This page has only limited features, please log in for full access.

Unclaimed
Wei Chen
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, PR China

Basic Info

Basic Info is private.

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 17 August 2021 in Microbiology
Reads 0
Downloads 0

Phenylalanine hydroxylase (PAH) catalyses the irreversible hydroxylation of phenylalanine to tyrosine, which is the rate-limiting reaction in phenylalanine metabolism in animals. A variety of polyunsaturated fatty acids can be synthesized by the lipid-producing fungus Mortierella alpina, which has a wide range of industrial applications in the production of arachidonic acid. In this study, RNA interference (RNAi) with the gene PAH was used to explore the role of phenylalanine hydroxylation in lipid biosynthesis in M. alpina. Our results indicated that PAH knockdown decreased the PAH transcript level by approximately 55% and attenuated cellular fatty acid biosynthesis. Furthermore, the level of NADPH, which is a critical reducing agent and the limiting factor in lipogenesis, was decreased in response to PAH RNAi, in addition to the downregulated transcription of other genes involved in NADPH production. Our study indicates that PAH is part of an overall enzymatic and regulatory mechanism supplying NADPH required for lipogenesis in M. alpina.

ACS Style

Hongchao Wang; Chunmei Wang; Weiwei Yuan; Haiqin Chen; Wenwei Lu; Hao Zhang; Yong Q. Chen; Jianxin Zhao; Wei Chen. The role of phenylalanine hydroxylase in lipogenesis in the oleaginous fungus Mortierella alpina. Microbiology 2021, 167, 001062 .

AMA Style

Hongchao Wang, Chunmei Wang, Weiwei Yuan, Haiqin Chen, Wenwei Lu, Hao Zhang, Yong Q. Chen, Jianxin Zhao, Wei Chen. The role of phenylalanine hydroxylase in lipogenesis in the oleaginous fungus Mortierella alpina. Microbiology. 2021; 167 (8):001062.

Chicago/Turabian Style

Hongchao Wang; Chunmei Wang; Weiwei Yuan; Haiqin Chen; Wenwei Lu; Hao Zhang; Yong Q. Chen; Jianxin Zhao; Wei Chen. 2021. "The role of phenylalanine hydroxylase in lipogenesis in the oleaginous fungus Mortierella alpina." Microbiology 167, no. 8: 001062.

Journal article
Published: 11 August 2021 in Microorganisms
Reads 0
Downloads 0

Bifidobacterium longum is one of the most widely distributed and abundant Bifidobacterium in the human intestine, and has been proven to have a variety of physiological functions. In this study, 80 strains of B. longum isolated from human subjects were classified into subspecies by ANI and phylogenetic analyses, and the functional genes were compared. The results showed that there were significant differences in carbohydrate metabolism between the two subspecies, which determined their preference for human milk oligosaccharides or plant-derived carbohydrates. The predicted exopolysaccharide (EPS) gene clusters had large variability within species but without difference at the subspecies level. Four subtype CRISPR-Cas systems presented in B. longum, while the subtypes I-U and II-C only existed in B. longum subsp. longum. The bacteriocin operons in B. longum subsp. infantis were more widely distributed compared with B. longum subsp. longum. In conclusion, this study revealed the similarities and differences between B. longum subsp. infantis and B. longum subsp. longum, which could provide a theoretical basis for further exploring the probiotic characteristics of B. longum.

ACS Style

Mingjie Li; Xingya Zhou; Catherine Stanton; R. Paul Ross; Jianxin Zhao; Hao Zhang; Bo Yang; Wei Chen. Comparative Genomics Analyses Reveal the Differences between B. longum subsp. infantis and B. longum subsp. longum in Carbohydrate Utilisation, CRISPR-Cas Systems and Bacteriocin Operons. Microorganisms 2021, 9, 1713 .

AMA Style

Mingjie Li, Xingya Zhou, Catherine Stanton, R. Paul Ross, Jianxin Zhao, Hao Zhang, Bo Yang, Wei Chen. Comparative Genomics Analyses Reveal the Differences between B. longum subsp. infantis and B. longum subsp. longum in Carbohydrate Utilisation, CRISPR-Cas Systems and Bacteriocin Operons. Microorganisms. 2021; 9 (8):1713.

Chicago/Turabian Style

Mingjie Li; Xingya Zhou; Catherine Stanton; R. Paul Ross; Jianxin Zhao; Hao Zhang; Bo Yang; Wei Chen. 2021. "Comparative Genomics Analyses Reveal the Differences between B. longum subsp. infantis and B. longum subsp. longum in Carbohydrate Utilisation, CRISPR-Cas Systems and Bacteriocin Operons." Microorganisms 9, no. 8: 1713.

