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Dr. work ZŁOTKOWSKA
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland

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0 Dendritic Cells
0 Food allergens
0 Mucosal Immunity
0 T cells, B cells, myeloid cells

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Journal article
Published: 18 June 2021 in International Journal of Molecular Sciences
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Chicken meat is often a major component of a modern diet. Allergy to chicken meat is relatively rare and occurs independently or in subjects allergic to ovalbumin (OVA). We examined the effect of adoptive transfer of OVA-CD4+ T cells on the immune response to OVA in mice fed chicken meat. Donor mice were injected intraperitoneally with 100 µg of OVA with Freund’s adjuvant two times over a week, and CD4+ T cells were isolated from them and transferred to naïve mice (CD4+/OVA/ChM group), which were then provoked with OVA with FA and fed freeze-dried chicken meat for 14 days. The mice injected with OVA and fed chicken meat (OVA/ChM group), and sensitized (OVA group) and healthy (PBS group) mice served as controls. Humoral and cellular response to OVA was monitored over the study. The CD4+/OVA/ChM group had lowered levels of anti-OVA IgG and IgA, and total IgE. There were significant differences in CD4+, CD4+CD25+, and CD4+CD25+Foxp3+ T cells between groups. OVA stimulation decreased the splenocyte proliferation index and IFN-γ secretion in the CD4+/OVA/ChM group compared to the OVA group. IL-4 was increased in the OVA/ChM mice, which confirms allergenic potential of the egg–meat protein combination. Transfer of OVA-experienced CD4+ T cells ameliorated the negative immune response to OVA.

ACS Style

Ewa Fuc; Dagmara Złotkowska; Ewa Wasilewska; Barbara Wróblewska. OVA-Experienced CD4+ T Cell Transfer and Chicken Protein Challenge Affect the Immune Response to OVA in a Murine Model. International Journal of Molecular Sciences 2021, 22, 6573 .

AMA Style

Ewa Fuc, Dagmara Złotkowska, Ewa Wasilewska, Barbara Wróblewska. OVA-Experienced CD4+ T Cell Transfer and Chicken Protein Challenge Affect the Immune Response to OVA in a Murine Model. International Journal of Molecular Sciences. 2021; 22 (12):6573.

Chicago/Turabian Style

Ewa Fuc; Dagmara Złotkowska; Ewa Wasilewska; Barbara Wróblewska. 2021. "OVA-Experienced CD4+ T Cell Transfer and Chicken Protein Challenge Affect the Immune Response to OVA in a Murine Model." International Journal of Molecular Sciences 22, no. 12: 6573.

Journal article
Published: 25 January 2021 in Nutrients
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The presence of various proteins, including modified ones, in food which exhibit diverse immunogenic and sensitizing properties increases the difficulty of predicting host immune responses. Still, there is a lack of sufficiently reliable and comparable data and research models describing allergens in dietary matrices. The aim of the study was to estimate the immunomodulatory effects of β-lactoglobulin (β-lg) in comparison to those elicited by κ-casein (κ-CN), in vivo and ex vivo, using naïve splenocytes and a mouse sensitization model. Our results revealed that the humoral and cellular responses triggered by β-lg and κ-CN were of diverse magnitudes and showed different dynamics in the induction of control mechanisms. β-Lg turned out to be more immunogenic and induced a more dominant Th1 response than κ-CN, which triggered a significantly higher IgE response. For both proteins, CD4+ lymphocyte profiles correlated with CD4+CD25+ and CD4+CD25+Foxp3+ T cells induction and interleukin 10 secretion, but β-lg induced more CD4+CD25+Foxp3- Tregs. Moreover, ex vivo studies showed the risk of interaction of immune responses to different milk proteins, which may exacerbate allergy, especially the one caused by β-lg. In conclusion, the applied model of in vivo and ex vivo exposure to β-lg and κ-CN showed significant differences in immunoreactivity of the tested proteins (κ-CN demonstrated stronger allergenic potential than β-lg), and may be useful for the estimation of allergenic potential of various food proteins, including those modified in technological processes.

ACS Style

Dagmara Złotkowska; Emilia Stachurska; Ewa Fuc; Barbara Wróblewska; Anita Mikołajczyk; Ewa Wasilewska. Differences in Regulatory Mechanisms Induced by β-Lactoglobulin and κ-Casein in Cow’s Milk Allergy Mouse Model–In Vivo and Ex Vivo Studies. Nutrients 2021, 13, 349 .

AMA Style

Dagmara Złotkowska, Emilia Stachurska, Ewa Fuc, Barbara Wróblewska, Anita Mikołajczyk, Ewa Wasilewska. Differences in Regulatory Mechanisms Induced by β-Lactoglobulin and κ-Casein in Cow’s Milk Allergy Mouse Model–In Vivo and Ex Vivo Studies. Nutrients. 2021; 13 (2):349.

Chicago/Turabian Style

Dagmara Złotkowska; Emilia Stachurska; Ewa Fuc; Barbara Wróblewska; Anita Mikołajczyk; Ewa Wasilewska. 2021. "Differences in Regulatory Mechanisms Induced by β-Lactoglobulin and κ-Casein in Cow’s Milk Allergy Mouse Model–In Vivo and Ex Vivo Studies." Nutrients 13, no. 2: 349.

