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Nesterenkonia sp. PF2B19, a psychrophile was isolated from 44,800-year-old permafrost soil. This is the first report on comparative genomics of Nesterenkonia sp. isolated from Arctic. Genome of PF2B19 exhibited the presence of a vast array of genetic determinants involved in cold adaptation i.e., response to cold-associated general, osmotic, and oxidative stress. These genomic attributes proved to be valuable in unraveling the adaptive tactics employed by PF2B19 for survival in the cold permafrost soils of the Arctic. Genomic analysis of PF2B19 has given some valuable insight into the biotechnological potential of this strain, particularly as a source of cold-active enzymes, as a bioremediating agent and as plant growth-promoting bacteria.
Purnima Singh; Neelam Kapse; Vasudevan Gowdaman; Masaharu Tsuji; Shiv Singh; Prashant Dhakephalkar. Comparative Genomic Analysis of Arctic Permafrost Bacterium Nesterenkonia sp. PF2B19 to Gain Insights into Its Cold Adaptation Tactic and Diverse Biotechnological Potential. Sustainability 2021, 13, 4590 .
AMA StylePurnima Singh, Neelam Kapse, Vasudevan Gowdaman, Masaharu Tsuji, Shiv Singh, Prashant Dhakephalkar. Comparative Genomic Analysis of Arctic Permafrost Bacterium Nesterenkonia sp. PF2B19 to Gain Insights into Its Cold Adaptation Tactic and Diverse Biotechnological Potential. Sustainability. 2021; 13 (8):4590.
Chicago/Turabian StylePurnima Singh; Neelam Kapse; Vasudevan Gowdaman; Masaharu Tsuji; Shiv Singh; Prashant Dhakephalkar. 2021. "Comparative Genomic Analysis of Arctic Permafrost Bacterium Nesterenkonia sp. PF2B19 to Gain Insights into Its Cold Adaptation Tactic and Diverse Biotechnological Potential." Sustainability 13, no. 8: 4590.
To understand the microbial composition and diversity patterns, cryoconite granules were collected from two geographical areas, i.e., Nepali Himalaya and Greenland, Arctic. 16S rRNA, ITS and the D1/D2 domain sequencing techniques were used for characterization of microbial communities of the four glaciers. The total 13 species of bacteria such as Bacillus aryabhattai, Bacillus simplex, Brevundimonas vesicularis, Cryobacterium luteum, Cryobacterium psychrotolerans, Dermacoccus nishinomiyaensis, Glaciihabitans tibetensis, Leifsonia kafniensis, Paracoccus limosus, Polaromonas glacialis, Sporosarcina globispora, Staphylococcus saprophyticus, Variovorax ginsengisoli, and 4 species of fungi such as Goffeauzyma gilvescens, Mrakia robertii, Dothideomycetes sp., Helotiales sp. were recorded from Nepali Himalaya. Among these, 12 species of bacteria and 4 species of fungi are new contributions to Himalaya. In contrast to this, six species of bacteria such as Bacillus cereus, Cryobacterium psychrotolerans, Dermacoccus nishinomiyaensis, Enhydrobacter aerosaccus, Glaciihabitans tibetensis, Subtercola frigoramans, and nine species of fungi such as Goffeauzyma gilvescens, Mrakia robertii, Naganishia vaughanmartiniae, Piskurozyma fildesensis, Rhodotorula svalbardensis, Alatospora acuminata, Articulospora sp., Phialophora sp., Thelebolus microspores, and Dothideomycetes sp.), were recorded from Qaanaaq, Isunnguata Sermia and Thule glaciers, Greenland. Among these, five species of bacteria and seven species of fungi are new contributions to Greenland cryoconite. Microbial analyses indicate that the Nepali Himalayan cryoconite colonize higher numbers of microbial species compared to the Greenland cryoconite.
Purnima Singh; Masaharu Tsuji; Shiv Mohan Singh; Nozomu Takeuchi. Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic. Sustainability 2020, 12, 6477 .
AMA StylePurnima Singh, Masaharu Tsuji, Shiv Mohan Singh, Nozomu Takeuchi. Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic. Sustainability. 2020; 12 (16):6477.
Chicago/Turabian StylePurnima Singh; Masaharu Tsuji; Shiv Mohan Singh; Nozomu Takeuchi. 2020. "Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic." Sustainability 12, no. 16: 6477.
