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Considering the role of magnesium in bone metabolism and the increasing relevance of plant-mediated green-synthesis, this work compares the bone cytocompatibility of magnesium hydroxide nanoparticles (NPs) produced by using pure water, Mg(OH)2, or a rosehip (RH) aqueous extract, Mg(OH)2RH. The NPs were evaluated for dose- and time-dependent effects on human osteoblastic and osteoclastic response, due to the direct involvement of the two cell types in bone metabolism. Mg(OH)2 NPs presented nanoplatelet-like morphology (mean diameter ~90 nm) and a crystalline structure (XRD analysis); the RH-mediated synthesis yielded smaller rounded particles (mean diameter <10 nm) with decreased crystallinity. On the ATR–FTIR spectra, both NPs presented the characteristic Mg-OH peaks; Mg(OH)2RH exhibited additional vibration bands associated with the presence of phytochemicals. On osteoblastic cells, NPs did not affect cell growth and morphology but significantly increased alkaline phosphatase (ALP) activity; on osteoclastic cells, particles had little effect in protein content, tartrate-resistant acid phosphatase (TRAP) activity, percentage of multinucleated cells, and cell area. However, compared with Mg(OH)2, Mg(OH)2RH increased osteoblastic differentiation by inducing ALP activity and promoting the expression of Runx2, SP7, Col1a1, and ALP, and had a negative effect on the expression of the osteoclastic genes NFATC1, CA2, and CTSK. These observations suggest the potential usefulness of Mg(OH)2RH NPs in bone regeneration.
Laura Pinho; Thais Garbieri; Liliana Grenho; Marta Alves; Pedro Sousa Gomes; Carlos Santos; Maria Fernandes; Catarina Santos; Bruno Colaço. Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells. Materials 2021, 14, 4172 .
AMA StyleLaura Pinho, Thais Garbieri, Liliana Grenho, Marta Alves, Pedro Sousa Gomes, Carlos Santos, Maria Fernandes, Catarina Santos, Bruno Colaço. Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells. Materials. 2021; 14 (15):4172.
Chicago/Turabian StyleLaura Pinho; Thais Garbieri; Liliana Grenho; Marta Alves; Pedro Sousa Gomes; Carlos Santos; Maria Fernandes; Catarina Santos; Bruno Colaço. 2021. "Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells." Materials 14, no. 15: 4172.
(1) Background: Alveolar bone defects or decreased alveolar bone height and width may have different causes, such as cleft palate. Regenerative procedures in oro-dental defects are challenging due to anatomical factors and the distinct cell populations involved. The iliac crest bone graft remains the gold-standard for cleft palate closure. However, tissue regeneration approaches have been employed and their outcome reviewed, but no conclusions have been made about which one is the gold-standard. (2) Methods: this umbrella review aims to critically appraise the effectiveness of the current approaches in bone defects regeneration in non-syndromic patients with cleft palate. A search was performed in PubMed, Cochrane Library, Scopus, Web of Science and EMBASE databases. (3) Results: Systematic reviews of randomized and non-randomized controlled trials with or without meta-analysis were included. Nine articles were included in the qualitative analysis and five in the quantitative one. The included studies quality was evaluated with AMSTAR2. (4) Conclusions: The use of new regenerative strategies, such as bone morphogenic protein 2, appears to provide similar results regarding bone volume, filling, and height to the standard technique with the iliac crest bone graft.
Inês Francisco; Anabela Paula; Bárbara Oliveiros; Maria Fernandes; Eunice Carrilho; Carlos Marto; Francisco Vale. Regenerative Strategies in Cleft Palate: An Umbrella Review. Bioengineering 2021, 8, 76 .
AMA StyleInês Francisco, Anabela Paula, Bárbara Oliveiros, Maria Fernandes, Eunice Carrilho, Carlos Marto, Francisco Vale. Regenerative Strategies in Cleft Palate: An Umbrella Review. Bioengineering. 2021; 8 (6):76.
Chicago/Turabian StyleInês Francisco; Anabela Paula; Bárbara Oliveiros; Maria Fernandes; Eunice Carrilho; Carlos Marto; Francisco Vale. 2021. "Regenerative Strategies in Cleft Palate: An Umbrella Review." Bioengineering 8, no. 6: 76.
Activation of renin–angiotensin system (RAS) plays a role in bone deterioration associated with bone metabolic disorders, via increased Angiotensin II (AngII) targeting Angiotensin II type 1 receptor/Angiotensin II type 2 receptor (AT1R/AT2R). Despite the wide data availability, the RAS role remains controversial. This study analyzes the feasibility of using the embryonic chick femur organotypic model to address AngII/AT1R/AT2R axis in bone, which is an application not yet considered. Embryonic day-11 femurs were cultured ex vivo for 11 days in three settings: basal conditions, exposure to AngII, and modulation of AngII effects by prior receptor blockade, i.e., AT1R, AT2R, and AT1R + AT2R. Tissue response was evaluated by combining µCT and histological analysis. Basal-cultured femurs expressed components of RAS, namely ACE, AT1R, AT2R, and MasR (qPCR analysis). Bone formation occurred in the diaphyseal region in all conditions. In basal-cultured femurs, AT1R blocking increased Bone Surface/Bone Volume (BS/BV), whereas Bone Volume/Tissue Volume (BV/TV) decreased with AT2R or AT1R + AT2R blockade. Exposure to AngII greatly decreased BV/TV compared to basal conditions. Receptor blockade prior to AngII addition prevented this effect, i.e., AT1R blockade induced BV/TV, whereas blocking AT2R caused lower BV/TV increase but greater BS/BV; AT1R + AT2R blockade also improved BV/TV. Concluding, the embryonic chick femur model was sensitive to three relevant RAS research setups, proving its usefulness to address AngII/AT1R/AT2R axis in bone both in basal and activated conditions.
