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The emergence and worldwide spread of carbapenemase-producing Enterobacterales (CPE) is a great public-health concern. This study aimed to screen for the presence of CPE isolates from Barbary deer in Akfadou Forest, Béjaïa (Algeria). Faecal samples (n = 39) were obtained from Barbary deer in Akfadou Forest between March–June 2018. Whole-genome sequencing (WGS) was performed to characterise one representative strain of Klebsiella pneumoniae. Data analysis was performed using online tools. A total of 13 carbapenem-resistantK. pneumoniae isolates were obtained. The isolates showed an identical antimicrobial resistance pattern and were susceptible to colistin and fosfomycin. WGS analysis revealed the complete resistome of K. pneumoniae strain CF21, including blaNDM-1, blaCTX-M-15, blaSHV-182, blaDHA-1, blaOXA-1, aac(3)-IIa, aac(3)-IId, aac(6ʹ)-Ib-cr, rmtC, sul1, qnrB9, fosA, tetA, dfrA14, catA2, catB3 and mphA. Multilocus sequence typing (MLST) analysis assigned this strain to the international clone ST11. Plasmid analysis showed that this K. pneumoniae strain possesses five different plasmids including IncA/C2, IncFIA(HI1), IncFIB(K), IncFII(K) and ColRNAI. This study reports a multidrug-resistantK. pneumoniae strain recovered from Barbary deer in Algeria and confirms that wild animals could serve as a reservoir of antimicrobial resistance genes.
Assia Mairi; Olivier Barraud; Anaelle Muggeo; Christophe de Champs; Abdelaziz Touati. Genomic analysis of a multidrug-resistant Klebsiella pneumoniae ST11 strain recovered from Barbary deer (Cervus elaphus barbarus) in Akfadou Forest, Algeria. Journal of Global Antimicrobial Resistance 2020, 22, 515 -518.
AMA StyleAssia Mairi, Olivier Barraud, Anaelle Muggeo, Christophe de Champs, Abdelaziz Touati. Genomic analysis of a multidrug-resistant Klebsiella pneumoniae ST11 strain recovered from Barbary deer (Cervus elaphus barbarus) in Akfadou Forest, Algeria. Journal of Global Antimicrobial Resistance. 2020; 22 ():515-518.
Chicago/Turabian StyleAssia Mairi; Olivier Barraud; Anaelle Muggeo; Christophe de Champs; Abdelaziz Touati. 2020. "Genomic analysis of a multidrug-resistant Klebsiella pneumoniae ST11 strain recovered from Barbary deer (Cervus elaphus barbarus) in Akfadou Forest, Algeria." Journal of Global Antimicrobial Resistance 22, no. : 515-518.
Introduction: The Middle East is actually recognized as endemic for carbapenemases-producing Enterobacterales (CPE) including at least OXA-48-like and NDM-like. Areas covered: We performed a search of PubMed and Scopus using relevant keywords. We included peer-reviewed articles published only in English reporting any data on carbapenemase-producing bacteria from Middle East countries. The last literature search was performed on 26 October 2019. All studies describing carbapenemase-producing Enterobacterales isolated from humans, animals or environmental samples from the Middle East were included. Expert opinion: The Middle-East is considered an endemic region for CPE strains and the extensive international exchange could facilitate the spread of CPE from these countries to other parts of the Globe in which the prevalence of the CPE is low. The expansion of the Middle East conflict has been associated with the rapid collapse of the existing health care system of the concerned countries. Considering that Millions of refugees have fled their country, they could introduce these CPE strains in countries with low endemicity. In conclusion, the health care system actors should take in a count the endemicity of CPE in these countries and develop local surveillance programs to limit the spread of these MDR bacteria.
Abdelaziz Touati; Assia Mairi. Epidemiology of carbapenemase-producing Enterobacterales in the Middle East: a systematic review. Expert Review of Anti-infective Therapy 2020, 18, 241 -250.
AMA StyleAbdelaziz Touati, Assia Mairi. Epidemiology of carbapenemase-producing Enterobacterales in the Middle East: a systematic review. Expert Review of Anti-infective Therapy. 2020; 18 (3):241-250.
Chicago/Turabian StyleAbdelaziz Touati; Assia Mairi. 2020. "Epidemiology of carbapenemase-producing Enterobacterales in the Middle East: a systematic review." Expert Review of Anti-infective Therapy 18, no. 3: 241-250.
