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Some Listeria species are important human and animal pathogens that can be found in contaminated food and produce a variety of virulence factors involved in their pathogenicity. Listeria strains exhibiting multidrug resistance are known to be progressively increasing and that is why continuous monitoring is needed. Effective therapy against pathogenic Listeria requires identification of the bacterial strain involved, as well as determining its virulence factors, such as antibiotic resistance and sensitivity. The present study describes the use of liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS) to do a global shotgun proteomics characterization for pathogenic Listeria species. This method allowed the identification of a total of 2990 non-redundant peptides, representing 2727 proteins. Furthermore, 395 of the peptides correspond to proteins that play a direct role in Listeria pathogenicity; they were identified as virulence factors, toxins and anti-toxins, or associated with either antibiotics (involved in antibiotic-related compounds production or resistance) or resistance to toxic substances. The proteomic repository obtained here can be the base for further research into pathogenic Listeria species and facilitate the development of novel therapeutics for these pathogens.
Ana Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; Pilar Calo-Mata; Angeles Sánchez-Pérez; Tomás Villa. Proteomic Characterization of Antibiotic Resistance in Listeria and Production of Antimicrobial and Virulence Factors. International Journal of Molecular Sciences 2021, 22, 8141 .
AMA StyleAna Abril, Mónica Carrera, Karola Böhme, Jorge Barros-Velázquez, Pilar Calo-Mata, Angeles Sánchez-Pérez, Tomás Villa. Proteomic Characterization of Antibiotic Resistance in Listeria and Production of Antimicrobial and Virulence Factors. International Journal of Molecular Sciences. 2021; 22 (15):8141.
Chicago/Turabian StyleAna Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; Pilar Calo-Mata; Angeles Sánchez-Pérez; Tomás Villa. 2021. "Proteomic Characterization of Antibiotic Resistance in Listeria and Production of Antimicrobial and Virulence Factors." International Journal of Molecular Sciences 22, no. 15: 8141.
The present work describes LC-ESI-MS/MS MS (liquid chromatography-electrospray ionization-tandem mass spectrometry) analyses of tryptic digestion peptides from phages that infect mastitis-causing Staphylococcus aureus isolated from dairy products. A total of 1933 nonredundant peptides belonging to 1282 proteins were identified and analyzed. Among them, 79 staphylococcal peptides from phages were confirmed. These peptides belong to proteins such as phage repressors, structural phage proteins, uncharacterized phage proteins and complement inhibitors. Moreover, eighteen of the phage origin peptides found were specific to S. aureus strains. These diagnostic peptides could be useful for the identification and characterization of S. aureus strains that cause mastitis. Furthermore, a study of bacteriophage phylogeny and the relationship among the identified phage peptides and the bacteria they infect was also performed. The results show the specific peptides that are present in closely related phages and the existing links between bacteriophage phylogeny and the respective Staphylococcus spp. infected.
Ana Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; Benito Cañas; José-Luis Rama; Tomás Villa; Pilar Calo-Mata. Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis. Foods 2021, 10, 799 .
AMA StyleAna Abril, Mónica Carrera, Karola Böhme, Jorge Barros-Velázquez, Benito Cañas, José-Luis Rama, Tomás Villa, Pilar Calo-Mata. Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis. Foods. 2021; 10 (4):799.
Chicago/Turabian StyleAna Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; Benito Cañas; José-Luis Rama; Tomás Villa; Pilar Calo-Mata. 2021. "Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis." Foods 10, no. 4: 799.
In this chapter, we describe a rapid workflow for the shotgun global phosphoproteomics analysis. The strategy is based on the use of accelerated in-solution trypsin digestion under an ultrasonic field by high-intensity focused ultrasound (HIFU) coupled to titanium dioxide (TiO2) selective phosphopeptide enrichment, fractionation by strong cation exchange chromatography (SCX), and analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a high-resolution mass spectrometer (LTQ-Orbitrap XL). The strategy was optimized for the global phosphoproteome analysis of Jurkat T-cells. Using this accelerated workflow, HIFU-TiO2-SCX-LC-MS/MS, 15,367 phosphorylation sites from 13,029 different phosphopeptides belonging to 3,163 different phosphoproteins can be efficiently identified in less than 15 h.
