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Background: vegetative diversity is based on different climate and geographical origins. In terms of beekeeping, herbal diversity is strongly correlated to the production of a wide variety of honey. Therefore, based on the existing plant diversity in each country, multiple honey varieties are produced with different health characteristics. While beekeeping potential and consumption preferences are reflected in products’ variety, this leads to an increase in the region’s economy and extensive export. In the last years, monofloral honey has gained interest from consumers and especially in the medicinal field due to the presence of phytochemicals which are directly linked to health benefits, wound healing, antioxidant, anticancer and anti-inflammatory activities. Scope and approach: this review aims to highlight the physicochemical properties, mineral profiles and antioxidant activities of selected monofloral honeys based on their botanical and geographical origin. Moreover, this review focuses on the intercorrelation between monofloral honey’s antioxidant compounds and in vitro and in vivo activities, focusing on the apoptosis and cell proliferation inhibition in various cell lines, with a final usage of honey as a potential therapeutic product in the fight towards reducing tumor growth. Key findings and conclusions: multiple studies have demonstrated that monofloral honeys have different physicochemical structures and bioactive compounds. Useful chemical markers to distinguish between monofloral honeys were evidenced, such as: 2-methoxybenzoic acid and trimethoxybenzoic acid are distinctive to Manuka honey while 4-methoxyphenylacetic acid is characteristic to Kanuka honey. Furthermore, resveratrol, epigallocatechin and pinostrobin are markers distinct to Sage honey, whereas carvacrol and thymol are found in Ziziphus honey. Due to their polyphenolic profile, monofloral honeys have significant antioxidant activity, as well as antidiabetic, antimicrobial and anticancer activities. It was demonstrated that Pine honey decreased the MDA and TBARS levels in liver, kidney, heart and brain tissues, whereas Malicia honey reduced the low-density lipoprotein level. Consumption of Clover, Acacia and Gelam honeys reduced the weight and adiposity, as well as trygliceride levels. Furthermore, the antiproliferative effect of chrysin, a natural flavone in Acacia honey, was demonstrated in human (A375) and murine (B16-F1) melanoma cell lines, whereas caffeic acid, a phenolic compound found in Kelulut honey, proves to be significant candidate in the chemoprevention of colon cancer. Based on these features, the use of hiney in the medicinal field (apitherapy), and the widespread usage of natural product consumption, is gaining interest by each year.
Rodica Mărgăoan; Erkan Topal; Ralitsa Balkanska; Banu Yücel; Titanilla Oravecz; Mihaiela Cornea-Cipcigan; Dan Vodnar. Monofloral Honeys as a Potential Source of Natural Antioxidants, Minerals and Medicine. Antioxidants 2021, 10, 1023 .
AMA StyleRodica Mărgăoan, Erkan Topal, Ralitsa Balkanska, Banu Yücel, Titanilla Oravecz, Mihaiela Cornea-Cipcigan, Dan Vodnar. Monofloral Honeys as a Potential Source of Natural Antioxidants, Minerals and Medicine. Antioxidants. 2021; 10 (7):1023.
Chicago/Turabian StyleRodica Mărgăoan; Erkan Topal; Ralitsa Balkanska; Banu Yücel; Titanilla Oravecz; Mihaiela Cornea-Cipcigan; Dan Vodnar. 2021. "Monofloral Honeys as a Potential Source of Natural Antioxidants, Minerals and Medicine." Antioxidants 10, no. 7: 1023.
This study aimed to characterize raw honeys from different geographical origins in Romania, in respect of chemical composition, microbiological examination and evaluate their probiotic potential. The physico-chemical determinations were performed in APHIS-DIA Laboratory, Cluj-Napoca, Romania, using standard validated methods. Bacterial identification was performed for each sample and each colony type using Vitek® 2 Compact 15 system and PCR amplification using 16S rDNA bacterial universal primers (27F, 1492R), species being confirm by sequences analysis. In five raw honey samples, we have identified probiotic bacteria, such as: Bacillus mycoides, Bacillus thuringiensis, Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus velezensis. Generally, all honey samples meet the standard values for chemical composition. However, one sample having 7.44% sucrose was found to have also probiotics bacteria from the genus Bacillus because sucrose is a substrate for probiotics development. In conclusion, the Romanian raw honey can be a potential reservoir of probiotics, which confer a health benefit for consumers.
Claudia Pașca; Liviu Mărghitaș; Ioana Matei; Victorița Bonta; Rodica Mărgăoan; Florina Copaciu; Otilia Bobiș; Maria Campos; Daniel Dezmirean. Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation. Applied Sciences 2021, 11, 5816 .
