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Dr. Maria Notarnicola
Laboratory of Nutritional Biochemistry, National Institute of Gastroenterology “S. de Bellis” Research Hospital, 70013 Castellana Grotte (Ba), Italy;

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0 Cell Biology
0 Lipid Metabolism
0 nutrition biochemistry
0 Glucose and lipid metabolism
0 animal model of diseases

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Brief report
Published: 18 May 2021 in Diagnostics
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Alpha B-crystallin (CRYAB, HSPB5) belongs to the small heat shock protein (HSP) family and is highly expressed in various human cancers, suggesting a crucial role in tumor progression. However, few studies have examined CRYAB expression in colorectal cancer (CRC). In the present study, we investigated the relationship between CRYAB expression and the clinicopathological features of CRC samples. We comparatively analyzed CRYAB protein expression in 111 CRC tissues and normal adjacent colonic tissue, observing that it was significantly lower in CRC tissues than in corresponding non-cancerous tissues. Moreover, immunohistochemical analysis showed a significant correlation between CRYAB expression and high histological grade G3 (p = 0.033). In summary, our results point to its possible application as a prognostic biomarker in CRC patients.

ACS Style

Cristina Pagano; Giovanna Navarra; Patrizia Gazzerro; Mario Vitale; Maria Notarnicola; Maria Caruso; Elisabetta Cavalcanti; Raffaele Armentano; Chiara Laezza; Maurizio Bifulco. Association of Alpha B-Crystallin Expression with Tumor Differentiation Grade in Colorectal Cancer Patients. Diagnostics 2021, 11, 896 .

AMA Style

Cristina Pagano, Giovanna Navarra, Patrizia Gazzerro, Mario Vitale, Maria Notarnicola, Maria Caruso, Elisabetta Cavalcanti, Raffaele Armentano, Chiara Laezza, Maurizio Bifulco. Association of Alpha B-Crystallin Expression with Tumor Differentiation Grade in Colorectal Cancer Patients. Diagnostics. 2021; 11 (5):896.

Chicago/Turabian Style

Cristina Pagano; Giovanna Navarra; Patrizia Gazzerro; Mario Vitale; Maria Notarnicola; Maria Caruso; Elisabetta Cavalcanti; Raffaele Armentano; Chiara Laezza; Maurizio Bifulco. 2021. "Association of Alpha B-Crystallin Expression with Tumor Differentiation Grade in Colorectal Cancer Patients." Diagnostics 11, no. 5: 896.

Journal article
Published: 22 April 2021 in Viruses
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In order to provide insights into the evolutionary and epidemiological viral dynamics during the current COVID-19 pandemic in South Eastern Italy, a total of 298 genomes of SARS-CoV-2 strains collected in the Apulia and Basilicata regions, between March 2020 and January 2021, were sequenced. The genomic analysis performed on the draft genomes allowed us to assign the genetic clades and lineages of belonging to each sample and provide an overview of the main circulating viral variants. Our data showed the spread in Apulia and Basilicata of SARS-CoV-2 variants which have emerged during the second wave of infections and are being currently monitored worldwide for their increased transmission rate and their possible impact on vaccines and therapies. These results emphasize the importance of genome sequencing for the epidemiological surveillance of the new SARS-CoV-2 variants’ spread.

ACS Style

Loredana Capozzi; Angelica Bianco; Laura Del Sambro; Domenico Simone; Antonio Lippolis; Maria Notarnicola; Graziano Pesole; Lorenzo Pace; Domenico Galante; Antonio Parisi. Genomic Surveillance of Circulating SARS-CoV-2 in South East Italy: A One-Year Retrospective Genetic Study. Viruses 2021, 13, 731 .

AMA Style

Loredana Capozzi, Angelica Bianco, Laura Del Sambro, Domenico Simone, Antonio Lippolis, Maria Notarnicola, Graziano Pesole, Lorenzo Pace, Domenico Galante, Antonio Parisi. Genomic Surveillance of Circulating SARS-CoV-2 in South East Italy: A One-Year Retrospective Genetic Study. Viruses. 2021; 13 (5):731.

Chicago/Turabian Style

Loredana Capozzi; Angelica Bianco; Laura Del Sambro; Domenico Simone; Antonio Lippolis; Maria Notarnicola; Graziano Pesole; Lorenzo Pace; Domenico Galante; Antonio Parisi. 2021. "Genomic Surveillance of Circulating SARS-CoV-2 in South East Italy: A One-Year Retrospective Genetic Study." Viruses 13, no. 5: 731.

Review
Published: 09 April 2021 in International Journal of Molecular Sciences
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Nonalcoholic fatty liver disease (NAFLD) is considered a hepatic manifestation of metabolic syndrome, characterized from pathological changes in lipid and carbohydrate metabolism. Its main characteristics are excessive lipid accumulation and oxidative stress, which create a lipotoxic environment in hepatocytes leading to liver injury. Recently, many studies have focused on the identification of the genetic and epigenetic modifications that also contribute to NAFLD pathogenesis and their prognostic implications. The present review is aimed to discuss on cellular and metabolic alterations associated with NAFLD, which can be helpful to identify new noninvasive biomarkers. The identification of accumulated lipids in the cell membranes, as well as circulating cytokeratins and exosomes, provides new insights in understanding of NAFLD. This review also suggests that lifestyle modifications remain the main prevention and/or treatment for NAFLD.

ACS Style

Maria Notarnicola; Alberto Osella; Maria Caruso; Pasqua Pesole; Antonio Lippolis; Valeria Tutino; Caterina Bonfiglio; Valentina De Nunzio; Maria Scavo; Antonella Mirizzi; Isabella Franco; Tamara Lippolis; Rosalba D’Alessandro; Maria Refolo; Caterina Messa. Nonalcoholic Fatty Liver Disease: Focus on New Biomarkers and Lifestyle Interventions. International Journal of Molecular Sciences 2021, 22, 3899 .