Thais Garbieri; Victor Martin; Carlos Santos; Pedro Gomes; Maria Fernandes. The Embryonic Chick Femur Organotypic Model as a Tool to Analyze the Angiotensin II Axis on Bone Tissue. Pharmaceuticals 2021, 14, 469 .
AMA StyleThais Garbieri, Victor Martin, Carlos Santos, Pedro Gomes, Maria Fernandes. The Embryonic Chick Femur Organotypic Model as a Tool to Analyze the Angiotensin II Axis on Bone Tissue. Pharmaceuticals. 2021; 14 (5):469.
Chicago/Turabian StyleThais Garbieri; Victor Martin; Carlos Santos; Pedro Gomes; Maria Fernandes. 2021. "The Embryonic Chick Femur Organotypic Model as a Tool to Analyze the Angiotensin II Axis on Bone Tissue." Pharmaceuticals 14, no. 5: 469.
(1) Background: The etiology of orofacial cleft (OC) is not completely known but several genetic and environmental risk factors have been identified. Moreover, a knowledge gap still persists regarding neonatal characteristics. This study evaluated the effect of parental age and mothers’ body mass index on the risk of having an OC child, in a matched year and sex group (cleft/healthy control). Additionally, birth data were analyzed between groups. (2) Methods: 266 individuals born between 1995 to 2015 were evaluated: 133 OC individuals (85 males/48 females) and 133 control (85 males/48 females). A logistic model was used for the independent variables. ANOVA or Kruskal-Wallis tests were used for comparison between the OC phenotypes. (3) Results: Regarding statistically significant parental related factors, the probability of having a cleft child decreases for each maternal year increase (odds ratio = 0.903) and increases for each body mass index unit (kg/m2) increase (odds ratio = 1.14). On the child data birth, for each mass unit (kg) increase, the probability of having a cleft child decrease (odds ratio = 0.435). (4) Conclusions: In this study, only maternal body mass index and maternal age found statistical differences in the risk of having a cleft child. In the children’s initial data, the cleft group found a higher risk of having a lower birth weight but no relation was found regarding length and head circumference.
Inês Francisco; Francisco Caramelo; Maria Fernandes; Francisco Vale. Parental Risk Factors and Child Birth Data in a Matched Year and Sex Group Cleft Population: A Case-Control Study. International Journal of Environmental Research and Public Health 2021, 18, 4615 .
AMA StyleInês Francisco, Francisco Caramelo, Maria Fernandes, Francisco Vale. Parental Risk Factors and Child Birth Data in a Matched Year and Sex Group Cleft Population: A Case-Control Study. International Journal of Environmental Research and Public Health. 2021; 18 (9):4615.
Chicago/Turabian StyleInês Francisco; Francisco Caramelo; Maria Fernandes; Francisco Vale. 2021. "Parental Risk Factors and Child Birth Data in a Matched Year and Sex Group Cleft Population: A Case-Control Study." International Journal of Environmental Research and Public Health 18, no. 9: 4615.
Saliva is widely used for clinical and laboratory analysis. This study proposed to use DNA extracted from saliva for genotyping and pharmacokinetics of piroxicam. A fast and efficient genotyping method was used to determine relevant allelic variants of CYP2C9 (*2 and *3), since genetic factors can influence in non-steroidal anti-inflammatory drugs (NSAIDs) metabolization. DNA Extract All Reagents Kit® was used for DNA extraction and genotyping was performed using TaqMan® GTXpress™ Master Mix, SNP genotyping assays and a Viia7 Real-Time PCR system. Volunteers performed sequential collections of saliva samples before and after taking a single dose of piroxicam (0.25 to 72 h) which were used for pharmacokinetics assays. Piroxicam concentrations were analyzed using LC-MS/MS. Sixty-six percent of volunteers were ancestral homozygous (CYP2C9*1/*1), and 34% showed one or both polymorphisms. Of these 34%, 22 individuals showed CYP2C9*2 polymorphism, 8 CYP2C9*3, and 4 CYP2C9*2/*3. Piroxicam pharmacokinetics were performed in 5 subjects. Areas under the curve (AUC0-t(h*ng/mL)) for CYP2C9*1/*1, *1/*2 and *1/*3 were, respectively, 194.33±70.93, 166 and 303. Maximum concentrations (Cmax(ng/mL)) for these genotypes were respectively 6.46±2.56, 4.3 and 10.2. Saliva sampling was a very effective matrix for both pharmacogenetic and pharmacokinetic tests, ensuring the speed of the procedure and the well-being and agreement of the participants. Once having the knowledge about the slow and fast metabolizers, it is possible to make an adequate prescription in order to avoid the adverse effects of the medication and to guarantee greater analgesic comfort to the patients respectively.
Bruna Bolani; Gabriela Moraes Oliveira; Thiago José Dionísio; Flavio Augusto Cardoso Faria; Maria Helena Raposo Fernandes; Carlos Ferreira Santos; Adriana Maria Calvo. Pharmacogenetic and Pharmacokinetic Assays from Saliva Samples Can Guarantee Personalized Drug Prescription. Brazilian Dental Journal 2021, 32, 3 -8.
