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The present study aimed to evaluate the feasibility of using near-infrared hyperspectral imaging (NIR-HSI) and chemometrics for quantifying deoxynivalenol (DON) in individual wheat kernels. In total, 120 wheat kernels of severely damaged kernels, moderately damaged kernels and asymptomatic kernels (SDKs, MDKs and AKs, respectively) were collected, and the DON content in the individual wheat kernels was analyzed by HPLC-MS/MS. Partial least squares (PLS), support vector machine (SVM) and local PLS based on global PLS scores (LPLS-S) algorithms were employed for building quantification models of DON. The results showed that SDKs and MDKs might contain low or no DON, while AKs could have a high DON content. Comparing the three modeling strategies, LPLS-S using mixed spectra achieved the best performance for kernels with RMSEP of 40.25 mg/kg and RPD of 2.24, which confirmed that NIR-HSI could be a feasible method for monitoring DON in individual kernels and removing highly contaminated kernels prior to food chain entry.
Guanghui Shen; Yaoyao Cao; Xianchao Yin; Fei Dong; Jianhong Xu; Jianrong Shi; Yin-Won Lee. Rapid and nondestructive quantification of deoxynivalenol in individual wheat kernels using near-infrared hyperspectral imaging and chemometrics. Food Control 2021, 131, 108420 .
AMA StyleGuanghui Shen, Yaoyao Cao, Xianchao Yin, Fei Dong, Jianhong Xu, Jianrong Shi, Yin-Won Lee. Rapid and nondestructive quantification of deoxynivalenol in individual wheat kernels using near-infrared hyperspectral imaging and chemometrics. Food Control. 2021; 131 ():108420.
Chicago/Turabian StyleGuanghui Shen; Yaoyao Cao; Xianchao Yin; Fei Dong; Jianhong Xu; Jianrong Shi; Yin-Won Lee. 2021. "Rapid and nondestructive quantification of deoxynivalenol in individual wheat kernels using near-infrared hyperspectral imaging and chemometrics." Food Control 131, no. : 108420.
Jing Li; Qing-Qing Zhi; Jie Zhang; Xiao-Yu Yuan; Li-Hong Jia; Yu-Lin Wan; Qiu-Yun Liu; Jian-Rong Shi; Zhu-Mei He. Correction to: Synthetic antimicrobial agents inhibit aflatoxin production. Brazilian Journal of Microbiology 2021, 52, 1053 -1053.
AMA StyleJing Li, Qing-Qing Zhi, Jie Zhang, Xiao-Yu Yuan, Li-Hong Jia, Yu-Lin Wan, Qiu-Yun Liu, Jian-Rong Shi, Zhu-Mei He. Correction to: Synthetic antimicrobial agents inhibit aflatoxin production. Brazilian Journal of Microbiology. 2021; 52 (2):1053-1053.
Chicago/Turabian StyleJing Li; Qing-Qing Zhi; Jie Zhang; Xiao-Yu Yuan; Li-Hong Jia; Yu-Lin Wan; Qiu-Yun Liu; Jian-Rong Shi; Zhu-Mei He. 2021. "Correction to: Synthetic antimicrobial agents inhibit aflatoxin production." Brazilian Journal of Microbiology 52, no. 2: 1053-1053.
Several weed species are known as alternative hosts of the Fusarium graminearum species complex (FGSC), and their epidemiological significance in Fusarium head blight (FHB) has been investigated, but scant information is available regarding FGSC occurrence in weeds near Chinese wheat fields. To evaluate the potential role of gramineous weeds surrounding wheat fields in FHB, 306 FGSC isolates were obtained from 210 gramineous weed samples in 2018 in Jiangsu Province. Among them, 289 were F. asiaticum and the remainder were F. graminearum. Trichothecene genotype and mycotoxin analyses revealed that 74.3% of the F. asiaticum isolates were the 3-acetyldeoxynivalenol (3ADON) chemotype and the remainder were the nivalenol (NIV) chemotype. Additionally, 82.4% of F. graminearum isolates were the 15-acetyldeoxynivalenol (15ADON) chemotype and the remainder were the NIV chemotype. FHB severity and trichothecene analysis indicated that F. asiaticum isolates with the 3ADON chemotype were more aggressive than those with the NIV chemotype in wheat. 3ADON and NIV chemotypes of F. asiaticum isolated from weeds and wheat showed no significant differences in pathogenicity in wheat. All selected F. asiaticum isolates produced perithecia, with little difference between the 3ADON and NIV chemotypes. These results highlight the epidemiology of the FGSC isolated from weeds near wheat fields, with implications for reducing FHB inoculum in China.