This review assessed the molecular characterization of the methicillin-resistant Staphylococcus aureus (MRSA)-ST80 clone with an emphasis on its proportion of total MRSA strains isolated, PVL production, spa-typing, antibiotic resistance, and virulence. A systematic review of the literature was conducted on MRSA-ST80 clone published between 1 January 2000 and 31 August 2019. Citations were chosen for a review of the full text if we found evidence that MRSA-ST80 clone was reported in the study. For each isolate, the country of isolation, the sampling period, the source of isolation (the type of infection, nasal swabs, or extra-human), the total number of MRSA strains isolated, number of MRSA-ST80 strains, antibiotic resistance patterns, PVL production, virulence genes, and spa type were recorded. The data from 103 articles were abstracted into an Excel database. Analysis of the data showed that the overall proportion of MRSA-ST80 has been decreasing in many countries in recent years. The majority of MRSA-ST80 were PVL positive with spa-type t044. Only six reports of MRSA-ST80 in extra-human niches were found. This review summarizes the rise of MRSA-ST80 and the evidence that suggests that it could be in decline in many countries.
Assia Mairi; Abdelaziz Touati; Jean-Philippe Lavigne. Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review. Toxins 2020, 12, 119 .
AMA StyleAssia Mairi, Abdelaziz Touati, Jean-Philippe Lavigne. Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review. Toxins. 2020; 12 (2):119.
Chicago/Turabian StyleAssia Mairi; Abdelaziz Touati; Jean-Philippe Lavigne. 2020. "Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review." Toxins 12, no. 2: 119.
The diffusion of Panton-Valentine leukocidin (PVL)-positive methicillin-resistant S. aureus (MRSA) is a health problem in Algeria. The objectives of the study were to investigate the global distribution of methicillin-susceptible S. aureus (MSSA) and MRSA isolates in different ecological niches in this country. In total, 2246 samples were collected from humans, livestock, wild animals, pets, food products and the aquatic environment, from 12 Algerian provinces. A total of 312 S. aureus were detected from 2446 samples (12.7%) in the screened niches. We observed the emergence of toxinogenic S. aureus representing 41% of the isolates. Among them, we noted the diffusion of ST80-IV CA-MRSA PVL + strains isolated in human, animals, and food and genetic diversity of MSSA PVL + isolates. This study suggests an alarming dissemination of MRSA-ST80 PVL + in both human and extra-human sources in Algeria. Moreover, MSSA may become a permanent reservoir of the PVL genes necessary for human infections.
Assia Mairi; Abdelaziz Touati; Alix Pantel; Karima Zenati; Alex Yahiaoui Martinez; Catherine Dunyach-Remy; Albert Sotto; Jean-Philippe Lavigne. Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria. Toxins 2019, 11, 500 .
AMA StyleAssia Mairi, Abdelaziz Touati, Alix Pantel, Karima Zenati, Alex Yahiaoui Martinez, Catherine Dunyach-Remy, Albert Sotto, Jean-Philippe Lavigne. Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria. Toxins. 2019; 11 (9):500.
Chicago/Turabian StyleAssia Mairi; Abdelaziz Touati; Alix Pantel; Karima Zenati; Alex Yahiaoui Martinez; Catherine Dunyach-Remy; Albert Sotto; Jean-Philippe Lavigne. 2019. "Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria." Toxins 11, no. 9: 500.
Introduction: The spread of carbapenemase-producing Enterobacteriaceae (CPE) represents a major public health issue. Methods allowing rapid detection of carbapenemases in developing countries are therefore urgently needed. In the current study, we developed a new in-house medium for the rapid detection of CPE isolates, especially OXA-48 producers. Methodology: A panel of 144 clinical strains previously characterized was tested on in-house Carba MTL-broth medium using four different concentrations of ertapenem (0.5 to 2 mg/L), and compared to chromID® OXA-48 and chromID® CARBA (BioMérieux) media. Results: Comparative evaluation of the Carba MTL-broth with chromID® OXA-48 and chromID® CARBA showed that chromID® OXA-48 and Carba MTL-broth had the highest sensitivity for detection of OXA-48 producers (93.9% and 100%, respectively) comparatively to chromID® CARBA (21.2%). The chromID® OXA-48 had the highest specificity (100%), as compared to the Carba MTL-broth (65.5%) and chromID® CARBA (84.4%) for the detection of OXA-48 producers. Conclusions: The in-house Carba MTL-broth developed in this study is sensitive, inexpensive, an easy-to-use phenotypic method for the detection of OXA-48-producing enterobacteria. Given the burden of pan-drug resistance, its implementation in the microbiology laboratory of developing countries could be a useful tool for rapid detection of these bacteria.
