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This study aimed to evaluate the effects of taxifolin and sorghum ethanol extract on free fatty acid (FFA)-induced hepatic insulin resistance. FFA treatment decreased glucose uptake by 16.2% compared with that in the control, whereas taxifolin and sorghum ethanol extract increased the glucose uptake. Additionally, taxifolin and sorghum ethanol extract increased the expression of p-PI3K, p-IRS1, p-AKT, p-AMPK, and p-ACC in FFA-induced hepatocytes. Furthermore, FFA treatment increased the expression of miR-195. However, compared with the FFA treatment, treatment with taxifolin and sorghum ethanol extract decreased miR-195 expression in a dose-dependent manner. Taxifolin and sorghum ethanol extract enhanced p-IRS1, p-PI3K, p-AMPK, p-AKT, and p-ACC expression by suppressing miR-195 levels in miR-195 mimic- or inhibitor-transfected cells. These results indicate that taxifolin and sorghum ethanol extract attenuate insulin resistance by regulating miR-195 expression, which suggests that taxifolin and sorghum ethanol extract may be useful antidiabetic agents.
Hana Lee; Won-Tae Jeong; Yoon-Sup So; Heung-Bin Lim; Junsoo Lee. Taxifolin and Sorghum Ethanol Extract Protect against Hepatic Insulin Resistance via the miR-195/IRS1/PI3K/AKT and AMPK Signalling Pathways. Antioxidants 2021, 10, 1331 .
AMA StyleHana Lee, Won-Tae Jeong, Yoon-Sup So, Heung-Bin Lim, Junsoo Lee. Taxifolin and Sorghum Ethanol Extract Protect against Hepatic Insulin Resistance via the miR-195/IRS1/PI3K/AKT and AMPK Signalling Pathways. Antioxidants. 2021; 10 (9):1331.
Chicago/Turabian StyleHana Lee; Won-Tae Jeong; Yoon-Sup So; Heung-Bin Lim; Junsoo Lee. 2021. "Taxifolin and Sorghum Ethanol Extract Protect against Hepatic Insulin Resistance via the miR-195/IRS1/PI3K/AKT and AMPK Signalling Pathways." Antioxidants 10, no. 9: 1331.
Amaranthus species are widely used as grain and leaf vegetables around the world and are potential dietary sources of antioxidants and phenolic compounds. In this study, we examined the variation in total flavonoid contents, total polyphenol contents, and antioxidant activities among 120 accessions of nine Amaranthus species. The antioxidant activity of DPPH (2,2-diphenyl-1-picrylhydrazyl) of the 120 amaranth accessions ranged from 1.1 (A. tricolor) to 75.2 (A. tricolor mg ascorbic acid equivalents (AAE)/g in 2018, and 8.5 (A. tricolor) to 68.8 (A. dubius) mg AAE/g in 2019. ABTS (2,2′-azinobis (3-ethylbenzothiazoline 6-sulfonate)) antioxidant activity ranged from 16.7 (A. tricolor) to 78.3 (A. hypochondriacus) mg AAE/g in 2018, and 36.6 (A. tricolor) to 79.2 (A. dubius) mg AAE/g in 2019. Total flavonoid content (TFC) of 2018 and 2019 ranged from 21.7 (A. caudatus) to 52.7 (A. hybridus) and from 22.3 (A. viridis) to 54.7 (A. tricolor), respectively Antioxidant activities were compared using two methods and all components were measured in plants grown both in 2018 and 2019. We identified wide variation among the accessions and between plants grown in the two years. Antioxidant activities and phytochemical contents were consistently negatively correlated. The nine species and 120 accessions clustered into three groups according to their antioxidant activities, total flavonoid contents, and total polyphenol contents in each year. These results provide information about the nutritional profiles of different Amaranthus species.
Jun-Hyoung Bang; Kyung Lee; Won Jeong; Seahee Han; Ick-Hyun Jo; Seong Choi; Hyunwoo Cho; Tae Hyun; Jeehye Sung; Junsoo Lee; Yoon-Sup So; Jong-Wook Chung. Antioxidant Activity and Phytochemical Content of Nine Amaranthus Species. Agronomy 2021, 11, 1032 .