AMA StyleClaudia Pașca, Liviu Mărghitaș, Ioana Matei, Victorița Bonta, Rodica Mărgăoan, Florina Copaciu, Otilia Bobiș, Maria Campos, Daniel Dezmirean. Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation. Applied Sciences. 2021; 11 (13):5816.
Chicago/Turabian StyleClaudia Pașca; Liviu Mărghitaș; Ioana Matei; Victorița Bonta; Rodica Mărgăoan; Florina Copaciu; Otilia Bobiș; Maria Campos; Daniel Dezmirean. 2021. "Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation." Applied Sciences 11, no. 13: 5816.
Erkan Topal; Özgür Ceylan; Mustafa Kösoğlu; Rodica Mărgăoan; Mihaiela Cornea Cipcigan. BAL MUMUNUN YAPISI, KULLANIM ALANLARI ve BAZI TEMEL SORUNLARI. Uludağ Arıcılık Dergisi 2020, 20, 209 -220.
AMA StyleErkan Topal, Özgür Ceylan, Mustafa Kösoğlu, Rodica Mărgăoan, Mihaiela Cornea Cipcigan. BAL MUMUNUN YAPISI, KULLANIM ALANLARI ve BAZI TEMEL SORUNLARI. Uludağ Arıcılık Dergisi. 2020; 20 (2):209-220.
Chicago/Turabian StyleErkan Topal; Özgür Ceylan; Mustafa Kösoğlu; Rodica Mărgăoan; Mihaiela Cornea Cipcigan. 2020. "BAL MUMUNUN YAPISI, KULLANIM ALANLARI ve BAZI TEMEL SORUNLARI." Uludağ Arıcılık Dergisi 20, no. 2: 209-220.
Recently, an increasing interest is paid to bee products obtained as a result of the fermentation process. Some of them can be consumed directly (bee-collected pollen, honey, bee bread etc.), while others are the result of lactic and/or acid fermentation (honey vinegar and honey wine). Bee bread is the result of pollens’ lactic fermentation, whereas mead is obtained by honeys’ lactic fermentation. Moreover, as a result of honey acetic acid fermentation, honey vinegar is obtained. Sensory characteristics and aroma composition have been scarcely studied, which may depend on the starter culture and fermentation process. Along with the medicinal properties they are a vital resource for future researches as they are of particular importance in the food market. In this review, we discuss the aroma-active compounds, taste, and sensorial characteristics of fermented bee products along with the approaches that can be developed for the flavor improvement based on existing technologies. Furthermore, the beneficial effects on human health are also described, with special attention that should be attributed to finding the use of probiotics in these fermented products as health-promoting effects.
Rodica Mărgăoan; Mihaiela Cornea-Cipcigan; Erkan Topal; Mustafa Kösoğlu. Impact of Fermentation Processes on the Bioactive Profile and Health-Promoting Properties of Bee Bread, Mead and Honey Vinegar. Processes 2020, 8, 1081 .
AMA StyleRodica Mărgăoan, Mihaiela Cornea-Cipcigan, Erkan Topal, Mustafa Kösoğlu. Impact of Fermentation Processes on the Bioactive Profile and Health-Promoting Properties of Bee Bread, Mead and Honey Vinegar. Processes. 2020; 8 (9):1081.
Chicago/Turabian StyleRodica Mărgăoan; Mihaiela Cornea-Cipcigan; Erkan Topal; Mustafa Kösoğlu. 2020. "Impact of Fermentation Processes on the Bioactive Profile and Health-Promoting Properties of Bee Bread, Mead and Honey Vinegar." Processes 8, no. 9: 1081.
Cyclamen species (swinebread spp.) are perennial herbaceous plants which belong to the Primulaceae family, widely used as medicinal or ornamental plants. Currently, gibberellic acid (GA3), an environmentally friendly bioregulator is widely used to enhance the productivity and phenotypic characteristics of multiple ornamental crops. A dual experiment was conducted to evaluate the influence of gibberellic acid (50, 100 or 150 mg/L), on germination earliness of seeds, along with growth and ornamental quality of selected Cyclamen species. Additionally, a greenhouse experiment with long and short sunlight photoperiod was conducted to evaluate its importance in the development of Cyclamen plants. Our results indicated that under short sunlight exposure, seeds germinated earlier than those kept in long sunlight photoperiod. Plants treated with foliar applications of GA3 exhibited higher plant height, leaf area, number of leaves, root length and tuber development. Significant differences were found in germination percentage (GP), mean germination time (MGT) and seedling vigor index (SVI), within different GA3 supplementation and greenhouse sunlight intensity (short and long days). In conclusion, our study suggests that seedling vigor and ornamental quality can be increased by GA3 application, an economic strategy for enhancing germination and growth of selected Cyclamen species.
