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Born in Italy, May 25th, 1950. Doctor in Chemistry (1975), University of Bari, Italy. Contract Researcher 1976-1979, Contract Professor 1980-1984, University of Bari, Italy. Researcher at ENIRICERCHE (formerly ASSORENI), the Association of Companies in the ENI Industrial Group (National Petroleum Company - Holding) for Research Development and Planning in Agriculture (1982-1984). Associate Professor of Agricultural Chemistry 1985-1994, University of Bari, Italy. Full Professor of Soil and Agricultural Chemistry from November 1st, 1994, University of Basilicata, Department of Sciences. Coordinator of the International PhD Programme ‘Applied Biology & Environmental Safeguard’. Research topics: chemistry of natural substances in plants and soil; pharmaceuticals from plants and microorganisms; fate of xenobiotics in the environment.
Crude oil can undergo biotic and abiotic transformation processes in the environment. This article deals with the fate of an Italian crude oil under simulated solar irradiation to understand (i) the modification induced on its composition by artificial ageing and (ii) the transformations arising from different advanced oxidation processes (AOPs) applied as oil-polluted water remediation methods. The AOPs adopted were photocatalysis, sonolysis and, simultaneously, photocatalysis and sonolysis (sonophotocatalysis). Crude oil and its water-soluble fractions underwent analysis using GC-MS, liquid-state 1H-NMR, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), and fluorescence. The crude oil after light irradiation showed (i) significant modifications induced by the artificial ageing on its composition and (ii) the formation of potentially toxic substances. The treatment produced oil oxidation with a particular effect of double bonds oxygenation. Non-polar compounds present in the water-soluble oil fraction showed a strong presence of branched alkanes and a good amount of linear and aromatic alkanes. All remediation methods utilised generated an increase of C5 class and a decrease of C6–C9 types of compounds. The analysis of polar molecules elucidated that oxygenated compounds underwent a slight reduction after photocatalysis and a sharp decline after sonophotocatalytic degradation. Significant modifications did not occur by sonolysis.
Filomena Lelario; Giuliana Bianco; Sabino Bufo; Laura Scrano. Simulated Ageing of Crude Oil and Advanced Oxidation Processes for Water Remediation since Crude Oil Pollution. Catalysts 2021, 11, 954 .
AMA StyleFilomena Lelario, Giuliana Bianco, Sabino Bufo, Laura Scrano. Simulated Ageing of Crude Oil and Advanced Oxidation Processes for Water Remediation since Crude Oil Pollution. Catalysts. 2021; 11 (8):954.
Chicago/Turabian StyleFilomena Lelario; Giuliana Bianco; Sabino Bufo; Laura Scrano. 2021. "Simulated Ageing of Crude Oil and Advanced Oxidation Processes for Water Remediation since Crude Oil Pollution." Catalysts 11, no. 8: 954.
Diterpenoid alkaloids are natural compounds having complex structural features with many stereo-centres originating from the amination of natural tetracyclic diterpenes and produced primarily from plants in the Aconitum, Delphinium, Consolida genera. Corals, Xenia, Okinawan/Clavularia, Alcyonacea (soft corals) and marine sponges are rich sources of diterpenoids, despite the difficulty to access them and the lack of availability. Researchers have long been concerned with the potential beneficial or harmful effects of diterpenoid alkaloids due to their structural complexity, which accounts for their use as pharmaceuticals as well as their lousy reputation as toxic substances. Compounds belonging to this unique and fascinating family of natural products exhibit a broad spectrum of biological activities. Some of these compounds are on the list of clinical drugs, while others act as incredibly potent neurotoxins. Despite numerous attempts to prepare synthetic products, this review only introduces the natural diterpenoid alkaloids, describing ‘compounds’ structures and classifications and their toxicity and bioactivity. The purpose of the review is to highlight some existing relationships between the presence of substituents in the structure of such molecules and their recognised bioactivity.
Amin Thawabteh; Alà Thawabteh; Filomena Lelario; Sabino Bufo; Laura Scrano. Classification, Toxicity and Bioactivity of Natural Diterpenoid Alkaloids. Molecules 2021, 26, 4103 .
AMA StyleAmin Thawabteh, Alà Thawabteh, Filomena Lelario, Sabino Bufo, Laura Scrano. Classification, Toxicity and Bioactivity of Natural Diterpenoid Alkaloids. Molecules. 2021; 26 (13):4103.
Chicago/Turabian StyleAmin Thawabteh; Alà Thawabteh; Filomena Lelario; Sabino Bufo; Laura Scrano. 2021. "Classification, Toxicity and Bioactivity of Natural Diterpenoid Alkaloids." Molecules 26, no. 13: 4103.
The evolutionary arms race between plants and herbivores has led, over millions of years, to the production of many substances that prevent plants from being over-eaten by plant-feeding animals
Zbigniew Adamski; Sabino Bufo; Luigi Milella; Laura Scrano. Identification and Functional Characterization of Plant Toxins. Toxins 2021, 13, 228 .
AMA StyleZbigniew Adamski, Sabino Bufo, Luigi Milella, Laura Scrano. Identification and Functional Characterization of Plant Toxins. Toxins. 2021; 13 (3):228.
Chicago/Turabian StyleZbigniew Adamski; Sabino Bufo; Luigi Milella; Laura Scrano. 2021. "Identification and Functional Characterization of Plant Toxins." Toxins 13, no. 3: 228.
