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Dr. Elisa Crisci
North Carolina State University, Raleigh, North Carolina, USA

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0 Infectious Diseases
0 Virology
0 pig
0 Translational animal models
0 Immunology & immunopathogenesis

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Infectious Diseases

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Short Biography

I think about myself as a "viro-immunologist". I have a veterinary background and I' m working mainly in veterinary immunology and virology, mainly in pigs. I also worked in human viral infection research. I was always interested in innate immunity, mainly cellular responses, against viral infections. I have worked also in complement system, virus-like particles, different RNA and DNA viruses. I promote the use of pig as gold standard animal model for human research.

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Original research article
Published: 24 June 2021 in Frontiers in Veterinary Science
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Diversity, ecology, and evolution of viruses are commonly determined through phylogenetics, an accurate tool for the identification and study of lineages with different pathological characteristics within the same species. In the case of PRRSV, evolutionary research has divided into two main branches based on the use of a specific gene (i.e., ORF5) or whole genome sequences as the input used to produce the phylogeny. In this study, we performed a review on PRRSV phylogenetic literature and characterized the spatiotemporal trends in research of single gene vs. whole genome evolutionary approaches. Finally, using publicly available data, we produced a Bayesian phylodynamic analysis following each research branch and compared the results to determine the pros and cons of each particular approach. This study provides an exploration of the two main phylogenetic research lines applied for PRRSV evolution, as well as an example of the differences found when both methods are applied to the same database. We expect that our results will serve as a guidance for future PRRSV phylogenetic research.

ACS Style

Alba Frias-De-Diego; Manuel Jara; Brittany M. Pecoraro; Elisa Crisci. Whole Genome or Single Genes? A Phylodynamic and Bibliometric Analysis of PRRSV. Frontiers in Veterinary Science 2021, 8, 1 .

AMA Style

Alba Frias-De-Diego, Manuel Jara, Brittany M. Pecoraro, Elisa Crisci. Whole Genome or Single Genes? A Phylodynamic and Bibliometric Analysis of PRRSV. Frontiers in Veterinary Science. 2021; 8 ():1.

Chicago/Turabian Style

Alba Frias-De-Diego; Manuel Jara; Brittany M. Pecoraro; Elisa Crisci. 2021. "Whole Genome or Single Genes? A Phylodynamic and Bibliometric Analysis of PRRSV." Frontiers in Veterinary Science 8, no. : 1.

Immunology
Published: 20 May 2021 in Frontiers in Immunology
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Genital mucosal transmission is the most common route of HIV spread. The initial responses triggered at the site of viral entry are reportedly affected by host factors, especially complement components present at the site, and this will have profound consequences on the outcome and pathogenesis of HIV infection. We studied the initial events associated with host-pathogen interactions by exposing cervical biopsies to free or complement-opsonized HIV. Opsonization resulted in higher rates of HIV acquisition/infection in mucosal tissues and emigrating dendritic cells. Transcriptomic and proteomic data showed a significantly more pathways and higher expression of genes and proteins associated with viral replication and pathways involved in different aspects of viral infection including interferon signaling, cytokine profile and dendritic cell maturation for the opsonized HIV. Moreover, the proteomics data indicate a general suppression by the HIV exposure. This clearly suggests that HIV opsonization alters the initial signaling pathways in the cervical mucosa in a manner that promotes viral establishment and infection. Our findings provide a foundation for further studies of the role these early HIV induced events play in HIV pathogenesis.

ACS Style

Cecilia Svanberg; Rada Ellegård; Elisa Crisci; Mohammad Khalid; Ninnie Borendal Wodlin; Maria Svenvik; Sofia Nyström; Kenzie Birse; Adam Burgener; Esaki M. Shankar; Marie Larsson. Complement-Opsonized HIV Modulates Pathways Involved in Infection of Cervical Mucosal Tissues: A Transcriptomic and Proteomic Study. Frontiers in Immunology 2021, 12, 1 .

AMA Style

Cecilia Svanberg, Rada Ellegård, Elisa Crisci, Mohammad Khalid, Ninnie Borendal Wodlin, Maria Svenvik, Sofia Nyström, Kenzie Birse, Adam Burgener, Esaki M. Shankar, Marie Larsson. Complement-Opsonized HIV Modulates Pathways Involved in Infection of Cervical Mucosal Tissues: A Transcriptomic and Proteomic Study. Frontiers in Immunology. 2021; 12 ():1.

Chicago/Turabian Style

Cecilia Svanberg; Rada Ellegård; Elisa Crisci; Mohammad Khalid; Ninnie Borendal Wodlin; Maria Svenvik; Sofia Nyström; Kenzie Birse; Adam Burgener; Esaki M. Shankar; Marie Larsson. 2021. "Complement-Opsonized HIV Modulates Pathways Involved in Infection of Cervical Mucosal Tissues: A Transcriptomic and Proteomic Study." Frontiers in Immunology 12, no. : 1.

Journal article
Published: 31 January 2021 in Vaccines
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Maternal-derived immunity is a critical component for the survival and success of offspring in pigs to protect from circulating pathogens such as Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV-2). The purpose of this study is to investigate the transfer of anti-PRRSV immunity to piglets from gilts that received modified-live virus (MLV) alone (treatment (TRT) 0), or in combination with one of two autogenous inactivated vaccines (AIVs, TRT 1+2). Piglets from these gilts were challenged with the autogenous PRRSV-2 strain at two weeks of age and their adaptive immune response (IR) was evaluated until 4 weeks post inoculation (wpi). The systemic humoral and cellular IR was analyzed in the pre-farrow gilts, and in piglets, pre-inoculation, and at 2 and 4 wpi. Both AIVs partially protected the piglets with reduced lung pathology and increased weight gain; TRT 1 also lowered piglet viremia, best explained by the AIV-induced production of neutralizing antibodies in gilts and their transfer to the piglets. In piglets, pre-inoculation, the main systemic IFN-γ producers were CD21α+ B cells. From 0 to 4 wpi, the role of these B cells declined and CD4 T cells became the primary systemic IFN-γ producers. In the lungs, CD8 T cells were the primary and CD4 T cells were the secondary IFN-γ producers, including a novel subset of porcine CD8α-CCR7- CD4 T cells, potentially terminally differentiated CD4 TEMRA cells. In summary, this study demonstrates that maternal AIV vaccination can improve protection of pre-weaning piglets against PRRSV-2; it shows the importance of transferring neutralizing antibodies to piglets, and it introduces two novel immune cell subsets in pigs—IFN-γ producing CD21α+ B cells and CD8α-CCR7- CD4 T cells.

ACS Style

Andrew Kick; Zoe Wolfe; Amanda Amaral; Lizette Cortes; Glen Almond; Elisa Crisci; Phillip Gauger; Jeremy Pittman; Tobias Käser. Maternal Autogenous Inactivated Virus Vaccination Boosts Immunity to PRRSV in Piglets. Vaccines 2021, 9, 106 .