Journal article
Published: 25 July 2021 in Foods
Reads 0
Downloads 0

Increasing attention has been paid to the potential probiotic effects of Latilactobacillus sakei. To explore the genetic diversity of L. sakei, 14 strains isolated from different niches (feces, fermented kimchi, and meat products) and 54 published strains were compared and analyzed. The results showed that the average genome size and GC content of L.sakei were 1.98 Mb and 41.22%, respectively. Its core genome mainly encodes translation and transcription, amino acid synthesis, glucose metabolism, and defense functions. L.sakei has open pan-genomic characteristics, and its pan-gene curve shows an upward trend. The genetic diversity of L.sakei is mainly reflected in carbohydrate utilization, antibiotic tolerance, and immune/competition-related factors, such as clustering regular interval short palindromic repeat sequence (CRISPR)–Cas. The CRISPR system is mainly IIA type, and a few are IIC types. This work provides a basis for the study of this species.

ACS Style

Ying Chen; Nan Li; Shenxi Zhao; Chuan Zhang; Nanzhen Qiao; Hui Duan; Yue Xiao; Bowen Yan; Jianxin Zhao; Fengwei Tian; Qixiao Zhai; Leilei Yu; Wei Chen. Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches. Foods 2021, 10, 1717 .

AMA Style

Ying Chen, Nan Li, Shenxi Zhao, Chuan Zhang, Nanzhen Qiao, Hui Duan, Yue Xiao, Bowen Yan, Jianxin Zhao, Fengwei Tian, Qixiao Zhai, Leilei Yu, Wei Chen. Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches. Foods. 2021; 10 (8):1717.

Chicago/Turabian Style

Ying Chen; Nan Li; Shenxi Zhao; Chuan Zhang; Nanzhen Qiao; Hui Duan; Yue Xiao; Bowen Yan; Jianxin Zhao; Fengwei Tian; Qixiao Zhai; Leilei Yu; Wei Chen. 2021. "Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches." Foods 10, no. 8: 1717.

Review
Published: 20 July 2021 in Foods
Reads 0
Downloads 0

Accumulating evidence from animal studies supports the potential role of probiotics and prebiotics in alleviating neurodegenerative diseases. However, whether dietary supplementation with probiotics improves cognitive function in patients with Alzheimer’s disease (AD) or mild cognitive impairment (MCI) is unclear. We searched literature databases for relevant randomized control trials and compared the outcomes between control/placebo and intervention groups. The results of the included studies were meta-analyzed using a random-effects model, with standardized mean differences (SMDs) and 95% confidence intervals (CIs) calculated as summary statistics. We also performed a risk-of-bias assessment, sensitivity analysis and subgroup analysis. Among the 294 articles identified, eight articles involving 174 patients with AD and 446 with MCI were included in the qualitative synthesis and seven studies were meta-analyzed. Our analysis detected high between-group heterogeneity (SMD = 0.43, 95% CI −0.02–0.88, p< 0.0001, I2 = 86.4%) in cognitive function across the included studies. Subgroup analyses identified a significant effect of probiotics on cognitive function only in the studies involving people with MCI (I2 = 44%, p = 0.15 for heterogeneity, p = 0.0002 for overall effect). Our findings suggest that dietary supplementation with probiotics improves cognitive function, especially in people with MCI.

ACS Style

Guangsu Zhu; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. Probiotics for Mild Cognitive Impairment and Alzheimer’s Disease: A Systematic Review and Meta-Analysis. Foods 2021, 10, 1672 .

AMA Style

Guangsu Zhu, Jianxin Zhao, Hao Zhang, Wei Chen, Gang Wang. Probiotics for Mild Cognitive Impairment and Alzheimer’s Disease: A Systematic Review and Meta-Analysis. Foods. 2021; 10 (7):1672.

Chicago/Turabian Style

Guangsu Zhu; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. 2021. "Probiotics for Mild Cognitive Impairment and Alzheimer’s Disease: A Systematic Review and Meta-Analysis." Foods 10, no. 7: 1672.

Journal article
Published: 15 July 2021 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Bifidobacterium bifidum strains, an important component of probiotic foods, can form biofilms on abiotic surfaces, leading to increased self-resistance. However, little is known about the molecular mechanism of B. bifidum biofilm formation. A time series transcriptome sequencing and untargeted metabolomics analysis of both B. bifidum biofilm and planktonic cells was performed to identify key genes and metabolites involved in biofilm formation. Two hundred thirty-five nonredundant differentially expressed genes (DEGs) (including vanY, pstS, degP, groS, infC, groL, yajC, tadB and sigA) and 219 nonredundant differentially expressed metabolites (including L-threonine, L-cystine, L-tyrosine, ascorbic acid, niacinamide, butyric acid and sphinganine) were identified. Thirteen pathways were identified during the integration of both transcriptomics and metabolomics data, including ABC transporters; quorum sensing; two-component system; oxidative phosphorylation; cysteine and methionine metabolism; glutathione metabolism; glycine, serine and threonine metabolism; and valine, leucine and isoleucine biosynthesis. The DEGs that relate to the integration pathways included asd, atpB, degP, folC, ilvE, metC, pheA, pstS, pyrE, serB, ulaE, yajC and zwf. The differentially accumulated metabolites included L-cystine, L-serine, L-threonine, L-tyrosine, methylmalonate, monodehydroascorbate, nicotinamide, orthophosphate, spermine and tocopherol. These results indicate that quorum sensing, two-component system and amino acid metabolism are essential during B. bifidum biofilm formation.