Assia Mairi; Abdelaziz Touati; Alix Pantel; Catherine Dunyach-Remy; Albert Sotto; Christophe De Champs; Jean-Philippe Lavigne. Performance of a new in-house medium Carba MTL-broth for the rapid detection of carbapenemase-producing Enterobacteriaceae. The Journal of Infection in Developing Countries 2019, 13, 591 -602.
AMA StyleAssia Mairi, Abdelaziz Touati, Alix Pantel, Catherine Dunyach-Remy, Albert Sotto, Christophe De Champs, Jean-Philippe Lavigne. Performance of a new in-house medium Carba MTL-broth for the rapid detection of carbapenemase-producing Enterobacteriaceae. The Journal of Infection in Developing Countries. 2019; 13 (7):591-602.
Chicago/Turabian StyleAssia Mairi; Abdelaziz Touati; Alix Pantel; Catherine Dunyach-Remy; Albert Sotto; Christophe De Champs; Jean-Philippe Lavigne. 2019. "Performance of a new in-house medium Carba MTL-broth for the rapid detection of carbapenemase-producing Enterobacteriaceae." The Journal of Infection in Developing Countries 13, no. 7: 591-602.
The discovery of antibiotics in 1928 and their subsequent large-scale production are considered to be one of the most important achievements in the history of medicine [1x[1]Mohr, K.I. History of antibiotics research. Curr. Top. Microbiol. Immunol. 2016; 398: 237–272https://doi.org/10.1007/82_2016_499Google ScholarSee all References1]. One of the most important discoveries after that of β-lactams was streptomycin, the first aminoglycoside discovered. The history of aminoglycosides was then marked by the successive introduction of a series of compounds (kanamycin, gentamicin, and tobramycin) for the treatment of infections due to Gram-negative bacilli [2x[2]Mingeot-Leclercq, M.P., Glupczynski, Y., and Tulkens, P.M. Aminoglycosides: activity and resistance. Antimicrob. Agents Chemother. 1999; 43: 727–737Google ScholarSee all References2].
Abdelaziz Touati. Aminoglycoside resistance mechanism inference algorithm: Implication for underlying resistance mechanisms to aminoglycosides. EBioMedicine 2019, 46, 8 -8.
AMA StyleAbdelaziz Touati. Aminoglycoside resistance mechanism inference algorithm: Implication for underlying resistance mechanisms to aminoglycosides. EBioMedicine. 2019; 46 ():8-8.
Chicago/Turabian StyleAbdelaziz Touati. 2019. "Aminoglycoside resistance mechanism inference algorithm: Implication for underlying resistance mechanisms to aminoglycosides." EBioMedicine 46, no. : 8-8.
The diffusion of carbapenemase-producing Enterobacteriaceae (CPE) represents a worldwide public health problem. This study revealed that the prevalence of OXA-48–producing enterobacteria was 4.6% (19/414) and 1.6% (7/422) in mothers and newborns, respectively, from 2 maternity units in Algeria. Previous hospital admission was an independent factor associated with an increased risk of CPE carriage in the mothers (P = .021). The low birth weight was significantly associated with this carriage in the newborns (P = .008). The screening of these bacteria is essential to prevent the dissemination of CPE.
Assia Mairi; Abdelaziz Touati; Sylia Ait Bessai; Yasmina Boutabtoub; Fazia Khelifi; Albert Sotto; Jean-Philippe Lavigne; Alix Pantel. Carbapenemase-producing Enterobacteriaceae among pregnant women and newborns in Algeria: Prevalence, molecular characterization, maternal-neonatal transmission, and risk factors for carriage. American Journal of Infection Control 2018, 47, 105 -108.
AMA StyleAssia Mairi, Abdelaziz Touati, Sylia Ait Bessai, Yasmina Boutabtoub, Fazia Khelifi, Albert Sotto, Jean-Philippe Lavigne, Alix Pantel. Carbapenemase-producing Enterobacteriaceae among pregnant women and newborns in Algeria: Prevalence, molecular characterization, maternal-neonatal transmission, and risk factors for carriage. American Journal of Infection Control. 2018; 47 (1):105-108.
Chicago/Turabian StyleAssia Mairi; Abdelaziz Touati; Sylia Ait Bessai; Yasmina Boutabtoub; Fazia Khelifi; Albert Sotto; Jean-Philippe Lavigne; Alix Pantel. 2018. "Carbapenemase-producing Enterobacteriaceae among pregnant women and newborns in Algeria: Prevalence, molecular characterization, maternal-neonatal transmission, and risk factors for carriage." American Journal of Infection Control 47, no. 1: 105-108.