For thousands of years, Cannabis sativa has been utilized as a medicine and for recreational and spiritual purposes. Phytocannabinoids are a family of compounds that are found in the cannabis plant, which is known for its psychotogenic and euphoric effects; the main psychotropic constituent of cannabis is Δ9-tetrahydrocannabinol (Δ9-THC). The pharmacological effects of cannabinoids are a result of interactions between those compounds and cannabinoid receptors, CB1 and CB2, located in many parts of the human body. Cannabis is used as a therapeutic agent for treating pain and emesis. Some cannabinoids are clinically applied for treating chronic pain, particularly cancer and multiple sclerosis-associated pain, for appetite stimulation and anti-emesis in HIV/AIDS and cancer patients, and for spasticity treatment in multiple sclerosis and epilepsy patients. Medical cannabis varies from recreational cannabis in the chemical content of THC and cannabidiol (CBD), modes of administration, and safety. Despite the therapeutic effects of cannabis, exposure to high concentrations of THC, the main compound that is responsible for most of the intoxicating effects experienced by users, could lead to psychological events and adverse effects that affect almost all body systems, such as neurological (dizziness, drowsiness, seizures, coma, and others), ophthalmological (mydriasis and conjunctival hyperemia), cardiovascular (tachycardia and arterial hypertension), and gastrointestinal (nausea, vomiting, and thirst), mainly associated with recreational use. Cannabis toxicity in children is more concerning and can cause serious adverse effects such as acute neurological symptoms (stupor), lethargy, seizures, and even coma. More countries are legalizing the commercial production and sale of cannabis for medicinal use, and some for recreational use as well. Liberalization of cannabis laws has led to increased incidence of toxicity, hyperemesis syndrome, lung disease cardiovascular disease, reduced fertility, tolerance, and dependence with chronic prolonged use. This review focuses on the potential therapeutic effects of cannabis and cannabinoids, as well as the acute and chronic toxic effects of cannabis use on various body systems.
Zeinab Breijyeh; Buthaina Jubeh; Sabino Bufo; Rafik Karaman; Laura Scrano. Cannabis: A Toxin-Producing Plant with Potential Therapeutic Uses. Toxins 2021, 13, 117 .
AMA StyleZeinab Breijyeh, Buthaina Jubeh, Sabino Bufo, Rafik Karaman, Laura Scrano. Cannabis: A Toxin-Producing Plant with Potential Therapeutic Uses. Toxins. 2021; 13 (2):117.
Chicago/Turabian StyleZeinab Breijyeh; Buthaina Jubeh; Sabino Bufo; Rafik Karaman; Laura Scrano. 2021. "Cannabis: A Toxin-Producing Plant with Potential Therapeutic Uses." Toxins 13, no. 2: 117.
Organic waste is a rapidly increasing problem due to the growth of the agricultural production needed to meet global food demands. Development of sustainable waste management solutions is essential. Black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae) (BSF), larvae are voracious consumers of a wide range of organic materials ranging from fruits and vegetables to animal remains, and manure. Thanks to this ability and considering the larval high protein and lipid content, BSF larvae are a useful additive in animal feeds and biodiesel production. Unfortunately, the feasibility of using the black soldier fly as a tool for waste valorization and feed production has primarily been investigated at the benchtop scale. Thus, mobilization of current practices to an industrial scale is challenging because scaling up from small laboratory studies to large industrial studies is not necessarily linear. The goal of this study was to demonstrate the ability of the BSF to recycle organic waste at an industrial scale. To accomplish this goal, three organic waste streams were used (e.g., apples, bananas, and spent grain from a brewery) to test six diet treatments (1) apple, (2) banana, (3) spent grain, (4) apple and banana, (5) apple and spent grain, and (6) banana and spent grain. Working at scale of 10,000 BSF larvae life history traits, waste valorization, protein and lipid profiles were measured for each diet treatment. Differences were recorded across all variables, except substrate conversion, for larvae fed on fruit and spent grain (alone or with fruit). Growth rate significantly differed across treatments; larvae reared on spent grain grew twice as fast as those fed apples alone, but those reared on the apple and spent grain mixture produced twice as much insect biomass. However, it should be noted that larvae resulting from the apple diet contained 50% more fat than larvae fed the fruit and spent grain mixtures. Commonly-available organic wastes were successfully used at an industrial scale to produce BSF larvae that have the potential to substitute other sources of protein and lipids in different industrial applications. Industrialization efforts are encouraged to assess these impacts when integrating diverse ingredients into larval diets as a means to more precisely predict output, such as larval development time and final larval biomass.
Andrea Scala; Jonathan A. Cammack; Rosanna Salvia; Carmen Scieuzo; Antonio Franco; Sabino A. Bufo; Jeffery K. Tomberlin; Patrizia Falabella. Rearing substrate impacts growth and macronutrient composition of Hermetia illucens (L.) (Diptera: Stratiomyidae) larvae produced at an industrial scale. Scientific Reports 2020, 10, 1 -8.
AMA StyleAndrea Scala, Jonathan A. Cammack, Rosanna Salvia, Carmen Scieuzo, Antonio Franco, Sabino A. Bufo, Jeffery K. Tomberlin, Patrizia Falabella. Rearing substrate impacts growth and macronutrient composition of Hermetia illucens (L.) (Diptera: Stratiomyidae) larvae produced at an industrial scale. Scientific Reports. 2020; 10 (1):1-8.
Chicago/Turabian StyleAndrea Scala; Jonathan A. Cammack; Rosanna Salvia; Carmen Scieuzo; Antonio Franco; Sabino A. Bufo; Jeffery K. Tomberlin; Patrizia Falabella. 2020. "Rearing substrate impacts growth and macronutrient composition of Hermetia illucens (L.) (Diptera: Stratiomyidae) larvae produced at an industrial scale." Scientific Reports 10, no. 1: 1-8.
Synthetic insecticides are widely used for crop protection both in the fields and in the food stored facilities. Due to their toxicity, and assumptions of Integrated Pest Management, we conducted two independent experiments, where we studied the influence of Solanum nigrum unripe fruit extract on the toxicity of an organophosphorus insecticide fenitrothion. In the first variant of the experiment, Tenebrio molitor larvae were fed with blended fenitrothion (LC50) and the extract in four concentrations (0.01, 0.1, 1 and 10%) in ratio 1:1 for 3 days. In the second variant, a two-day application of fenitrothion (LC40) was preceded by a one-day extract treatment. The first variant did not show any increase in lethality compared to fenitrothion; however, ultrastructure observations exhibited swollen endoplasmic reticulum (ER) membranes in the midgut and nuclear and cellular membranes in the fat body, after application of blended fenitrothion and extract. An increased amount of heterochromatin in the fat body was observed, too. In the second variant, pre-treatment of the extract increased the lethality of larvae, decreased the level of glycogen and lipids in the fat body and disrupted integrity of midgut cellular membranes. S. nigrum extract, applied prior to fenitrothion treatment can be a factor increasing fenitrothion toxicity in T. molitor larvae. Thus, this strategy may lead to decreased emission of synthetic insecticides to the environment.