ACS Style

Zongmin Liu; Lingzhi Li; Zhifeng Fang; Yuankun Lee; Jianxin Zhao; Hao Zhang; Wei Chen; Haitao Li; Wenwei Lu. Integration of Transcriptome and Metabolome Reveals the Genes and Metabolites Involved in Bifidobacterium bifidum Biofilm Formation. International Journal of Molecular Sciences 2021, 22, 7596 .

AMA Style

Zongmin Liu, Lingzhi Li, Zhifeng Fang, Yuankun Lee, Jianxin Zhao, Hao Zhang, Wei Chen, Haitao Li, Wenwei Lu. Integration of Transcriptome and Metabolome Reveals the Genes and Metabolites Involved in Bifidobacterium bifidum Biofilm Formation. International Journal of Molecular Sciences. 2021; 22 (14):7596.

Chicago/Turabian Style

Zongmin Liu; Lingzhi Li; Zhifeng Fang; Yuankun Lee; Jianxin Zhao; Hao Zhang; Wei Chen; Haitao Li; Wenwei Lu. 2021. "Integration of Transcriptome and Metabolome Reveals the Genes and Metabolites Involved in Bifidobacterium bifidum Biofilm Formation." International Journal of Molecular Sciences 22, no. 14: 7596.

Review article
Published: 08 July 2021 in Biotechnology Advances
Reads 0
Downloads 0

The oleaginous fungus Mortierella alpina has distinct advantages in long-chain PUFAs production, and it is the only source for dietary arachidonic acid (ARA) certificated by FDA and European Commission. This review provides an overall introduction to M. alpina, including its major research methods, key factors governing lipid biosynthesis, metabolic engineering and omics studies. Currently, the research interests in M. alpina focus on improving lipid yield and fatty acid desaturation degree by enhancing fatty acid precursors and the reducing power NADPH, and genetic manipulation on PUFAs synthetic pathways is carried to optimise fatty acid composition. Besides, multi-omics studies have been applied to elucidate the global regulatory mechanism of lipogenesis in M. alpina. However, research challenges towards achieving a lipid cell factory lie in strain breeding and cost control due to the coenocytic mycelium, long fermentation period and insufficient conversion rate from carbon to lipid. We also proposed future research goals based on a multilevel regulating strategy: obtaining ideal chassis by directional evolution and high-throughput screening; rewiring central carbon metabolism and inhibiting competitive pathways by multi-gene manipulation system to enhance carbon to lipid conversion rate; optimisation of protein function based on post-translational modification; application of dynamic fermentation strategies suitable for different fermentation phases. By reviewing the comprehensive research progress of this oleaginous fungus, we aim to further comprehend the fungal lipid metabolism and provide reference information and guidelines for the exploration of microbial oils from the perspectives of fundamental research to industrial application.

ACS Style

Lulu Chang; Hengqian Lu; Haiqin Chen; Xin Tang; Jianxin Zhao; Hao Zhang; Yong Q. Chen; Wei Chen. Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects. Biotechnology Advances 2021, 107794 .

AMA Style

Lulu Chang, Hengqian Lu, Haiqin Chen, Xin Tang, Jianxin Zhao, Hao Zhang, Yong Q. Chen, Wei Chen. Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects. Biotechnology Advances. 2021; ():107794.

Chicago/Turabian Style

Lulu Chang; Hengqian Lu; Haiqin Chen; Xin Tang; Jianxin Zhao; Hao Zhang; Yong Q. Chen; Wei Chen. 2021. "Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects." Biotechnology Advances , no. : 107794.

Journal article
Published: 01 July 2021 in Journal of Dairy Science
Reads 0
Downloads 0
ACS Style

Shenyan Ma; Jiang Cao; Ruolan Liliu; Nan Li; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. Effects of Bacillus coagulans as an adjunct starter culture on yogurt quality and storage. Journal of Dairy Science 2021, 104, 7466 -7479.