Fei Dong; Yunpeng Li; Xinyuan Chen; Jirong Wu; Xiao Zhang; Shufang Wang; Guizhen Ma; Yin-Won Lee; Mduduzi P. Mokoena; Ademola O. Olaniran; Jianhong Xu; Jianrong Shi. Analysis of the Fusarium graminearum species complex from gramineous weeds near wheat fields in Jiangsu Province, China. Plant Disease 2021, 1 .
AMA StyleFei Dong, Yunpeng Li, Xinyuan Chen, Jirong Wu, Xiao Zhang, Shufang Wang, Guizhen Ma, Yin-Won Lee, Mduduzi P. Mokoena, Ademola O. Olaniran, Jianhong Xu, Jianrong Shi. Analysis of the Fusarium graminearum species complex from gramineous weeds near wheat fields in Jiangsu Province, China. Plant Disease. 2021; ():1.
Chicago/Turabian StyleFei Dong; Yunpeng Li; Xinyuan Chen; Jirong Wu; Xiao Zhang; Shufang Wang; Guizhen Ma; Yin-Won Lee; Mduduzi P. Mokoena; Ademola O. Olaniran; Jianhong Xu; Jianrong Shi. 2021. "Analysis of the Fusarium graminearum species complex from gramineous weeds near wheat fields in Jiangsu Province, China." Plant Disease , no. : 1.
Fei Dong; Yunpeng Li; Xinyuan Chen; Jirong Wu; Xiao Zhang; Shufang Wang; Guizhen Ma; Yin-Won Lee; Mduduzi P Mokoena; Ademola O Olaniran; Jianhong Xu; Jianrong Shi. Analysis of the Fusarium graminearum species complex from gramineous weeds near wheat fields in Jiangsu Province, China. 2021, 1 .
AMA StyleFei Dong, Yunpeng Li, Xinyuan Chen, Jirong Wu, Xiao Zhang, Shufang Wang, Guizhen Ma, Yin-Won Lee, Mduduzi P Mokoena, Ademola O Olaniran, Jianhong Xu, Jianrong Shi. Analysis of the Fusarium graminearum species complex from gramineous weeds near wheat fields in Jiangsu Province, China. . 2021; ():1.
Chicago/Turabian StyleFei Dong; Yunpeng Li; Xinyuan Chen; Jirong Wu; Xiao Zhang; Shufang Wang; Guizhen Ma; Yin-Won Lee; Mduduzi P Mokoena; Ademola O Olaniran; Jianhong Xu; Jianrong Shi. 2021. "Analysis of the Fusarium graminearum species complex from gramineous weeds near wheat fields in Jiangsu Province, China." , no. : 1.
Antimicrobial peptides (AMPs) are biologically active molecules that can eradicate bacteria by destroying the bacterial membrane structure, causing the bacteria to rupture. However, little is known about the extent and effect of AMPs on filamentous fungi. In this study, we synthesized small molecular polypeptides by an inexpensive heat conjugation approach and examined their effects on the growth of Aspergillus flavus and its secondary metabolism. The antimicrobial agents significantly inhibited aflatoxin production, conidiation, and sclerotia formation in A. flavus. Furthermore, we found that the expression of aflatoxin structural genes was significantly inhibited, and the intracellular reactive oxygen species (ROS) level was reduced. Additionally, the antimicrobial agents can change membrane permeability. Overall, our results demonstrated that antimicrobial agents, safe to mammalian cells, have an obvious impact on aflatoxin production, which indicated that antimicrobial agents may be adopted as a new generation of potential agents for controlling aflatoxin contamination.
Jing Li; Qing-Qing Zhi; Jie Zhang; Xiao-Yu Yuan; Li-Hong Jia; Yu-Lin Wan; Qiu-Yun Liu; Jian-Rong Shi; Zhu-Mei He. Synthetic antimicrobial agents inhibit aflatoxin production. Brazilian Journal of Microbiology 2021, 52, 821 -835.
AMA StyleJing Li, Qing-Qing Zhi, Jie Zhang, Xiao-Yu Yuan, Li-Hong Jia, Yu-Lin Wan, Qiu-Yun Liu, Jian-Rong Shi, Zhu-Mei He. Synthetic antimicrobial agents inhibit aflatoxin production. Brazilian Journal of Microbiology. 2021; 52 (2):821-835.
Chicago/Turabian StyleJing Li; Qing-Qing Zhi; Jie Zhang; Xiao-Yu Yuan; Li-Hong Jia; Yu-Lin Wan; Qiu-Yun Liu; Jian-Rong Shi; Zhu-Mei He. 2021. "Synthetic antimicrobial agents inhibit aflatoxin production." Brazilian Journal of Microbiology 52, no. 2: 821-835.