AMA StyleBruna Bolani, Gabriela Moraes Oliveira, Thiago José Dionísio, Flavio Augusto Cardoso Faria, Maria Helena Raposo Fernandes, Carlos Ferreira Santos, Adriana Maria Calvo. Pharmacogenetic and Pharmacokinetic Assays from Saliva Samples Can Guarantee Personalized Drug Prescription. Brazilian Dental Journal. 2021; 32 (1):3-8.
Chicago/Turabian StyleBruna Bolani; Gabriela Moraes Oliveira; Thiago José Dionísio; Flavio Augusto Cardoso Faria; Maria Helena Raposo Fernandes; Carlos Ferreira Santos; Adriana Maria Calvo. 2021. "Pharmacogenetic and Pharmacokinetic Assays from Saliva Samples Can Guarantee Personalized Drug Prescription." Brazilian Dental Journal 32, no. 1: 3-8.
Objectives: This study reports the efficacy of two solvent mixtures on the dissolution of gutta-percha and AH Plus sealer, together with the cytotoxicity. Methods: Methyl ethyl ketone (MEK), orange oil, tetrachloroethylene, MEK/tetrachloroethylene (1:1), MEK/orange oil (1:1), and chloroform (control) were tested. Twelve groups (n = 15) of standardized stainless-steel molds filled with softened gutta-percha cones and twelve (n = 15) filled with AH Plus were immersed in the corresponding mixture or individual solvent, in an ultrasonic bath, for either 2 or 5 min. The effect of the solvents was assessed qualitatively by a topographical analysis (scanning electron microscopy) and chemical analysis (Fourier transform infrared spectroscopy), and quantitatively by a weight loss and viscoelastic property (dynamic mechanical analysis) evaluation. The cytotoxicity was assessed on MG63 human osteoblastic cells. Results: The mixtures did not show the formation of new compounds. Both presented significantly higher efficacies compared to their individual solvents, suggesting a synergistic effect. Their dissolution efficacy was similar to that of chloroform, showing high cytocompatibility. Conclusions: The proposed strategy, incorporating ultrasound agitation and profiting from the synergy of adequate solvents, might enhance root canal cleanliness allowing a single-step procedure to dissolve gutta-percha and the sealer remnants, while assuring cytocompatibility with the periapical tissues.
Inês Ferreira; Liliana Grenho; Pedro Gomes; Ana Cristina Braga; Maria Helena Fernandes; Maria Ascensão Lopes; Irene Pina-Vaz. Efficacy and Cytotoxicity of Binary Mixtures as Root Canal Filling Solvents. Materials 2020, 13, 3237 .
AMA StyleInês Ferreira, Liliana Grenho, Pedro Gomes, Ana Cristina Braga, Maria Helena Fernandes, Maria Ascensão Lopes, Irene Pina-Vaz. Efficacy and Cytotoxicity of Binary Mixtures as Root Canal Filling Solvents. Materials. 2020; 13 (14):3237.
Chicago/Turabian StyleInês Ferreira; Liliana Grenho; Pedro Gomes; Ana Cristina Braga; Maria Helena Fernandes; Maria Ascensão Lopes; Irene Pina-Vaz. 2020. "Efficacy and Cytotoxicity of Binary Mixtures as Root Canal Filling Solvents." Materials 13, no. 14: 3237.
The development of biomaterials that mimicking the hydroxyapatite nanoparticles existent in the immature bone tissue is crucial, especially to accelerate the bone remodeling and regeneration. In this work, it was developed for the first time, hydroxyapatite nanoparticles (NPs) incorporating citrate and zinc (cit-Zn-Hap) in their composition towards a one-step hydrothermal procedure. For comparison purposes, hydroxyapatite NPs incorporating only zinc (Zn-Hap) or citrate (cit-Hap), as well as hydroxyapatite without any of these elements (Hap) were synthesised. The physicochemical characterization was carried out reveling that, the presence of zinc on hydroxyapatite (cit-Zn-Hap), reduced the size of nanoparticles, changed the phosphate environment and decreased the surface charge when compared with cit-Hap nanoparticles. The osteogenic potential of cit-Zn-Hap NPs was analysed in human bone marrow-derived stromal cells (BMSCs), in the absence of osteoinductive factors. NPs were internalized by endocytosis appearing trapped in endosomes and lysosomes scattered through the cytoplasm. Exposure to these NPs resulted in a significant induction of ALP activity, extracellular matrix mineralization, and gene expression of early and later osteogenic transcription factors, as well as of osteoblastic markers. The osteoinductive effect might be regulated, at least in part, by the increased signalling through the canonical WNT pathway. Evaluation of the cell behaviour following exposure to Zn-Hap and cit-Hap strongly suggested a synergistic effect of citrate and Zn in cit-Zn-Hap NPs towards the induction of the osteogenic commitment and functionality of BMSCs. These findings will allow the design of new biomimetic hydroxyapatite nanoparticles with great potential for bone regeneration.
Maria H. Fernandes; Marta M. Alves; Mariana Cebotarenco; Isabel A.C. Ribeiro; Liliana Grenho; Pedro S. Gomes; Maria Carmezim; Catarina F. Santos. Citrate zinc hydroxyapatite nanorods with enhanced cytocompatibility and osteogenesis for bone regeneration. Materials Science and Engineering: C 2020, 115, 111147 .
AMA StyleMaria H. Fernandes, Marta M. Alves, Mariana Cebotarenco, Isabel A.C. Ribeiro, Liliana Grenho, Pedro S. Gomes, Maria Carmezim, Catarina F. Santos. Citrate zinc hydroxyapatite nanorods with enhanced cytocompatibility and osteogenesis for bone regeneration. Materials Science and Engineering: C. 2020; 115 ():111147.
