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P. Sacchetti
Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Via Maragliano, Florence, Italy

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Journal article
Published: 25 June 2021 in Journal of Economic Entomology
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Three molecular protocols using qPCR TaqMan probe, SYBR Green, and loop-mediated isothermal amplification (LAMP) methods were set up for the identification of larvae and adults of an African invasive moth, Thaumatotibia leucotreta (Meyrick, 1913) (Lepidoptera: Tortricidae). The DNA extracts from larval and adult samples of T. leucotreta were perfectly amplified with an average Ct value of 19.47 ± 2.63. All assays were demonstrated to be inclusive for T. leucotreta and exclusive for the nontarget species tested; the absence of false positives for nontarget species showed a 100% of diagnostic specificity and diagnostic sensitivity for all assays. With the SYBR Green protocol, the Cq values were only considered for values less than 22 (cutoff value) to prevent false-positive results caused by the late amplification of nonspecific amplicons. The limit of detection (LoD) for the qPCR probe protocol was equal to 0.02 pg/µl while a value equal to 0.128 pg/µl for the qPCR SYBR Green assay and LAMP method were established, respectively. The intrarun variabilities of reproducibility and repeatability in all the assays evaluated as CV%, ranged between 0.21 and 6.14, and between 0.33 and 9.52, respectively; the LAMP values were slightly higher than other assays, indicating a very low interrun variability. In order for an operator to choose the most desirable method, several parameters were considered and discussed. For future development of these assays, it is possible to hypothesize the setup of a diagnostic kit including all the three methods combined, to empower the test reliability and robustness.

ACS Style

D Rizzo; D Da Lio; L Bartolini; G Cappellini; T Bruscoli; C Salemi; A Aronadio; D Del Nista; F Pennacchio; N Boersma; E Rossi; P Sacchetti. Development of Three Molecular Diagnostic Tools for the Identification of the False Codling Moth (Lepidoptera: Tortricidae). Journal of Economic Entomology 2021, 114, 1796 -1807.

AMA Style

D Rizzo, D Da Lio, L Bartolini, G Cappellini, T Bruscoli, C Salemi, A Aronadio, D Del Nista, F Pennacchio, N Boersma, E Rossi, P Sacchetti. Development of Three Molecular Diagnostic Tools for the Identification of the False Codling Moth (Lepidoptera: Tortricidae). Journal of Economic Entomology. 2021; 114 (4):1796-1807.

Chicago/Turabian Style

D Rizzo; D Da Lio; L Bartolini; G Cappellini; T Bruscoli; C Salemi; A Aronadio; D Del Nista; F Pennacchio; N Boersma; E Rossi; P Sacchetti. 2021. "Development of Three Molecular Diagnostic Tools for the Identification of the False Codling Moth (Lepidoptera: Tortricidae)." Journal of Economic Entomology 114, no. 4: 1796-1807.

Journal article
Published: 29 December 2020 in The Korean Journal of Parasitology
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In Europe, 5 Lipoptena species have been recorded, including Lipoptena fortisetosa. This species, native to Asian countries, was described as a parasite of sika deer and its appearance in Europe dates back to more than 50 years ago. Lipoptena fortisetosa has been recently reported in Italy, sharing its hosts with Lipoptena cervi. A morpho-molecular approach was developed to determine the phylogenetic interrelationship of Italian and Asian CO1 haplotypes sequenced from Lipoptena fly individuals collected in Italy, and their DNA sequences were compared with conspecifics available in GenBank; morphological key-characters (terminalia) of L. fortisetosa were compared with the original description. Two haplotypes were recorded from Italy and assigned to L. cervi and L. fortisetosa, respectively. The latter was part of the monophyletic clade L. fortisetosa, along with 2 Central European and 2 Korean haplotypes (100% identical with one of the Korean haplotypes); moreover, Italian L. fortisetosa female terminalia were consistent with the original description of Asian individuals. Pending more in-depth investigations, this study provides a first answer to the hypothesis of the recent colonization of Italy by L. fortisetosa from Asia as we did not detect any obvious and stable morphological and molecular differences in specimens from the 2 geographical areas. The presence of the sika deer in Europe was retraced and the possible route traveled by the parasite from Asia and the eco-biological factors that may have enhanced its settlement are discussed.

ACS Style

Annalisa Andreani; Annunziata Giangaspero; Marianna Marangi; Alessandra Barlaam; Maria Paola Ponzetta; Lise Roy; Antonio Belcari; Patrizia Sacchetti. Asia and Europe: So Distant So Close? The Case of Lipoptena fortisetosa in Italy. The Korean Journal of Parasitology 2020, 58, 661 -668.

AMA Style

Annalisa Andreani, Annunziata Giangaspero, Marianna Marangi, Alessandra Barlaam, Maria Paola Ponzetta, Lise Roy, Antonio Belcari, Patrizia Sacchetti. Asia and Europe: So Distant So Close? The Case of Lipoptena fortisetosa in Italy. The Korean Journal of Parasitology. 2020; 58 (6):661-668.