Fusarium incarnatum-equiseti species complex (FIESC) strains are generally considered moderately virulent to many agricultural crops and produce a variety of mycotoxins, which represent a serious threat to food safety and public health. The occurrence of the FIESC in agricultural crops has been reported in various climatic regions, but detailed information on the species composition and toxigenic ability is rare in China. In this study, phylogenetic analyses were performed with combined sequences of EF-1a and RPB2 of 186 Fusarium isolates obtained from rice and soybean. Twelve species were identified and 156 of the isolates were resolved within the Incarnatum clade of the FIESC. Host influenced the population composition; rice isolates belonged to 12 species, among which FIESC 16, 18, and 24 were predominant; while five species were found among soybean isolates and FIESC 1, 16, and 18 dominated. Forty-three isolates were arbitrarily selected and analyzed for their Tri gene sequences and mycotoxigenic potential. Phylogenetic results based on the combined Tri5, Tri7 and Tri13 sequences were coincident with those from housekeeping markers. Type A and B trichothecenes were the main metabolites. Diacetoxyscirpenol (DAS) was detected in all strains at varying concentrations. Nivalenol (NIV), 4-acetyl nivalenol (4ANIV), 3-acetyel deoxynivalenol (3ADON), and neosolaniol (NEO) were produced in members of FIESC 1, 3, 7, 8, 15, 16, 17, and 18. Our findings contribute valuable phylogenetic and toxigenic information necessary for the risk evaluation of mycotoxins in agricultural products.
Yunan Lu; Jianbo Qiu; Ms. Shufang Wang; Jianhong Xu; Guizhen Ma; Jianrong Shi; Zenghai Bao. Species diversity and toxigenic potential of Fusarium incarnatum-equiseti species complex isolates from rice and soybean in China. Plant Disease 2021, 1 .
AMA StyleYunan Lu, Jianbo Qiu, Ms. Shufang Wang, Jianhong Xu, Guizhen Ma, Jianrong Shi, Zenghai Bao. Species diversity and toxigenic potential of Fusarium incarnatum-equiseti species complex isolates from rice and soybean in China. Plant Disease. 2021; ():1.
Chicago/Turabian StyleYunan Lu; Jianbo Qiu; Ms. Shufang Wang; Jianhong Xu; Guizhen Ma; Jianrong Shi; Zenghai Bao. 2021. "Species diversity and toxigenic potential of Fusarium incarnatum-equiseti species complex isolates from rice and soybean in China." Plant Disease , no. : 1.
Pyruvate dehydrogenase kinase (PDK) is a mitochondrial enzyme in a variety of eukaryotes, including the plant pathogen Fusarium graminearum. This enzyme can reduce the oxidation of glucose to acetyl-coA by phosphorylation and selectively inhibits the activity of pyruvate dehydrogenase (PDH), which is a kind of pyruvate dehydrogenase complex (PDC). In this study, we investigated the F. graminearum pyruvate dehydrogenase kinase encoded by FgPDK2, which is a homologue of Neurospora crassa PDK2. The disruption of the FgPDK2 gene led to several phenotypic defects including effects on mycelial growth, conidiation, pigmentation, and pathogenicity. The mutants also showed decreased resistance to osmotic stress and cell membrane/wall-damaging agents. The FgPDK2 deletion mutant exhibited reduced virulence. All of these defects were restored by genetic complementation of the mutant with the complete FgPDK2 gene. Overall, the results demonstrated that FgPDK2 is crucial for the growth of F. graminearum and can be exploited as a potential molecular target for novel fungicides to control Fusarium head blight caused by F. graminearum. Graphical abstract
Tao Gao; Dan He; Xin Liu; Fang Ji; Jianhong Xu; Jianrong Shi. The pyruvate dehydrogenase kinase 2 (PDK2) is associated with conidiation, mycelial growth, and pathogenicity in Fusarium graminearum. Food Production, Processing and Nutrition 2020, 2, 1 -10.
AMA StyleTao Gao, Dan He, Xin Liu, Fang Ji, Jianhong Xu, Jianrong Shi. The pyruvate dehydrogenase kinase 2 (PDK2) is associated with conidiation, mycelial growth, and pathogenicity in Fusarium graminearum. Food Production, Processing and Nutrition. 2020; 2 (1):1-10.
Chicago/Turabian StyleTao Gao; Dan He; Xin Liu; Fang Ji; Jianhong Xu; Jianrong Shi. 2020. "The pyruvate dehydrogenase kinase 2 (PDK2) is associated with conidiation, mycelial growth, and pathogenicity in Fusarium graminearum." Food Production, Processing and Nutrition 2, no. 1: 1-10.
