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Mechanical properties of tumor cytoskeleton are extremely vital for any phases of cancer, especially in tumor invasion and metastasis. However, in current category of anticancer drugs, the cytoskeleton-targeting drugs are limited and its role in tumor progression is unclear. Here, we present the mechanical characteristics of tumor stiffness are tightly regulated by cancer cytoskeleton including actin filaments and microtubule during tumor initiation, growth and metastasis, and review the natural drugs that target cancer cytoskeleton. We define cytoskeleton dynamics as target mechanisms for anticancer drug, and summary the plant, microbial and marine sources of natural products. Furthermore, the material approaches to active cancer mechanics are supplied in this review. We aim to promote the development of anticancer drugs that target tumor mechanics by using those material approaches in future and find its pharmacological application.
Xiaoye Liu; Hongwei Xu; Yuting Feng; Xiaolin Hou. Development of Anticancer Drugs: From Mechanical Properties of Tumor Stiffness to Cytoskeleton-Targeting Natural Products. 2021, 1 .
AMA StyleXiaoye Liu, Hongwei Xu, Yuting Feng, Xiaolin Hou. Development of Anticancer Drugs: From Mechanical Properties of Tumor Stiffness to Cytoskeleton-Targeting Natural Products. . 2021; ():1.
Chicago/Turabian StyleXiaoye Liu; Hongwei Xu; Yuting Feng; Xiaolin Hou. 2021. "Development of Anticancer Drugs: From Mechanical Properties of Tumor Stiffness to Cytoskeleton-Targeting Natural Products." , no. : 1.
Antibiotic therapy and host cells frequently fail to eliminate invasive bacterial pathogens due to the emergence of antibiotic resistance, resulting in the relapse and recurrence of infections. Bacteria evolve various strategies to persist and survive in epithelial cells, a front‐line barrier of host tissues counteracting invasion; however, it remains unclear how bacteria hijack cellular responses to promote cytoplasmic survival under antibiotic therapy. Here, it is demonstrated that extracellular bacteria show invasive behavior and survive in epithelial cells in both in vivo and in vitro models, to increase antibiotic tolerance. In turn, sublethal levels of antibiotics increase bacterial invasion through promoting the production of bacterial virulence factors. Furthermore, antibiotic treatments interrupt lysosomal acidification in autophagy due to the internalized bacteria, using Bacillus cereus and ciprofloxacin as a model. In addition, it is found that sublethal levels of ciprofloxacin cause mitochondrial dysfunction and reactive oxygen species (ROS) accumulation to impair lysosomal vascular tape ATPase (V‐ATPase) to further promote bacterial persistence. Collectively, these results highlight the potential of host cells mediated antibiotic tolerance, which markedly compromises antibiotic efficacy and worsens the outcomes of infection.
Xiaoye Liu; Fei Liu; Shuangyang Ding; Jianzhong Shen; Kui Zhu. Sublethal Levels of Antibiotics Promote Bacterial Persistence in Epithelial Cells. Advanced Science 2020, 7, 1 .
AMA StyleXiaoye Liu, Fei Liu, Shuangyang Ding, Jianzhong Shen, Kui Zhu. Sublethal Levels of Antibiotics Promote Bacterial Persistence in Epithelial Cells. Advanced Science. 2020; 7 (18):1.
Chicago/Turabian StyleXiaoye Liu; Fei Liu; Shuangyang Ding; Jianzhong Shen; Kui Zhu. 2020. "Sublethal Levels of Antibiotics Promote Bacterial Persistence in Epithelial Cells." Advanced Science 7, no. 18: 1.
Bacillus cereus is a common and ubiquitous foodborne pathogen with an increasing prevalence rate in dairy products in China. High and unmet demands for such products, particularly milk, raise the risk of B. cereus associated contamination. The presence of B. cereus and its virulence factors in dairy products may cause food poisoning and other illnesses. Thus, this review first summarizes the epidemiological characteristics and analytical assays of B. cereus from dairy products in China, providing insights into the implementation of intervention strategies. In addition, the recent achievements on the cytotoxicity and mechanisms of B. cereus are also presented to shed light on the therapeutic options for B. cereus associated infections.
Xiao-Ye Liu; Qiao Hu; Fei Xu; Shuang-Yang Ding; Kui Zhu. Characterization of Bacillus cereus in Dairy Products in China. Toxins 2020, 12, 454 .
AMA StyleXiao-Ye Liu, Qiao Hu, Fei Xu, Shuang-Yang Ding, Kui Zhu. Characterization of Bacillus cereus in Dairy Products in China. Toxins. 2020; 12 (7):454.