Chicago/Turabian Style

Annalisa Andreani; Annunziata Giangaspero; Marianna Marangi; Alessandra Barlaam; Maria Paola Ponzetta; Lise Roy; Antonio Belcari; Patrizia Sacchetti. 2020. "Asia and Europe: So Distant So Close? The Case of Lipoptena fortisetosa in Italy." The Korean Journal of Parasitology 58, no. 6: 661-668.

Encyclopedia
Published: 29 August 2020 in Reference Module in Biomedical Sciences
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Ked and bat flies (superfamily Hippoboscoidea) are ectoparasites of primary veterinary importance, for both livestock and wildlife. Besides, they may be relevant also for the public health since can attack accidentally also humans producing in some cases severe pathologies. Their peculiar morphological structures, evolved during the adaptation process, allowed them to live together with the hosts during their whole life, thanks to the presence of flattened and sclerotized body, as well as of legs provided with strongly developed claws and adhesion organs. All these species are hematophagous and both sexes feed on the victim blood with a typical trophic behaviour present in all the members of this superfamily. Due to this characteristic, these flies are involved potentially in the transmission of pathogens responsible of diseases and/or zoonoses.

ACS Style

Annalisa Andreani; Patrizia Sacchetti; Antonio Belcari. Keds and Bat Flies (Hippoboscidae, Nycteribiidae and Streblidae). Reference Module in Biomedical Sciences 2020, 1 .

AMA Style

Annalisa Andreani, Patrizia Sacchetti, Antonio Belcari. Keds and Bat Flies (Hippoboscidae, Nycteribiidae and Streblidae). Reference Module in Biomedical Sciences. 2020; ():1.

Chicago/Turabian Style

Annalisa Andreani; Patrizia Sacchetti; Antonio Belcari. 2020. "Keds and Bat Flies (Hippoboscidae, Nycteribiidae and Streblidae)." Reference Module in Biomedical Sciences , no. : 1.

Review
Published: 10 June 2020 in Insect Science
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Investigations on microbial symbioses in Tephritidae have increased over the past 30 years owing to the potential use of these relationships in developing new control strategies for economically important fruit flies. Bactrocera oleae (Rossi)—the olive fruit fly—is a monophagous species strictly associated with the olive tree, and among all the tephritids, its symbionts are the most investigated. The bacterium Candidatus Erwinia dacicola is the major persistent resident endosymbiont in wild B. oleae populations. Its relationship with B. oleae has been investigated since being identified in 2005. This endosymbiont is vertically transmitted through generations from the female to the egg. It exists at every developmental stage, although it is more abundant in larvae and ovipositing females, and is necessary for both larvae and adults. Studying B. oleae–Ca . E. dacicola, or other B. oleae –microbe interactions, will allow us to develop modern biological control systems for area‐wide olive protection and set an example for similar programs in other important food crops. This review summarizes the information available on tephritid–microbe interactions and investigates relationships among fruit flies, bacteria and host plants; however, its focus is on B. oleae and its strict association with Ca . E. dacicola to promote environmentally friendly control strategies for area‐wide pest management.

ACS Style

Gaia Bigiotti; Patrizia Sacchetti; Roberta Pastorelli; Carol R. Lauzon; Antonio Belcari. Bacterial symbiosis in Bactrocera oleae , an Achilles’ heel for its pest control. Insect Science 2020, 28, 874 -884.

AMA Style

Gaia Bigiotti, Patrizia Sacchetti, Roberta Pastorelli, Carol R. Lauzon, Antonio Belcari. Bacterial symbiosis in Bactrocera oleae , an Achilles’ heel for its pest control. Insect Science. 2020; 28 (4):874-884.

Chicago/Turabian Style

Gaia Bigiotti; Patrizia Sacchetti; Roberta Pastorelli; Carol R. Lauzon; Antonio Belcari. 2020. "Bacterial symbiosis in Bactrocera oleae , an Achilles’ heel for its pest control." Insect Science 28, no. 4: 874-884.

Original article
Published: 14 May 2020 in Medical and Veterinary Entomology
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Lipoptena cervi (Linnaeus, 1758), Lipoptena fortisetosa Maa, 1965, Hippobosca equina Linnaeus, 1758, and Pseudolynchia canariensis (Macquart, 1840) (Diptera: Hippoboscidae) are haematophagous ectoparasites that infest different mammal and bird species and occasionally attack humans. They are known for the health implications they have as vectors of pathogens to humans and animals, and for the injuries they inflict on their host's skin. This study focused on the morphological structures evolved by parasites in terms of their biology and the different environment types that they inhabit. To this aim, we examined four hippoboscid species, as well as their hosts' fur (ungulate and horse), and feather (pigeon) through light and Scanning Electron Microscopy (SEM) observations in order to highlight the main morphological features that evolved differently in these flies and to explain the effect of hosts' fur/feather microhabitats on the morphological specializations observed in the investigated ectoparasites. The studied species showed main convergent characters in mouthparts while remarkable differences have been detected on the antennal sensillar pattern as well as on the leg acropod that displayed divergent characters evolved in relation to the host.