Deoxynivalenol (DON) is commonly found in wheat and wheat-derived foods, posing a threat to human health. Biodegradation is an efficient and eco-friendly measure for mycotoxin detoxification. Understanding the mechanism of DON biodegradation is hence of great importance. Herein, we report the application of metabolomics methods for the analysis of DON degradation by a bacterial consortium isolated from wheat leaves collected in Jiangsu Province. Metabolomics analysis combined with a nuclear magnetic resonance analysis revealed the main degradation product, 3-keto-DON, and a minor degradation product, 3-epi-DON. Further study illustrated that DON underwent a two-step epimerization through the intermediate 3-keto-DON. Sequencing analysis of the 16S rRNA metagenome of the microorganismal community suggested that the abundance of three bacterial genera, Achromobacter, Sphingopyxis, and Sphingomonas, substantially increased during the coculture of bacterial consortium and DON. Further investigation revealed that Devosia sp. might be responsible for the epimerization of 3-keto-DON. These findings shed light on the catabolic pathways of DON during biodegradation and illustrate the potential of using metabolomics approaches in biodegradation studies. Key Points • A bacterial consortium was isolated with good deoxynivalenol-degrading potential. • Metabolomics approaches were successfully used to interpret the degradation pathway. • A trace-amount degradation product was determined by metabolomics and NMR analysis. .
Gang Wang; Yanxia Wang; Huizi Man; Yin-Won Lee; Jianrong Shi; Jianhong Xu. Metabolomics-guided analysis reveals a two-step epimerization of deoxynivalenol catalyzed by the bacterial consortium IFSN-C1. Applied Microbiology and Biotechnology 2020, 104, 6045 -6056.
AMA StyleGang Wang, Yanxia Wang, Huizi Man, Yin-Won Lee, Jianrong Shi, Jianhong Xu. Metabolomics-guided analysis reveals a two-step epimerization of deoxynivalenol catalyzed by the bacterial consortium IFSN-C1. Applied Microbiology and Biotechnology. 2020; 104 (13):6045-6056.
Chicago/Turabian StyleGang Wang; Yanxia Wang; Huizi Man; Yin-Won Lee; Jianrong Shi; Jianhong Xu. 2020. "Metabolomics-guided analysis reveals a two-step epimerization of deoxynivalenol catalyzed by the bacterial consortium IFSN-C1." Applied Microbiology and Biotechnology 104, no. 13: 6045-6056.
Fusarium head blight (FHB), caused by the fungal pathogen Fusarium graminearum, is a destructive and widespread wheat disease. Chemical fungicides are becoming less effective at reducing the disease severity of FHB, and there is a need to find a more effective, low-cost natural product. A by-product of the pyrolysis of wheat straw is a condensate known as wheat straw vinegar, which was hypothesized to be an effective F. graminearum inhibitor in wheat. The organic and mineral compositions of wheat straw vinegar were analyzed. The results of GC–MS indicated that the major organic compounds in wheat straw vinegar are phenolics and acetic acid. The main inorganic elements in the liquid were K, Ca, S and Mg. A bio-test of wheat straw vinegar showed strong antifungal activity on F. graminearum growth and production of deoxynivalenol (DON) with an EC50 (concentration for 50% of maximal effect) value of 3.1 μl ml−1. Field tests showed that the application of wheat straw vinegar diluted 200-fold significantly decreased the wheat FHB infection rate and DON content by 66% and 69%, respectively. The control efficacy of wheat straw vinegar at a dilution of 200-fold was similar to that of typical chemical fungicide applications. The use of wheat straw vinegar may increase farmers' income by reducing the net fungicide costs. Therefore, wheat straw vinegar has high potential as a natural fungicide for the control of FHB and can reduce the dependence on synthetic fungicides.
Tao Gao; Rongjun Bian; Stephen Joseph; Sarasadat Taherymoosavi; David R.G. Mitchell; Paul Munroe; Jianhong Xu; Jianrong Shi. Wheat straw vinegar: A more cost-effective solution than chemical fungicides for sustainable wheat plant protection. Science of The Total Environment 2020, 725, 138359 .
AMA StyleTao Gao, Rongjun Bian, Stephen Joseph, Sarasadat Taherymoosavi, David R.G. Mitchell, Paul Munroe, Jianhong Xu, Jianrong Shi. Wheat straw vinegar: A more cost-effective solution than chemical fungicides for sustainable wheat plant protection. Science of The Total Environment. 2020; 725 ():138359.
