC genes in metazoans are derived from a primitive c gene, those in insects are derived from c2 gene, and those in chordates are derived from a2 and a3 genes. Data of gene linkage demonstrate that insect a6 is derived from a4 but not from a5, and chordate a2 is ancestral to b5 only, whilst a3 is ancestral to both b3 and b5. It is concluded that current ASC gene families in metazoans were established through a series of sub- and/or neo-functionalization to duplicated ancestral ASC gene(s). These results provide good references for exploring evolutionary mechanisms of other bHLH genes in metazoans. Besides, gene subtyping is considered as an efficient method for evolutionary studies on closely related homologous genes.

ACS Style

Yong Wang; Guang-Dong Wang; Qin-Liu He; Zhi-Peng Luo; Liu Yang; Qin Yao; Ke-Ping Chen. Phylogenetic analysis of achaete–scute complex genes in metazoans. Molecular Genetics and Genomics 2020, 295, 591 -606.

AMA Style

Yong Wang, Guang-Dong Wang, Qin-Liu He, Zhi-Peng Luo, Liu Yang, Qin Yao, Ke-Ping Chen. Phylogenetic analysis of achaete–scute complex genes in metazoans. Molecular Genetics and Genomics. 2020; 295 (3):591-606.

Chicago/Turabian Style

Yong Wang; Guang-Dong Wang; Qin-Liu He; Zhi-Peng Luo; Liu Yang; Qin Yao; Ke-Ping Chen. 2020. "Phylogenetic analysis of achaete–scute complex genes in metazoans." Molecular Genetics and Genomics 295, no. 3: 591-606.

Journal article
Published: 02 January 2020 in Pesticide Biochemistry and Physiology
Reads 0
Downloads 0

Baculoviruses have been developed as long-term and environmentally friendly biopesticides. However, solar ultraviolet radiation can reduce the activity of baculovirus. Radiation sensitive 23 (Rad23) can recognize DNA damage and is involved with nucleotide excision repair (NER). In the current study, BmRad23 was accumulated mainly within the nucleus. Host cell reactivation (HCR) assays have shown that BmRad23 significantly facilitated the expression of UV-damaged mCherry reporter gene. Reverse transcription quantitative PCR (RT-qPCR) result showed that the mRNA expression level of BmRad23 was increased in (Bombyx mori nuclear polyhedrovirus, BmNPV) BmNPV-infected BmN cells. However, the expression of BmRad23 was increased significantly when BmNPV budded viruses (BVs) or BmN cells were irradiated with UV light. Overexpression of BmRad23 promoted the mRNA levels of two UV-induced DNA damage repair genes which were from Bombyx mori and BmNPV, respectively. Meanwhile, the overexpression of BmRad23 in BmN cells was conducive to the proliferation of BmNPV and UV-damaged BmNPV. The recombinant BmNPV BVs expressing BmRad23 showed stronger resistance to UV radiation than the control virus. In conclusion, the results revealed that BmRad23 contributed to the proliferation of BmNPV and the repair of UV-damaged BmNPV, which would provide a reference for the development of efficient baculovirus pesticides against UV radiation.

ACS Style

Qi Tang; Fangying Chen; Xinyu Qi; Peng Wu; Huiqin Chen; Lipeng Qiu; Zhaoyang Hu; Keping Chen; Guohui Li. Bombyx mori Rad23 (BmRad23) contributes to the repair of UV-damaged BmNPV. Pesticide Biochemistry and Physiology 2020, 164, 91 -99.

AMA Style

Qi Tang, Fangying Chen, Xinyu Qi, Peng Wu, Huiqin Chen, Lipeng Qiu, Zhaoyang Hu, Keping Chen, Guohui Li. Bombyx mori Rad23 (BmRad23) contributes to the repair of UV-damaged BmNPV. Pesticide Biochemistry and Physiology. 2020; 164 ():91-99.

Chicago/Turabian Style

Qi Tang; Fangying Chen; Xinyu Qi; Peng Wu; Huiqin Chen; Lipeng Qiu; Zhaoyang Hu; Keping Chen; Guohui Li. 2020. "Bombyx mori Rad23 (BmRad23) contributes to the repair of UV-damaged BmNPV." Pesticide Biochemistry and Physiology 164, no. : 91-99.