Mónica Carrera; Benito Cañas; Daniel Lopez-Ferrer. Rapid Shotgun Analysis. Methods in Molecular Biology 2021, 2259, 259 -268.
AMA StyleMónica Carrera, Benito Cañas, Daniel Lopez-Ferrer. Rapid Shotgun Analysis. Methods in Molecular Biology. 2021; 2259 ():259-268.
Chicago/Turabian StyleMónica Carrera; Benito Cañas; Daniel Lopez-Ferrer. 2021. "Rapid Shotgun Analysis." Methods in Molecular Biology 2259, no. : 259-268.
Anisakis simplex s.s. is a parasitic nematode that causes anisakiasis in humans. L3 stage larvae, which are present in many fish species and cephalopods all over the globe, might be consumed and develop occasionally into the L4 stage but cannot reproduce. Anisakiasis is an emerging health problem and economic concern. In recent years, proteomic methods have gained greater acceptance among scientists involved in parasitology and food science. According to that, here, we present tandem mass tag (TMT)-based shotgun proteomics to define differences in proteomic composition between L3 and L4 development stages of A. simplex s.s.
Robert Stryiński; Jesús Mateos; Elżbieta Łopieńska-Biernat; Mónica Carrera. Shotgun for L3 and L4 Development Stages. Methods in Molecular Biology 2021, 2259, 59 -75.
AMA StyleRobert Stryiński, Jesús Mateos, Elżbieta Łopieńska-Biernat, Mónica Carrera. Shotgun for L3 and L4 Development Stages. Methods in Molecular Biology. 2021; 2259 ():59-75.
Chicago/Turabian StyleRobert Stryiński; Jesús Mateos; Elżbieta Łopieńska-Biernat; Mónica Carrera. 2021. "Shotgun for L3 and L4 Development Stages." Methods in Molecular Biology 2259, no. : 59-75.
A workflow for the characterization of food-derived bioactive peptides is described in this chapter. The workflow integrates two consecutive steps: a discovery phase and a protein-based bioinformatic phase. In the first step (discovery phase), a shotgun bottom-up proteomics approach is used to create a reference data set for a selected food proteome. Afterward, in a second step (bioinformatic phase), the reference proteome is subjected to several in silico protein-based bioinformatic analyses to predict and characterize potential bioactive peptides after an in silico human gastrointestinal digestion. Using this workflow, bioactive collagen peptides, antihypertensive, antimicrobial, and antitumor peptides were predicted as potential valuable bioactive peptides from seafood and marine by-products. It is concluded that the combination of the global shotgun proteomic analysis and the analysis by protein-based bioinformatics can provide a rapid strategy for the characterization of new potential food-derived bioactive peptides.
Mónica Carrera; Manuel Pazos; Santiago P. Aubourg; José M. Gallardo. Shotgun and for the Characterization of Food-Derived. Methods in Molecular Biology 2021, 2259, 215 -223.
AMA StyleMónica Carrera, Manuel Pazos, Santiago P. Aubourg, José M. Gallardo. Shotgun and for the Characterization of Food-Derived. Methods in Molecular Biology. 2021; 2259 ():215-223.
Chicago/Turabian StyleMónica Carrera; Manuel Pazos; Santiago P. Aubourg; José M. Gallardo. 2021. "Shotgun and for the Characterization of Food-Derived." Methods in Molecular Biology 2259, no. : 215-223.
In the present protocol, extracellular vesicles (EVs) released from a primary culture of human umbilical cord mesenchymal stem cells (MSCs) were isolated by ultracentrifugation processes, characterized by transmission electron microscopy (TEM) and measured by nanoparticle tracking analysis (NTA). Protein was extracted from EVs using RIPA buffer and then was assessed for integrity. The proteomic content of the total EV protein samples was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) after labeling by tandem mass tag (TMT). This combined approach allowed the development of an effective strategy to study the protein cargo from MSC-derived EVs.
Miriam Morente-López; Juan A. Fafián-Labora; Mónica Carrera; Francisco J. de Toro; Concha Gil; Jesús Mateos; María C. Arufe. Mesenchymal Stem Cell-Derived Extracellular Isolation and Their Protein Cargo Characterization. Methods in Molecular Biology 2021, 2259, 3 -12.