AMA Style

Shenyan Ma, Jiang Cao, Ruolan Liliu, Nan Li, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai. Effects of Bacillus coagulans as an adjunct starter culture on yogurt quality and storage. Journal of Dairy Science. 2021; 104 (7):7466-7479.

Chicago/Turabian Style

Shenyan Ma; Jiang Cao; Ruolan Liliu; Nan Li; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. 2021. "Effects of Bacillus coagulans as an adjunct starter culture on yogurt quality and storage." Journal of Dairy Science 104, no. 7: 7466-7479.

Paper
Published: 29 June 2021 in Food & Function
Reads 0
Downloads 0

The role of supplementation with different Bacteroides fragilis (B. fragilis) strains in alleviating ulcerative colitis (UC) is unclear due to the controversial results from animal experiments.

ACS Style

Chen Wang; Sijia Li; Kan Hong; Leilei Yu; Fengwei Tian; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. The roles of different Bacteroides fragilis strains in protecting against DSS-induced ulcerative colitis and related functional genes. Food & Function 2021, 1 .

AMA Style

Chen Wang, Sijia Li, Kan Hong, Leilei Yu, Fengwei Tian, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai. The roles of different Bacteroides fragilis strains in protecting against DSS-induced ulcerative colitis and related functional genes. Food & Function. 2021; ():1.

Chicago/Turabian Style

Chen Wang; Sijia Li; Kan Hong; Leilei Yu; Fengwei Tian; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. 2021. "The roles of different Bacteroides fragilis strains in protecting against DSS-induced ulcerative colitis and related functional genes." Food & Function , no. : 1.

Article
Published: 29 June 2021 in mSystems
Reads 0
Downloads 0

Lactobacilli are inherent microorganisms in the human gut and are widely used in the food processing industries due to their probiotic properties. Prophages were reportedly hidden in numerous Lactobacillus genomes and can potentially contaminate entire batches of fermentation or modulate the intestinal microecology once they are released.

ACS Style

Zhangming Pei; Faizan Ahmed Sadiq; Xiao Han; Jianxin Zhao; Hao Zhang; R. Paul Ross; Wenwei Lu; Wei Chen. Comprehensive Scanning of Prophages in Lactobacillus : Distribution, Diversity, Antibiotic Resistance Genes, and Linkages with CRISPR-Cas Systems. mSystems 2021, 6, 1 .

AMA Style

Zhangming Pei, Faizan Ahmed Sadiq, Xiao Han, Jianxin Zhao, Hao Zhang, R. Paul Ross, Wenwei Lu, Wei Chen. Comprehensive Scanning of Prophages in Lactobacillus : Distribution, Diversity, Antibiotic Resistance Genes, and Linkages with CRISPR-Cas Systems. mSystems. 2021; 6 (3):1.

Chicago/Turabian Style

Zhangming Pei; Faizan Ahmed Sadiq; Xiao Han; Jianxin Zhao; Hao Zhang; R. Paul Ross; Wenwei Lu; Wei Chen. 2021. "Comprehensive Scanning of Prophages in Lactobacillus : Distribution, Diversity, Antibiotic Resistance Genes, and Linkages with CRISPR-Cas Systems." mSystems 6, no. 3: 1.

Research article
Published: 22 June 2021 in Journal of Agricultural and Food Chemistry
Reads 0
Downloads 0

This research assessed the anti-inflammatory and hepatoprotective properties of inosine and the associated mechanism. Inosine pretreatment significantly reduced the secretion of several inflammatory factors and serum alanine transaminase (ALT) and aspartate amino transferase (AST) levels in a dose-dependent manner compared with the lipopolysaccharide (LPS) group. In LPS-treated mice, inosine pretreatment significantly reduced the ALT and malondialdehyde (MDA) concentration and significantly elevated the antioxidant enzyme activity. Furthermore, inosine pretreatment significantly altered the relative abundance of the genera, Bifidobacterium, Lachnospiraceae UCG-006, and Muribaculum. Correlation analysis showed that Bifidobacterium and Lachnospiraceae UCG-006 were positively related to the cecal short-chain fatty acids but negatively related to the serum IL-6 and hepatic AST and ALT levels. Notably, inosine pretreatment significantly modulated the hepatic TLR4, MYD88, NF-κB, iNOS, COX2, AMPK, Nfr2, and IκB-α expression. These results suggested that inosine pretreatment alters the intestinal microbiota structure and improves LPS-induced acute liver damage and inflammation through modulating the TLR4/NF-κB signaling pathway.

ACS Style

Weiling Guo; Qunran Xiang; Bingyong Mao; Xin Tang; Shumao Cui; Xiangfei Li; Jianxin Zhao; Hao Zhang; Wei Chen. Protective Effects of Microbiome-Derived Inosine on Lipopolysaccharide-Induced Acute Liver Damage and Inflammation in Mice via Mediating the TLR4/NF-κB Pathway. Journal of Agricultural and Food Chemistry 2021, 69, 7619 -7628.