ACS Style

A. Andreani; P. Sacchetti; A. Belcari. Evolutionary adaptations in four hippoboscid fly species belonging to three different subfamilies. Medical and Veterinary Entomology 2020, 34, 344 -363.

AMA Style

A. Andreani, P. Sacchetti, A. Belcari. Evolutionary adaptations in four hippoboscid fly species belonging to three different subfamilies. Medical and Veterinary Entomology. 2020; 34 (3):344-363.

Chicago/Turabian Style

A. Andreani; P. Sacchetti; A. Belcari. 2020. "Evolutionary adaptations in four hippoboscid fly species belonging to three different subfamilies." Medical and Veterinary Entomology 34, no. 3: 344-363.

Journal article
Published: 18 December 2019 in BMC Biotechnology
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Background The symbiosis between the olive fruit fly, Bactrocera oleae, and Candidatus Erwinia dacicola has been demonstrated as essential for the fly’s larval development and adult physiology. The mass rearing of the olive fruit fly has been hindered by several issues, including problems which could be related to the lack of the symbiont, presumably due to preservatives and antibiotics currently used during rearing under laboratory conditions. To better understand the mechanisms underlying symbiont removal or loss during the rearing of lab colonies of the olive fruit fly, we performed experiments that focused on bacterial transfer from wild female flies to their eggs. In this research, eggs laid by wild females were treated with propionic acid solution, which is often used as an antifungal agent, a mixture of sodium hypochlorite and Triton X, or water (as a control). The presence of the bacterial symbiont on eggs was evaluated by real-time PCR and scanning electron microscopy. Results DGGE analysis showed a clear band with the same migration behavior present in all DGGE profiles but with a decreasing intensity. Molecular analyses performed by real-time PCR showed a significant reduction in Ca. E. dacicola abundance in eggs treated with propionic acid solution or a mixture of sodium hypochlorite and Triton X compared to those treated with water. In addition, the removal of bacteria from the surfaces of treated eggs was highlighted by scanning electron microscopy. Conclusions The results clearly indicate how the first phases of the colony-establishment process are important in maintaining the symbiont load in laboratory populations and suggest that the use of products with antimicrobial activity should be avoided. The results also suggest that alternative rearing procedures for the olive fruit fly should be investigated.

ACS Style

Patrizia Sacchetti; Roberta Pastorelli; Gaia Bigiotti; Roberto Guidi; Sara Ruschioni; Carlo Viti; Antonio Belcari. Olive fruit fly rearing procedures affect the vertical transmission of the bacterial symbiont Candidatus Erwinia dacicola. BMC Biotechnology 2019, 19, 1 -13.

AMA Style

Patrizia Sacchetti, Roberta Pastorelli, Gaia Bigiotti, Roberto Guidi, Sara Ruschioni, Carlo Viti, Antonio Belcari. Olive fruit fly rearing procedures affect the vertical transmission of the bacterial symbiont Candidatus Erwinia dacicola. BMC Biotechnology. 2019; 19 (2):1-13.

Chicago/Turabian Style

Patrizia Sacchetti; Roberta Pastorelli; Gaia Bigiotti; Roberto Guidi; Sara Ruschioni; Carlo Viti; Antonio Belcari. 2019. "Olive fruit fly rearing procedures affect the vertical transmission of the bacterial symbiont Candidatus Erwinia dacicola." BMC Biotechnology 19, no. 2: 1-13.

Journal article
Published: 18 December 2019 in BMC Biotechnology
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Background The olive fly, Bactrocera oleae, is the most important insect pest in olive production, causing economic damage to olive crops worldwide. In addition to extensive research on B. oleae control methods, scientists have devoted much effort in the last century to understanding olive fly endosymbiosis with a bacterium eventually identified as Candidatus Erwinia dacicola. This bacterium plays a relevant role in olive fly fitness. It is vertically transmitted, and it benefits both larvae and adults in wild populations; however, the endosymbiont is not present in lab colonies, probably due to the antibiotics and preservatives required for the preparation of artificial diets. Endosymbiont transfer from wild B. oleae populations to laboratory-reared ones allows olive fly mass-rearing, thus producing more competitive flies for future Sterile Insect Technique (SIT) applications. Results We tested the hypothesis that Ca. E. dacicola might be transmitted from wild, naturally symbiotic adults to laboratory-reared flies. Several trials have been performed with different contamination sources of Ca. E. dacicola, such as ripe olives and gelled water contaminated by wild flies, wax domes containing eggs laid by wild females, cages dirtied by faeces dropped by wild flies and matings between lab and wild adults. PCR-DGGE, performed with the primer set 63F-GC/518R, demonstrated that the transfer of the endosymbiont from wild flies to lab-reared ones occurred only in the case of cohabitation. Conclusions Cohabitation of symbiotic wild flies and non-symbiotic lab flies allows the transfer of Ca. E. dacicola through adults. Moreover, PCR-DGGE performed with the primer set 63F-GC/518R was shown to be a consistent method for screening Ca. E. dacicola, also showing the potential to distinguish between the two haplotypes (htA and htB). This study represents the first successful attempt at horizontal transfer of Ca. E. dacicola and the first step in acquiring a better understanding of the endosymbiont physiology and its relationship with the olive fly. Our research also represents a starting point for the development of a laboratory symbiotic olive fly colony, improving perspectives for future applications of the Sterile Insect Technique.