Chicago/Turabian StyleMaria H. Fernandes; Marta M. Alves; Mariana Cebotarenco; Isabel A.C. Ribeiro; Liliana Grenho; Pedro S. Gomes; Maria Carmezim; Catarina F. Santos. 2020. "Citrate zinc hydroxyapatite nanorods with enhanced cytocompatibility and osteogenesis for bone regeneration." Materials Science and Engineering: C 115, no. : 111147.
The aim of the present study was to investigate the influence of low-level red (660 nm) and infrared (780 nm) laser with four different radiance exposures on human umbilical vein endothelial cells (HUVECs) in vitro. HUVECs (1.5 × 104) were incubated in 96-well culture plates. The cells were maintained in M199 medium supplemented with 20% fetal bovine serum, 1% antibiotic (penicillin), 1% anti-mycotic (Fungizone), and 1% endothelial cell growth supplement. After centrifugation, irradiations (660/780 nm, 40 mW, 1, 5, 10, and 20 J/cm2, 1 s, 5 s, 10 s, and 20 s, respectively, total energy 0.4 J, 2 J, 4 J, and 8 J, and beam spot size at target 0.04 cm2) were performed at the bottom of Falcon tubes such that the laser beam directly reached the cell without passing through the culture medium. The cells were divided into groups based on radiant exposures. Cell viability and protein concentration were verified after 1, 2, 3, 6, 8, and 10 days. Red laser increased the cell viability and protein concentration in all groups (three-way ANOVA, p < 0.05) beginning on the second day. The greatest peak compared with the control was found when the radiant exposure was 5 J/cm2 and 10 J/cm2. Infrared laser inhibited cell viability and modulated the protein concentration in the cells, with the highest peak protein concentration found on the second day in the group with radiant exposure of 1 J/cm2 and 10 J/cm2 (three-way ANOVA, p < 0.05). Red laser increased the viability and concentration of total proteins in HUVECs, whereas infrared laser had an inhibitory effect on cell viability, while maintaining the total protein concentration similar to that found in the control group.
Stella Maris Lins Terena; Raquel Agnelli Mesquita-Ferrari; Andreia Martinelli De Siqueira Araújo; Kristianne Porta Santos Fernandes; Maria Helena Fernandes. Photobiomodulation alters the viability of HUVECs cells. Lasers in Medical Science 2020, 36, 83 -90.
AMA StyleStella Maris Lins Terena, Raquel Agnelli Mesquita-Ferrari, Andreia Martinelli De Siqueira Araújo, Kristianne Porta Santos Fernandes, Maria Helena Fernandes. Photobiomodulation alters the viability of HUVECs cells. Lasers in Medical Science. 2020; 36 (1):83-90.
Chicago/Turabian StyleStella Maris Lins Terena; Raquel Agnelli Mesquita-Ferrari; Andreia Martinelli De Siqueira Araújo; Kristianne Porta Santos Fernandes; Maria Helena Fernandes. 2020. "Photobiomodulation alters the viability of HUVECs cells." Lasers in Medical Science 36, no. 1: 83-90.
Preservation of the alveolar bone is a determinant in the outcome of orthodontic treatment. Alveolar bone defects or a decrease of their height and width may occur due to common reasons such as inflammation, tooth extraction, or cleft lip and palate. The aim of this systematic review was to investigate and appraise the quality of the most up to date available evidence regarding the applications and effects of platelet-rich fibrin (PRF) in orthodontics. This study was carried out according to preferred reporting items for systematic reviews and meta-analyses guidelines using the following databases: Medline via PubMed, Cochrane Library, Web of Science Core Collection and EMBASE. The qualitative assessment of the included studies was performed using Cochrane Risk of Bias tool and ROBINS-I guidelines. Results: From a total of 489 studies, nine studies were selected. The majority of the included studies demonstrate that autogenous anterior iliac graft with PRF had a higher amount of newly formed bone. Furthermore, this review also suggests that the application of platelet derivatives in the extraction socket can accelerate orthodontic tooth movement. Despite the limitations in the included studies, this systematic review suggested that PRF can improve alveolar cleft reconstruction and orthodontic tooth movement.
Inês Francisco; Maria Helena Fernandes; Francisco Vale. Platelet-Rich Fibrin in Bone Regenerative Strategies in Orthodontics: A Systematic Review. Materials 2020, 13, 1866 .
AMA StyleInês Francisco, Maria Helena Fernandes, Francisco Vale. Platelet-Rich Fibrin in Bone Regenerative Strategies in Orthodontics: A Systematic Review. Materials. 2020; 13 (8):1866.
Chicago/Turabian StyleInês Francisco; Maria Helena Fernandes; Francisco Vale. 2020. "Platelet-Rich Fibrin in Bone Regenerative Strategies in Orthodontics: A Systematic Review." Materials 13, no. 8: 1866.
Driven by the market demand, several synthetic and natural grafts have been proposed during the last years for tendons regeneration. The synthetic grafts, which present a better mechanical performance than the natural ones, usually fail due to the lack of biocompatibility and bioactivity. Thus, chitosan (Cs) was immobilized on polypropylene (PP) surface, previously activated by plasma treatment, in order to improve the fibroblasts' adhesion and proliferation on it. The Orange II dye method and FTIR-ATR analysis proved the successful Cs immobilization on the PP surface. It was observed by SEM and optical profilometry analysis that the Cs concentration increase leads to a surface roughness (Ra value) increase, as well as to water contact angle (C.A.) decrease at least until 2% (w/V) of Cs. Using the 2% (V/V) Cs solution concentration, according to SEM analysis and resazurin assay, the developed functionalization was well succeeded in improving fibroblasts adhesion and proliferation on PP substrates surface over 7 days of culture.