Journal article
Published: 04 November 2020 in Nutrients
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There is no effective therapy for milk allergy. The role of lactic acid bacteria (LAB) and probiotics in protection against allergy-related outcomes is still under investigation. The aim of the study was to evaluate the immunomodulative and therapeutic potential of yogurt drinks in cow’s milk allergy (CMA) management. We compared immunoreactivity of α-casein (α-CN), β-casein (β-CN), κ-casein (κ-CN), α-lactalbumin (α-LA), and β-lactoglobulin (β-LG) in 27 yogurt drinks fermented with different basic yogurt cultures, or yogurt cultures enriched with Lactobacillus plantarum and/or Bifidobacterium lactis strains, by competitive ELISA assay. Drinks with the lowest antigenic potential were used as allergoids for CMA therapy. BALB/c mice were sensitized via intraperitoneal injection of α-CN + β-LG mixture with aluminum adjuvant, and gavaged with increasing doses of selected low-immunogenic drinks (YM—basic, or YM-LB—enriched with L. plantarum and B. lactis) to induce tolerance. Milk- or phosphate-buffered saline (PBS)-dosed mice served as controls. Compared to milk, the immunoreactivity of proteins in drinks increased or decreased, depending on the bacterial sets applied for fermentation. Only a few sets acted synergistically in reducing immunoreactivity. The selected low-immunogenic drinks stimulated allergic mice for profiling Th2 to Th1 response and acquire tolerance, and the effect was greater with YM-LB drink, which during long-lasting interventional feeding strongly increased the secretion of regulatory cytokines, i.e., IL-10 and TGF-β, and IgA and decreased IL-4, IgE, and anti-(α-CN + β-LG) IgG1. The studies revealed variations in the potency of yogurt bacteria to change allergenicity of milk proteins and the need for their strict selection to obtain a safe product for allergy sufferers. The YM-LB drink with reduced antigenic potential may be a source of allergoids used in the immunotherapy of IgE mediated CMA, but further clinical or volunteer studies are required.

ACS Style

Barbara Wróblewska; Anna Kaliszewska-Suchodoła; Ewa Fuc; Lidia Markiewicz; Anna Ogrodowczyk; Dagmara Złotkowska; Ewa Wasilewska. Effect of Low-Immunogenic Yogurt Drinks and Probiotic Bacteria on Immunoreactivity of Cow’s Milk Proteins and Tolerance Induction—In Vitro and In Vivo Studies. Nutrients 2020, 12, 3390 .

AMA Style

Barbara Wróblewska, Anna Kaliszewska-Suchodoła, Ewa Fuc, Lidia Markiewicz, Anna Ogrodowczyk, Dagmara Złotkowska, Ewa Wasilewska. Effect of Low-Immunogenic Yogurt Drinks and Probiotic Bacteria on Immunoreactivity of Cow’s Milk Proteins and Tolerance Induction—In Vitro and In Vivo Studies. Nutrients. 2020; 12 (11):3390.

Chicago/Turabian Style

Barbara Wróblewska; Anna Kaliszewska-Suchodoła; Ewa Fuc; Lidia Markiewicz; Anna Ogrodowczyk; Dagmara Złotkowska; Ewa Wasilewska. 2020. "Effect of Low-Immunogenic Yogurt Drinks and Probiotic Bacteria on Immunoreactivity of Cow’s Milk Proteins and Tolerance Induction—In Vitro and In Vivo Studies." Nutrients 12, no. 11: 3390.

Research article
Published: 16 March 2020 in Journal of Agricultural and Food Chemistry
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Green pea (Pisum sativum) is a component of European cuisine; however, an estimated 0.8% of Europeans suffer from allergies to pea proteins. We examined the immunoreactive potential of pea albumins in BALB/c and C57BL/6 mice. Mice were orally gavaged with pea albumins (PA) or glycated pea albumins (G-PA) for 10 consecutive days, in combination with an adjuvant. Both PA and G-PA increased PA-specific serum antibodies titres to about 212 for anti-PA IgG, ~27 for anti-PA IgA, and ~27.8for anti-PA IgA in faecal extracts (p < 0.001). On day 42 post-exposure, the antibodies titres decreased and were greater in BALB/c compared to C57BL/6 mice (p < 0.05). Distribution of CD4+ and CD8+ T cells in lymphoid tissues presented strain-specific differences. PA was found to induce lymphocyte proliferation, however, G-PA did not. Both PA and G-PA changed CD4+ and CD8+ T cell percentages in some lymphoid tissues; however, this did not impact cytokines production by splenocyte cultures evidenced by the stimulation of Th1, Th2, and Th17 cells. The observed immunomodulatory properties of PA and G-PA and no sign of allergic reaction render them suitable for supplements in personalized diets, but further research is needed to precisely understand this activity.

ACS Style

Justyna Patrycja Chudzik-Kozłowska; Ewa Wasilewska; Dagmara Zlotkowska. Evaluation of Immunoreactivity of Pea (Pisum sativum) Albumins in BALB/c and C57BL/6 Mice. Journal of Agricultural and Food Chemistry 2020, 68, 3891 -3902.

AMA Style

Justyna Patrycja Chudzik-Kozłowska, Ewa Wasilewska, Dagmara Zlotkowska. Evaluation of Immunoreactivity of Pea (Pisum sativum) Albumins in BALB/c and C57BL/6 Mice. Journal of Agricultural and Food Chemistry. 2020; 68 (13):3891-3902.

