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Dr. Ioanna Ntaikou
Institute of Chemical Engineering Sciences, Foundation for Research and Technology

Basic Info


Research Keywords & Expertise

0 Risk Assessment
0 Bioethanol production
0 Environmental biotechnology and biochemical engineering
0 Biohydrogen production
0 Polyhydroxyalkanoates (PHA)

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Bioethanol production
Biohydrogen production
Lignocellulosic biomass pretreatment and biorefinery

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Short Biography

Dr. Ioanna Ntaikou is a biologist, holding a MSc on Energy & Environment and a PhD on Chemical Engineering. Her research expertise is on Environmental and Biochemical Engineering with emphasis on in applied microbiology processes for wastewater treatment and valorisation, environmental risk assessment via biomarkers and modelling of biochemical processes. Since 2009 she is an active member of the academia taking over the pre-graduate courses General Microbiology, Fermentation/Biotechnology, Industrial Fermentations (2009-2016, Department of Food Technology, ATEI of Peloponnese), Applied Microbiology (2016-2017, Department of Biology, University of Patras) and Biology, Food Microbiology (2019-2021, Department of Food Science &Technology, University of Patras). She is author/co-author of 36 publications in international peer-reviewed journals (h-index = 17, citations ~ 1100), more than 70 publications in Conference Proceedings and 6 book chapters. She is Editor of the International Journal of Biobased Plastics (Taylor & Francis) and member of the Editorial Board of Sustainability (MDPI). She has participated in 26 National and International Research programs as associate researcher or Project Manager.

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Journal article
Published: 12 July 2021 in Toxics
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Considering that electronic wastes (e-wastes) have been recently recognized as a potent environmental and human threat, the present study aimed to assess the potential risk of personal computer motherboards (PCMBs) leaching into aquatic media, following a real-life scenario. Specifically, PCMBs were submerged for 30 days in both distilled water (DW) and artificial seawater (ASW). Afterwards, PCMBs leachates were chemically characterized (i.e., total organic carbon, ions, and trace elements) and finally used (a) for culturing freshwater (Chlorococcum sp. and Scenedesmus rubescens) and saltwater (Dunaliella tertiolecta and Tisochrysis lutea) microalgae for 10 days (240 h), (b) as the exposure medium for mussel Mytilus galloprovincialis (96 h exposure), and (c) for performing the Cytokinesis Block Micronucleus (CBMN) assay in human lymphocytes cultures. According to the results, PCMBs could mediate both fresh- and marine algae growth rates over time, thus enhancing the cytotoxic, oxidative, and genotoxic effects in the hemocytes of mussels (in terms of lysosomal membrane impairment, lipid peroxidation, and NO content and micronuclei formation, respectively), as well as human lymphocytes (in terms of MN formation and CBPI values, respectively). The current findings clearly revealed that PCMBs leaching into the aquatic media could pose detrimental effects on both aquatic organisms and human cells.

ACS Style

Georgios Kalamaras; Maria Kloukinioti; Maria Antonopoulou; Ioanna Ntaikou; Dimitris Vlastos; Antonios Eleftherianos; Stefanos Dailianis. The Potential Risk of Electronic Waste Disposal into Aquatic Media: The Case of Personal Computer Motherboards. Toxics 2021, 9, 166 .

AMA Style

Georgios Kalamaras, Maria Kloukinioti, Maria Antonopoulou, Ioanna Ntaikou, Dimitris Vlastos, Antonios Eleftherianos, Stefanos Dailianis. The Potential Risk of Electronic Waste Disposal into Aquatic Media: The Case of Personal Computer Motherboards. Toxics. 2021; 9 (7):166.

Chicago/Turabian Style

Georgios Kalamaras; Maria Kloukinioti; Maria Antonopoulou; Ioanna Ntaikou; Dimitris Vlastos; Antonios Eleftherianos; Stefanos Dailianis. 2021. "The Potential Risk of Electronic Waste Disposal into Aquatic Media: The Case of Personal Computer Motherboards." Toxics 9, no. 7: 166.

Journal article
Published: 09 February 2021 in Sustainability
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The present study aims to assess the impact of the type of yeast consortium used during bioethanol production from starchy biowastes and to determine the optimal fermentation conditions for enhanced bioethanol production. Three different yeast strains, Saccharomyces cerevisiae, Pichia barkeri, and Candida intermedia were used in mono- and co-cultures with pretreated waste-rice as substrate. The optimization of fermentation conditions i.e., fermentation time, temperature, pH, and inoculum size, was investigated in small-scale batch cultures and subsequently, the optimal conditions were applied for scaling-up and validation of the process in a 7-L fermenter. It was shown that co-culturing of yeasts either in couples or triples significantly enhanced the fermentation efficiency of the process, with ethanol yield reaching 167.80 ± 0.49 g/kg of biowaste during experiments in the fermenter.

ACS Style

Mohamed Hashem; Saad Alamri; Tahani Asseri; Yasser Moustafa; Gerasimos Lyberatos; Ioanna Ntaikou. On the Optimization of Fermentation Conditions for Enhanced Bioethanol Yields from Starchy Biowaste Via Yeast Co-Cultures. Sustainability 2021, 13, 1890 .

AMA Style

Mohamed Hashem, Saad Alamri, Tahani Asseri, Yasser Moustafa, Gerasimos Lyberatos, Ioanna Ntaikou. On the Optimization of Fermentation Conditions for Enhanced Bioethanol Yields from Starchy Biowaste Via Yeast Co-Cultures. Sustainability. 2021; 13 (4):1890.

