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Nutrire publishes new evidence for clinical nutrition practice, advancing basic and experimental nutrition and food science research, and providing the ...
Flavia Rodrigues Da Silva; Antonio Herbert Lancha Junior; Valdênio Martins Brant; Ingrid Ludimila Bastos Lôbo; Luciana Oquendo Pereira Lancha; Andressa Silva; Marco Túlio De Mello. The effects of COVID-19 quarantine on eating and sleeping behaviors. Nutrire 2020, 45, 1 .
AMA StyleFlavia Rodrigues Da Silva, Antonio Herbert Lancha Junior, Valdênio Martins Brant, Ingrid Ludimila Bastos Lôbo, Luciana Oquendo Pereira Lancha, Andressa Silva, Marco Túlio De Mello. The effects of COVID-19 quarantine on eating and sleeping behaviors. Nutrire. 2020; 45 (2):1.
Chicago/Turabian StyleFlavia Rodrigues Da Silva; Antonio Herbert Lancha Junior; Valdênio Martins Brant; Ingrid Ludimila Bastos Lôbo; Luciana Oquendo Pereira Lancha; Andressa Silva; Marco Túlio De Mello. 2020. "The effects of COVID-19 quarantine on eating and sleeping behaviors." Nutrire 45, no. 2: 1.
In December 2019, Wuhan, in China, attracted international attention due to a pneumonia outbreak caused by the new coronavirus (2019-nCoV). Infection by 2019-nCoV is more likely in elderly people with comorbidities or with associated chronic diseases. Due to the high transmission rate among humans, this disease is rapidly disseminated, which led to several events being canceled, including the Tokyo 2020 Olympic and Paralympic Games. The aim of this article is to discuss the risk factors for Olympic and Paralympic athletes, as well as for spectators, that justify the decision to postpone the Tokyo Games 2020. Regular physical exercise is associated with health and the prevention of chronic diseases. Although athletes generally appear to be healthy and physically fit, this may not be true. The immune system, which protects the organism from invasive microorganisms, can be affected by the duration and quality of sleep, as well as by physical exercise which influences the quality of the immune response. High volumes of high-intensity physical exercise, as well as changes in sleep patterns during the pre-competition period and the impacts of jet lag on athletes traveling for the Tokyo Games in 2020 may lead to immune system suppression, making these groups more vulnerable to infection by 2019-nCoV. Moreover, during the period planned for the games in 2020 the pandemic may be subsiding in some countries and increasing in others, and this was also taken into consideration as a risk factor. Hence, the decision taken to postpone the Tokyo 2020 Olympic and Paralympic Games until 2021 due to the 2019-nCoV was the correct one, and was extremely important to protect the health of Olympic and Paralympic athletes, as well as spectators. Level of evidence V; expert opinion .
Renato De Carvalho Guerreiro; Andressa Silva; Henrique De Araújo Andrade; Isadora Grade Biasibetti; Roberto Vital; Hesojy Gley Vital Da Silva; Flavia Rodrigues Da Silva; Marco Túlio De Mello. WAS POSTPONING THE TOKYO 2020 OLYMPIC AND PARALYMPIC GAMES A CORRECT DECISION? Revista Brasileira de Medicina do Esporte 2020, 26, 191 -195.
AMA StyleRenato De Carvalho Guerreiro, Andressa Silva, Henrique De Araújo Andrade, Isadora Grade Biasibetti, Roberto Vital, Hesojy Gley Vital Da Silva, Flavia Rodrigues Da Silva, Marco Túlio De Mello. WAS POSTPONING THE TOKYO 2020 OLYMPIC AND PARALYMPIC GAMES A CORRECT DECISION? Revista Brasileira de Medicina do Esporte. 2020; 26 (3):191-195.