Marta Spochacz; Monika Szymczak; Szymon Chowański; Sabino Aurelio Bufo; Zbigniew Adamski. Solanum Nigrum Fruit Extract Increases Toxicity of Fenitrothion—A Synthetic Insecticide, in the Mealworm Beetle Tenebrio Molitor Larvae. Toxins 2020, 12, 612 .
AMA StyleMarta Spochacz, Monika Szymczak, Szymon Chowański, Sabino Aurelio Bufo, Zbigniew Adamski. Solanum Nigrum Fruit Extract Increases Toxicity of Fenitrothion—A Synthetic Insecticide, in the Mealworm Beetle Tenebrio Molitor Larvae. Toxins. 2020; 12 (10):612.
Chicago/Turabian StyleMarta Spochacz; Monika Szymczak; Szymon Chowański; Sabino Aurelio Bufo; Zbigniew Adamski. 2020. "Solanum Nigrum Fruit Extract Increases Toxicity of Fenitrothion—A Synthetic Insecticide, in the Mealworm Beetle Tenebrio Molitor Larvae." Toxins 12, no. 10: 612.
Nitrate and nitrite as sodium or potassium salts are usually added to meat products to develop the characteristic flavor, to inhibit the growth of microorganisms (particularly Clostridium botulinum), and effectively control rancidity by inhibiting lipid oxidation. However, both nitrate and nitrite ions need to be monitored for ensuring the quality and safety of cured meats. In this work, for the first time the content of nitrite and nitrate ions in homogenized meat samples of baby foods was determined by a validated method based on ion chromatography (IC) coupled with conductivity detection. Recoveries of nitrate and nitrite ions in meat samples were not lower than 84 ± 6%. The detection limits of nitrate and nitrite were 0.08 mg L−1 and 0.13 mg L−1, respectively. Five commercial samples of homogenized meat, namely lamb, rabbit, chicken, veal, and beef, for infant feeding were investigated; while nitrite content was below the detection limit, nitrate ranged from 10.7 to 21.0 mg kg−1. The results indicated that nitrate contents were below the European (EU) fixed value of 200 mg kg−1, and an acceptable daily intake of 3.7 mg kg−1 was estimated.
Donatella Coviello; Raffaella Pascale; Rosanna Ciriello; Anna Maria Salvi; Antonio Guerrieri; Michela Contursi; Laura Scrano; Sabino A. Bufo; Tommaso R.I. Cataldi; Giuliana Bianco. Validation of an Analytical Method for Nitrite and Nitrate Determination in Meat Foods for Infants by Ion Chromatography with Conductivity Detection. Foods 2020, 9, 1238 .
AMA StyleDonatella Coviello, Raffaella Pascale, Rosanna Ciriello, Anna Maria Salvi, Antonio Guerrieri, Michela Contursi, Laura Scrano, Sabino A. Bufo, Tommaso R.I. Cataldi, Giuliana Bianco. Validation of an Analytical Method for Nitrite and Nitrate Determination in Meat Foods for Infants by Ion Chromatography with Conductivity Detection. Foods. 2020; 9 (9):1238.
Chicago/Turabian StyleDonatella Coviello; Raffaella Pascale; Rosanna Ciriello; Anna Maria Salvi; Antonio Guerrieri; Michela Contursi; Laura Scrano; Sabino A. Bufo; Tommaso R.I. Cataldi; Giuliana Bianco. 2020. "Validation of an Analytical Method for Nitrite and Nitrate Determination in Meat Foods for Infants by Ion Chromatography with Conductivity Detection." Foods 9, no. 9: 1238.
Commercial non-steroidal anti-inflammatory drugs (NSAIDs) are considered as toxic to the environment since they induce side effects when consumed by humans or aquatic life. Ibuprofen is a member of the NSAID family and is widely used as an anti-inflammatory and painkiller agent. Photolysis is a potentially important method of degradation for several emerging contaminants, and individual compounds can undergo photolysis to various degrees, depending on their chemical structure. The efficiency oftitanium dioxide (TiO2) and photocatalysis was investigated for the removal of ibuprofen from the aquatic environment, and the performance of these different processes was evaluated. In heterogeneous photocatalysis, two experiments were carried out using TiO2 as (i) dispersed powder, and (ii) TiO2 immobilized on the active surface of commercial coated glass. The kinetics of each photoreaction was determined, and the identification of the photoproducts was carried out by liquid chromatography coupled with Fourier-transform ion cyclotron resonance mass spectrometry (LC-FTICR MS). The overall results suggest that the TiO2 active thin layer immobilized on the glass substrate can avoid recovery problems related to the use of TiO2 powder in heterogeneous photocatalysis and may be a promising tool toward protecting the environment from emerging contaminants such as ibuprofen and its derivatives.
Samer Khalaf; Jawad H. Shoqeir; Filomena Lelario; Sabino A. Bufo; Rafik Karaman; Laura Scrano. TiO2 and Active Coated Glass Photodegradation of Ibuprofen. Catalysts 2020, 10, 560 .
AMA StyleSamer Khalaf, Jawad H. Shoqeir, Filomena Lelario, Sabino A. Bufo, Rafik Karaman, Laura Scrano. TiO2 and Active Coated Glass Photodegradation of Ibuprofen. Catalysts. 2020; 10 (5):560.
Chicago/Turabian StyleSamer Khalaf; Jawad H. Shoqeir; Filomena Lelario; Sabino A. Bufo; Rafik Karaman; Laura Scrano. 2020. "TiO2 and Active Coated Glass Photodegradation of Ibuprofen." Catalysts 10, no. 5: 560.
The power of Mass spectrometry (MS) coupled to liquid chromatography (LC–MS) with regard to the characterization of food matrices is presented. LC-MS is a very powerful analytical tool since it can provide direct qualitative and quantitative information about target molecules of interest. Quality of this information is influenced by the sample preparation procedure, the type of LC-MS used and the availability of dedicated database. This article highlights an overview of the applicability using LC-MS in various foods covering target and nontarget approaches. Furthermore, a brief description of the main food database, available as web-based resources, is presented.