AMA Style

Andrew Kick, Zoe Wolfe, Amanda Amaral, Lizette Cortes, Glen Almond, Elisa Crisci, Phillip Gauger, Jeremy Pittman, Tobias Käser. Maternal Autogenous Inactivated Virus Vaccination Boosts Immunity to PRRSV in Piglets. Vaccines. 2021; 9 (2):106.

Chicago/Turabian Style

Andrew Kick; Zoe Wolfe; Amanda Amaral; Lizette Cortes; Glen Almond; Elisa Crisci; Phillip Gauger; Jeremy Pittman; Tobias Käser. 2021. "Maternal Autogenous Inactivated Virus Vaccination Boosts Immunity to PRRSV in Piglets." Vaccines 9, no. 2: 106.

Original research article
Published: 07 December 2020 in Frontiers in Immunology
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Porcine reproductive and respiratory syndrome (PRRS) has an extensive impact on pig production. The causative virus (PRRSV) is divided into two species, PRRSV-1 (European origin) and PRRSV-2 (North American origin). Within PRRSV-1, PRRSV-1.3 strains, such as Lena, are more pathogenic than PRRSV-1.1 strains, such as Flanders 13 (FL13). To date, the molecular interactions of PRRSV with primary lung mononuclear phagocyte (MNP) subtypes, including conventional dendritic cells types 1 (cDC1) and 2 (cDC2), monocyte-derived DCs (moDC), and pulmonary intravascular macrophages (PIM), have not been thoroughly investigated. Here, we analyze the transcriptome profiles of in vivo FL13-infected parenchymal MNP subpopulations and of in vitro FL13- and Lena-infected parenchymal MNP. The cell-specific expression profiles of in vivo sorted cells correlated with their murine counterparts (AM, cDC1, cDC2, moDC) with the exception of PIM. Both in vivo and in vitro, FL13 infection altered the expression of a low number of host genes, and in vitro infection with Lena confirmed the higher ability of this strain to modulate host response. Machine learning (ML) and gene set enrichment analysis (GSEA) unraveled additional relevant genes and pathways modulated by FL13 infection that were not identified by conventional analyses. GSEA increased the cellular pathways enriched in the FL13 data set, but ML allowed a more complete comprehension of functional profiles during FL13 in vitro infection. Data indicates that cellular reprogramming differs upon Lena and FL13 infection and that the latter might keep antiviral and inflammatory macrophage/DC functions silent. Although the slow replication kinetics of FL13 likely contribute to differences in cellular gene expression, the data suggest distinct mechanisms of interaction of the two viruses with the innate immune system during early infection.

ACS Style

Elisa Crisci; Marco Moroldo; Thien-Phong Vu Manh; Ammara Mohammad; Laurent Jourdren; Celine Urien; Edwige Bouguyon; Elise Bordet; Claudia Bevilacqua; Mickael Bourge; Jérémy Pezant; Alexis Pléau; Olivier Boulesteix; Isabelle Schwartz; Nicolas Bertho; Elisabetta Giuffra. Distinctive Cellular and Metabolic Reprogramming in Porcine Lung Mononuclear Phagocytes Infected With Type 1 PRRSV Strains. Frontiers in Immunology 2020, 11, 588411 .

AMA Style

Elisa Crisci, Marco Moroldo, Thien-Phong Vu Manh, Ammara Mohammad, Laurent Jourdren, Celine Urien, Edwige Bouguyon, Elise Bordet, Claudia Bevilacqua, Mickael Bourge, Jérémy Pezant, Alexis Pléau, Olivier Boulesteix, Isabelle Schwartz, Nicolas Bertho, Elisabetta Giuffra. Distinctive Cellular and Metabolic Reprogramming in Porcine Lung Mononuclear Phagocytes Infected With Type 1 PRRSV Strains. Frontiers in Immunology. 2020; 11 ():588411.

Chicago/Turabian Style

Elisa Crisci; Marco Moroldo; Thien-Phong Vu Manh; Ammara Mohammad; Laurent Jourdren; Celine Urien; Edwige Bouguyon; Elise Bordet; Claudia Bevilacqua; Mickael Bourge; Jérémy Pezant; Alexis Pléau; Olivier Boulesteix; Isabelle Schwartz; Nicolas Bertho; Elisabetta Giuffra. 2020. "Distinctive Cellular and Metabolic Reprogramming in Porcine Lung Mononuclear Phagocytes Infected With Type 1 PRRSV Strains." Frontiers in Immunology 11, no. : 588411.

Preprint
Published: 03 December 2020
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Maternal-derived immunity is a critical component for survival and success of offspring in pigs to protect from circulating pathogens like Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV-2). The purpose of this study was to investigate the transfer of anti-PRRSV immunity to piglets from gilts that received modified-live virus (MLV) alone (TRT 0), or in combination with one of two autogenous inactivated vaccines (AIVs, TRT 1+2). Piglets from these gilts were challenged with the autogenous PRRSV-2 strain at two weeks of age and their adaptive immune response (IR) was evaluated until 4 weeks post inoculation (wpi). The systemic humoral and cellular IR was analyzed in the pre-farrow gilts, and in piglets, pre-inoculation, and at 2- and 4-wpi. Both AIVs partially protected the piglets with reduced lung pathology and increased weight gain; TRT 1 also lowered piglet viremia best explained by the AIV-induced production of neutralizing antibodies in gilts and their transfer to the piglets. In piglets, pre-inoculation, the main systemic IFN-γ producers were CD21α+ B cells. From 0 to 4 wpi, the role of these B cells declined and CD4 T cells became the primary systemic IFN-γ producers. In lungs, CD8 T cells were the primary and CD4 T cells the secondary IFN-γ producers including a novel subset of porcine CD8α-CCR7- CD4 T cells, potentially terminally differentiated CD4 TEMRA cells. In summary, this study demonstrates that maternal AIV vaccination can improve protection of pre-weaning piglets against PRRSV-2; it shows the importance of transferring neutralizing antibodies to piglets; and it introduces two novel immune cell subsets in pigs – IFN-γ producing CD21α+ B cells and CD8α-CCR7- CD4 T cells.

ACS Style

Andrew R. Kick; Zoe C. Wolfe; Amanda F. Amaral; Lizette M. Cortes; Glen W. Almond; Elisa Crisci; Phillip C. Gauger; Jeremy Pittman; Tobias Käser. Maternal Autogenous Inactivated Virus Vaccination Boosts the Piglet Humoral and Cell-Mediated Immunity to PRRSV via Transfer of Neutralizing Antibodies and Interferon-Gamma Producing B Cells. 2020, 1 .

AMA Style

Andrew R. Kick, Zoe C. Wolfe, Amanda F. Amaral, Lizette M. Cortes, Glen W. Almond, Elisa Crisci, Phillip C. Gauger, Jeremy Pittman, Tobias Käser. Maternal Autogenous Inactivated Virus Vaccination Boosts the Piglet Humoral and Cell-Mediated Immunity to PRRSV via Transfer of Neutralizing Antibodies and Interferon-Gamma Producing B Cells. . 2020; ():1.

