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Neutralizing antibodies are an important part of the humoral immune response to SARS-CoV-2. It is currently unclear to what extent such antibodies are produced after non-severe disease or asymptomatic infection. We studied a cluster of SARS-CoV-2 infections among a homogeneous population of 332 predominantly male Swiss soldiers and determined the neutralizing antibody response with a serum neutralization assay using a recombinant SARS-CoV-2-GFP. All patients with non-severe COVID-19 showed a swift humoral response within two weeks after the onset of symptoms, which remained stable for the duration of the study. One month after the outbreak, titers in COVID-19 convalescents did not differ from the titers of asymptomatically infected individuals. Furthermore, symptoms of COVID-19 did not correlate with neutralizing antibody titers. Therefore, we conclude that asymptomatic infection can induce the same humoral immunity as non-severe COVID-19 in young adults.
Hulda R. Jonsdottir; Michel Bielecki; Denise Siegrist; Thomas W. Buehrer; Roland Züst; Jeremy W. Deuel. Titers of Neutralizing Antibodies against SARS-CoV-2 Are Independent of Symptoms of Non-Severe COVID-19 in Young Adults. Viruses 2021, 13, 284 .
AMA StyleHulda R. Jonsdottir, Michel Bielecki, Denise Siegrist, Thomas W. Buehrer, Roland Züst, Jeremy W. Deuel. Titers of Neutralizing Antibodies against SARS-CoV-2 Are Independent of Symptoms of Non-Severe COVID-19 in Young Adults. Viruses. 2021; 13 (2):284.
Chicago/Turabian StyleHulda R. Jonsdottir; Michel Bielecki; Denise Siegrist; Thomas W. Buehrer; Roland Züst; Jeremy W. Deuel. 2021. "Titers of Neutralizing Antibodies against SARS-CoV-2 Are Independent of Symptoms of Non-Severe COVID-19 in Young Adults." Viruses 13, no. 2: 284.
Working in accordance with biosafety level four practices is highly complex and time-consuming. Therefore, the respective laboratory protocols should be as uniform as possible, simple to perform and straightforward in readout. Here we describe the successful application of a standardized 24-well plate focus assay protocol for the titration of Zaire ebolavirus (two isolates), Marburg virus (three isolates), Lassa virus (two isolates), Crimean Congo hemorrhagic fever virus (one isolate), and tick-borne encephalitis virus (two isolates). Viral titers are determined based on a simple visual readout. The protocol exhibits high precision, with coefficients of variation for interassay variability ranging between 0.05 and 0.21 and those for intraassay variability between 0.08 and 0.23. All reagents required for the test, including primary and secondary antibodies, are commercially available, facilitating the establishment of the protocol in other laboratories.
Rahel Ackermann-Gäumann; Denise Siegrist; Roland Züst; Johanna Signer; Nicole Lenz; Olivier Engler. Standardized focus assay protocol for biosafety level four viruses. Journal of Virological Methods 2018, 264, 51 -54.
AMA StyleRahel Ackermann-Gäumann, Denise Siegrist, Roland Züst, Johanna Signer, Nicole Lenz, Olivier Engler. Standardized focus assay protocol for biosafety level four viruses. Journal of Virological Methods. 2018; 264 ():51-54.
Chicago/Turabian StyleRahel Ackermann-Gäumann; Denise Siegrist; Roland Züst; Johanna Signer; Nicole Lenz; Olivier Engler. 2018. "Standardized focus assay protocol for biosafety level four viruses." Journal of Virological Methods 264, no. : 51-54.