Raffaella Pascale; Alberto Onzo; Rosanna Ciriello; Laura Scrano; Sabino A. Bufo; Giuliana Bianco. LC/MS Based Food Metabolomics. Comprehensive Foodomics 2020, 39 -53.
AMA StyleRaffaella Pascale, Alberto Onzo, Rosanna Ciriello, Laura Scrano, Sabino A. Bufo, Giuliana Bianco. LC/MS Based Food Metabolomics. Comprehensive Foodomics. 2020; ():39-53.
Chicago/Turabian StyleRaffaella Pascale; Alberto Onzo; Rosanna Ciriello; Laura Scrano; Sabino A. Bufo; Giuliana Bianco. 2020. "LC/MS Based Food Metabolomics." Comprehensive Foodomics , no. : 39-53.
Plants produce many secondary metabolites, which reveal biological activity
Zbigniew Adamski; Linda L. Blythe; Luigi Milella; Sabino A. Bufo. Biological Activities of Alkaloids: From Toxicology to Pharmacology. Toxins 2020, 12, 210 .
AMA StyleZbigniew Adamski, Linda L. Blythe, Luigi Milella, Sabino A. Bufo. Biological Activities of Alkaloids: From Toxicology to Pharmacology. Toxins. 2020; 12 (4):210.
Chicago/Turabian StyleZbigniew Adamski; Linda L. Blythe; Luigi Milella; Sabino A. Bufo. 2020. "Biological Activities of Alkaloids: From Toxicology to Pharmacology." Toxins 12, no. 4: 210.
Interest in targeted profiling of quercetin glycoconjugates occurring in edible foodstuffs continues to expand because of their recognized beneficial health effects. Quercetin derivatives encompass several thousands of chemically distinguishable compounds, among which there are several compounds with different glycosylations and acylations. Since reference standards and dedicated databases are not available, the mass spectrometric identification of quercetin glycoconjugates is challenging. A targeted liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) was applied for screening quercetin glycoconjugates in edible peperoni di Senise peppers (Capsicum annuum L.), protected by the European Union with the mark PGI (i.e., Protected Geographical Indication), and cultivated in Basilicata (Southern Italy). Chromatographic separation was accomplished by reversed-phase liquid chromatography (RPLC) using water/acetonitrile as the mobile phase and detection was performed on a linear ion trap mass spectrometer fitted with an electrospray ionization (ESI) source operating in negative ion mode. A correlation between experimental RP chromatographic retention time and those predicted by partition coefficients (log P) along with MS/MS data and an in-house developed database (named QUEdb) provided deep coverage for sixteen quercetin glycoconjugates. Among them, eleven quercetin glycoconjugates were already described in the literature and five were reported for the first time. These last acyl glycosidic quercetin derivatives were tentatively identified as quercetin-(galloyl-rhamnoside)-hexoside, [C34H33O20]− at m/z 761.1; quercetin-(sinapoyl-hexoside)-rhamnoside, [C38H39O20]− at m/z 815.4; quercetin-(galloyl-caffeoyl-hexoside)-rhamnoside, [C43H39O23]− at m/z 923.0; quercetin-(feruloyl-hexoside)-rhamnoside, [C37H37O19]− at m/z 785.1; and quercetin-(succinyl-rhamnoside)-rhamnoside, [C31H33O18]− at m/z 693.1. Graphical abstract
Raffaella Pascale; Maria A. Acquavia; Tommaso R. I. Cataldi; Alberto Onzo; Donatella Coviello; Sabino A. Bufo; Laura Scrano; Rosanna Ciriello; Antonio Guerrieri; Giuliana Bianco. Profiling of quercetin glycosides and acyl glycosides in sun-dried peperoni di Senise peppers (Capsicum annuum L.) by a combination of LC-ESI(-)-MS/MS and polarity prediction in reversed-phase separations. Analytical and Bioanalytical Chemistry 2020, 412, 3005 -3015.
AMA StyleRaffaella Pascale, Maria A. Acquavia, Tommaso R. I. Cataldi, Alberto Onzo, Donatella Coviello, Sabino A. Bufo, Laura Scrano, Rosanna Ciriello, Antonio Guerrieri, Giuliana Bianco. Profiling of quercetin glycosides and acyl glycosides in sun-dried peperoni di Senise peppers (Capsicum annuum L.) by a combination of LC-ESI(-)-MS/MS and polarity prediction in reversed-phase separations. Analytical and Bioanalytical Chemistry. 2020; 412 (12):3005-3015.
Chicago/Turabian StyleRaffaella Pascale; Maria A. Acquavia; Tommaso R. I. Cataldi; Alberto Onzo; Donatella Coviello; Sabino A. Bufo; Laura Scrano; Rosanna Ciriello; Antonio Guerrieri; Giuliana Bianco. 2020. "Profiling of quercetin glycosides and acyl glycosides in sun-dried peperoni di Senise peppers (Capsicum annuum L.) by a combination of LC-ESI(-)-MS/MS and polarity prediction in reversed-phase separations." Analytical and Bioanalytical Chemistry 412, no. 12: 3005-3015.
Pharmaceuticals constitute one of the most important emerging classes of environmental pollutants. A three‐phase solvent system of water, water containing 0.1% of formic acid and acetonitrile was successfully used to separate, by LC‐MS, polarity‐matched pharmaceuticals, i.e., carbamazepine, clarithromycin and erythromycin, as well as amoxicillin and metformin. Despite of polarity similarities, these pharmaceuticals were completely resolved in the analytical run time of 15 minutes. The optimized three‐phase solvent system based‐method was validated for the simultaneous analysis of 6 matched‐polarity pharmaceuticals in wastewater samples. Good linearity (coefficient of determination more than 0.993) and precision (relative standard deviation less than 15.66%) were achieved. Recovery of analytes from the wastewater was between 0.70 and 1.18. Limits of detections ranged from 0.0001 μg/l to 0.5114 μg/l. No significant matrix effect, evaluated by post extraction addition, was observed in the ESI source. Then, this methodology has been successfully applied to environmental study of pharmaceutical residues occurring in influent and effluent wastewater samples, from the main wastewater treatment plant in Potenza (Basilicata, Southern Italy). This article is protected by copyright. All rights reserved
Raffaella Pascale; Giuliana Bianco; Donatella Coviello; Maria Cristina Lafiosca; Salvatore Masi; Ignazio M. Mancini; Sabino A. Bufo; Laura Scrano; Donatella Caniani. Validation of a liquid chromatography coupled with tandem mass spectrometry method for the determination of drugs in wastewater using a three‐phase solvent system. Journal of Separation Science 2019, 43, 886 -895.