The aim of this study was to investigate carbapenemase-producing Enterobacteriaceae (CPE) in companion animals. Between October 2015 and April 2016, 533 rectal swabs were obtained from healthy and diseased pets in different cities in Algeria. Samples were plated on MacConkey agar supplemented with ertapenem (0.5mg/L). Isolates were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Antimicrobial susceptibility testing was performed by the disk diffusion method. Carbapenemase, plasmid-mediated AmpC (pAmpC), extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance genes were characterised by PCR. Plasmids were extracted by the Kieser extraction method and were analysed by PCR-based replicon typing (PBRT). The epidemiological relationship between Enterobacter cloacae isolates was determined by random amplified polymorphic DNA (RAPD) analysis and multilocus sequence typing (MLST). From 533 rectal swabs, 12 Enterobacteriaceae (2.3%), including 2 Escherichia coli, 2 Klebsiella pneumoniae and 8 E. cloacae, were recovered from selection plates. The 12 strains were resistant to amoxicillin/clavulanic acid, ticarcillin, piperacillin/tazobactam and ertapenem. All isolates were susceptible to aminoglycosides, imipenem and extended-spectrum cephalosporins. PCR and sequencing identified the blaOXA-48 gene in all isolates. qnrB1 was identified in all E. cloacae isolates. Plasmid analysis showed that the blaOXA-48 gene was localised on a 7-kb untypeable plasmid. RAPD analysis demonstrated the presence of the same profile pattern in the eight E. cloacae isolates. MLST analysis showed that the E. cloacae isolates belonged to ST527. This study reports for the first time the presence of CPE in horses and pet birds in the world.
Massilia Yousfi; Abdelaziz Touati; Anaelle Muggeo; Brazane Mira; Bourouis Asma; Lucien Brasme; Thomas Guillard; Christophe De Champs. Clonal dissemination of OXA-48-producing Enterobacter cloacae isolates from companion animals in Algeria. Journal of Global Antimicrobial Resistance 2018, 12, 187 -191.
AMA StyleMassilia Yousfi, Abdelaziz Touati, Anaelle Muggeo, Brazane Mira, Bourouis Asma, Lucien Brasme, Thomas Guillard, Christophe De Champs. Clonal dissemination of OXA-48-producing Enterobacter cloacae isolates from companion animals in Algeria. Journal of Global Antimicrobial Resistance. 2018; 12 ():187-191.
Chicago/Turabian StyleMassilia Yousfi; Abdelaziz Touati; Anaelle Muggeo; Brazane Mira; Bourouis Asma; Lucien Brasme; Thomas Guillard; Christophe De Champs. 2018. "Clonal dissemination of OXA-48-producing Enterobacter cloacae isolates from companion animals in Algeria." Journal of Global Antimicrobial Resistance 12, no. : 187-191.
In this study, the presence of carbapenemase genes in Shewanella xiamenensis strains isolated from river water in Béjaïa, Algeria, was investigated. Four isolates of S. xiamenensis were isolated from water from Soummam River. The isolates were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) and sequencing of the 16S rRNA and gyrB genes. Isolates were subjected to antimicrobial susceptibility testing. Carbapenemase production was screened using phenotypic tests. PCR and sequencing were used to identify carbapenemase genes in the isolates. The genetic context of the blaOXA-48-like gene was investigated by sequencing the whole genome of strain AS58. All four S. xiamenensis strains harboured blaOXA-48-like genes. They exhibited different resistance patterns and had imipenem minimum inhibitory concentrations (MICs) of ≥0.5 mg/L. Sequencing of blaOXA-48-like genes from the S. xiamenensis isolates showed that two strains harboured blaOXA-181, one strain harboured blaOXA-199 and one strain exhibited a new variant of the blaOXA-48-like gene, named blaOXA-538. This new variant shared 98% nucleotide identity with blaOXA-162, with three amino acid changes (G201A, A213G and I219F). Conjugation assays with Escherichia coli J53 recipient were performed but no transconjugants were obtained. Analysis of the genome of AS58 Touati strain confirmed the chromosomal location of the blaOXA-538 gene. This study showed that environmental water holds a diversity of S. xiamenensis strains harbouring blaOXA-48-like genes and may play an important role in the dissemination and spread of these genes from the environment to humans.