Chicago/Turabian StyleRenato De Carvalho Guerreiro; Andressa Silva; Henrique De Araújo Andrade; Isadora Grade Biasibetti; Roberto Vital; Hesojy Gley Vital Da Silva; Flavia Rodrigues Da Silva; Marco Túlio De Mello. 2020. "WAS POSTPONING THE TOKYO 2020 OLYMPIC AND PARALYMPIC GAMES A CORRECT DECISION?" Revista Brasileira de Medicina do Esporte 26, no. 3: 191-195.
Rotating and permanent night shiftwork schedules typically result in acute and sometimes chronic sleep deprivation plus acute and sometimes chronic disruption of the circadian time structure. Immune system processes and functionalities are organized as circadian rhythms, and they are also strongly influenced by sleep status. Sleep is a vital behavioral state of living beings and a modulator of immune function and responsiveness. Shiftworkers show increased risk for developing viral infections due to possible compromise of both innate and acquired immunity responses. Short sleep and sleep loss, common consequences of shiftwork, are associated with altered integrity of the immune system. We discuss the possible excess risk for COVID-19 infection in the context of the common conditions among shiftworkers, including nurses, doctors, and first responders, among others of high exposure to the contagion, of sleep imbalance and circadian disruption. Abbreviations: ACE2: Angiotensin-converting enzyme 2; APC: Antigen-presenting cells; CCL: Chemokine (C-C motif) ligand; CD+: Adhesion molecule expression; COVID-19: 2019 coronavirus disease; DCs: Dendritic cells; GH: Growth hormone; HPA: Hypothalamic-pituitary-adrenal; HSF: Heat shock factor; HSP70: Heat shock protein 70; HSP90: Heat shock protein 90; IL: Interleukin; INFγ: Interferon-gamma; LT/LB: T/B lymphocytes; MHC: Major histocompatibility complex; NK: Natural killer; RAAS: renin–angiotensin–aldosterone system; SARS: Severe acute respiratory syndrome; SCN: Suprachiasmatic nucleus;SD: Sleep deprivation; SNS: Sympathetic nervous system; Th1/Th2: T helper lymphocytes 1/2; TLR2/TLR4: Toll-like receptor 2/4; TNF-α: Tumor necrosis factor alpha; VEGF: Vascular endothelial growth factor
Flavia Rodrigues da Silva; Renato De Carvalho Guerreiro; Henrique De Araújo Andrade; Eduardo Stieler; Andressa Silva; Marco Túlio de Mello. Does the compromised sleep and circadian disruption of night and shiftworkers make them highly vulnerable to 2019 coronavirus disease (COVID-19)? Chronobiology International 2020, 37, 607 -617.
AMA StyleFlavia Rodrigues da Silva, Renato De Carvalho Guerreiro, Henrique De Araújo Andrade, Eduardo Stieler, Andressa Silva, Marco Túlio de Mello. Does the compromised sleep and circadian disruption of night and shiftworkers make them highly vulnerable to 2019 coronavirus disease (COVID-19)? Chronobiology International. 2020; 37 (5):607-617.
Chicago/Turabian StyleFlavia Rodrigues da Silva; Renato De Carvalho Guerreiro; Henrique De Araújo Andrade; Eduardo Stieler; Andressa Silva; Marco Túlio de Mello. 2020. "Does the compromised sleep and circadian disruption of night and shiftworkers make them highly vulnerable to 2019 coronavirus disease (COVID-19)?" Chronobiology International 37, no. 5: 607-617.
Chemical ocular burns are among the most frequently eye-related injuries, which require immediate and intensive evaluation and care since they may lead to potential complications such as superinfection, corneal perforation, and blindness.Vasconcellea cundinamarcensis, a species from Caricaceae family, contains highly active proteolytic enzymes in its latex that show healing activity in animal models bearing lesions of different etiologies. We evaluate the ocular toxicity of the proteolytic fraction from V. cundinamarcensis (P1G10) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Hen's Egg Test-Chorioallantoic Membrane test. The corneal healing property of P1G10 was studied by the ethanol-chemical burn in the rabbit's eyes. P1G10 is safe for ocular administration, except when administrated at 10μg/mL. P1G10 at 1μg/mL accelerates the corneal re-epithelization achieving complete wound closure after 72h of chemical burn. Also, P1G10 modulated the inflammatory response and controlled the arrangement of collagen fibers in the stroma, demonstrating its potential corneal healing properties. Our work was the first one to evaluate the ophthalmic application of P1G10. Here we demonstrated that P1G10 is suitable for ocular administration and it has a promising corneal healing activity which may emerge as a new pharmacological tool to the development of a new drug for ocular surface chemical injuries in the future.