Chicago/Turabian Style

Andrew R. Kick; Zoe C. Wolfe; Amanda F. Amaral; Lizette M. Cortes; Glen W. Almond; Elisa Crisci; Phillip C. Gauger; Jeremy Pittman; Tobias Käser. 2020. "Maternal Autogenous Inactivated Virus Vaccination Boosts the Piglet Humoral and Cell-Mediated Immunity to PRRSV via Transfer of Neutralizing Antibodies and Interferon-Gamma Producing B Cells." , no. : 1.

Journal article
Published: 26 November 2020 in Veterinary Sciences
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Influenza viruses (IV) are one of the major threats to human and animal health worldwide due to the variety of species they affect. Pigs play an important role in IV ecology as the “mixing vessel,” since they can be infected by swine, avian and human IV, allowing the appearance of new subtypes. Human viruses originated in swine are known as IV of swine origin or swine influenza virus (SwIV) variants. In this study, we identified knowledge tendencies of SwIV and assessed potential bias in the literature caused by these variants. We identified the most mentioned SwIV variants and manually reviewed the literature to determine the number of publications applying the whole influenza nomenclature, a partial nomenclature, only the subtype or mixed terminology, along with the proportion of articles in which the GenBank ID number was available. We observed that the 2009 H1N1 human pandemic created an important bias in SwIV research driven by an increase in human publications on the IV of swine origin. H1N1 is the most studied subtype for swine and humans, followed by H3N2. We found differences between the nomenclatures applied, where partial classifications were slightly more common. Finally, from all the publications, only 25% stated the GenBank ID of the sequence studied. This review represents the most complete exploration of trends in SwIV knowledge to date and will serve as a guidance for future search strategies in SwIV research.

ACS Style

Alba Frias-De-Diego; Rachael Posey; Brittany M. Pecoraro; Rafaella Fernandes Carnevale; Alayna Beaty; Elisa Crisci. A Century of Swine Influenza: Is It Really Just about the Pigs? Veterinary Sciences 2020, 7, 189 .

AMA Style

Alba Frias-De-Diego, Rachael Posey, Brittany M. Pecoraro, Rafaella Fernandes Carnevale, Alayna Beaty, Elisa Crisci. A Century of Swine Influenza: Is It Really Just about the Pigs? Veterinary Sciences. 2020; 7 (4):189.

Chicago/Turabian Style

Alba Frias-De-Diego; Rachael Posey; Brittany M. Pecoraro; Rafaella Fernandes Carnevale; Alayna Beaty; Elisa Crisci. 2020. "A Century of Swine Influenza: Is It Really Just about the Pigs?" Veterinary Sciences 7, no. 4: 189.

Journal article
Published: 20 May 2020 in Scientific Reports
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We simultaneously measured the fecal microbiota and multiple environmental and host-related variables in a cohort of 185 healthy horses reared in similar conditions during a period of eight months. The pattern of rare bacteria varied from host to host and was largely different between two time points. Among a suite of variables examined, equitation factors were highly associated with the gut microbiota variability, evoking a relationship between gut microbiota and high levels of physical and mental stressors. Behavioral indicators that pointed toward a compromised welfare state (e.g. stereotypies, hypervigilance and aggressiveness) were also associated with the gut microbiota, reinforcing the notion for the existence of the microbiota-gut-brain axis. These observations were consistent with the microbiability of behaviour traits (> 15%), illustrating the importance of gut microbial composition to animal behaviour. As more elite athletes suffer from stress, targeting the microbiota offers a new opportunity to investigate the bidirectional interactions within the brain gut microbiota axis.

ACS Style

Núria Mach; Alice Ruet; Allison Clark; David Bars-Cortina; Yuliaxis Ramayo-Caldas; Elisa Crisci; Samuel Pennarun; Sophie Dhorne-Pollet; Aline Foury; Marie-Pierre Moisan; Léa Lansade. Priming for welfare: gut microbiota is associated with equitation conditions and behavior in horse athletes. Scientific Reports 2020, 10, 8311 .

AMA Style

Núria Mach, Alice Ruet, Allison Clark, David Bars-Cortina, Yuliaxis Ramayo-Caldas, Elisa Crisci, Samuel Pennarun, Sophie Dhorne-Pollet, Aline Foury, Marie-Pierre Moisan, Léa Lansade. Priming for welfare: gut microbiota is associated with equitation conditions and behavior in horse athletes. Scientific Reports. 2020; 10 (1):8311.

Chicago/Turabian Style

Núria Mach; Alice Ruet; Allison Clark; David Bars-Cortina; Yuliaxis Ramayo-Caldas; Elisa Crisci; Samuel Pennarun; Sophie Dhorne-Pollet; Aline Foury; Marie-Pierre Moisan; Léa Lansade. 2020. "Priming for welfare: gut microbiota is associated with equitation conditions and behavior in horse athletes." Scientific Reports 10, no. 1: 8311.

Journal article
Published: 30 December 2019 in BMC Biology
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Background Comparative genomics studies are central in identifying the coding and non-coding elements associated with complex traits, and the functional annotation of genomes is a critical step to decipher the genotype-to-phenotype relationships in livestock animals. As part of the Functional Annotation of Animal Genomes (FAANG) action, the FR-AgENCODE project aimed to create reference functional maps of domesticated animals by profiling the landscape of transcription (RNA-seq), chromatin accessibility (ATAC-seq) and conformation (Hi-C) in species representing ruminants (cattle, goat), monogastrics (pig) and birds (chicken), using three target samples related to metabolism (liver) and immunity (CD4+ and CD8+ T cells). Results RNA-seq assays considerably extended the available catalog of annotated transcripts and identified differentially expressed genes with unknown function, including new syntenic lncRNAs. ATAC-seq highlighted an enrichment for transcription factor binding sites in differentially accessible regions of the chromatin. Comparative analyses revealed a core set of conserved regulatory regions across species. Topologically associating domains (TADs) and epigenetic A/B compartments annotated from Hi-C data were consistent with RNA-seq and ATAC-seq data. Multi-species comparisons showed that conserved TAD boundaries had stronger insulation properties than species-specific ones and that the genomic distribution of orthologous genes in A/B compartments was significantly conserved across species. Conclusions We report the first multi-species and multi-assay genome annotation results obtained by a FAANG project. Beyond the generation of reference annotations and the confirmation of previous findings on model animals, the integrative analysis of data from multiple assays and species sheds a new light on the multi-scale selective pressure shaping genome organization from birds to mammals. Overall, these results emphasize the value of FAANG for research on domesticated animals and reinforces the importance of future meta-analyses of the reference datasets being generated by this community on different species.