Chicago/Turabian Style

Justyna Patrycja Chudzik-Kozłowska; Ewa Wasilewska; Dagmara Zlotkowska. 2020. "Evaluation of Immunoreactivity of Pea (Pisum sativum) Albumins in BALB/c and C57BL/6 Mice." Journal of Agricultural and Food Chemistry 68, no. 13: 3891-3902.

Journal article
Published: 04 September 2019 in Nutrients
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The mechanism of food allergy may vary. This study aimed to compare the effects of milk, yogurt, or beef meat supplementation on humoral and cellular immune responses in a mice model. Mice were divided into four groups: The "Milk group" was sensitized with a β-lactoglobulin (β-lg)/α-casein (α-CN) mixture and supplemented cow milk; the "Yogurt group" was sensitized with β-lg/α-CN and supplemented yogurt; the "Beef group" was immunized with bovine serum albumin (BSA) and supplemented beef meat; and the "PBS group" received PBS in all procedures. ELISA was used to measure humoral response, including: Total IgE, specific IgG, and IgA. Cellular response was determined by phenotyping lymphocyte from lymphoid tissue and measuring the Th1/Th2 cytokine concentration with flow cytometry. The qPCR method was used for quantification of the fecal microbiota. The results obtained revealed a lower IgE level for the Yogurt group than for the Milk one. In the Yogurt group, the contribution of regulatory T cells to MLN and PP was higher compared to the other groups. We confirmed that diet supplementation with yogurt modulates the immune response to the prime allergen, and changes the activity of serum antibodies to milk proteins and BSA. Based on a specific antibodies level, we cannot exclude the possibility of CMA mice reaction against BSA.

ACS Style

Ewa Fuc; Dagmara Złotkowska; Barbara Wróblewska. Milk and Meat Allergens from Bos taurus β-Lactoglobulin, α-Casein, and Bovine Serum Albumin: An In-Vivo Study of the Immune Response in Mice. Nutrients 2019, 11, 2095 .

AMA Style

Ewa Fuc, Dagmara Złotkowska, Barbara Wróblewska. Milk and Meat Allergens from Bos taurus β-Lactoglobulin, α-Casein, and Bovine Serum Albumin: An In-Vivo Study of the Immune Response in Mice. Nutrients. 2019; 11 (9):2095.

Chicago/Turabian Style

Ewa Fuc; Dagmara Złotkowska; Barbara Wróblewska. 2019. "Milk and Meat Allergens from Bos taurus β-Lactoglobulin, α-Casein, and Bovine Serum Albumin: An In-Vivo Study of the Immune Response in Mice." Nutrients 11, no. 9: 2095.

Journal article
Published: 25 April 2019 in BMC Neuroscience
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Despite increasing evidence that lipopolysaccharide (LPS) affects the biological active substances of dorsal root ganglia (DRG) we have limited knowledge of the influence of a single low dose of LPS, which does not result in any clinical symptoms of disease (subclinical LPS) on neuropeptides connected with the sensory pathway. Accordingly, in this work, we investigated the influence of subclinical LPS from Salmonella Enteritidis on selected neuropeptides: substance P (SP), galanin (GAL), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP) and somatostatin (SOM) in the cervical, thoracic, lumbar and sacral regions of the DRG and spinal cord. This study was performed on immature female pigs of the Pietrain × Duroc breed. Seven days after the intravenous injection of saline solution for control animals (n = 5) and 5 μg/kg b.w. LPS from S. Enteritidis for the experimental group (n = 5), the DRG and the spinal cord were collected to extract the neuropeptides using solid-phase extraction technology. Our results demonstrated that subclinical LPS in DRG was able to change the levels of all studied neuropeptides except SOM, whereas in the spinal cord it down-regulated all studied neuropeptides in the sacral spinal cord, maintaining the concentration of all studied neuropeptides in other regions similar to that observed in the control animals. The significant differences in the intensity and character of observed changes between particular regions of the DRG suggest that the exact functions of the studied neuropeptides and mechanisms of responses to subclinical LPS action depend on specific characteristics and functions of each examination region of DRG. The mechanisms of observed changes are not fully understood and require further study of the molecular interactions between subclinical LPS from S. Enteritidis and neuronal and non-neuronal cells of DRG and spinal cord. The peripheral and central pain pathways must be analysed with the aspect of unknown long-term consequences of the influence of subclinical LPS from S. Enteritidis on neuropeptides in the spinal cord and the dorsal root ganglia.

ACS Style

Anita Mikołajczyk; Dagmara Złotkowska. Subclinical lipopolysaccharide from Salmonella Enteritidis induces neuropeptide dysregulation in the spinal cord and the dorsal root ganglia. BMC Neuroscience 2019, 20, 18 .

AMA Style

Anita Mikołajczyk, Dagmara Złotkowska. Subclinical lipopolysaccharide from Salmonella Enteritidis induces neuropeptide dysregulation in the spinal cord and the dorsal root ganglia. BMC Neuroscience. 2019; 20 (1):18.

Chicago/Turabian Style

Anita Mikołajczyk; Dagmara Złotkowska. 2019. "Subclinical lipopolysaccharide from Salmonella Enteritidis induces neuropeptide dysregulation in the spinal cord and the dorsal root ganglia." BMC Neuroscience 20, no. 1: 18.