Rima Tafoukt; Thongpan Leangapichart; Linda Hadjadj; Sofiane Bakour; Seydina M. Diene; Jean-Marc Rolain; Abdelaziz Touati. Characterisation of bla OXA-538 , a new variant of bla OXA-48 , in Shewanella xiamenensis isolated from river water in Algeria. Journal of Global Antimicrobial Resistance 2017, 13, 70 -73.
AMA StyleRima Tafoukt, Thongpan Leangapichart, Linda Hadjadj, Sofiane Bakour, Seydina M. Diene, Jean-Marc Rolain, Abdelaziz Touati. Characterisation of bla OXA-538 , a new variant of bla OXA-48 , in Shewanella xiamenensis isolated from river water in Algeria. Journal of Global Antimicrobial Resistance. 2017; 13 ():70-73.
Chicago/Turabian StyleRima Tafoukt; Thongpan Leangapichart; Linda Hadjadj; Sofiane Bakour; Seydina M. Diene; Jean-Marc Rolain; Abdelaziz Touati. 2017. "Characterisation of bla OXA-538 , a new variant of bla OXA-48 , in Shewanella xiamenensis isolated from river water in Algeria." Journal of Global Antimicrobial Resistance 13, no. : 70-73.
The aim of this study is to characterize the molecular support of antibiotic resistance in MDR K. pneumonaie recovered from inanimate surfaces between March 2012 to February 2014 in three teaching hospitals (Setif, Bejaia and Constantine) in Algeria.Forty four K. pneumoniae producing ESBL were detected and blaCTX-M-15 and blaCTX-M-3 were detected respectively in 41 and 3 isolates. These K. pneumoniae isolates producing ESBL were also resistant to gentamicin (87%), tobramicin (87%), ciprofloxacin (66%) and ofloxacin (62%). Aminoglycosides resistance genes detected were 16S rRNA methylase (armA), aminoglycoside acetyl-transferase (aac(6')-Ib), aminoglycoside nucleotidyl-transferase (aadA2) and aminoglycoside, phosphoryl-transferase (ant3"Ih-aac(6')-IId). Plasmid-mediated quinolone resistance (PMQR) genes detected were aac(6')-Ib-cr (34 isolates) and qnrB genes in (34 isolates). Multilocus sequence typing (MLST) resulted in 12 different sequence types (STs) regrouped into 5 clonal complexes (CC147, CC17, CC37, CC2 and CC23), one clonal group (CG485) and 4 singletons (ST1426, ST405, ST1308, ST873).Here, we report the detection of the ESBLs encoding gene linked with plasmid-mediated quinolone resistance (PMQR) and aminoglycosides resistance recovered from inanimate surfaces in hospital environment.
Karima Zenati; Farida Sahli; Vincent Garcia; Sofiane Bakour; Djellali Belhadi; Jean Marc Rolain; Abdelaziz Touati. Occurrence and clonal diversity of multidrug-resistant Klebsiella pneumoniae recovered from inanimate surfaces in Algerian hospital environment: First report of armA , qnrB and aac(6′)-Ib-cr genes. Journal of Global Antimicrobial Resistance 2017, 10, 148 -153.
AMA StyleKarima Zenati, Farida Sahli, Vincent Garcia, Sofiane Bakour, Djellali Belhadi, Jean Marc Rolain, Abdelaziz Touati. Occurrence and clonal diversity of multidrug-resistant Klebsiella pneumoniae recovered from inanimate surfaces in Algerian hospital environment: First report of armA , qnrB and aac(6′)-Ib-cr genes. Journal of Global Antimicrobial Resistance. 2017; 10 ():148-153.
Chicago/Turabian StyleKarima Zenati; Farida Sahli; Vincent Garcia; Sofiane Bakour; Djellali Belhadi; Jean Marc Rolain; Abdelaziz Touati. 2017. "Occurrence and clonal diversity of multidrug-resistant Klebsiella pneumoniae recovered from inanimate surfaces in Algerian hospital environment: First report of armA , qnrB and aac(6′)-Ib-cr genes." Journal of Global Antimicrobial Resistance 10, no. : 148-153.
Abdelaziz Touati; Assia Mairi; Yanis Baloul; Rym Lalaoui; Sofiane Bakour; Lilia Thighilt; Alima Gharout; Jean-Marc Rolain. First detection of Klebsiella pneumoniae producing OXA-48 in fresh vegetables from Béjaïa city, Algeria. Journal of Global Antimicrobial Resistance 2017, 9, 17 -18.