ACS Style

Sylvain Foissac; Sarah Djebali; Kylie Munyard; Nathalie Vialaneix; Andrea Rau; Kevin Muret; Diane Esquerré; Matthias Zytnicki; Thomas Derrien; Philippe Bardou; Fany Blanc; Cédric Cabau; Elisa Crisci; Sophie Dhorne-Pollet; Françoise Drouet; Thomas Faraut; Ignacio Gonzalez; Adeline Goubil; Sonia Lacroix-Lamandé; Fabrice Laurent; Sylvain Marthey; Maria Marti-Marimon; Raphaelle Momal-Leisenring; Florence Mompart; Pascale Quéré; David Robelin; Magali San Cristobal; Gwenola Tosser-Klopp; Silvia Vincent-Naulleau; Stéphane Fabre; Marie-Hélène Pinard-Van Der Laan; Christophe Klopp; Michèle Tixier-Boichard; Hervé Acloque; Sandrine Lagarrigue; Elisabetta Giuffra. Multi-species annotation of transcriptome and chromatin structure in domesticated animals. BMC Biology 2019, 17, 1 -25.

AMA Style

Sylvain Foissac, Sarah Djebali, Kylie Munyard, Nathalie Vialaneix, Andrea Rau, Kevin Muret, Diane Esquerré, Matthias Zytnicki, Thomas Derrien, Philippe Bardou, Fany Blanc, Cédric Cabau, Elisa Crisci, Sophie Dhorne-Pollet, Françoise Drouet, Thomas Faraut, Ignacio Gonzalez, Adeline Goubil, Sonia Lacroix-Lamandé, Fabrice Laurent, Sylvain Marthey, Maria Marti-Marimon, Raphaelle Momal-Leisenring, Florence Mompart, Pascale Quéré, David Robelin, Magali San Cristobal, Gwenola Tosser-Klopp, Silvia Vincent-Naulleau, Stéphane Fabre, Marie-Hélène Pinard-Van Der Laan, Christophe Klopp, Michèle Tixier-Boichard, Hervé Acloque, Sandrine Lagarrigue, Elisabetta Giuffra. Multi-species annotation of transcriptome and chromatin structure in domesticated animals. BMC Biology. 2019; 17 (1):1-25.

Chicago/Turabian Style

Sylvain Foissac; Sarah Djebali; Kylie Munyard; Nathalie Vialaneix; Andrea Rau; Kevin Muret; Diane Esquerré; Matthias Zytnicki; Thomas Derrien; Philippe Bardou; Fany Blanc; Cédric Cabau; Elisa Crisci; Sophie Dhorne-Pollet; Françoise Drouet; Thomas Faraut; Ignacio Gonzalez; Adeline Goubil; Sonia Lacroix-Lamandé; Fabrice Laurent; Sylvain Marthey; Maria Marti-Marimon; Raphaelle Momal-Leisenring; Florence Mompart; Pascale Quéré; David Robelin; Magali San Cristobal; Gwenola Tosser-Klopp; Silvia Vincent-Naulleau; Stéphane Fabre; Marie-Hélène Pinard-Van Der Laan; Christophe Klopp; Michèle Tixier-Boichard; Hervé Acloque; Sandrine Lagarrigue; Elisabetta Giuffra. 2019. "Multi-species annotation of transcriptome and chromatin structure in domesticated animals." BMC Biology 17, no. 1: 1-25.

Original research article
Published: 06 December 2019 in Frontiers in Immunology
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Genital herpes is a common sexually transmitted infection caused by herpes simplex virus type 2 (HSV-2). Genital herpes significantly enhances the acquisition and transmission of HIV-1 by creating a microenvironment that supports HIV infection in the host. Dendritic cells (DCs) represent one of the first innate cell types that encounter HIV-1 and HSV-2 in the genital mucosa. HSV-2 infection has been shown to modulate DCs, rendering them more receptive to HIV infection. Here, we investigated the potential mechanisms underlying HSV-2-mediated augmentation of HIV-1 infection. We demonstrated that the presence of HSV-2 enhanced productive HIV-1 infection of DCs and boosted inflammatory and antiviral responses. The HSV-2 augmented HIV-1 infection required intact HSV-2 DNA, but not active HSV-2 DNA replication. Furthermore, the augmented HIV infection of DCs involved the cGAS-STING pathway. Interestingly, we could not see any involvement of TLR2 or TLR3 nor suppression of infection by IFN-β production. The conditioning by HSV-2 in dual exposed DCs decreased protein expression of IFI16, cGAS, STING, and TBK1, which is associated with signaling through the STING pathway. Dual exposure to HSV-2 and HIV-1 gave decreased levels of several HIV-1 restriction factors, especially SAMHD1, TREX1, and APOBEC3G. Activation of the STING pathway in DCs by exposure to both HSV-2 and HIV-1 most likely led to the proteolytic degradation of the HIV-1 restriction factors SAMHD1, TREX1, and APOBEC3G, which should release their normal restriction of HIV infection in DCs. This released their normal restriction of HIV infection in DCs. We showed that HSV-2 reprogramming of cellular signaling pathways and protein expression levels in the DCs provided a setting where HIV-1 can establish a higher productive infection in the DCs. In conclusion, HSV-2 reprogramming opens up DCs for HIV-1 infection and creates a microenvironment favoring HIV-1 transmission.

ACS Style

Elisa Crisci; Cecilia Svanberg; Rada Ellegård; Mohammad Khalid; Julia Hellblom; Kazuki Okuyama; Pradyot Bhattacharya; Sofia Nyström; Esaki M. Shankar; Kristina Eriksson; Marie Larsson. HSV-2 Cellular Programming Enables Productive HIV Infection in Dendritic Cells. Frontiers in Immunology 2019, 10, 1 .

AMA Style

Elisa Crisci, Cecilia Svanberg, Rada Ellegård, Mohammad Khalid, Julia Hellblom, Kazuki Okuyama, Pradyot Bhattacharya, Sofia Nyström, Esaki M. Shankar, Kristina Eriksson, Marie Larsson. HSV-2 Cellular Programming Enables Productive HIV Infection in Dendritic Cells. Frontiers in Immunology. 2019; 10 ():1.

Chicago/Turabian Style

Elisa Crisci; Cecilia Svanberg; Rada Ellegård; Mohammad Khalid; Julia Hellblom; Kazuki Okuyama; Pradyot Bhattacharya; Sofia Nyström; Esaki M. Shankar; Kristina Eriksson; Marie Larsson. 2019. "HSV-2 Cellular Programming Enables Productive HIV Infection in Dendritic Cells." Frontiers in Immunology 10, no. : 1.

Journal article
Published: 29 August 2019 in Viruses
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Porcine reproductive and respiratory syndrome virus (PRRSV) continues to cause severe reproductive and respiratory pathologies resulting in immense monetary and welfare costs for the swine industry. The vaccines against PRRSV are available; but they struggle with providing protection against the plethora of heterologous PRRSV strains. To improve PRRSV vaccine development, the aim of this study was to provide an in-depth analysis of the crucial heterologous T-cell response to type-2 PRRSV. Following PRRSV modified live virus (MLV) vaccination or infection using one high- or one low-pathogenic PRRSV-strain, this nine-week study evaluated the T-cell response to different PRRSV strains. Our results demonstrate an important role for T cells in this homo- and heterologous response. Specifically, the T-helper cells were the main responders during viremia. Their peak response at 28 dpi correlated with a reduction in viremia, and their homing receptor expression indicated the additional importance for the anti-PRRSV response in the lymphatic and lung tissue. The cytotoxic T lymphocyte (CTL) response was the strongest at the site of infection—the lung and bronchoalveolar lavage. The TCR-γδ T cells were the main responders post viremia and PRRSV induced their expression of the lymph node homing the chemokine receptor, CCR7: This indicates a crucial role for TCR-γδ T cells in the anti-PRRSV response in the lymphatic system.