AMA StyleRaffaella Pascale, Giuliana Bianco, Donatella Coviello, Maria Cristina Lafiosca, Salvatore Masi, Ignazio M. Mancini, Sabino A. Bufo, Laura Scrano, Donatella Caniani. Validation of a liquid chromatography coupled with tandem mass spectrometry method for the determination of drugs in wastewater using a three‐phase solvent system. Journal of Separation Science. 2019; 43 (5):886-895.
Chicago/Turabian StyleRaffaella Pascale; Giuliana Bianco; Donatella Coviello; Maria Cristina Lafiosca; Salvatore Masi; Ignazio M. Mancini; Sabino A. Bufo; Laura Scrano; Donatella Caniani. 2019. "Validation of a liquid chromatography coupled with tandem mass spectrometry method for the determination of drugs in wastewater using a three‐phase solvent system." Journal of Separation Science 43, no. 5: 886-895.
Many Burkholderia spp. produce in vitro secondary metabolites with relevant biological activities and potential practical applications. Burkholderia gladioli pv. agaricicola (Bga) possess promising biological activities regulated by N-Acyl homoserine lactones (N.AHLs) based quorum sensing (QS) mechanism. In the current study, N.AHLs-deficient (ICMP11096glad-I) and N.AHLs-complemented (ICMP11096glad-IR) mutants were constructed in which the gene coding for AHL synthase was inactivated by allelic exchange in glad I mutant strain. The aims of this research were to (i) assess the antagonistic activity of the wild type (WT) and the glad-I mutant of Bga against Bacillus megaterium (G+ve) and Escherichia coli (G−ve), (ii) screen their hydrolytic enzymes and hemolytic substances, (iii) monitor the pathogenic effect against Agaricus bisporus, and finally (iv) analyze the bioactive secondary metabolites produced by WT and mutant strain using high performance liquid chromatography (HPLC). Results showed that N.AHLs-deficient mutant exhibited high reduction of antagonistic activity against the tested microorganisms and notable reduction of chitinolytic, proteolytic and glucanolytic activities and complete absence of hemolytic activity, and the glad-IR complemented mutant was able to regain the major part of these activities. Furthermore, N.AHLs-deficient mutant strain was unable to degrade flesh cubes pseudo-tissues of A. bisporus. On the other hand, the virulence effect of complemented mutant was like to the parental WT strain. HPLC analysis revealed that some of the single components produced by WT strain were absent in N.AHLs-deficient mutant and others were highly reduced. The out-findings of the current research gave a spot into the regulatory role of N.AHLs and QS phenomenon in the biological activity of Bga bacterium.
Hazem S. Elshafie; Giulia Devescovi; Vittorio Venturi; Ippolito Camele; Sabino A. Bufo. Study of the Regulatory Role of N-Acyl Homoserine Lactones Mediated Quorum Sensing in the Biological Activity of Burkholderia gladioli pv. agaricicola Causing Soft Rot of Agaricus spp. Frontiers in Microbiology 2019, 10, 2695 .
AMA StyleHazem S. Elshafie, Giulia Devescovi, Vittorio Venturi, Ippolito Camele, Sabino A. Bufo. Study of the Regulatory Role of N-Acyl Homoserine Lactones Mediated Quorum Sensing in the Biological Activity of Burkholderia gladioli pv. agaricicola Causing Soft Rot of Agaricus spp. Frontiers in Microbiology. 2019; 10 ():2695.
Chicago/Turabian StyleHazem S. Elshafie; Giulia Devescovi; Vittorio Venturi; Ippolito Camele; Sabino A. Bufo. 2019. "Study of the Regulatory Role of N-Acyl Homoserine Lactones Mediated Quorum Sensing in the Biological Activity of Burkholderia gladioli pv. agaricicola Causing Soft Rot of Agaricus spp." Frontiers in Microbiology 10, no. : 2695.
The growing incidence of microorganisms that resist antimicrobials is a constant concern for the scientific community, while the development of new antimicrobials from new chemical entities has become more and more expensive, time-consuming, and exacerbated by emerging drug-resistant strains. In this regard, many scientists are conducting research on plants aiming to discover possible antimicrobial compounds. The secondary metabolites contained in plants are a source of chemical entities having pharmacological activities and intended to be used for the treatment of different diseases. These chemical entities have the potential to be used as an effective antioxidant, antimutagenic, anticarcinogenic and antimicrobial agents. Among these pharmacologically active entities are the alkaloids which are classified into a number of classes, including pyrrolizidines, pyrrolidines, quinolizidines, indoles, tropanes, piperidines, purines, imidazoles, and isoquinolines. Alkaloids that have antioxidant properties are capable of preventing a variety of degenerative diseases through capturing free radicals, or through binding to catalysts involved indifferent oxidation processes occurring within the human body. Furthermore, these entities are capable of inhibiting the activity of bacteria, fungi, protozoan and etc. The unique properties of these secondary metabolites are the main reason for their utilization by the pharmaceutical companies for the treatment of different diseases. Generally, these alkaloids are extracted from plants, animals and fungi. Penicillin is the most famous natural drug discovery deriving from fungus. Similarly, marines have been used as a source for thousands of bioactive marine natural products. In this review, we cover the medical use of natural alkaloids isolated from a variety of plants and utilized by humans as antibacterial, antiviral, antifungal and anticancer agents. An example for such alkaloids is berberine, an isoquinoline alkaloid, found in roots and stem-bark of Berberis asculin P. Renault plant and used to kill a variety of microorganisms.
Amin Thawabtah; Salma Juma; Mariam Bader; Donia Karaman; Laura Scrano; Sabino A. Bufo; Rafik Karaman. The Biological Activity of Natural Alkaloids against Herbivores, Cancerous Cells and Pathogens. Toxins 2019, 11, 656 .
