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Large-scale efforts have been persistently undertaken for medical prophylaxis and treatment of COVID-19 disasters worldwide. A variety of novel viral spike protein-targeted vaccines have been extensively distributed for global inoculation based on accelerated approval. With concerns of emerging spike protein mutations, we revisited the early but inconclusive clinical interest in the repurposed combination of azithromycin (AZT) and zinc supplements with safety advantages. The aim of this study is to provide in vitro proof of concept for IκBα associated rapid and synergistic suppression of angiotensin-converting enzymes 2 (ACE2) following combination treatments with AZT plus zinc sulfate in two human airway cells with ACE2 expression, Calu-3 and H322M, representative cells of the human upper and lower airway origin respectively. Clinical timing of AZT combined with zinc is indicated based on suppression of the key cellular entry molecule, ACE2, of SARS-CoV-2.
Chia-Wei Chang; Ming-Cheng Lee; Bor-Ru Lin; Yen-Pei Lu; Yih-Jen Hsu; Chun-Yu Chuang; Tsung-Tao Huang; Yin-Kai Chen. The Effect of Azithromycin Plus Zinc Sulfate on ACE2 Expression through IκBα of Human Respiratory Cells in SARS-CoV-2: In Vitro Study. COVID 2021, 1, 263 -275.
AMA StyleChia-Wei Chang, Ming-Cheng Lee, Bor-Ru Lin, Yen-Pei Lu, Yih-Jen Hsu, Chun-Yu Chuang, Tsung-Tao Huang, Yin-Kai Chen. The Effect of Azithromycin Plus Zinc Sulfate on ACE2 Expression through IκBα of Human Respiratory Cells in SARS-CoV-2: In Vitro Study. COVID. 2021; 1 (1):263-275.
Chicago/Turabian StyleChia-Wei Chang; Ming-Cheng Lee; Bor-Ru Lin; Yen-Pei Lu; Yih-Jen Hsu; Chun-Yu Chuang; Tsung-Tao Huang; Yin-Kai Chen. 2021. "The Effect of Azithromycin Plus Zinc Sulfate on ACE2 Expression through IκBα of Human Respiratory Cells in SARS-CoV-2: In Vitro Study." COVID 1, no. 1: 263-275.
Dithiocarbamates (DTCs) are a group of fungicides with various toxicities, and are widely used in agriculture. Currently, most of the official DTC detection methods cannot identify residues of individual DTCs, such as dimethyldithiocarbamates (DMDCs), ethylene-bis-dithiocarbamates (EBDCs) and propylene-bis-dithiocarbamates (PBDCs), on crops. Surface-enhanced Raman scattering (SERS) provides fingerprint recognition from the scattering spectra of molecular vibrations, and it has excellent potential as a primary qualitative screening tool. In this study, glancing angle deposition (GLAD) was used to fabricate SERS substrates with [email protected] alloyed nanorods to provide cost-effective and rapid detection, and an optimal procedure for extracting DTC residues on the surface of crops was proposed. This study verified that under ordinary Raman spectroscopy conditions (excitation light, 785 nm; power, 20 mW; and integration, 3 min), extraction with tepid water and the addition of 0.1% sodium chloride can enable the detection of thiram (a DMDC), propineb (a PBDC) and mancozeb (an EBDC) on crops at trace concentrations of 0.05, 0.1 and 0.2 ppm, respectively, even on crops containing high levels of endogenous sulfides, such as cauliflower and white radish. Using the developed SERS-active substrate, qualitative and semi-quantitative results can be obtained by employing the specific characteristic peaks of the Raman scattering patterns to distinguish three types of DTCs. The method proposed in this study offers the advantages of simple operation, few consumables, high safety, and no interference from the matrix. Hence, this study provides an auxiliary approach for DTC detection.
Chao-Ming Tsen; Ching-Wei Yu; Sz-Ying Chen; Cheng-Li Lin; Chun-Yu Chuang. Application of surface-enhanced Raman scattering in rapid detection of dithiocarbamate pesticide residues in foods. Applied Surface Science 2021, 558, 149740 .
AMA StyleChao-Ming Tsen, Ching-Wei Yu, Sz-Ying Chen, Cheng-Li Lin, Chun-Yu Chuang. Application of surface-enhanced Raman scattering in rapid detection of dithiocarbamate pesticide residues in foods. Applied Surface Science. 2021; 558 ():149740.
Chicago/Turabian StyleChao-Ming Tsen; Ching-Wei Yu; Sz-Ying Chen; Cheng-Li Lin; Chun-Yu Chuang. 2021. "Application of surface-enhanced Raman scattering in rapid detection of dithiocarbamate pesticide residues in foods." Applied Surface Science 558, no. : 149740.
Bisphenol A (BPA) is a plasticizer used in the manufacture of polycarbonate and epoxy resins. It was found that higher urinary BPA levels are more likely to be associated with coronary artery disease (CVD). In recent years, the increasing incidence of CVD among young people is observed, which may be related with inflammation rather than the traditional triple-H risk factors. BPA is an endocrine-disrupting chemical, and can induce oxidative stress and chronic inflammation since its estrogenic effect. Inflammatory responses could come from the stimulation of IκB kinases (IKKs) by estrogen receptors (ERs). Therefore, this study investigated the association of BPA exposure with the gene expression of pro-inflammatory response (ERs and IKKs), an inflammation biomarker of CVD (C-reactive protein, CRP), and physiologic index potency of CVD development symptoms in young adults. This study divided BPA exposure levels into high and low groups based on the median plasma BPA level (4.34 ng/mL), and found that the high BPA group obviously had higher BMI, blood pressure, plasma CRP levels, and gene expression of ERβ and IKKβ. BMI and gene expression of IKKβ were also positively correlated with plasma CRP secretion. Furthermore, the study subjects with potential CVD development symptoms had the increased levels of BPA (OR 2.10, 95% CI 0.83–5.39), CRP (OR 2.61, 95% CI 1.03–10.6) and IKKβ (OR 4.29, 95% CI 1.51–15.6). These results indicated that exposure to BPA is potentially associated with expression of pro-inflammatory genes related to CRP secretion, which may promote the risk of CVD development symptoms in young adults. This study highlighted the possible connection between BPA exposure and CVD development but the mechanism between them needs to be further explored.
