This page has only limited features, please log in for full access.

Unclaimed
Jianhua Wang
Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China

Basic Info

Basic Info is private.

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Book chapter
Published: 07 October 2020 in Mycotoxins and Food Safety
Reads 0
Downloads 0

The fungal phytopathogen in Fusarium species can cause Fusarium head blight of wheat, barley, oats, and other small cereal grain crops worldwide. Most importantly, these fungi can produce different kinds of mycoxins, and they are harmful to humans and animal health. FAO reported that approximately 25% of the world’s grains were contaminated by mycotoxins annually. This chapter will focus on several topics as below: (1) composition of Fusarium graminearum species complex; (2) genotype determination of Fusarium graminearum species complex strains from different hosts and their population structure changes; (3) genetic approaches to genotype determination in type B-trichothecene producing Fusaria fungi; and (4) some newly identified trichothecene mycotoxins, their toxicity, and distribution of the producers.

ACS Style

Jianhua Wang; Zhiyong Zhao; Xianli Yang; Junhua Yang; Andong Gong; Jingya Zhang; Lei Chen; Changyan Zhou. Fusarium graminearum Species Complex and Trichothecene Genotype. Mycotoxins and Food Safety 2020, 1 .

AMA Style

Jianhua Wang, Zhiyong Zhao, Xianli Yang, Junhua Yang, Andong Gong, Jingya Zhang, Lei Chen, Changyan Zhou. Fusarium graminearum Species Complex and Trichothecene Genotype. Mycotoxins and Food Safety. 2020; ():1.

Chicago/Turabian Style

Jianhua Wang; Zhiyong Zhao; Xianli Yang; Junhua Yang; Andong Gong; Jingya Zhang; Lei Chen; Changyan Zhou. 2020. "Fusarium graminearum Species Complex and Trichothecene Genotype." Mycotoxins and Food Safety , no. : 1.

Journal article
Published: 10 January 2020 in Toxins
Reads 0
Downloads 0

T-2 toxin, as a highly toxic mycotoxin to humans and animals, induces oxidative stress and apoptosis in various cells and tissues. Apoptosis and mitochondrial fusion/fission are two tightly interconnected processes that are crucial for maintaining physiological homeostasis. However, the role of mitochondrial fusion/fission in apoptosis of T-2 toxin remains unknown. Hence, we aimed to explore the putative role of mitochondrial fusion/fission on T-2 toxin induced apoptosis in normal human liver (HL-7702) cells. T-2 toxin treatment (0, 0.1, 1.0, or 10 μg/L) for 24 h caused decreased cell viability and ATP concentration and increased production of (ROS), as seen by a loss of mitochondrial membrane potential (∆Ψm) and increase in mitochondrial fragmentation. Subsequently, the mitochondrial dynamic imbalance was activated, evidenced by a dose-dependent decrease and increase in the protein expression of mitochondrial fusion (OPA1, Mfn1, and Mfn2) and fission (Drp1 and Fis1), respectively. Furthermore, the T-2 toxin promoted the release of cytochrome c from mitochondria to cytoplasm and induced cell apoptosis triggered by upregulation of Bax and Bax/Bcl-2 ratios, and further activated the caspase pathways. Taken together, these results indicate that altered mitochondrial dynamics induced by oxidative stress with T-2 toxin exposure likely contribute to mitochondrial injury and HL-7702 cell apoptosis.

ACS Style

Junhua Yang; Wenbo Guo; Jianhua Wang; Xianli Yang; Zhiqi Zhang; Zhihui Zhao. T-2 Toxin-Induced Oxidative Stress Leads to Imbalance of Mitochondrial Fission and Fusion to Activate Cellular Apoptosis in the Human Liver 7702 Cell Line. Toxins 2020, 12, 43 .