AMA StyleAmin Thawabtah, Salma Juma, Mariam Bader, Donia Karaman, Laura Scrano, Sabino A. Bufo, Rafik Karaman. The Biological Activity of Natural Alkaloids against Herbivores, Cancerous Cells and Pathogens. Toxins. 2019; 11 (11):656.
Chicago/Turabian StyleAmin Thawabtah; Salma Juma; Mariam Bader; Donia Karaman; Laura Scrano; Sabino A. Bufo; Rafik Karaman. 2019. "The Biological Activity of Natural Alkaloids against Herbivores, Cancerous Cells and Pathogens." Toxins 11, no. 11: 656.
The molecular bases of the host-parasitoid interactions in the biological system Acyrthosiphon pisum (Harris) (Homoptera, Aphididae) and Aphidius ervi (Haliday) (Hymenoptera, Braconidae) have been elucidated allowing the identification of a gamma-glutamyl transpeptidase, the active component of maternal venom secretion, and teratocytes, the embryonic parasitic factors responsible for host physiology regulation after parasitization. Teratocytes, cells deriving from the dissociation of the serosa, the parasitoid embryonic membrane, are responsible for extra-oral digestion of host tissues in order to provide a suitable nutritional environment for the development of parasitoid larvae. Teratocytes rapidly grow in size without undergoing any cell division, synthesize, and release in the host hemolymph two proteins: a fatty acid binding protein (Ae-FABP) and an enolase (Ae-ENO). Ae-FABP is involved in transport of fatty acids deriving from host tissues to the parasitoid larva. Ae-ENO is an extracellular glycolytic enzyme that functions as a plasminogen like receptor inducing its activation to plasmin. Both Ae-FABP and Ae-ENO lack their signal peptides, and they are released in the extracellular environment through an unknown secretion pathway. Here, we investigated the unconventional mechanism by which teratocytes release Ae-FABP and Ae-ENO in the extracellular space. Our results, obtained using immunogold staining coupled with TEM and western blot analyses, show that these two proteins are localized in vesicles released by teratocytes. The specific dimension of these vesicles and the immunodetection of ALIX and HSP70, two exosome markers, strongly support the hypothesis that these vesicles are exosomes.
Rosanna Salvia; Annalisa Grimaldi; Rossana Girardello; Carmen Scieuzo; Andrea Scala; Sabino A. Bufo; Heiko Vogel; Patrizia Falabella. Aphidius ervi Teratocytes Release Enolase and Fatty Acid Binding Protein Through Exosomal Vesicles. Frontiers in Physiology 2019, 10, 1 .
AMA StyleRosanna Salvia, Annalisa Grimaldi, Rossana Girardello, Carmen Scieuzo, Andrea Scala, Sabino A. Bufo, Heiko Vogel, Patrizia Falabella. Aphidius ervi Teratocytes Release Enolase and Fatty Acid Binding Protein Through Exosomal Vesicles. Frontiers in Physiology. 2019; 10 ():1.
Chicago/Turabian StyleRosanna Salvia; Annalisa Grimaldi; Rossana Girardello; Carmen Scieuzo; Andrea Scala; Sabino A. Bufo; Heiko Vogel; Patrizia Falabella. 2019. "Aphidius ervi Teratocytes Release Enolase and Fatty Acid Binding Protein Through Exosomal Vesicles." Frontiers in Physiology 10, no. : 1.
This study focuses on several aspects of communication strategies adopted by adults of the Mediterranean flat-headed root-borer Capnodis tenebrionis (Coleoptera: Buprestidae). Morphological studies on the structures involved in mate recognition and acceptance revealed the presence of porous areas in the pronota in both sexes. These areas were variable in shape and size, but proportionally larger in males. The presence of chaetic, basiconic, and coeloconic sensilla in the antennae of both males and females was verified. Bioassays revealed stereotyped rituals in males and the involvement of female pronotal secretions in mate recognition and acceptance. During the mating assays, the female’s pronotum was covered by a biologically inert polymeric resin (DenFilTM), which prevented males from detecting the secretions and from completing the copulation ritual. The use of the resin allowed for the collection of chemical compounds. GC-MS analysis of the resin suggested it may be used to retain compounds from insect body surfaces and revealed sex-specific chemical profiles in the cuticles. Since adult C. tenebrionis may use volatile organic compounds (VOCs) emitted from leaves or shoots, the VOC emission profiles of apricot trees were characterized. Several volatiles related to plant-insect interactions involving fruit tree species of the Rosaceae family and buprestid beetles were identified. To improve understanding of how VOCs are perceived, candidate soluble olfactory proteins involved in chemoreception (odorant-binding proteins and chemosensory proteins) were identified using tissue and sex-specific RNA-seq data. The implications for chemical identification, physiological and ecological functions in intraspecific communication and insect–host interactions are discussed and potential applications for monitoring presented.
Giuseppe Bari; Andrea Scala; Vita Garzone; Rosanna Salvia; Cem Yalcin; Pasqua Vernile; Antonella Maria Aresta; Osvaldo Facini; Rita Baraldi; Sabino A. Bufo; Heiko Vogel; Enrico De Lillo; Francesca Rapparini; Patrizia Falabella. Chemical Ecology of Capnodis tenebrionis (L.) (Coleoptera: Buprestidae): Behavioral and Biochemical Strategies for Intraspecific and Host Interactions. Frontiers in Physiology 2019, 10, 1 .
AMA StyleGiuseppe Bari, Andrea Scala, Vita Garzone, Rosanna Salvia, Cem Yalcin, Pasqua Vernile, Antonella Maria Aresta, Osvaldo Facini, Rita Baraldi, Sabino A. Bufo, Heiko Vogel, Enrico De Lillo, Francesca Rapparini, Patrizia Falabella. Chemical Ecology of Capnodis tenebrionis (L.) (Coleoptera: Buprestidae): Behavioral and Biochemical Strategies for Intraspecific and Host Interactions. Frontiers in Physiology. 2019; 10 ():1.