Basidiomycetous yeast species of the genus Mrakia have been reported from a variety of extreme cold environments such as polar regions, glaciers, and high mountains. Several reports indicate that fungal species within the genus Mrakia occupy a major mycoflora in Antarctic environments. These results strongly suggest that this genus is well adapted to the polar environment. The genus Mrakia has unique characteristics such as an ethanol fermentation ability, and the ability to decompose milk fat under low-temperature conditions. Thus, the genus Mrakia has quite interesting characteristics. We believe that the results obtained in previous studies will contribute to the progress of related research fields and hope that further investigation will offer many opportunities to obtain more valuable knowledge on Antarctic microbes and their potential uses for human activities. In this chapter, we review the taxonomic history, physiology, ecological role, and biotechnological applications of basidiomycetous yeasts within the genus Mrakia. In the near future, this genus will become an important agent in the field of low-temperature microbiology.
Masaharu Tsuji; Sakae Kudoh; Yukiko Tanabe; Tamotsu Hoshino. Basidiomycetous Yeast of the Genus Mrakia. Fungi in Extreme Environments: Ecological Role and Biotechnological Significance 2019, 145 -156.
AMA StyleMasaharu Tsuji, Sakae Kudoh, Yukiko Tanabe, Tamotsu Hoshino. Basidiomycetous Yeast of the Genus Mrakia. Fungi in Extreme Environments: Ecological Role and Biotechnological Significance. 2019; ():145-156.
Chicago/Turabian StyleMasaharu Tsuji; Sakae Kudoh; Yukiko Tanabe; Tamotsu Hoshino. 2019. "Basidiomycetous Yeast of the Genus Mrakia." Fungi in Extreme Environments: Ecological Role and Biotechnological Significance , no. : 145-156.
A psychrotolerant yeast strain Mrakia robertii A2‐3 isolated from cryoconites of Hamtah glacier, Himalaya, India was investigated for the production of cold tolerant endoglucanase. Optimum endoglucanase production was found at 15 °C with an initial pH of 5.5, and potent inducers were 1% w/v of xylose and KNO3 and 0.1% w/v of NaCl. Under the optimum conditions, enzyme production was 1.81 fold higher than the un‐optimized conditions. Crude enzyme was partially purified by ammonium sulphate precipitation followed by dialysis. The enzyme was purified to 2.53 fold and yield was 6.03% with specific activity of 17.38 U/mg and molecular weight ~57 kDa. The Km and Vmax values of the partially purified enzyme were found to be 1.57 mg/ml and 142.85 U/mg, respectively. The characterization study revealed that the best temperature was 15°C for activity and stability. Furthermore enzyme showed the highest activity at pH 11.0 and was stable at pH6.0. Fe2+, Mn2+, Na2+, Cu2+, Co2+, Ca2+ proved to be activators of endoglucanase. EDTA showed very low effect on the enzyme activity whereas it was active with Tween‐80 and sodium deoxycholate. The present study successfully produced a cold‐active endoglucanase with novel properties making it promising as a biocatalyst for industrial processes. This article is protected by copyright. All rights reserved.
Gandhali M. Dhume; Abhas K. Maharana; Masaharu Tsuji; Alok K. Srivastava; Shiv Mohan Singh. Cold-tolerant endoglucanase producing ability of Mrakia robertii A2-3 isolated from cryoconites, Hamtha glacier, Himalaya. Journal of Basic Microbiology 2019, 59, 667 -679.
AMA StyleGandhali M. Dhume, Abhas K. Maharana, Masaharu Tsuji, Alok K. Srivastava, Shiv Mohan Singh. Cold-tolerant endoglucanase producing ability of Mrakia robertii A2-3 isolated from cryoconites, Hamtha glacier, Himalaya. Journal of Basic Microbiology. 2019; 59 (7):667-679.
Chicago/Turabian StyleGandhali M. Dhume; Abhas K. Maharana; Masaharu Tsuji; Alok K. Srivastava; Shiv Mohan Singh. 2019. "Cold-tolerant endoglucanase producing ability of Mrakia robertii A2-3 isolated from cryoconites, Hamtha glacier, Himalaya." Journal of Basic Microbiology 59, no. 7: 667-679.
Masaharu Tsuji; Tamotsu Hoshino. Ethanol Fermentation by the Basidiomycetous Yeast Mrakia blollopis Under Low Temperature Conditions. Fungi in Polar Regions 2019, 120 -132.
AMA StyleMasaharu Tsuji, Tamotsu Hoshino. Ethanol Fermentation by the Basidiomycetous Yeast Mrakia blollopis Under Low Temperature Conditions. Fungi in Polar Regions. 2019; ():120-132.