ACS Style

Andrew R. Kick; Amanda F. Amaral; Lizette M. Cortes; Jonathan E. Fogle; Elisa Crisci; Glen W. Almond; Tobias Käser. The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Viruses 2019, 11, 796 .

AMA Style

Andrew R. Kick, Amanda F. Amaral, Lizette M. Cortes, Jonathan E. Fogle, Elisa Crisci, Glen W. Almond, Tobias Käser. The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Viruses. 2019; 11 (9):796.

Chicago/Turabian Style

Andrew R. Kick; Amanda F. Amaral; Lizette M. Cortes; Jonathan E. Fogle; Elisa Crisci; Glen W. Almond; Tobias Käser. 2019. "The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)." Viruses 11, no. 9: 796.

Original research article
Published: 03 May 2019 in Frontiers in Immunology
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Swine lymph nodes (LN) present an inverted structure compared to mouse and human, with the afferent lymph diffusing from the center to the periphery. This structure, also observed in close and distant species such as dolphins, hippopotamus, rhinoceros, and elephants, is poorly described, nor are the LN macrophage populations and their relationship with B cell follicles. B cell maturation occurs mainly in LN B cell follicles with the help of LN macrophage populations endowed with different antigen delivery capacities. We identified three macrophage populations that we localized in the inverted LN spatial organization. This allowed us to ascribe porcine LN MΦ to their murine counterparts: subcapsular sinus MΦ, medullary cord MΦ and medullary sinus MΦ. We identified the different intra and extrafollicular stages of LN B cells maturation and explored the interaction of MΦ, drained antigen and follicular B cells. The porcine reproductive and respiratory syndrome virus (PRRSV) is a major porcine pathogen that infects tissue macrophages (MΦ). PRRSV is persistent in the secondary lymphoid tissues and induces a delay in neutralizing antibodies appearance. We observed PRRSV interaction with two LN MΦ populations, of which one interacts closely with centroblasts. We observed BCL6 up-regulation in centroblast upon PRRSV infection, leading to new hypothesis on PRRSV inhibition of B cell maturation. This seminal study of porcine LN will permit fruitful comparison with murine and human LN for a better understanding of normal and inverted LN development and functioning.

ACS Style

Elise Bordet; Maxence Frétaud; Elisa Crisci; Edwige Bouguyon; Stéphane Rault; Jérémy Pezant; Alexis Pleau; Patricia Renson; Elisabetta Giuffra; Thibaut Larcher; Mickael Bourge; Olivier Bourry; Olivier Boulesteix; Christelle Langevin; Isabelle Schwartz-Cornil; Nicolas Bertho. Macrophage-B Cell Interactions in the Inverted Porcine Lymph Node and Their Response to Porcine Reproductive and Respiratory Syndrome Virus. Frontiers in Immunology 2019, 10, 953 .

AMA Style

Elise Bordet, Maxence Frétaud, Elisa Crisci, Edwige Bouguyon, Stéphane Rault, Jérémy Pezant, Alexis Pleau, Patricia Renson, Elisabetta Giuffra, Thibaut Larcher, Mickael Bourge, Olivier Bourry, Olivier Boulesteix, Christelle Langevin, Isabelle Schwartz-Cornil, Nicolas Bertho. Macrophage-B Cell Interactions in the Inverted Porcine Lymph Node and Their Response to Porcine Reproductive and Respiratory Syndrome Virus. Frontiers in Immunology. 2019; 10 ():953.

Chicago/Turabian Style

Elise Bordet; Maxence Frétaud; Elisa Crisci; Edwige Bouguyon; Stéphane Rault; Jérémy Pezant; Alexis Pleau; Patricia Renson; Elisabetta Giuffra; Thibaut Larcher; Mickael Bourge; Olivier Bourry; Olivier Boulesteix; Christelle Langevin; Isabelle Schwartz-Cornil; Nicolas Bertho. 2019. "Macrophage-B Cell Interactions in the Inverted Porcine Lymph Node and Their Response to Porcine Reproductive and Respiratory Syndrome Virus." Frontiers in Immunology 10, no. : 953.

Review
Published: 11 March 2019 in Veterinary Sciences
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Porcine respiratory disease complex (PRDC) is a polymicrobial syndrome that results from a combination of infectious agents, such as environmental stressors, population size, management strategies, age, and genetics. PRDC results in reduced performance as well as increased mortality rates and production costs in the pig industry worldwide. This review focuses on the interactions of two enveloped RNA viruses—porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza virus (SwIV)—as major etiological agents that contribute to PRDC within the porcine cellular innate immunity during infection. The innate immune system of the porcine lung includes alveolar and parenchymal/interstitial macrophages, neutrophils (PMN), conventional dendritic cells (DC) and plasmacytoid DC, natural killer cells, and γδ T cells, thus the in vitro and in vivo interactions between those cells and PRRSV and SwIV are reviewed. Likewise, the few studies regarding PRRSV-SwIV co-infection are illustrated together with the different modulation mechanisms that are induced by the two viruses. Alterations in responses by natural killer (NK), PMN, or γδ T cells have not received much attention within the scientific community as their counterpart antigen-presenting cells and there are numerous gaps in the knowledge regarding the role of those cells in both infections. This review will help in paving the way for future directions in PRRSV and SwIV research and enhancing the understanding of the innate mechanisms that are involved during infection with these viruses.

ACS Style

Elisa Crisci; Lorenzo Fraile; Maria Montoya. Cellular Innate Immunity against PRRSV and Swine Influenza Viruses. Veterinary Sciences 2019, 6, 26 .

AMA Style

Elisa Crisci, Lorenzo Fraile, Maria Montoya. Cellular Innate Immunity against PRRSV and Swine Influenza Viruses. Veterinary Sciences. 2019; 6 (1):26.

Chicago/Turabian Style

Elisa Crisci; Lorenzo Fraile; Maria Montoya. 2019. "Cellular Innate Immunity against PRRSV and Swine Influenza Viruses." Veterinary Sciences 6, no. 1: 26.