AMA Style

Junhua Yang, Wenbo Guo, Jianhua Wang, Xianli Yang, Zhiqi Zhang, Zhihui Zhao. T-2 Toxin-Induced Oxidative Stress Leads to Imbalance of Mitochondrial Fission and Fusion to Activate Cellular Apoptosis in the Human Liver 7702 Cell Line. Toxins. 2020; 12 (1):43.

Chicago/Turabian Style

Junhua Yang; Wenbo Guo; Jianhua Wang; Xianli Yang; Zhiqi Zhang; Zhihui Zhao. 2020. "T-2 Toxin-Induced Oxidative Stress Leads to Imbalance of Mitochondrial Fission and Fusion to Activate Cellular Apoptosis in the Human Liver 7702 Cell Line." Toxins 12, no. 1: 43.

Applied microbial and cell physiology
Published: 22 June 2019 in Applied Microbiology and Biotechnology
Reads 0
Downloads 0

Fumonisin B1 (FB1) contamination in cereals and cereal products remains an important aspect of food safety because of its wide distribution and the potential health hazard. However, only a few microorganisms have been reported to effectively degrade FB1. In this present study, a bacterial consortium SAAS79 with highly FB1-degrading activity was isolated from the spent mushroom compost. The combination of antibiotic-driven selection and 16S rDNA sequencing identified the Pseudomonas genus as the key FB1-degrading member. The microbial consortium could degrade more than 90% of 10 μg/mL FB1 after incubation for 24 h at pH of 5-7 and temperature of 28-35 °C. The enzymes from the intracellular space were proved to be responsible for FB1 degradation, which eliminated about 90% of 10 μg/mL FB1 in 3 h. Besides, liquid chromatography time-of-flight mass spectrometry (LC-TOF/MS) analysis identified two degradation products of FB1, and their toxicity on the monkey kidney cells (MARC-145) was significantly lower (p < 0.05) compared with the parent FB1. Overall, the consortium SAAS79 and its crude enzymes may be a potential choice for the decontamination of FB1 in the feed and food industry. Also, the bacterial consortium provides a new source of genes for the development of enzymatic detoxification agent.

ACS Style

Zhiyong Zhao; Yanmei Zhang; Andong Gong; Na Liu; Shanshan Chen; Xiaoyan Zhao; Xiaobei Li; Lei Chen; Changyan Zhou; Jianhua Wang. Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79. Applied Microbiology and Biotechnology 2019, 103, 7129 -7140.

AMA Style

Zhiyong Zhao, Yanmei Zhang, Andong Gong, Na Liu, Shanshan Chen, Xiaoyan Zhao, Xiaobei Li, Lei Chen, Changyan Zhou, Jianhua Wang. Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79. Applied Microbiology and Biotechnology. 2019; 103 (17):7129-7140.

Chicago/Turabian Style

Zhiyong Zhao; Yanmei Zhang; Andong Gong; Na Liu; Shanshan Chen; Xiaoyan Zhao; Xiaobei Li; Lei Chen; Changyan Zhou; Jianhua Wang. 2019. "Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79." Applied Microbiology and Biotechnology 103, no. 17: 7129-7140.

Article
Published: 03 February 2018 in Food Analytical Methods
Reads 0
Downloads 0

Fusarium species are one of the common pathogenic fungi of vegetable diseases. Some Fusarium species produce toxic secondary metabolites, which have potential health risks for humans and animals. In the study, an ultra-performance liquid chromatography tandem mass spectrometry method (UPLC-MS/MS) was developed for the analysis of 13 emerging and traditional Fusarium toxins in pepper, tomato, cucumber, and potato. Samples were extracted by a modified quick, easy, cheap, effective, rugged, and safe method. Dispersive solid-phase extraction was employed for the removal of matrix interferences from the crude extracts of various vegetables. Matrix effect was compensated using matrix-matched calibration curves. The method was fully validated according to the European Commission Decision 2002/657/EC. For all the targeted analytes, good linearity (R2 ≥ 0.9823) was obtained. Recoveries were in the range of 62 and 114% in pepper, tomato, cucumber, and potato, with intra-day relative standard deviations lower than 14.3%. Quantitation limits of the developed method were between 0.25 and 20 μg/kg in four sample matrices. The method was successfully employed for the analysis of Fusarium toxins in vegetable samples.