Chicago/Turabian Style

Mohamed Hashem; Saad Alamri; Tahani Asseri; Yasser Moustafa; Gerasimos Lyberatos; Ioanna Ntaikou. 2021. "On the Optimization of Fermentation Conditions for Enhanced Bioethanol Yields from Starchy Biowaste Via Yeast Co-Cultures." Sustainability 13, no. 4: 1890.

Journal article
Published: 30 December 2020 in Sustainability
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In the current study, a domestic food waste containing more than 50% of carbohydrates was assessed as feedstock to produce second-generation bioethanol. Aiming to the maximum exploitation of the carbohydrate fraction of the waste, its hydrolysis via cellulolytic and amylolytic enzymatic blends was investigated and the saccharification efficiency was assessed in each case. Fermentation experiments were performed using the non-conventional yeast Pichia anomala (Wickerhamomyces anomalus) under both separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) modes to evaluate the conversion efficiencies and ethanol yields for different enzymatic loadings. It was shown that the fermentation efficiency of the yeast was not affected by the fermentation mode and was high for all handlings, reaching 83%, whereas the enzymatic blend containing the highest amount of both cellulolytic and amylolytic enzymes led to almost complete liquefaction of the waste, resulting also in ethanol yields reaching 141.06 ± 6.81 g ethanol/kg waste (0.40 ± 0.03 g ethanol/g consumed carbohydrates). In the sequel, a scale-up fermentation experiment was performed with the highest loading of enzymes in SHF mode, from which the maximum specific growth rate, μmax, and the biomass yield, Yx/s, of the yeast from the hydrolyzed waste were estimated. The ethanol yields that were achieved were similar to those of the respective small scale experiments reaching 138.67 ± 5.69 g ethanol/kg waste (0.40 ± 0.01 g ethanol/g consumed carbohydrates).

ACS Style

Ioanna Ntaikou; Georgia Antonopoulou; Gerasimos Lyberatos. Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst. Sustainability 2020, 13, 259 .

AMA Style

Ioanna Ntaikou, Georgia Antonopoulou, Gerasimos Lyberatos. Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst. Sustainability. 2020; 13 (1):259.

Chicago/Turabian Style

Ioanna Ntaikou; Georgia Antonopoulou; Gerasimos Lyberatos. 2020. "Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst." Sustainability 13, no. 1: 259.

Book chapter
Published: 02 October 2020 in Sustainable Fuel Technologies Handbook
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ACS Style

Ioanna Ntaikou. Microbial production of hydrogen. Sustainable Fuel Technologies Handbook 2020, 315 -337.

AMA Style

Ioanna Ntaikou. Microbial production of hydrogen. Sustainable Fuel Technologies Handbook. 2020; ():315-337.

Chicago/Turabian Style

Ioanna Ntaikou. 2020. "Microbial production of hydrogen." Sustainable Fuel Technologies Handbook , no. : 315-337.

Waste and biomass management and valorization
Published: 17 June 2020 in Environmental Science and Pollution Research
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The present study investigated the effect of thermo-chemical pretreatment on the enhancement of enzymatic digestibility of olive mill stones (OMS), as well as its possible valorisation via bioconversion of the generated free sugars to alcohols. Specifically, the influence of parameters such as reaction time, temperature, type and concentration of dilute acids and/or bases, was assessed during the thermo-chemical pretreatment. The hydrolysates and the solids remaining after pretreatment, as well as the whole pretreated slurries, were further evaluated as potential substrates for the simultaneous production of ethanol and xylitol via fermentation with the yeast Pachysolen tannophilus. The digestibility and overall saccharification of OMS were considerably enhanced in all cases, with the maximum enzymatic digestibility observed for dilute sodium hydroxide (almost 4-fold) which also yielded the highest total saccharification yield (91% of the total OMS carbohydrates). Ethanol and xylitol yields from the untreated OMS were 28 g/kg OMS and 25 g/kg OMS, respectively, and were both significantly enhanced by pretreatment. The highest ethanol yield was 79 g/kg OMS and was achieved by the alkali pretreatment and separate fermentation of hydrolysates and solids, whereas the highest xylitol yield was 49 g/kg OMS and was obtained by pretreatment with sulphuric acid and separate fermentation of hydrolysates and solids.

ACS Style

Ioanna Ntaikou; Sviatlana Siankevich; Gerasimos Lyberatos. Effect of thermo-chemical pretreatment on the saccharification and enzymatic digestibility of olive mill stones and their bioconversion towards alcohols. Environmental Science and Pollution Research 2020, 28, 24570 -24579.

AMA Style

Ioanna Ntaikou, Sviatlana Siankevich, Gerasimos Lyberatos. Effect of thermo-chemical pretreatment on the saccharification and enzymatic digestibility of olive mill stones and their bioconversion towards alcohols. Environmental Science and Pollution Research. 2020; 28 (19):24570-24579.

Chicago/Turabian Style

Ioanna Ntaikou; Sviatlana Siankevich; Gerasimos Lyberatos. 2020. "Effect of thermo-chemical pretreatment on the saccharification and enzymatic digestibility of olive mill stones and their bioconversion towards alcohols." Environmental Science and Pollution Research 28, no. 19: 24570-24579.