Journal article
Published: 28 February 2019 in Scientific Reports
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Host miRNAs are known to modulate the cell response to virus infections. We characterized the miRNA-targeted transcriptome of porcine alveolar macrophages (PAMs) at early times after infection with a subtype 1.1 strain of PRRSV (Porcine Reproductive and Respiratory Syndrome Virus). We performed the immunoprecipitation of RISC (RNA-induced Silencing Complex) followed by microarray analysis of the RISC-bound miRNA targets (RIP-Chip) to evaluate the relative enrichment or depletion of expressed genes in RISC. The miRNA-mediated regulation occurred early after PRRSV infection and decreased fast (1,241 and 141 RISC-bound genes at 7 h and 10 h post-infection, respectively); it affected several cell functions with evidence of miRNA buffering of upregulated interferon-related genes. Eight miRNAs were highly enriched in RISC of both control and infected cells with no evidence of differential expression. Although miR-335-5p was the miRNA with most predicted targets among enriched RISC-bound genes, no effects on surface markers, cytokine expression and PRRSV replication were detected upon miR-335-5p mimics of primary PAMs. Our results do not point to specific miRNA-driven mechanisms regulating the early response to infection with this PRRSV 1.1 strain and indicate that the miRNome expressed by steady-state PAMs reacts promptly to counterbalance PRRSV infection by a pervasive modulation of host functions.

ACS Style

Sophie Dhorne-Pollet; Elisa Crisci; Nuria Mach; Patricia Renson; Florence Jaffrézic; Guillemette Marot; Tatiana Maroilley; Marco Moroldo; Jérôme Lecardonnel; Fany Blanc; Nicolas Bertho; Olivier Bourry; Elisabetta Giuffra. The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus. Scientific Reports 2019, 9, 3160 .

AMA Style

Sophie Dhorne-Pollet, Elisa Crisci, Nuria Mach, Patricia Renson, Florence Jaffrézic, Guillemette Marot, Tatiana Maroilley, Marco Moroldo, Jérôme Lecardonnel, Fany Blanc, Nicolas Bertho, Olivier Bourry, Elisabetta Giuffra. The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus. Scientific Reports. 2019; 9 (1):3160.

Chicago/Turabian Style

Sophie Dhorne-Pollet; Elisa Crisci; Nuria Mach; Patricia Renson; Florence Jaffrézic; Guillemette Marot; Tatiana Maroilley; Marco Moroldo; Jérôme Lecardonnel; Fany Blanc; Nicolas Bertho; Olivier Bourry; Elisabetta Giuffra. 2019. "The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus." Scientific Reports 9, no. 1: 3160.

Original research article
Published: 02 October 2018 in Frontiers in Immunology
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Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is an arterivirus responsible for highly contagious infection and huge economic losses in pig industry. Two species, PRRSV-1 and PRRSV-2 are distinguished, PRRSV-1 being more prevalent in Europe. PRRSV-1 can further be divided in subtypes. PRRSV-1.3 such as Lena are more pathogenic than PRRSV-1.1 such as Lelystad or Flanders13. PRRSV-1.3 viruses trigger a higher Th1 response than PRRSV-1.1, although the role of the cellular immune response in PRRSV clearance remains ill defined. The pathogenicity as well as the T cell response inductions may be differentially impacted according to the capacity of the virus strain to infect and/or activate DCs. However, the interactions of PRRSV with in vivo-differentiated-DC subtypes such as conventional DC1 (cDC1), cDC2, and monocyte-derived DCs (moDC) have not been thoroughly investigated. Here, DC subpopulations from Lena in vivo infected pigs were analyzed for viral genome detection. This experiment demonstrates that cDC1, cDC2, and moDC are not infected in vivo by Lena. Analysis of DC cytokines production revealed that cDC1 are clearly activated in vivo by Lena. In vitro comparison of 3 Europeans strains revealed no infection of the cDC1 and cDC2 and no or little infection of moDC with Lena, whereas the two PRRSV-1.1 strains infect none of the 3 DC subtypes. In vitro investigation of T helper polarization and cytokines production demonstrate that Lena induces a higher Th1 polarization and IFNγ secretion than FL13 and LV. Altogether, this work suggests an activation of cDC1 by Lena associated with a Th1 immune response polarization.

ACS Style

Elise Bordet; Fany Blanc; Mathieu Tiret; Elisa Crisci; Edwige Bouguyon; Patricia Renson; Pauline Maisonnasse; Mickael Bourge; Jean-Jacques Leplat; Elisabetta Giuffra; Luc Jouneau; Isabelle Schwartz-Cornil; Olivier Bourry; Nicolas Bertho. Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells. Frontiers in Immunology 2018, 9, 2299 .

AMA Style

Elise Bordet, Fany Blanc, Mathieu Tiret, Elisa Crisci, Edwige Bouguyon, Patricia Renson, Pauline Maisonnasse, Mickael Bourge, Jean-Jacques Leplat, Elisabetta Giuffra, Luc Jouneau, Isabelle Schwartz-Cornil, Olivier Bourry, Nicolas Bertho. Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells. Frontiers in Immunology. 2018; 9 ():2299.

Chicago/Turabian Style

Elise Bordet; Fany Blanc; Mathieu Tiret; Elisa Crisci; Edwige Bouguyon; Patricia Renson; Pauline Maisonnasse; Mickael Bourge; Jean-Jacques Leplat; Elisabetta Giuffra; Luc Jouneau; Isabelle Schwartz-Cornil; Olivier Bourry; Nicolas Bertho. 2018. "Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells." Frontiers in Immunology 9, no. : 2299.

Journal article
Published: 05 July 2018 in Scientific Reports
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Lung inflammation is frequently involved in respiratory conditions and it is strongly controlled by mononuclear phagocytes (MNP). We previously studied porcine lung MNP and described a new population of cells presenting all the features of alveolar macrophages (AM) except for their parenchymal location, that we named AM-like cells. Herein we showed that AM-like cells are macrophages phagocytosing blood-borne particles, in agreement with a pulmonary intravascular macrophages (PIM) identity. PIM have been described microscopically long time ago in species from the Laurasiatheria superorder such as bovine, swine, cats or cetaceans. We observed that PIM were more inflammatory than AM upon infection with the porcine reproductive and respiratory syndrome virus (PRRSV), a major swine pathogen. Moreover, whereas PRRSV was thought to mainly target AM, we observed that PIM were a major producer of virus. The PIM infection was more correlated with viremia in vivo than AM infection. Finally like AM, PIM-expressed genes were characteristic of an embryonic monocyte-derived macrophage population, whose turnover is independent of bone marrow-derived hematopoietic precursors. This last observation raised the interesting possibility that AM and PIM originate from the same lung precursor.

ACS Style

Elise Bordet; Pauline Maisonnasse; Patricia Renson; Edwige Bouguyon; Elisa Crisci; Mathieu Tiret; Delphyne Descamps; Cindy Bernelin-Cottet; Céline Urien; François Lefèvre; Luc Jouneau; Olivier Bourry; Jean-Jacques Leplat; Isabelle Schwartz-Cornil; Nicolas Bertho. Porcine Alveolar Macrophage-like cells are pro-inflammatory Pulmonary Intravascular Macrophages that produce large titers of Porcine Reproductive and Respiratory Syndrome Virus. Scientific Reports 2018, 8, 1 -11.