Chicago/Turabian StyleMasaharu Tsuji; Tamotsu Hoshino. 2019. "Ethanol Fermentation by the Basidiomycetous Yeast Mrakia blollopis Under Low Temperature Conditions." Fungi in Polar Regions , no. : 120-132.
Masaharu Tsuji. An Index of Non-Lichenized Fungi Recorded in the Vicinity of Syowa Station, East Antarctica. Fungi in Polar Regions 2019, 1 -16.
AMA StyleMasaharu Tsuji. An Index of Non-Lichenized Fungi Recorded in the Vicinity of Syowa Station, East Antarctica. Fungi in Polar Regions. 2019; ():1-16.
Chicago/Turabian StyleMasaharu Tsuji. 2019. "An Index of Non-Lichenized Fungi Recorded in the Vicinity of Syowa Station, East Antarctica." Fungi in Polar Regions , no. : 1-16.
Four strains isolated from sediment sampled at the front of a retreating glacier on northern Ellesmere Island in the Canadian high Arctic, namely JCM 32575T, JCM 32576, JCM 32577 and JCM 32578, belong to a novel psychrophilic basidiomycetous yeast species in the genus Mrakia. Molecular phylogenetic analysis indicated that these strains are most closely related to the type strains of Mrakia aquatica and Mrakianic combsii, but with 8–9 and 7–12 nt substitutions in ITS and in the D1/D2 domain of the LSU rRNA gene, respectively. The strains grew at sub-zero temperatures and in vitamin-free media, with lipase and cellulase highly active even at −3 °C. These characteristics likely allow this yeast species to grow and survive in extremely cold, oligotrophic environments, such as the fronts of retreating glaciers in the high Arctic. The name Mrakia hoshinonis sp. nov. is proposed, with type strain JCM 32575T (UAMH 11969) and MycoBank number MB 825484.
Masaharu Tsuji; Yukiko Tanabe; Warwick Vincent; Masaki Uchida. Mrakia hoshinonis sp. nov., a novel psychrophilic yeast isolated from a retreating glacier on Ellesmere Island in the Canadian High Arctic. International Journal of Systematic and Evolutionary Microbiology 2019, 69, 944 -948.
AMA StyleMasaharu Tsuji, Yukiko Tanabe, Warwick Vincent, Masaki Uchida. Mrakia hoshinonis sp. nov., a novel psychrophilic yeast isolated from a retreating glacier on Ellesmere Island in the Canadian High Arctic. International Journal of Systematic and Evolutionary Microbiology. 2019; 69 (4):944-948.
Chicago/Turabian StyleMasaharu Tsuji; Yukiko Tanabe; Warwick Vincent; Masaki Uchida. 2019. "Mrakia hoshinonis sp. nov., a novel psychrophilic yeast isolated from a retreating glacier on Ellesmere Island in the Canadian High Arctic." International Journal of Systematic and Evolutionary Microbiology 69, no. 4: 944-948.
Two strains of a psychrophilic basidiomycetous yeast species belonging to the genus Vishniacozyma were isolated from sediments and soil at the front of a retreating glacier on northern Ellesmere Island in the Canadian Arctic. Analysis of the large subunit D1/D2 region and the internal transcribed spacer (ITS) regions of the rDNA indicated that these strains represented a novel species. The closest relatives of the novel species were Vishniacozyma globispora and V. dimennae, which exhibited sequence similarities of 82.2 and 81.6 %, respectively, to the ITS region of the novel species, and contained 61 and 57 nt substitutions, respectively, in the D1/D2 domain, in comparison with the novel species. Strains of the novel species were able to grow at sub-zero temperatures and in vitamin-free medium. These characteristics allow the strains to survive and grow in perennially cold, oligotrophic habitats in the Canadian High Arctic. The name Vishniacozyma ellesmerensis sp. nov. is proposed. The type strain is JCM 32573T (=UAMH 11973T=G3-4-8T) and the MycoBank number is MB825501.
Masaharu Tsuji; Yukiko Tanabe; Warwick Vincent; Masaki Uchida. Vishniacozyma ellesmerensis sp. nov., a psychrophilic yeast isolated from a retreating glacier in the Canadian High Arctic. International Journal of Systematic and Evolutionary Microbiology 2019, 69, 696 -700.