AMA StyleJun-Hyoung Bang, Kyung Lee, Won Jeong, Seahee Han, Ick-Hyun Jo, Seong Choi, Hyunwoo Cho, Tae Hyun, Jeehye Sung, Junsoo Lee, Yoon-Sup So, Jong-Wook Chung. Antioxidant Activity and Phytochemical Content of Nine Amaranthus Species. Agronomy. 2021; 11 (6):1032.
Chicago/Turabian StyleJun-Hyoung Bang; Kyung Lee; Won Jeong; Seahee Han; Ick-Hyun Jo; Seong Choi; Hyunwoo Cho; Tae Hyun; Jeehye Sung; Junsoo Lee; Yoon-Sup So; Jong-Wook Chung. 2021. "Antioxidant Activity and Phytochemical Content of Nine Amaranthus Species." Agronomy 11, no. 6: 1032.
Black soybean (Glycine max L.) has been used as a traditional medicine because its seed coat contains various natural phenolic compounds such as anthocyanins. The objective of this study was to reveal the genetic variation in the agricultural traits, phytochemicals, and antioxidant activity of 172 Korean black soybean landraces (KBSLs) and establish a relationship among them. The evaluation of three agricultural traits (days to 50% flowering, maturity, and 100-seed weight), six phytochemicals (delphinidin-3-glucoside, cyaniding-3-glucoside, petunidin-3-glucoside, daidzin, glycitin, and genestin), and four antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), ferric-reducing antioxidant power (FRAP), and the total polyphenol content (TPC) of 172 KBSLs were analyzed in 2012 and 2015. The agricultural traits, phytochemicals, and antioxidant activities of the 172 KBSLs showed wide variation among the accessions and years. In correlation analysis, the agricultural traits and phytochemicals showed positive and negative correlations with phytochemicals and antioxidant activity, respectively. The principal component analyses result indicated that phytochemicals accounted for most of the variability in the KBSLs. In clustering analysis, the 172 KBSLs were classified into four clusters. These results could lead to expanding the knowledge of the agricultural traits, phytochemicals, and antioxidant activity of the KBSLs, which are valuable materials for the development of new soybean varieties.
Kyung Jun Lee; Da-Young Baek; Gi-An Lee; Gyu-Taek Cho; Yoon-Sup So; Jung-Ro Lee; Kyung-Ho Ma; Jong-Wook Chung; Do Yoon Hyun. Phytochemicals and Antioxidant Activity of Korean Black Soybean (Glycine max L.) Landraces. Antioxidants 2020, 9, 213 .
AMA StyleKyung Jun Lee, Da-Young Baek, Gi-An Lee, Gyu-Taek Cho, Yoon-Sup So, Jung-Ro Lee, Kyung-Ho Ma, Jong-Wook Chung, Do Yoon Hyun. Phytochemicals and Antioxidant Activity of Korean Black Soybean (Glycine max L.) Landraces. Antioxidants. 2020; 9 (3):213.
Chicago/Turabian StyleKyung Jun Lee; Da-Young Baek; Gi-An Lee; Gyu-Taek Cho; Yoon-Sup So; Jung-Ro Lee; Kyung-Ho Ma; Jong-Wook Chung; Do Yoon Hyun. 2020. "Phytochemicals and Antioxidant Activity of Korean Black Soybean (Glycine max L.) Landraces." Antioxidants 9, no. 3: 213.