Mihaiela Cornea-Cipcigan; Doru Pamfil; Cristian Radu Sisea; Rodica Mărgăoan. Gibberellic Acid Can Improve Seed Germination and Ornamental Quality of Selected Cyclamen Species Grown Under Short and Long Days. Agronomy 2020, 10, 516 .
AMA StyleMihaiela Cornea-Cipcigan, Doru Pamfil, Cristian Radu Sisea, Rodica Mărgăoan. Gibberellic Acid Can Improve Seed Germination and Ornamental Quality of Selected Cyclamen Species Grown Under Short and Long Days. Agronomy. 2020; 10 (4):516.
Chicago/Turabian StyleMihaiela Cornea-Cipcigan; Doru Pamfil; Cristian Radu Sisea; Rodica Mărgăoan. 2020. "Gibberellic Acid Can Improve Seed Germination and Ornamental Quality of Selected Cyclamen Species Grown Under Short and Long Days." Agronomy 10, no. 4: 516.
Mihaiela Cornea-Cipcigan; Doru Pamfil; Cristian Sisea; Cornelia Gavriș; Maria Da Graça Ribeiro Campos; Rodica Mărgăoan. A REVIEW ON CYCLAMEN SPECIES: TRANSCRIPTION FACTORS VS. PHARMACOLOGICAL EFFECTS. Acta Poloniae Pharmaceutica - Drug Research 2019, 76, 919 -938.
AMA StyleMihaiela Cornea-Cipcigan, Doru Pamfil, Cristian Sisea, Cornelia Gavriș, Maria Da Graça Ribeiro Campos, Rodica Mărgăoan. A REVIEW ON CYCLAMEN SPECIES: TRANSCRIPTION FACTORS VS. PHARMACOLOGICAL EFFECTS. Acta Poloniae Pharmaceutica - Drug Research. 2019; 76 (6):919-938.
Chicago/Turabian StyleMihaiela Cornea-Cipcigan; Doru Pamfil; Cristian Sisea; Cornelia Gavriș; Maria Da Graça Ribeiro Campos; Rodica Mărgăoan. 2019. "A REVIEW ON CYCLAMEN SPECIES: TRANSCRIPTION FACTORS VS. PHARMACOLOGICAL EFFECTS." Acta Poloniae Pharmaceutica - Drug Research 76, no. 6: 919-938.
Bee products were historically used as a therapheutic approach and in food consumption, while more recent data include important details that could validate them as food supplements due to their bioproperties, which support their future use as medicines. In this review data, data collected from bee pollen (BP) and bee bread (BB) essays will be discussed and detailed for their nutritional and health protective properties as functional foods. Dietary antioxidants intake derived from BP and BB have been associated with the prevention and clinical treatment of multiple diseases. The beneficial effects of BP and BB on health result from the presence of multiple polyphenols which possess anti-inflammatory properties, phytosterols and fatty acids, which play anticancerogenic roles, as well as polysaccharides, which stimulate immunological activity. From the main bioactivity studies with BP and BB, in vitro studies and animal experiments, the stimulation of apoptosis and the inhibition of cell proliferation in multiple cell lines could be one of the major therapeutic adjuvant effects to be explored in reducing tumor growth. Tables summarizing the main data available in this field and information about other bio-effects of BP and BB, which support the conclusions, are provided. Additionally, a discussion about the research gaps will be presented to help further experiments that complete the tree main World Health Organization (WHO) Directives of Efficiency, Safety and Quality Control for these products.
Rodica Mărgăoan; Mirela Stranț; Alina Varadi; Erkan Topal; Banu Yücel; Mihaiela Cornea-Cipcigan; Maria G. Campos; Dan C. Vodnar. Bee Collected Pollen and Bee Bread: Bioactive Constituents and Health Benefits. Antioxidants 2019, 8, 568 .
AMA StyleRodica Mărgăoan, Mirela Stranț, Alina Varadi, Erkan Topal, Banu Yücel, Mihaiela Cornea-Cipcigan, Maria G. Campos, Dan C. Vodnar. Bee Collected Pollen and Bee Bread: Bioactive Constituents and Health Benefits. Antioxidants. 2019; 8 (12):568.
Chicago/Turabian StyleRodica Mărgăoan; Mirela Stranț; Alina Varadi; Erkan Topal; Banu Yücel; Mihaiela Cornea-Cipcigan; Maria G. Campos; Dan C. Vodnar. 2019. "Bee Collected Pollen and Bee Bread: Bioactive Constituents and Health Benefits." Antioxidants 8, no. 12: 568.