AMA Style

Weiling Guo, Qunran Xiang, Bingyong Mao, Xin Tang, Shumao Cui, Xiangfei Li, Jianxin Zhao, Hao Zhang, Wei Chen. Protective Effects of Microbiome-Derived Inosine on Lipopolysaccharide-Induced Acute Liver Damage and Inflammation in Mice via Mediating the TLR4/NF-κB Pathway. Journal of Agricultural and Food Chemistry. 2021; 69 (27):7619-7628.

Chicago/Turabian Style

Weiling Guo; Qunran Xiang; Bingyong Mao; Xin Tang; Shumao Cui; Xiangfei Li; Jianxin Zhao; Hao Zhang; Wei Chen. 2021. "Protective Effects of Microbiome-Derived Inosine on Lipopolysaccharide-Induced Acute Liver Damage and Inflammation in Mice via Mediating the TLR4/NF-κB Pathway." Journal of Agricultural and Food Chemistry 69, no. 27: 7619-7628.

Journal article
Published: 11 June 2021 in Nutrients
Reads 0
Downloads 0

Psoriasis is an immune-mediated systemic disease that may be treated with probiotics. In this study, probiotic strains that could or could not decrease interleukin (IL)-17 levels were applied to imiquimod (IMQ)-induced psoriasis-like mice via oral administration. Bifidobacteriumadolescentis CCFM667, B. breve CCFM1078, Lacticaseibacillusparacasei CCFM1074, and Limosilactobacillus reuteri CCFM1132 ameliorated psoriasis-like pathological characteristics and suppressed the release of IL-23/T helper cell 17 (Th17) axis-related inflammatory cytokines, whereas B. animalis CCFM1148, L. paracasei CCFM1147, and L. reuteri CCFM1040 neither alleviated the pathological characteristics nor reduced the levels of inflammatory cytokines. All effective strains increased the contents of short-chain fatty acids, which were negatively correlated with the levels of inflammatory cytokines. By performing 16S rRNA gene sequencing, the diversity of gut microbiota in psoriasis-like mice was found to decrease, but all effective strains made some specific changes to the composition of gut microbiota compared to the ineffective strains. Furthermore, except for B. breve CCFM1078, all other effective strains decreased the abundance of the family Rikenellaceae, which was positively correlated with psoriasis-like pathological characteristics and was negatively correlated with propionate levels. These findings demonstrated effects of strain-specificity, and how probiotics ameliorated psoriasis and provide new possibilities for the treatment of psoriasis.

ACS Style

Wenwei Lu; Yadan Deng; Zhifeng Fang; Qixiao Zhai; Shumao Cui; Jianxin Zhao; Wei Chen; Hao Zhang. Potential Role of Probiotics in Ameliorating Psoriasis by Modulating Gut Microbiota in Imiquimod-Induced Psoriasis-Like Mice. Nutrients 2021, 13, 2010 .

AMA Style

Wenwei Lu, Yadan Deng, Zhifeng Fang, Qixiao Zhai, Shumao Cui, Jianxin Zhao, Wei Chen, Hao Zhang. Potential Role of Probiotics in Ameliorating Psoriasis by Modulating Gut Microbiota in Imiquimod-Induced Psoriasis-Like Mice. Nutrients. 2021; 13 (6):2010.

Chicago/Turabian Style

Wenwei Lu; Yadan Deng; Zhifeng Fang; Qixiao Zhai; Shumao Cui; Jianxin Zhao; Wei Chen; Hao Zhang. 2021. "Potential Role of Probiotics in Ameliorating Psoriasis by Modulating Gut Microbiota in Imiquimod-Induced Psoriasis-Like Mice." Nutrients 13, no. 6: 2010.

Journal article
Published: 11 June 2021 in Foods
Reads 0
Downloads 0

Lactobacillus ruminis can stimulate the immune response in vitro, but previous studies were only carried out in vitro and the anti-inflammatory effects of L. ruminis needs more in vivo evidences. In this study, the immune regulation and potential mechanisms of L. ruminis was investigated in DSS-induced colitis mice. L. ruminis FXJWS27L3 and L. ruminis FXJSW17L1 relieved the symptoms of colitis, including inhibition of colon shortening and colon tissue damage. L. ruminis FXJWS27L3 significantly reduced the pro-inflammatory cytokines IL-1β, TNF-α, and IL-17, while L. ruminis FXJSW17L1 significantly increased short chain fatty acids in mice feces. Moreover, L. ruminis FXJWS27L3 and L. ruminis FXJSW17L1 treatments significantly increased the gut microbiota diversity and balance the intestine microbiota profiles, which improved the imbalance of intestine microbiota composition to a certain extent. The results showed that L. ruminis can alleviate DSS-induced colitis, which possibly was related to promoting the expression of pro-inflammatory cytokines, up-regulating SCFAs and restoring the imbalance of gut microbiota.