Chao-Ming Tsen; Jia-Hong Liu; Da-Peng Yang; How-Ran Chao; Jyh-Larng Chen; Wei-Chun Chou; Yi-Chen Ho; Chun-Yu Chuang. Study on the correlation of bisphenol A exposure, pro-inflammatory gene expression, and C-reactive protein with potential cardiovascular disease symptoms in young adults. Environmental Science and Pollution Research 2021, 28, 32580 -32591.
AMA StyleChao-Ming Tsen, Jia-Hong Liu, Da-Peng Yang, How-Ran Chao, Jyh-Larng Chen, Wei-Chun Chou, Yi-Chen Ho, Chun-Yu Chuang. Study on the correlation of bisphenol A exposure, pro-inflammatory gene expression, and C-reactive protein with potential cardiovascular disease symptoms in young adults. Environmental Science and Pollution Research. 2021; 28 (25):32580-32591.
Chicago/Turabian StyleChao-Ming Tsen; Jia-Hong Liu; Da-Peng Yang; How-Ran Chao; Jyh-Larng Chen; Wei-Chun Chou; Yi-Chen Ho; Chun-Yu Chuang. 2021. "Study on the correlation of bisphenol A exposure, pro-inflammatory gene expression, and C-reactive protein with potential cardiovascular disease symptoms in young adults." Environmental Science and Pollution Research 28, no. 25: 32580-32591.
Soluble amyloid-β oligomers (oAβ42)-induced neuronal death and inflammation response has been recognized as one of the major causes of Alzheimer’s disease (AD). In this work, a novel strategy adopting silica-coated iron oxide stir bar (MSB)-based AD therapy system via magnetic stirring-induced capture of oAβ42 into magnetic plaques (mpAβ42) and activation of microglia on cellular plaque clearance was developed. With oAβ42 being effectively converted into mpAβ42, the neurotoxicity toward neuronal cells was thus greatly reduced. In addition to the good preservation of neurite outgrowth through the diminished uptake of oAβ42, neurons treated with oAβ42 under magnetic stirring also exhibited comparable neuron-specific protein expression to those in the absence of oAβ42. The phagocytic uptake of mpAβ42 by microglia was enhanced significantly as compared to the counterpart of oAβ42, and the M1 polarization of microglia often occurring after the uptake of oAβ42 restricted to an appreciable extent. As a result, the inflammation induced by pro-inflammatory cytokines was greatly alleviated.
Yuan-Chung Tsai; Jing-Chian Luo; Te-I Liu; I-Lin Lu; Ming-Yin Shen; Chun-Yu Chuang; Chorng-Shyan Chern; Hsin-Cheng Chiu. Capturing Amyloid-β Oligomers by Stirring with Microscaled Iron Oxide Stir Bars into Magnetic Plaques to Reduce Cytotoxicity toward Neuronal Cells. Nanomaterials 2020, 10, 1284 .
AMA StyleYuan-Chung Tsai, Jing-Chian Luo, Te-I Liu, I-Lin Lu, Ming-Yin Shen, Chun-Yu Chuang, Chorng-Shyan Chern, Hsin-Cheng Chiu. Capturing Amyloid-β Oligomers by Stirring with Microscaled Iron Oxide Stir Bars into Magnetic Plaques to Reduce Cytotoxicity toward Neuronal Cells. Nanomaterials. 2020; 10 (7):1284.
Chicago/Turabian StyleYuan-Chung Tsai; Jing-Chian Luo; Te-I Liu; I-Lin Lu; Ming-Yin Shen; Chun-Yu Chuang; Chorng-Shyan Chern; Hsin-Cheng Chiu. 2020. "Capturing Amyloid-β Oligomers by Stirring with Microscaled Iron Oxide Stir Bars into Magnetic Plaques to Reduce Cytotoxicity toward Neuronal Cells." Nanomaterials 10, no. 7: 1284.
Paraquat (PQ), has been used in many countries for controlling weed growth in agriculture because of its quick-acting and nonselective contact with green plant tissue.
Chao-Ming Tsen; Ching-Wei Yu; Wei-Chen Chuang; Ming-Jun Chen; Shao-Kai Lin; Tsyr-Horng Shyu; Yung-Hsiang Wang; Cheng-Chien Li; Wei-Chung Chao; Chun-Yu Chuang. A simple approach for the ultrasensitive detection of paraquat residue in adzuki beans by surface-enhanced Raman scattering. The Analyst 2018, 144, 426 -438.
AMA StyleChao-Ming Tsen, Ching-Wei Yu, Wei-Chen Chuang, Ming-Jun Chen, Shao-Kai Lin, Tsyr-Horng Shyu, Yung-Hsiang Wang, Cheng-Chien Li, Wei-Chung Chao, Chun-Yu Chuang. A simple approach for the ultrasensitive detection of paraquat residue in adzuki beans by surface-enhanced Raman scattering. The Analyst. 2018; 144 (2):426-438.
Chicago/Turabian StyleChao-Ming Tsen; Ching-Wei Yu; Wei-Chen Chuang; Ming-Jun Chen; Shao-Kai Lin; Tsyr-Horng Shyu; Yung-Hsiang Wang; Cheng-Chien Li; Wei-Chung Chao; Chun-Yu Chuang. 2018. "A simple approach for the ultrasensitive detection of paraquat residue in adzuki beans by surface-enhanced Raman scattering." The Analyst 144, no. 2: 426-438.