AMA StyleMasaharu Tsuji, Yukiko Tanabe, Warwick Vincent, Masaki Uchida. Vishniacozyma ellesmerensis sp. nov., a psychrophilic yeast isolated from a retreating glacier in the Canadian High Arctic. International Journal of Systematic and Evolutionary Microbiology. 2019; 69 (3):696-700.
Chicago/Turabian StyleMasaharu Tsuji; Yukiko Tanabe; Warwick Vincent; Masaki Uchida. 2019. "Vishniacozyma ellesmerensis sp. nov., a psychrophilic yeast isolated from a retreating glacier in the Canadian High Arctic." International Journal of Systematic and Evolutionary Microbiology 69, no. 3: 696-700.
Tamotsu Hoshino; Masaharu Tsuji. 低温排水処理に適した南極酵母の新たな生きざま. KAGAKU TO SEIBUTSU 2018, 56, 586 -588.
AMA StyleTamotsu Hoshino, Masaharu Tsuji. 低温排水処理に適した南極酵母の新たな生きざま. KAGAKU TO SEIBUTSU. 2018; 56 (9):586-588.
Chicago/Turabian StyleTamotsu Hoshino; Masaharu Tsuji. 2018. "低温排水処理に適した南極酵母の新たな生きざま." KAGAKU TO SEIBUTSU 56, no. 9: 586-588.
The Japanese Antarctic Research Expedition (JARE) was started in 1957. The expedition marked its 60th anniversary in January 2017. In total 76 fungal species (61 ascomycetous fungi, including 9 unidentified species, and 16 basidiomycetous fungi) have thus far been recorded from the area around Syowa Station. In this review, I present a catalog of the fungal species isolated from the vicinity of Syowa Station to mark the 60th anniversary of JARE.
Masaharu Tsuji. A catalog of fungi recorded from the vicinity of Syowa Station. Mycoscience 2018, 59, 319 -324.
AMA StyleMasaharu Tsuji. A catalog of fungi recorded from the vicinity of Syowa Station. Mycoscience. 2018; 59 (4):319-324.
Chicago/Turabian StyleMasaharu Tsuji. 2018. "A catalog of fungi recorded from the vicinity of Syowa Station." Mycoscience 59, no. 4: 319-324.
Masaharu Tsuji; Yukiko Tanabe; Warwick Vincent; Masaki Uchida. Mrakia arctica sp. nov., a new psychrophilic yeast isolated from an ice island in the Canadian High Arctic. Mycoscience 2018, 59, 54 -58.
AMA StyleMasaharu Tsuji, Yukiko Tanabe, Warwick Vincent, Masaki Uchida. Mrakia arctica sp. nov., a new psychrophilic yeast isolated from an ice island in the Canadian High Arctic. Mycoscience. 2018; 59 (1):54-58.
Chicago/Turabian StyleMasaharu Tsuji; Yukiko Tanabe; Warwick Vincent; Masaki Uchida. 2018. "Mrakia arctica sp. nov., a new psychrophilic yeast isolated from an ice island in the Canadian High Arctic." Mycoscience 59, no. 1: 54-58.
Masaharu Tsuji; Yukiko Tanabe; Warwick Vincent; Masaki Uchida. Gelidatrema psychrophila sp. nov., a novel yeast species isolated from an ice island in the Canadian High Arctic. Mycoscience 2018, 59, 67 -70.
AMA StyleMasaharu Tsuji, Yukiko Tanabe, Warwick Vincent, Masaki Uchida. Gelidatrema psychrophila sp. nov., a novel yeast species isolated from an ice island in the Canadian High Arctic. Mycoscience. 2018; 59 (1):67-70.
Chicago/Turabian StyleMasaharu Tsuji; Yukiko Tanabe; Warwick Vincent; Masaki Uchida. 2018. "Gelidatrema psychrophila sp. nov., a novel yeast species isolated from an ice island in the Canadian High Arctic." Mycoscience 59, no. 1: 67-70.
