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Skeletal muscle plays important roles in animal locomotion, metabolism, and meat production in farm animals. Current studies showed that non-coding RNAs, especially the circular RNA (circRNA) play an indispensable role in skeletal muscle development. Our previous study revealed that several differentially expressed circRNAs among fast muscle growing broilers (FMGB) and slow muscle growing layers (SMGL) may regulate muscle development in the chicken. In this study, a novel differentially expressed circPPP1R13B was identified. Molecular mechanism analysis indicated that circPPP1R13B targets miR-9-5p and negatively regulates the expression of miR-9-5p, which was previously reported to be an inhibitor of skeletal muscle development. In addition, circPPP1R13B positively regulated the expression of miR-9-5p target gene insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) and further activated the downstream insulin like growth factors (IGF)/phosphatidylinositol 3-kinase (PI3K)/AKT serine/threonine kinase (AKT) signaling pathway. The results also showed that the knockdown of circPPP1R13B inhibits chicken skeletal muscle satellite cells (SMSCs) proliferation and differentiation, and the overexpression of circPPP1R13B promotes the proliferation and differentiation of chicken SMSCs. Furthermore, the overexpression of circPPP1R13B could block the inhibitory effect of miR-9-5p on chicken SMSC proliferation and differentiation. In summary, our results suggested that circPPP1R13B promotes chicken SMSC proliferation and differentiation by targeting miR-9-5p and activating IGF/PI3K/AKT signaling pathway.
Xiaoxu Shen; Yuanhang Wei; Guishuang You; Wei Liu; Felix Kwame Amevor; Yao Zhang; Haorong He; Menggen Ma; Yun Zhang; Diyan Li; Qing Zhu; Huadong Yin. Circular PPP1R13B RNA Promotes Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via Targeting miR-9-5p. Animals 2021, 11, 2396 .
AMA StyleXiaoxu Shen, Yuanhang Wei, Guishuang You, Wei Liu, Felix Kwame Amevor, Yao Zhang, Haorong He, Menggen Ma, Yun Zhang, Diyan Li, Qing Zhu, Huadong Yin. Circular PPP1R13B RNA Promotes Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via Targeting miR-9-5p. Animals. 2021; 11 (8):2396.
Chicago/Turabian StyleXiaoxu Shen; Yuanhang Wei; Guishuang You; Wei Liu; Felix Kwame Amevor; Yao Zhang; Haorong He; Menggen Ma; Yun Zhang; Diyan Li; Qing Zhu; Huadong Yin. 2021. "Circular PPP1R13B RNA Promotes Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via Targeting miR-9-5p." Animals 11, no. 8: 2396.
Body weight at the onset of egg production is a major factor influencing hen productivity, as suitable body weight is crucial to laying performance in laying hens. To better understand the association between body weight and microbial community membership and structure in different sites of the digestive and reproductive tracts in chickens, we performed 16S rRNA sequencing surveys and focused on how the microbiota may interact to influence body weight. Our results demonstrated that the microbial community and structure of the digestive and reproductive tracts differed between low and high body weight groups. In particular, we found that the species Pseudomonas viridiflava was negatively associated with body weight in the three digestive tract sites, while Bacteroides salanitronis was negatively associated with body weight in the three reproductive tract sites; and further in-depth studies are needed to explore their function. These findings will help extend our understanding of the influence of the bird digestive and reproductive tract microbiotas on body weight trait and provide future directions regarding the control of body weight in the production of laying hens.
Yuan Su; Yile Ge; Zhongxian Xu; Dejing Zhang; Diyan Li. The digestive and reproductive tract microbiotas and their association with body weight in laying hens. Poultry Science 2021, 101422 .
AMA StyleYuan Su, Yile Ge, Zhongxian Xu, Dejing Zhang, Diyan Li. The digestive and reproductive tract microbiotas and their association with body weight in laying hens. Poultry Science. 2021; ():101422.
Chicago/Turabian StyleYuan Su; Yile Ge; Zhongxian Xu; Dejing Zhang; Diyan Li. 2021. "The digestive and reproductive tract microbiotas and their association with body weight in laying hens." Poultry Science , no. : 101422.
The fertility of female animals is negatively correlated with increasing chronological age. In aging broiler breeder hens, there is a decline in the functionality of the ovary and liver accompanied by hormonal or endocrine changes, a reduction in antioxidant capacity, and a decrease in folliculogenesis. Therefore, improving the reproductive function in aging breeder hens using dietary strategies is of great concern to the poultry breeder. This study evaluated the capacity of dietary quercetin (Q), vitamin E (VE), and their combination (Q + VE) to promote follicle development and attenuate organ inflammation by improving the antioxidant capacity of aging breeder hens. In this study, 400 broiler breeder hens (Tianfu broilers breeder hens, 435 days old) were allotted into four groups (100 birds each) with four replicates each (25 birds each). They were fed diets containing Q (0.4 g/kg), VE (0.2 g/kg), Q + VE (0.4 g/kg + 0.2 g/kg), and a basal diet for 10 weeks. The results showed that Q + VE improved the organ characteristics (p< 0.05), and also that Q + VE showed protective effects on the liver against injury, as well as increasing the antioxidant capacity of the liver, serum, and ovary (p< 0.05). Furthermore, liver lipid synthesis was increased remarkably, as indicated by the changes in triglyceride levels in hens fed Q + VE (p< 0.05). Levels of E2, FSH, and LH, their receptors, and mRNAs related to yolk precursor synthesis were increased by the Q + VE (p< 0.05). Therefore, the combination of quercetin and vitamin E synergistically promotes and regulates the transportation and exchange of synthetic substances among the liver–blood–ovary alliances to ensure the synchronous development and functional coordination between the liver and ovary in aging breeder hens.