AMA StyleAbdelaziz Touati, Assia Mairi, Yanis Baloul, Rym Lalaoui, Sofiane Bakour, Lilia Thighilt, Alima Gharout, Jean-Marc Rolain. First detection of Klebsiella pneumoniae producing OXA-48 in fresh vegetables from Béjaïa city, Algeria. Journal of Global Antimicrobial Resistance. 2017; 9 ():17-18.
Chicago/Turabian StyleAbdelaziz Touati; Assia Mairi; Yanis Baloul; Rym Lalaoui; Sofiane Bakour; Lilia Thighilt; Alima Gharout; Jean-Marc Rolain. 2017. "First detection of Klebsiella pneumoniae producing OXA-48 in fresh vegetables from Béjaïa city, Algeria." Journal of Global Antimicrobial Resistance 9, no. : 17-18.
Shewanella spp. are commonly known as environmental bacteria and are most frequently isolated from aquatic areas. Currently, diseases syndromes and multidrug resistance have increasingly been reported in the genus Shewanella. Some species are associated with various infections, such as skin and soft tissue infections, as well as bacteremia. Generally, these bacteria are opportunistic and mostly affect people with an impaired immune system. This genus is also a probable vehicle and progenitor of antibiotic resistance genes. In fact, several resistance genes and mobile genetic elements have been identified in some resistant species isolated from environmental or clinical settings. These genes confer resistance to different antibiotic classes, including those used in therapies such as β-lactams and quinolones, and are generally located on the chromosome. Recently, a multidrug-resistant (MDR) plasmid harboring several drug resistance genes associated with transposons and integrons has been identified in Shewanella xiamenensis. These antibiotic resistance genes can circulate in the environment and contribute to the emergence of antibiotic resistance. This review describes different aspects of Shewanella, focusing on the infections caused by this genus, as well as their role in the propagation of antibiotic resistance via mobile genetic elements.
K. Yousfi; S. Bekal; V. Usongo; A. Touati. Current trends of human infections and antibiotic resistance of the genus Shewanella. European Journal of Clinical Microbiology & Infectious Diseases 2017, 36, 1353 -1362.
AMA StyleK. Yousfi, S. Bekal, V. Usongo, A. Touati. Current trends of human infections and antibiotic resistance of the genus Shewanella. European Journal of Clinical Microbiology & Infectious Diseases. 2017; 36 (8):1353-1362.
Chicago/Turabian StyleK. Yousfi; S. Bekal; V. Usongo; A. Touati. 2017. "Current trends of human infections and antibiotic resistance of the genus Shewanella." European Journal of Clinical Microbiology & Infectious Diseases 36, no. 8: 1353-1362.
This is the first report of CTX-M-15-producing E. coli and K. pneumoniae in wild animals from Algeria. The results show that African wildlife can act as a reservoir of the epidemic E. coli clone ST131 producing CTX-M-15, suggesting that this lineage can survive in different ecological niches and adapt to different hosts.
Taous Bachiri; Sofiane Bakour; Rabia Ladjouzi; Leangapichart Thongpan; Jean Marc Rolain; Abdelaziz Touati. High rates of CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae in wild boars and Barbary macaques in Algeria. Journal of Global Antimicrobial Resistance 2017, 8, 35 -40.
AMA StyleTaous Bachiri, Sofiane Bakour, Rabia Ladjouzi, Leangapichart Thongpan, Jean Marc Rolain, Abdelaziz Touati. High rates of CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae in wild boars and Barbary macaques in Algeria. Journal of Global Antimicrobial Resistance. 2017; 8 ():35-40.
Chicago/Turabian StyleTaous Bachiri; Sofiane Bakour; Rabia Ladjouzi; Leangapichart Thongpan; Jean Marc Rolain; Abdelaziz Touati. 2017. "High rates of CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae in wild boars and Barbary macaques in Algeria." Journal of Global Antimicrobial Resistance 8, no. : 35-40.
Quinolones are a family of synthetic broad-spectrum antimicrobial drugs. These molecules have been widely prescribed to treat various infectious diseases and have been classified into several generations based on their spectrum of activity. Quinolones inhibit bacterial DNA synthesis by interfering with the action of DNA gyrase and topoisomerase IV. Mutations in the genes encoding these targets are the most common mechanisms of high-level fluoroquinolone resistance. Moreover, three mechanisms for plasmid-mediated quinolone resistance (PMQR) have been discovered since 1998 and include Qnr proteins, the aminoglycoside acetyltransferase AAC(6′)-Ib-cr, and plasmid-mediated efflux pumps QepA and OqxAB. Plasmids with these mechanisms often encode additional antimicrobial resistance (extended spectrum beta-lactamases [ESBLs] and plasmidic AmpC [pAmpC] ß-lactamases) and can transfer multidrug resistance. The PMQR determinants are disseminated in Mediterranean countries with prevalence relatively high depending on the sources and the regions, highlighting the necessity of long-term surveillance for the future monitoring of trends in the occurrence of PMQR genes.