Bee bread is a product of the hive obtained from pollen collected by bees, to which they add honey, digestive enzymes and subsequently is stored in the combs. Increasing evidence suggests bee bread’s potential therapeutic benefits, including antimicrobial properties. Bee bread is characterized by a bactericidal and bacteriostatic activity. The current study was carried out to test the antimicrobial activity of bee bread extracts, in various concentrations, against the bacterial strains: Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella enterica and Pseudomonas aeruginosa. The results of this study indicate that the first two dilutions of bee bread extract, respectively 33% and 16.66%, showed higher antimicrobial activity and the other dilutions had a lower, but visible activity depending on the pathogen on which they are tested. The best antimicrobial activity was manifested on the Staphylococcus aureus strain, where all dilutions had an inhibitory effect both at 8 hours and 12 hours.
Adriana Urcan; Adriana Criste; Daniel Dezmirean; Otilia Bobiș; Liviu Mărghitaș; Rodica Mărgăoan; Alexandra Hrinca. Antimicrobial Activity of Bee Bread Extracts Against Different Bacterial Strains. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2018, 75, 85 -91.
AMA StyleAdriana Urcan, Adriana Criste, Daniel Dezmirean, Otilia Bobiș, Liviu Mărghitaș, Rodica Mărgăoan, Alexandra Hrinca. Antimicrobial Activity of Bee Bread Extracts Against Different Bacterial Strains. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2018; 75 (2):85-91.
Chicago/Turabian StyleAdriana Urcan; Adriana Criste; Daniel Dezmirean; Otilia Bobiș; Liviu Mărghitaș; Rodica Mărgăoan; Alexandra Hrinca. 2018. "Antimicrobial Activity of Bee Bread Extracts Against Different Bacterial Strains." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 75, no. 2: 85-91.
Bee Bread samples from Romania and India were analysed by microscopy and High Performance Liquid Chromatography with Diode Array Detection (HPLC/DAD) and compared with pollen from the correspondent taxa. The quantification of sugars, fructose/glucose ratio, total phenolics and flavonoids was also carried out. From the results was possible to identify Brassica and Eucalyptus samples that present similar HPLC/DAD profiles with the respective ultraviolet (UV) identification of the main compounds as Kaempferol-3-O-glycosides and Hydrocinnamic acid derivatives. The Fructose/Glucose (F/G) ratio and the total amounts of phenolics and flavonoids was in line with the prevalence of the specie identified. These coincident fingerprints gave the identification of the samples, as was previously proposed for bee pollens. This paper relates for the first time the achievement on the taxon carried out previously only for bee pollens. It was reported for the first time that this phenolic profile remains unchanged in the case of floral pollen (hand collected), bee pollen and bee bread. Despite the biochemical transformation that occurs during the fermentation of bee bread, it seems that these phenolic compounds are not affected and remain unchanged. Also, variables such as soil and climate do not seem to influence these compounds for the kind of samples under study.
Adriana Cristina Urcan; Adriana Dalila Criste; Daniel Severus Dezmirean; Rodica Mărgăoan; André Caeiro; Maria Graça Campos. Similarity of Data from Bee Bread with the Same Taxa Collected in India and Romania. Molecules 2018, 23, 2491 .
AMA StyleAdriana Cristina Urcan, Adriana Dalila Criste, Daniel Severus Dezmirean, Rodica Mărgăoan, André Caeiro, Maria Graça Campos. Similarity of Data from Bee Bread with the Same Taxa Collected in India and Romania. Molecules. 2018; 23 (10):2491.
Chicago/Turabian StyleAdriana Cristina Urcan; Adriana Dalila Criste; Daniel Severus Dezmirean; Rodica Mărgăoan; André Caeiro; Maria Graça Campos. 2018. "Similarity of Data from Bee Bread with the Same Taxa Collected in India and Romania." Molecules 23, no. 10: 2491.
Given their beneficial effects in terms of health, the natural products, especially beehive products, have drawn the attention of consumers since long time ago. In order to guarantee the quality of these products on the market, their chemical composition needs to be analyzed. Thus, this current research had as objective the establishment of quality parameters for beehive brood food derived products: apilarnil and queen bee larvae triturate. These two products were compared with royal jelly which is the basis of brood food in the first 3 days of larval stage. The carbohydrates were determined by HPLC-IR and allowed the identification of seven carbohydrate compounds, predominantly glucose, fructose and sucrose. The lipid profile was analyzed by the Soxhlet method. The total protein content was determined by the Kjeldahl method. Free amino acids were analyzed by LC-MS. A total of 31 amino acids were identified of which nine are essential amino acids for humans.Â
Rodica Margaoan; Liviu Alexandru Marghitas; Daniel Severus Dezmirean; Otilia Bobis; Victorita Bonta; Corina Catana; Adriana Urcan; Carmen Ioana Muresan; Mirela Gabriela Margin. Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2017, 74, 51 -58.