ACS Style

Gaia Bigiotti; Roberta Pastorelli; Roberto Guidi; Antonio Belcari; Patrizia Sacchetti. Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont, Candidatus Erwinia dacicola. BMC Biotechnology 2019, 19, 1 -12.

AMA Style

Gaia Bigiotti, Roberta Pastorelli, Roberto Guidi, Antonio Belcari, Patrizia Sacchetti. Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont, Candidatus Erwinia dacicola. BMC Biotechnology. 2019; 19 (2):1-12.

Chicago/Turabian Style

Gaia Bigiotti; Roberta Pastorelli; Roberto Guidi; Antonio Belcari; Patrizia Sacchetti. 2019. "Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont, Candidatus Erwinia dacicola." BMC Biotechnology 19, no. 2: 1-12.

Journal article
Published: 27 August 2019 in Insects
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The dried fruit beetle (Carpophilus hemipterus) is considered a key pest of dates, infesting fruits both in the field and during storage. Control measures against the species rely on the use of chemicals or heat treatments based on sunlight, hot air or radio frequency (RF) applications. Previous investigations that have aimed to define control procedures for a total disinfestation using RF treatments with different exposure durations have shown the typical behavior of adults in some trials, which, under the influence of temperature increases, started to escape from infested fruits. We focused on the application of different RF voltage-time combinations to induce the emigration of C. hemipterus adults from dates in order to produce a complete disinfestation. The results showed that the application of 2500 V RF for 8–10 min to infested dates resulted in nearly 100% of adults escaping from fruits, thereby cleaning the commodity with low or no mortality inside and outside the dates. These achievements provide a new strategy for controlling key insect pests of dates that could be applied at an industrial scale, allowing for the quick disinfestation of fruits without affecting the fruit with harmful substances, such as residues from chemical treatments, and without unwanted side effects on date quality.

ACS Style

Marzia Cristiana Rosi; Francesco Garbati Pegna; Anita Nencioni; Roberto Guidi; Michele Bicego; Antonio Belcari; Patrizia Sacchetti. Emigration Effects Induced by Radio Frequency Treatment to Dates Infested by Carpophilus hemipterus. Insects 2019, 10, 273 .

AMA Style

Marzia Cristiana Rosi, Francesco Garbati Pegna, Anita Nencioni, Roberto Guidi, Michele Bicego, Antonio Belcari, Patrizia Sacchetti. Emigration Effects Induced by Radio Frequency Treatment to Dates Infested by Carpophilus hemipterus. Insects. 2019; 10 (9):273.

Chicago/Turabian Style

Marzia Cristiana Rosi; Francesco Garbati Pegna; Anita Nencioni; Roberto Guidi; Michele Bicego; Antonio Belcari; Patrizia Sacchetti. 2019. "Emigration Effects Induced by Radio Frequency Treatment to Dates Infested by Carpophilus hemipterus." Insects 10, no. 9: 273.

Original article
Published: 26 November 2018 in Medical and Veterinary Entomology
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Hippoboscidae flies parasitize various animal species. Knowledge about these insects remains sparse, although they are known to cause stress and damage to their hosts, and can also accidentally infest humans, causing different sanitary risks. Research conducted in Tuscany assessing the biology and distribution of Lipoptena cervi (Linnaeus, 1758) (Diptera: Hippoboscidae), the most common ectoparasite of ungulates in Italy, revealed the presence of Lipoptena fortisetosa Maa, 1965 in Italy for the first time. This study includes a morphological comparative description of L. cervi and L. fortisetosa, emphasizing the peculiar differences between the two species to facilitate their accurate identification. The most pertinent morphological differences between the two species are highlighted, such as the external features of the antennae, distribution of bristles, and different features in the external genitalia. In both species, scanning electron microscopy of mouthparts revealed strong adaptive convergence in the feeding apparatus. Modified palps and a very thin proboscis are described in relation to feeding behaviour.