AMA Style

Elise Bordet, Pauline Maisonnasse, Patricia Renson, Edwige Bouguyon, Elisa Crisci, Mathieu Tiret, Delphyne Descamps, Cindy Bernelin-Cottet, Céline Urien, François Lefèvre, Luc Jouneau, Olivier Bourry, Jean-Jacques Leplat, Isabelle Schwartz-Cornil, Nicolas Bertho. Porcine Alveolar Macrophage-like cells are pro-inflammatory Pulmonary Intravascular Macrophages that produce large titers of Porcine Reproductive and Respiratory Syndrome Virus. Scientific Reports. 2018; 8 (1):1-11.

Chicago/Turabian Style

Elise Bordet; Pauline Maisonnasse; Patricia Renson; Edwige Bouguyon; Elisa Crisci; Mathieu Tiret; Delphyne Descamps; Cindy Bernelin-Cottet; Céline Urien; François Lefèvre; Luc Jouneau; Olivier Bourry; Jean-Jacques Leplat; Isabelle Schwartz-Cornil; Nicolas Bertho. 2018. "Porcine Alveolar Macrophage-like cells are pro-inflammatory Pulmonary Intravascular Macrophages that produce large titers of Porcine Reproductive and Respiratory Syndrome Virus." Scientific Reports 8, no. 1: 1-11.

Research article
Published: 09 October 2017 in PLOS ONE
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Co-infections with human herpesvirus (HHV) have been associated with residual chronic inflammation in antiretroviral (ART)-treated human immunodeficiency virus (HIV)-infected individuals. However, the role of HHV in modulating the tryptophan-kynurenine pathway and clinical outcomes in HIV-infected individuals is poorly understood. Thus, we investigated the seroprevalence of four common HHVs among treated HIV-infected participants and their impact on kynurenine/tryptophan (K/T) ratio and long-term CD4 T-cell recovery in HIV/HHV co-infected participants. In this cross-sectional study, HIV-infected participants receiving suppressive ART for a minimum of 12 months were recruited from the University Malaya Medical Centre (UMMC), Malaysia. Stored plasma was analyzed for CMV, VZV, HSV-1 and HSV-2 IgG antibody levels, immune activation markers (interleukin-6, interferon-γ, neopterin and sCD14), kynurenine and tryptophan concentrations. The influence of the number of HHV co-infection and K/T ratio on CD4 T-cell recovery was assessed using multivariate Poisson regression. A total of 232 HIV-infected participants were recruited and all participants were seropositive for at least one HHV; 96.1% with CMV, 86.6% with VZV, 70.7% with HSV-1 and 53.9% with HSV-2. K/T ratio had a significant positive correlation with CMV (rho = 0.205, p = 0.002), VZV (rho = 0.173, p = 0.009) and a tendency with HSV-2 (rho = 0.120, p = 0.070), with CMV antibody titer demonstrating the strongest modulating effect on K/T ratio among the four HHVs assessed in SOM analysis. In multivariate analysis, higher K/T ratio (p = 0.03) and increasing number of HHV co-infections (p<0.001) were independently associated with poorer CD4 T-cell recovery following 12 months of ART initiation. Multiple HHV co-infections are common among ART-treated HIV-infected participants in the developing country setting and associated with persistent immune activation and poorer CD4 T-cell recovery.

ACS Style

Siew Hwei Yap; Noor Kamila Abdullah; Megan McStea; Kozo Takayama; Meng Li Chong; Elisa Crisci; Marie Larsson; Iskandar Azwa; Adeeba Kamarulzaman; Kok Hoong Leong; Yin Ling Woo; Reena Rajasuriar. HIV/Human herpesvirus co-infections: Impact on tryptophan-kynurenine pathway and immune reconstitution. PLOS ONE 2017, 12, e0186000 .

AMA Style

Siew Hwei Yap, Noor Kamila Abdullah, Megan McStea, Kozo Takayama, Meng Li Chong, Elisa Crisci, Marie Larsson, Iskandar Azwa, Adeeba Kamarulzaman, Kok Hoong Leong, Yin Ling Woo, Reena Rajasuriar. HIV/Human herpesvirus co-infections: Impact on tryptophan-kynurenine pathway and immune reconstitution. PLOS ONE. 2017; 12 (10):e0186000.

Chicago/Turabian Style

Siew Hwei Yap; Noor Kamila Abdullah; Megan McStea; Kozo Takayama; Meng Li Chong; Elisa Crisci; Marie Larsson; Iskandar Azwa; Adeeba Kamarulzaman; Kok Hoong Leong; Yin Ling Woo; Reena Rajasuriar. 2017. "HIV/Human herpesvirus co-infections: Impact on tryptophan-kynurenine pathway and immune reconstitution." PLOS ONE 12, no. 10: e0186000.

Journal article
Published: 01 January 2017 in Revue des Maladies Respiratoires
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ACS Style

P. Maisonnasse; E. Bouguyon; M. Bourge; G. Piton; A. Ezquerra; C. Deloizy; C. Urien; J.-J. Leplat; G. Simon; C. Chevalier; S. Vincent-Naulleau; Elisa Crisci; M. Montoya; I. Schwartz-Cornil; N. Bertho. Pig as a biomedical model: Putting the porcine lung dendritic cells/macrophages network into light. Revue des Maladies Respiratoires 2017, 34, A328 .

AMA Style

P. Maisonnasse, E. Bouguyon, M. Bourge, G. Piton, A. Ezquerra, C. Deloizy, C. Urien, J.-J. Leplat, G. Simon, C. Chevalier, S. Vincent-Naulleau, Elisa Crisci, M. Montoya, I. Schwartz-Cornil, N. Bertho. Pig as a biomedical model: Putting the porcine lung dendritic cells/macrophages network into light. Revue des Maladies Respiratoires. 2017; 34 ():A328.

Chicago/Turabian Style

P. Maisonnasse; E. Bouguyon; M. Bourge; G. Piton; A. Ezquerra; C. Deloizy; C. Urien; J.-J. Leplat; G. Simon; C. Chevalier; S. Vincent-Naulleau; Elisa Crisci; M. Montoya; I. Schwartz-Cornil; N. Bertho. 2017. "Pig as a biomedical model: Putting the porcine lung dendritic cells/macrophages network into light." Revue des Maladies Respiratoires 34, no. : A328.

Journal article
Published: 23 August 2016 in Scientific Reports
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Virus-like particles (VLPs), comprised of viral structural proteins devoid of genetic material, are tunable nanoparticles that can be chemically or genetically engineered, to be used as platforms for multimeric display of foreign antigens. Here, we report the engineering of chimeric VLPs, derived from rabbit hemorrhagic disease virus (RHDV) for presentation of foreign B-cell antigens to the immune system. The RHDV capsid comprises 180 copies of a single capsid subunit (VP60). To evaluate the ability of chimeric RHDV VLPs to elicit protective humoral responses against foreign antigens, we tested two B-cell epitopes: a novel neutralizing B-cell epitope, derived from feline calicivirus capsid protein, and a well characterized B-cell epitope from the extracellular domain of influenza A virus M2 protein (M2e). We generated sets of chimeric RHDV VLPs by insertion of the foreign B-cell epitopes at three different locations within VP60 protein (which involved different levels of surface accessibility) and in different copy numbers per site. The immunogenic potential of the chimeric VLPs was analyzed in the mouse model. The results presented here indicated that chimeric RHDV VLPs elicit potent protective humoral responses against displayed foreign B-cell epitopes, demonstrated by both, in vitro neutralization and in vivo protection against a lethal challenge.

