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Organic UV filters in sunscreen products are released to aquatic ecosystems through human recreational activities and urban wastewater treatment plant effluents. The biodegradation of three organic UV filters, 2-ethylhexyl salicylate (EHS), homosalate (HMS) and ethylhexyl methoxycinnamate (EHMC), which cannot be effectively removed by conventional wastewater treatment plants, was investigated in this study. Spent mushroom compost (SMC), a waste product of the mushroom industry, which contains white-rot fungus extracellular enzymes, was tested for its ability to remove the three organic UV filters. The results of batch experiments revealed that the SMC enzyme extract of Pleurotus djamor exhibited the highest ability for EHS and HMS removal. The results of bioreactor experiments indicated that direct application of SMCs may be a feasible solution to remove EHS and HMS from urban wastewater. The application of SMCs for the removal of organic UV filters can be developed into a green and sustainable technology.
Chu-Wen Yang; Ping-Hsun Tu; Wen-Yi Tso; Bea-Ven Chang. Removal of Organic UV Filters Using Enzymes in Spent Mushroom Composts from Fungicultures. Applied Sciences 2021, 11, 3932 .
AMA StyleChu-Wen Yang, Ping-Hsun Tu, Wen-Yi Tso, Bea-Ven Chang. Removal of Organic UV Filters Using Enzymes in Spent Mushroom Composts from Fungicultures. Applied Sciences. 2021; 11 (9):3932.
Chicago/Turabian StyleChu-Wen Yang; Ping-Hsun Tu; Wen-Yi Tso; Bea-Ven Chang. 2021. "Removal of Organic UV Filters Using Enzymes in Spent Mushroom Composts from Fungicultures." Applied Sciences 11, no. 9: 3932.
Background The innate immune system especially Toll-like receptor (TLR) 7/8 and the interferon pathway, constitutes an important first line of defense against single-stranded RNA viruses. However, large-scale, systematic comparisons of the TLR 7/8-stimulating potential of genomic RNAs of single-stranded RNA viruses are rare. In this study, a computational method to evaluate the human TLR 7/8-stimulating ability of single-stranded RNA virus genomes based on their human TLR 7/8-stimulating trimer compositions was used to analyze 1,002 human coronavirus genomes. Results The human TLR 7/8-stimulating potential of coronavirus genomic (positive strand) RNAs followed the order of NL63-CoV > HKU1-CoV >229E-CoV ≅ OC63-CoV > SARS-CoV-2 > MERS-CoV > SARS-CoV. These results suggest that among these coronaviruses, MERS-CoV, SARS-CoV and SARS-CoV-2 may have a higher ability to evade the human TLR 7/8-mediated innate immune response. Analysis with a logistic regression equation derived from human coronavirus data revealed that most of the 1,762 coronavirus genomic (positive strand) RNAs isolated from bats, camels, cats, civets, dogs and birds exhibited weak human TLR 7/8-stimulating potential equivalent to that of the MERS-CoV, SARS-CoV and SARS-CoV-2 genomic RNAs. Conclusions Prediction of the human TLR 7/8-stimulating potential of viral genomic RNAs may be useful for surveillance of emerging coronaviruses from nonhuman mammalian hosts.
Chu-Wen Yang; Mei-Fang Chen. Low compositions of human toll-like receptor 7/8-stimulating RNA motifs in the MERS-CoV, SARS-CoV and SARS-CoV-2 genomes imply a substantial ability to evade human innate immunity. PeerJ 2021, 9, e11008 .
AMA StyleChu-Wen Yang, Mei-Fang Chen. Low compositions of human toll-like receptor 7/8-stimulating RNA motifs in the MERS-CoV, SARS-CoV and SARS-CoV-2 genomes imply a substantial ability to evade human innate immunity. PeerJ. 2021; 9 ():e11008.
Chicago/Turabian StyleChu-Wen Yang; Mei-Fang Chen. 2021. "Low compositions of human toll-like receptor 7/8-stimulating RNA motifs in the MERS-CoV, SARS-CoV and SARS-CoV-2 genomes imply a substantial ability to evade human innate immunity." PeerJ 9, no. : e11008.
Intensive fish farming through aquaculture is vulnerable to infectious diseases that can increase fish mortality and damage the productivity of aquaculture farms. To prevent infectious diseases, malachite green (MG) has been applied as a veterinary drug for various microbial infections in aquaculture settings worldwide. However, little is known regarding the consequences of MG and MG-degrading bacteria (MGDB) on microbial communities in milkfish culture ponds (MCPs). In this study, small MCPs were used as a model system to determine the effects of MG on the microbial communities in MCPs. The addition of MG led to cyanobacterial blooms in the small MCP. The addition of MGDB could not completely reverse the effects of MG on microbial communities. Cyanobacterial blooms were not prevented. Microbial communities analyzed by next generation sequencing revealed that cyanobacterial blooms may be due to increase of nitrogen cycle (including nitrogen fixation, nitrate reduction and anammox) associated microbial communities, which raised the levels of ammonium in the water of the small MCP. The communities of anoxygenic phototrophic bacteria (beneficial for aquaculture and aquatic ecosystems) decreased after the addition of MG. The results of this investigation provide valuable insights into the effects of MG in aquaculture and the difficulties of bioremediation for aquatic environments polluted by MG.