AMA StyleRodica Margaoan, Liviu Alexandru Marghitas, Daniel Severus Dezmirean, Otilia Bobis, Victorita Bonta, Corina Catana, Adriana Urcan, Carmen Ioana Muresan, Mirela Gabriela Margin. Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2017; 74 (1):51-58.
Chicago/Turabian StyleRodica Margaoan; Liviu Alexandru Marghitas; Daniel Severus Dezmirean; Otilia Bobis; Victorita Bonta; Corina Catana; Adriana Urcan; Carmen Ioana Muresan; Mirela Gabriela Margin. 2017. "Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 74, no. 1: 51-58.
Bee-pollen was used for thousands of years as functional food and medicinal plant product. Various beneficial effects were attributed to it and its consumption was increased over the years. The tests have been made on 16 pollen samples of the following families: Rosaceae, Salicaceae, Fabaceae, Tiliaceae, Asteraceae, Brassicaceae and two polifloral pollen assortment, using difuzimetric method. Among the tested bacteria, the Onobrychis viciifolia pollen has the highest sensitivity 11.86±1.79 mm while the low antibacterial activity was registered for Brassica sp. pollen 8.65±2.65 mm. As the use of antibiotic substances over a long period of time resulted in larger doses of residues in milk and representing a potential biohazard, the use of ethanol extracts from beepollen is a real alternative in the treatment of cows with various diseases.
Liviu Alexandru Marghitas; Claudia Pasca; Daniel Severus Dezmirean; Otilia Bobiș; Victorita Bonta; Rodica Margaoan; Flore Chirila; Nicodim Fit. Antibacterial Activity of Pollen Extracts on Pathogenic Microflora from Milk. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2016, 73, 261 .
AMA StyleLiviu Alexandru Marghitas, Claudia Pasca, Daniel Severus Dezmirean, Otilia Bobiș, Victorita Bonta, Rodica Margaoan, Flore Chirila, Nicodim Fit. Antibacterial Activity of Pollen Extracts on Pathogenic Microflora from Milk. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2016; 73 (2):261.
Chicago/Turabian StyleLiviu Alexandru Marghitas; Claudia Pasca; Daniel Severus Dezmirean; Otilia Bobiș; Victorita Bonta; Rodica Margaoan; Flore Chirila; Nicodim Fit. 2016. "Antibacterial Activity of Pollen Extracts on Pathogenic Microflora from Milk." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 73, no. 2: 261.
Honeybee collected pollen exhibits high nutritional and pharmaceutical benefits for the human diet and medicine. Pollen’s antioxidant, anti-ageing, anti-inflammatory, anti-atherosclerosis, and cardioprotective activity, depending on the floral origin, are well known. Recent studies proposed that pollen may also be an excellent cancer-fighting candidate, as pollen harbours high amounts of phenolic substances. In our study, Filipendula ulmaria pollen (bee collected) was methanol-water extracted and used to verify its in vitro pharmacological activities on C26 mice cancer tumour cells. Three different concentrations of the extract were tested in antitumour assays. Monitoring was done after 6, 12, 24, and 48 hours. Promising results were obtained for antiproliferative and apoptotic activity of the pollen extracts, with high efficiency for the highest concentration (1 mg/mL). For both activities, time and concentration-dependent effects were observed. Pollen extracts or bee collected pollen has a high potential as an antitumour agent for use in human medicine, because they are both rich in bioactive compounds.
Rodica Mărgăoan; Marius Zăhan; Liviu A. Mărghitaş; Daniel S. Dezmirean; Silvio Erler; Otilia Bobiş. Antiproliferative activity and apoptotic effects of Filipendula ulmaria pollen against C26 mice colon tumour cells. Journal of Apicultural Science 2016, 60, 135 -144.
AMA StyleRodica Mărgăoan, Marius Zăhan, Liviu A. Mărghitaş, Daniel S. Dezmirean, Silvio Erler, Otilia Bobiş. Antiproliferative activity and apoptotic effects of Filipendula ulmaria pollen against C26 mice colon tumour cells. Journal of Apicultural Science. 2016; 60 (1):135-144.