D.S. Morais; B. Ávila; Cláudia Lopes; Miguel Rodrigues; Filipe Vaz; Ana Machado; Maria Helena Fernandes; R.M. Guedes; Maria A. Lopes. Surface functionalization of polypropylene (PP) by chitosan immobilization to enhance human fibroblasts viability. Polymer Testing 2020, 86, 106507 .
AMA StyleD.S. Morais, B. Ávila, Cláudia Lopes, Miguel Rodrigues, Filipe Vaz, Ana Machado, Maria Helena Fernandes, R.M. Guedes, Maria A. Lopes. Surface functionalization of polypropylene (PP) by chitosan immobilization to enhance human fibroblasts viability. Polymer Testing. 2020; 86 ():106507.
Chicago/Turabian StyleD.S. Morais; B. Ávila; Cláudia Lopes; Miguel Rodrigues; Filipe Vaz; Ana Machado; Maria Helena Fernandes; R.M. Guedes; Maria A. Lopes. 2020. "Surface functionalization of polypropylene (PP) by chitosan immobilization to enhance human fibroblasts viability." Polymer Testing 86, no. : 106507.
Herein the quantitative synthesis of eight new mono- and dianionic Organic Salts and Ionic Liquids (OSILs) from alendronic acid (ALN) is reported by following two distinct sustainable and straightforward methodologies, according to the type of cation. The prepared ALN-OSILs were characterized by spectroscopic techniques and their solubility in water and biological fluids was determined. An evaluation of the toxicity towards human healthy cells and also human breast, lung and bone (osteosarcoma) cell lines was performed. Globally, it was observed that the monoanionic OSILs showed lower toxicity than the corresponding dianionic structures to all cell types. The highest cytotoxic effect was observed in OSILs containing a [C2OHMIM] cation, in particular [C2OHMIM][ALN]. The latter showed an improvement in IC50 values of ca. three orders of magnitude for the lung and bone cancer cell lines as well as fibroblasts in comparison with ALN. The development of OSILs with high cytotoxicity effect towards the tested cancer cell types, and containing an anti-resorbing molecule such as ALN may represent a promising strategy for the development of new pharmacological tools to be used in those pathological conditions.
Sónia Teixeira; Miguel M. Santos; Maria H. Fernandes; João Costa-Rodrigues; Luís C. Branco. Alendronic Acid as Ionic Liquid: New Perspective on Osteosarcoma. Pharmaceutics 2020, 12, 293 .
AMA StyleSónia Teixeira, Miguel M. Santos, Maria H. Fernandes, João Costa-Rodrigues, Luís C. Branco. Alendronic Acid as Ionic Liquid: New Perspective on Osteosarcoma. Pharmaceutics. 2020; 12 (3):293.
Chicago/Turabian StyleSónia Teixeira; Miguel M. Santos; Maria H. Fernandes; João Costa-Rodrigues; Luís C. Branco. 2020. "Alendronic Acid as Ionic Liquid: New Perspective on Osteosarcoma." Pharmaceutics 12, no. 3: 293.
An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control. Bacteriophages were efficiently encapsulated, without jeopardizing phage viability and functionality, nor affecting hydrogel morphology and chemical composition. Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile. Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology. A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite. Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues. This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.
Joana Barros; Luís Melo; Rita Araújo Reis da Silva; Maria Pia Ferraz; Joana Cecília Valente De Rodrigues Azeredo; Victor Pinheiro; Bruno Jorge Antunes Colaço; Maria Helena Fernandes; Pedro Gomes; Fernando Monteiro. Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections. Nanomedicine: Nanotechnology, Biology and Medicine 2020, 24, 102145 .
AMA StyleJoana Barros, Luís Melo, Rita Araújo Reis da Silva, Maria Pia Ferraz, Joana Cecília Valente De Rodrigues Azeredo, Victor Pinheiro, Bruno Jorge Antunes Colaço, Maria Helena Fernandes, Pedro Gomes, Fernando Monteiro. Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections. Nanomedicine: Nanotechnology, Biology and Medicine. 2020; 24 ():102145.
Chicago/Turabian StyleJoana Barros; Luís Melo; Rita Araújo Reis da Silva; Maria Pia Ferraz; Joana Cecília Valente De Rodrigues Azeredo; Victor Pinheiro; Bruno Jorge Antunes Colaço; Maria Helena Fernandes; Pedro Gomes; Fernando Monteiro. 2020. "Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections." Nanomedicine: Nanotechnology, Biology and Medicine 24, no. : 102145.
The clinical demand for bone scaffolds as an alternative strategy for bone grafting has increased exponentially and, up to date, numerous formulations have been proposed to regenerate the bone tissue. However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.). In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response. This innovative combination was designed to improve the scaffolds' functionality, in which GA improved chitosan mechanical strength and stability, whereas CaP granules enhanced the scaffolds' bioactivity and osteoblastic response, further reinforcing the scaffolds' structure. The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance. In addition, the inclusion of biphasic granules induced a trend for increase osteogenic activation, as compared to the addition of HA granules. In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.