Felix Amevor; Zhifu Cui; Xiaxia Du; Zifan Ning; Gang Shu; Ningning Jin; Xun Deng; Yaofu Tian; Zhichao Zhang; Xincheng Kang; Dan Xu; Guishuang You; Yao Zhang; Diyan Li; Yan Wang; Qing Zhu; Xiaoling Zhao. Combination of Quercetin and Vitamin E Supplementation Promotes Yolk Precursor Synthesis and Follicle Development in Aging Breeder Hens via Liver–Blood–Ovary Signal Axis. Animals 2021, 11, 1915 .
AMA StyleFelix Amevor, Zhifu Cui, Xiaxia Du, Zifan Ning, Gang Shu, Ningning Jin, Xun Deng, Yaofu Tian, Zhichao Zhang, Xincheng Kang, Dan Xu, Guishuang You, Yao Zhang, Diyan Li, Yan Wang, Qing Zhu, Xiaoling Zhao. Combination of Quercetin and Vitamin E Supplementation Promotes Yolk Precursor Synthesis and Follicle Development in Aging Breeder Hens via Liver–Blood–Ovary Signal Axis. Animals. 2021; 11 (7):1915.
Chicago/Turabian StyleFelix Amevor; Zhifu Cui; Xiaxia Du; Zifan Ning; Gang Shu; Ningning Jin; Xun Deng; Yaofu Tian; Zhichao Zhang; Xincheng Kang; Dan Xu; Guishuang You; Yao Zhang; Diyan Li; Yan Wang; Qing Zhu; Xiaoling Zhao. 2021. "Combination of Quercetin and Vitamin E Supplementation Promotes Yolk Precursor Synthesis and Follicle Development in Aging Breeder Hens via Liver–Blood–Ovary Signal Axis." Animals 11, no. 7: 1915.
A comprehensive transcriptomic survey of pigs can provide a mechanistic understanding of tissue specialization processes underlying economically valuable traits and accelerate their use as a biomedical model. Here we characterize four transcript types (lncRNAs, TUCPs, miRNAs, and circRNAs) and protein-coding genes in 31 adult pig tissues and two cell lines. We uncover the transcriptomic variability among 47 skeletal muscles, and six adipose depots linked to their different origins, metabolism, cell composition, physical activity, and mitochondrial pathways. We perform comparative analysis of the transcriptomes of seven tissues from pigs and nine other vertebrates to reveal that evolutionary divergence in transcription potentially contributes to lineage-specific biology. Long-range promoter–enhancer interaction analysis in subcutaneous adipose tissues across species suggests evolutionarily stable transcription patterns likely attributable to redundant enhancers buffering gene expression patterns against perturbations, thereby conferring robustness during speciation. This study can facilitate adoption of the pig as a biomedical model for human biology and disease and uncovers the molecular bases of valuable traits.
Long Jin; Qianzi Tang; Silu Hu; Zhongxu Chen; Xuming Zhou; Bo Zeng; Yuhao Wang; Mengnan He; Yan Li; Lixuan Gui; Linyuan Shen; Keren Long; Jideng Ma; Xun Wang; Zhengli Chen; Yanzhi Jiang; Guoqing Tang; Li Zhu; Fei Liu; Bo Zhang; Zhiqing Huang; Guisen Li; Diyan Li; Vadim N. Gladyshev; Jingdong Yin; Yiren Gu; Xuewei Li; Mingzhou Li. A pig BodyMap transcriptome reveals diverse tissue physiologies and evolutionary dynamics of transcription. Nature Communications 2021, 12, 1 -17.
AMA StyleLong Jin, Qianzi Tang, Silu Hu, Zhongxu Chen, Xuming Zhou, Bo Zeng, Yuhao Wang, Mengnan He, Yan Li, Lixuan Gui, Linyuan Shen, Keren Long, Jideng Ma, Xun Wang, Zhengli Chen, Yanzhi Jiang, Guoqing Tang, Li Zhu, Fei Liu, Bo Zhang, Zhiqing Huang, Guisen Li, Diyan Li, Vadim N. Gladyshev, Jingdong Yin, Yiren Gu, Xuewei Li, Mingzhou Li. A pig BodyMap transcriptome reveals diverse tissue physiologies and evolutionary dynamics of transcription. Nature Communications. 2021; 12 (1):1-17.
Chicago/Turabian StyleLong Jin; Qianzi Tang; Silu Hu; Zhongxu Chen; Xuming Zhou; Bo Zeng; Yuhao Wang; Mengnan He; Yan Li; Lixuan Gui; Linyuan Shen; Keren Long; Jideng Ma; Xun Wang; Zhengli Chen; Yanzhi Jiang; Guoqing Tang; Li Zhu; Fei Liu; Bo Zhang; Zhiqing Huang; Guisen Li; Diyan Li; Vadim N. Gladyshev; Jingdong Yin; Yiren Gu; Xuewei Li; Mingzhou Li. 2021. "A pig BodyMap transcriptome reveals diverse tissue physiologies and evolutionary dynamics of transcription." Nature Communications 12, no. 1: 1-17.
Long non-coding RNAs (lncRNAs) and mRNAs are temporally expressed during chicken follicle development. However, follicle transcriptome studies in chickens with timepoints relating to changes in luteinizing hormone (LH) levels are rare. In this study, gene expression in Rohman layers was investigated at three distinct stages of the ovulatory cycle: zeitgeber time 0 (ZT0, 9:00 a.m.), zeitgeber time 12 (ZT12, 9:00 p.m.), and zeitgeber time 20 (ZT20, 5:00 a.m.) representing the early, middle, and LH surge stages, respectively, of the ovulatory cycle. Gene expression profiles were explored during follicle development at ZT0, ZT12, and ZT20 using Ribo-Zero RNA sequencing. The three stages were separated into two major stages, including the pre-LH surge and the LH surge stages. A total of 12,479 mRNAs and 7528 lncRNAs were identified among the three stages, and 4531, 523 differentially expressed genes (DEGs) and 2367, 211 differentially expressed lncRNAs (DELs) were identified in the ZT20 vs. ZT12, and ZT12 vs. ZT0, comparisons. Functional enrichment analysis revealed that genes involved in cell proliferation and metabolism processes (lipid-related) were mainly enriched in the ZT0 and ZT12 stages, respectively, and genes related to oxidative stress, steroids regulation, and inflammatory process were enriched in the ZT20 stage. These findings provide the basis for further investigation of the specific genetic and molecular functions of follicle development in chickens.