AMA StyleMiriam Morente-López, Juan A. Fafián-Labora, Mónica Carrera, Francisco J. de Toro, Concha Gil, Jesús Mateos, María C. Arufe. Mesenchymal Stem Cell-Derived Extracellular Isolation and Their Protein Cargo Characterization. Methods in Molecular Biology. 2021; 2259 ():3-12.
Chicago/Turabian StyleMiriam Morente-López; Juan A. Fafián-Labora; Mónica Carrera; Francisco J. de Toro; Concha Gil; Jesús Mateos; María C. Arufe. 2021. "Mesenchymal Stem Cell-Derived Extracellular Isolation and Their Protein Cargo Characterization." Methods in Molecular Biology 2259, no. : 3-12.
This review presents some applications of proteomics and selected spectroscopic methods to validate certain aspects of seafood traceability. After a general introduction to traceability and the initial applications of proteomics to authenticate traceability information, it addresses the application of proteomics to trace seafood exposure to some increasingly abundant emergent health hazards with the potential to indicate the geographic/environmental origin, such as microplastics, triclosan and human medicinal and recreational drugs. Thereafter, it shows the application of vibrational spectroscopy (Fourier-Transform Infrared Spectroscopy (FTIR) and Fourier-Transform Raman Spectroscopy (FT Raman)) and Low Field Nuclear Magnetic Resonance (LF-NMR) relaxometry to discriminate frozen fish from thawed fish and to estimate the time and temperature history of frozen fillets by monitoring protein modifications induced by processing and storage. The review concludes indicating near future trends in the application of these techniques to ensure seafood safety and traceability.
Iciar Martinez; Isabel Sánchez-Alonso; Carmen Piñeiro; Mercedes Careche; Mónica Carrera. Protein Signatures to Trace Seafood Contamination and Processing. Foods 2020, 9, 1751 .
AMA StyleIciar Martinez, Isabel Sánchez-Alonso, Carmen Piñeiro, Mercedes Careche, Mónica Carrera. Protein Signatures to Trace Seafood Contamination and Processing. Foods. 2020; 9 (12):1751.
Chicago/Turabian StyleIciar Martinez; Isabel Sánchez-Alonso; Carmen Piñeiro; Mercedes Careche; Mónica Carrera. 2020. "Protein Signatures to Trace Seafood Contamination and Processing." Foods 9, no. 12: 1751.
Organophosphate flame retardants (OPFRs) are (re-)emergent environmental pollutants increasingly being used because of the restriction of other flame retardants. The chlorinated OPFR, tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is among those of highest environmental concern, but its potential effects in the marine environment have rarely been investigated. We exposed a widely used sentinel marine mussel species, Mytilus galloprovincialis, to 10 µg L-1 of TDCPP during 28 days and studied: (i) the kinetics of bioaccumulation and elimination of the compound, (ii) the effect on two molecular biomarkers, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities, and (iii) proteomic alterations in the gills, following an isobaric labeling quantitative shotgun proteomic approach, at two exposure times (7 and 28 days). Uptake and elimination of TDCPP by mussels were very fast, and the bioconcentration factor of this compound in mussels was 147 L kgww-1, confirming that this compound is not very bioaccumulative, as predicted by its chemical properties. GST activity was not affected by TDCPP exposure, but AChE activity was inhibited by TDCPP at both 7 and 28 days of exposure. Proteomic analysis revealed subtle effects of TDCPP in mussel gills, since few proteins (less than 2% of the analysed proteome) were significantly affected by TDCPP, and effect sizes were low. The most relevant effects detected were the up-regulation of epimerase family protein SDR39U1, an enzyme that could be involved in detoxification processes, at both exposure times, and the down-regulation of receptor-type tyrosine-protein phosphatase N2-like (PTPRN2) after 7 days of exposure, which is involved in neurotransmitter secretion and might be related to the neurotoxicity described for this compound. Exposure time rather than TDCPP exposure was the most important driver of protein abundance changes, with 33% of the proteome being affected by this factor, suggesting that stress caused by laboratory conditions could be an important confounding factor that needs to be controlled in similar ecotoxicology studies. Proteomic data are available via ProteomeXchange with identifier PXD019720.