B. Yanat; Jose Manuel Rodriguez Martinez; A. Touati. Plasmid-mediated quinolone resistance in Enterobacteriaceae: a systematic review with a focus on Mediterranean countries. European Journal of Clinical Microbiology & Infectious Diseases 2016, 36, 421 -435.
AMA StyleB. Yanat, Jose Manuel Rodriguez Martinez, A. Touati. Plasmid-mediated quinolone resistance in Enterobacteriaceae: a systematic review with a focus on Mediterranean countries. European Journal of Clinical Microbiology & Infectious Diseases. 2016; 36 (3):421-435.
Chicago/Turabian StyleB. Yanat; Jose Manuel Rodriguez Martinez; A. Touati. 2016. "Plasmid-mediated quinolone resistance in Enterobacteriaceae: a systematic review with a focus on Mediterranean countries." European Journal of Clinical Microbiology & Infectious Diseases 36, no. 3: 421-435.
The aim of this study was to evaluate the rate of fecal carriage of Escherichia coli strains producing Extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) isolated from healthy pets (dogs and cats) in Algeria. Fecal samples from 171 healthy pets (102 dogs and 69 cats) in one veterinary practice and private owners were included. After isolates identification, antibiotic susceptibility was determined by disk diffusion procedure. ESBL were detected by combination disk tests. PCR and sequencing were used to characterize genes encoding ESBLs and PMQR. Transfer of ESBL and PMQR genes was assessed by conjugation experiments. Phylogenetic groups of E. coli were determined by PCR. Of the 171 animals, 20 carried an ESBL producing E. coli giving a prevalence of ESBL fecal carriage of 11.7%. All isolates were susceptible to carbapenems, cefoxitin, piperacillin-tazobactam, amikacin and fosfomycine. For the rest of the tested β-lactams, susceptibility rates ranged from 35% to 70% for cefepime and amoxicillin-clavulanic acid respectively. Concerning the non-beta-lactams antibiotics, the rates of susceptibility ranged between 5% to trimethoprim and 95% for chloramphenicol. The beta-lactamase genes identified in E. coli isolates were blaCTX-M-15, blaCTX-M-1, blaSHV-12 and blaTEM-1. The PMQR determinants aac(6')-Ib-cr, qnrS1 and qnrB5 genes were identified in 15 isolates. Transconjugants were obtained for two isolates. Phylogenetic analysis showed that E. coli isolates belong to commensal phylogroups of A and B1. We reported here for the first time in Algeria ESBL and PMQR-producing E. coli in healthy cats and dogs.
Massilia Yousfi; Assia Mairi; Abdelaziz Touati; Lila Hassissene; Lucien Brasme; Thomas Guillard; Christophe De Champs. Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria. Journal of Infection and Chemotherapy 2016, 22, 431 -435.
AMA StyleMassilia Yousfi, Assia Mairi, Abdelaziz Touati, Lila Hassissene, Lucien Brasme, Thomas Guillard, Christophe De Champs. Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria. Journal of Infection and Chemotherapy. 2016; 22 (7):431-435.
Chicago/Turabian StyleMassilia Yousfi; Assia Mairi; Abdelaziz Touati; Lila Hassissene; Lucien Brasme; Thomas Guillard; Christophe De Champs. 2016. "Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria." Journal of Infection and Chemotherapy 22, no. 7: 431-435.
Carbapenemase-producing isolates have been isolated from humans, environmental samples and, more recently, companion animals [1]. Among the newly emerged carbapenemases, New Delhi metallo-β-lactamase (NDM) represents the latest threat for public health [2]. Sixteen variants of NDM-type carbapenemase (NDM-1 to NDM-16) have been reported (http://www.lahey.org/Studies).
M. Yousfi; A. Mairi; Sofiane Bakour; A. Touati; L. Hassissen; L. Hadjadj; Jean-Marc Rolain. First report of NDM-5-producing Escherichia coli ST1284 isolated from dog in Bejaia, Algeria. New Microbes and New Infections 2015, 8, 17 -8.