Liang Li; Xun Deng; Silu Hu; Zhifu Cui; Zifan Ning; Taotao Gui; Xiaoling Zhao; Diyan Li; Yan Wang; Huadong Yin; Lin Ye; Yaofu Tian; Yao Zhang; Hua Li; Qing Zhu. Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle. Animals 2021, 11, 1533 .
AMA StyleLiang Li, Xun Deng, Silu Hu, Zhifu Cui, Zifan Ning, Taotao Gui, Xiaoling Zhao, Diyan Li, Yan Wang, Huadong Yin, Lin Ye, Yaofu Tian, Yao Zhang, Hua Li, Qing Zhu. Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle. Animals. 2021; 11 (6):1533.
Chicago/Turabian StyleLiang Li; Xun Deng; Silu Hu; Zhifu Cui; Zifan Ning; Taotao Gui; Xiaoling Zhao; Diyan Li; Yan Wang; Huadong Yin; Lin Ye; Yaofu Tian; Yao Zhang; Hua Li; Qing Zhu. 2021. "Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle." Animals 11, no. 6: 1533.
Skeletal muscle satellite cell growth and development is a complicated process driven by multiple genes. The PDZ and LIM domain 5 (PDLIM5) gene has been proven to function in C2C12 myoblast differentiation and is involved in the regulation of skeletal muscle development. The role of PDLIM5 in chicken skeletal muscle satellite cells, however, is unclear. In this study, in order to determine whether the PDLIM5 gene has a function in chicken skeletal muscle satellite cells, we examined the changes in proliferation and differentiation of chicken skeletal muscle satellite cells (SMSCs) after interfering and overexpressing PDLIM5 in cells. In addition, the molecular pathways of the PDLIM5 gene regulating SMSC proliferation and differentiation were analyzed by transcriptome sequencing. Our results show that PDLIM5 can promote the proliferation and differentiation of SMSCs; furthermore, through transcriptome sequencing, it can be found that the differential genes are enriched in the MAPK signaling pathway after knocking down PDLIM5. Finally, it was verified that PDLIM5 played an active role in the proliferation and differentiation of chicken SMSCs by activating the p38-MAPK signaling pathway. These results indicate that PDLIM5 may be involved in the growth and development of chicken skeletal muscle.
Haorong He; Huadong Yin; Xueke Yu; Yao Zhang; Menggen Ma; Diyan Li; Qing Zhu. PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway. Animals 2021, 11, 1016 .
AMA StyleHaorong He, Huadong Yin, Xueke Yu, Yao Zhang, Menggen Ma, Diyan Li, Qing Zhu. PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway. Animals. 2021; 11 (4):1016.
Chicago/Turabian StyleHaorong He; Huadong Yin; Xueke Yu; Yao Zhang; Menggen Ma; Diyan Li; Qing Zhu. 2021. "PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway." Animals 11, no. 4: 1016.
Weaning is the most critical phase in pig production and is generally associated with significant impacts on intestinal morphology, structure, physiology, and immune responses, which can lead to subsequent production inefficiencies such as decreases in growth and intake and increases in morbidity and mortality. In the present study, we attempted to explore the effects of fecal microbiota transplantation (FMT) on the fecal microbiota, fecal metabolites, and transcriptome in the jejunum, colon, liver, spleen, and oral mucosa in piglets with post-weaning diarrhea and to evaluate the therapeutic potential of FMT in piglets with post-weaning diarrhea. We found that FMT partially relieved the symptoms of diarrhea in piglets, and microbiota analysis results indicated that unclassified_f_Prevotellaceae was identified as an FMT-associated bacterial family at 66 day and that the Shannon index in the healthy group at 34, 38, and 66 days were higher than that at 21 day. Functional enrichment analysis of the oral mucosa, liver, jejunum, and colon showed that most of the differentially expressed genes (DEGs) were enriched in the terms metabolic process, immune response, and inflammatory response. Moreover, the enriched fecal metabolites focused mostly on apoptosis, beta-alanine metabolism, glutathione metabolism, and sphingolipid metabolism. We tried to detect specific “metabolite-bacterium” pairs, such as “g_Catenisphaera-stigmastentriol,” “p_Bacteroidetes-(6beta,22E)-6-hydroxystigmasta-4,22-dien-3-one,” and “g_Prevotellaceae_NK3B31_group-stenocereol.” Overall, the present study provides a theoretical basis for the alleviation of weaning stress and contributes to the realization of effective and sustainable application of FMT in the pig production industry in the future.
Yuan Su; Xiaolei Li; Diyan Li; Jing Sun. Fecal Microbiota Transplantation Shows Marked Shifts in the Multi-Omic Profiles of Porcine Post-weaning Diarrhea. Frontiers in Microbiology 2021, 12, 1 .
AMA StyleYuan Su, Xiaolei Li, Diyan Li, Jing Sun. Fecal Microbiota Transplantation Shows Marked Shifts in the Multi-Omic Profiles of Porcine Post-weaning Diarrhea. Frontiers in Microbiology. 2021; 12 ():1.
Chicago/Turabian StyleYuan Su; Xiaolei Li; Diyan Li; Jing Sun. 2021. "Fecal Microbiota Transplantation Shows Marked Shifts in the Multi-Omic Profiles of Porcine Post-weaning Diarrhea." Frontiers in Microbiology 12, no. : 1.