This study systemically investigated the ambient PM (n=108) with comprehensive analyses of the chemical composition, identification of the potential contributors, and estimation of the resultant respiratory physician visits in the residential regions near energy-consuming and high-polluting industries in central Taiwan. The positive matrix fraction (PMF) model with chemical profiles of trace metals, water-soluble ions, and organic/elemental carbons (OC/EC) was applied to quantify the potential sources of PM. The influences of local sources were also explored using the conditional probability function (CPF). Associations between the daily PM concentration and the risk of respiratory physician visits for the elderly (≥65years of age) were estimated using time-series analysis. A seasonal variation, with higher concentrations of PM, metals (As, Cd, Sb, and Pb), OC/EC and ions (i.e., NO, SO and NH) in the winter than in the spring and summer, was observed. Overall, an increase of 10μgm in the same-day PM was associated with an ~2% (95% CI: 1.5%-2.5%) increase in respiratory physician visits. Considering the health benefits of an effective reduction, we suggest that the emission from coal combustion (23.5%), iron ore and steel industry (17.1%), and non-ferrous metallurgy (14.4%), accounting for ~70% of the primary PM in the winter are prioritized to control.
Chin-Yu Hsu; Hung-Che Chiang; Mu-Jean Chen; Chun-Yu Chuang; Chao-Ming Tsen; Guor-Cheng Fang; Ying I. Tsai; Nai-Tzu Chen; Tzu-Yu Lin; Sheng-Lun Lin; Yu-Cheng Chen. Ambient PM2.5 in the residential area near industrial complexes: Spatiotemporal variation, source apportionment, and health impact. Science of The Total Environment 2017, 590-591, 204 -214.
AMA StyleChin-Yu Hsu, Hung-Che Chiang, Mu-Jean Chen, Chun-Yu Chuang, Chao-Ming Tsen, Guor-Cheng Fang, Ying I. Tsai, Nai-Tzu Chen, Tzu-Yu Lin, Sheng-Lun Lin, Yu-Cheng Chen. Ambient PM2.5 in the residential area near industrial complexes: Spatiotemporal variation, source apportionment, and health impact. Science of The Total Environment. 2017; 590-591 ():204-214.
Chicago/Turabian StyleChin-Yu Hsu; Hung-Che Chiang; Mu-Jean Chen; Chun-Yu Chuang; Chao-Ming Tsen; Guor-Cheng Fang; Ying I. Tsai; Nai-Tzu Chen; Tzu-Yu Lin; Sheng-Lun Lin; Yu-Cheng Chen. 2017. "Ambient PM2.5 in the residential area near industrial complexes: Spatiotemporal variation, source apportionment, and health impact." Science of The Total Environment 590-591, no. : 204-214.
Bisphenol A (BPA) are commonly used in the manufacture of polycarbonate plastics. Higher BPA exposure levels have been found in patients with endometrial hyperplasia that is one of risk factors of endometrial cancer (EC). Aberrant microRNAs (miRNAs) regulation has been observed in the development of cancer. Thus, this study investigated whether BPA exposure can disrupt miRNA regulation and its gene expression regarding to EC carcinogenic progress. Microarray experiments of miRNA and mRNA were performed in human endometrial cancer RL95-2 cells with treatment of low-to-moderate (10, 10 and 10nM) BPA to explore the aberrant genes corresponding to human EC progression. According to the analysis of KEGG pathway and Cytoscape gene network, this study identified that BPA exposure reduced miR-149 expression to down-regulate DNA repair gene ARF6 (ADP-ribosylation factor 6) and tumor protein p53 (TP53), and up-regulate CCNE2 (cyclin E2) potentially to interrupt cell cycle. BPA also increased miR-107 to suppress hedgehog signaling factors, suppressor of fused homolog (SUFU) and GLI family zinc finger 3 (GLI3) to activate hedgehog signaling for cell proliferation underlying carcinogenesis. Furthermore, the BPA-induced cell proliferation was attenuated by transfection with miR-149 mimic and miR-107 inhibitor. These findings provided an insight into potential epigenetic mechanism of BPA exposure on the risk of endometrial carcinogenesis.
Wei-Chun Chou; Pei-Hsuan Lee; Yan-Yan Tan; Ho-Chen Lin; Chung-Wei Yang; Kuan-Hsueh Chen; Chun-Yu Chuang. An integrative transcriptomic analysis reveals bisphenol A exposure-induced dysregulation of microRNA expression in human endometrial cells. Toxicology in Vitro 2017, 41, 133 -142.
AMA StyleWei-Chun Chou, Pei-Hsuan Lee, Yan-Yan Tan, Ho-Chen Lin, Chung-Wei Yang, Kuan-Hsueh Chen, Chun-Yu Chuang. An integrative transcriptomic analysis reveals bisphenol A exposure-induced dysregulation of microRNA expression in human endometrial cells. Toxicology in Vitro. 2017; 41 ():133-142.
Chicago/Turabian StyleWei-Chun Chou; Pei-Hsuan Lee; Yan-Yan Tan; Ho-Chen Lin; Chung-Wei Yang; Kuan-Hsueh Chen; Chun-Yu Chuang. 2017. "An integrative transcriptomic analysis reveals bisphenol A exposure-induced dysregulation of microRNA expression in human endometrial cells." Toxicology in Vitro 41, no. : 133-142.