Rosana V. Pinto; Pedro S. Gomes; Maria Helena Fernandes; Maria Elizabete Costa; Margarida Almeida. Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications. Materials Science and Engineering: C 2019, 109, 110557 .
AMA StyleRosana V. Pinto, Pedro S. Gomes, Maria Helena Fernandes, Maria Elizabete Costa, Margarida Almeida. Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications. Materials Science and Engineering: C. 2019; 109 ():110557.
Chicago/Turabian StyleRosana V. Pinto; Pedro S. Gomes; Maria Helena Fernandes; Maria Elizabete Costa; Margarida Almeida. 2019. "Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications." Materials Science and Engineering: C 109, no. : 110557.
Periodontal diseases remain a challenge due to a complex interplay of factors involving a chronic inflammatory activation and bacteria internalization in periodontal cells. In this work, chitosan-nanoparticles loaded with minocycline (MH-NPs), a tetracycline with antimicrobial and anti-inflammatory effects, were developed for in situ delivery in the periodontal milieu aiming to improve drug effectiveness. A general cytocompatibility evaluation and a detailed approach to address the cellular uptake process, trafficking pathways and the modulation of relevant inflammatory gene expression was conducted using human gingival fibroblasts. Results show that MH-NPs with an adequate cytocompatible profile can be internalized by distinct endocytic processes (macropinocytosis and clathrin-mediated endocytosis). The ability to modulate autophagy with the delivery within the same endosomal/lysosomal pathway as periodontal pathogens was observed, which increases the intracellular drug effectiveness. Porphyromonas gingivalis LPS-stimulated cultures, grown in the presence of MH-NPs, were found to express significantly reduced levels of inflammation-related markers (IL-1b, TNFα, CXCL-8, NFKB1). These nanoparticles can be potentially used in periodontal disease treatment conjoining the ability of intracellular drug targeting with significant anti-inflammatory effects.
Victor Martin; Isabel A.C. Ribeiro; Marta M. Alves; Lídia Gonçalves; António Almeida; Liliana Grenho; Maria Helena Fernandes; Catarina F. Santos; Pedro S. Gomes; Ana F. Bettencourt. Understanding intracellular trafficking and anti-inflammatory effects of minocycline chitosan-nanoparticles in human gingival fibroblasts for periodontal disease treatment. International Journal of Pharmaceutics 2019, 572, 118821 .
AMA StyleVictor Martin, Isabel A.C. Ribeiro, Marta M. Alves, Lídia Gonçalves, António Almeida, Liliana Grenho, Maria Helena Fernandes, Catarina F. Santos, Pedro S. Gomes, Ana F. Bettencourt. Understanding intracellular trafficking and anti-inflammatory effects of minocycline chitosan-nanoparticles in human gingival fibroblasts for periodontal disease treatment. International Journal of Pharmaceutics. 2019; 572 ():118821.
Chicago/Turabian StyleVictor Martin; Isabel A.C. Ribeiro; Marta M. Alves; Lídia Gonçalves; António Almeida; Liliana Grenho; Maria Helena Fernandes; Catarina F. Santos; Pedro S. Gomes; Ana F. Bettencourt. 2019. "Understanding intracellular trafficking and anti-inflammatory effects of minocycline chitosan-nanoparticles in human gingival fibroblasts for periodontal disease treatment." International Journal of Pharmaceutics 572, no. : 118821.
Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability. However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite. Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration. The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects. The main morphological characteristics of the alginate network were not affected by the addition of nanohydroxyapatite. However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent. Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response. Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response. The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
J. Barros; Maria Pia Ferraz; J. Azeredo; Maria Helena Fernandes; P.S. Gomes; F.J. Monteiro. Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration. Materials Science and Engineering: C 2019, 105, 109985 .
AMA StyleJ. Barros, Maria Pia Ferraz, J. Azeredo, Maria Helena Fernandes, P.S. Gomes, F.J. Monteiro. Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration. Materials Science and Engineering: C. 2019; 105 ():109985.
Chicago/Turabian StyleJ. Barros; Maria Pia Ferraz; J. Azeredo; Maria Helena Fernandes; P.S. Gomes; F.J. Monteiro. 2019. "Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration." Materials Science and Engineering: C 105, no. : 109985.
Scaffolds based on aligned and non-aligned poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibers obtained by electrospinning, associated to electrosprayed hydroxyapatite (HA) for tissue engineering applications were developed and their performance was compared in terms of their morphology and biological and mechanical behaviors. The morphological results assessed by scanning electron microscopy showed a mesh of PLLA/PCL fibers (random and perfectly aligned) associated with aggregates of nanophased HA. Fourier transform infrared spectrometry confirmed the homogeneity in the blends and the presence of nanoHA in the scaffold. As a result of fiber alignment a 15-fold increase in Young’s Modulus and an 8-fold increase in tensile strength were observed when compared to non-aligned fibers. In PLLA/PCL/HA scaffolds, the introduction of nanoHA caused a remarkable improvement of the mechanical strength of this material acting as a reinforcement, enhancing the response of these constructs to tensile stress. In vitro testing was evaluated using osteoblast (MC3T3-E1) cells. The results showed that both fibrous scaffolds were able to support osteoblast cell adhesion and proliferation and that fiber alignment induced increased cellular metabolic activity. In addition, the adhesion and proliferation of Staphylococcus aureus were evaluated and a lower number of colony forming units (CFUs) was obtained in the scaffolds with aligned fibers.