Several reproductive hormones were reported to be involved in regulating egg yolk precursor synthesis in chickens; however, the mechanism that shows how the liver-blood-ovary signal axis works in relation to age changes has not been reported yet. Therefore, in this study, we observe the morphology and histology of the liver and ovary and determine the serum biochemical parameters and the expression abundance of the critical genes from d90 to 153. Results show that the body weight and liver weight were significantly increased from d132, while the ovary weight increased from d139. Aside from the increase in weight, other distinct changes such as the liver color and an increased deposition of large amounts of yolk precursors into the ovarian follicles were observed. On d139, we observed small fatty vacuoles in the hepatocytes. The results of serum biochemical parameters showed a significant increase in the estradiol (E2) level, first on d125, and then it reached its peak on d132. Meanwhile, the levels of follicle-stimulating hormone (FSH) increased initially and then remained at a high level from d146 to d153, while the levels of luteinizing hormone (LH) increased significantly on d132 and reached the top level on d153. Moreover, the levels of lecithin (LEC), vitellogenin (VTG), very low density lipoprotein y (VLDLy), triglyceride (TG), and total cholesterol (TC) were significantly increased at d125 and were close from d146 to d153. The mRNA and protein expression of estrogen receptor-alpha (ER-α) and E2 levels in the liver and serum, respectively, showed similar changes. Moreover, with reference to an increase in serum E2 level, the mRNA expression of genes related to yolk precursor synthesis (very low density apolipoprotein-II, ApoVLDL-II) and vitellogenin-II (VTG-II), lipogenesis (fatty acid synthase, FAS), and lipid transport (microsomal triglyceride transport protein, MTTP) in the liver showed up-regulation. These results suggest that the correlation between liver-blood-ovary alliances regulate the transport and exchange of synthetic substances to ensure synchronous development and functional coordination between the liver and ovary. We also found that E2 is an activator that is regulated by FSH, which induces histological and functional changes in the hepatocytes through the ER-α pathway.
Zhifu Cui; Felix Kwame Amevor; Qian Feng; Xincheng Kang; Weizhen Song; Qing Zhu; Yan Wang; Diyan Li; Xiaoling Zhao. Sexual Maturity Promotes Yolk Precursor Synthesis and Follicle Development in Hens via Liver-Blood-Ovary Signal Axis. Animals 2020, 10, 2348 .
AMA StyleZhifu Cui, Felix Kwame Amevor, Qian Feng, Xincheng Kang, Weizhen Song, Qing Zhu, Yan Wang, Diyan Li, Xiaoling Zhao. Sexual Maturity Promotes Yolk Precursor Synthesis and Follicle Development in Hens via Liver-Blood-Ovary Signal Axis. Animals. 2020; 10 (12):2348.
Chicago/Turabian StyleZhifu Cui; Felix Kwame Amevor; Qian Feng; Xincheng Kang; Weizhen Song; Qing Zhu; Yan Wang; Diyan Li; Xiaoling Zhao. 2020. "Sexual Maturity Promotes Yolk Precursor Synthesis and Follicle Development in Hens via Liver-Blood-Ovary Signal Axis." Animals 10, no. 12: 2348.
The authors wish to make the following corrections to their paper
Jing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84. Animals 2020, 10, 2322 .
AMA StyleJing Zhao, Xiaoxu Shen, Xinao Cao, Haorong He, Shunshun Han, Yuqi Chen, Can Cui, Yuanhang Wei, Yan Wang, Diyan Li, Qing Zhu, Huadong Yin. Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84. Animals. 2020; 10 (12):2322.
Chicago/Turabian StyleJing Zhao; Xiaoxu Shen; Xinao Cao; Haorong He; Shunshun Han; Yuqi Chen; Can Cui; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu; Huadong Yin. 2020. "Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. Animals 2020, 10, 84." Animals 10, no. 12: 2322.
The genetic footprints of adaptations to naturally occurring tropical stress along with domestication are poorly reported in chickens. Here, by conducting population genomic analyses of 67 chickens inhabiting distinct climates, we found signals of gene flow from Tibetan chickens to Sri Lankan and Saudi Arabian breeds and identified 12 positively selected genes that are likely involved in genetic adaptations to both tropical desert and tropical monsoon island climates. Notably, in tropical desert climate, advantageous alleles of TLR7 and ZC3HAV1, which could inhibit replication of viruses in cells, suggest immune adaptation to the defense against zoonotic diseases in chickens. Furthermore, comparative genomic analysis showed that four genes (OC90, PLA2G12B, GPR17 and TNFRSF11A) involved in arachidonic acid metabolism have undergone convergent adaptation to tropical desert climate between birds and mammals. Our study offers insights into the genetic mechanisms of adaptations to tropical climates in birds and other animals and provides practical value for breeding design and medical research on avian viruses.
Shilin Tian; Xuming Zhou; Tashi Phuntsok; Ning Zhao; Dejing Zhang; Chunyou Ning; Diyan Li; Huabin Zhao. Genomic Analyses Reveal Genetic Adaptations to Tropical Climates in Chickens. iScience 2020, 23, 1 .
AMA StyleShilin Tian, Xuming Zhou, Tashi Phuntsok, Ning Zhao, Dejing Zhang, Chunyou Ning, Diyan Li, Huabin Zhao. Genomic Analyses Reveal Genetic Adaptations to Tropical Climates in Chickens. iScience. 2020; 23 (11):1.
Chicago/Turabian StyleShilin Tian; Xuming Zhou; Tashi Phuntsok; Ning Zhao; Dejing Zhang; Chunyou Ning; Diyan Li; Huabin Zhao. 2020. "Genomic Analyses Reveal Genetic Adaptations to Tropical Climates in Chickens." iScience 23, no. 11: 1.
Dulong Chickens (DLCs) live at high altitude (~3000m) and humidity (~90%), which is endemic to Yunnan province, and has gradually developed unique physiological characteristics, but its genetic basis is still unclear. Using the fixation index (FST ) approach, based on whole-genome resequencing, DLCs were analysed to uncover the genomic architecture of the population and candidate genes involved in selection during domestication. A total of 469 candidate genes were obtained to be putatively under selection in DLCs. Further investigations revealed the genic footprint for local adaptation (high-altitude and high-humidity) as the genic signatures that are involved in economic traits (related to egg production). Candidate genes were identified that may be associated with disease resistance, aggressiveness, small body size and positive selection of vision in DLCs. The data revealed the loci of selective signals that operate during selection for production at high altitude and humidity.