Rummenigge Oliveira Silva; Bruna Lopes Da Costa; Carolina Nunes Da Silva; Thaís Maria Da Mata Martins; Lays Fernanda Nunes Dourado; Alfredo Miranda De Goes; Miriam Teresa Lopes; Carlos Edmundo Salas; Armando Cunha; Flavia Rodrigues Da Silva. The proteolytic fraction from Vasconcellea cundinamarcensis accelerates wound healing after corneal chemical burn in rabbits. Burns 2019, 46, 928 -936.
AMA StyleRummenigge Oliveira Silva, Bruna Lopes Da Costa, Carolina Nunes Da Silva, Thaís Maria Da Mata Martins, Lays Fernanda Nunes Dourado, Alfredo Miranda De Goes, Miriam Teresa Lopes, Carlos Edmundo Salas, Armando Cunha, Flavia Rodrigues Da Silva. The proteolytic fraction from Vasconcellea cundinamarcensis accelerates wound healing after corneal chemical burn in rabbits. Burns. 2019; 46 (4):928-936.
Chicago/Turabian StyleRummenigge Oliveira Silva; Bruna Lopes Da Costa; Carolina Nunes Da Silva; Thaís Maria Da Mata Martins; Lays Fernanda Nunes Dourado; Alfredo Miranda De Goes; Miriam Teresa Lopes; Carlos Edmundo Salas; Armando Cunha; Flavia Rodrigues Da Silva. 2019. "The proteolytic fraction from Vasconcellea cundinamarcensis accelerates wound healing after corneal chemical burn in rabbits." Burns 46, no. 4: 928-936.
Bacterial keratitis is an ocular infection that can lead to severe visual disability. Staphylococcus aureus is a major pathogen of the eye. We recently demonstrated the strong antimicrobial activity of LyeTxI-b, a synthetic peptide derived from a Lycosa erithrognatha toxin. Herein, we evaluated a topical formulation (eye drops) containing LyeTxI-b to treat resistant bacterial keratitis. Keratitis was induced with intrastromal injection of 4 × 105 cells (4 µL) in New Zealand female white rabbits. Minimum inhibitory concentration (MIC) and biofilm viability were determined. LyeTxI-b ocular toxicity was evaluated through chorioallantoic membrane and Draize tests. One drop of the formulation (LyeTxI-b 28.9 µmol/L +0.5% CMC in 0.9% NaCl) was instilled into each eye four times a day, for a week. Slit-lamp biomicroscopy analysis, corneal histopathological studies and cellular infiltrate quantification through myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) detection were performed. LyeTxI-b was very effective in the treatment of keratitis, with no signs of ocular toxicity. Planktonic bacteria MIC was 3.6 µmol/L and LyeTxI-b treatment reduced biofilm viability in 90%. LyeTxI-b eliminated bacteria and reduced inflammatory cellular activity in the eyes. Healthy and treated animals showed similar NAG and MPO levels. LyeTxI-b is a potent new drug to treat resistant bacterial keratitis, showing effective antimicrobial and anti-inflammatory activity.