ACS Style

A. Andreani; P. Sacchetti; A. Belcari. Comparative morphology of the deer ked Lipoptena fortisetosa first recorded from Italy. Medical and Veterinary Entomology 2018, 33, 140 -153.

AMA Style

A. Andreani, P. Sacchetti, A. Belcari. Comparative morphology of the deer ked Lipoptena fortisetosa first recorded from Italy. Medical and Veterinary Entomology. 2018; 33 (1):140-153.

Chicago/Turabian Style

A. Andreani; P. Sacchetti; A. Belcari. 2018. "Comparative morphology of the deer ked Lipoptena fortisetosa first recorded from Italy." Medical and Veterinary Entomology 33, no. 1: 140-153.

Other
Published: 11 July 2018
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Background: The symbiosis between the olive fruit fly,Bactrocera oleae, andCandidatusErwinia dacicola has been demonstrated as essential for the fly’s larval development and adult physiology. The mass rearing of the olive fruit fly has been hindered by several issues, including problems which could be related to the lack of the symbiont, presumably due to preservatives and antibiotics currently used in the laboratory. To better understand the mechanisms underlying symbiont removal or loss during the rearing of lab colonies of the olive fruit fly, we performed experiments that focused on bacterial transfer from wild female flies to their eggs. In this research, eggs laid by wild females were treated with propionic acid solution, which is often used as an antifungal agent, a mixture of sodium hypochlorite and Triton X, or water (as a control). The presence of the bacterial symbiont on eggs was evaluated by real-time PCR and scanning electron microscopy.Results: DGGE analysis showed a clear band with the same migration behavior present in all DGGE profiles but with a decreasing intensity. Molecular analyses performed by real-time PCR showed a significant reduction inCa. E. dacicola abundance in eggs treated with propionic acid solution or a mixture of sodium hypochlorite and Triton X compared to those treated with water. In addition, the removal of bacteria from the surfaces of treated eggs was highlighted by scanning electron microscopy.Conclusions: The results clearly indicate how the first phases of the colony-establishment process are important in maintaining the symbiont load in laboratory populations and suggest that the use of products with antimicrobial activity should be avoided. The results also suggest that alternative rearing procedures for the olive fruit fly should be investigated.

ACS Style

Patrizia Sacchetti; Roberta Pastorelli; Gaia Bigiotti; Roberto Guidi; Sara Ruschioni; Carlo Viti; Antonio Belcari. Olive fruit fly rearing procedures affect the vertical transmission of the bacterial symbiontCandidatusErwinia dacicola. 2018, 367417 .

AMA Style

Patrizia Sacchetti, Roberta Pastorelli, Gaia Bigiotti, Roberto Guidi, Sara Ruschioni, Carlo Viti, Antonio Belcari. Olive fruit fly rearing procedures affect the vertical transmission of the bacterial symbiontCandidatusErwinia dacicola. . 2018; ():367417.

Chicago/Turabian Style

Patrizia Sacchetti; Roberta Pastorelli; Gaia Bigiotti; Roberto Guidi; Sara Ruschioni; Carlo Viti; Antonio Belcari. 2018. "Olive fruit fly rearing procedures affect the vertical transmission of the bacterial symbiontCandidatusErwinia dacicola." , no. : 367417.

Other
Published: 18 May 2018
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BackgroundThe olive fly,Bactrocera oleae, is the most important insect pest in olive production, causing economic damage to olive crops worldwide. In addition to extensive research onB. oleaecontrol methods, scientists have devoted much effort in the last century to understanding olive fly endosymbiosis with a bacterium eventually identified asCandidatusErwinia dacicola. This bacterium plays a relevant role in olive fly fitness. It is vertically transmitted, and it benefits both larvae and adults in wild populations; however, the endosymbiont is not present in lab colonies, probably due to the antibiotics and preservatives required for the preparation of artificial diets. Endosymbiont transfer from wildB. oleaepopulations to laboratory-reared ones allows olive fly mass-rearing, thus producing more competitive flies for future Sterile Insect Technique (SIT) applications.ResultsWe tested the hypothesis thatCa.E. dacicola might be transmitted from wild, naturally symbiotic adults to laboratory-reared flies. Several trials have been performed with different contamination sources ofCa.E. dacicola, such as ripe olives and gelled water contaminated by wild flies, wax domes containing eggs laid by wild females, cages dirtied by faeces dropped by wild flies and matings between lab and wild adults. PCR-DGGE, performed with the primer set 63F-GC/518R, demonstrated that the transfer of the endosymbiont from wild flies to lab-reared ones occurred only in the case of cohabitation.ConclusionsCohabitation of symbiotic wild flies and non-symbiotic lab flies allows the transfer ofCa.E. dacicola through adults. Moreover, PCR-DGGE performed with the primer set 63F-GC/518R was shown to be a consistent method for screeningCa.E. dacicola, also showing the potential to distinguish between the two haplotypes (htA and htB). This study represents the first successful attempt at horizontal transfer ofCa.E. dacicola and the first step in acquiring a better understanding of the endosymbiont physiology and its relationship with the olive fly. Our research also represents a starting point for the development of a laboratory symbiotic olive fly colony, improving perspectives for future applications of the Sterile Insect Technique.