Qilin Wang; Diyan Li; Aiwei Guo; Momo Li; L. Li; Jielong Zhou; Shailendra Kumar Mishra; Guangyu Li; Yanqing Duan; Qingqing Li. Whole-genome resequencing of Dulong Chicken reveal signatures of selection. British Poultry Science 2020, 61, 624 -631.
AMA StyleQilin Wang, Diyan Li, Aiwei Guo, Momo Li, L. Li, Jielong Zhou, Shailendra Kumar Mishra, Guangyu Li, Yanqing Duan, Qingqing Li. Whole-genome resequencing of Dulong Chicken reveal signatures of selection. British Poultry Science. 2020; 61 (6):624-631.
Chicago/Turabian StyleQilin Wang; Diyan Li; Aiwei Guo; Momo Li; L. Li; Jielong Zhou; Shailendra Kumar Mishra; Guangyu Li; Yanqing Duan; Qingqing Li. 2020. "Whole-genome resequencing of Dulong Chicken reveal signatures of selection." British Poultry Science 61, no. 6: 624-631.
Fibromodulin (Fmod), which is an extracellular matrix protein, belongs to the extracellular matrix small-leucine-rich proteoglycan family. Fmod is abundantly expressed in muscles and connective tissues and is involved in biological regulation processes, including cell apoptosis, cell adhesion, and modulation of cytokine activity. Fmod is the main regulator of myostatin, which controls the development of muscle cells, but its regulatory path is unknown. Chicken models are ideal for studying embryonic skeletal muscle development; therefore, to investigate the mechanism of Fmod in muscle development, Fmod-silenced and Fmod-overexpressed chicken myoblasts were constructed. The results showed that Fmod plays a positive role in differentiation by detecting the expression of myogenic differentiation markers, immunofluorescence of MyHC protein, and myotube formation in myoblasts. Fmod regulates expression of atrophy-related genes to alleviate muscle atrophy, which was confirmed by histological analysis of breast muscles in Fmod-modulated chicks in vivo. Additionally, genes differentially expressed between Fmod knockdown and normal myoblasts were enriched in the signaling pathway of transforming growth factor β (TGF-β). Both Fmod-silenced and Fmod-overexpressed myoblasts regulated the expression of TGFBR1 and p-Smad3. Thus, Fmod can promote differentiation but not proliferation of myoblasts by regulating the TGF-β signaling pathway, which may serve a function in muscular atrophy.
Huadong Yin; Can Cui; Shunshun Han; Yuqi Chen; Jing Zhao; Haorong He; Diyan Li; Qing Zhu. Fibromodulin Modulates Chicken Skeletal Muscle Development via the Transforming Growth Factor-β Signaling Pathway. Animals 2020, 10, 1477 .
AMA StyleHuadong Yin, Can Cui, Shunshun Han, Yuqi Chen, Jing Zhao, Haorong He, Diyan Li, Qing Zhu. Fibromodulin Modulates Chicken Skeletal Muscle Development via the Transforming Growth Factor-β Signaling Pathway. Animals. 2020; 10 (9):1477.
Chicago/Turabian StyleHuadong Yin; Can Cui; Shunshun Han; Yuqi Chen; Jing Zhao; Haorong He; Diyan Li; Qing Zhu. 2020. "Fibromodulin Modulates Chicken Skeletal Muscle Development via the Transforming Growth Factor-β Signaling Pathway." Animals 10, no. 9: 1477.
Gene differential expression studies can serve to explore and understand the laws and characteristics of animal life activities, and the difference in gene expression between different animal tissues has been well demonstrated and studied. However, for the world-famous rare and protected species giant panda (Ailuropoda melanoleuca), only the transcriptome of the blood and spleen has been reported separately. Here, in order to explore the transcriptome differences between the different tissues of the giant panda, transcriptome profiles of the heart, liver, spleen, lung, and kidney from five captive giant pandas were constructed with Illumina HiSeq 2500 platform. The comparative analysis of the intertissue gene expression patterns was carried out based on the generated RNA sequencing datasets. Analyses of Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction (PPI) network were performed according to the identified differentially expressed genes (DEGs). We generated 194.52 GB clean base data from twenty-five sequencing libraries and identified 18,701 genes, including 3492 novel genes. With corrected p value 2FoldChange| >2, we finally obtained 921, 553, 574, 457, and 638 tissue-specific DEGs in the heart, liver, spleen, lung, and kidney, respectively. In addition, we identified TTN, CAV3, LDB3, TRDN, and ACTN2 in the heart; FGA, AHSG, and SERPINC1 in the liver; CD19, CD79B, and IL21R in the spleen; NKX2-4 and SFTPB in the lung; GC and HRG in the kidney as hub genes in the PPI network. The results of the analyses showed a similar gene expression pattern between the spleen and lung. This study provided for the first time the heart, liver, lung, and kidney’s transcriptome resources of the giant panda, and it provided a valuable resource for further genetic research or other potential research.
Feng Li; Chengdong Wang; Zhongxian Xu; Mingzhou Li; Linhua Deng; Ming Wei; Hemin Zhang; Kai Wu; Ruihong Ning; Diyan Li; Mingyao Yang; Mingwang Zhang; Qingyong Ni; Bo Zeng; Desheng Li; Ying Li. Transcriptome Profiling across Five Tissues of Giant Panda. BioMed Research International 2020, 2020, 1 -13.
AMA StyleFeng Li, Chengdong Wang, Zhongxian Xu, Mingzhou Li, Linhua Deng, Ming Wei, Hemin Zhang, Kai Wu, Ruihong Ning, Diyan Li, Mingyao Yang, Mingwang Zhang, Qingyong Ni, Bo Zeng, Desheng Li, Ying Li. Transcriptome Profiling across Five Tissues of Giant Panda. BioMed Research International. 2020; 2020 ():1-13.