Chu-Wen Yang; Yi-Tang Chang; Chi-Yen Hsieh; Bea-Ven Chang. Effects of Malachite Green on the Microbiomes of Milkfish Culture Ponds. Water 2021, 13, 411 .
AMA StyleChu-Wen Yang, Yi-Tang Chang, Chi-Yen Hsieh, Bea-Ven Chang. Effects of Malachite Green on the Microbiomes of Milkfish Culture Ponds. Water. 2021; 13 (4):411.
Chicago/Turabian StyleChu-Wen Yang; Yi-Tang Chang; Chi-Yen Hsieh; Bea-Ven Chang. 2021. "Effects of Malachite Green on the Microbiomes of Milkfish Culture Ponds." Water 13, no. 4: 411.
A coronavirus pandemic caused by a novel coronavirus (SARS-CoV-2) has spread rapidly worldwide since December 2019. Improved understanding and new strategies to cope with novel coronaviruses are urgently needed. Viruses (especially RNA viruses) encode a limited number and size (length of polypeptide chain) of viral proteins and must interact with the host cell components to control (hijack) the host cell machinery. To achieve this goal, the extensive mimicry of SLiMs in host proteins provides an effective strategy. However, little is known regarding SLiMs in coronavirus proteins and their potential targets in host cells. The objective of this study is to uncover SLiMs in coronavirus proteins that are present within host cells. These SLiMs have a high possibility of interacting with host intracellular proteins and hijacking the host cell machinery for virus replication and dissemination. In total, 1,479 SLiM hits were identified in the 16 proteins of 590 coronaviruses infecting humans. Overall, 106 host proteins were identified that may interact with SLiMs in 16 coronavirus proteins. These SLiM-interacting proteins are composed of many intracellular key regulators, such as receptors, transcription factors and kinases, and may have important contributions to virus replication, immune evasion and viral pathogenesis. A total of 209 pathways containing proteins that may interact with SLiMs in coronavirus proteins were identified. This study uncovers potential mechanisms by which coronaviruses hijack the host cell machinery. These results provide potential therapeutic targets for viral infections.
Chu-Wen Yang; Zhi-Ling Shi. Uncovering potential host proteins and pathways that may interact with eukaryotic short linear motifs in viral proteins of MERS, SARS and SARS2 coronaviruses that infect humans. PLOS ONE 2021, 16, e0246150 .
AMA StyleChu-Wen Yang, Zhi-Ling Shi. Uncovering potential host proteins and pathways that may interact with eukaryotic short linear motifs in viral proteins of MERS, SARS and SARS2 coronaviruses that infect humans. PLOS ONE. 2021; 16 (2):e0246150.
Chicago/Turabian StyleChu-Wen Yang; Zhi-Ling Shi. 2021. "Uncovering potential host proteins and pathways that may interact with eukaryotic short linear motifs in viral proteins of MERS, SARS and SARS2 coronaviruses that infect humans." PLOS ONE 16, no. 2: e0246150.
The removal of antibiotics from the aquatic environment has received great interest. The aim of this study is to examine degradation of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), amoxicillin (AMO), sulfamethazine (SMZ), sulfamethoxazole (SMX), sulfadimethoxine (SDM) in sludge. Four antibiotic-degrading bacterial strains, SF1 (Pseudmonas sp.), A12 (Pseudmonas sp.), strains B (Bacillus sp.), and SANA (Clostridium sp.), were isolated, identified and tested under aerobic and anaerobic conditions in this study. Batch experiments indicated that the addition of SF1 and A12 under aerobic conditions and the addition of B and SANA under anaerobic conditions increased the biodegradation of antibiotics in sludge. Moreover, the results of repeated addition experiments indicated that the efficiency of the biodegradation of antibiotics using the isolated bacterial strains could be maintained for three degradation cycles. Two groups of potential microbial communities associated with the aerobic and anaerobic degradation of SMX, AMO and CTC in sludge were revealed. Twenty-four reported antibiotics-degrading bacterial genera (Achromobacter, Acidovorax, Acinetobacter, Alcaligenes, Bacillus, Burkholderia, Castellaniella, Comamonas, Corynebacterium, Cupriavidus, Dechloromonas, Geobacter, Gordonia, Klebsiella, Mycobacterium, Novosphingobium, Pandoraea, Pseudomonas, Rhodococcus, Sphingomonas, Thauera, Treponema, Vibrio and Xanthobacter) were found in both the aerobic and anaerobic groups, suggesting that these 24 bacterial genera may be the major antibiotic-degrading bacteria in sludge.
Chu-Wen Yang; Chien Liu; Bea-Ven Chang. Biodegradation of Amoxicillin, Tetracyclines and Sulfonamides in Wastewater Sludge. Water 2020, 12, 2147 .
AMA StyleChu-Wen Yang, Chien Liu, Bea-Ven Chang. Biodegradation of Amoxicillin, Tetracyclines and Sulfonamides in Wastewater Sludge. Water. 2020; 12 (8):2147.