Journal article
Published: 07 May 2020 in Science of The Total Environment
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In the present study the bioconversion of dried household food waste (FORBI) to energy carriers was investigated aiming to its sustainable management and valorization. FORBI was either directly fermented towards ethanol and hydrogen or was previously subjected to extraction with water resulting to a liquid fraction (extract) rich in sugars and a solid residue, which were then fermented separately. Subsequently, the effluents were assessed as substrates for methane production via anaerobic digestion (AD). Mono-cultures and co-cultures of C5 and C6 yeasts were used for the alcoholic fermentation whereas for the production of hydrogen, mixed acidogenic consortia were used. Taking into account the optimum yields of biofuels, the amount of recoverable energy was estimated based for each different approach. The maximum ethanol yield was 0.16 g ethanol per kg of FORBI and it was achieved for separate fermentation of liquid and solid fractions of the waste. The highest hydrogen yield that was observed was 210.44 L ± 4.02 H2/kg TS FORBI for 1% solids loading and supplementation with cellulolytic enzymes. Direct AD of either the whole FORBI or its individual fractions led to lower overall energy recovery, compared to that obtained when fermentation and subsequent AD were applied. The recoverable energy was estimated for the different exploitation approaches of the waste. The maximum achieved recoverable energy was 21.49 ± 0.57 MJ/kg.

ACS Style

Georgia Antonopoulou; Maria Alexandropoulou; Ioanna Ntaikou; Gerasimos Lyberatos. From waste to fuel: Energy recovery from household food waste via its bioconversion to energy carriers based on microbiological processes. Science of The Total Environment 2020, 732, 139230 .

AMA Style

Georgia Antonopoulou, Maria Alexandropoulou, Ioanna Ntaikou, Gerasimos Lyberatos. From waste to fuel: Energy recovery from household food waste via its bioconversion to energy carriers based on microbiological processes. Science of The Total Environment. 2020; 732 ():139230.

Chicago/Turabian Style

Georgia Antonopoulou; Maria Alexandropoulou; Ioanna Ntaikou; Gerasimos Lyberatos. 2020. "From waste to fuel: Energy recovery from household food waste via its bioconversion to energy carriers based on microbiological processes." Science of The Total Environment 732, no. : 139230.

Journal article
Published: 23 March 2020 in Renewable Energy
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In the present study, electrocoagulation (EC), alone or in combination with precipitation or centrifugation, was assessed as a potential pretreatment method for enhanced production of biofuels from 3-phase decanter olive mill wastewater (OMW). Experiments were performed using undiluted and diluted OMW in an EC reactor, operating with a current of 2 A and either Al or Fe electrode materials. Subsequently, diluted OMW was subjected to EC followed by precipitation or centrifugation. The effect of current density (0.05, 0.5 or 1 A) on the combined EC – centrifugation process was assessed and a high reduction of organics was observed for the higher current applied. The different fractions of the pretreated effluents were used as substrates for methane, hydrogen as well as for bioethanol production and the maximum energy recovery was determined in each case. It was shown that, EC at a current of 2 A or further combination with physical methods led to low biofuels yields. However, reduction of the current during EC to 0.05 A or 0.5 A, led to high methane and ethanol yields, with the best scenario being the production of methane yielding an energy equivalent of 1902 kJ/L OMW.

ACS Style

Ioanna Ntaikou; G. Antonopoulou; D. Vayenas; G. Lyberatos. Assessment of electrocoagulation as a pretreatment method of olive mill wastewater towards alternative processes for biofuels production. Renewable Energy 2020, 154, 1252 -1262.

AMA Style

Ioanna Ntaikou, G. Antonopoulou, D. Vayenas, G. Lyberatos. Assessment of electrocoagulation as a pretreatment method of olive mill wastewater towards alternative processes for biofuels production. Renewable Energy. 2020; 154 ():1252-1262.

Chicago/Turabian Style

Ioanna Ntaikou; G. Antonopoulou; D. Vayenas; G. Lyberatos. 2020. "Assessment of electrocoagulation as a pretreatment method of olive mill wastewater towards alternative processes for biofuels production." Renewable Energy 154, no. : 1252-1262.

Journal article
Published: 05 March 2020 in Renewable Energy
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In the present study the non-conventional yeast Wickerhamomyces anomalus X19, was evaluated as biocatalyst for direct, low cost bioethanol production, using natural date palm sap (DPS) as substrate. Initial experiments were conducted with synthetic media in order to characterize the strain in terms of its nitrogen requirements, the effect of initial substrate concentration on the ethanol yields and the main kinetic constants during alcoholic fermentation. Subsequently, the effect of aerobic or anaerobic conditions was assessed with DPS as substrate, in batch mode and the possible improvement of ethanol yields was further investigated via fed batch experiments. It was shown that W. anomalus X19 was not inhibited by low pH (<3) and high initial sugar concentrations (up to 160 g/L), leading in all cases to ethanol yields that exceeded 80% of the theoretically estimated values. The highest ethanol concentration from DPS fermentation was achieved in fed batch experiments, reaching 73.11 g/L and the ethanol yield was 0.46 g/g sugars, which corresponded to more than 90% of the theoretical maximum.

ACS Style

Imen Ben Atitallah; Ioanna Ntaikou; Georgia Antonopoulou; Maria Alexandropoulou; Michael Brysch-Herzberg; Moncef Nasri; Gerasimos Lyberatos; Tahar Mechichi. Evaluation of the non-conventional yeast strain Wickerhamomyces anomalus (Pichia anomala) X19 for enhanced bioethanol production using date palm sap as renewable feedstock. Renewable Energy 2020, 154, 71 -81.