Chicago/Turabian StyleFeng Li; Chengdong Wang; Zhongxian Xu; Mingzhou Li; Linhua Deng; Ming Wei; Hemin Zhang; Kai Wu; Ruihong Ning; Diyan Li; Mingyao Yang; Mingwang Zhang; Qingyong Ni; Bo Zeng; Desheng Li; Ying Li. 2020. "Transcriptome Profiling across Five Tissues of Giant Panda." BioMed Research International 2020, no. : 1-13.
MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that post-transcriptionally regulate expression of their target genes. Emerging evidence demonstrates that miRNAs are important regulators in the development of skeletal muscle satellite cells (SMSCs). Our previous research showed that gga-miR-3525 is differentially expressed in breast muscle of broilers (high growth rate) and layers (low growth rate). In this study, we report a new role for gga-miR-3525 as a myogenic miRNA that regulates skeletal muscle development in chickens. Exogenous increases in the expression of gga-miR-3525 significantly inhibited proliferation and differentiation of SMSCs, whereas the opposite effects were observed in gga-miR-3525 knockdown SMSCs. We confirmed that PDLIM3 (PDZ and LIM domain 3) is a target gene of gga-miR-3525 that can promote proliferation and differentiation of SMSCs. We found that PDLIM3 overexpression elevated the abundance of phosphorylated (p-)p38 protein but that the gga-miR-3525 mimic and p38-MAPK inhibitor (SB203580) weakened the activation of p-p38. Furthermore, treatment with SB203580 reduced the promoting effect of PDLIM3 on SMSC proliferation and differentiation. Overall, our results indicate that gga-miR-3525 regulates the proliferation and differentiation of SMSCs by targeting PDLIM3 via the p38/MAPK signaling pathway in chickens.
Huadong Yin; Jing Zhao; Haorong He; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu. Gga-miR-3525 Targets PDLIM3 through the MAPK Signaling Pathway to Regulate the Proliferation and Differentiation of Skeletal Muscle Satellite Cells. International Journal of Molecular Sciences 2020, 21, 5573 .
AMA StyleHuadong Yin, Jing Zhao, Haorong He, Yuqi Chen, Yan Wang, Diyan Li, Qing Zhu. Gga-miR-3525 Targets PDLIM3 through the MAPK Signaling Pathway to Regulate the Proliferation and Differentiation of Skeletal Muscle Satellite Cells. International Journal of Molecular Sciences. 2020; 21 (15):5573.
Chicago/Turabian StyleHuadong Yin; Jing Zhao; Haorong He; Yuqi Chen; Yan Wang; Diyan Li; Qing Zhu. 2020. "Gga-miR-3525 Targets PDLIM3 through the MAPK Signaling Pathway to Regulate the Proliferation and Differentiation of Skeletal Muscle Satellite Cells." International Journal of Molecular Sciences 21, no. 15: 5573.
MicroRNA (miRNA) is known to be an important regulator of muscle growth and development. The regulation of microRNA on the skeletal muscle phenotype of animals is mainly achieved by regulating the proliferation and differentiation of myoblasts. In this study, we sequenced a total of 60 samples from 15 developing stages of the pectoral muscle and five other tissues at 300 days of Tibetan chicken. We characterized the expression patterns of miRNAs across muscle developmental stages, and found that the chicken growth and development stage was divided into early-embryonic and late-embryonic as well as postnatal stages. We identified 81 and 21 DE-miRNAs by comparing the miRNA profiles of pectoral muscle of three broad periods and different tissues, respectively; and 271 miRNAs showed time-course patterns. Their potential targets were predicted and used for functional enrichment to understand their regulatory functions. Significantly, GgmiRNA-454 is a time-dependent and tissue-differential expression miRNA. In order to elucidate the role of gga-miRNA-454 in the differentiation of myoblasts, we cultured chicken myoblasts in vitro. The results show that although gga-miRNA-454-3p initiates increase and thereafter decrease during the chicken myoblasts differentiation, it had no effect on primary myoblasts proliferation. Furthermore, we confirm that gga-miRNA-454 inhibits myoblast differentiation by targeting the myotube-associated protein SBF2.
Min Chen; Shaolan Zhang; Zhongxian Xu; Jian Gao; Shailendra Kumar Mishra; Qing Zhu; Xiaoling Zhao; Yan Wang; Huadong Yin; Xiaolan Fan; Bo Zeng; Mingyao Yang; Deying Yang; Qingyong Ni; Yan Li; Mingwang Zhang; Diyan Li. MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development. Frontiers in Genetics 2020, 11, 570 .
AMA StyleMin Chen, Shaolan Zhang, Zhongxian Xu, Jian Gao, Shailendra Kumar Mishra, Qing Zhu, Xiaoling Zhao, Yan Wang, Huadong Yin, Xiaolan Fan, Bo Zeng, Mingyao Yang, Deying Yang, Qingyong Ni, Yan Li, Mingwang Zhang, Diyan Li. MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development. Frontiers in Genetics. 2020; 11 ():570.
Chicago/Turabian StyleMin Chen; Shaolan Zhang; Zhongxian Xu; Jian Gao; Shailendra Kumar Mishra; Qing Zhu; Xiaoling Zhao; Yan Wang; Huadong Yin; Xiaolan Fan; Bo Zeng; Mingyao Yang; Deying Yang; Qingyong Ni; Yan Li; Mingwang Zhang; Diyan Li. 2020. "MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development." Frontiers in Genetics 11, no. : 570.