Chicago/Turabian StyleXiao-Ye Liu; Qiao Hu; Fei Xu; Shuang-Yang Ding; Kui Zhu. 2020. "Characterization of Bacillus cereus in Dairy Products in China." Toxins 12, no. 7: 454.
Migration of neutrophils across endothelial barriers to capture and eliminate bacteria is served as the first line of innate immunity. Bacterial virulence factors damage endothelium to produce inflammatory cytokines interacts with neutrophils. However, the mechanisms that behind endothelial-neutrophil interaction impact on the bactericidal activity remain unclear. Therefore, we aimed to find the target proteins on endothelial cells that triggered the bactericidal activity of transendothelial neutrophils. Herein, we built the infected models on rats and endothelial-neutrophil co-cultural system (Transwell) and discovered that endothelial-derived IL-1α promoted the survival of rats under Escherichia coli infection and enhanced the bactericidal activity of transendothelial neutrophils in vivo and in vitro. Results further showed that IL-1α was inhibited by lipopolysaccharide (LPS) in the endothelial-neutrophil interaction. We found that LPS mainly damaged cell membrane and induced cell necrosis to interrupt neutrophil migration from endothelial barrier. Thus, we used the isobaric tags for relative and absolute quantification (iTRAQ) method to identify different proteins of endothelial cells. Results showed that IL-1α targeted cellular plasma membrane, endoplasmic reticulum and mitochondrial envelope and triggered eleven common proteins to persistently regulate. During the early phase, IL-1α triggered the upregulation of cell adhesion molecules (CAMs) to promote neutrophil adhesion, while oxidative phosphorylation was involved in long time regulation to induce transmigration of neutrophils against bacteria. Our results highlight the critical mechanism of endothelial-derived IL-1α on promoting bactericidal activity of transendothelial neutrophils and the findings of IL-1α triggered proteins provide the potentially important targets on the regulation of innate immunity.
Xiaoye Liu; Hui Zhang; Shangwen He; Xiang Mu; Ge Hu; Hong Dong. Endothelial-Derived Interleukin-1α Activates Innate Immunity by Promoting the Bactericidal Activity of Transendothelial Neutrophils. Frontiers in Cell and Developmental Biology 2020, 8, 590 .
AMA StyleXiaoye Liu, Hui Zhang, Shangwen He, Xiang Mu, Ge Hu, Hong Dong. Endothelial-Derived Interleukin-1α Activates Innate Immunity by Promoting the Bactericidal Activity of Transendothelial Neutrophils. Frontiers in Cell and Developmental Biology. 2020; 8 ():590.
Chicago/Turabian StyleXiaoye Liu; Hui Zhang; Shangwen He; Xiang Mu; Ge Hu; Hong Dong. 2020. "Endothelial-Derived Interleukin-1α Activates Innate Immunity by Promoting the Bactericidal Activity of Transendothelial Neutrophils." Frontiers in Cell and Developmental Biology 8, no. : 590.
Gut microbiota serves as a critical indicator for gut health during treatment of pathogenic bacterial infection. Both Pulsatilla Decoction (abbreviated to PD, a traditional Chinese medicine compound) and Levofloxacin Hydrochloride (LVX) were known to have therapeutic effects to intestinal infectious disease. However, the changes of gut microbiota after PD or LVX treatment remain unclear. Herein, this work aimed to investigate the changes of intestinal flora after PD or LVX therapy of Escherichia coli infection in rats. Results revealed that PD exhibited a valid therapeutic approach for E. coli infection via the intestinal protection, as well as the inhibited release of IL-8 and ICAM-1. Besides, PD was beneficial to rebuild the gut microbiota via restoring Bacteroidetes spp in the composition of the gut microbiota. Comparatively, LVX treatment promoted the infection and ravaged gut microbiota by significantly decreasing Bacteroidetes and increasing Firmicutes. These findings not only highlight the mechanism of Chinese herbal formula, but extend the application of PD as veterinary medicine, feed additive and pre-mixing agent for improving the production of animal derived foods.
Xiaoye Liu; Shangwen He; Qiuyue Li; Xiang Mu; Ge Hu; Hong Dong. Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection. Frontiers in Cellular and Infection Microbiology 2020, 10, 319 .
AMA StyleXiaoye Liu, Shangwen He, Qiuyue Li, Xiang Mu, Ge Hu, Hong Dong. Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection. Frontiers in Cellular and Infection Microbiology. 2020; 10 ():319.
Chicago/Turabian StyleXiaoye Liu; Shangwen He; Qiuyue Li; Xiang Mu; Ge Hu; Hong Dong. 2020. "Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection." Frontiers in Cellular and Infection Microbiology 10, no. : 319.