An in vitro blood-brain barrier (BBB) model being composed of co-culture with endothelial (bEnd.3) and astrocyte-like (ALT) cells was established to evaluate the toxicity and permeability of Ag nanoparticles (AgNPs; 8nm) and TiO2 nanoparticles (TiO2NPs; 6nm and 35nm) in normal and inflammatory central nervous system. Lipopolysaccharide (LPS) was pre-treated to simulate the inflammatory responses. Both AgNPs and Ag ions can decrease transendothelial electrical resistance (TEER) value, and cause discontinuous tight junction proteins (claudin-5 and zonula occludens-1) of BBB. However, only the Ag ions induced inflammatory cytokines to release, and had less cell-to-cell permeability than AgNPs, which indicated that the toxicity of AgNPs was distinct from Ag ions. LPS itself disrupted BBB, while co-treatment with AgNPs and LPS dramatically enhanced the disruption and permeability coefficient. On the other hand, TiO2NPs exposure increased BBB penetration by size, and disrupted tight junction proteins without size dependence, and many of TiO2NPs accumulated in the endothelial cells were observed. This study provided the new insight of toxic potency of AgNPs and TiO2NPs in BBB.
I-Chieh Chen; I-Lun Hsiao; Ho-Chen Lin; Chien-Hou Wu; Chun-Yu Chuang; Yuh-Jeen Huang. Influence of silver and titanium dioxide nanoparticles on in vitro blood-brain barrier permeability. Environmental Toxicology and Pharmacology 2016, 47, 108 -118.
AMA StyleI-Chieh Chen, I-Lun Hsiao, Ho-Chen Lin, Chien-Hou Wu, Chun-Yu Chuang, Yuh-Jeen Huang. Influence of silver and titanium dioxide nanoparticles on in vitro blood-brain barrier permeability. Environmental Toxicology and Pharmacology. 2016; 47 ():108-118.
Chicago/Turabian StyleI-Chieh Chen; I-Lun Hsiao; Ho-Chen Lin; Chien-Hou Wu; Chun-Yu Chuang; Yuh-Jeen Huang. 2016. "Influence of silver and titanium dioxide nanoparticles on in vitro blood-brain barrier permeability." Environmental Toxicology and Pharmacology 47, no. : 108-118.
Silver nanoparticles (AgNPs) are commonly used in daily living products. AgNPs can induce inflammatory response in neuronal cells, and potentially develop neurological disorders. The gene networks in response to AgNP-induced neurodegenerative progression have not been clarified in various brain neural cells. This study found that 3–5 nm AgNPs were detectable to enter the nuclei of mouse neuronal cells after 24 h of exposure. The differentially expressed genes in mouse brain neural cell exposure to AgNPs were further identified using Phalanx Mouse OneArray® chip, and permitted to explore the gene network pathway regulating in neurodegenerative progression according to Cytoscape analysis. In focal adhesion pathway of ALT astrocytes, AgNPs induced the gene expression of RasGRF1 and reduced its downstream BCL2 gene for apoptosis. In cytosolic DNA sensing pathway of microglial BV2 cells, AgNPs reduced the gene expression of TREX1 and decreased IRF7 to release pro-inflammatory cytokines for inflammation and cellular activation. In MAPK pathway of neuronal N2a cells, AgNPs elevated GADD45α gene expression, and attenuated its downstream PTPRR gene to interfere with neuron growth and differentiation. Moreover, AgNPs induced beta amyloid deposition in N2a cells, and decreased PSEN1 and PSEN2, which may disrupt calcium homeostasis and presynaptic dysfunction for Alzheimer's disease development. These findings suggested that AgNP exposure reveals the potency to induce the progression of neurodegenerative disorder.
Ho-Chen Lin; Chin-Lin Huang; Yuh-Jeen Huang; I-Lun Hsiao; Chung-Wei Yang. Transcriptomic gene-network analysis of exposure to silver nanoparticle reveals potentially neurodegenerative progression in mouse brain neural cells. Toxicology in Vitro 2016, 34, 289 -299.
AMA StyleHo-Chen Lin, Chin-Lin Huang, Yuh-Jeen Huang, I-Lun Hsiao, Chung-Wei Yang. Transcriptomic gene-network analysis of exposure to silver nanoparticle reveals potentially neurodegenerative progression in mouse brain neural cells. Toxicology in Vitro. 2016; 34 ():289-299.
Chicago/Turabian StyleHo-Chen Lin; Chin-Lin Huang; Yuh-Jeen Huang; I-Lun Hsiao; Chung-Wei Yang. 2016. "Transcriptomic gene-network analysis of exposure to silver nanoparticle reveals potentially neurodegenerative progression in mouse brain neural cells." Toxicology in Vitro 34, no. : 289-299.
Spinal muscular atrophy (SMA) is an inherited neuromuscular disease resulting from a recessive mutation in the SMN1 gene. This disease affects multiple organ systems with varying degrees of severity. Exploration of the molecular pathological changes occurring in different cell types in SMA is crucial for developing new therapies. This study collected 39 human microarray datasets from ArrayExpress and GEO databases to build an integrative transcriptomic analysis for recognizing novel SMA targets. The transcriptomic analysis was conducted through combining weighted correlation network analysis (WGCNA) for gene module detection, gene set enrichment analysis (GSEA) for functional categorization and filtration, and Cytoscape (visual interaction gene network analysis) for target gene identification. Seven novel target genes (Bmp4, Serpine1, Gata6, Ptgs2, Bcl2, IL6 and Cntn1) of SMA were revealed, and are all known in the regulation of TNFα for controlling neural, cardiac and bone development. Sequentially, the differentially expressed patterns of these 7 target genes in mouse tissues (e.g., spinal cord, heart, muscles and bone) were validated in SMA mice of different severities (pre-symptomatic, mildly symptomatic, and severely symptomatic). In severely symptomatic SMA mice, TNFα was up-regulated with attenuation of Bmp4 and increase of Serpine1 and Gata6 (a pathway in neural and cardiac development), but not in pre-symptomatic and mildly symptomatic SMA mice. The severely symptomatic SMA mice also had the elevated levels of Ptgs2 and Bcl2 (a pathway in skeletal development) as well as IL6 and Cntn1 (a pathway in nervous system development). Thus, the 7 genes identified in this study might serve as potential target genes for future investigations of disease pathogenesis and SMA therapy.