The liver is the major organ of lipid biosynthesis in the chicken. In laying hens, the liver synthesizes most of the yolk precursors and transports them to developing follicles to produce eggs. However, a systematic investigation of the long non-coding RNA (lncRNA) and mRNA transcriptome in liver across developmental stages is needed. Here, we constructed 12 RNA libraries from liver tissue during four developmental stages: juvenile (day 60), sexual maturity (day 133), peak laying (day 220), and broodiness (day 400). A total of 16,930 putative lncRNAs and 18,260 mRNAs were identified. More than half (53.70%) of the lncRNAs were intergenic lncRNAs. The temporal expression pattern showed that lncRNAs were more restricted than mRNAs. We identified numerous differentially expressed lncRNAs and mRNAs by pairwise comparison between the four developmental stages and found that VTG2, RBP, and a novel protein-coding gene were differentially expressed in all stages. Time-series analysis showed that the modules with upregulated genes were involved in lipid metabolism processes. Co-expression networks suggested functional relatedness between mRNAs and lncRNAs; the DE-lncRNAs were mainly involved in lipid biosynthesis and metabolism processes. We showed that the liver transcriptome varies across different developmental stages. Our results improve our understanding of the molecular mechanisms underlying liver development in chickens.
Chunyou Ning; Tianyuan Ma; Silu Hu; Zhongxian Xu; Pu Zhang; Xiaoling Zhao; Yan Wang; Huadong Yin; Yaodong Hu; Xiaolan Fan; Bo Zeng; Mingyao Yang; Deying Yang; Qingyong Ni; Yan Li; Mingwang Zhang; Huailiang Xu; Yongfang Yao; Qing Zhu; Diyan Li. Long Non-coding RNA and mRNA Profile of Liver Tissue During Four Developmental Stages in the Chicken. Frontiers in Genetics 2020, 11, 1 .
AMA StyleChunyou Ning, Tianyuan Ma, Silu Hu, Zhongxian Xu, Pu Zhang, Xiaoling Zhao, Yan Wang, Huadong Yin, Yaodong Hu, Xiaolan Fan, Bo Zeng, Mingyao Yang, Deying Yang, Qingyong Ni, Yan Li, Mingwang Zhang, Huailiang Xu, Yongfang Yao, Qing Zhu, Diyan Li. Long Non-coding RNA and mRNA Profile of Liver Tissue During Four Developmental Stages in the Chicken. Frontiers in Genetics. 2020; 11 ():1.
Chicago/Turabian StyleChunyou Ning; Tianyuan Ma; Silu Hu; Zhongxian Xu; Pu Zhang; Xiaoling Zhao; Yan Wang; Huadong Yin; Yaodong Hu; Xiaolan Fan; Bo Zeng; Mingyao Yang; Deying Yang; Qingyong Ni; Yan Li; Mingwang Zhang; Huailiang Xu; Yongfang Yao; Qing Zhu; Diyan Li. 2020. "Long Non-coding RNA and mRNA Profile of Liver Tissue During Four Developmental Stages in the Chicken." Frontiers in Genetics 11, no. : 1.
The largest muscles in fowl are the pectorals, which provide the power required for birds to fly. Tibetan chickens show specific adaptations to high-altitude conditions, but changes in the muscle transcriptome associated with these adaptations have not been characterized yet. Therefore, in this study, we used next-generation sequencing technologies to generate eight libraries of mRNA sequences for four Tibetan chickens and four Beijing fatty chickens. A comprehensive transcriptome analysis was performed. In the eight samples, 12 333 annotated protein-coding genes were expressed. Among these, 48 differentially expressed genes were found; all of which were upregulated in Tibetan chickens. These differentially expressed genes were mainly involved in kidney morphogenesis, which indicates that hypoxia has an important effect on renal tubule development. Only nine genes were involved in Kyoto Encyclopedia of Genes and Genomes pathways, such as the endocytosis pathway, the MAPK signaling pathway, the calcium signaling pathway and the TGF-beta signaling pathway. The differentially expressed genes identified in this study will be used to facilitate future research into the Tibetan chicken.
Yaodong Hu; Jiayu Su; Liyuan Cheng; Dan Lan; Diyan Li. Pectoral muscle transcriptome analyses reveal high-altitude adaptations in Tibetan chickens. Animal Biology 2020, 70, 385 -400.
AMA StyleYaodong Hu, Jiayu Su, Liyuan Cheng, Dan Lan, Diyan Li. Pectoral muscle transcriptome analyses reveal high-altitude adaptations in Tibetan chickens. Animal Biology. 2020; 70 (4):385-400.
Chicago/Turabian StyleYaodong Hu; Jiayu Su; Liyuan Cheng; Dan Lan; Diyan Li. 2020. "Pectoral muscle transcriptome analyses reveal high-altitude adaptations in Tibetan chickens." Animal Biology 70, no. 4: 385-400.
Background: The microbiota of the digestive and reproductive systems has a prominent role in animal health and performance, but the extent of its contribution is difficult to determine. In chickens, the effect of host genetics on the reproductive and digestive tract microbiota is unclear, and the means by which digestive/reproductive microbiomes help improve egg production in chicken are unknown.Results: To gain insight into this, we examined genomes from 128 chickens reared under identical conditions and described their digestive (crop, gizzard and small intestine) and reproductive tract (vagina, uterus and isthmus) microbiota. Although the diversity, composition and predicted function of the digestive and reproductive tract microbiota exhibited notable microbiota variation substantially between different parts, host genetics had limited effects on the reproductive and digestive tract microbial community. The digestive and reproductive tract microbiota had a significant effect on egg production (accounting for 52.31% - 98.86% of the variance), after correcting for host genetic effects; in particular, the uterus and isthmus microbiota accounted for an average of 93.59% and 98.86%, respectively, of variance in egg production. We further identified four reproductive tract microbial species which were related to immune system, Bacteroides fragilis, Bacteroides salanitronis, Bacteroides barnesiae and Clostridium leptum, that were significantly positively correlated with egg production. Chickens with a lower abundance of these species had produced significantly fewer eggs at 300 days of age (37.13 vs. 113.75) than those with a higher abundance of these microorganisms. These taxa indicate potential roles play in promoting reproductive performance. Especially uterus and isthmus tract microbiota were major factors in regulating the chicken egg production.Conclusions: Host genetics has limited effect on digestive/reproductive microbiome composition. The distinct site-associated chicken microbiome may be determined by the differences of their physical function. These findings may help design strategies for controlling and altering the digestive/reproductive tract microbiota in chickens to improve egg production.