Chicago/Turabian StyleChu-Wen Yang; Chien Liu; Bea-Ven Chang. 2020. "Biodegradation of Amoxicillin, Tetracyclines and Sulfonamides in Wastewater Sludge." Water 12, no. 8: 2147.
Acetaminophen (ACE) is a widely used medicine. Currently, concerns regarding its potential adverse effects on the environments are raised. The aim of this study was to evaluate ACE biodegradation in mangrove sediments under aerobic and anaerobic conditions. Three ACE biodegradation strategies in mangrove sediments were tested. The degradation half-lives (t1/2) of ACE in the sediments with spent mushroom compost under aerobic conditions ranged from 3.24 ± 0.16 to 6.25 ± 0.31 d. The degradation half-lives (t1/2) of ACE in sediments with isolated bacterial strains ranged from 2.54 ± 0.13 to 3.30 ± 0.17 d and from 2.62 ± 0.13 to 3.52 ± 0.17 d under aerobic and anaerobic conditions, respectively. The degradation half-lives (t1/2) of ACE in sediments amended with NaNO3, Na2SO4 and NaHCO3 under anaerobic conditions ranged from 1.16 ± 0.06 to 3.05 ± 0.15 d, 2.39 ± 0.12 to 3.84 ± 0.19 d and 2.79 ± 0.14 to 10.75 ± 0.53 d, respectively. The addition of the three electron acceptors enhanced ACE degradation in mangrove sediments, where NaNO3 yielded the best effects. Sixteen microbial genera were identified as the major members of microbial communities associated in anaerobic ACE degradation in mangrove sediments with addition of NaNO3 and Na2SO4. Three (Arthrobacter, Enterobacter and Bacillus) of the sixteen microbial genera were identified in the isolated ACE-degrading bacterial strains.
Chu-Wen Yang; Yi-En Chen; Bea-Ven Chang. Microbial Communities Associated with Acetaminophen Biodegradation from Mangrove Sediment. Sustainability 2020, 12, 5410 .
AMA StyleChu-Wen Yang, Yi-En Chen, Bea-Ven Chang. Microbial Communities Associated with Acetaminophen Biodegradation from Mangrove Sediment. Sustainability. 2020; 12 (13):5410.
Chicago/Turabian StyleChu-Wen Yang; Yi-En Chen; Bea-Ven Chang. 2020. "Microbial Communities Associated with Acetaminophen Biodegradation from Mangrove Sediment." Sustainability 12, no. 13: 5410.
Translation of a genetic codon without a cognate tRNA gene is affected by both the cognate tRNA availability and the interaction with non-cognate isoacceptor tRNAs. Moreover, two consecutive slow codons (slow di-codons) lead to a much slower translation rate. Calculating the composition of host specific slow codons and slow di-codons in the viral protein coding sequences can predict the order of viral protein synthesis rates between different virus strains. Comparison of human-specific slow codon and slow di-codon compositions in the genomes of 590 coronaviruses infect humans revealed that the protein synthetic rates of 2019 novel coronavirus (2019-nCoV) and severe acute respiratory syndrome-related coronavirus (SARS-CoV) may be much faster than other coronaviruses infect humans. Analysis of host-specific slow codon and di-codon compositions provides links between viral genomic sequences and capability of virus replication in host cells that may be useful for surveillance of the transmission potential of novel viruses.
Chu-Wen Yang; Mei-Fang Chen. Composition of human-specific slow codons and slow di-codons in SARS-CoV and 2019-nCoV are lower than other coronaviruses suggesting a faster protein synthesis rate of SARS-CoV and 2019-nCoV. Journal of Microbiology, Immunology and Infection 2020, 53, 419 -424.
AMA StyleChu-Wen Yang, Mei-Fang Chen. Composition of human-specific slow codons and slow di-codons in SARS-CoV and 2019-nCoV are lower than other coronaviruses suggesting a faster protein synthesis rate of SARS-CoV and 2019-nCoV. Journal of Microbiology, Immunology and Infection. 2020; 53 (3):419-424.
Chicago/Turabian StyleChu-Wen Yang; Mei-Fang Chen. 2020. "Composition of human-specific slow codons and slow di-codons in SARS-CoV and 2019-nCoV are lower than other coronaviruses suggesting a faster protein synthesis rate of SARS-CoV and 2019-nCoV." Journal of Microbiology, Immunology and Infection 53, no. 3: 419-424.
To cope with bacterial infections, broad-spectrum antibiotics such as sulfonamides have been largely used for intensive coastal aquaculture. Sulfonamides are stable and difficult to remove by conventional wastewater treatment. Environmental pollution will occur if sulfonamide-containing aquaculture wastewater is discharged into rivers and oceans. In this study, high salinity-tolerant bacterial strains A12 and L with sulfamethoxazole (SMX)-degrading ability from milkfish (Chanos chanos) culture pond sediments with SMX were isolated, identified, and characterized. The degradation of SMX and the changes in the bacterial community in milkfish culture pond sediments were assessed. Phylogenetic analysis using 16S rRNA gene sequences suggested that bacterial strain A12 was very close (99% sequence identity) to Vibrio sp., and bacterial strain L was very close (99% sequence identity) to Pseudomonas sp. Aerobic and anaerobic batch and continuous SMX addition experiments indicated that bacterial strains A12 and L could enhance SMX degradation in milkfish culture pond sediments. Different microbial community compositions under aerobic and anaerobic conditions exhibited different SMX-degrading abilities. The results of this study suggest that bacterial strains A12 and L provide a solution for treatment of wastewater and sediment from SMX-contaminated high salinity milkfish culture ponds.