Microbes growing at low temperatures encounter numerous growth constraints. However, fungi that inhabit polar regions can grow and decompose organic compounds at low temperatures. Research on the biodiversity and secretion ability of yeasts from East Ongul Island, and evaluation of their extracellular enzymatic activity at temperatures ranging from −3 to 20 °C, was performed and described in this study. In total, 95 yeast strains were isolated from the surface soil on East Ongul Island, East Antarctica. Based on the internal transcribed spacer (ITS) region and 26S rDNA D1/D2 domain sequence similarity, these strains were classified into 10 genera and 16 species. All strains were categorized as basidiomycetous yeasts. One representative strain was selected from each species, and its ability to grow at temperatures between −3 and 37 °C was evaluated. Strain abilities to secrete extracellular enzymes (lipase, cellulase, and protease) at temperatures ranging from −3 to 20 °C were also investigated. Based on the optimal and maximal growth temperatures, the yeasts were classified as psychrophilic or psychrotolerant. All the strains investigated in this study were able to grow at −3 °C and in vitamin-free media. Psychrophilic yeasts, such as Glaciozyma antarctica, Gl. martinii, Mrakia gelida, and Phenoliferia glacialis, did not secrete pronounced amounts of extracellular enzymes at −3 °C; in contrast, the psychrotolerant yeasts Goffeauzyma gilvescens, Holtermanniella wattica, Tausonia pullulans, and Udeniomyces puniceus secreted copious amounts of enzymes, even at subzero temperatures. These results indicated that psychrotolerant yeasts, rather than psychrophilic yeasts, play important roles in the nutrient cycle in the East Ongul Island ecosystem at near-subzero temperatures.
Masaharu Tsuji. Genetic diversity of yeasts from East Ongul Island, East Antarctica and their extracellular enzymes secretion. Polar Biology 2017, 41, 249 -258.
AMA StyleMasaharu Tsuji. Genetic diversity of yeasts from East Ongul Island, East Antarctica and their extracellular enzymes secretion. Polar Biology. 2017; 41 (2):249-258.
Chicago/Turabian StyleMasaharu Tsuji. 2017. "Genetic diversity of yeasts from East Ongul Island, East Antarctica and their extracellular enzymes secretion." Polar Biology 41, no. 2: 249-258.
Masaharu Tsuji; Megumu Tsujimoto; Satoshi Imura. Cystobasidium tubakii and Cystobasidium ongulense , new basidiomycetous yeast species isolated from East Ongul Island, East Antarctica. Mycoscience 2017, 58, 103 -110.
AMA StyleMasaharu Tsuji, Megumu Tsujimoto, Satoshi Imura. Cystobasidium tubakii and Cystobasidium ongulense , new basidiomycetous yeast species isolated from East Ongul Island, East Antarctica. Mycoscience. 2017; 58 (2):103-110.
Chicago/Turabian StyleMasaharu Tsuji; Megumu Tsujimoto; Satoshi Imura. 2017. "Cystobasidium tubakii and Cystobasidium ongulense , new basidiomycetous yeast species isolated from East Ongul Island, East Antarctica." Mycoscience 58, no. 2: 103-110.
Masaharu Tsuji; Jun Uetake; Yukiko Tanabe. Changes in the fungal community of Austre Brøggerbreen deglaciation area, Ny-Ålesund, Svalbard, High Arctic. Mycoscience 2016, 57, 448 -451.
AMA StyleMasaharu Tsuji, Jun Uetake, Yukiko Tanabe. Changes in the fungal community of Austre Brøggerbreen deglaciation area, Ny-Ålesund, Svalbard, High Arctic. Mycoscience. 2016; 57 (6):448-451.
Chicago/Turabian StyleMasaharu Tsuji; Jun Uetake; Yukiko Tanabe. 2016. "Changes in the fungal community of Austre Brøggerbreen deglaciation area, Ny-Ålesund, Svalbard, High Arctic." Mycoscience 57, no. 6: 448-451.
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions.
Masaharu Tsuji. Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis. Royal Society Open Science 2016, 3, 160106 .
AMA StyleMasaharu Tsuji. Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis. Royal Society Open Science. 2016; 3 (7):160106.
Chicago/Turabian StyleMasaharu Tsuji. 2016. "Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis." Royal Society Open Science 3, no. 7: 160106.
Masaharu Tsuji; Yuji Yokota; Kodai Shimohara; Sakae Kudoh; Tamotsu Hoshino. - Abundance, Diversity and Seasonal Dynamics of Predatory Bacteria in Aquaculture Zero Discharge Systems. Bioremediation of Wastewater 2016, 204 -231.
AMA StyleMasaharu Tsuji, Yuji Yokota, Kodai Shimohara, Sakae Kudoh, Tamotsu Hoshino. - Abundance, Diversity and Seasonal Dynamics of Predatory Bacteria in Aquaculture Zero Discharge Systems. Bioremediation of Wastewater. 2016; ():204-231.