Chicago/Turabian StyleTao Gao; Rongjun Bian; Stephen Joseph; Sarasadat Taherymoosavi; David R.G. Mitchell; Paul Munroe; Jianhong Xu; Jianrong Shi. 2020. "Wheat straw vinegar: A more cost-effective solution than chemical fungicides for sustainable wheat plant protection." Science of The Total Environment 725, no. : 138359.
Two thermo-responsive molecularly imprinted polymers ([email protected] and [email protected]) for the selective extraction of sterigmatocystin have been prepared on the surface of the magnetic halloysite nanotubes (MHNTs) and magnetic carbon nanotubes (MCNTs), respectively. 1, 8-dihydroxyanthraquinone, n-isopropyl acrylamide, methacrylic acid, ethylene dimethacrylate and dimethyl sulfoxide were used as the dummy template, thermo-sensitive functional monomer, co-monomer, cross-linker and porogen, respectively. The magnetic properties, adsorption properties as well as the temperature responsive behaviors of [email protected] and [email protected] were systematically studied and compared for the first time. Enough saturation magnetizations of [email protected] (9.42 emu/g) and [email protected] (10.54 emu/g) were obtained. [email protected] and [email protected] also showed controllable adsorption and release behaviors to sterigmatocystin in response to the temperature change (35 °C and 20 °C). Compared with [email protected], [email protected] had higher adsorption affinity (KL = 0.120 L/mg), higher adsorption kinetic (K2 = 0.0100 g/(mg•min)) and higher imprinting factor (5.22) to sterigmatocystin. These results indicated that [email protected] was favorable adsorbent for the selective separation of sterigmatocystin. Furthermore, the elution conditions of [email protected] were optimized by response surface methodology. Under the optimal conditions, [email protected] coupled with high performance liquid chromatography were successfully applied to the selective recognition, purification, enrichment and detection of sterigmatocystin in wheat samples. The recoveries were calculated from 88.62% to 102.9% with RSDs less than 3.5 % and limit of detection of 1.1 μg/kg. This work provided a suitable carrier for the preparation of imprinted polymers and a practical approach for highly selective recognition and determination of analytes in real samples.
Ruya Wang; Pu Wu; Yanru Cui; Meriem Fizir; Jianrong Shi; Hua He. Selective recognition and enrichment of sterigmatocystin in wheat by thermo-responsive imprinted polymer based on magnetic halloysite nanotubes. Journal of Chromatography A 2020, 1619, 460952 .
AMA StyleRuya Wang, Pu Wu, Yanru Cui, Meriem Fizir, Jianrong Shi, Hua He. Selective recognition and enrichment of sterigmatocystin in wheat by thermo-responsive imprinted polymer based on magnetic halloysite nanotubes. Journal of Chromatography A. 2020; 1619 ():460952.
Chicago/Turabian StyleRuya Wang; Pu Wu; Yanru Cui; Meriem Fizir; Jianrong Shi; Hua He. 2020. "Selective recognition and enrichment of sterigmatocystin in wheat by thermo-responsive imprinted polymer based on magnetic halloysite nanotubes." Journal of Chromatography A 1619, no. : 460952.
The objective of this study was to evaluate the potential role of gramineous weeds present near paddy fields as alternative hosts for the Fusarium graminearum species complex (FGSC) that causes Fusarium head blight (FHB) in rice. 142 weed samples were collected from 10 gramineous weed species near paddy fields from August to October 2018 in Jiangsu Province, China. Of the 145 isolates of seven Fusarium species isolated from the weed samples, F. asiaticum was the most abundant (86.9%), followed by F. fujikuroi (5.5%), F. proliferatum (2.8%), F. graminearum (2.1%), F. tricinctum (1.4%), F. acuminatum (0.7%), and F. sporotrichioides (0.7%). Genotype and mycotoxin analyses confirmed that 72.2% of F. asiaticum isolates were deoxynivalenol (DON) with 3‐acetyldeoxynivalenol (3ADON) producers, and the remainder were nivalenol (NIV) producers. Pathogenicity assays showed that both 3ADON and NIV chemotypes of F. asiaticum could cause FHB in rice, but NIV chemotypes were significantly (P < 0.05) more aggressive than 3ADON chemotypes. Three Fusarium mycotoxins, DON, NIV and zearalenone, occurred naturally at low concentrations in the weed samples. Taken together, this study provides insight into the mycotoxin production and aggressiveness of F. asiaticum isolates from gramineous weeds in China.
Fei Dong; Jianhong Xu; Xiao Zhang; Shufang Wang; Yujun Xing; Mduduzi P. Mokoena; Ademola O. Olaniran; Jianrong Shi. Gramineous weeds near paddy fields are alternative hosts for the Fusarium graminearum species complex that causes fusarium head blight in rice. Plant Pathology 2020, 69, 433 -441.