Chicago/Turabian StyleRodica Mărgăoan; Marius Zăhan; Liviu A. Mărghitaş; Daniel S. Dezmirean; Silvio Erler; Otilia Bobiş. 2016. "Antiproliferative activity and apoptotic effects of Filipendula ulmaria pollen against C26 mice colon tumour cells." Journal of Apicultural Science 60, no. 1: 135-144.
Royal jelly has become a high-value commercial product and the standardization of this product is required to guarantee its quality on the market. The objective of the research activity was to pursue the chemical composition of commercial samples of Royal Jelly in Romania in order to propose standardization for this product. The physico-chemical composition of commercial Royal Jelly samples was analysed by determining quality parameters like: carbohydrates, lipids, proteins, 10-hydroxy-2-decenoic acid (10-HDA) and mineral elements. Carbohydrates analysis showed values between 3.4 % and 5.87 % for fructose, 4.12 % and 7.05 % for glucose, while for sucrose the values ranged between 0.95 % and 2.56 % (determined by HPLC-RI). The lipids content ranged between 1.85 % and 6.32 % (determined by the Soxhlet method). The protein values extended from 13.10 % (RJ2) to 17.04 % (RJ10) (the total protein content was determined by the Kjeldahl method). The values for the major fatty acid in Royal Jelly, 10-HDA, ranged between 1.35 % (RJ8) and 2.03 % (RJ10) (determined by high-performance liquid chromatography). The concentration of minerals varied between 3188.70 mg/kg and 4023.39 mg/kg (the concentration of minerals was measured using flame atomic absorption spectrometry). Potassium, followed by magnesium, sodium and calcium, occurs in the highest concentrations. The commercial Royal Jelly samples analysed presented variable physico-chemical characteristics that correspond with the values given by international quality standard proposals for Royal Jelly.
Carmen Ioana Mureșan; Liviu Alexandru Mărghitaș; Daniel Severus Dezmirean; Otilia Bobiș; Victorița Bonta; Iulia Zacharias; Rodica Mărgăoan; Claudia Pasca. Quality Parameters for Commercial Royal Jelly. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2016, 73, 1 .
AMA StyleCarmen Ioana Mureșan, Liviu Alexandru Mărghitaș, Daniel Severus Dezmirean, Otilia Bobiș, Victorița Bonta, Iulia Zacharias, Rodica Mărgăoan, Claudia Pasca. Quality Parameters for Commercial Royal Jelly. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2016; 73 (1):1.
Chicago/Turabian StyleCarmen Ioana Mureșan; Liviu Alexandru Mărghitaș; Daniel Severus Dezmirean; Otilia Bobiș; Victorița Bonta; Iulia Zacharias; Rodica Mărgăoan; Claudia Pasca. 2016. "Quality Parameters for Commercial Royal Jelly." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 73, no. 1: 1.
In this study polyphenols content and antioxidant activity of melliferous plants for the following: mint (Mentha pulegium), burdock (Arctium lappa), comfrey (Symphytum officinale), plantain (Pantago lanceolata), thyme (Thymus vulgaris), sage (Salvia officinalis), marigold (Calendula officinalis), small marshmallow (Althaea officinalis), echinacea (Echinaceea angustifolia) and black popular (Populus nigra) were investigated, using two different extraction methods. High content of polyphenols and flavones were extracted from Populus nigra, with an average of both extractions 23.14 mg GAE/g and 78.07 mg QE/g flavones. Among the studied plants, Arctium lappa registered the highest antioxidant activity (0.129 mmol Trolox/mL) in alcoholic extract and Echinaceea angustifolia with a value of 0.122 mmol Trolox/mL in aqueous extract. The lowest values were recorded for the antioxidant activity of Althaea officinalis (alcoholic extract) and Arctium lappa (aqueous extract). The results show that Arctium lappa, Echinaceea angustifolia and Populus nigra can be considered melliferous plants for their high biologically active compounds potential and bee products (honey and pollen) that having the composition of these plants will have high antioxidant and antibacterial properties.
Claudia Pasca; Liviu Alexandru Mărghitaș; Otilia Bobiș; Daniel Severus Dezmirean; Rodica Mărgăoan; Carmen Ioana Mureșan. Total Content of Polyphenols and Antioxidant Activity of Different Melliferous Plants. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2016, 73, 1 .
AMA StyleClaudia Pasca, Liviu Alexandru Mărghitaș, Otilia Bobiș, Daniel Severus Dezmirean, Rodica Mărgăoan, Carmen Ioana Mureșan. Total Content of Polyphenols and Antioxidant Activity of Different Melliferous Plants. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2016; 73 (1):1.
