Handheld chlorophyll meters as Soil Plant Analysis Development (SPAD) have proven to be useful tools for rapid, no-destructive assessment of chlorophyll and nitrogen status in various crops. This method is used to diagnose the need of nitrogen fertilization to improve the efficiency of the agricultural system and to minimize nitrogen losses and deficiency. The objective of this study is to evaluate the effect of repeated conservative agriculture practices on the SPAD readings, leaves chlorophyll concentration and Nitrogen Nutrition Index (NNI) relationships in durum wheat under Mediterranean conditions. The experimental site is a part of a long-term-experiment established in 1994 and is still on-going where three tillage managements and three nitrogen fertilizer treatments were repeated in the same plots every year. We observed a linear relationship between the SPAD readings performed in the central and distal portion of the leaf (R² = 0.96). In fertilized durum wheat, we found all positive exponential relationships between SPAD readings, chlorophyll leaves concentration (R² = 0.85) and NNI (R² = 0.89). In the unfertilized treatment, the SPAD has a good attitude to estimate leaves chlorophyll concentration (R² = 0.74) and NNI (R² = 0.77) only in crop grow a soil with relative high content of soil organic matter and nitrogen availability, as observed in the no tilled plots. The results show that the SPAD can be used for a correct assessment of chlorophyll and nitrogen status in durum wheat but also to evaluate indirectly the content of soil organic matter and nitrogen availability during different growth stages of the crop cycle.

Proximal soil sensors are receiving strong attention from several disciplinary fields, and this has led to a rise in their availability in the market in the last two decades. The aim of this work was to validate agronomically a zone management delineation procedure from electromagnetic induction (EMI) maps applied to two different rainfed durum wheat fields. The k-means algorithm was applied based on the gap statistic index for the identification of the optimal number of management zones and their positions. Traditional statistical analysis was performed to detect significant differences in soil characteristics and crop response of each management zones. The procedure showed the presence of two management zones at both two sites under analysis, and it was agronomically validated by the significant difference in soil texture (+24.17%), bulk density (+6.46%), organic matter (+39.29%), organic carbon (+39.4%), total carbonates (+25.34%), total nitrogen (+30.14%), protein (+1.50%) and yield data (+1.07 t ha⁻¹). Moreover, six unmanned aerial vehicle (UAV) flight missions were performed to investigate the relationship between five vegetation indexes and the EMI maps. The results suggest performing the multispectral images acquisition during the flowering phenological stages to attribute the crop spatial variability to different soil proprieties.

Combining remote and proximal sensing in agriculture is essential to monitor crop spatial-temporal variability and to provide high-quality prescription maps for the precision agriculture applications. The study showed how different combinations of soil management (no tillage—NT vs. conventional tillage—CT) and nitrogen (N) fertilization levels (0.90 and 180 kg N ha⁻¹) can affect the durum wheat nutritional status and development through vegetation indices computation and proximal sensing tool application. Chlorophyll and N crop content were measured, in addition a proximal sensing tool and multispectral imagery equipped on unmanned aerial vehicle were used. The N input is the key driver for durum wheat development (4.5 ± 0.92 t ha⁻¹ on average), but when it was not provided the NT performed better than CT (2.51 ± 0.22 vs. 1.46 ± 0.28 t ha⁻¹ respectively) in terms of grain yield. This is due to the greater content of organic matter and N availability which characterizes the NT system. The near infrared (NIR) band-based vegetation indices can well detect the durum wheat nutritional status (R² = 0.70 on average). The showed results can provide an important contribution in the implementation of ago-environmental policies aimed at environmental impact of cereal-based-cropping systems reduction.