ACS Style

Zhiyong Zhao; Xianli Yang; Xiaoyan Zhao; Lei Chen; Bing Bai; Changyan Zhou; Jianhua Wang. Method Development and Validation for the Analysis of Emerging and Traditional Fusarium Mycotoxins in Pepper, Potato, Tomato, and Cucumber by UPLC-MS/MS. Food Analytical Methods 2018, 11, 1780 -1788.

AMA Style

Zhiyong Zhao, Xianli Yang, Xiaoyan Zhao, Lei Chen, Bing Bai, Changyan Zhou, Jianhua Wang. Method Development and Validation for the Analysis of Emerging and Traditional Fusarium Mycotoxins in Pepper, Potato, Tomato, and Cucumber by UPLC-MS/MS. Food Analytical Methods. 2018; 11 (6):1780-1788.

Chicago/Turabian Style

Zhiyong Zhao; Xianli Yang; Xiaoyan Zhao; Lei Chen; Bing Bai; Changyan Zhou; Jianhua Wang. 2018. "Method Development and Validation for the Analysis of Emerging and Traditional Fusarium Mycotoxins in Pepper, Potato, Tomato, and Cucumber by UPLC-MS/MS." Food Analytical Methods 11, no. 6: 1780-1788.

Journal article
Published: 20 September 2011 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Positive-negative PCR assays based on the genes involved in the trichothecene biosynthesis pathway are useful in assessing the risk of trichothecene contamination in grain and are important in epidemiological studies. A single PCR detection method based on the structural gene sequence of TRI13 gene has been developed to predict the 3-ADON, 15-ADON and NIV chemotypes in China. The chemotypic differences are based on the deletions within the TRI13 gene. The objective of this study was to assess the reliability of using this single primer based on the TRI13 gene to differentiate the F. graminearum chemotypes in Canada. In this study, we found that, this single PCR detection method based on the deletions in the TRI13 gene cannot be used to differentiate the 3-ADON and 15-ADON chemotypes in the Canadian F. graminearum isolates; further sequence analysis of the PCR products confirmed that both Canadian 3-ADON and 15-ADON chemotypes have the 61 bp deletion in the TRI13 gene. This 61 bp deletion was absent in the Chinese 3-ADON isolates. Therefore these findings revealed that there are genetic differences between the examined 3-ADON F. graminearum isolates from Canada and China. The observed genetic differences between the 3-ADON chemotype populations in Canada and China may be resulted from a random mutation (insertion/deletion) that took place in one of the populations and accumulated due to genetic drift and/or selection

ACS Style

Chami Amarasinghe; Jian-Hua Wang; Yu-Cai Liao; W.G. Dilantha Fernando. Difference in TRI13 Gene Sequences between the 3-Acetyldeoxynivalenol Producing Fusarium graminearum Chemotypes from Canada and China. International Journal of Molecular Sciences 2011, 12, 6164 -6175.

AMA Style

Chami Amarasinghe, Jian-Hua Wang, Yu-Cai Liao, W.G. Dilantha Fernando. Difference in TRI13 Gene Sequences between the 3-Acetyldeoxynivalenol Producing Fusarium graminearum Chemotypes from Canada and China. International Journal of Molecular Sciences. 2011; 12 (9):6164-6175.

Chicago/Turabian Style

Chami Amarasinghe; Jian-Hua Wang; Yu-Cai Liao; W.G. Dilantha Fernando. 2011. "Difference in TRI13 Gene Sequences between the 3-Acetyldeoxynivalenol Producing Fusarium graminearum Chemotypes from Canada and China." International Journal of Molecular Sciences 12, no. 9: 6164-6175.