Chung-Wei Yang; Chien-Lin Chen; Wei-Chun Chou; Ho-Chen Lin; Yuh-Jyh Jong; Li-Kai Tsai; Chun-Yu Chuang. An Integrative Transcriptomic Analysis for Identifying Novel Target Genes Corresponding to Severity Spectrum in Spinal Muscular Atrophy. PLOS ONE 2016, 11, e0157426 .
AMA StyleChung-Wei Yang, Chien-Lin Chen, Wei-Chun Chou, Ho-Chen Lin, Yuh-Jyh Jong, Li-Kai Tsai, Chun-Yu Chuang. An Integrative Transcriptomic Analysis for Identifying Novel Target Genes Corresponding to Severity Spectrum in Spinal Muscular Atrophy. PLOS ONE. 2016; 11 (6):e0157426.
Chicago/Turabian StyleChung-Wei Yang; Chien-Lin Chen; Wei-Chun Chou; Ho-Chen Lin; Yuh-Jyh Jong; Li-Kai Tsai; Chun-Yu Chuang. 2016. "An Integrative Transcriptomic Analysis for Identifying Novel Target Genes Corresponding to Severity Spectrum in Spinal Muscular Atrophy." PLOS ONE 11, no. 6: e0157426.
Although, titanium dioxide nanoparticles (TiO2NPs) are nanomaterials commonly used in consumer products, little is known about their hazardous effects, especially on central nervous systems. To examine this issue, ALT astrocyte-like, BV-2 microglia and differentiated N2a neuroblastoma cells were exposed to 6 nm of 100% anatase TiO2NPs. A lipopolysaccharide (LPS) was pre-treated to activate glial cells before NP treatment for mimicking NP exposure under brain injury. We found that ALT and BV-2 cells took up more NPs than N2a cells and caused lower cell viability. TiO2NPs induced IL-1β in the three cell lines and IL-6 in N2a. LPS-activated BV-2 took up more TiO2NPs than normal BV-2 and released more intra/extracellular reactive oxygen species (ROS), IL-1β, IL-6 and MCP-1 than did activated BV-2. Involvement of clathrin- and caveolae-dependent endocytosis in ALT and clathrin-dependent endocytosis and phagocytosis in BV-2 both had a slow NP translocation rate to lysosome, which may cause slow ROS production (after 24 h). Although TiO2NPs did not directly cause N2a viability loss, by indirect NP exposure to the bottom chamber of LPS-activated BV-2 in the Transwell system, they caused late apoptosis and loss of cell viability in the upper N2a chamber due to H2O2 and/or TNF-α release from BV-2. However, none of the adverse effects in N2a or BV-2 cells was observed when TiO2NPs were exposed to ALT-N2a or ALT-BV-2 co-culture. These results demonstrate that neuron damage can result from TiO2NP-mediated ROS and/or cytokines release from microglia, but not from astrocytes.
I-Lun Hsiao; Chia-Cheng Chang; Chung-Yi Wu; Yi-Kong Hsieh; Chun-Yu Chuang; Chu-Fang Wang; Yuh-Jeen Huang. Indirect effects of TiO2 nanoparticle on neuron-glial cell interactions. Chemico-Biological Interactions 2016, 254, 34 -44.
AMA StyleI-Lun Hsiao, Chia-Cheng Chang, Chung-Yi Wu, Yi-Kong Hsieh, Chun-Yu Chuang, Chu-Fang Wang, Yuh-Jeen Huang. Indirect effects of TiO2 nanoparticle on neuron-glial cell interactions. Chemico-Biological Interactions. 2016; 254 ():34-44.
Chicago/Turabian StyleI-Lun Hsiao; Chia-Cheng Chang; Chung-Yi Wu; Yi-Kong Hsieh; Chun-Yu Chuang; Chu-Fang Wang; Yuh-Jeen Huang. 2016. "Indirect effects of TiO2 nanoparticle on neuron-glial cell interactions." Chemico-Biological Interactions 254, no. : 34-44.
How-Ran Chao; Danielle E. Que; Yan-You Gou; Chun-Yu Chuang; To-Yao Chang; Yi-Chyun Hsu. Indoor and Outdoor Concentrations of Polybrominated Diphenyl Ethers on Respirable Particulate in Central and Southern Taiwan. Aerosol and Air Quality Research 2016, 16, 3187 -3197.
AMA StyleHow-Ran Chao, Danielle E. Que, Yan-You Gou, Chun-Yu Chuang, To-Yao Chang, Yi-Chyun Hsu. Indoor and Outdoor Concentrations of Polybrominated Diphenyl Ethers on Respirable Particulate in Central and Southern Taiwan. Aerosol and Air Quality Research. 2016; 16 (12):3187-3197.
Chicago/Turabian StyleHow-Ran Chao; Danielle E. Que; Yan-You Gou; Chun-Yu Chuang; To-Yao Chang; Yi-Chyun Hsu. 2016. "Indoor and Outdoor Concentrations of Polybrominated Diphenyl Ethers on Respirable Particulate in Central and Southern Taiwan." Aerosol and Air Quality Research 16, no. 12: 3187-3197.