Journal article
Published: 03 March 2019 in Bone
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Bone metabolism disturbances are commonly observed in patients with newly diagnosed celiac disease (CD). The only available treatment for CD–the intake of a gluten-free diet (GFD)–has been found to be insufficient in effectively improving bone health in some patients. Therefore, there is an urgent need to modify the GFD so as to allow for the provision of all the necessary nutrients and improved absorption. Prebiotics intake reportedly improves the absorption of bone-related vitamin D and calcium as well as bone metabolism. The effect of prebiotic intake on bone health in CD patients has not been studied yet. This study aimed to evaluate the effect of oligofructose-enriched inulin intake on bone metabolism and immune response in children with CD on a GFD. A total of 34 children with CD were randomised into two groups receiving 10 g of oligofructose-enriched inulin (Synergy 1) or a placebo (maltodextrin) for three months, together with a strict GFD. The children's bone metabolism marker levels and cytokine profiles were analysed before and after the intervention. After supplementation, the concentration of osteocalcin increased significantly in children receiving Synergy 1, while the concentration of bone alkaline phosphatase increased in both groups, independent of supplementation. After the intervention, the level of pyridinoline increased significantly in the placebo group, resulting in a concentration that was two times higher than that in the Synergy 1 group, in which it remained stable. Moreover, the plasma concentrations of N-terminal telopeptides of type I collagen decreased in both the groups, whereas the tartrate-resistant acid phosphatase 5b level increased particularly in the Synergy 1 group. The intervention did not lead to immunological response changes. The proposed supplementation beneficially altered bone metabolism, through increased bone formation rates and decreased bone resorption process rates. Supplementation of GFD with prebiotic oligofructose-enriched inulin may be a promising auxiliary therapy for bone metabolism improvements in children with CD.

ACS Style

Natalia Drabińska; Elżbieta Jarocka-Cyrta; Dagmara Złotkowska; Paweł Abramowicz; Urszula Krupa-Kozak. Daily oligofructose-enriched inulin intake impacts bone turnover markers but not the cytokine profile in pediatric patients with celiac disease on a gluten-free diet: Results of a randomised, placebo-controlled pilot study. Bone 2019, 122, 184 -192.

AMA Style

Natalia Drabińska, Elżbieta Jarocka-Cyrta, Dagmara Złotkowska, Paweł Abramowicz, Urszula Krupa-Kozak. Daily oligofructose-enriched inulin intake impacts bone turnover markers but not the cytokine profile in pediatric patients with celiac disease on a gluten-free diet: Results of a randomised, placebo-controlled pilot study. Bone. 2019; 122 ():184-192.

Chicago/Turabian Style

Natalia Drabińska; Elżbieta Jarocka-Cyrta; Dagmara Złotkowska; Paweł Abramowicz; Urszula Krupa-Kozak. 2019. "Daily oligofructose-enriched inulin intake impacts bone turnover markers but not the cytokine profile in pediatric patients with celiac disease on a gluten-free diet: Results of a randomised, placebo-controlled pilot study." Bone 122, no. : 184-192.

Journal article
Published: 02 February 2019 in Toxins
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Bacterial lipopolysaccharide (LPS) can contribute to the pathogenesis and the clinical symptoms of many diseases such as cancer, mental disorders, neurodegenerative as well as metabolic diseases. The asymptomatic carrier state of Salmonella spp. is a very important public health problem. A subclinical single dose of LPS obtained from S. Enteritidis (5 μg/kg, i.v.) was administered to discern the consequences of changes of various brain peptides such as corticotropin-releasing hormone (CRH), gonadotropin-releasing hormone (GnRH), thyrotropin-releasing hormone (TRH), galanin (GAL), neuropeptide Y (NPY), somatostatin (SOM), substance P (SP), and vasoactive intestinal polypeptide (VIP) in selected clinically important brain sections and endocrine glands of the hypothalamic-pituitary-adrenal (HPA), -thyroid (HPT), -ovarian (HPO) axes. The study was conducted on ten immature crossbred female pigs. The brain peptides were extracted from the hypothalamus (medial basal hypothalamus, preoptic area, lateral hypothalamic area, mammillary bodies, and the stalk median eminence), and pituitary gland (adenohypophysis and neurohypophysis) sections and from the ovaries and adrenal and thyroid glands. There was no difference in health status between LPS and the control groups during the period of the experiment. Nevertheless, even a low single dose of LPS from S. Enteritidis that did not result in any clinical symptoms of disease induced dysregulation of various brain peptides, such as CRH, GnRH, TRH, GAL, NPY, SOM, SP, and VIP in selected brain sections of hypothalamus, pituitary gland and in the endocrine glands of the HPA, HPO, and HPT axes. In conclusion, the obtained results clearly show that subclinical LPS from S. Enteritidis can affect the brain chemistry structure and dysregulate bioactive substance from selected brain sections and glands of the neuroendocrine axes. The exact mechanisms by which LPS can influence major neuroendocrine axes are not fully understood and require further studies.

ACS Style

Anita Mikołajczyk; Dagmara Złotkowska. Subclinical Lipopolysaccharide from Salmonella Enteritidis Induces Dysregulation of Bioactive Substances from Selected Brain Sections and Glands of Neuroendocrine Axes. Toxins 2019, 11, 91 .