AMA StyleM. Yousfi, A. Mairi, Sofiane Bakour, A. Touati, L. Hassissen, L. Hadjadj, Jean-Marc Rolain. First report of NDM-5-producing Escherichia coli ST1284 isolated from dog in Bejaia, Algeria. New Microbes and New Infections. 2015; 8 ():17-8.
Chicago/Turabian StyleM. Yousfi; A. Mairi; Sofiane Bakour; A. Touati; L. Hassissen; L. Hadjadj; Jean-Marc Rolain. 2015. "First report of NDM-5-producing Escherichia coli ST1284 isolated from dog in Bejaia, Algeria." New Microbes and New Infections 8, no. : 17-8.
ObjectiveTo investigate the mechanisms of quinolone resistance and the association with other resistance markers among Esherichia coli (E. coli) strains isolated from outpatient with urinary tract infection in north of Algeria.MethodsA total of 30 nalidixic acid-resistant E. coli isolates from outpatient with urinary tract infections from January 2010 to April 2011 in north of Algeria (Bejaia) were studied. Antimicrobial susceptibility was determined by disc diffusion assay, minimal inhibitory concentrations (MIC) of quinolone were determined by microdilution. Mutations in the Quinolone Resistance-Determining Region (QRDR) of gyrA and parC genes and screening for qnr (A, B and S) and bla genes were done by PCR and DNA sequencing.ResultsMost of the E. coli isolates (56.66%) were shown to carry mutations in gyrA and parC (gyrA: Ser83Leu + Asp87Asn and parC:Ser80Ile). While, 16.66% had only an alteration in gyrA: Ser83Leu. One isolate produced qnrB-like and two qnrS-like. Four isolates were CTX-M-15 producers associated with TEM-1 producing in one case. Co-expression of blaCTX-M-15 and qnrB was determined in one E. coli isolate.ConclusionsOur findings suggested the community emergence of gyrA and parC alterations and Qnr determinants that contributed to the development and spread of fluoroquinolone resistance in Algerian E. coli isolates
Yanat Betitra; Vinuesa Teresa; Viñas Miguel; Touati Abdelaziz. Determinants of quinolone resistance in Escherichia coli causing community-acquired urinary tract infection in Bejaia, Algeria. Asian Pacific Journal of Tropical Medicine 2014, 7, 462 -467.
AMA StyleYanat Betitra, Vinuesa Teresa, Viñas Miguel, Touati Abdelaziz. Determinants of quinolone resistance in Escherichia coli causing community-acquired urinary tract infection in Bejaia, Algeria. Asian Pacific Journal of Tropical Medicine. 2014; 7 (6):462-467.
Chicago/Turabian StyleYanat Betitra; Vinuesa Teresa; Viñas Miguel; Touati Abdelaziz. 2014. "Determinants of quinolone resistance in Escherichia coli causing community-acquired urinary tract infection in Bejaia, Algeria." Asian Pacific Journal of Tropical Medicine 7, no. 6: 462-467.
Two isolates of Enterobacter cloacae and 1 isolate of Acinetobacter baumannii showing a multidrug resistance phenotype including resistance to beta-lactams and fluoroquinolones were included in this study. By polymerase chain reaction amplification and sequencing, the 2 isolates of E. cloacae were found to produce QnrB and the A. baumannii isolate was found to produce QnrA. In addition, the 2 E. cloacae isolates were found to produce CTX-M-15.
Abdelaziz Touati; Lucien Brasme; Said Benallaoua; Alima Gharout; Janick Madoux; Christophe De Champs. First report of qnrB-producing Enterobacter cloacae and qnrA-producing Acinetobacter baumannii recovered from Algerian hospitals. Diagnostic Microbiology and Infectious Disease 2008, 60, 287 -290.
AMA StyleAbdelaziz Touati, Lucien Brasme, Said Benallaoua, Alima Gharout, Janick Madoux, Christophe De Champs. First report of qnrB-producing Enterobacter cloacae and qnrA-producing Acinetobacter baumannii recovered from Algerian hospitals. Diagnostic Microbiology and Infectious Disease. 2008; 60 (3):287-290.
Chicago/Turabian StyleAbdelaziz Touati; Lucien Brasme; Said Benallaoua; Alima Gharout; Janick Madoux; Christophe De Champs. 2008. "First report of qnrB-producing Enterobacter cloacae and qnrA-producing Acinetobacter baumannii recovered from Algerian hospitals." Diagnostic Microbiology and Infectious Disease 60, no. 3: 287-290.