ACS Style

Gaia Bigiotti; Roberta Pastorelli; Roberto Guidi; Antonio Belcari; Patrizia Sacchetti. Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont,CandidatusErwinia dacicolax. 2018, 326090 .

AMA Style

Gaia Bigiotti, Roberta Pastorelli, Roberto Guidi, Antonio Belcari, Patrizia Sacchetti. Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont,CandidatusErwinia dacicolax. . 2018; ():326090.

Chicago/Turabian Style

Gaia Bigiotti; Roberta Pastorelli; Roberto Guidi; Antonio Belcari; Patrizia Sacchetti. 2018. "Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont,CandidatusErwinia dacicolax." , no. : 326090.

Journal article
Published: 01 March 2017 in Biosystems Engineering
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ACS Style

Francesco Garbati Pegna; Patrizia Sacchetti; Valentina Canuti; Serena Trapani; Carlo Bergesio; Antonio Belcari; Bruno Zanoni; Ferdinando Meggiolaro. Radio frequency irradiation treatment of dates in a single layer to control Carpophilus hemipterus. Biosystems Engineering 2017, 155, 1 -11.

AMA Style

Francesco Garbati Pegna, Patrizia Sacchetti, Valentina Canuti, Serena Trapani, Carlo Bergesio, Antonio Belcari, Bruno Zanoni, Ferdinando Meggiolaro. Radio frequency irradiation treatment of dates in a single layer to control Carpophilus hemipterus. Biosystems Engineering. 2017; 155 ():1-11.

Chicago/Turabian Style

Francesco Garbati Pegna; Patrizia Sacchetti; Valentina Canuti; Serena Trapani; Carlo Bergesio; Antonio Belcari; Bruno Zanoni; Ferdinando Meggiolaro. 2017. "Radio frequency irradiation treatment of dates in a single layer to control Carpophilus hemipterus." Biosystems Engineering 155, no. : 1-11.

Research article
Published: 28 August 2016 in Parkinson's Disease
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Parkinson’s disease (PD) is one of the most common neurodegenerative diseases characterized by the clinical triad: tremor, akinesia, and rigidity. Several studies have suggested that PD patients show disturbances in olfaction as one of the earliest, nonspecific nonmotor symptoms of disease onset. We sought to use the fruit flyDrosophila melanogasteras a model organism to explore olfactory function in LRRKloss-of-functionmutants, which was previously demonstrated to be a useful model for PD. Surprisingly, our results showed that the LRRK mutant, compared to the wild flies, presents a dramatic increase in the amplitude of the electroantennogram responses and this is coupled with a higher number of olfactory sensilla. In spite of the above reported results, the behavioural response to olfactory stimuli in mutant flies is impaired compared to that obtained in wild type flies. Thus, behaviour modifications and morphofunctional changes in the olfaction of LRRKloss-of-functionmutants might be used as an index to explore the progression of parkinsonism in this specific model, also with the aim of studying and developing new treatments.

ACS Style

Francescaelena De Rose; Valentina Corda; Paolo Solari; Patrizia Sacchetti; Antonio Belcari; Simone Poddighe; Sanjay Kasture; Paolo Solla; Francesco Marrosu; Anna Liscia. DrosophilaMutant Model of Parkinson’s Disease Revealed an Unexpected Olfactory Performance: Morphofunctional Evidences. Parkinson's Disease 2016, 2016, 1 -10.

AMA Style

Francescaelena De Rose, Valentina Corda, Paolo Solari, Patrizia Sacchetti, Antonio Belcari, Simone Poddighe, Sanjay Kasture, Paolo Solla, Francesco Marrosu, Anna Liscia. DrosophilaMutant Model of Parkinson’s Disease Revealed an Unexpected Olfactory Performance: Morphofunctional Evidences. Parkinson's Disease. 2016; 2016 ():1-10.

Chicago/Turabian Style

Francescaelena De Rose; Valentina Corda; Paolo Solari; Patrizia Sacchetti; Antonio Belcari; Simone Poddighe; Sanjay Kasture; Paolo Solla; Francesco Marrosu; Anna Liscia. 2016. "DrosophilaMutant Model of Parkinson’s Disease Revealed an Unexpected Olfactory Performance: Morphofunctional Evidences." Parkinson's Disease 2016, no. : 1-10.