Licorice (Glycyrrhiza glabra) is an important medicinal herb and has long been used in traditional medicine for the treatment of several diseases worldwide. Understanding the genetic diversity within Glycyrrhiza species is important for the efficient conservation of these medicinal herbs. In this study, we have developed 20 polymorphic chloroplast microsatellite (cpSSR) markers using the chloroplast genome of G. lepidota. The cpSSR markers were tested on a total of 27 Glycyrrhiza individual plants. The number of alleles per locus ranged from two to eight among the Glycyrrhiza accessions. Overall, the Shannon index (I) for each cpSSR ranged from 0.315 to 1.694, the diversity indices (h) were 0.140–0.793 and the unbiased diversity indices (uh) were 0.145–0.825. In addition, the cpSSR markers were successfully divided and classified the 27 Glycyrrhiza individuals into four groups. The cpSSR markers developed in this study could be used in the assessment of genetic diversity and rapid identification of Glycyrrhiza species.
Kyung Jun Lee; Sebastin Raveendar; Ji Seon Choi; Jinsu Gil; Jeong Hoon Lee; Yoon-Sup So; Jong-Wook Chung. Development of chloroplast microsatellite markers for identification of Glycyrrhiza species. Plant Genetic Resources: Characterization and Utilization 2018, 17, 95 -98.
AMA StyleKyung Jun Lee, Sebastin Raveendar, Ji Seon Choi, Jinsu Gil, Jeong Hoon Lee, Yoon-Sup So, Jong-Wook Chung. Development of chloroplast microsatellite markers for identification of Glycyrrhiza species. Plant Genetic Resources: Characterization and Utilization. 2018; 17 (1):95-98.
Chicago/Turabian StyleKyung Jun Lee; Sebastin Raveendar; Ji Seon Choi; Jinsu Gil; Jeong Hoon Lee; Yoon-Sup So; Jong-Wook Chung. 2018. "Development of chloroplast microsatellite markers for identification of Glycyrrhiza species." Plant Genetic Resources: Characterization and Utilization 17, no. 1: 95-98.
The white button mushroom (Agaricus bisporus) is one of the most widely cultivated species of edible mushroom. Despite its economic importance, relatively little is known about the genetic diversity of this species. Illumina paired-end sequencing produced 43,871,558 clean reads and 69,174 contigs were generated from five offspring. These contigs were subsequently assembled into 57,594 unigenes. The unigenes were annotated with reference genome in which 6,559 unigenes were associated with clusters, indicating orthologous genes. Gene ontology classification assigned many unigenes. Based on genome data of the five offspring, 44 polymorphic simple sequence repeat (SSR) markers were developed. The major allele frequency ranged from 0.42 to 0.92. The number of genotypes and the number of alleles ranged from 1 to 4, and from 2 to 4, respectively. The observed heterozygosity and the expected heterozygosity ranged from 0.00 to 1.00, and from 0.15 to 0.64, respectively. The polymorphic information content value ranged from 0.14 to 0.57. The genetic distances and UPGMA clustering discriminated offspring strains. The SSR markers developed in this study can be applied in polymorphism analyses of button mushroom and for cultivar discrimination.
Hwa-Yong Lee; Sebastin Raveendar; Hyejin An; Youn-Lee Oh; Kab-Yeul Jang; Won-Sik Kong; Hojin Ryu; Yoon-Sup So; Jong-Wook Chung. Development of Polymorphic Simple Sequence Repeat Markers using High-Throughput Sequencing in Button Mushroom (Agaricus bisporus). Mycobiology 2018, 46, 421 -428.
AMA StyleHwa-Yong Lee, Sebastin Raveendar, Hyejin An, Youn-Lee Oh, Kab-Yeul Jang, Won-Sik Kong, Hojin Ryu, Yoon-Sup So, Jong-Wook Chung. Development of Polymorphic Simple Sequence Repeat Markers using High-Throughput Sequencing in Button Mushroom (Agaricus bisporus). Mycobiology. 2018; 46 (4):421-428.
Chicago/Turabian StyleHwa-Yong Lee; Sebastin Raveendar; Hyejin An; Youn-Lee Oh; Kab-Yeul Jang; Won-Sik Kong; Hojin Ryu; Yoon-Sup So; Jong-Wook Chung. 2018. "Development of Polymorphic Simple Sequence Repeat Markers using High-Throughput Sequencing in Button Mushroom (Agaricus bisporus)." Mycobiology 46, no. 4: 421-428.