Chicago/Turabian StyleMasaharu Tsuji; Yuji Yokota; Kodai Shimohara; Sakae Kudoh; Tamotsu Hoshino. 2016. "- Abundance, Diversity and Seasonal Dynamics of Predatory Bacteria in Aquaculture Zero Discharge Systems." Bioremediation of Wastewater , no. : 204-231.
Masaharu Tsuji; Sakae Kudoh; Tamotsu Hoshino. Ethanol productivity of cryophilic basidiomycetous yeast Mrakia spp. correlates with ethanol tolerance. Mycoscience 2016, 57, 42 -50.
AMA StyleMasaharu Tsuji, Sakae Kudoh, Tamotsu Hoshino. Ethanol productivity of cryophilic basidiomycetous yeast Mrakia spp. correlates with ethanol tolerance. Mycoscience. 2016; 57 (1):42-50.
Chicago/Turabian StyleMasaharu Tsuji; Sakae Kudoh; Tamotsu Hoshino. 2016. "Ethanol productivity of cryophilic basidiomycetous yeast Mrakia spp. correlates with ethanol tolerance." Mycoscience 57, no. 1: 42-50.
Milk fat curdle is difficult to remove from sewage. In an attempt to identify an appropriate agent for bio-remediation of milk fat curdle, Mrakia strains were collected from the Skarvsnes ice-free area of Antarctica. A total of 27 strains were isolated and tested for their ability to decompose milk fat at temperatures ranging from 4°C to 15°C. All strains could decompose milk fat at 4°C and 10°C. Phylogenetic analysis and comparison of the decomposition ability of milk fat (DAMF) revealed that the DAMF may be useful for predicting the outcome of phylogenetic analysis based on ITS sequences.
Masaharu Tsuji; Yuji Yokota; Sakae Kudoh; Tamotsu Hoshino. Comparative analysis of milk fat decomposition activity by Mrakia spp. isolated from Skarvsnes ice-free area, East Antarctica. Cryobiology 2015, 70, 293 -296.
AMA StyleMasaharu Tsuji, Yuji Yokota, Sakae Kudoh, Tamotsu Hoshino. Comparative analysis of milk fat decomposition activity by Mrakia spp. isolated from Skarvsnes ice-free area, East Antarctica. Cryobiology. 2015; 70 (3):293-296.
Chicago/Turabian StyleMasaharu Tsuji; Yuji Yokota; Sakae Kudoh; Tamotsu Hoshino. 2015. "Comparative analysis of milk fat decomposition activity by Mrakia spp. isolated from Skarvsnes ice-free area, East Antarctica." Cryobiology 70, no. 3: 293-296.
Mrakia blollopis strain SK-4 was isolated from an algal mat of Naga-ike, a lake in Skarvsnes, East Antarctica. Here, we report the draft genome sequence of M. blollopis SK-4. This is the first report on the genome sequence of any cold-adapted fungal species.
Masaharu Tsuji; Sakae Kudoh; Tamotsu Hoshino; Janne Isojärvi; Sumathy Shunmugam; Kaarina Sivonen; Yagut Allahverdiyeva; Eva-Mari Aro; Natalia Battchikova. Draft Genome Sequence of Cryophilic Basidiomycetous Yeast Mrakia blollopis SK-4, Isolated from an Algal Mat of Naga-ike Lake in the Skarvsnes Ice-Free Area, East Antarctica. Genome Announcements 2015, 3, e01474-14 .
AMA StyleMasaharu Tsuji, Sakae Kudoh, Tamotsu Hoshino, Janne Isojärvi, Sumathy Shunmugam, Kaarina Sivonen, Yagut Allahverdiyeva, Eva-Mari Aro, Natalia Battchikova. Draft Genome Sequence of Cryophilic Basidiomycetous Yeast Mrakia blollopis SK-4, Isolated from an Algal Mat of Naga-ike Lake in the Skarvsnes Ice-Free Area, East Antarctica. Genome Announcements. 2015; 3 (1):e01474-14.
Chicago/Turabian StyleMasaharu Tsuji; Sakae Kudoh; Tamotsu Hoshino; Janne Isojärvi; Sumathy Shunmugam; Kaarina Sivonen; Yagut Allahverdiyeva; Eva-Mari Aro; Natalia Battchikova. 2015. "Draft Genome Sequence of Cryophilic Basidiomycetous Yeast Mrakia blollopis SK-4, Isolated from an Algal Mat of Naga-ike Lake in the Skarvsnes Ice-Free Area, East Antarctica." Genome Announcements 3, no. 1: e01474-14.