Chicago/Turabian StyleClaudia Pasca; Liviu Alexandru Mărghitaș; Otilia Bobiș; Daniel Severus Dezmirean; Rodica Mărgăoan; Carmen Ioana Mureșan. 2016. "Total Content of Polyphenols and Antioxidant Activity of Different Melliferous Plants." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 73, no. 1: 1.
The continuous interest in medicinal plants has brought about todays modern processing and usage of different plants. Extracts obtained from the aerial parts of Agastache foeniculum, Lavandula angustifolia, Melissa officinalis and Nepeta cataria were investigated for their antioxidant activity and polyphenolic content. The extracts were prepared from plants harvested in two phenological phases of development (beginning of blooming and full bloom) and in two distinctive time points (11 a.m. and 4 p.m.). Different amounts of total phenolic content were measured (12.44–76.43 mgGAE/g dry plant) and correlated with botanical origin, phenophase and harvesting hour. Flavone/flavonol content presented higher amounts than total polyphenol content in A. foeniculum extracts (36.87 mgQE/g compared to 27.19 mgGAE/g). All plants exhibit high antioxidant activity (73.14–81.74% inhibition and 0.265–0.554 mM Trolox equivalents), measured with DPPH and FRAP method. Fingerprint of biologically active compounds highlights the potential of using these plants as value-added products, because of their content in phenolic acids and flavonoids with antioxidant activity. The quantity and profile of polyphenols was influenced by harvesting period and hour. The best moment for harvesting these plants in order to have maximum amount of bioactive compounds, was established to be in the first decade of June (beginning of blooming) and in the afternoon.
Simona Carmen Duda; Liviu Alexandru Mărghitaş; Daniel Dezmirean; Marcel Duda; Rodica Mărgăoan; Otilia Bobiş. Changes in major bioactive compounds with antioxidant activity of Agastache foeniculum, Lavandula angustifolia, Melissa officinalis and Nepeta cataria : Effect of harvest time and plant species. Industrial Crops and Products 2015, 77, 499 -507.
AMA StyleSimona Carmen Duda, Liviu Alexandru Mărghitaş, Daniel Dezmirean, Marcel Duda, Rodica Mărgăoan, Otilia Bobiş. Changes in major bioactive compounds with antioxidant activity of Agastache foeniculum, Lavandula angustifolia, Melissa officinalis and Nepeta cataria : Effect of harvest time and plant species. Industrial Crops and Products. 2015; 77 ():499-507.
Chicago/Turabian StyleSimona Carmen Duda; Liviu Alexandru Mărghitaş; Daniel Dezmirean; Marcel Duda; Rodica Mărgăoan; Otilia Bobiş. 2015. "Changes in major bioactive compounds with antioxidant activity of Agastache foeniculum, Lavandula angustifolia, Melissa officinalis and Nepeta cataria : Effect of harvest time and plant species." Industrial Crops and Products 77, no. : 499-507.
Bee pollen is consumed for api-therapeutical, nutritional and medicinal properties. These actions are attributed to its chemical composition and mostly phenolics, carotenoids, fatty acids and vitamins. For this study bee pollen was freshly harvested and immediately placed at -180C. Two different extraction solvents were used: ethanol and methanol, in three different dilutions (60, 70, 80%). 5%, 10% and 15% pollen concentration in each solvent solution were carried out to determine the most efficient variant, possessing the highest antibacterial activity, using the disc diffusion method. Stock culture of Staphylococcus aureus bacteria was grown in Nutrient Broth at 37ºC for 24h in a shaker. The inhibition zones were different, according to the extraction solvent used and also the pollen concentration. Methanol extract (70%) of 15% pollen have the biggest inhibition diameter. Negative controls (methanol and ethanol) did not show an inhibitory effect on the tested bacteria, while positive control (Streptomicine) has the highest antimicrobial activity.
Rodica Mărgăoan; Liviu Al. Mărghitaş; Daniel S. Dezmirean; Bogdan Gherman; Flore Chirilă; Iulia Zacharias; Otilia Bobiș. Antimicrobial Activity of Bee Pollen Ethanolic and Methanolic Extracts on Staphylococcus aureus Bacterial Strain. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2015, 72, 78-80 .
AMA StyleRodica Mărgăoan, Liviu Al. Mărghitaş, Daniel S. Dezmirean, Bogdan Gherman, Flore Chirilă, Iulia Zacharias, Otilia Bobiș. Antimicrobial Activity of Bee Pollen Ethanolic and Methanolic Extracts on Staphylococcus aureus Bacterial Strain. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2015; 72 (1):78-80.