Carolina Nunes Da Silva; Flavia Rodrigues Da Silva; Lays Fernanda Nunes Dourado; Pablo Victor Mendes Dos Reis; Rummenigge Oliveira Silva; Bruna Lopes Da Costa; Paula Santos Nunes; Flávio Almeida Amaral; Vera Lúcia Dos Santos; Maria Elena De Lima; Armando Da Silva Cunha Júnior. A New Topical Eye Drop Containing LyeTxI-b, A Synthetic Peptide Designed from A Lycosa erithrognata Venom Toxin, Was Effective to Treat Resistant Bacterial Keratitis. Toxins 2019, 11, 203 .
AMA StyleCarolina Nunes Da Silva, Flavia Rodrigues Da Silva, Lays Fernanda Nunes Dourado, Pablo Victor Mendes Dos Reis, Rummenigge Oliveira Silva, Bruna Lopes Da Costa, Paula Santos Nunes, Flávio Almeida Amaral, Vera Lúcia Dos Santos, Maria Elena De Lima, Armando Da Silva Cunha Júnior. A New Topical Eye Drop Containing LyeTxI-b, A Synthetic Peptide Designed from A Lycosa erithrognata Venom Toxin, Was Effective to Treat Resistant Bacterial Keratitis. Toxins. 2019; 11 (4):203.
Chicago/Turabian StyleCarolina Nunes Da Silva; Flavia Rodrigues Da Silva; Lays Fernanda Nunes Dourado; Pablo Victor Mendes Dos Reis; Rummenigge Oliveira Silva; Bruna Lopes Da Costa; Paula Santos Nunes; Flávio Almeida Amaral; Vera Lúcia Dos Santos; Maria Elena De Lima; Armando Da Silva Cunha Júnior. 2019. "A New Topical Eye Drop Containing LyeTxI-b, A Synthetic Peptide Designed from A Lycosa erithrognata Venom Toxin, Was Effective to Treat Resistant Bacterial Keratitis." Toxins 11, no. 4: 203.
The great diversity of molecules found in spider venoms include amino acids, polyamines, proteins and peptides, among others. Some of these compounds can interact with different neuronal receptors and ion channels including those present in the ocular system. To study potential toxicity and safety of intravitreal injection in rabbits of LyeTx I b, a synthetic peptide derived from the toxin LyeTx I found in venom from the spider Lycosa eritrognatha and to evaluate the angiogenic activity on a CAM model. ARPE-19 cells were treated with LyeTx I b (0.36; 0.54; 0.72; 2.89; 4.34 or 9.06 μM). In this study, New Zealand rabbits were used. LyeTx I b (2.89 μM) labeled with FITC dissolved in PBS, or only PBS, were injected into vitreous humor. Electroretinogram (ERG) was recorded 1 day before injection and at 7, 14 and 28 days post-injection. Clinical examination of the retina was conducted through tonometer and eye fundus after ERG. Eyes were enucleated and retinas were prepared for histology in order to assess retinal structure. CAMs were exposed to LyeTx I b (0.54; 0.72; 2.17 or 2.89 μM). ARPE-19 cells exposed to LyeTx I b showed cell viability at the same levels of the control. The fluorescence of LyeTx I b labeled with FITC indicated its retinal localization. Our findings indicate ERG responses from rats injected in the eye with LyeTx I b were very similar to the corresponding responses of those animals injected only with vehicle. Clinical examination found no alterations of intraocular pressure or retinal integrity. No histological damage in retinal layers was observed. CAM presented reduced neovascularization when exposed to LyeTx I b. Intravitreal injection of LyeTx I b is safe for use in the rabbit eye and prevents neovascularization in the CAM model, at Bevacizumab levels. These findings support intravitreal LyeTx I b as a good candidate to develop future alternative treatment for the retina in neovascularization diseases.
Flavia Rodrigues Da Silva; Mayara Rodrigues Brandão De Paiva; Lays Fernanda Nunes Dourado; Rummenigge Oliveira Silva; Carolina Nunes Da Silva; Bruna Lopes Da Costa; Cibele Toledo; Maria Elena De Lima; Armando Da Silva-Cunha. Intravitreal injection of the synthetic peptide LyeTx I b, derived from a spider toxin, into the rabbit eye is safe and prevents neovascularization in a chorio-allantoic membrane model. Journal of Venomous Animals and Toxins including Tropical Diseases 2018, 24, 31 .