Paula Sánchez-Marín; Leticia Vidal-Liñán; Laura Emilia Fernández-González; Rosa Montes; Rosario Rodil; José Benito Quintana; Mónica Carrera; Jesús Mateos; Angel P. Diz; Ricardo Beiras. Proteomic analysis and biochemical alterations in marine mussel gills after exposure to the organophosphate flame retardant TDCPP. Aquatic Toxicology 2020, 230, 105688 .
AMA StylePaula Sánchez-Marín, Leticia Vidal-Liñán, Laura Emilia Fernández-González, Rosa Montes, Rosario Rodil, José Benito Quintana, Mónica Carrera, Jesús Mateos, Angel P. Diz, Ricardo Beiras. Proteomic analysis and biochemical alterations in marine mussel gills after exposure to the organophosphate flame retardant TDCPP. Aquatic Toxicology. 2020; 230 ():105688.
Chicago/Turabian StylePaula Sánchez-Marín; Leticia Vidal-Liñán; Laura Emilia Fernández-González; Rosa Montes; Rosario Rodil; José Benito Quintana; Mónica Carrera; Jesús Mateos; Angel P. Diz; Ricardo Beiras. 2020. "Proteomic analysis and biochemical alterations in marine mussel gills after exposure to the organophosphate flame retardant TDCPP." Aquatic Toxicology 230, no. : 105688.
Foodborne parasitoses compared with bacterial and viral-caused diseases seem to be neglected, and their unrecognition is a serious issue. Parasitic diseases transmitted by food are currently becoming more common. Constantly changing eating habits, new culinary trends, and easier access to food make foodborne parasites’ transmission effortless, and the increase in the diagnosis of foodborne parasitic diseases in noted worldwide. This work presents the applications of numerous proteomic methods into the studies on foodborne parasites and their possible use in targeted diagnostics. Potential directions for the future are also provided.
Robert Stryiński; Elżbieta Łopieńska-Biernat; Mónica Carrera. Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe. Foods 2020, 9, 1403 .
AMA StyleRobert Stryiński, Elżbieta Łopieńska-Biernat, Mónica Carrera. Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe. Foods. 2020; 9 (10):1403.
Chicago/Turabian StyleRobert Stryiński; Elżbieta Łopieńska-Biernat; Mónica Carrera. 2020. "Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe." Foods 9, no. 10: 1403.
Staphylococcus aureus constitutes a major food-borne pathogen, as well as one of the main causative agents of mastitis in dairy ruminants. This pathogen can produce a variety of extracellular toxins; these include the shock syndrome toxin 1 (TSST-1), exfoliative toxins, staphylococcal enterotoxins (SE), hemolysins, and leukocidins. S. aureus expresses many virulence proteins, involved in evading the host defenses, hence facilitating microbial colonization of the mammary glands of the animals. In addition, S. aureus exotoxins play a role in the development of both skin infections and mastitis. Indeed, if these toxins remain in dairy products for human consumption, they can cause staphylococcal food poisoning (SFP) outbreaks. As a result, there is a need for procedures to identify the presence of exotoxins in human food, and the methods used must be fast, sensitive, reliable, and accurate. It is also essential to determine the best medical therapy for human patients suffering from S. aureus infections, as well as establishing the relevant veterinary treatment for infected ruminants, to avoid economic losses in the dairy industry. This review summarizes the role of S. aureus toxins in the development of mastitis in ruminants, their negative effects in the food and dairy industries, and the different methods used for the identification of these toxins in food destined for human consumption.
Ana G. Abril; Tomas Gonzalez-Villa; Jorge Barros-Velázquez; Benito Cañas; Angeles Sánchez-Pérez; Pilar Calo-Mata; Mónica Carrera. Staphylococcus aureus Exotoxins and Their Detection in the Dairy Industry and Mastitis. Toxins 2020, 12, 537 .
AMA StyleAna G. Abril, Tomas Gonzalez-Villa, Jorge Barros-Velázquez, Benito Cañas, Angeles Sánchez-Pérez, Pilar Calo-Mata, Mónica Carrera. Staphylococcus aureus Exotoxins and Their Detection in the Dairy Industry and Mastitis. Toxins. 2020; 12 (9):537.
Chicago/Turabian StyleAna G. Abril; Tomas Gonzalez-Villa; Jorge Barros-Velázquez; Benito Cañas; Angeles Sánchez-Pérez; Pilar Calo-Mata; Mónica Carrera. 2020. "Staphylococcus aureus Exotoxins and Their Detection in the Dairy Industry and Mastitis." Toxins 12, no. 9: 537.