Silver nanoparticles (AgNPs) have antibacterial characteristics, and currently are applied in Ag-containing products. This study found neural cells can uptake 3-5 nm AgNPs, and investigated the potential effects of AgNPs on gene expression of inflammation and neurodegenerative disorder in murine brain ALT astrocytes, microglial BV-2 cells and neuron N2a cells. After AgNPs (5, 10, 12.5 μg/ml) exposure, these neural cells had obviously increased IL-1β secretion, and induced gene expression of C-X-C motif chemokine 13 (CXCL13), macrophage receptor with collagenous structure (MARCO) and glutathione synthetase (GSS) for inflammatory response and oxidative stress neutralization. Additionally, this study found amyloid-β (Aβ) plaques for pathological feature of Alzheimer's disease (AD) deposited in neural cells after AgNPs treatment. After AgNPs exposure, the gene expression of amyloid precursor protein (APP) was induced, and otherwise, neprilysin (NEP) and low-density lipoprotein receptor (LDLR) were reduced in neural cells as well as protein level. These results suggested AgNPs could alter gene and protein expressions of Aβ deposition potentially to induce AD progress in neural cells. It's necessary to take notice of AgNPs distribution in the environment.
Chin-Lin Huang; I-Lun Hsiao; Ho-Chen Lin; Chu-Fang Wang; Yuh-Jeen Huang. Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells. Environmental Research 2015, 136, 253 -263.
AMA StyleChin-Lin Huang, I-Lun Hsiao, Ho-Chen Lin, Chu-Fang Wang, Yuh-Jeen Huang. Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells. Environmental Research. 2015; 136 ():253-263.
Chicago/Turabian StyleChin-Lin Huang; I-Lun Hsiao; Ho-Chen Lin; Chu-Fang Wang; Yuh-Jeen Huang. 2015. "Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells." Environmental Research 136, no. : 253-263.
Bisphenol A (BPA) is a ubiquitous endocrine disrupting chemical in our daily life, and its health effect in response to prenatal exposure is still controversial. Early-life BPA exposure may impact brain development and contribute to childhood neurological disorders. The aim of the present study was to investigate molecular target genes of neuronal development in trans-placental exposure to BPA. A meta-analysis of three public microarray datasets was performed to screen for differentially expressed genes (DEGs) in exposure to BPA. The candidate genes of neuronal development were identified from gene ontology analysis in a reconstructed neuronal sub-network, and their gene expressions were determined using real-time PCR in 20 umbilical cord blood samples dichotomized into high and low BPA level groups upon the median 16.8 nM. Among 36 neuronal transcripts sorted from DAVID ontology clusters of 457 DEGs using the analysis of Bioconductor limma package, we found two neuronal genes, sex determining region Y-box 2 (Sox2) and paired box 6 (Pax6), had preferentially down-regulated expression (Bonferroni correction p-value <10−4 and log2-transformed fold change ≤−1.2) in response to BPA exposure. Fetal cord blood samples had the obviously attenuated gene expression of Sox2 and Pax6 in high BPA group referred to low BPA group. Visualized gene network of Cytoscape analysis showed that Sox2 and Pax6 which were contributed to neural precursor cell proliferation and neuronal differentiation might be down-regulated through sonic hedgehog (Shh), vascular endothelial growth factor A (VEGFA) and Notch signaling. These results indicated that trans-placental BPA exposure down-regulated gene expression of Sox2 and Pax6 potentially underlying the adverse effect on childhood neuronal development.
Chung-Wei Yang; Wei-Chun Chou; Kuan-Hsueh Chen; An-Lin Cheng; I-Fang Mao; How-Ran Chao; Chun-Yu Chuang. Visualized Gene Network Reveals the Novel Target Transcripts Sox2 and Pax6 of Neuronal Development in Trans-Placental Exposure to Bisphenol A. PLOS ONE 2014, 9, e100576 .
AMA StyleChung-Wei Yang, Wei-Chun Chou, Kuan-Hsueh Chen, An-Lin Cheng, I-Fang Mao, How-Ran Chao, Chun-Yu Chuang. Visualized Gene Network Reveals the Novel Target Transcripts Sox2 and Pax6 of Neuronal Development in Trans-Placental Exposure to Bisphenol A. PLOS ONE. 2014; 9 (7):e100576.
Chicago/Turabian StyleChung-Wei Yang; Wei-Chun Chou; Kuan-Hsueh Chen; An-Lin Cheng; I-Fang Mao; How-Ran Chao; Chun-Yu Chuang. 2014. "Visualized Gene Network Reveals the Novel Target Transcripts Sox2 and Pax6 of Neuronal Development in Trans-Placental Exposure to Bisphenol A." PLOS ONE 9, no. 7: e100576.
Endometrial cancers (ECs) are the most common form of gynecologic malignancy. Recent studies have reported that ECs reveal distinct markers for molecular pathogenesis, which in turn is linked to the various histological types of ECs. To understand further the molecular events contributing to ECs and endometrial tumorigenesis in general, a more precise identification of cancer-associated molecules and signaling networks would be useful for the detection and monitoring of malignancy, improving clinical cancer therapy, and personalization of treatments.
Wei-Chun Chou; An-Lin Cheng; Marco Brotto; Chun-Yu Chuang. Visual gene-network analysis reveals the cancer gene co-expression in human endometrial cancer. BMC Genomics 2014, 15, 300 -300.
AMA StyleWei-Chun Chou, An-Lin Cheng, Marco Brotto, Chun-Yu Chuang. Visual gene-network analysis reveals the cancer gene co-expression in human endometrial cancer. BMC Genomics. 2014; 15 (1):300-300.
Chicago/Turabian StyleWei-Chun Chou; An-Lin Cheng; Marco Brotto; Chun-Yu Chuang. 2014. "Visual gene-network analysis reveals the cancer gene co-expression in human endometrial cancer." BMC Genomics 15, no. 1: 300-300.