AMA Style

Anita Mikołajczyk, Dagmara Złotkowska. Subclinical Lipopolysaccharide from Salmonella Enteritidis Induces Dysregulation of Bioactive Substances from Selected Brain Sections and Glands of Neuroendocrine Axes. Toxins. 2019; 11 (2):91.

Chicago/Turabian Style

Anita Mikołajczyk; Dagmara Złotkowska. 2019. "Subclinical Lipopolysaccharide from Salmonella Enteritidis Induces Dysregulation of Bioactive Substances from Selected Brain Sections and Glands of Neuroendocrine Axes." Toxins 11, no. 2: 91.

Journal article
Published: 01 January 2019 in Journal of Dairy Science
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We investigated the yogurt starter cultures of Lactobacillus bulgaricus 151 and Streptococcus thermophilus MK-10 for their effect on the severity of experimental colitis, lymphocyte profile, and regulatory T cell response. Colitis was induced in BALB/c mice via the administration of 3.5% dextran sulfate sodium salt (DSS) in drinking water for 6 d. Next, the mice were gavaged intragastrically with an active yogurt cultures (YC) mixture (~5 × 10 cfu/mouse per day) or saline (vehicle) for 8 d. Mice receiving DSS or saline alone served as positive and negative controls, respectively. The length of the colon, disease activity index, histological scores, myeloperoxidase activity, epithelium-associated microbes, short-chain fatty acid profile, total IgA antibody-forming cells, CD3CD8, CD3CD4, CD3CD4CD25, CD3CD4CD25Foxp3 T-cell subsets, and cytokine profiles (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and tumor necrosis factor) were examined after termination of the mice. Feeding mice with YC mixture reduced disease symptoms and modified intestinal microbiota and host inflammatory responsiveness to DSS. We observed limited weight loss and a decreased disease activity index score, lowered myeloperoxidase activity, and somewhat reduced damage of the intestine. The YC mixture upregulated the colon length, increased the amount and diversity of mucosa-associated microbes (enterobacteria, enterococci, and yeast), and decreased the concentration of putrefactive short-chain fatty acids in the cecal contents. It downregulated the input of cytotoxic CD3CD8 T cells and CD3CD4CD25FoxP3 regulatory T cells in Peyer's patches and enhanced CD3CD4CD25 T cells in spleens and CD3CD4CD25FoxP3 cells in peripheral blood mononuclear cells. Simultaneously, IgA antibody-forming cells were downregulated in mesenteric lymph nodes (MLN) and enhanced in spleens (SPL). The cultures mostly enhanced the production of cytokines tested in MLN and SPL, except for IL-6, which was downregulated in MLN. Interleukin-2 and IL-4 were the most upregulated in MLN, whereas IL-10, IL-4, IL-2, IFN-γ, and tumor necrosis factor were most upregulated in SPL. In serum, the YC mixture downregulated IFN-γ and clearly increased IL-2. Based on these results, we recognize the high anti-inflammatory and immunomodulatory potential of the L. bulgaricus 151 and S. thermophilus MK-10 set. The strains possess the ability to modulate the intestinal mucosal and systemic immune system toward both IgA production and induction of regulatory T cells, shifting Th1/Th2 balance.

ACS Style

E. Wasilewska; D. Zlotkowska; B. Wroblewska. Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis. Journal of Dairy Science 2019, 102, 37 -53.

AMA Style

E. Wasilewska, D. Zlotkowska, B. Wroblewska. Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis. Journal of Dairy Science. 2019; 102 (1):37-53.

Chicago/Turabian Style

E. Wasilewska; D. Zlotkowska; B. Wroblewska. 2019. "Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis." Journal of Dairy Science 102, no. 1: 37-53.

Journal article
Published: 01 December 2018 in Journal of Dairy Science
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The aim of this study was to evaluate the ex vivo and in vivo studies immune potential of α- and κ-casein. Ex vivo, naïve mouse splenocytes were stimulated with α- or κ-casein. After 120 h of culture, the proliferation index (PI), determined by 3-(4,5 dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and carboxyfluorescein diacetate N-succinimidyl ester (CFSE) staining, did not vary for either antigen, suggesting similar ex vivo immunogenic potential of both casein fractions. In vivo, BALB/ccmdb mice were sensitized with α- or κ-casein and then gavaged with primary antigen. Mice immunized with α-casein had higher levels of IgG (2) and IgA (2) in serum at the end of the experiment compared with mice immunized with κ-casein (2 and 2 for IgG and IgA, respectively). The use of α-casein for mouse immunization and ex vivo lymphocyte stimulation resulted in higher concentrations of secreted cytokines (IL4, IL10) compared with κ-casein stimulation. This is consistent with increasing regulatory T cell (Treg) lymphocyte populations, independent of the antigen used for stimulation. In summary, the immunogenic potential of α- and κ-casein was similar. Humoral and cellular immune responses confirmed their strong, independent potential to induce B and T cells. We propose that the lymphocyte proliferation index be used as an initial screening for protein immunogenicity.

ACS Style

Ewa Fuc; Dagmara Złotkowska; Emilia Stachurska; Barbara Wróblewska. Immunoreactive properties of α-casein and κ-casein: Ex vivo and in vivo studies. Journal of Dairy Science 2018, 101, 10703 -10713.