Lilian De Siqueira; Nilza Ribeiro; Maria B. A. Paredes; Liliana Grenho; Cassilda Cunha-Reis; Eliandra S. Trichês; Maria H. Fernandes; Susana R. Sousa; Fernando J. Monteiro. Influence of PLLA/PCL/HA Scaffold Fiber Orientation on Mechanical Properties and Osteoblast Behavior. Materials 2019, 12, 3879 .
AMA StyleLilian De Siqueira, Nilza Ribeiro, Maria B. A. Paredes, Liliana Grenho, Cassilda Cunha-Reis, Eliandra S. Trichês, Maria H. Fernandes, Susana R. Sousa, Fernando J. Monteiro. Influence of PLLA/PCL/HA Scaffold Fiber Orientation on Mechanical Properties and Osteoblast Behavior. Materials. 2019; 12 (23):3879.
Chicago/Turabian StyleLilian De Siqueira; Nilza Ribeiro; Maria B. A. Paredes; Liliana Grenho; Cassilda Cunha-Reis; Eliandra S. Trichês; Maria H. Fernandes; Susana R. Sousa; Fernando J. Monteiro. 2019. "Influence of PLLA/PCL/HA Scaffold Fiber Orientation on Mechanical Properties and Osteoblast Behavior." Materials 12, no. 23: 3879.
Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely (Z)-5-amino-1-methyl-N′-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. To address these goals, both quantitative methods (by cultivable cell numbers) and qualitative evaluation (by scanning electron microscopy) were used. Compounds cytocompatibility towards osteoblast-like cells was also evaluated after 24 h of exposure, through resazurin assay. The three tested compounds displayed a strong inhibitory effect on biofilm development of both Candida species as potent in vitro activity against C. albicans sessile cells. Regarding cytocompatibility, a concentration-dependent effect was observed. Together, these findings indicated that the potent activity of imidazole derivatives on Candida spp. biofilms on nanohydroxyapatite substrate, in particular compound 1c, is worth further investigating.
C. Gabriel; Liliana Grenho; Fátima Cerqueira; Rui Medeiros; Alice Dias; Ana Ribeiro; Fernanda Proença; Maria Helena Fernandes; J. C. Sousa; Fernando Monteiro; Maria Pia Ferraz. Inhibitory Effect of 5-Aminoimidazole-4-Carbohydrazonamides Derivatives Against Candida spp. Biofilm on Nanohydroxyapatite Substrate. Mycopathologia 2019, 184, 775 -786.
AMA StyleC. Gabriel, Liliana Grenho, Fátima Cerqueira, Rui Medeiros, Alice Dias, Ana Ribeiro, Fernanda Proença, Maria Helena Fernandes, J. C. Sousa, Fernando Monteiro, Maria Pia Ferraz. Inhibitory Effect of 5-Aminoimidazole-4-Carbohydrazonamides Derivatives Against Candida spp. Biofilm on Nanohydroxyapatite Substrate. Mycopathologia. 2019; 184 (6):775-786.
Chicago/Turabian StyleC. Gabriel; Liliana Grenho; Fátima Cerqueira; Rui Medeiros; Alice Dias; Ana Ribeiro; Fernanda Proença; Maria Helena Fernandes; J. C. Sousa; Fernando Monteiro; Maria Pia Ferraz. 2019. "Inhibitory Effect of 5-Aminoimidazole-4-Carbohydrazonamides Derivatives Against Candida spp. Biofilm on Nanohydroxyapatite Substrate." Mycopathologia 184, no. 6: 775-786.
Novel ionic liquids and organic salts based on mono‐ or dianionic zoledronate and protonated superbases, choline and n‐alkylmethylimidazolium cations, were prepared and characterized by spectroscopic and thermal analyses. Most of the prepared salts display amorphous structures and very high solubility in water and saline solutions, especially the dianionic salts. Among the zoledronate‐based ionic compounds, those containing choline [Ch] and methoxyethylmethylimidazolium [C3OMIM] cations appear to have significant cytotoxicity against human osteosarcoma cells (MG63) and low toxicity toward healthy skin fibroblast cells. Because osteosarcoma is a bone pathology characterized by an increase in bone turnover rate, the results presented herein may be a promising starting point for the development of new ionic pharmaceutical drugs against osteosarcoma.
Sónia Teixeira; Miguel M. Santos; Ricardo Ferraz; Cristina Prudêncio; Maria H. Fernandes; João Costa‐Rodrigues; Luís C. Branco. A Novel Approach for Bisphosphonates: Ionic Liquids and Organic Salts from Zoledronic Acid. ChemMedChem 2019, 14, 1767 -1770.
AMA StyleSónia Teixeira, Miguel M. Santos, Ricardo Ferraz, Cristina Prudêncio, Maria H. Fernandes, João Costa‐Rodrigues, Luís C. Branco. A Novel Approach for Bisphosphonates: Ionic Liquids and Organic Salts from Zoledronic Acid. ChemMedChem. 2019; 14 (20):1767-1770.
Chicago/Turabian StyleSónia Teixeira; Miguel M. Santos; Ricardo Ferraz; Cristina Prudêncio; Maria H. Fernandes; João Costa‐Rodrigues; Luís C. Branco. 2019. "A Novel Approach for Bisphosphonates: Ionic Liquids and Organic Salts from Zoledronic Acid." ChemMedChem 14, no. 20: 1767-1770.