AMA StyleFei Dong, Jianhong Xu, Xiao Zhang, Shufang Wang, Yujun Xing, Mduduzi P. Mokoena, Ademola O. Olaniran, Jianrong Shi. Gramineous weeds near paddy fields are alternative hosts for the Fusarium graminearum species complex that causes fusarium head blight in rice. Plant Pathology. 2020; 69 (3):433-441.
Chicago/Turabian StyleFei Dong; Jianhong Xu; Xiao Zhang; Shufang Wang; Yujun Xing; Mduduzi P. Mokoena; Ademola O. Olaniran; Jianrong Shi. 2020. "Gramineous weeds near paddy fields are alternative hosts for the Fusarium graminearum species complex that causes fusarium head blight in rice." Plant Pathology 69, no. 3: 433-441.
Deoxynivalenol (DON), a common mycotoxin of type B trichothecene, is produced mainly by several Fusarium species. DON causes great losses in farming and poses severe safety risks to human and animal health. Thus, DON contamination in cereals and DON toxicity are of worldwide concern. In this study, we screened the bacterial consortium C20, which efficiently degraded almost 70 μg ml−1 DON within 5 days. The bacterial consortium also had the ability to degrade 15-acetyl-DON, 3-acetyl-DON, and T-2 toxin. The bacterial consortium C20 was able to degrade DON under a wide range of pH and temperature conditions. The optimal temperature and pH for DON degradation were 30°C and pH 8.0, respectively. The bacterial consortium C20 comprised of different bacterial genera, and several strains were found to significantly increase when cultured in Mineral Medium with 100 μg ml−1 DON based on the analysis of the sequences of the hypervariable V3-V4 region of the 16S rRNA gene. 3-keto-DON was confirmed as a degradation product of DON by liquid chromatography/time-of-flight/mass spectrometry (LC-TOF-MS) and nuclear magnetic resonance (NMR) analyses. The results indicated that the bacterial consortium C20 is a potential candidate for the biodegradation of DON in a safe and environmentally friendly manner.
Yanxia Wang; Gang Wang; Yijun Dai; Yu Wang; Yin-Won Lee; Jianrong Shi; Jianhong Xu. Biodegradation of Deoxynivalenol by a Novel Microbial Consortium. Frontiers in Microbiology 2020, 10, 2964 .
AMA StyleYanxia Wang, Gang Wang, Yijun Dai, Yu Wang, Yin-Won Lee, Jianrong Shi, Jianhong Xu. Biodegradation of Deoxynivalenol by a Novel Microbial Consortium. Frontiers in Microbiology. 2020; 10 ():2964.
Chicago/Turabian StyleYanxia Wang; Gang Wang; Yijun Dai; Yu Wang; Yin-Won Lee; Jianrong Shi; Jianhong Xu. 2020. "Biodegradation of Deoxynivalenol by a Novel Microbial Consortium." Frontiers in Microbiology 10, no. : 2964.
Dihydroxyacid dehydratase (DHAD), encoded by ILV3, catalyses the third step in the biosynthetic pathway of branched-chain amino acids (BCAAs), which include isoleucine (Ile), leucine (Leu), and valine (Val). Enzymes involved in BCAA biosynthesis exist in bacteria, plants, and fungi but not in mammals and are therefore attractive targets for antimicrobial or herbicide development. In this study, three paralogous ILV3 genes (FgILV3A, FgILV3B, and FgILV3C) were identified in the genome of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). Deletion of FgILV3A alone or combined with FgILV3B or FgILV3C indicated an important role for FgILV3A in BCAA biosynthesis. FgILV3A deletion mutants lost the ability to grow on medium lacking amino acids. Exogenous supplementation of 1 mM Ile and Val rescued the auxotrophy of ΔFgIlv3A, though 5 mM was required to recover the growth defects in ΔFgIlv3AB and ΔFgIlv3AC strains, indicating that FgIlv3b and FgIlv3c exhibit redundant but accessory roles with FgIlv3a in BCAA biosynthesis. The auxotrophy of ΔFgIlv3A resulted in pleiotropic defects in aerial hyphal growth, in conidial formation and germination, and in aurofusarin accumulation. In addition, the mutants showed reduced virulence and deoxynivalenol production. Overall, our study demonstrates that FgIlv3a is crucial for BCAA biosynthesis in F. graminearum and a candidate fungicide target for FHB management.
Xin Liu; Yichen Jiang; Yinghui Zhang; Mingzheng Yu; Hongjun Jiang; Jianhong Xu; Jianrong Shi. FgIlv3a is crucial in branched-chain amino acid biosynthesis, vegetative differentiation, and virulence in Fusarium graminearum. Journal of Microbiology 2019, 57, 694 -703.