Bea-Ven Chang; Wei-Liang Chao; Shinn-Lih Yeh; Dong-Lin Kuo; Chu-Wen Yang. Biodegradation of Sulfamethoxazole in Milkfish (Chanos chanos) Pond Sediments. Applied Sciences 2019, 9, 4000 .
AMA StyleBea-Ven Chang, Wei-Liang Chao, Shinn-Lih Yeh, Dong-Lin Kuo, Chu-Wen Yang. Biodegradation of Sulfamethoxazole in Milkfish (Chanos chanos) Pond Sediments. Applied Sciences. 2019; 9 (19):4000.
Chicago/Turabian StyleBea-Ven Chang; Wei-Liang Chao; Shinn-Lih Yeh; Dong-Lin Kuo; Chu-Wen Yang. 2019. "Biodegradation of Sulfamethoxazole in Milkfish (Chanos chanos) Pond Sediments." Applied Sciences 9, no. 19: 4000.
Intensive farming practices are typically used for aquaculture. To prevent disease outbreaks, antibiotics are often used to reduce pathogenic bacteria in aquaculture animals. However, the effects of antibiotics on water quality and microbial communities in euryhaline fish culture ponds are largely unknown. The aim of this study was to investigate the interactions between sulfamethoxazole (SMX), water quality and microbial communities in milkfish (Chanos chanos) culture ponds. The results of small-scale milkfish pond experiments indicated that the addition of SMX decreased the abundance of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and photosynthetic bacteria. Consequently, the levels of ammonia and total phosphorus in the fish pond water increased, causing algal and cyanobacterial blooms to occur. In contrast, the addition of the SMX-degrading bacterial strains A12 and L effectively degraded SMX and reduced the levels of ammonia and total phosphorus in fish pond water. Furthermore, the abundances of AOB, NOB and photosynthetic bacteria were restored, and algal and cyanobacterial blooms were inhibited. This study demonstrate the influences of SMX on water quality and microbial community composition in milkfish culture ponds. Moreover, the use of the bacterial strains A12 and L as dual function (bioaugmentation and water quality maintenance) beneficial bacteria was shown to provide an effective approach for the bioremediation of SMX-contaminated euryhaline milkfish culture ponds.
Bea-Ven Chang; Yi-Tang Chang; Wei-Liang Chao; Shinn-Lih Yeh; Dong-Lin Kuo; Chu-Wen Yang. Effects of sulfamethoxazole and sulfamethoxazole-degrading bacteria on water quality and microbial communities in milkfish ponds. Environmental Pollution 2019, 252, 305 -316.
AMA StyleBea-Ven Chang, Yi-Tang Chang, Wei-Liang Chao, Shinn-Lih Yeh, Dong-Lin Kuo, Chu-Wen Yang. Effects of sulfamethoxazole and sulfamethoxazole-degrading bacteria on water quality and microbial communities in milkfish ponds. Environmental Pollution. 2019; 252 ():305-316.
Chicago/Turabian StyleBea-Ven Chang; Yi-Tang Chang; Wei-Liang Chao; Shinn-Lih Yeh; Dong-Lin Kuo; Chu-Wen Yang. 2019. "Effects of sulfamethoxazole and sulfamethoxazole-degrading bacteria on water quality and microbial communities in milkfish ponds." Environmental Pollution 252, no. : 305-316.
Malachite green (MG) is usually applied as a biocide in aquaculture worldwide. The microbial degradation of MG and changes in the microbial community composition of milkfish (Chanos chanos) culture pond sediments were assessed in this study. Three MG-degrading bacteria strains—M6, M10, and M12—were isolated, identified, and characterized. Strains M6, M10, and M12 are closely related to Zhouia amylolytica, Tenacibaculum mesophilum, and Enterobacter cloacae, respectively. The bacterial strains M10 and M12 showed good ability to degrade MG in the sediment. The MG degradation rate was increased after adding MG three more times. The microbial community in the sediment changes with different treatments. The bacterial strains M10 and M12 provide a potential solution for the treatment of sediment of saline aquaculture ponds with MG contamination.
Chu-Wen Yang; Wei-Liang Chao; Chi-Yen Hsieh; Bea-Ven Chang. Biodegradation of Malachite Green in Milkfish Pond Sediments. Sustainability 2019, 11, 4179 .
AMA StyleChu-Wen Yang, Wei-Liang Chao, Chi-Yen Hsieh, Bea-Ven Chang. Biodegradation of Malachite Green in Milkfish Pond Sediments. Sustainability. 2019; 11 (15):4179.