Seafood is considered one of the main food allergen sources by the European Food Safety Authority (EFSA). It comprises several distinct groups of edible aquatic animals, including fish and shellfish, such as crustacean and mollusks. Recently, the EFSA recognized the high risk of food allergy over the world and established the necessity of developing new methodologies for its control. Consequently, accurate, sensitive, and fast detection methods for seafood allergy control and detection in food products are highly recommended. In this work, we present a comprehensive review of the applications of the proteomics methodologies for the detection and quantification of seafood allergens. For this purpose, two consecutive proteomics strategies (discovery and targeted proteomics) that are applied to the study and control of seafood allergies are reviewed in detail. In addition, future directions and new perspectives are also provided.
Mónica Carrera; Manuel Pazos; María Gasset. Proteomics-Based Methodologies for the Detection and Quantification of Seafood Allergens. Foods 2020, 9, 1134 .
AMA StyleMónica Carrera, Manuel Pazos, María Gasset. Proteomics-Based Methodologies for the Detection and Quantification of Seafood Allergens. Foods. 2020; 9 (8):1134.
Chicago/Turabian StyleMónica Carrera; Manuel Pazos; María Gasset. 2020. "Proteomics-Based Methodologies for the Detection and Quantification of Seafood Allergens." Foods 9, no. 8: 1134.
This review presents the primary applications of various proteomic strategies to evaluate the impact of farming conditions on food quality and safety in aquaculture products. Aquaculture is a quickly growing sector that represents 47% of total fish production. Food quality, dietary management, fish welfare, the stress response, food safety, and antibiotic resistance, which are covered by this review, are among the primary topics in which proteomic techniques and strategies are being successfully applied. The review concludes by outlining future directions and potential perspectives.
Mónica Carrera; Carmen Piñeiro; Iciar Martinez. Proteomic Strategies to Evaluate the Impact of Farming Conditions on Food Quality and Safety in Aquaculture Products. Foods 2020, 9, 1050 .
AMA StyleMónica Carrera, Carmen Piñeiro, Iciar Martinez. Proteomic Strategies to Evaluate the Impact of Farming Conditions on Food Quality and Safety in Aquaculture Products. Foods. 2020; 9 (8):1050.
Chicago/Turabian StyleMónica Carrera; Carmen Piñeiro; Iciar Martinez. 2020. "Proteomic Strategies to Evaluate the Impact of Farming Conditions on Food Quality and Safety in Aquaculture Products." Foods 9, no. 8: 1050.
Seafood is considered one of the main food allergen sources by the European Food Safety Authority (EFSA). It comprises several distinct groups of edible aquatic animals including fish and shellfish such as crustacean and mollusks. Recently the EFSA recognized the high risk of food allergy over the world and established the necessity of developing new methodologies for its control. Consequently, accurate, sensitive and fast detection methods for seafood allergy control and detection in food products are highly recommendable. In this work, we present a comprehensive review of the applications of the proteomics methodologies for the detection and quantification of seafood allergens. For that, two consecutive proteomics strategies (Discovery and Targeted Proteomics) applied for the study and control of seafood allergy are reviewed in detail. In addition, future directions and new perspectives were also provided.
Mónica Carrera; Manuel Pazos; María Gasset. Proteomics-based Methodologies for the Detection and Quantification of Seafood Allergens. 2020, 1 .
AMA StyleMónica Carrera, Manuel Pazos, María Gasset. Proteomics-based Methodologies for the Detection and Quantification of Seafood Allergens. . 2020; ():1.
Chicago/Turabian StyleMónica Carrera; Manuel Pazos; María Gasset. 2020. "Proteomics-based Methodologies for the Detection and Quantification of Seafood Allergens." , no. : 1.