The composting procedure in food waste plants generates airborne bioaerosols that have the potential to damage human airway epithelial cells. Persistent inflammation and repair responses induce airway remodeling and damage to the respiratory system. This study elucidated the expression changes of airway remodeling genes in human lung mucoepidermoid NCI-H292 cells exposed to bioaerosols from a composting plant. Different types of microorganisms were detectable in the composting plant, using the agar culture method. Real-time polymerase chain reaction was used to quantify the level of Aspergillus fumigatus and the profile of remodeling genes. The real-time PCR results indicated that the amount of A. fumigatus in the composting hall was less than 102 conidia. The endotoxins in the field bioaerosols were determined using a limulus amebocyte lysate test. The endotoxin levels depended on the type of particulate matter (PM), with coarse particles (2.5–10 μm) having higher endotoxin levels than did fine particles (0.5–2.5 μm). After exposure to the conditioned medium of field bioaerosol samples, NCI-H292 cells showed increased pro-inflammatory interleukin (IL)-6 release and activated epidermal growth factor receptor (EGFR), transforming growth factor (TGF)-β1 and cyclin-dependent kinase inhibitor 1 (p21WAF1/CIP1) gene expression, but not of matrix metallopeptidase (MMP)-9. Airborne endotoxin levels were higher inside the composting hall than they were in other areas, and they were associated with PM. This suggested that airborne bioaerosols in the composting plant contained endotoxins and microorganisms besides A. fumigatus that cause the inflammatory cytokine secretion and augment the expression of remodeling genes in NCI-H292 cells. It is thus necessary to monitor potentially hazardous materials from bioaerosols in food composting plants, which could affect the health of workers.
Ming-Wei Chang; Chung-Ru Lee; Hsueh-Fen Hung; Kuo-Sheng Teng; Hsin Huang; Chun-Yu Chuang. Bioaerosols from a Food Waste Composting Plant Affect Human Airway Epithelial Cell Remodeling Genes. International Journal of Environmental Research and Public Health 2013, 11, 337 -354.
AMA StyleMing-Wei Chang, Chung-Ru Lee, Hsueh-Fen Hung, Kuo-Sheng Teng, Hsin Huang, Chun-Yu Chuang. Bioaerosols from a Food Waste Composting Plant Affect Human Airway Epithelial Cell Remodeling Genes. International Journal of Environmental Research and Public Health. 2013; 11 (1):337-354.
Chicago/Turabian StyleMing-Wei Chang; Chung-Ru Lee; Hsueh-Fen Hung; Kuo-Sheng Teng; Hsin Huang; Chun-Yu Chuang. 2013. "Bioaerosols from a Food Waste Composting Plant Affect Human Airway Epithelial Cell Remodeling Genes." International Journal of Environmental Research and Public Health 11, no. 1: 337-354.
Elemental determination of 80 third molars, collected from local dental clinics in Hsinchu City, Taiwan during 2009 to 2010, was conducted using inductively coupled plasma—mass spectrometry (ICP-MS). Results show that the mean concentrations of P, Ca, Sr, Ba and Pb in enamel are respectively 14.63% ± 2.19%, 27.91% ± 4.03%, 108.31 ± 35.71 ppm, 1.96 ± 1.01 ppm, and 0.72 ± 0.49 ppm. The concentrations of P, Ca and Sr are higher in enamel than in dentine, on the other hand, the concentrations of Ba and Pb are higher in dentine than in enamel. In enamel and dentine the concentrations of P, Ca and Ca/P ratio are kept constant. In enamelthe concentrations of Sr and Sr/Ca increase by age statistically but the concentrations of Ba and Ba/Ca are not. Pb concentrations in both enamel and dentine increase by age and also increase with significant differences among each birth era. This may indicate the dates of environmental exposure. The levels of Pb in this study are lower than the previous published findings before 1979. The concentrations and distribution of elements in enamel and dentine of third molars other than deciduous or permanent teeth can provide reliable base references to past and future studies.
Hwa-Yen Liu; Jiunn-Hsing Chao; Chun-Yu Chuang; Hung-Lin Chiu; Chung-Wei Yang; Yuh-Chang Sun. Study of P, Ca, Sr, Ba and Pb Levels in Enamel and Dentine of Human Third Molars for Environmental and Archaeological Research. Advances in Anthropology 2013, 03, 71 -77.
AMA StyleHwa-Yen Liu, Jiunn-Hsing Chao, Chun-Yu Chuang, Hung-Lin Chiu, Chung-Wei Yang, Yuh-Chang Sun. Study of P, Ca, Sr, Ba and Pb Levels in Enamel and Dentine of Human Third Molars for Environmental and Archaeological Research. Advances in Anthropology. 2013; 03 (02):71-77.
Chicago/Turabian StyleHwa-Yen Liu; Jiunn-Hsing Chao; Chun-Yu Chuang; Hung-Lin Chiu; Chung-Wei Yang; Yuh-Chang Sun. 2013. "Study of P, Ca, Sr, Ba and Pb Levels in Enamel and Dentine of Human Third Molars for Environmental and Archaeological Research." Advances in Anthropology 03, no. 02: 71-77.