Chicago/Turabian StyleChu-Wen Yang; Wei-Liang Chao; Chi-Yen Hsieh; Bea-Ven Chang. 2019. "Biodegradation of Malachite Green in Milkfish Pond Sediments." Sustainability 11, no. 15: 4179.
Globally, coastal aquaculture is growing due to the large demand for marine products. Specific impacts caused by coastal aquaculture on the environment include the discharge of culture farm effluents, stress on ground water (the absence of recycling), nutrient pollution, and diseases of cultured animals. Three methods, integrated multitrophic aquaculture (IMTA), recirculating aquaculture system (RAS), and beneficial bacteria for aquaculture, have been developed to solve these problems. In this study, the advantages of IMTA and RAS were integrated to develop a novel multitrophic recirculating aquaculture system (MRAS) to adapt to the farm-scale culturing of milkfish (Chanos chanos). The photosynthetic bacteria Rhodovulum sulfidophilum was added to enhance the performance of the farm-scale milkfish MRAS. This setting could promote growth of beneficial bacteria, such as the nitrogen cycle-associated microbial community and the anoxygenic phototrophic Acidobacteria community. The ammonia level was reduced, and the total phosphorous level was stable in the water recycled in the MRAS. The cyanobacteria, algae, Vibrio, Escherichia, and other potential pathogenic bacteria communities were inhibited in the MRAS. This study provides an effective design of a water recycling aquaculture system. Milkfish, Asian tiger shrimp (Penaeus monodon), Asian hard clam (Meretrix lusoria), and seaweed (Gracilaria sp.) can be cultured and simultaneously produced in the system.
Bea-Ven Chang; Chien-Sen Liao; Yi-Tang Chang; Wei-Liang Chao; Shinn-Lih Yeh; Dong-Lin Kuo; Chu-Wen Yang. Investigation of a Farm-scale Multitrophic Recirculating Aquaculture System with the Addition of Rhodovulum sulfidophilum for Milkfish (Chanos chanos) Coastal Aquaculture. Sustainability 2019, 11, 1880 .
AMA StyleBea-Ven Chang, Chien-Sen Liao, Yi-Tang Chang, Wei-Liang Chao, Shinn-Lih Yeh, Dong-Lin Kuo, Chu-Wen Yang. Investigation of a Farm-scale Multitrophic Recirculating Aquaculture System with the Addition of Rhodovulum sulfidophilum for Milkfish (Chanos chanos) Coastal Aquaculture. Sustainability. 2019; 11 (7):1880.
Chicago/Turabian StyleBea-Ven Chang; Chien-Sen Liao; Yi-Tang Chang; Wei-Liang Chao; Shinn-Lih Yeh; Dong-Lin Kuo; Chu-Wen Yang. 2019. "Investigation of a Farm-scale Multitrophic Recirculating Aquaculture System with the Addition of Rhodovulum sulfidophilum for Milkfish (Chanos chanos) Coastal Aquaculture." Sustainability 11, no. 7: 1880.
Tetrabromobisphenol-A (TBBPA) is a pollutant which has a devastating impact on our environment and should be removed from earth. This research aims to evaluate the aerobic and anaerobic TBBPA degradation and bacterial community changes in mangrove sediments. TBBPA degradation in the sediments was enhanced with a microcapsuled enzyme extract of spent mushroom compost (MC) under aerobic conditions and with zerovalent iron under anaerobic conditions. The TBBPA aerobic or anaerobic degradation rates were enhanced for three time additions. Four bacterial genera (Bacillus, Erythrobacter, Pseudomonas, Rhodococcus) were associated with TBBPA aerobic degradation; and four other bacterial genera (Desulfovibrio, Pseudomonas, Sphaerochaeta, Sphingomonas) were associated with TBBPA anaerobic degradation in the sediment. Moreover, nine methanogens were identified under anaerobic conditions that might also be involved in TBBPA anaerobic degradation in the sediment. Our results demonstrate two feasible methods toward TBBPA bioremediation for mangrove sediments under aerobic and anaerobic conditions.
Chu-Wen Yang; Chien-Sen Liao; His Ku; Bea-Ven Chang. Biodegradation of Tetrabromobisphenol-A in Mangrove Sediments. Sustainability 2018, 11, 151 .
AMA StyleChu-Wen Yang, Chien-Sen Liao, His Ku, Bea-Ven Chang. Biodegradation of Tetrabromobisphenol-A in Mangrove Sediments. Sustainability. 2018; 11 (1):151.
Chicago/Turabian StyleChu-Wen Yang; Chien-Sen Liao; His Ku; Bea-Ven Chang. 2018. "Biodegradation of Tetrabromobisphenol-A in Mangrove Sediments." Sustainability 11, no. 1: 151.