AMA Style

Imen Ben Atitallah, Ioanna Ntaikou, Georgia Antonopoulou, Maria Alexandropoulou, Michael Brysch-Herzberg, Moncef Nasri, Gerasimos Lyberatos, Tahar Mechichi. Evaluation of the non-conventional yeast strain Wickerhamomyces anomalus (Pichia anomala) X19 for enhanced bioethanol production using date palm sap as renewable feedstock. Renewable Energy. 2020; 154 ():71-81.

Chicago/Turabian Style

Imen Ben Atitallah; Ioanna Ntaikou; Georgia Antonopoulou; Maria Alexandropoulou; Michael Brysch-Herzberg; Moncef Nasri; Gerasimos Lyberatos; Tahar Mechichi. 2020. "Evaluation of the non-conventional yeast strain Wickerhamomyces anomalus (Pichia anomala) X19 for enhanced bioethanol production using date palm sap as renewable feedstock." Renewable Energy 154, no. : 71-81.

Original article
Published: 28 February 2020 in Biomass Conversion and Biorefinery
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In the present study, the valorization of cheese whey (CW) as an electron donor in an air-cathode single-chamber microbial fuel cell (MFC) was studied. Filter-sterilized raw and pretreated-acidified diluted CW (after 48 h of fermentation at mesophilic temperature) were used as substrates, in order to investigate the effect of the two different handlings on the MFC performance. The pretreatment-acidification experiments were performed under different operational conditions (initial dilutions giving chemical oxygen demand (COD) concentrations of 2 and 4 g/L as well as initial pH adjusted to 6.7 and without pH adjustment) in order to obtain maximum acidification efficiency and energy recovery, in the form of hydrogen. The effect of organic load on the efficiency of the MFC was studied, aiming at exploring the possibility of achieving a successful operation at the highest possible initial concentration of CW (smallest dilution). The experimental results showed that CW is a suitable and promising substrate for electricity production using MFC, with a maximum power density of 3.26 W/m3 (0.33 MJ/kg COD) for filter-sterilized CW diluted to an initial concentration of 0.8 g COD/L. Combining MFC technology with the pretreatment/acidification process, during which hydrogen is also produced, a total energy of 2.37 MJ/kg COD could be recovered. Graphical abstract

ACS Style

G. Antonopoulou; Ioanna Ntaikou; S. Bebelis; G. Lyberatos. On the evaluation of filtered and pretreated cheese whey as an electron donor in a single chamber microbial fuel cell. Biomass Conversion and Biorefinery 2020, 11, 633 -643.

AMA Style

G. Antonopoulou, Ioanna Ntaikou, S. Bebelis, G. Lyberatos. On the evaluation of filtered and pretreated cheese whey as an electron donor in a single chamber microbial fuel cell. Biomass Conversion and Biorefinery. 2020; 11 (2):633-643.

Chicago/Turabian Style

G. Antonopoulou; Ioanna Ntaikou; S. Bebelis; G. Lyberatos. 2020. "On the evaluation of filtered and pretreated cheese whey as an electron donor in a single chamber microbial fuel cell." Biomass Conversion and Biorefinery 11, no. 2: 633-643.

Original research article
Published: 24 October 2019 in Frontiers in Sustainable Food Systems
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In the present study, the effect of thermo-chemical pretreatment and enzymatic hydrolysis on the saccharification and the further bioconversion of two different types of lignocellulosic agro-industrial residues to bioethanol and methane, was investigated. The residues used were the sunflower straw (SS) and the cracked olive stones (OS) remaining after the olive oil production process. Biomasses were subjected to simultaneous chemical and thermal pretreatment using either dilute H2SO4 or dilute NaOH aqueous solutions of concentrations 0.5, 1, and 1.5% (w/v), at 121°C for 60 min. The saccharification of the complex carbohydrates due to pretreatment was determined in terms of the sugars released, and the effect of each pretreatment was evaluated statistically. The effect of the enzymatic hydrolysis of the biomasses using commercial cellulolytic enzymes on saccharification was also assessed and statistically evaluated in terms of different enzymatic loadings. Subsequently, the pre-treated wastes were used as a substrate for methane and ethanol production using mixed methanogenic consortia and the yeast Pachysolen tannophilus, respectively.

ACS Style

Georgia Antonopoulou; Anastasios Kampranis; Ioanna Ntaikou; Gerasimos Lyberatos. Enhancement of Liquid and Gaseous Biofuels Production From Agro-Industrial Residues After Thermochemical and Enzymatic Pretreatment. Frontiers in Sustainable Food Systems 2019, 3, 1 .

AMA Style

Georgia Antonopoulou, Anastasios Kampranis, Ioanna Ntaikou, Gerasimos Lyberatos. Enhancement of Liquid and Gaseous Biofuels Production From Agro-Industrial Residues After Thermochemical and Enzymatic Pretreatment. Frontiers in Sustainable Food Systems. 2019; 3 ():1.

Chicago/Turabian Style

Georgia Antonopoulou; Anastasios Kampranis; Ioanna Ntaikou; Gerasimos Lyberatos. 2019. "Enhancement of Liquid and Gaseous Biofuels Production From Agro-Industrial Residues After Thermochemical and Enzymatic Pretreatment." Frontiers in Sustainable Food Systems 3, no. : 1.