Integrins are a family of transmembrane adhesion proteins that mediate cell adhesion and intracellular signaling. Integrin-αvβ3 is expressed on the surface of human glioblastoma cells, and can be further induced by chemical stress. The Arg-Gly-Asp (RGD) motif-containing peptides are specifically bound to integrin-αvβ3, and to inhibit neovasculature underlying competition to normal extracellular matrix proteins. This study employed two types of RGD peptides, cyclic RGD (c(RGDyK)) and bi-cyclic RGD (E[c(RGDyK)]2) peptide, to human glioblastoma U87MG cells with combination of low dose Paclitaxel (PTX) pre-treatment to augment therapeutic activity for RGD peptide-induced apoptosis. Human glioblastoma U87MG cells were treated with RGD peptides in the absence or presence of initial exposure to low-dose 10 nM PTX. Results showed that integrin-αvβ3 expressing on the surface of U87MG cells was induced by 10 nM PTX pre-treatment for 12 hrs. Additionally, the U87MG cells pre-treated with PTX and followed by RGD peptides exhibited greater expression of caspases-3, -8 and -9 than those merely treated with single agent of PTX or RGD peptide. Furthermore, the caspase-3, -8 and -9 inhibitor presented significant protection against E[c(RGDyK)]2 peptide induced U87MG programmed cell death. The increased expression of PTX-induced integrin-αvβ3 was correlated with the enhanced apoptosis in U87MG cells. This study provides a novel concept of targeting integrin-αvβ3 with RGD peptides in combination with low-dose PTX pre-treatment to improve efficiency in human glioblastoma treatment.
Ming-Wei Chang; Jem-Mau Lo; Hsueh-Fen Juan; Hsin-Yi Chang; Chun-Yu Chuang. Combination of RGD Compound and Low-Dose Paclitaxel Induces Apoptosis in Human Glioblastoma Cells. PLoS ONE 2012, 7, e37935 .
AMA StyleMing-Wei Chang, Jem-Mau Lo, Hsueh-Fen Juan, Hsin-Yi Chang, Chun-Yu Chuang. Combination of RGD Compound and Low-Dose Paclitaxel Induces Apoptosis in Human Glioblastoma Cells. PLoS ONE. 2012; 7 (5):e37935.
Chicago/Turabian StyleMing-Wei Chang; Jem-Mau Lo; Hsueh-Fen Juan; Hsin-Yi Chang; Chun-Yu Chuang. 2012. "Combination of RGD Compound and Low-Dose Paclitaxel Induces Apoptosis in Human Glioblastoma Cells." PLoS ONE 7, no. 5: e37935.
Bisphenol A (BPA) is a sealant and flux of plastic materials and has been determined to be an endocrine-disrupting chemical. Prenatal exposure to BPA can lead to substantial adverse effects on fetal growth and development. This study was conducted to assess BPA concentration in pregnant women and umbilical cord blood, and to investigate whether maternal BPA exposure affected fetal outcomes including lower birth weight (LBW), smaller size for gestational age (SGA), and high leptin (HLP) and low adiponectin (LAD) secretion.
Wei-Chun Chou; Jyh-Larng Chen; Chung-Fen Lin; Yi-Chun Chen; Feng-Cheng Shih; Chun-Yu Chuang. Biomonitoring of bisphenol A concentrations in maternal and umbilical cord blood in regard to birth outcomes and adipokine expression: a birth cohort study in Taiwan. Environmental Health 2011, 10, 94 -94.
AMA StyleWei-Chun Chou, Jyh-Larng Chen, Chung-Fen Lin, Yi-Chun Chen, Feng-Cheng Shih, Chun-Yu Chuang. Biomonitoring of bisphenol A concentrations in maternal and umbilical cord blood in regard to birth outcomes and adipokine expression: a birth cohort study in Taiwan. Environmental Health. 2011; 10 (1):94-94.
Chicago/Turabian StyleWei-Chun Chou; Jyh-Larng Chen; Chung-Fen Lin; Yi-Chun Chen; Feng-Cheng Shih; Chun-Yu Chuang. 2011. "Biomonitoring of bisphenol A concentrations in maternal and umbilical cord blood in regard to birth outcomes and adipokine expression: a birth cohort study in Taiwan." Environmental Health 10, no. 1: 94-94.
Diarrhea is an important public health problem in Taiwan. Climatic changes and an increase in extreme weather events (extreme heat, drought or rainfalls) have been strongly linked to the incidence of diarrhea-associated disease. This study investigated and quantified the relationship between climate variations and diarrhea-associated morbidity in subtropical Taiwan. Specifically, this study analyzed the local climatic variables and the number of diarrhea-associated infection cases from 1996 to 2007. This study applied a climate variation-guided Poisson regression model to predict the dynamics of diarrhea-associated morbidity. The proposed model allows for climate factors (relative humidity, maximum temperature and the numbers of extreme rainfall), autoregression, long-term trends and seasonality, and a lag-time effect. Results indicated that the maximum temperature and extreme rainfall days were strongly related to diarrhea-associated morbidity. The impact of maximum temperature on diarrhea-associated morbidity appeared primarily among children (0–14 years) and older adults (40–64 years), and had less of an effect on adults (15–39 years). Otherwise, relative humidity and extreme rainfall days significantly contributed to the diarrhea-associated morbidity in adult. This suggested that children and older adults were the most susceptible to diarrhea-associated morbidity caused by climatic variation. Because climatic variation contributed to diarrhea morbidity in Taiwan, it is necessary to develop an early warning system based on the climatic variation information for disease control management.
Wei-Chun Chou; Jiunn-Lin Wu; Yu-Chun Wang; Hsin Huang; Fung-Chang Sung; Chun-Yu Chuang. Modeling the impact of climate variability on diarrhea-associated diseases in Taiwan (1996–2007). Science of The Total Environment 2010, 409, 43 -51.
AMA StyleWei-Chun Chou, Jiunn-Lin Wu, Yu-Chun Wang, Hsin Huang, Fung-Chang Sung, Chun-Yu Chuang. Modeling the impact of climate variability on diarrhea-associated diseases in Taiwan (1996–2007). Science of The Total Environment. 2010; 409 (1):43-51.
Chicago/Turabian StyleWei-Chun Chou; Jiunn-Lin Wu; Yu-Chun Wang; Hsin Huang; Fung-Chang Sung; Chun-Yu Chuang. 2010. "Modeling the impact of climate variability on diarrhea-associated diseases in Taiwan (1996–2007)." Science of The Total Environment 409, no. 1: 43-51.