Chicago/Turabian StyleGiuseppe Bari; Andrea Scala; Vita Garzone; Rosanna Salvia; Cem Yalcin; Pasqua Vernile; Antonella Maria Aresta; Osvaldo Facini; Rita Baraldi; Sabino A. Bufo; Heiko Vogel; Enrico De Lillo; Francesca Rapparini; Patrizia Falabella. 2019. "Chemical Ecology of Capnodis tenebrionis (L.) (Coleoptera: Buprestidae): Behavioral and Biochemical Strategies for Intraspecific and Host Interactions." Frontiers in Physiology 10, no. : 1.
The usage of insects as model organisms is becoming more and more common in toxicological, pharmacological, genetic and biomedical research. Insects, such as fruit flies (Drosophila melanogaster), locusts (Locusta migratoria), stick insects (Baculum extradentatum) or beetles (Tenebrio molitor) are used to assess the effect of different active compounds, as well as to analyse the background and course of certain diseases, including heart disorders. The goal of this study was to assess the influence of secondary metabolites extracted from Solanaceae and Brassicaceae plants: Potato (Solanum tuberosum), tomato (Solanum lycopersicum), black nightshade (Solanum nigrum) and horseradish (Armoracia rusticana), on T. molitor beetle heart contractility in comparison with pure alkaloids. During the in vivo bioassays, the plants glycoalkaloid extracts and pure substances were injected at the concentration 10-5 M into T. molitor pupa and evoked changes in heart activity. Pure glycoalkaloids caused mainly positive chronotropic effects, dependant on heart activity phase during a 24-h period of recording. Moreover, the substances affected the duration of the heart activity phases. Similarly, to the pure glycoalkaloids, the tested extracts also mainly accelerated the heart rhythm, however S. tuberosum and S. lycopersicum extracts slightly decreased the heart contractions frequency in the last 6 h of the recording. Cardioacceleratory activity of only S. lycopersicum extract was higher than single alkaloids whereas S. tubersoum and S. nigrum extracts were less active when compared to pure alkaloids. The most cardioactive substance was chaconine which strongly stimulated heart action during the whole recording after injection. A. rusticana extract which is composed mainly of glucosinolates did not significantly affect the heart contractions. Obtained results showed that glycoalkaloids were much more active than glucosinolates. However, the extracts depending on the plant species might be more or less active than pure substances.
Paweł Marciniak; Angelika Kolińska; Marta Spochacz; Szymon Chowański; Zbigniew Adamski; Laura Scrano; Patrizia Falabella; Sabino A. Bufo; Grzegorz Rosiński. Differentiated Effects of Secondary Metabolites from Solanaceae and Brassicaceae Plant Families on the Heartbeat of Tenebrio molitor Pupae. Toxins 2019, 11, 287 .
AMA StylePaweł Marciniak, Angelika Kolińska, Marta Spochacz, Szymon Chowański, Zbigniew Adamski, Laura Scrano, Patrizia Falabella, Sabino A. Bufo, Grzegorz Rosiński. Differentiated Effects of Secondary Metabolites from Solanaceae and Brassicaceae Plant Families on the Heartbeat of Tenebrio molitor Pupae. Toxins. 2019; 11 (5):287.
Chicago/Turabian StylePaweł Marciniak; Angelika Kolińska; Marta Spochacz; Szymon Chowański; Zbigniew Adamski; Laura Scrano; Patrizia Falabella; Sabino A. Bufo; Grzegorz Rosiński. 2019. "Differentiated Effects of Secondary Metabolites from Solanaceae and Brassicaceae Plant Families on the Heartbeat of Tenebrio molitor Pupae." Toxins 11, no. 5: 287.
Prothoracicotropic hormone (PTTH) is a neuropeptide that triggers a cascade of events within the prothoracic gland (PG) cells, leading to the activation of all the crucial enzymes involved in ecdysone biosynthesis, the main insect steroid hormone. Studies concerning ecdysteroidogenesis predicted PTTH action using brain extract (BE), consisting in a complex mixture in which some components positively or negatively interfere with PTTH-stimulated ecdysteroidogenesis. Consequently, the integration of these opposing factors in steroidogenic tissues leads to a complex secretory pattern. A recombinant form of prothoracicotropic hormone (rPTTH) from the tobacco budworm Heliothis virescens (F.) (Lepidoptera: Noctuidae) was expressed and purified to perform in vitro tests in a standard and repeatable manner. A characterization of rPTTH primary and secondary structures was performed. The ability of rPTTH and H. virescens BE to stimulate ecdysteroidogenesis was investigated on the third day of fifth larval stage. rPTTH activity was compared with the BE mixture by enzyme immunoassay and western blot, revealing that they equally stimulate the production of significant amount of ecdysone, through a transduction cascade that includes the TOR pathway, by the phosphorylation of 4E binding protein (4E-BP) and S6 kinase (S6K), the main targets of TOR protein. The results of these experiments suggest the importance of obtaining a functional pure hormone to perform further studies, not depending on the crude brain extract, composed by different elements and susceptible to different uncontrollable variables.
Marisa Nardiello; Rosanna Salvia; Andrea Scala; Carmen Scieuzo; Sabino Aurelio Bufo; Antonio Franco; Heiko Vogel; Angela di Somma; Angela Duilio; Patrizia Falabella. Ecdysteroidogenesis in Heliothis virescens (Lepidoptera: Noctuidae): Recombinant Prothoracicotropic Hormone and Brain Extract Show Comparable Effects. Journal of Insect Science 2019, 19, 1 .
AMA StyleMarisa Nardiello, Rosanna Salvia, Andrea Scala, Carmen Scieuzo, Sabino Aurelio Bufo, Antonio Franco, Heiko Vogel, Angela di Somma, Angela Duilio, Patrizia Falabella. Ecdysteroidogenesis in Heliothis virescens (Lepidoptera: Noctuidae): Recombinant Prothoracicotropic Hormone and Brain Extract Show Comparable Effects. Journal of Insect Science. 2019; 19 (3):1.