AMA StyleXin Liu, Yichen Jiang, Yinghui Zhang, Mingzheng Yu, Hongjun Jiang, Jianhong Xu, Jianrong Shi. FgIlv3a is crucial in branched-chain amino acid biosynthesis, vegetative differentiation, and virulence in Fusarium graminearum. Journal of Microbiology. 2019; 57 (8):694-703.
Chicago/Turabian StyleXin Liu; Yichen Jiang; Yinghui Zhang; Mingzheng Yu; Hongjun Jiang; Jianhong Xu; Jianrong Shi. 2019. "FgIlv3a is crucial in branched-chain amino acid biosynthesis, vegetative differentiation, and virulence in Fusarium graminearum." Journal of Microbiology 57, no. 8: 694-703.
Wheat Fusarium head blight (FHB), caused by Fusarium species, is a widespread and destructive fungal disease. In addition to the substantial yield and revenue losses, diseased grains are often contaminated with Fusarium mycotoxins, making them unsuitable for human consumption or use as animal feed. As a vital food and feed ingredient in China, the quality and safety of wheat and its products have gained growing attention from consumers, producers, scientists, and policymakers. This review supplies detailed data about the occurrence of Fusarium toxins and related intoxications from the 1980s to the present. Despite the serious situation of toxin contamination in wheat, the concentration of toxins in flour is usually lower than that in raw materials, and food-poisoning incidents have been considerably reduced. Much work has been conducted on every phase of toxin production and wheat circulation by scientific researchers. Regulations for maximum contamination limits have been established in recent years and play a substantial role in ensuring the stability of the national economy and people's livelihoods.
Jianbo Qiu; Jianhong Xu; Jianrong Shi; Qiu; Xu; Shi. Fusarium Toxins in Chinese Wheat since the 1980s. Toxins 2019, 11, 248 .
AMA StyleJianbo Qiu, Jianhong Xu, Jianrong Shi, Qiu, Xu, Shi. Fusarium Toxins in Chinese Wheat since the 1980s. Toxins. 2019; 11 (5):248.
Chicago/Turabian StyleJianbo Qiu; Jianhong Xu; Jianrong Shi; Qiu; Xu; Shi. 2019. "Fusarium Toxins in Chinese Wheat since the 1980s." Toxins 11, no. 5: 248.
This work provides a rapid and simple method for the determination of trace substances in complex systems.
Qiuzheng Du; Pu Wu; Fan Hu; Geyuan Li; Jianrong Shi; Hua He. Novel molecularly imprinted polymers on metal–organic frameworks as sensors for the highly selective detection of zearalenone in wheat. New Journal of Chemistry 2019, 43, 7044 -7050.
AMA StyleQiuzheng Du, Pu Wu, Fan Hu, Geyuan Li, Jianrong Shi, Hua He. Novel molecularly imprinted polymers on metal–organic frameworks as sensors for the highly selective detection of zearalenone in wheat. New Journal of Chemistry. 2019; 43 (18):7044-7050.
Chicago/Turabian StyleQiuzheng Du; Pu Wu; Fan Hu; Geyuan Li; Jianrong Shi; Hua He. 2019. "Novel molecularly imprinted polymers on metal–organic frameworks as sensors for the highly selective detection of zearalenone in wheat." New Journal of Chemistry 43, no. 18: 7044-7050.
Zearalenone is one of the most harmful mycotoxins found in grains and there is a large demand for zearalenone substrate for research purposes. A new separation method was developed for the preparative purification of zearalenone from rice culture of Fusarium graminearum by utilizing macroporous resin column combined with high-speed counter-current chromatography. Zearalenone was adsorpted on XAD-2 resin at 25 °C, neutral pH and a feed flow of 4 BV/h, followed by dynamic desorption by 60% ethanol solution. Further purification was achieved by high-speed counter-current chromatography using an optimized biphasic solvent system. A total of 267 mg of zearalenone crystal was obtained in one single run from 4.2 g of crude extract. The purity of the final product was 98.9% and the total recovery yield of zearalenone in this study was 73.9%. This dual-step purification procedure provided an effective way to obtain the costly mycotoxin for both toxicological and detoxification studies on zearalenone.
Gang Wang; Wenhua Chen; Junqiang Hu; Ben Fan; Jianrong Shi; Jianhong Xu. Preparative isolation and purification of zearalenone from rice culture by combined use of macroporous resin column and high-speed counter-current chromatography. Journal of Chromatography B 2019, 1110-1111, 43 -50.