Journal article
Published: 15 October 2019 in Beverages
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Background: The aim of the present study was to assess the microecosystem composition of three different fruit kefir grains used for the fermentation of apple juice (NAJ), cherry juice (SCN), and a solution of sugary water, enriched with plums (BSS). Methods: Yeast and bacterial populations were enumerated using classical microbiological techniques, clustered by RAPD-PCR genotyping, and identified by sequencing of the D1/D2 region of 26S-rRNA gene and the V1-V3 region of 16S-rRNA gene, respectively. The caseinolytic/lipolytic activities and the production of antimicrobial compounds were assessed by well diffusion assays. The proteolytic and lipolytic capacity were further assessed by SDS-PAGE and titrimetric assay, respectively. Results: Yeast and bacterial populations were enumerated at 6.28, 6.58, and 6.41 log CFU/g and at 4.32, 4.85, and 4.34 log CFU/g, regarding BSS, NAJ, and SCN, respectively. Saccharomyces cerevisiae dominated all three sugary kefir grains; Kluyveromyces marxianus formed a secondary microbiota in BSS and NAJ. Bacillus amyloliquefaciens dominated NAJ and SCN; Lactobacillus rhamnosus dominated BSS. Four bacteria and nine yeast isolates exhibited proteolytic activity. Forty bacteria and 45 yeast isolates possessed lipolytic activity. No antibacterial activity was detected. Conclusions: Prevalence of yeast over bacterial populations was evident in all samples assessed. Several bacterial and yeast strains exhibited proteolytic and lipolytic activities, making them suitable candidates for inclusion in starter cultures for milk and sugary kefir fermentation.

ACS Style

Maria K. Syrokou; Marina Papadelli; Ioanna Ntaikou; Spiros Paramithiotis; Eleftherios H. Drosinos. Sugary Kefir: Microbial Identification and Biotechnological Properties. Beverages 2019, 5, 61 .

AMA Style

Maria K. Syrokou, Marina Papadelli, Ioanna Ntaikou, Spiros Paramithiotis, Eleftherios H. Drosinos. Sugary Kefir: Microbial Identification and Biotechnological Properties. Beverages. 2019; 5 (4):61.

Chicago/Turabian Style

Maria K. Syrokou; Marina Papadelli; Ioanna Ntaikou; Spiros Paramithiotis; Eleftherios H. Drosinos. 2019. "Sugary Kefir: Microbial Identification and Biotechnological Properties." Beverages 5, no. 4: 61.

Journal article
Published: 07 June 2019 in Bioresource Technology
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The present study focuses on the exploration of the potential use of potato peels waste (PPW) as feedstock for bioethanol production, using a newly isolated yeast strain, Wickerhamomyces anomalus, via different saccharification and fermentation schemes. The saccharification of PPW was performed via thermal and chemical (acid, alkali) pretreatment, as well as via enzymatic hydrolysis through the use of commercial enzymes (cellulase and amylase) or enzymes produced at lab scale (alpha-amylase from Bacillus sp. Gb67), either separately or in mixtures. The results indicated that the enzymatic treatment by commercial enzymes led to a higher saccharification efficiency (72.38 %) and ethanol yield (0.49 g/g consumed sugars) corresponding to 96 % of the maximum theoretical. In addition, acid pretreatment was found to be beneficial for the process, leading also to high hydrolysis and ethanol yields, indicating that PPW is a very promising feedstock for bio-ethanol production by W. anomalus under different process schemes.

ACS Style

Imen Ben Atitallah; Georgia Antonopoulou; Ioanna Ntaikou; Maria Alexandropoulou; Moncef Nasri; Tahar Mechichi; Gerasimos Lyberatos. On the evaluation of different saccharification schemes for enhanced bioethanol production from potato peels waste via a newly isolated yeast strain of Wickerhamomyces anomalus. Bioresource Technology 2019, 289, 121614 .

AMA Style

Imen Ben Atitallah, Georgia Antonopoulou, Ioanna Ntaikou, Maria Alexandropoulou, Moncef Nasri, Tahar Mechichi, Gerasimos Lyberatos. On the evaluation of different saccharification schemes for enhanced bioethanol production from potato peels waste via a newly isolated yeast strain of Wickerhamomyces anomalus. Bioresource Technology. 2019; 289 ():121614.

Chicago/Turabian Style

Imen Ben Atitallah; Georgia Antonopoulou; Ioanna Ntaikou; Maria Alexandropoulou; Moncef Nasri; Tahar Mechichi; Gerasimos Lyberatos. 2019. "On the evaluation of different saccharification schemes for enhanced bioethanol production from potato peels waste via a newly isolated yeast strain of Wickerhamomyces anomalus." Bioresource Technology 289, no. : 121614.

Journal article
Published: 22 March 2019 in Applied Energy
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The valorization of typical household food waste (HFW) produced at municipality level was studied for the production of electricity in a microbial fuel cell (MFC) from its extract, and methane, through anaerobic digestion of the solid extraction residue. HFW, after heat drying and shredding, was subjected to extraction using warm water, which resulted in a liquid fraction (extract) and a solid residue. The rich in soluble chemical oxygen demand extract was used for electricity production in a four air– cathodes single chamber MFC, operating under different organic loading rates, while the solid residue from the extraction process was used as substrate for methane production in biochemical methane potential experiments. On the basis of the energy outputs estimated for the optimum operational conditions of both aforementioned processes, it can be concluded that the exploitation of dried HFW is quite appealing as it leads to promising energy recovery.

ACS Style

G. Antonopoulou; I. Ntaikou; Carlo Pastore; L. di Bitonto; S. Bebelis; G. Lyberatos. An overall perspective for the energetic valorization of household food waste using microbial fuel cell technology of its extract, coupled with anaerobic digestion of the solid residue. Applied Energy 2019, 242, 1064 -1073.