Journal article
Published: 29 May 2015 in Arthropod-Plant Interactions
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The behavioural response of the sweet potato whitefly, Bemisia tabaci biotype B, to the volatiles emitted by three different species of Callistemon (C. laevis, C. pallidus and C. phoeniceus) was assayed in still-air, and Y-tube olfactometers. B. tabaci adults showed a preference for C. pallidus, while C. laevis and C. phoeniceus were less attractive to the insects when compared to a blank control or to C. pallidus. Volatile organic compounds emitted by the leaves of the three Callistemon species were analysed by solid phase microextraction coupled with GC–MS. 1,8-Cineole was detected in high percentages in C. phoeniceus and C. laevis headspaces, while linalool was abundant in the headspace of C. pallidus. Because of this difference, behavioural tests in the Y-tube olfactometer were carried out also with 1,8-cineole and linalool. Adults of B. tabaci were attracted by linalool, while they were repelled by 1,8-cineole compared with pure air or linalool. These results support the role of these volatiles in the host plant selection process by a herbivorous insect and lay the foundations for the development of sustainable control strategies based on the manipulation of insect pest behaviour.

ACS Style

Patrizia Sacchetti; Elisabetta Rossi; Lorenzo Bellini; Paolo Vernieri; Pier Luigi Cioni; Guido Flamini. Volatile organic compounds emitted by bottlebrush species affect the behaviour of the sweet potato whitefly. Arthropod-Plant Interactions 2015, 9, 393 -403.

AMA Style

Patrizia Sacchetti, Elisabetta Rossi, Lorenzo Bellini, Paolo Vernieri, Pier Luigi Cioni, Guido Flamini. Volatile organic compounds emitted by bottlebrush species affect the behaviour of the sweet potato whitefly. Arthropod-Plant Interactions. 2015; 9 (4):393-403.

Chicago/Turabian Style

Patrizia Sacchetti; Elisabetta Rossi; Lorenzo Bellini; Paolo Vernieri; Pier Luigi Cioni; Guido Flamini. 2015. "Volatile organic compounds emitted by bottlebrush species affect the behaviour of the sweet potato whitefly." Arthropod-Plant Interactions 9, no. 4: 393-403.

Journal article
Published: 08 November 2013 in Annals of Applied Biology
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One possible control strategy against the olive fly, Bactrocera oleae, the most serious olive crop pest, is the Sterile Insect Technique (SIT) application. However, a number of problems associated with this method remain that decrease the effectiveness of SIT, including the quality of reared insects. Taking the importance of the relationship between the olive fly and bacteria into consideration, the effects of probiotic diets enriched with Pseudomonas putida on B. oleae longevity and fecundity were evaluated. First, we found that the probiotic bacterium, P. putida, is conveyed from diets to the oesophageal bulb as well as to the fly midgut after feeding on the probiotic diet. Subsequently, B. oleae adults fed on either: (a) a standard full protein and sugar diet; (b) a sugar only diet; (c) a probiotic standard full protein and sugar diet; or (d) a probiotic sugar diet. Flies fed on probiotic diets were supplied with an inoculated gel containing P. putida; non‐inoculated gel was provided to the flies fed on non‐probiotic diets. B. oleae males and females that fed on sugar diets did not survive as long as those that fed on protein diets. A comparison of the longevity of adults fed on full diet and sugar with their respective probiotic diets revealed no significant difference. Males fed on the sugar only diet survived longer than males fed on probiotic sugar diet, and females fed on the full protein and sugar diet survived much longer than females fed on the full probiotic diet. As regarding fecundity, both full diets resulted in a higher number of eggs laid per female. Females fed on the probiotic sugar diet laid a higher number of eggs than females that fed on sugar only. The inoculated gel of the probiotic sugar diet contained a significantly higher quantity of leucine, isoleucine and proline than the non‐inoculated gel of the sugar only diet. The possible role of dietary bacteria in relation to functional aspects of olive fly physiology is discussed.

ACS Style

Paola Sacchetti; B. Ghiardi; A. Granchietti; F.M. Stefanini; Antonio Belcari. Development of probiotic diets for the olive fly: evaluation of their effects on fly longevity and fecundity. Annals of Applied Biology 2013, 164, 138 -150.

AMA Style

Paola Sacchetti, B. Ghiardi, A. Granchietti, F.M. Stefanini, Antonio Belcari. Development of probiotic diets for the olive fly: evaluation of their effects on fly longevity and fecundity. Annals of Applied Biology. 2013; 164 (1):138-150.

Chicago/Turabian Style

Paola Sacchetti; B. Ghiardi; A. Granchietti; F.M. Stefanini; Antonio Belcari. 2013. "Development of probiotic diets for the olive fly: evaluation of their effects on fly longevity and fecundity." Annals of Applied Biology 164, no. 1: 138-150.