AMA Style

Ewa Fuc, Dagmara Złotkowska, Emilia Stachurska, Barbara Wróblewska. Immunoreactive properties of α-casein and κ-casein: Ex vivo and in vivo studies. Journal of Dairy Science. 2018; 101 (12):10703-10713.

Chicago/Turabian Style

Ewa Fuc; Dagmara Złotkowska; Emilia Stachurska; Barbara Wróblewska. 2018. "Immunoreactive properties of α-casein and κ-casein: Ex vivo and in vivo studies." Journal of Dairy Science 101, no. 12: 10703-10713.

Journal article
Published: 22 October 2018 in International Journal of Molecular Sciences
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Mounting evidence has indicated that lipopolysaccharide (LPS) is implicated in neuroimmunological responses, but the body’s response to subclinical doses of bacterial endotoxin remains poorly understood. The influence of a low single dose of LPS from Salmonella Enteritidis, which does not result in any clinical symptoms of intoxication (subclinical lipopolysaccharide), on selected cells and signal molecules of the neuroimmune system was tested. Five juvenile crossbred female pigs were intravenously injected with LPS from S. Enteritidis (5 μg/kg body weight (b.w.)), while five pigs from the control group received sodium chloride in the same way. Our data demonstrated that subclinical LPS from S. Enteritidis increased levels of dopamine in the brain and neuropeptides such as substance P (SP), galanin (GAL), neuropeptide Y (NPY), and active intestinal peptide (VIP) in the cervical lymph nodes with serum hyperhaptoglobinaemia and reduction of plasma CD4 and CD8 T-lymphocytes seven days after lipopolysaccharide administration. CD4 and CD8 T-lymphocytes from the cervical lymph node and serum interleukin-6 and tumour necrosis factor α showed no significant differences between the control and lipopolysaccharide groups. Subclinical lipopolysaccharide from S. Enteritidis can affect cells and signal molecules of the neuroimmune system. The presence of subclinical lipopolysaccharide from S. Enteritidis is associated with unknown prolonged consequences and may require eradication and a deeper search into the asymptomatic carrier state of Salmonella spp.

ACS Style

Anita Mikołajczyk; Dagmara Złotkowska. Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis. International Journal of Molecular Sciences 2018, 19, 3274 .

AMA Style

Anita Mikołajczyk, Dagmara Złotkowska. Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis. International Journal of Molecular Sciences. 2018; 19 (10):3274.

Chicago/Turabian Style

Anita Mikołajczyk; Dagmara Złotkowska. 2018. "Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis." International Journal of Molecular Sciences 19, no. 10: 3274.

Journals
Published: 15 January 2018 in Food & Function
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The objective of this work was to identify the nutritional and physiological effects of commercial soy and whey protein preparations.

ACS Style

Barbara Wroblewska; Jerzy Juskiewicz; Bartosz Kroplewski; Adam Jurgonski; Ewa Wasilewska; Dagmara Złotkowska; Lidia Hanna Markiewicz. The effects of whey and soy proteins on growth performance, gastrointestinal digestion, and selected physiological responses in rats. Food & Function 2018, 9, 1500 -1509.

AMA Style

Barbara Wroblewska, Jerzy Juskiewicz, Bartosz Kroplewski, Adam Jurgonski, Ewa Wasilewska, Dagmara Złotkowska, Lidia Hanna Markiewicz. The effects of whey and soy proteins on growth performance, gastrointestinal digestion, and selected physiological responses in rats. Food & Function. 2018; 9 (3):1500-1509.

Chicago/Turabian Style

Barbara Wroblewska; Jerzy Juskiewicz; Bartosz Kroplewski; Adam Jurgonski; Ewa Wasilewska; Dagmara Złotkowska; Lidia Hanna Markiewicz. 2018. "The effects of whey and soy proteins on growth performance, gastrointestinal digestion, and selected physiological responses in rats." Food & Function 9, no. 3: 1500-1509.

Journal article
Published: 12 December 2017 in Gut Pathogens
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The present research was conducted to investigate the influence of a low, single dose of LPS, which does not result in any clinical symptoms of intoxication on the expression of selected neuropeptides within the intestines of the domestic pig. This experiment was conducted on immature female pigs of the Pitrain × Duroc breed (n = five per group). Seven days after the intravenous injection of 10 mL saline solution for control animals and 5 μg/kg b.w. (in 10 mL saline solution) LPS Salmonella Enteritidis for the experimental group, the excised segments of duodenum, jejunum, ileum, ileocecal valve, caecum, descending colon, transverse colon, ascending colon and rectum were prepared to extract the main enteric neuropeptides, including GAL, NPY, SOM, SP, VIP. The results of this research indicate that single low-dose LPS S. Enteritidis produced changes in the content of the selected neuropeptides of the porcine intestine. The most visible changes were observed in the transverse colon, where LPS induced the increase of GAL expression from 19.41 ± 7.121 to 92.92 ± 11.447 ng/g tissue. The exact functions of the substances studied and mechanisms of responses to LPS action depend on the sections of the intestines. The mechanisms of observed changes are not fully understood, but fluctuations in neuronal active substance levels may be connected with neurodegenerative and/or pro-inflammatory activity of LPS.