Acetaminophen and sulfonamides are emerging contaminants. Conventional wastewater treatment systems fail to degrade these compounds properly. Mycoremediation, is a form of novel bioremediation that uses extracellular enzymes of white-rot fungi to degrade pollutants in the environment. In this study, spent mushroom compost (SMC), which contains fungal extracellular enzymes, was tested for acetaminophen and sulfonamides removal. Among the SMCs of nine mushrooms tested in batch experiments, the SMC of Pleurotus eryngii exhibited the highest removal rate for acetaminophen and sulfonamides. Several fungal extracellular enzymes that might be involved in removal of acetaminophen and sulfonamides were identified by metaproteomic analysis. The bacterial classes, Betaproteobacteria and Alphaproteobacteria, were revealed by metagenomic analysis and may be assisting with acetaminophen and sulfonamide removal, respectively, in the SMC of Pleurotus eryngii. Bioreactor experiments were used to simulate the capability of Pleurotus eryngii SMC for the removal of acetaminophen and sulfonamides from wastewater. The results of this study provide a feasible solution for acetaminophen and sulfonamide removal from wastewater using the SMC of Pleurotus eryngii.
Bea-Ven Chang; Shao-Ning Fan; Yao-Chou Tsai; Yi-Lin Chung; Ping-Xun Tu; Chu-Wen Yang. Removal of emerging contaminants using spent mushroom compost. Science of The Total Environment 2018, 634, 922 -933.
AMA StyleBea-Ven Chang, Shao-Ning Fan, Yao-Chou Tsai, Yi-Lin Chung, Ping-Xun Tu, Chu-Wen Yang. Removal of emerging contaminants using spent mushroom compost. Science of The Total Environment. 2018; 634 ():922-933.
Chicago/Turabian StyleBea-Ven Chang; Shao-Ning Fan; Yao-Chou Tsai; Yi-Lin Chung; Ping-Xun Tu; Chu-Wen Yang. 2018. "Removal of emerging contaminants using spent mushroom compost." Science of The Total Environment 634, no. : 922-933.
Vibrio parahaemolyticus is a human enteropathogenic bacterium and is also pathogenic to shrimp and finfish. In a search for a biocontrol agent for V. parahaemolyticus and other pathogenic Vibrio species, a lytic phage VP06 was isolated from oyster using V. parahaemolyticus as the host. VP06 is a Siphoviridae phage with a polyhedral head and a long tail. The genome sequence of VP06 was 75,893 nucleotides in length and the G+C content was 49%; a total of 101 CDSs were identified in VP06, of which 39 exhibited functional domains/motifs. The genomic sequence of VP06 is similar to those of a lytic V. vulnificus phage SSP002 and a temperate V. parahaemolyticus phage vB_VpaS_MAR10, although VP06 has distinct features in the CDS arrangement and 14 unique CDSs. Phylogenetic analysis revealed that VP06, SSP002 and vB_VpaS_MAR10 belong to a novel genus cluster of Siphoviridae phages. This phage lysed 28.1% of various Vibrio strains, and the efficiency of plating method revealed that VP06 was highly effective in lysing strains of V. alginolyticus, V. azureus, V. harveyi and V. parahaemolyticus. The properties of VP06, including its broad range of hosts and resistance to environmental stresses, indicate that it may be a candidate biocontrol agent.
Hin-Chung Wong; Tzu-Yun Wang; Chu-Wen Yang; Chung-Tao Tang; Chingwen Ying; Chun-Hsiung Wang; Wei-Hau Chang. Characterization of a lytic vibriophage VP06 of Vibrio parahaemolyticus. Research in Microbiology 2018, 170, 13 -23.
AMA StyleHin-Chung Wong, Tzu-Yun Wang, Chu-Wen Yang, Chung-Tao Tang, Chingwen Ying, Chun-Hsiung Wang, Wei-Hau Chang. Characterization of a lytic vibriophage VP06 of Vibrio parahaemolyticus. Research in Microbiology. 2018; 170 (1):13-23.
Chicago/Turabian StyleHin-Chung Wong; Tzu-Yun Wang; Chu-Wen Yang; Chung-Tao Tang; Chingwen Ying; Chun-Hsiung Wang; Wei-Hau Chang. 2018. "Characterization of a lytic vibriophage VP06 of Vibrio parahaemolyticus." Research in Microbiology 170, no. 1: 13-23.
The effects of sucrose and electron acceptors on the anaerobic degradation of sulfamethoxazole (SMX) in mangrove sediments were investigated in this study. Among three sulfonamides, sulfamethoxazole, sulfadimethoxine and sulfamethazine, only SMX could be completely degraded in mangrove sediments. Degradation of SMX was enhanced by the addition of sucrose to the sediments. The degradation rates of SMX were increased in bioreactor experiments with sucrose. The addition of electron acceptors (sodium hydrogen carbonate, sodium sulfate, and sodium nitrate) could further enhance SMX degradation. The order of anaerobic SMX degradation rates under three different conditions was as follows: sulfate-reducing conditions > methanogenic conditions > nitrate-reducing conditions. Methanolobus, Desulfuromonas, and Thauera were found in the highest proportions among methanogens, sulfate-reducing bacteria and denitrifying bacteria, respectively. Achromobacter, Brevundimonas, Delftia, Idiomarina, Pseudomonas, and Rhodopirellula were the major bacterial communities responsible for SMX degradation in the sediment. Overall, 16 bacterial and archaeal genera were identified as the core microbial community facilitating anaerobic SMX degradation for all methanogenic, sulfate-reducing and nitrate-reducing conditions. The results of this study provide feasible methods for the removal of SMX from mangrove sediments.