Ivermectin (IVM), an antiparasitic drug, has a positive effect against Anisakis simplex s.s. infection and has been used for the treatment and prevention of anisakiasis in humans. However, the molecular mechanism of action of IVM on A. simplex s.s. remains unknown. Herein, tandem mass tag (TMT) labeling and extensive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis were used to identify the effect of IVM on the proteome of A. simplex s.s. in vitro. During the study, 3433 proteins, of which 1247 had at least two protein unique peptides, were identified. Comparative proteomics analysis revealed that 59 proteins were differentially regulated (DRPs) in IVM-treated larvae, of which 14 proteins were upregulated and 38 were downregulated after 12 h of culture, but after 24 h, 12 proteins were upregulated and 22 were downregulated. The transcription level of five randomly selected DRPs was determined by real-time PCR as a supplement to the proteomic data. The functional enrichment analysis showed that most of the DRPs were involved in oxidoreductase activity, immunogenicity, protein degradation, and other biological processes. This study has, for the first time, provided comprehensive proteomics data on A. simplex s.s. response to IVM and might deliver new insight into the molecular mechanism by which IVM acts on invasive larvae of A. simplex s.s.
Iwona Polak; Elżbieta Łopieńska-Biernat; Robert Stryiński; Jesús Mateos; Mónica Carrera. Comparative Proteomics Analysis of Anisakis simplex s.s.—Evaluation of the Response of Invasive Larvae to Ivermectin. Genes 2020, 11, 710 .
AMA StyleIwona Polak, Elżbieta Łopieńska-Biernat, Robert Stryiński, Jesús Mateos, Mónica Carrera. Comparative Proteomics Analysis of Anisakis simplex s.s.—Evaluation of the Response of Invasive Larvae to Ivermectin. Genes. 2020; 11 (6):710.
Chicago/Turabian StyleIwona Polak; Elżbieta Łopieńska-Biernat; Robert Stryiński; Jesús Mateos; Mónica Carrera. 2020. "Comparative Proteomics Analysis of Anisakis simplex s.s.—Evaluation of the Response of Invasive Larvae to Ivermectin." Genes 11, no. 6: 710.
The present work focuses on LC-ESI-MS/MS (liquid chromatography-electrospray ionization-tandem mass spectrometry) analysis of phage-origin tryptic digestion peptides from mastitis-causing Streptococcus spp. isolated from milk. A total of 2,546 non-redundant peptides belonging to 1,890 proteins were identified and analyzed. Among them, 65 phage-origin peptides were determined as specific Streptococcus spp. peptides. These peptides belong to proteins such as phage repressors, phage endopeptidases, structural phage proteins, and uncharacterized phage proteins. Studies involving bacteriophage phylogeny and the relationship between phages encoding the peptides determined and the bacteria they infect were also performed. The results show how specific peptides are present in closely related phages, and a link exists between bacteriophage phylogeny and the Streptococcus spp. they infect. Moreover, the phage peptide M∗ATNLGQAYVQIM∗PSAK is unique and specific for Streptococcus agalactiae. These results revealed that diagnostic peptides, among others, could be useful for the identification and characterization of mastitis-causing Streptococcus spp., particularly peptides that belong to specific functional proteins, such as phage-origin proteins, because of their specificity to bacterial hosts.
Ana Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; Benito Cañas; Jose L. R. Rama; Tomás G. Villa; Pilar Calo-Mata. Characterization of Bacteriophage Peptides of Pathogenic Streptococcus by LC-ESI-MS/MS: Bacteriophage Phylogenomics and Their Relationship to Their Host. Frontiers in Microbiology 2020, 11, 1241 .
AMA StyleAna Abril, Mónica Carrera, Karola Böhme, Jorge Barros-Velázquez, Benito Cañas, Jose L. R. Rama, Tomás G. Villa, Pilar Calo-Mata. Characterization of Bacteriophage Peptides of Pathogenic Streptococcus by LC-ESI-MS/MS: Bacteriophage Phylogenomics and Their Relationship to Their Host. Frontiers in Microbiology. 2020; 11 ():1241.
Chicago/Turabian StyleAna Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; Benito Cañas; Jose L. R. Rama; Tomás G. Villa; Pilar Calo-Mata. 2020. "Characterization of Bacteriophage Peptides of Pathogenic Streptococcus by LC-ESI-MS/MS: Bacteriophage Phylogenomics and Their Relationship to Their Host." Frontiers in Microbiology 11, no. : 1241.