AMA Style

G. Antonopoulou, I. Ntaikou, Carlo Pastore, L. di Bitonto, S. Bebelis, G. Lyberatos. An overall perspective for the energetic valorization of household food waste using microbial fuel cell technology of its extract, coupled with anaerobic digestion of the solid residue. Applied Energy. 2019; 242 ():1064-1073.

Chicago/Turabian Style

G. Antonopoulou; I. Ntaikou; Carlo Pastore; L. di Bitonto; S. Bebelis; G. Lyberatos. 2019. "An overall perspective for the energetic valorization of household food waste using microbial fuel cell technology of its extract, coupled with anaerobic digestion of the solid residue." Applied Energy 242, no. : 1064-1073.

Original research article
Published: 02 January 2019 in International Journal of Biobased Plastics
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In the present study, a soil-derived mixed microbial consortium was developed for polyhydroxyalkanoates (PHAs) production via nutrient limitation. The enhanced consortium was then cultured continuously in sequential batch and the effect of different nitrogen to carbon ratios (N:C) on the yields and properties of produced PHAs and on the changes of the microbial population were investigated. In all cases, the produced polymers were identified as blends or co-polymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units, with 3HB being the dominant monomer. The degradation profile and the transition temperatures of the produced PHAs were further assessed and compared, as well as the molecular weights which ranged from 77.104 Da to 180.104 Da. In order to further investigate the effect of culturing conditions on the development and alteration of the microbial consortium, the Nile blue live staining was applied in the mixed cultures at the end of each operational period. Various fluorescent single colonies were selected based on differences in their morphology and were identified. Finally, the alterations in microbial diversity and community composition assessed via the RISA profiling method (rRNA Intergenic Spacer Analyses), resulted in the identification of a number of dominant organisms.

ACS Style

Ioanna Ntaikou; Ioannis Koumelis; Maria Kamilari; Zacharoula Iatridi; Constantinos Tsitsilianis; Gerasimos Lyberatos. Effect of nitrogen limitation on polyhydroxyalkanoates production efficiency, properties and microbial dynamics using a soil-derived mixed continuous culture. International Journal of Biobased Plastics 2019, 1, 31 -47.

AMA Style

Ioanna Ntaikou, Ioannis Koumelis, Maria Kamilari, Zacharoula Iatridi, Constantinos Tsitsilianis, Gerasimos Lyberatos. Effect of nitrogen limitation on polyhydroxyalkanoates production efficiency, properties and microbial dynamics using a soil-derived mixed continuous culture. International Journal of Biobased Plastics. 2019; 1 (1):31-47.

Chicago/Turabian Style

Ioanna Ntaikou; Ioannis Koumelis; Maria Kamilari; Zacharoula Iatridi; Constantinos Tsitsilianis; Gerasimos Lyberatos. 2019. "Effect of nitrogen limitation on polyhydroxyalkanoates production efficiency, properties and microbial dynamics using a soil-derived mixed continuous culture." International Journal of Biobased Plastics 1, no. 1: 31-47.

Journal article
Published: 01 October 2018 in Bioresource Technology
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A combined Lewis-Brønsted acid ethanolysis of sugars was thoroughly investigated with the aim of producing ethyl levulinate (EL) in a single step. Ethanolysis carried out at 453 K for 4 h using H2SO4 (1 wt %) and AlCl3∙6H2O (30 mol % with respect to sugars) produced a yield of 60 mol % of EL respect to glucose and starch. Such optimised conditions were positively applied directly on different food waste, preliminarly characterised and found to be mainly composed by simple (10-15%) and relatively complex sugars (20-60%), besides proteins (6-10%) and lipids (4-10%), even in their wet form. The catalytic system resulted robust enough to the point that the copresence of proteins, lignin, lipids and mineral salts not only did not negatively affect the overall reactivity, but resulted efficiently converted into soluble species, and specifically, into other liquid biofuels of different nature.

ACS Style

Luigi di Bitonto; Georgia Antonopoulou; Camilla Braguglia; Claudia Campanale; Agata Gallipoli; Gerasimos Lyberatos; Ioanna Ntaikou; Carlo Pastore. Lewis-Brønsted acid catalysed ethanolysis of the organic fraction of municipal solid waste for efficient production of biofuels. Bioresource Technology 2018, 266, 297 -305.

AMA Style

Luigi di Bitonto, Georgia Antonopoulou, Camilla Braguglia, Claudia Campanale, Agata Gallipoli, Gerasimos Lyberatos, Ioanna Ntaikou, Carlo Pastore. Lewis-Brønsted acid catalysed ethanolysis of the organic fraction of municipal solid waste for efficient production of biofuels. Bioresource Technology. 2018; 266 ():297-305.

Chicago/Turabian Style

Luigi di Bitonto; Georgia Antonopoulou; Camilla Braguglia; Claudia Campanale; Agata Gallipoli; Gerasimos Lyberatos; Ioanna Ntaikou; Carlo Pastore. 2018. "Lewis-Brønsted acid catalysed ethanolysis of the organic fraction of municipal solid waste for efficient production of biofuels." Bioresource Technology 266, no. : 297-305.