Chu-Wen Yang; Li-Ling Tsai; Bea-Ven Chang. Anaerobic degradation of sulfamethoxazole in mangrove sediments. Science of The Total Environment 2018, 643, 1446 -1455.
AMA StyleChu-Wen Yang, Li-Ling Tsai, Bea-Ven Chang. Anaerobic degradation of sulfamethoxazole in mangrove sediments. Science of The Total Environment. 2018; 643 ():1446-1455.
Chicago/Turabian StyleChu-Wen Yang; Li-Ling Tsai; Bea-Ven Chang. 2018. "Anaerobic degradation of sulfamethoxazole in mangrove sediments." Science of The Total Environment 643, no. : 1446-1455.
The objective of this study was to evaluate the anaerobic degradation of sulfamethoxazole (SMX) and the associated bacterial community changes in swine and sewage sludges. The degradation rate of SMX was higher in swine sludge than in sewage sludge. The addition of lactate, citrate, and sucrose had significant effects on SMX degradation, and sucrose addition yielded a higher SMX degradation rate than the other additives. At concentrations of 0.1–10 g/l sucrose, the SMX degradation rates increased in the sludge. The bacterial genera from swine sludge with sucrose exhibited the highest SMX degrading efficiency. Seventeen bacterial genera were found to be the major bacterial community members involved in SMX degradation in the sludge.
Chu-Hsi Fan; Chu-Wen Yang; Bea-Ven Chang. Anaerobic degradation of sulfamethoxazole by mixed cultures from swine and sewage sludge. Environmental Technology 2017, 40, 210 -218.
AMA StyleChu-Hsi Fan, Chu-Wen Yang, Bea-Ven Chang. Anaerobic degradation of sulfamethoxazole by mixed cultures from swine and sewage sludge. Environmental Technology. 2017; 40 (2):210-218.
Chicago/Turabian StyleChu-Hsi Fan; Chu-Wen Yang; Bea-Ven Chang. 2017. "Anaerobic degradation of sulfamethoxazole by mixed cultures from swine and sewage sludge." Environmental Technology 40, no. 2: 210-218.
Objective. Curcumin (diferuloylmethane) is a yellow-colored polyphenol with antiproliferative and proapoptotic activities to various types of cancer cells. This study explored the mechanism by which curcumin induces p53-null hepatoma cell apoptosis. Results. AKT, FOXO1, and FOXO3 proteins were downregulated after curcumin treatment. Conversely, PTEN was upregulated. Subcellular fractionations revealed that the FOXO4 protein translocated from cytosol into the nucleus after curcumin treatment. Overexpression of FOXO4 increases the sensitivity of Hep3B cells to curcumin. Knockdown of the FOXO4 gene by siRNA inhibits the proapoptotic effects of curcumin on Hep3B cell. Conclusions. This study revealed the AKT/PTEN/FOXO4 pathway as a potential candidate of target for treatment of p53-null liver cancers.
An-Ting Liou; Mei-Fang Chen; Chu-Wen Yang. Curcumin Induces p53-Null Hepatoma Cell Line Hep3B Apoptosis through the AKT-PTEN-FOXO4 Pathway. Evidence-Based Complementary and Alternative Medicine 2017, 2017, 1 -12.
AMA StyleAn-Ting Liou, Mei-Fang Chen, Chu-Wen Yang. Curcumin Induces p53-Null Hepatoma Cell Line Hep3B Apoptosis through the AKT-PTEN-FOXO4 Pathway. Evidence-Based Complementary and Alternative Medicine. 2017; 2017 ():1-12.
Chicago/Turabian StyleAn-Ting Liou; Mei-Fang Chen; Chu-Wen Yang. 2017. "Curcumin Induces p53-Null Hepatoma Cell Line Hep3B Apoptosis through the AKT-PTEN-FOXO4 Pathway." Evidence-Based Complementary and Alternative Medicine 2017, no. : 1-12.
Our data suggested that there exist different presentations between non-Asian and Taiwanese TNBC subtypes, which provides important information when selection of therapeutic targets or designs for clinical trials for TNBC patients.
Ling-Ming Tseng; Jen-Hwey Chiu; Chun-Yu Liu; Yi-Fang Tsai; Yun-Lin Wang; Chu-Wen Yang; Yi-Ming Shyr. A comparison of the molecular subtypes of triple-negative breast cancer among non-Asian and Taiwanese women. Breast Cancer Research and Treatment 2017, 163, 241 -254.
AMA StyleLing-Ming Tseng, Jen-Hwey Chiu, Chun-Yu Liu, Yi-Fang Tsai, Yun-Lin Wang, Chu-Wen Yang, Yi-Ming Shyr. A comparison of the molecular subtypes of triple-negative breast cancer among non-Asian and Taiwanese women. Breast Cancer Research and Treatment. 2017; 163 (2):241-254.