ACS Style

Bo Yang; Mingjie Li; Shuo Wang; R. Ross; Catherine Stanton; Jianxin Zhao; Hao Zhang; Wei Chen. Lactobacillus ruminis Alleviates DSS-Induced Colitis by Inflammatory Cytokines and Gut Microbiota Modulation. Foods 2021, 10, 1349 .

AMA Style

Bo Yang, Mingjie Li, Shuo Wang, R. Ross, Catherine Stanton, Jianxin Zhao, Hao Zhang, Wei Chen. Lactobacillus ruminis Alleviates DSS-Induced Colitis by Inflammatory Cytokines and Gut Microbiota Modulation. Foods. 2021; 10 (6):1349.

Chicago/Turabian Style

Bo Yang; Mingjie Li; Shuo Wang; R. Ross; Catherine Stanton; Jianxin Zhao; Hao Zhang; Wei Chen. 2021. "Lactobacillus ruminis Alleviates DSS-Induced Colitis by Inflammatory Cytokines and Gut Microbiota Modulation." Foods 10, no. 6: 1349.

Paper
Published: 02 June 2021 in Food & Function
Reads 0
Downloads 0

L. rhamnosus R31, L. rhamnosus R28-1 and L. reuteri L20M3 relieved hyperuricaemia in mouse model by promoting SCFA production.

ACS Style

Caixin Ni; Xin Li; Linlin Wang; Xiu Li; Jianxin Zhao; Hao Zhang; Gang Wang; Wei Chen. Lactic acid bacteria strains relieve hyperuricaemia by suppressing xanthine oxidase activity via a short-chain fatty acid-dependent mechanism. Food & Function 2021, 1 .

AMA Style

Caixin Ni, Xin Li, Linlin Wang, Xiu Li, Jianxin Zhao, Hao Zhang, Gang Wang, Wei Chen. Lactic acid bacteria strains relieve hyperuricaemia by suppressing xanthine oxidase activity via a short-chain fatty acid-dependent mechanism. Food & Function. 2021; ():1.

Chicago/Turabian Style

Caixin Ni; Xin Li; Linlin Wang; Xiu Li; Jianxin Zhao; Hao Zhang; Gang Wang; Wei Chen. 2021. "Lactic acid bacteria strains relieve hyperuricaemia by suppressing xanthine oxidase activity via a short-chain fatty acid-dependent mechanism." Food & Function , no. : 1.

Journal article
Published: 28 May 2021 in Microorganisms
Reads 0
Downloads 0

We adopted a bioinformatics-based technique to identify strain-specific markers, which were then used to quantify the abundances of three distinct B. longum sup. longum strains in fecal samples of humans and mice. A pangenome analysis of 205 B. longum sup. longum genomes revealed the accumulation of considerable strain-specific genes within this species; specifically, 28.7% of the total identified genes were strain-specific. We identified 32, 14, and 49 genes specific to B. longum sup. longum RG4-1, B. longum sup. longum M1-20-R01-3, and B. longum sup. longum FGSZY6M4, respectively. After performing an in silico validation of these strain-specific markers using a nucleotide BLAST against both the B. longum sup. longum genome database and an NR/NT database, RG4-1_01874 (1331 bp), M1-20-R01-3_00324 (1745 bp), and FGSZY6M4_01477 (1691 bp) were chosen as target genes for strain-specific quantification. The specificities of the qPCR primers were validated against 47 non-target microorganisms and fecal baseline microbiota to ensure that they produced no PCR amplification products. The performance of the qPCR primer-based analysis was further assessed using fecal samples. After oral administration, the target B. longum strains appeared to efficiently colonize both the human and mouse guts, with average population levels of >108 CFU/g feces. The bioinformatics pipeline proposed here can be applied to the quantification of various bacterial species.

ACS Style

Yue Xiao; Chen Wang; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. Quantitative Detection of Bifidobacterium longum Strains in Feces Using Strain-Specific Primers. Microorganisms 2021, 9, 1159 .

AMA Style

Yue Xiao, Chen Wang, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai. Quantitative Detection of Bifidobacterium longum Strains in Feces Using Strain-Specific Primers. Microorganisms. 2021; 9 (6):1159.

Chicago/Turabian Style

Yue Xiao; Chen Wang; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. 2021. "Quantitative Detection of Bifidobacterium longum Strains in Feces Using Strain-Specific Primers." Microorganisms 9, no. 6: 1159.

