Inspired by natural porous architectures, numerous attempts have been made to generate porous structures. Owing to the smooth surfaces, highly interconnected porous architectures, and mathematical controllable geometry features, triply periodic minimal surface (TPMS) is emerging as an outstanding solution to constructing porous structures in recent years. However, many advantages of TPMS are not fully utilized in current research. Critical problems of the process from design, manufacturing to applications need further systematic and integrated discussions. In this work, a comprehensive overview of TPMS porous structures is provided. In order to generate the digital models of TPMS, the geometry design algorithms and performance control strategies are introduced according to diverse requirements. Based on that, precise additive manufacturing methods are summarized for fabricating physical TPMS products. Furthermore, actual multidisciplinary applications are presented to clarify the advantages and further potential of TPMS porous structures. Eventually, the existing problems and further research outlooks are discussed.

As the biocompatible materials, hydrogels have been widely used in three- dimensional (3D) bioprinting/organ printing to load cell for tissue engineering. It is important to precisely control hydrogels deposition during printing the mimic organ structures. However, the printability of hydrogels about printing parameters is seldom addressed. In this paper, we systemically investigated the printability of hydrogels from printing lines (one dimensional, 1D structures) to printing lattices/films (two dimensional, 2D structures) and printing 3D structures with a special attention to the accurate printing. After a series of experiments, we discovered the relationships between the important factors such as air pressure, feedrate, or even printing distance and the printing quality of the expected structures. Dumbbell shape was observed in the lattice structures printing due to the hydrogel diffuses at the intersection. Collapses and fusion of adjacent layer would result in the error accumulation at Z direction which was an important fact that could cause printing failure. Finally, we successfully demonstrated a 3D printing hydrogel scaffold through harmonize with all the parameters. The cell viability after printing was compared with the casting and the results showed that our bioprinting method almost had no extra damage to the cells.

Biomanufacturing of tissues/organs in vitro is our big dream, driven by two needs: organ transplantation and accurate tissue models. Over the last decades, 3D bioprinting has been widely applied in the construction of many tissues/organs such as skins, vessels, hearts, etc., which can not only lay a foundation for the grand goal of organ replacement, but also be served as in vitro models committed to pharmacokinetics, drug screening and so on. As organs are so complicated, many bioprinting methods are exploited to figure out the challenges of different applications. So the question is how to choose the suitable bioprinting method? Herein, we systematically review the evolution, process and classification of 3D bioprinting with an emphasis on the fundamental printing principles and commercialized bioprinters. We summarize and classify extrusion-based, droplet-based, and photocuring-based bioprinting methods and give some advices for applications. Among them, coaxial and multi-material bioprinting are highlighted and basic principles of designing bioinks are also discussed.