AMA StyleFlavia Rodrigues Da Silva, Mayara Rodrigues Brandão De Paiva, Lays Fernanda Nunes Dourado, Rummenigge Oliveira Silva, Carolina Nunes Da Silva, Bruna Lopes Da Costa, Cibele Toledo, Maria Elena De Lima, Armando Da Silva-Cunha. Intravitreal injection of the synthetic peptide LyeTx I b, derived from a spider toxin, into the rabbit eye is safe and prevents neovascularization in a chorio-allantoic membrane model. Journal of Venomous Animals and Toxins including Tropical Diseases. 2018; 24 (1):31.
Chicago/Turabian StyleFlavia Rodrigues Da Silva; Mayara Rodrigues Brandão De Paiva; Lays Fernanda Nunes Dourado; Rummenigge Oliveira Silva; Carolina Nunes Da Silva; Bruna Lopes Da Costa; Cibele Toledo; Maria Elena De Lima; Armando Da Silva-Cunha. 2018. "Intravitreal injection of the synthetic peptide LyeTx I b, derived from a spider toxin, into the rabbit eye is safe and prevents neovascularization in a chorio-allantoic membrane model." Journal of Venomous Animals and Toxins including Tropical Diseases 24, no. 1: 31.
Spider toxins are recognized as useful sources of bioactive substances, showing a wide range of pharmacological effects on neurotransmission. Several spider toxins have been identified biochemically and some of them are specific glutamate receptors antagonists. Previous data indicate that PnTx4-5-5, a toxin isolated from the spider Phoneutria nigriventer, inhibits the N-methyl-d-aspartate receptor (NMDAR), with little or no effect on AMPA, kainate or GABA receptors. In agreement with these results, our findings in this study show that PnTx4-5-5 reduces the amplitude of NMDAR-mediated EPSCs in hippocampal slices. It is well established that glutamate-mediated excitotoxic neuronal cell death occurs mainly via NMDAR activation. Thus, we decided to investigate whether PnTx4-5-5 would protect against various cell death insults. For that, we used primary-cultured corticostriatal neurons from wild type (WT) mice, as well as from a mouse model of Huntington's disease, BACHD. Our results showed that PnTx4-5-5 promotes neuroprotection of WT and BACHD neurons under the insult of high levels of glutamate. Moreover, the toxin is also able to protect WT neurons against amyloid β (Aβ) peptide toxicity. These results indicate that the toxin PnTx4-5-5 is a potential neuroprotective drug.
Flavia R. Silva; Edleusa M.L. Batista; Marcus V. Gomez; Christopher Kushmerick; Juliana F. Da Silva; Marta N. Cordeiro; Luciene B. Vieira; Fabiola M. Ribeiro. The Phoneutria nigriventer spider toxin, PnTx4-5-5, promotes neuronal survival by blocking NMDA receptors. Toxicon 2016, 112, 16 -21.
AMA StyleFlavia R. Silva, Edleusa M.L. Batista, Marcus V. Gomez, Christopher Kushmerick, Juliana F. Da Silva, Marta N. Cordeiro, Luciene B. Vieira, Fabiola M. Ribeiro. The Phoneutria nigriventer spider toxin, PnTx4-5-5, promotes neuronal survival by blocking NMDA receptors. Toxicon. 2016; 112 ():16-21.
Chicago/Turabian StyleFlavia R. Silva; Edleusa M.L. Batista; Marcus V. Gomez; Christopher Kushmerick; Juliana F. Da Silva; Marta N. Cordeiro; Luciene B. Vieira; Fabiola M. Ribeiro. 2016. "The Phoneutria nigriventer spider toxin, PnTx4-5-5, promotes neuronal survival by blocking NMDA receptors." Toxicon 112, no. : 16-21.