ACS Style

Anita Mikołajczyk; Sławomir Gonkowski; Dagmara Złotkowska. Modulation of the main porcine enteric neuropeptides by a single low-dose of lipopolysaccharide (LPS) Salmonella Enteritidis. Gut Pathogens 2017, 9, 73 .

AMA Style

Anita Mikołajczyk, Sławomir Gonkowski, Dagmara Złotkowska. Modulation of the main porcine enteric neuropeptides by a single low-dose of lipopolysaccharide (LPS) Salmonella Enteritidis. Gut Pathogens. 2017; 9 (1):73.

Chicago/Turabian Style

Anita Mikołajczyk; Sławomir Gonkowski; Dagmara Złotkowska. 2017. "Modulation of the main porcine enteric neuropeptides by a single low-dose of lipopolysaccharide (LPS) Salmonella Enteritidis." Gut Pathogens 9, no. 1: 73.

Journals
Published: 15 March 2017 in Food & Function
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Obesity is a serious public health problem and being multifactorial is difficult to tackle.

ACS Style

D. Świątecka; D. Złotkowska; L. H. Markiewicz; A. M. Szyc; Barbara Wróblewska. Impact of whey proteins on the systemic and local intestinal level of mice with diet induced obesity. Food & Function 2017, 8, 1708 -1717.

AMA Style

D. Świątecka, D. Złotkowska, L. H. Markiewicz, A. M. Szyc, Barbara Wróblewska. Impact of whey proteins on the systemic and local intestinal level of mice with diet induced obesity. Food & Function. 2017; 8 (4):1708-1717.

Chicago/Turabian Style

D. Świątecka; D. Złotkowska; L. H. Markiewicz; A. M. Szyc; Barbara Wróblewska. 2017. "Impact of whey proteins on the systemic and local intestinal level of mice with diet induced obesity." Food & Function 8, no. 4: 1708-1717.

Journal article
Published: 01 September 2014 in Journal of Biotechnology
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Justyna Chudzik Kozlowska; Dagmara Zlotkowska. Immune response in Balb/C vs C57BL/6 mice during oral immunization with pea albumins (Pisum sativum). Journal of Biotechnology 2014, 185, S77 .

AMA Style

Justyna Chudzik Kozlowska, Dagmara Zlotkowska. Immune response in Balb/C vs C57BL/6 mice during oral immunization with pea albumins (Pisum sativum). Journal of Biotechnology. 2014; 185 ():S77.

Chicago/Turabian Style

Justyna Chudzik Kozlowska; Dagmara Zlotkowska. 2014. "Immune response in Balb/C vs C57BL/6 mice during oral immunization with pea albumins (Pisum sativum)." Journal of Biotechnology 185, no. : S77.

Journal article
Published: 01 September 2014 in Journal of Biotechnology
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Dagmara Zlotkowska; Justyna Chudzik Kozlowska; Ewa Wasilewska. Modulation of T cell response by probiotics orally delivered to Balb/C vs C57BL/6 mice. Journal of Biotechnology 2014, 185, S77 .

AMA Style

Dagmara Zlotkowska, Justyna Chudzik Kozlowska, Ewa Wasilewska. Modulation of T cell response by probiotics orally delivered to Balb/C vs C57BL/6 mice. Journal of Biotechnology. 2014; 185 ():S77.

Chicago/Turabian Style

Dagmara Zlotkowska; Justyna Chudzik Kozlowska; Ewa Wasilewska. 2014. "Modulation of T cell response by probiotics orally delivered to Balb/C vs C57BL/6 mice." Journal of Biotechnology 185, no. : S77.

Review
Published: 09 August 2013 in Postępy Higieny i Medycyny Doświadczalnej
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The gut microbiota comprises a large and diverse range of microorganisms whose activities have a significant impact on health. It interacts with its host at both the local and systemic level, resulting in a broad range of beneficial or detrimental outcomes for nutrition, infections, xenobiotic metabolism, and cancer. The current paper reviews research on the role of intestinal microflora in colorectal cancer development. Especially a protective effect of beneficial bacteria and probiotics on the risk of cancer development is highly discussed. There is substantial experimental evidence that the beneficial gut bacteria and their metabolism have the potential to inhibit the development and progression of neoplasia in the large intestine. Most of the data derive, however, from experimental and animal trials. Over a dozen well-documented animal studies have been published, wherein it has been clearly revealed that some lactic acid bacteria, especially lactobacilli and bifidobacteria, inhibit initiation and progression of colorectal cancer. Studies on cancer suppression in humans as a result of the consumption of probiotics are still sparse. Nevertheless, some epidemiological and interventional studies seem to confirm the bacterial anticancerogenic activity also in human gut. The mechanism by which probiotics may inhibit cancer development is unknown. Probiotics increase the amount of beneficial bacteria and decrease the pathogen level in the gut, consequently altering metabolic, enzymatic and carcinogenic activity in the intestine, decreasing inflammation and enhancing immune function, which may contribute to cancer defense.

ACS Style

Ewa Wasilewska; Dagmara Złotkowska; Mariola E. Pijagin. [The role of intestinal microflora and probiotic bacteria in prophylactic and development of colorectal cancer]. Postępy Higieny i Medycyny Doświadczalnej 2013, 67, 837 -847.

AMA Style

Ewa Wasilewska, Dagmara Złotkowska, Mariola E. Pijagin. [The role of intestinal microflora and probiotic bacteria in prophylactic and development of colorectal cancer]. Postępy Higieny i Medycyny Doświadczalnej. 2013; 67 ():837-847.