ACS Style

Noelia Moreno; Ignacio Mena; Iván Angulo; Yolanda Gómez; Elisa Crisci; Maria Montoya; Jose Caston; Esther Blanco; Juan Bárcena. Rabbit hemorrhagic disease virus capsid, a versatile platform for foreign B-cell epitope display inducing protective humoral immune responses. Scientific Reports 2016, 6, 31844 .

AMA Style

Noelia Moreno, Ignacio Mena, Iván Angulo, Yolanda Gómez, Elisa Crisci, Maria Montoya, Jose Caston, Esther Blanco, Juan Bárcena. Rabbit hemorrhagic disease virus capsid, a versatile platform for foreign B-cell epitope display inducing protective humoral immune responses. Scientific Reports. 2016; 6 (1):31844.

Chicago/Turabian Style

Noelia Moreno; Ignacio Mena; Iván Angulo; Yolanda Gómez; Elisa Crisci; Maria Montoya; Jose Caston; Esther Blanco; Juan Bárcena. 2016. "Rabbit hemorrhagic disease virus capsid, a versatile platform for foreign B-cell epitope display inducing protective humoral immune responses." Scientific Reports 6, no. 1: 31844.

Article
Published: 15 May 2016 in Journal of Virology
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Herpes simplex virus 2 (HSV-2) is one of the most common sexually transmitted infections globally, with a very high prevalence in many countries. During HSV-2 infection, viral particles become coated with complement proteins and antibodies, both present in genital fluids, which could influence the activation of immune responses. In genital mucosa, the primary target cells for HSV-2 infection are epithelial cells, but resident immune cells, such as dendritic cells (DCs), are also infected. DCs are the activators of the ensuing immune responses directed against HSV-2, and the aim of this study was to examine the effects opsonization of HSV-2, either with complement alone or with complement and antibodies, had on the infection of immature DCs and their ability to mount inflammatory and antiviral responses. Complement opsonization of HSV-2 enhanced both the direct infection of immature DCs and their production of new infectious viral particles. The enhanced infection required activation of the complement cascade and functional complement receptor 3. Furthermore, HSV-2 infection of DCs required endocytosis of viral particles and their delivery into an acid endosomal compartment. The presence of complement in combination with HSV-1- or HSV-2-specific antibodies more or less abolished HSV-2 infection of DCs. Our results clearly demonstrate the importance of studying HSV-2 infection under conditions that ensue in vivo , i.e., conditions under which the virions are covered in complement fragments and complement fragments and antibodies, as these shape the infection and the subsequent immune response and need to be further elucidated. IMPORTANCE During HSV-2 infection, viral particles should become coated with complement proteins and antibodies, both present in genital fluids, which could influence the activation of the immune responses. The dendritic cells are activators of the immune responses directed against HSV-2, and the aim of this study was to examine the effects of complement alone or complement and antibodies on HSV-2 infection of dendritic cells and their ability to mount inflammatory and antiviral responses. Our results demonstrate that the presence of antibodies and complement in the genital environment can influence HSV-2 infection under in vitro conditions that reflect the in vivo situation. We believe that our findings are highly relevant for the understanding of HSV-2 pathogenesis.

ACS Style

Elisa Crisci; Rada Ellegård; Sofia Nyström; Elin Rondahl; Lena Serrander; Tomas Bergström; Christopher Sjöwall; Kristina Eriksson; Marie Larsson. Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells. Journal of Virology 2016, 90, 4939 -4950.

AMA Style

Elisa Crisci, Rada Ellegård, Sofia Nyström, Elin Rondahl, Lena Serrander, Tomas Bergström, Christopher Sjöwall, Kristina Eriksson, Marie Larsson. Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells. Journal of Virology. 2016; 90 (10):4939-4950.

Chicago/Turabian Style

Elisa Crisci; Rada Ellegård; Sofia Nyström; Elin Rondahl; Lena Serrander; Tomas Bergström; Christopher Sjöwall; Kristina Eriksson; Marie Larsson. 2016. "Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells." Journal of Virology 90, no. 10: 4939-4950.

Article report
Published: 04 November 2015 in Mucosal Immunology
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Human and mouse respiratory tracts show anatomical and physiological differences, which will benefit from alternative experimental models for studying many respiratory diseases. Pig has been recognized as a valuable biomedical model, in particular for lung transplantation or pathologies such as cystic fibrosis and influenza infection. However, there is a lack of knowledge about the porcine respiratory immune system. Here we segregated and studied six populations of pig lung dendritic cells (DCs)/macrophages (Mθs) as follows: conventional DCs (cDC) 1 and cDC2, inflammatory monocyte-derived DCs (moDCs), monocyte-derived Mθs, and interstitial and alveolar Mθs. The three DC subsets present migratory and naive T-cell stimulation capacities. As observed in human and mice, porcine cDC1 and cDC2 were able to induce T-helper (Th)1 and Th2 responses, respectively. Interestingly, porcine moDCs increased in the lung upon influenza infection, as observed in the mouse model. Pig cDC2 shared some characteristics observed in human but not in mice, such as the expression of FCɛRIα and Langerin, and an intra-epithelial localization. This work, by unraveling the extended similarities of the porcine and human lung DC/Mθ networks, highlights the relevance of pig, both as an exploratory model of DC/Mθ functions and as a model for human inflammatory lung pathologies.

ACS Style

P Maisonnasse; E Bouguyon; G Piton; Angel Ezquerra; C Urien; C Deloizy; M Bourge; J-J Leplat; Gaëlle Simon; C Chevalier; S Vincent-Naulleau; Elisa Crisci; M Montoya; I Schwartz-Cornil; N Bertho. The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model. Mucosal Immunology 2015, 9, 835 -849.

AMA Style

P Maisonnasse, E Bouguyon, G Piton, Angel Ezquerra, C Urien, C Deloizy, M Bourge, J-J Leplat, Gaëlle Simon, C Chevalier, S Vincent-Naulleau, Elisa Crisci, M Montoya, I Schwartz-Cornil, N Bertho. The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model. Mucosal Immunology. 2015; 9 (4):835-849.

Chicago/Turabian Style

P Maisonnasse; E Bouguyon; G Piton; Angel Ezquerra; C Urien; C Deloizy; M Bourge; J-J Leplat; Gaëlle Simon; C Chevalier; S Vincent-Naulleau; Elisa Crisci; M Montoya; I Schwartz-Cornil; N Bertho. 2015. "The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model." Mucosal Immunology 9, no. 4: 835-849.