Chicago/Turabian StyleRodica Mărgăoan; Liviu Al. Mărghitaş; Daniel S. Dezmirean; Bogdan Gherman; Flore Chirilă; Iulia Zacharias; Otilia Bobiș. 2015. "Antimicrobial Activity of Bee Pollen Ethanolic and Methanolic Extracts on Staphylococcus aureus Bacterial Strain." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 72, no. 1: 78-80.
Daniel S. Dezmirean; Liviu Al. Mărghitaş; Nicodim Fiţ; Flore Chirilă; Bogdan Gherman; Rodica Mărgăoan; Adriana Aurori; Otilia Bobiș. Antibacterial Effect of Heather Honey (Calluna vulgaris) against Different Microorganisms of Clinical Importance. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2015, 72, 1 .
AMA StyleDaniel S. Dezmirean, Liviu Al. Mărghitaş, Nicodim Fiţ, Flore Chirilă, Bogdan Gherman, Rodica Mărgăoan, Adriana Aurori, Otilia Bobiș. Antibacterial Effect of Heather Honey (Calluna vulgaris) against Different Microorganisms of Clinical Importance. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2015; 72 (1):1.
Chicago/Turabian StyleDaniel S. Dezmirean; Liviu Al. Mărghitaş; Nicodim Fiţ; Flore Chirilă; Bogdan Gherman; Rodica Mărgăoan; Adriana Aurori; Otilia Bobiș. 2015. "Antibacterial Effect of Heather Honey (Calluna vulgaris) against Different Microorganisms of Clinical Importance." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 72, no. 1: 1.
Stefan Stangaciu; Liviu Al. Mărghitaş; Daniel S. Dezmirean; Victoriţa Bonta; Rodica Mărgăoan; Otilia Bobiș. Quality Parameters Needed for Bee Products Used in Apitherapy. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2015, 72, 1 .
AMA StyleStefan Stangaciu, Liviu Al. Mărghitaş, Daniel S. Dezmirean, Victoriţa Bonta, Rodica Mărgăoan, Otilia Bobiș. Quality Parameters Needed for Bee Products Used in Apitherapy. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2015; 72 (1):1.
Chicago/Turabian StyleStefan Stangaciu; Liviu Al. Mărghitaş; Daniel S. Dezmirean; Victoriţa Bonta; Rodica Mărgăoan; Otilia Bobiș. 2015. "Quality Parameters Needed for Bee Products Used in Apitherapy." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 72, no. 1: 1.
Total and individual carotenoids, fatty acid composition of total lipids, and main lipid classes of 16 fresh bee-collected pollen samples from Romania were determined by high-performance liquid chromatography with photodiode array detection and capillary gas chromatography with mass detection. Analyzed samples were found rich in lutein, whereas β-criptoxanthin and β-carotene were present in a wide range of amounts correlated with predominant botanical origin of the samples. High amounts of lutein were correlated with the presence of Callendula officinalis, Taraxacum officinale and Anthylis sp. The highest amount of total lipids was found in samples where pollen from Brassica sp. was predominant. Lipid classes were dominated by polyunsaturated fatty acids. Saturated fatty acids were determined in variable amounts. Lipid and carotenoid contents present great variability, explained by the various botanical species present in the samples.
Rodica Mărgăoan; Liviu Al. Mărghitaş; Daniel S. Dezmirean; Francisc V. Dulf; Andrea Bunea; Sonia Ancuţa Socaci; Otilia Bobiş. Predominant and Secondary Pollen Botanical Origins Influence the Carotenoid and Fatty Acid Profile in Fresh Honeybee-Collected Pollen. Journal of Agricultural and Food Chemistry 2014, 62, 6306 -6316.
AMA StyleRodica Mărgăoan, Liviu Al. Mărghitaş, Daniel S. Dezmirean, Francisc V. Dulf, Andrea Bunea, Sonia Ancuţa Socaci, Otilia Bobiş. Predominant and Secondary Pollen Botanical Origins Influence the Carotenoid and Fatty Acid Profile in Fresh Honeybee-Collected Pollen. Journal of Agricultural and Food Chemistry. 2014; 62 (27):6306-6316.
Chicago/Turabian StyleRodica Mărgăoan; Liviu Al. Mărghitaş; Daniel S. Dezmirean; Francisc V. Dulf; Andrea Bunea; Sonia Ancuţa Socaci; Otilia Bobiş. 2014. "Predominant and Secondary Pollen Botanical Origins Influence the Carotenoid and Fatty Acid Profile in Fresh Honeybee-Collected Pollen." Journal of Agricultural and Food Chemistry 62, no. 27: 6306-6316.