Toxin production during harmful algal blooms (HABs) depends on N availability. However, the role of N₂ fixation as a mechanism to fuel ‘new’ N into HABs and increase their toxicity has not been well studied. We quantified the effects of N∶P supply ratios on N₂-fixation efficiency in HABs from 3 warm-temperate man-made reservoirs. We enriched mesocosms with the same concentration of P (112 µg P/L) but differing amounts of N (50‒2500 µg N/L) labeled with a ¹⁵N tracer to simulate HABs growing along a large molar N∶P gradient (1‒50). N₂ fixation increased significantly at low N∶P but generally did not alleviate N limitation and lead to accumulating N and phytoplankton biomass efficiently unless the magnitude of stoichiometric imbalance was low. Furthermore, microcystin concentrations >1.0 µg/L occurred only in mesocosms receiving N∶P = 50 supply and only in the reservoir with detectable concentrations of microcystin at the beginning of the experiment. These results suggest that HABs in P-rich reservoirs may yield significantly more biomass and potentially become more toxic when reactive N is plentiful in the water column relative to P. Thus, reducing N concentrations can be useful as a supplement to the primary P reduction strategies used to minimize the harmful effects of algal blooms.

Debate remains about the effectiveness of commercial thinning as a wildfire management strategy, with some studies reporting somewhat lower severity in thinned forests, and some reporting higher severity, during wildfires. However, while vegetation severity is a measure of basal area tree mortality, research on this question generally omits tree mortality from thinning itself. We investigated whether cumulative tree mortality, or cumulative severity, from commercial thinning and wildfire was different between thinned and unthinned forests in the Caldor Fire of 2021 in the northern Sierra Nevada mountains of California, USA. We found significantly higher cumulative severity in commercial thinning areas compared to unthinned forests. More research is needed to determine whether cumulative severity is higher in commercially thinned forests in other large western US wildfires.

Giant sequoia groves, located on the western slope of the central and southern Sierra Nevada mountains in California, USA, have been experiencing regeneration failure for more than a century due to the exclusion of wildfires. Giant sequoias are serotinous conifers and have evolved a strong relationship between high-severity fire and reproduction. While this relationship is widely recognized, only one previous peer-reviewed study has directly investigated giant sequoia reproduction and fire severity, and that study used different fires for each severity class. We conducted a study of giant sequoia reproduction and fire severity in a single fire, the KNP Complex fire of 2021, within the Redwood Mountain Grove in Sequoia and Kings Canyon National Park. We found that giant sequoia seedlings are more dominant relative to other conifer species and are growing faster in a large high-severity fire area than in adjacent low/moderate-severity areas. Distance to the nearest live sequoia seed source was not a significant factor in sequoia seedling density. Our results call into question the basis for widespread plans and projects designed to prevent high-severity fires and should reevaluate moving forward with proposed tree planting activities in high-severity fire areas within giant sequoia groves.