Chicago/Turabian StyleLing-Ming Tseng; Jen-Hwey Chiu; Chun-Yu Liu; Yi-Fang Tsai; Yun-Lin Wang; Chu-Wen Yang; Yi-Ming Shyr. 2017. "A comparison of the molecular subtypes of triple-negative breast cancer among non-Asian and Taiwanese women." Breast Cancer Research and Treatment 163, no. 2: 241-254.
The LPSBD0 and LPSBD2 peptides, two β-hairpin cationic amphiphilic peptides, exhibit membrane damage and anti-proliferative activities on the A549 (lung cancer) and Hep3B (hepatoma) cell lines were characterized in this study. Light microscopy observations indicate that both peptides induce the production of debris in the cell cultures. The amount of this debris increased in a LPSBD treatment dosage-dependent manner. This debris was also observed by scanning electron microscopy and flow cytometry. As determined by confocal laser microscopy and flow cytometry, cell membrane damage led to Annexin V permeability on the two cancer cell lines used in this study. Both peptide treatments also induced apoptosis in lung and liver cancer cell lines. However, little hemolysis was observed in the hemolytic assay using rat erythrocytes after very-high-dose treatments for both peptides. These results suggest that the two peptides may have the potential to be developed as anti-cancer peptides for human hepatoma and lung cancers.
Hung-Hung Sung; Wann-Neng Jane; Wen-Yi Kao; Po-Ying Fu; Shih-Ching Ho; Chu-Wen Yang. Lipopolysaccharide-Binding Motif Derived Peptides Induce Cell Membrane Damages in Human Lung Cancer and Hepatoma Cell Lines. International Journal of Peptide Research and Therapeutics 2015, 21, 313 -324.
AMA StyleHung-Hung Sung, Wann-Neng Jane, Wen-Yi Kao, Po-Ying Fu, Shih-Ching Ho, Chu-Wen Yang. Lipopolysaccharide-Binding Motif Derived Peptides Induce Cell Membrane Damages in Human Lung Cancer and Hepatoma Cell Lines. International Journal of Peptide Research and Therapeutics. 2015; 21 (3):313-324.
Chicago/Turabian StyleHung-Hung Sung; Wann-Neng Jane; Wen-Yi Kao; Po-Ying Fu; Shih-Ching Ho; Chu-Wen Yang. 2015. "Lipopolysaccharide-Binding Motif Derived Peptides Induce Cell Membrane Damages in Human Lung Cancer and Hepatoma Cell Lines." International Journal of Peptide Research and Therapeutics 21, no. 3: 313-324.
The intensive use of antibiotics may accelerate the development of antibiotic-resistant bacteria (ARB). The global geographical distribution of environmental ARB has been indicated by many studies. However, the ARB in the water environments of Taiwan has not been extensively investigated. The objective of this study was to investigate the communities of ARB in Huanghsi Stream, which presents a natural acidic (pH 4) water environment. Waishuanghsi Stream provides a neutral (pH 7) water environment and was thus also monitored to allow comparison. The plate counts of culturable bacteria in eight antibiotics indicate that the numbers of culturable carbenicillin- and vancomycin-resistant bacteria in both Huanghsi and Waishuanghsi Streams are greater than the numbers of culturable bacteria resistant to the other antibiotics tested. Using a 16S rDNA sequencing approach, both the antibiotic-resistant bacterial communities (culture-based) and the total bacterial communities (metagenome-based) in Waishuanghsi Stream exhibit a higher diversity than those in Huanghsi Stream were observed. Of the three classes of integron, only class I integrons were identified in Waishuanghsi Stream. Our results suggest that an acidic (pH 4) water environment may not only affect the community composition of antibiotic-resistant bacteria but also the horizontal gene transfer mediated by integrons.
Chu-Wen Yang; Yi-Tang Chang; Wei-Liang Chao; Iau-Iun Shiung; Han-Sheng Lin; Hsuan Chen; Szu-Han Ho; Min-Jheng Lu; Pin-Hsuan Lee; Shao-Ning Fan. An investigation of total bacterial communities, culturable antibiotic-resistant bacterial communities and integrons in the river water environments of Taipei city. Journal of Hazardous Materials 2014, 277, 159 -168.
AMA StyleChu-Wen Yang, Yi-Tang Chang, Wei-Liang Chao, Iau-Iun Shiung, Han-Sheng Lin, Hsuan Chen, Szu-Han Ho, Min-Jheng Lu, Pin-Hsuan Lee, Shao-Ning Fan. An investigation of total bacterial communities, culturable antibiotic-resistant bacterial communities and integrons in the river water environments of Taipei city. Journal of Hazardous Materials. 2014; 277 ():159-168.
Chicago/Turabian StyleChu-Wen Yang; Yi-Tang Chang; Wei-Liang Chao; Iau-Iun Shiung; Han-Sheng Lin; Hsuan Chen; Szu-Han Ho; Min-Jheng Lu; Pin-Hsuan Lee; Shao-Ning Fan. 2014. "An investigation of total bacterial communities, culturable antibiotic-resistant bacterial communities and integrons in the river water environments of Taipei city." Journal of Hazardous Materials 277, no. : 159-168.