Journal article
Published: 01 July 2013 in Journal of Insect Physiology
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The responses of olive fly (Bactrocera oleae) antennal and palpal olfactory receptors to odors emitted by\ud Pseudomonas putida bacterial filtrate and to volatiles from a host plant were evaluated using electrophysiological\ud and behavioral bioassays. Morphological identification of olfactory receptors was also performed.\ud The third antennal segment (flagellum) bears four types of multiporous sensilla: trichoid,\ud short basiconica, clavate and grooved. Maxillary palps have mechanosensory bristles and multiporous\ud basiconica sensilla. In wind-tunnel bioassays, olive fly responses to volatiles emitted by bacterial filtrate\ud were higher than those to culture medium. Bacterial filtrate was more attractive than ammonium carbonate\ud or a mixture of ethyl acetate and acetic acid in ethanol. GC–MS of bacterial filtrate identified some\ud of the chemicals produced by bacterial activity, including methyl thiolacetate, ammonia, 2-pentanone, 2-\ud heptanone, ethyl tiglate and methyl thiocyanate. Electrophysiological investigations proved that antennal\ud sensilla are responsive to bacterial filtrate odor, methyl thiolacetate, olive leaves and olives, as well as to\ud a-pinene, while acetic acid elicited an inhibitory response. Electropalpgrams recorded a specific response\ud to bacterial filtrate by mated males and females, as well as a dose-dependent response relationship to\ud methyl thiolacetate by mated females. The identification of new active volatile compounds in the semiochemical\ud system of the olive fly is promising for the development of innovative control strategies in areawide\ud management

ACS Style

Anna Liscia; Piera Angioni; Patrizia Sacchetti; Simone Poddighe; Aurelio Granchietti; Maria Dolores Setzu; Antonio Belcari. Characterization of olfactory sensilla of the olive fly: Behavioral and electrophysiological responses to volatile organic compounds from the host plant and bacterial filtrate. Journal of Insect Physiology 2013, 59, 705 -716.

AMA Style

Anna Liscia, Piera Angioni, Patrizia Sacchetti, Simone Poddighe, Aurelio Granchietti, Maria Dolores Setzu, Antonio Belcari. Characterization of olfactory sensilla of the olive fly: Behavioral and electrophysiological responses to volatile organic compounds from the host plant and bacterial filtrate. Journal of Insect Physiology. 2013; 59 (7):705-716.

Chicago/Turabian Style

Anna Liscia; Piera Angioni; Patrizia Sacchetti; Simone Poddighe; Aurelio Granchietti; Maria Dolores Setzu; Antonio Belcari. 2013. "Characterization of olfactory sensilla of the olive fly: Behavioral and electrophysiological responses to volatile organic compounds from the host plant and bacterial filtrate." Journal of Insect Physiology 59, no. 7: 705-716.

Journal article
Published: 30 April 2012 in Biological Control
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The pupal parasitoid Coptera occidentalis Muesebeck (Hymenoptera: Diapriidae) was reared in the 1970s in California for the biological control of the Walnut husk fly, Rhagoletis completa Cresson (Diptera: Tephritidae) and later introduced into Europe to be used as a biological control agent against fruit flies. Some behavioral aspects relating to the host location process were investigated in the laboratory in order to improve the chance of success in possible biological control programmes. The searching behavior of the female parasitoid was examined in an open arena in order to evaluate the role of larval trails or rotten-fruit trails (walnut) in the host location process. The response of C. occidentalis to four Tephritid hosts was evaluated: the natural host R. completa, the factitious host Ceratitis capitata (Wiedemann) and two fruit flies of economic importance, Rhagoletis cerasi Linnaeus and Bactrocera oleae (Rossi). In order to assess the response of the parasitoid, a number of behavioral parameters were considered, such as: arrestment behavior, resident time, walking distance, linear and angular speed. The larval trails of R. completa, as well as rotten-fruit liquid trails, were clearly detected by C. occidentalis females, even though the species has been reared for more than 80 generations on C. capitata. In addition, bioassays performed with C. capitata larvae showed a good behavioral response of parasitoid females to the larval trails. Finally, R. cerasi and B. oleae larval trails were barely detected in comparison to the traces left by R. completa and C. capitata larvae. The results are discussed in the light of the possible use of C. occidentalis in biological control programmes.

ACS Style

Aurelio Granchietti; Patrizia Sacchetti; Marzia Cristiana Rosi; Antonio Belcari. Fruit fly larval trail acts as a cue in the host location process of the pupal parasitoid Coptera occidentalis. Biological Control 2012, 61, 7 -14.

AMA Style

Aurelio Granchietti, Patrizia Sacchetti, Marzia Cristiana Rosi, Antonio Belcari. Fruit fly larval trail acts as a cue in the host location process of the pupal parasitoid Coptera occidentalis. Biological Control. 2012; 61 (1):7-14.

Chicago/Turabian Style

Aurelio Granchietti; Patrizia Sacchetti; Marzia Cristiana Rosi; Antonio Belcari. 2012. "Fruit fly larval trail acts as a cue in the host location process of the pupal parasitoid Coptera occidentalis." Biological Control 61, no. 1: 7-14.