Anastasia Cooper

Dr. Anastasia Cooper

Kansas State University
orcid

Research Focus & Metrics

Academic Career Length

7Years (2020-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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13 January 2021 in Pest Management Science
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Journal Article
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KSKristopher Silver
Kristopher Silver
Anastasia CooperAnastasia Cooper
Anastasia Cooper
Kun Yan ZhuKun Yan Zhu
Kun Yan Zhu

1 October 2020 in Pest Management Science
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Journal Article
Closed access
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Anastasia CooperAnastasia Cooper
Anastasia Cooper
HSHuifang Song
Huifang Song
ZYZhitao Yu
Zhitao Yu
MBMarie Biondi
Marie Biondi
JBJun Bai
Jun Bai
Kun Yan ZhuKun Yan Zhu
Kun Yan Zhu

23 September 2020 in Insects
Verified
Journal Article
Open access
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Anastasia CooperAnastasia Cooper
Anastasia Cooper
HSHuifang Song
Huifang Song
XSXuekai Shi
Xuekai Shi
ZYZhitao Yu
Zhitao Yu
Marce LorenzenMarce Lorenzen
Marce Lorenzen
Kun Yan ZhuKun Yan Zhu
Kun Yan Zhu
Variable RNA interference (RNAi) efficiencies limit RNAi-based pest management strategies for many pests. Previous efforts to understand mechanisms contributing to low RNAi efficiency indicate that double-stranded RNA (dsRNA) is degraded in the European corn borer (ECB), Ostrinia nubilalis, due to nuclease activity. To investigate the contribution of dsRNA-degrading endonucleases (dsRNases) and lepidopteran-specific RNAi efficiency-related nucleases (REases) to dsRNA instability and low RNAi efficiency in ECB, five complementary DNAs putatively encoding four dsRNases (OndsRNase1, 2, 3, and 4) and one REase (OnREase) were sequenced. Characterization of these transcripts revealed that substrate specificity might vary among the four dsRNases due to different amino acid combinations in the substrate-binding sites. Gene expression analysis indicated that OndsRNase2 and OnREase were highly expressed in the larval gut, and OndsRNase1 showed the highest expression in hemolymph, especially in older developmental stages. Transcript level analysis after dsRNA exposure revealed that expression of OnREase rapidly increased upon dsRNA ingestion or injection, whereas OndsRNase4 expression only increased after long-term ingestion of dsRNA. While the biological function of these nucleases remains to be verified, our results suggest that OnREase and OndsRNase2, and OndsRNase1 and OndsRNase4 may be responsible for degradation of dsRNAs in the ECB gut and hemolymph, respectively, thereby contributing to low RNAi efficiency.

1 February 2021 in Journal of Insect Physiology
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Journal Article
Closed access
Anastasia CooperAnastasia Cooper
Anastasia Cooper
HSHuifang Song
Huifang Song
XSXuekai Shi
Xuekai Shi
ZYZhitao Yu
Zhitao Yu
Marce LorenzenMarce Lorenzen
Marce Lorenzen
Kun Yan ZhuKun Yan Zhu
Kun Yan Zhu

9 November 2023 in Insect Science
Verified
Journal Article
Closed access
Anastasia CooperAnastasia Cooper
Anastasia Cooper
SJSamuel B. Jameson
Samuel B. Jameson
VPVictoria Pickens
Victoria Pickens
COCameron Osborne
Cameron Osborne
EBElaine A. Backus
Elaine A. Backus
DMDana Mitzel
Dana Mitzel

19 April 2021 in Archives of Insect Biochemistry and Physiology
Verified
Journal Article
Closed access
PDF file
Anastasia CooperAnastasia Cooper
Anastasia Cooper
HSHuifang Song
Huifang Song
XSXuekai Shi
Xuekai Shi
ZYZhitao Yu
Zhitao Yu
YKYoung Ho Kim
Young Ho Kim
Kun Yan ZhuKun Yan Zhu
Kun Yan Zhu