Review
Published: 26 May 2021 in Journal of Functional Foods
Reads 0
Downloads 0

Recently, a bidirectional relationship between dietary lead (Pb) exposure and gut changes has been identified. Pb causes gut dysbacteriosis and destroys gut physiological homeostasis, causing a series of oxidative stress, immune-inflammatory reactions and increased permeability of the intestinal barrier. Pb-induced gut dysbiosis and physiological disorders are related to impairments in nutrient absorption, essential element homeostasis, lipid metabolism, and nervous system, which can in turn influence Pb transport and metabolism. Dietary supplements such as probiotics, prebiotics, minerals, and antioxidants could be a potential strategy to alleviate Pb toxicity. They can protect the gut barrier, promote Pb discharge, and regulate oxidative stress and essential metal levels, which likely depend on the gut microbiota. Thus, dietary supplements, especially probiotics and prebiotics, are a new and safe strategy to prevent and alleviate Pb poisoning in the future.

ACS Style

Leilei Yu; Yaqi Yu; Yue Xiao; Fengwei Tian; Arjan Narbad; Qixiao Zhai; Wei Chen. Lead-induced gut injuries and the dietary protective strategies: A review. Journal of Functional Foods 2021, 83, 104528 .

AMA Style

Leilei Yu, Yaqi Yu, Yue Xiao, Fengwei Tian, Arjan Narbad, Qixiao Zhai, Wei Chen. Lead-induced gut injuries and the dietary protective strategies: A review. Journal of Functional Foods. 2021; 83 ():104528.

Chicago/Turabian Style

Leilei Yu; Yaqi Yu; Yue Xiao; Fengwei Tian; Arjan Narbad; Qixiao Zhai; Wei Chen. 2021. "Lead-induced gut injuries and the dietary protective strategies: A review." Journal of Functional Foods 83, no. : 104528.

Journal article
Published: 25 May 2021 in Nutrients
Reads 0
Downloads 0

The decrease in ovarian hormone secretion that occurs during menopause results in an increase in body weight and adipose tissue mass. Probiotics and soy isoflavones (SIFs) could affect the gut microbiota and exert anti-obesity effects. The objective of this study was to investigate the effects of probiotics and a diet containing SIF (SIF diet) on ovariectomized mice with menopausal obesity, including the gut microbiome. The results demonstrate that Bifidobacterium longum 15M1 can reverse menopausal obesity, whilst the combination of Lactobacillus plantarum 30M5 and a SIF diet was more effective in alleviating menopausal lipid metabolism disorder than either components alone. Probiotics and SIFs play different anti-obesity roles in menopausal mice. Furthermore, 30M5 alters the metabolites of the gut microbiota that increase the circulating estrogen level, upregulates the expression of estrogen receptor α in abdominal adipose tissue and improves the production of short-chain fatty acids (SCFAs). A SIF diet can significantly alter the structure of the fecal bacterial community and enrich the pathways related to SCFAs production. Moreover, 30M5 and a SIF diet acted synergistically to effectively resolve abnormal serum lipid levels in ovariectomized mice, and these effects appear to be associated with regulation of the diversity and structure of the intestinal microbiota to enhance SCFAs production and promote estrogen circulation.

ACS Style

Qian Chen; Botao Wang; Shunhe Wang; Xin Qian; Xiu Li; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. Modulation of the Gut Microbiota Structure with Probiotics and Isoflavone Alleviates Metabolic Disorder in Ovariectomized Mice. Nutrients 2021, 13, 1793 .

AMA Style

Qian Chen, Botao Wang, Shunhe Wang, Xin Qian, Xiu Li, Jianxin Zhao, Hao Zhang, Wei Chen, Gang Wang. Modulation of the Gut Microbiota Structure with Probiotics and Isoflavone Alleviates Metabolic Disorder in Ovariectomized Mice. Nutrients. 2021; 13 (6):1793.

Chicago/Turabian Style

Qian Chen; Botao Wang; Shunhe Wang; Xin Qian; Xiu Li; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. 2021. "Modulation of the Gut Microbiota Structure with Probiotics and Isoflavone Alleviates Metabolic Disorder in Ovariectomized Mice." Nutrients 13, no. 6: 1793.

Paper
Published: 19 May 2021 in Food & Function
Reads 0
Downloads 0

B. bifidum had intraspecies differences in relieving constipation. It can promote intestinal peristalsis by gastrointestinal active peptides. Specially, FXJCJ32M2 may promote the expression of AQP8 and VIPR1 through gut microbiota modulation.

ACS Style

Mao Chai; Linlin Wang; Xinping Li; Jianxin Zhao; Hao Zhang; Gang Wang; Wei Chen. Different Bifidobacterium bifidum strains change the intestinal flora composition of mice via different mechanisms to alleviate loperamide-induced constipation. Food & Function 2021, 1 .