Streptococcus spp. are major mastitis pathogens present in dairy products, which produce a variety of virulence factors that are involved in streptococcal pathogenicity. These include neuraminidase, pyrogenic exotoxin, and M protein, and in addition they might produce bacteriocins and antibiotic-resistance proteins. Unjustifiable misuse of antimicrobials has led to an increase in antibiotic-resistant bacteria present in foodstuffs. Identification of the mastitis-causing bacterial strain, as well as determining its antibiotic resistance and sensitivity is crucial for effective therapy. The present work focused on the LC–ESI–MS/MS (liquid chromatography–electrospray ionization tandem mass spectrometry) analysis of tryptic digestion peptides from mastitis-causing Streptococcus spp. isolated from milk. A total of 2706 non-redundant peptides belonging to 2510 proteins was identified and analyzed. Among them, 168 peptides were determined, representing proteins that act as virulence factors, toxins, anti-toxins, provide resistance to antibiotics that are associated with the production of lantibiotic-related compounds, or play a role in the resistance to toxic substances. Protein comparisons with the NCBI database allowed the identification of 134 peptides as specific to Streptococcus spp., while two peptides (EATGNQNISPNLTISNAQLNLEDKNK and DLWC*NM*IIAAK) were found to be species-specific to Streptococcus dysgalactiae. This proteomic repository might be useful for further studies and research work, as well as for the development of new therapeutics for the mastitis-causing Streptococcus strains.
Ana G. Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; José-Luis R. Rama; Pilar Calo-Mata; Angeles Sánchez-Pérez; Tomás G. Villa. Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors in Streptococcus Species Associated with Bovine Mastitis. Could Enzybiotics Represent Novel Therapeutic Agents Against These Pathogens? Antibiotics 2020, 9, 1 .
AMA StyleAna G. Abril, Mónica Carrera, Karola Böhme, Jorge Barros-Velázquez, José-Luis R. Rama, Pilar Calo-Mata, Angeles Sánchez-Pérez, Tomás G. Villa. Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors in Streptococcus Species Associated with Bovine Mastitis. Could Enzybiotics Represent Novel Therapeutic Agents Against These Pathogens? Antibiotics. 2020; 9 (6):1.
Chicago/Turabian StyleAna G. Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; José-Luis R. Rama; Pilar Calo-Mata; Angeles Sánchez-Pérez; Tomás G. Villa. 2020. "Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors in Streptococcus Species Associated with Bovine Mastitis. Could Enzybiotics Represent Novel Therapeutic Agents Against These Pathogens?" Antibiotics 9, no. 6: 1.
Ana G. Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; José-Luis R. Rama; Pilar Calo-Mata; Angeles Sánchez-Pérez; Tomás G. Villa. Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors in Streptococcus Species Associated with Bovine Mastitis. Could Enzybiotics Represent Novel Therapeutic Agents Against These Pathogens? Antibiotics 2020, 9, 302 .
AMA StyleAna G. Abril, Mónica Carrera, Karola Böhme, Jorge Barros-Velázquez, José-Luis R. Rama, Pilar Calo-Mata, Angeles Sánchez-Pérez, Tomás G. Villa. Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors in Streptococcus Species Associated with Bovine Mastitis. Could Enzybiotics Represent Novel Therapeutic Agents Against These Pathogens? Antibiotics. 2020; 9 (6):302.
Chicago/Turabian StyleAna G. Abril; Mónica Carrera; Karola Böhme; Jorge Barros-Velázquez; José-Luis R. Rama; Pilar Calo-Mata; Angeles Sánchez-Pérez; Tomás G. Villa. 2020. "Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors in Streptococcus Species Associated with Bovine Mastitis. Could Enzybiotics Represent Novel Therapeutic Agents Against These Pathogens?" Antibiotics 9, no. 6: 302.