Diyan Li; Shilin Tian; Wei Zhu; Yuan Su; Tao Wang; Tianyuan Ma; Shailendra Kumar Mishra; Lianzhe Shi; Ranlei Wei; Zhongxian Xu; Chunyou Ning; Dejing Zhang; Xuxu Lin; Xiaoling Zhao; Yan Wang; Huadong Yin; Yaodong Hu; Xiaolan Fan; Bo Zeng; Mingyao Yang; Deying Yang; Qingyong Ni; Yan Li; Mingwang Zhang; Yongfang Yao; Huailiang Xu; Mingzhou Li; Qing Zhu. Influence of the digestive/reproductive tract microbiota on chicken egg production beyond host genetics. 2020, 1 .
AMA StyleDiyan Li, Shilin Tian, Wei Zhu, Yuan Su, Tao Wang, Tianyuan Ma, Shailendra Kumar Mishra, Lianzhe Shi, Ranlei Wei, Zhongxian Xu, Chunyou Ning, Dejing Zhang, Xuxu Lin, Xiaoling Zhao, Yan Wang, Huadong Yin, Yaodong Hu, Xiaolan Fan, Bo Zeng, Mingyao Yang, Deying Yang, Qingyong Ni, Yan Li, Mingwang Zhang, Yongfang Yao, Huailiang Xu, Mingzhou Li, Qing Zhu. Influence of the digestive/reproductive tract microbiota on chicken egg production beyond host genetics. . 2020; ():1.
Chicago/Turabian StyleDiyan Li; Shilin Tian; Wei Zhu; Yuan Su; Tao Wang; Tianyuan Ma; Shailendra Kumar Mishra; Lianzhe Shi; Ranlei Wei; Zhongxian Xu; Chunyou Ning; Dejing Zhang; Xuxu Lin; Xiaoling Zhao; Yan Wang; Huadong Yin; Yaodong Hu; Xiaolan Fan; Bo Zeng; Mingyao Yang; Deying Yang; Qingyong Ni; Yan Li; Mingwang Zhang; Yongfang Yao; Huailiang Xu; Mingzhou Li; Qing Zhu. 2020. "Influence of the digestive/reproductive tract microbiota on chicken egg production beyond host genetics." , no. : 1.
Phenotypic plasticity refers to environment-induced phenotypic changes without mutation and is present in all organisms. The role of phenotypic plasticity in organismal adaptations to novel environments has attracted much attention, but its role in readaptations to ancestral environments is understudied. To address this question, we use the reciprocal transplant approach to investigate the multitissue transcriptomes of chickens adapted to the Tibetan Plateau and adjacent lowland. While many genetic transcriptomic changes had occurred in the forward adaptation to the highland, plastic changes largely transform the transcriptomes to the preferred state when Tibetan chickens are brought back to the lowland. The same trend holds for egg hatchability, a key component of the chicken fitness. These findings, along with highly similar patterns in comparable experiments of guppies andEscherichia coli, demonstrate that organisms generally “remember” their ancestral environments via phenotypic plasticity and reveal a mechanism by which past experience affects future evolution.
Wei-Chin Ho; Diyan Li; Qing Zhu; Jianzhi Zhang. Phenotypic plasticity as a long-term memory easing readaptations to ancestral environments. Science Advances 2020, 6, eaba3388 .
AMA StyleWei-Chin Ho, Diyan Li, Qing Zhu, Jianzhi Zhang. Phenotypic plasticity as a long-term memory easing readaptations to ancestral environments. Science Advances. 2020; 6 (21):eaba3388.
Chicago/Turabian StyleWei-Chin Ho; Diyan Li; Qing Zhu; Jianzhi Zhang. 2020. "Phenotypic plasticity as a long-term memory easing readaptations to ancestral environments." Science Advances 6, no. 21: eaba3388.
MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19. We detected 1836 miRNAs, 233 of which were differentially expressed between broilers and layers. In particular, miRNA-200a-3p was significantly more highly expressed in broilers than layers at three time points. In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. The transforming growth factor 2 (TGF-β2) was identified as a target gene of miR-200a-3p, and which turned out to inhibit differentiation and proliferation, and promote apoptosis of SMSCs. Exogenous TGF-β2 increased the abundances of phosphorylated SMAD2 and SMAD3 proteins, and a miR-200a-3p mimic weakened this effect. The TGF-β2 inhibitor treatment reduced the promotional and inhibitory effects of miR-200a-3p on SMSC differentiation and apoptosis, respectively. Our results indicate that miRNAs are abundantly expressed during embryonic skeletal muscle development, and that miR-200a-3p promotes SMSC development by targeting TGF-β2 and regulating the TGF-β2/SMAD signaling pathway.
Huadong Yin; Haorong He; Xiaoxu Shen; Shuyue Tang; Jing Zhao; Xinao Cao; Shunshun Han; Can Cui; Yuqi Chen; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu. MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway. International Journal of Molecular Sciences 2020, 21, 3274 .
AMA StyleHuadong Yin, Haorong He, Xiaoxu Shen, Shuyue Tang, Jing Zhao, Xinao Cao, Shunshun Han, Can Cui, Yuqi Chen, Yuanhang Wei, Yan Wang, Diyan Li, Qing Zhu. MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway. International Journal of Molecular Sciences. 2020; 21 (9):3274.
Chicago/Turabian StyleHuadong Yin; Haorong He; Xiaoxu Shen; Shuyue Tang; Jing Zhao; Xinao Cao; Shunshun Han; Can Cui; Yuqi Chen; Yuanhang Wei; Yan Wang; Diyan Li; Qing Zhu. 2020. "MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway." International Journal of Molecular Sciences 21, no. 9: 3274.