AMA Style

Mao Chai, Linlin Wang, Xinping Li, Jianxin Zhao, Hao Zhang, Gang Wang, Wei Chen. Different Bifidobacterium bifidum strains change the intestinal flora composition of mice via different mechanisms to alleviate loperamide-induced constipation. Food & Function. 2021; ():1.

Chicago/Turabian Style

Mao Chai; Linlin Wang; Xinping Li; Jianxin Zhao; Hao Zhang; Gang Wang; Wei Chen. 2021. "Different Bifidobacterium bifidum strains change the intestinal flora composition of mice via different mechanisms to alleviate loperamide-induced constipation." Food & Function , no. : 1.

Paper
Published: 18 May 2021 in Food & Function
Reads 0
Downloads 0

Enterotoxigenic Escherichia coli (ETEC) is the main bacterial cause of diarrhea among children in developing countries and of traveler's diarrhea.

ACS Style

Bo Yang; Zheng Huang; Zhujun He; Yue Yue; Yonghua Zhou; R. Paul Ross; Catherine Stanton; Hao Zhang; Jianxin Zhao; Wei Chen. Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli. Food & Function 2021, 12, 7271 -7282.

AMA Style

Bo Yang, Zheng Huang, Zhujun He, Yue Yue, Yonghua Zhou, R. Paul Ross, Catherine Stanton, Hao Zhang, Jianxin Zhao, Wei Chen. Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli. Food & Function. 2021; 12 (16):7271-7282.

Chicago/Turabian Style

Bo Yang; Zheng Huang; Zhujun He; Yue Yue; Yonghua Zhou; R. Paul Ross; Catherine Stanton; Hao Zhang; Jianxin Zhao; Wei Chen. 2021. "Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli." Food & Function 12, no. 16: 7271-7282.

Paper
Published: 18 May 2021 in Food & Function
Reads 0
Downloads 0

A total of 51 Lactobacillus species were detected in 200 human fecal samples, and a new optimized medium called LFMATA (containing arabinose, vancomycin, gentamicin and streptomycin) for the isolation of L. fermentum strains was developed.

ACS Style

Yan Zhao; Leilei Yu; Fengwei Tian; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. An optimized culture medium to isolate Lactobacillus fermentum strains from the human intestinal tract. Food & Function 2021, 1 .

AMA Style

Yan Zhao, Leilei Yu, Fengwei Tian, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai. An optimized culture medium to isolate Lactobacillus fermentum strains from the human intestinal tract. Food & Function. 2021; ():1.

Chicago/Turabian Style

Yan Zhao; Leilei Yu; Fengwei Tian; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. 2021. "An optimized culture medium to isolate Lactobacillus fermentum strains from the human intestinal tract." Food & Function , no. : 1.

Original article
Published: 17 May 2021 in International Journal of Food Science & Technology
Reads 0
Downloads 0

Lactobacillus plantarum proliferates inefficiently in milk, mainly because of its lack of cell envelope proteases and its inability to hydrolyse proteins in milk. Our previous study showed that this strain could grow well in milk with the addition of oat and malt extracts. To investigate the usage and preference for polypeptides and oligopeptides for this strain, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), o-phthaldialdehyde (OPA), high-performance liquid chromatography (HPLC), plate counting and other methods were used in this study. The results showed that proteins in fermented milk cannot be absorbed and utilised by L. plantarum, whereas polypeptides and oligopeptides provide available nitrogen sources for their growth. Short-chain peptides were more conducive to absorption and utilisation than long-chain peptides. In particular, peptides with molecular weights in the range of 200–1400 Da in the oat extract and 100–700 Da in the malt extract were preferentially absorbed and utilised.

ACS Style

Yuanzhi Jiang; Yangyang Shi; Rui Li; Feng Hang; Jianxin Zhao; Hao Zhang; Wei Chen. The peptides in oat and malt extracts that are preferentially absorbed by Lactobacillus plantarum and stimulates its proliferation in milk. International Journal of Food Science & Technology 2021, 1 .

AMA Style

Yuanzhi Jiang, Yangyang Shi, Rui Li, Feng Hang, Jianxin Zhao, Hao Zhang, Wei Chen. The peptides in oat and malt extracts that are preferentially absorbed by Lactobacillus plantarum and stimulates its proliferation in milk. International Journal of Food Science & Technology. 2021; ():1.

Chicago/Turabian Style

Yuanzhi Jiang; Yangyang Shi; Rui Li; Feng Hang; Jianxin Zhao; Hao Zhang; Wei Chen. 2021. "The peptides in oat and malt extracts that are preferentially absorbed by Lactobacillus plantarum and stimulates its proliferation in milk." International Journal of Food Science & Technology , no. : 1.