The continuous need for high-performance implants that can withstand mechanical loads while promoting implant integration into bone has focused recent research on the surface modification of hard ceramics. Their properties of biocompatibility, high mechanical and fatigue resistance and aesthetic color have contributed to its succefull applications in dentistry. Alumina toughened Zirconia (ATZ) has been gaining attention as a material for dental implants applications due to its advanced mechanical properties and minimal degradation at body temperature. Still, in order to improve tissue response to this bioinert material, additional modifications are desirable. Improving the surface functionality of this ceramic could lead to enhanced implant-tissue interaction and subsequently, a successful implant integration. In this work, microtopographies were developed on the surface of Alumina toughened Zirconia using an ultrafast laser methodology, aiming at improving the cellular response to this ceramic. Microscale grooves and grid-like geometries were produced on ATZ ceramics by femtosecond laser ablation, with a pulse width of 150 fs, wavelength of 800 nm and repetition rate of 1 kHz. The variation of surface topography, roughness, chemistry and wettability with different laser processing parameters was examined. Cell-surface interactions were evaluated for 7 days on both microstructured surfaces and a non-treated control with pre-osteoblasts, MC3T3-E1 cells. Both surface topographies showed to improve cell response, with increased metabolic activity when compared to the untreated control and modulating cell morphology up to 7 days. The obtained results suggest that femtosecond laser texturing may be a suitable non-contact methodology for creating tunable micro-scale surface topography on ATZ ceramics to enhance the biological response.
Angela Carvalho; Liliana Grenho; Maria Helena Fernandes; Albena Daskalova; Anton Trifonov; Ivan Buchvarov; Fernando Monteiro. Femtosecond laser microstructuring of alumina toughened zirconia for surface functionalization of dental implants. Ceramics International 2019, 46, 1383 -1389.
AMA StyleAngela Carvalho, Liliana Grenho, Maria Helena Fernandes, Albena Daskalova, Anton Trifonov, Ivan Buchvarov, Fernando Monteiro. Femtosecond laser microstructuring of alumina toughened zirconia for surface functionalization of dental implants. Ceramics International. 2019; 46 (2):1383-1389.
Chicago/Turabian StyleAngela Carvalho; Liliana Grenho; Maria Helena Fernandes; Albena Daskalova; Anton Trifonov; Ivan Buchvarov; Fernando Monteiro. 2019. "Femtosecond laser microstructuring of alumina toughened zirconia for surface functionalization of dental implants." Ceramics International 46, no. 2: 1383-1389.
The task of Group I was to review and update the existing data concerning the physiologic process of socket healing, in the absence or presence of grafting materials or platelet concentrates, addressing the associated molecular and cellular events that culminate in the restoration of the lost tissue architecture and functionality. The second task was to review current literature concerning extraction socket classification immediately following tooth extraction and the rationales for socket preservation/augmentation procedures and with reference to it suggest novel clinical decision tree for extraction socket preservation/augmentation in aesthetic and non-aesthetic area. The main areas indicated by this group were as follows: socket healing process, including haemostasis and coagulation, inflammatory phase, proliferative phase, bone tissue modelling and remodelling; socket healing with graft materials and autologous platelet concentrates; extraction socket classifications; indications and reasons for extraction socket preservation/augmentation. The systematic reviews and/or meta-analyses were registered in PROSPERO, an international prospective register of systematic reviews: http://www.crd.york.ac.uk/PROSPERO/. The literature in the corresponding areas of interest was screened and reported following the PRISMA (Preferred Reporting Item for Systematic Review and Meta-Analysis) Statement: http://www.prisma-statement.org/. Method of preparation of the systematic reviews, based on comprehensive search strategies, was discussed and standardized. The summary of the materials and methods employed by the authors in preparing the systematic reviews and/or meta-analyses is presented in Preface chapter. The results and conclusions of the review process are presented in the respective papers. One theoretical review-analysis and one systematic review were performed. The group's general commentaries, consensus statements, clinical recommendations and implications for research are presented in this article.
Gintaras Juodzbalys; Povilas Daugela; Onurcem Duruel; Maria Helena Fernandes; Pedro De Sousa Gomes; Samir Goyushov; Lorena Mariano; Lukas Poskevicius; Arturas Stumbras; Tolga Fikret Tözüm. The 2nd Baltic Osseointegration Academy and Lithuanian University of Health Sciences Consensus Conference 2019. Summary and Consensus Statements: Group I - Biological Aspects of Tooth Extraction, Socket Healing and Indications for Socket Preservation. Journal of Oral and Maxillofacial Research 2019, 10, e4 .
AMA StyleGintaras Juodzbalys, Povilas Daugela, Onurcem Duruel, Maria Helena Fernandes, Pedro De Sousa Gomes, Samir Goyushov, Lorena Mariano, Lukas Poskevicius, Arturas Stumbras, Tolga Fikret Tözüm. The 2nd Baltic Osseointegration Academy and Lithuanian University of Health Sciences Consensus Conference 2019. Summary and Consensus Statements: Group I - Biological Aspects of Tooth Extraction, Socket Healing and Indications for Socket Preservation. Journal of Oral and Maxillofacial Research. 2019; 10 (3):e4.
Chicago/Turabian StyleGintaras Juodzbalys; Povilas Daugela; Onurcem Duruel; Maria Helena Fernandes; Pedro De Sousa Gomes; Samir Goyushov; Lorena Mariano; Lukas Poskevicius; Arturas Stumbras; Tolga Fikret Tözüm. 2019. "The 2nd Baltic Osseointegration Academy and Lithuanian University of Health Sciences Consensus Conference 2019. Summary and Consensus Statements: Group I - Biological Aspects of Tooth Extraction, Socket Healing and Indications for Socket Preservation." Journal of Oral and Maxillofacial Research 10, no. 3: e4.