Chicago/Turabian Style

Ewa Wasilewska; Dagmara Złotkowska; Mariola E. Pijagin. 2013. "[The role of intestinal microflora and probiotic bacteria in prophylactic and development of colorectal cancer]." Postępy Higieny i Medycyny Doświadczalnej 67, no. : 837-847.

Journal article
Published: 01 January 2013 in Central European Journal of Immunology
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The aim of our study was to determine the dose and immunization scheme for commercial albumin from chicken egg white (OVA), based on immune response of mice gut mucosa. Balb/C mice were immunized orally in groups as follows: group I on day 0, 7, and 14 – 2 mg of OVA, group II on day 0, 7 and...

ACS Style

Justyna Patrycja Chudzik-Kozłowska; Dagmara Złotkowska; Henryk Kostyra. BALB/C mice as a model for immunogenicity testing of food proteins on the example of egg ovalbumin. Central European Journal of Immunology 2013, 4, 430 -433.

AMA Style

Justyna Patrycja Chudzik-Kozłowska, Dagmara Złotkowska, Henryk Kostyra. BALB/C mice as a model for immunogenicity testing of food proteins on the example of egg ovalbumin. Central European Journal of Immunology. 2013; 4 ():430-433.

Chicago/Turabian Style

Justyna Patrycja Chudzik-Kozłowska; Dagmara Złotkowska; Henryk Kostyra. 2013. "BALB/C mice as a model for immunogenicity testing of food proteins on the example of egg ovalbumin." Central European Journal of Immunology 4, no. : 430-433.

Research article
Published: 30 April 2012 in PLOS ONE
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Ovalbumin (OVA) genetically fused to protein sigma 1 (pσ1) results in tolerance to both OVA and pσ1. Pσ1 binds in a multi-step fashion, involving both protein- and carbohydrate-based receptors. To assess the relative pσ1 components responsible for inducing tolerance and the importance of its sialic binding domain (SABD) for immunization, modified OVA-pσ1, termed OVA-pσ1(short), was deleted of its SABD, but with its M cell targeting moiety intact, and was found to be immunostimulatory and enhanced CD4+ and CD8+ T cell proliferation. When used to nasally immunize mice given with and without cholera toxin (CT) adjuvant, elevated SIgA and serum IgG responses were induced, and OVA-pσ1(s) was more efficient for immunization than native OVA+CT. The immune antibodies (Abs) were derived from elevated Ab-forming cells in the upper respiratory tissues and submaxillary glands and were supported by mixed Th cell responses. Thus, these studies show that pσ1(s) can be fused to vaccines to effectively elicit improved SIgA responses.

ACS Style

Dagmara Złotkowska; Massimo Maddaloni; Carol Riccardi; Nancy Walters; Kathryn Holderness; Gayle Callis; Agnieszka Rynda-Apple; David W. Pascual. Loss of Sialic Acid Binding Domain Redirects Protein σ1 to Enhance M Cell-Directed Vaccination. PLOS ONE 2012, 7, e36182 .

AMA Style

Dagmara Złotkowska, Massimo Maddaloni, Carol Riccardi, Nancy Walters, Kathryn Holderness, Gayle Callis, Agnieszka Rynda-Apple, David W. Pascual. Loss of Sialic Acid Binding Domain Redirects Protein σ1 to Enhance M Cell-Directed Vaccination. PLOS ONE. 2012; 7 (4):e36182.

Chicago/Turabian Style

Dagmara Złotkowska; Massimo Maddaloni; Carol Riccardi; Nancy Walters; Kathryn Holderness; Gayle Callis; Agnieszka Rynda-Apple; David W. Pascual. 2012. "Loss of Sialic Acid Binding Domain Redirects Protein σ1 to Enhance M Cell-Directed Vaccination." PLOS ONE 7, no. 4: e36182.

Journal article
Published: 30 April 2012 in Journal of Neuroimmunology
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To assess the potency of regulatory T (Treg) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25+ and CD25−CD4+ T cells were adoptively transferred to naive mice, and Treg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike Treg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, Treg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag.

ACS Style

SangMu Jun; Javier Ochoa-Re; Dagmara Złotkowska; Teri Hoyt; David W. Pascual. Bystander-mediated stimulation of proteolipid protein-specific regulatory T (Treg) cells confers protection against experimental autoimmune encephalomyelitis (EAE) via TGF-β. Journal of Neuroimmunology 2012, 245, 39 -47.

AMA Style

SangMu Jun, Javier Ochoa-Re, Dagmara Złotkowska, Teri Hoyt, David W. Pascual. Bystander-mediated stimulation of proteolipid protein-specific regulatory T (Treg) cells confers protection against experimental autoimmune encephalomyelitis (EAE) via TGF-β. Journal of Neuroimmunology. 2012; 245 (1-2):39-47.

Chicago/Turabian Style

SangMu Jun; Javier Ochoa-Re; Dagmara Złotkowska; Teri Hoyt; David W. Pascual. 2012. "Bystander-mediated stimulation of proteolipid protein-specific regulatory T (Treg) cells confers protection against experimental autoimmune encephalomyelitis (EAE) via TGF-β." Journal of Neuroimmunology 245, no. 1-2: 39-47.