BACKGROUND This study was addressed to the quality loss of European hake (Merluccius merluccius) during frozen storage. Its objective was to comparatively analyse the effect of a previous high‐pressure processing (HPP) (150 MPa for 2 min) when different storage temperatures (–10, –18 and –30 °C) were employed. RESULTS Most chemical quality indices (trimethylamine, TMA; dimethylamine, DMA, formaldehyde, FA; free fatty acids, FFA) provided a marked content increase with freezing and frozen storage time, values being higher by increasing the storage temperature. Previous HPP led to an inhibitory (p<0.05) effect on the TMA, DMA, FA and FFA formation in frozen fish kept at –10 and –18 °C, the preservative effect being higher at the highest temperature tested; however, in agreement to the low damage development, no effect could not be proved on samples stored at –30 °C. Concerning lipid oxidation, peroxides formation was found low, although a slight increasing effect (p<0.05) was implied in fish corresponding to all temperatures as a result of the previous HPP; furthermore, an inhibitory effect (p<0.05) on fluorescent compounds formation (tertiary lipid oxidation) was evident after freezing and at month 9 for –10 °C samples. CONCLUSION It is concluded that a 150‐MPa high‐pressure treatment may inhibit the formation of degradative molecules such as DMA, FA, TMA and FFA during the frozen storage at –18 °C (maximum recommended) and –10 °C. However, results have indicated that lowering the storage temperature showed to be more effective than the current HPP (150 MPa for 2 min). This article is protected by copyright. All rights reserved.
Mónica Carrera; Liliana G. Fidalgo; Manuel Vázquez; Jorge A. Saraiva; Santiago P. Aubourg. Comparative effect of a previous 150‐MPa treatment on the quality loss of frozen hake stored at different temperatures. Journal of the Science of Food and Agriculture 2020, 100, 4245 -4251.
AMA StyleMónica Carrera, Liliana G. Fidalgo, Manuel Vázquez, Jorge A. Saraiva, Santiago P. Aubourg. Comparative effect of a previous 150‐MPa treatment on the quality loss of frozen hake stored at different temperatures. Journal of the Science of Food and Agriculture. 2020; 100 (11):4245-4251.
Chicago/Turabian StyleMónica Carrera; Liliana G. Fidalgo; Manuel Vázquez; Jorge A. Saraiva; Santiago P. Aubourg. 2020. "Comparative effect of a previous 150‐MPa treatment on the quality loss of frozen hake stored at different temperatures." Journal of the Science of Food and Agriculture 100, no. 11: 4245-4251.
In this chapter, an overview of the applications of proteomics to food quality and safety in the fishery and aquaculture sectors is presented. The potentiality of proteomics is highlighted with respect to different subjects concerning fish quality and safety. Fish authentication, the study of fish quality changes during storage and processing, fish allergen detection, monitoring of fish microorganisms, dietary management and fish welfare in aquaculture are the main areas where proteomics is being successful applied. Finally, future directions and potential perspectives are also discussed.
Mónica Carrera; José M. Gallardo. Chapter 14. Applications of Proteomics to Food Quality and Safety in Fisheries and Aquaculture. Food Chemistry, Function and Analysis 2020, 330 -353.
AMA StyleMónica Carrera, José M. Gallardo. Chapter 14. Applications of Proteomics to Food Quality and Safety in Fisheries and Aquaculture. Food Chemistry, Function and Analysis. 2020; ():330-353.
Chicago/Turabian StyleMónica Carrera; José M. Gallardo. 2020. "Chapter 14. Applications of Proteomics to Food Quality and Safety in Fisheries and Aquaculture." Food Chemistry, Function and Analysis , no. : 330-353.
La alergia alimentaria es el cuarto principal problema de salud pública según datos de la Organización Mundial de la Salud (OMS). Afecta a un total de 6-8% de niños y a 2-4% de adultos. Debido a la actual gran prevalencia de la alergia alimentaria, se hace necesario el desarrollo de nuevos métodos de control, tratamiento y estudio. En esta revisión se presentan los enfoques proteómicos y de biología de sistemas más recientes para el estudio de la alergia alimentaria. En este sentido, se resumen con detalle las dos principales estrategias proteómicas (proteómica de descubrimiento y proteómica dirigida). También se describen los innovadores enfoques de biología de sistemas basados en datos proteómicos para el estudio de los mecanismos de la alergia alimentaria. Finalmente se presentan nuevas perspectivas y futuras direcciones.
Mónica Carrera. Proteómica y biología de sistemas para el estudio de la alergia alimentaria. Arbor 2020, 196, 546 .
AMA StyleMónica Carrera. Proteómica y biología de sistemas para el estudio de la alergia alimentaria. Arbor. 2020; 196 (795):546.
Chicago/Turabian StyleMónica Carrera. 2020. "Proteómica y biología de sistemas para el estudio de la alergia alimentaria." Arbor 196, no. 795: 546.