Journal article
Published: 01 June 2018 in International Journal of Biological Macromolecules
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The accumulation efficiency and the properties of polyhydroxyalkanoates (PHAs) produced from acidified waste glycerol (AWG) and its derivatives via an enriched microbial consortium derived from soil, were investigated in this study. AWG consisted mainly from short chain fatty acids, 1,3 propanediol and residual glycerol, which were also evaluated individually as substrates. Accumulation capacity and yields were estimated after solvent extraction and purification, and PHAs were further analyzed in terms of their chemical structure, thermal properties, molecular masses and mechanical properties. The lowest accumulation capacity was noticed for non-acidified waste glycerol as carbon source which led to the generation of P(3HB), whereas for the other carbon sources co-polymers of 3HB with 3HV or 3HHx were produced. Average molecular mass weights were quite high in all cases reaching ~1.8 × 106 Da. The thermal properties and the mechanical behavior of PHAs was shown to be highly affected by their monomeric composition, whereas it was also concluded that DSC and DMA results were in good agreement.

ACS Style

Ioanna Ntaikou; Ioannis Koumelis; Constantinos Tsitsilianis; John Parthenios; Gerasimos Lyberatos. Comparison of yields and properties of microbial polyhydroxyalkanoates generated from waste glycerol based substrates. International Journal of Biological Macromolecules 2018, 112, 273 -283.

AMA Style

Ioanna Ntaikou, Ioannis Koumelis, Constantinos Tsitsilianis, John Parthenios, Gerasimos Lyberatos. Comparison of yields and properties of microbial polyhydroxyalkanoates generated from waste glycerol based substrates. International Journal of Biological Macromolecules. 2018; 112 ():273-283.

Chicago/Turabian Style

Ioanna Ntaikou; Ioannis Koumelis; Constantinos Tsitsilianis; John Parthenios; Gerasimos Lyberatos. 2018. "Comparison of yields and properties of microbial polyhydroxyalkanoates generated from waste glycerol based substrates." International Journal of Biological Macromolecules 112, no. : 273-283.

Journal article
Published: 30 April 2018 in Bioresource Technology
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The biotransformation of the pre-dried and shredded organic fraction of kitchen waste to ethanol was investigated, via co-cultures of the yeasts Saccharomyces cerevisiae and Pichia stipitis (Scheffersomyces stipitis). Preliminary experiments with synthetic media were performed, in order to investigate the effect of different operational parameters on the ethanol production efficiency of the co-culture. The control of the pH and the supplementation with organic nitrogen were shown to be key factors for the optimization of the process. Subsequently, the ethanol production efficiency from the waste was assessed via simultaneous saccharification and fermentation experiments. Different loadings of cellulolytic enzymes and mixtures of cellulolytic with amylolytic enzymatic blends were tested in order to enhance the substrate conversion efficiency. It was further shown that for solids loading up to 40% waste on dry mass basis, corresponding to 170 g.L−1 initial concentration of carbohydrates, no substrate inhibition occurred, and ethanol concentration up to 45 g.L−1 was achieved.

ACS Style

Ioanna Ntaikou; Nikolaos Menis; Maria Alexandropoulou; Georgia Antonopoulou; Gerasimos Lyberatos. Valorization of kitchen biowaste for ethanol production via simultaneous saccharification and fermentation using co-cultures of the yeasts Saccharomyces cerevisiae and Pichia stipitis. Bioresource Technology 2018, 263, 75 -83.

AMA Style

Ioanna Ntaikou, Nikolaos Menis, Maria Alexandropoulou, Georgia Antonopoulou, Gerasimos Lyberatos. Valorization of kitchen biowaste for ethanol production via simultaneous saccharification and fermentation using co-cultures of the yeasts Saccharomyces cerevisiae and Pichia stipitis. Bioresource Technology. 2018; 263 ():75-83.

Chicago/Turabian Style

Ioanna Ntaikou; Nikolaos Menis; Maria Alexandropoulou; Georgia Antonopoulou; Gerasimos Lyberatos. 2018. "Valorization of kitchen biowaste for ethanol production via simultaneous saccharification and fermentation using co-cultures of the yeasts Saccharomyces cerevisiae and Pichia stipitis." Bioresource Technology 263, no. : 75-83.

Journal article
Published: 01 December 2017 in Journal of Environmental Management
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In this study fungal pretreatment of willow sawdust (WSD) via the white rot fungi Leiotrametes menziesii and Abortiporus biennis was studied and the effect on fractionation of lignocellulosic biomass and biochemical methane potential (BMP), was evaluated. Scanning electron microscopy (SEM) and IR spectroscopy were used to investigate the changes in the structural characteristics of the pretreated WSD. Fungal pretreatment results revealed that A. biennis is more attractive, since it resulted in higher lignin degradation and lower holocellulose uptake. Samples of the 14th and 30th d of cultivation (i.e. the middle and the end of the pretreatment experiment) with both fungi were used for BMP tests and the effect of pretreatment duration was also evaluated. BMP increase by 31 and 43% was obtained due to the cultivation of WSD with A. biennis, for 14 and 30 d, respectively. In addition, combination of biological (after 30 d of cultivation) with alkaline (NaOH 20 g/100 gTS) pretreatment was performed, in order to assess the effect of the chemical agent on biologically pretreated WSD, in terms of lignocellulosic content and BMP. Combination of alkaline with fungal pretreatment led to high lignin degradation for both fungi, while the cellulose and hemicellulose removal efficiencies were higher for combined alkaline and L. menziesii pretreatment. The maximum BMP was observed for the combined alkaline and A. biennis pretreatment and was 12.5 and 50.1% higher than the respective alkaline and fungal pretreatment alone and 115% higher than the respective BMP of raw WSD.

ACS Style

Maria Alexandropoulou; Georgia Antonopoulou; Efsevia Fragkou; Ioanna Ntaikou; Gerasimos Lyberatos. Fungal pretreatment of willow sawdust and its combination with alkaline treatment for enhancing biogas production. Journal of Environmental Management 2017, 203, 704 -713.