AMA StyleGang Wang, Wenhua Chen, Junqiang Hu, Ben Fan, Jianrong Shi, Jianhong Xu. Preparative isolation and purification of zearalenone from rice culture by combined use of macroporous resin column and high-speed counter-current chromatography. Journal of Chromatography B. 2019; 1110-1111 ():43-50.
Chicago/Turabian StyleGang Wang; Wenhua Chen; Junqiang Hu; Ben Fan; Jianrong Shi; Jianhong Xu. 2019. "Preparative isolation and purification of zearalenone from rice culture by combined use of macroporous resin column and high-speed counter-current chromatography." Journal of Chromatography B 1110-1111, no. : 43-50.
Fang Ji; Mduduzi P. Mokoena; Hongyan Zhao; Ademola O. Olaniran; Jianrong Shi. Development of an immunochromatographic strip test for the rapid detection of zearalenone in wheat from Jiangsu province, China. Toxicon 2019, 158, S67 .
AMA StyleFang Ji, Mduduzi P. Mokoena, Hongyan Zhao, Ademola O. Olaniran, Jianrong Shi. Development of an immunochromatographic strip test for the rapid detection of zearalenone in wheat from Jiangsu province, China. Toxicon. 2019; 158 ():S67.
Chicago/Turabian StyleFang Ji; Mduduzi P. Mokoena; Hongyan Zhao; Ademola O. Olaniran; Jianrong Shi. 2019. "Development of an immunochromatographic strip test for the rapid detection of zearalenone in wheat from Jiangsu province, China." Toxicon 158, no. : S67.
Jianhong Xu; Hongjie Wang; Fang Ji; Xianchao Yin; Jianrong Shi. Degradation of zearalenone by Bacillus amyloliquefaciens ZDS-1 from wheat. Toxicon 2019, 158, S6 -S7.
AMA StyleJianhong Xu, Hongjie Wang, Fang Ji, Xianchao Yin, Jianrong Shi. Degradation of zearalenone by Bacillus amyloliquefaciens ZDS-1 from wheat. Toxicon. 2019; 158 ():S6-S7.
Chicago/Turabian StyleJianhong Xu; Hongjie Wang; Fang Ji; Xianchao Yin; Jianrong Shi. 2019. "Degradation of zearalenone by Bacillus amyloliquefaciens ZDS-1 from wheat." Toxicon 158, no. : S6-S7.
Gang Wang; Mingzheng Yu; Fei Dong; Jianrong Shi; Jianhong Xu. Esterase activity inspired selection of zearalenone degrading bacteria. Toxicon 2019, 158, S60 .
AMA StyleGang Wang, Mingzheng Yu, Fei Dong, Jianrong Shi, Jianhong Xu. Esterase activity inspired selection of zearalenone degrading bacteria. Toxicon. 2019; 158 ():S60.
Chicago/Turabian StyleGang Wang; Mingzheng Yu; Fei Dong; Jianrong Shi; Jianhong Xu. 2019. "Esterase activity inspired selection of zearalenone degrading bacteria." Toxicon 158, no. : S60.
Deoxynivalenol (DON), a notorious mycotoxin mainly found in Fusarium-contaminated crops, causes great loss in livestock farming and severe safety risks to human health. Here we report the isolation of a Gram-negative bacterial strain with effective biodegrading abilities on DON and its derivatives including 3-acetyl-DON and 15-acetyl-DON. The strain was identified as Devosia insulae A16 on the basis of morphological and physiological characteristics and 16S rRNA-based phylogenetic analysis. D. insulae A16 was able to degrade 88% of 20 mg/l DON within 48 h under aerobic conditions at 35 °C and neutral pH. The major degradation product of DON and its derivatives was 3-keto-DON by the oxidation of the hydroxyl group at C-3. Both 3-acetyl-DON and 15-acetyl-DON underwent a deacetylation reaction to generate DON prior to the degradation to 3-keto-DON. The results provide the potential use of D. insulae A16 as a biodegradation agent to control DON contamination in cereals.
Gang Wang; Yanxia Wang; Fang Ji; Limei Xu; Mingzheng Yu; Jianrong Shi; Jianhong Xu. Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16. Food Chemistry 2018, 276, 436 -442.
AMA StyleGang Wang, Yanxia Wang, Fang Ji, Limei Xu, Mingzheng Yu, Jianrong Shi, Jianhong Xu. Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16. Food Chemistry. 2018; 276 ():436-442.
Chicago/Turabian StyleGang Wang; Yanxia Wang; Fang Ji; Limei Xu; Mingzheng Yu; Jianrong Shi; Jianhong Xu. 2018. "Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16." Food Chemistry 276, no. : 436-442.