Chicago/Turabian StyleMarisa Nardiello; Rosanna Salvia; Andrea Scala; Carmen Scieuzo; Sabino Aurelio Bufo; Antonio Franco; Heiko Vogel; Angela di Somma; Angela Duilio; Patrizia Falabella. 2019. "Ecdysteroidogenesis in Heliothis virescens (Lepidoptera: Noctuidae): Recombinant Prothoracicotropic Hormone and Brain Extract Show Comparable Effects." Journal of Insect Science 19, no. 3: 1.
Eggplant contains glycoalkaloids (GAs), a class of nitrogen-containing secondary metabolites of great structural variety that may have both adverse and beneficial biological effects. In this study, we performed a complete survey of GAs and their malonylated form, in two genotypes of eggplants: A commercial cultivated type, Mirabella (Mir), with purple peel and bitter taste and a local landrace, named Melanzana Bianca di Senise (Sen), characterized by white peel with purple strip and a typical sweet aroma. Besides the analysis of their morphological traits, nineteen glycoalkaloids were tentatively identified in eggplant berry extracts based upon LC-ESI-FTICR-MS analysis using retention times, elution orders, high-resolution mass spectra, as well as high-resolution fragmentation by IRMPD. The relative signal intensities (i.e., ion counts) of the GAs identified in Mir and Sen pulp extracts showed as solamargine, and its isomers are the most abundant. In addition, anticholinesterase and antioxidant activities of the extracts were evaluated. Pulp tissue was found to be more active in inhibiting acetylcholinesterase enzyme than peel showing an inhibitory effect higher than 20% for Mir pulp. The identification of new malonylated GAs in eggplant is proposed.
Filomena Lelario; Susanna De Maria; Anna Rita Rivelli; Daniela Russo; Luigi Milella; Sabino Aurelio Bufo; Laura Scrano. A Complete Survey of Glycoalkaloids Using LC-FTICR-MS and IRMPD in a Commercial Variety and a Local Landrace of Eggplant (Solanum melongena L.) and their Anticholinesterase and Antioxidant Activities. Toxins 2019, 11, 230 .
AMA StyleFilomena Lelario, Susanna De Maria, Anna Rita Rivelli, Daniela Russo, Luigi Milella, Sabino Aurelio Bufo, Laura Scrano. A Complete Survey of Glycoalkaloids Using LC-FTICR-MS and IRMPD in a Commercial Variety and a Local Landrace of Eggplant (Solanum melongena L.) and their Anticholinesterase and Antioxidant Activities. Toxins. 2019; 11 (4):230.
Chicago/Turabian StyleFilomena Lelario; Susanna De Maria; Anna Rita Rivelli; Daniela Russo; Luigi Milella; Sabino Aurelio Bufo; Laura Scrano. 2019. "A Complete Survey of Glycoalkaloids Using LC-FTICR-MS and IRMPD in a Commercial Variety and a Local Landrace of Eggplant (Solanum melongena L.) and their Anticholinesterase and Antioxidant Activities." Toxins 11, no. 4: 230.
Model organisms are often used in biological, medical and environmental research. Among insects, Drosophila melanogaster, Galleria mellonella, Apis mellifera, Bombyx mori, Periplaneta americana, and Locusta migratoria are often used. However, new model organisms still appear. In recent years, an increasing number of insect species has been suggested as model organisms in life sciences research due to their worldwide distribution and environmental significance, the possibility of extrapolating research studies to vertebrates and the relatively low cost of rearing. Beetles are the largest insect order, with their representative – Tribolium castaneum – being the first species with a completely sequenced genome, and seem to be emerging as new potential candidates for model organisms in various studies. Apart from T. castaneum, additional species representing various Coleoptera families, such as Nicrophorus vespilloides, Leptinotarsa decemlineata, Coccinella septempunctata, Poecilus cupreus, Tenebrio molitor and many others, have been used. They are increasingly often included in two major research aspects: biomedical and environmental studies. Biomedical studies focus mainly on unraveling mechanisms of basic life processes, such as feeding, neurotransmission or activity of the immune system, as well as on elucidating the mechanism of different diseases (neurodegenerative, cardiovascular, metabolic, or immunological) using beetles as models. Furthermore, pharmacological bioassays for testing novel biologically active substances in beetles have also been developed. It should be emphasized that beetles are a source of compounds with potential antimicrobial and anticancer activity. Environmental-based studies focus mainly on the development and testing of new potential pesticides of both chemical and natural origin. Additionally, beetles are used as food or for their valuable supplements. Different beetle families are also used as bioindicators. Another important research area using beetles as models is behavioral ecology studies, for instance, parental care. In this paper, we review the current knowledge regarding beetles as model organisms and their practical application in various fields of life science.
Zbigniew Adamski; Sabino A. Bufo; Szymon Chowański; Patrizia Falabella; Jan Lubawy; Pawel Marciniak; Joanna Pacholska-Bogalska; Rosanna Salvia; Laura Scrano; Małgorzata Słocińska; Marta Spochacz; Monika Szymczak; Arkadiusz Urbański; Karolina Walkowiak-Nowicka; Grzegorz Rosiński. Beetles as Model Organisms in Physiological, Biomedical and Environmental Studies – A Review. Frontiers in Physiology 2019, 10, 319 .
AMA StyleZbigniew Adamski, Sabino A. Bufo, Szymon Chowański, Patrizia Falabella, Jan Lubawy, Pawel Marciniak, Joanna Pacholska-Bogalska, Rosanna Salvia, Laura Scrano, Małgorzata Słocińska, Marta Spochacz, Monika Szymczak, Arkadiusz Urbański, Karolina Walkowiak-Nowicka, Grzegorz Rosiński. Beetles as Model Organisms in Physiological, Biomedical and Environmental Studies – A Review. Frontiers in Physiology. 2019; 10 ():319.
Chicago/Turabian StyleZbigniew Adamski; Sabino A. Bufo; Szymon Chowański; Patrizia Falabella; Jan Lubawy; Pawel Marciniak; Joanna Pacholska-Bogalska; Rosanna Salvia; Laura Scrano; Małgorzata Słocińska; Marta Spochacz; Monika Szymczak; Arkadiusz Urbański; Karolina Walkowiak-Nowicka; Grzegorz Rosiński. 2019. "Beetles as Model Organisms in Physiological, Biomedical and Environmental Studies – A Review." Frontiers in Physiology 10, no. : 319.