AMA Style

Maria Alexandropoulou, Georgia Antonopoulou, Efsevia Fragkou, Ioanna Ntaikou, Gerasimos Lyberatos. Fungal pretreatment of willow sawdust and its combination with alkaline treatment for enhancing biogas production. Journal of Environmental Management. 2017; 203 ():704-713.

Chicago/Turabian Style

Maria Alexandropoulou; Georgia Antonopoulou; Efsevia Fragkou; Ioanna Ntaikou; Gerasimos Lyberatos. 2017. "Fungal pretreatment of willow sawdust and its combination with alkaline treatment for enhancing biogas production." Journal of Environmental Management 203, no. : 704-713.

Journal article
Published: 17 January 2017 in Sustainability
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In this study the effect of nitrogen (N) supplementation in the fungal pretreatment of willow sawdust (WSD) via the white rot fungus Abortiporus biennis (A. biennis) was studied in terms of the fractionation of lignocellulosic biomass and biochemical methane potential (BMP). Thus, different external nitrogen sources (yeast extract (YE), urea (UR), and ammonium nitrate (AN)) at different ratios (N/C of 1/250 and 1/50) were added and the effect of the above parameters on the chemical composition of WSD during solid-state fermentation (SSF) experiments with A. biennis was assessed and compared to the experiment of fungal pretreatment without N supplementation (N/C was 1/500, control experiment). The results indicated that the addition of an external nitrogen source did not facilitate delignification, regardless of the type of nitrogen source and the ratio of N/C used. On the other hand, enhanced cellulose uptake was observed. Samples of the 28th day of cultivation, with and without N supplementation, were used for BMP tests, where a reduction in methane yield was observed, compared to the control experiment. In addition, a combination of fungal with alkali (20% NaOH w/w dry mass) pretreatment was performed in order to assess the effect of combined pretreatment on the lignocellulosic content and the BMP.

ACS Style

Maria Alexandropoulou; Georgia Antonopoulou; Ioanna Ntaikou; Gerasimos Lyberatos. Fungal Pretreatment of Willow Sawdust with Abortiporus biennis for Anaerobic Digestion: Impact of an External Nitrogen Source. Sustainability 2017, 9, 130 .

AMA Style

Maria Alexandropoulou, Georgia Antonopoulou, Ioanna Ntaikou, Gerasimos Lyberatos. Fungal Pretreatment of Willow Sawdust with Abortiporus biennis for Anaerobic Digestion: Impact of an External Nitrogen Source. Sustainability. 2017; 9 (1):130.

Chicago/Turabian Style

Maria Alexandropoulou; Georgia Antonopoulou; Ioanna Ntaikou; Gerasimos Lyberatos. 2017. "Fungal Pretreatment of Willow Sawdust with Abortiporus biennis for Anaerobic Digestion: Impact of an External Nitrogen Source." Sustainability 9, no. 1: 130.

Journal article
Published: 01 January 2017 in Journal of Hazardous Materials
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The present study evaluates a battery of marine species-based bioassays against chemically characterized municipal wastewater samples (raw and WWTP treated). We estimated Dunaliella tertiolecta growth rate inhibition (24-96h IC values), Artemia franciscana immobilization (24h LC values), mussel hemocytes viability and lipid peroxidation enhancement (in terms of neutral red retention assay/NRRT and malondialdehyde/MDA content, respectively) in influent- and WWTP effluent-treated species. We found algal growth arrest and stimulation respectively, almost similar 24hLC values in Artemia sp., and significantly higher adverse effects (in terms of NRRT and MDA levels) in influent-treated mussel hemocytes. Furthermore, the estimation of hatchability, yolk-sac larvae mortality (24-120hLC) and spinal deformities (SD) in sea bream Sparus aurata showed slight variations over time, with the lowest LC and SD (representing spinal deformities at 50% of yolk-sac larvae) values to be observed in influent-treated larvae at 120h. Data interpretation (both chemical and biological) revealed that toxic endpoints, such as NRRT, 96hICDun, 120hLCSparus and 120hSDSparus, significantly related to WWTP removal efficiency and further mediated by the presence of dominant compounds, such as As and Cr, could be used for identifying main components of toxicity in wastewaters.

ACS Style

Kassiani Harbi; Pavlos Makridis; Christos Koukoumis; Marina Papadionysiou; Theodoros Vgenis; Michael Kornaros; Ioanna Ntaikou; Sinos Giokas; Stefanos Dailianis. Evaluation of a battery of marine species-based bioassays against raw and treated municipal wastewaters. Journal of Hazardous Materials 2017, 321, 537 -546.

AMA Style

Kassiani Harbi, Pavlos Makridis, Christos Koukoumis, Marina Papadionysiou, Theodoros Vgenis, Michael Kornaros, Ioanna Ntaikou, Sinos Giokas, Stefanos Dailianis. Evaluation of a battery of marine species-based bioassays against raw and treated municipal wastewaters. Journal of Hazardous Materials. 2017; 321 ():537-546.

Chicago/Turabian Style

Kassiani Harbi; Pavlos Makridis; Christos Koukoumis; Marina Papadionysiou; Theodoros Vgenis; Michael Kornaros; Ioanna Ntaikou; Sinos Giokas; Stefanos Dailianis. 2017. "Evaluation of a battery of marine species-based bioassays against raw and treated municipal wastewaters." Journal of Hazardous Materials 321, no. : 537-546.