AMA Style

Maria Notarnicola, Alberto Osella, Maria Caruso, Pasqua Pesole, Antonio Lippolis, Valeria Tutino, Caterina Bonfiglio, Valentina De Nunzio, Maria Scavo, Antonella Mirizzi, Isabella Franco, Tamara Lippolis, Rosalba D’Alessandro, Maria Refolo, Caterina Messa. Nonalcoholic Fatty Liver Disease: Focus on New Biomarkers and Lifestyle Interventions. International Journal of Molecular Sciences. 2021; 22 (8):3899.

Chicago/Turabian Style

Maria Notarnicola; Alberto Osella; Maria Caruso; Pasqua Pesole; Antonio Lippolis; Valeria Tutino; Caterina Bonfiglio; Valentina De Nunzio; Maria Scavo; Antonella Mirizzi; Isabella Franco; Tamara Lippolis; Rosalba D’Alessandro; Maria Refolo; Caterina Messa. 2021. "Nonalcoholic Fatty Liver Disease: Focus on New Biomarkers and Lifestyle Interventions." International Journal of Molecular Sciences 22, no. 8: 3899.

Journal article
Published: 28 March 2021 in International Journal of Molecular Sciences
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Functional alterations in irritable bowel syndrome have been associated with defects in bioenergetics and the mitochondrial network. Effects of high fat, adequate-protein, low carbohydrate ketogenic diet (KD) involve oxidative stress, inflammation, mitochondrial function, and biogenesis. The aim was to evaluate the KD efficacy in reducing the effects of stress on gut mitochondria. Newborn Wistar rats were exposed to maternal deprivation to induce IBS in adulthood. Intestinal inflammation (COX-2 and TRL-4); cellular redox status (SOD 1, SOD 2, PrxIII, mtDNA oxidatively modified purines); mitochondrial biogenesis (PPAR-γ, PGC-1α, COX-4, mtDNA content); and autophagy (Beclin-1, LC3 II) were evaluated in the colon of exposed rats fed with KD (IBD-KD) or standard diet (IBS-Std), and in unexposed controls (Ctrl). IBS-Std rats showed dysfunctional mitochondrial biogenesis (PPAR-γ, PGC-1α, COX-4, and mtDNA contents lower than in Ctrl) associated with inflammation and increased oxidative stress (higher levels of COX-2 and TLR-4, SOD 1, SOD 2, PrxIII, and oxidatively modified purines than in Ctrl). Loss of autophagy efficacy appeared from reduced levels of Beclin-1 and LC3 II. Feeding of animals with KD elicited compensatory mechanisms able to reduce inflammation, oxidative stress, restore mitochondrial function, and baseline autophagy, possibly via the upregulation of the PPAR-γ/PGC-1α axis.

ACS Style

Guglielmina Chimienti; Antonella Orlando; Angela Lezza; Benedetta D’Attoma; Maria Notarnicola; Isabella Gigante; Vito Pesce; Francesco Russo. The Ketogenic Diet Reduces the Harmful Effects of Stress on Gut Mitochondrial Biogenesis in a Rat Model of Irritable Bowel Syndrome. International Journal of Molecular Sciences 2021, 22, 3498 .

AMA Style

Guglielmina Chimienti, Antonella Orlando, Angela Lezza, Benedetta D’Attoma, Maria Notarnicola, Isabella Gigante, Vito Pesce, Francesco Russo. The Ketogenic Diet Reduces the Harmful Effects of Stress on Gut Mitochondrial Biogenesis in a Rat Model of Irritable Bowel Syndrome. International Journal of Molecular Sciences. 2021; 22 (7):3498.

Chicago/Turabian Style

Guglielmina Chimienti; Antonella Orlando; Angela Lezza; Benedetta D’Attoma; Maria Notarnicola; Isabella Gigante; Vito Pesce; Francesco Russo. 2021. "The Ketogenic Diet Reduces the Harmful Effects of Stress on Gut Mitochondrial Biogenesis in a Rat Model of Irritable Bowel Syndrome." International Journal of Molecular Sciences 22, no. 7: 3498.

Journal article
Published: 21 March 2021 in Nutrients
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Decreased serum vitamin D (VD) levels have been associated with gastrointestinal (GI) disorders, including irritable bowel syndrome (IBS). VD can also modulate the intestinal barrier. Given the link between the GI barrier’s alterations and diet, attention has aroused the positive effects of the Low FODMAP Diet (LFD) on IBS patients’ symptom profile. We evaluated the GI symptoms and the urinary and circulating markers of GI barrier function, the markers of inflammation and intestinal dysbiosis in 36 IBS patients with predominant diarrhea (IBS-D) (5 men and 31 women, 43.1 ± 1.7 years) categorized for their circulating VD levels in low (L-VD) and normal (N-VD) (cutoff = 20 ng/mL). Evaluations were performed before and after 12 weeks of LFD. At the baseline, L-VD patients showed a significantly worse symptom profile and altered small intestinal permeability (s-IP) than N-VD. After LFD, a significant increase in the circulating VD levels in both the subgroups and a significant improvement of s-IP in L-VD patients occurred. Finally, VD levels negatively correlated with the symptom score and fecal zonulin. These data highlight the close relationship between VD and the intestinal barrier and support their involvement in IBS-D pathophysiology. Moreover, the potentially positive role of LFD in the management of IBS-D was confirmed.

ACS Style

Michele Linsalata; Giuseppe Riezzo; Antonella Orlando; Benedetta D’Attoma; Laura Prospero; Valeria Tutino; Maria Notarnicola; Francesco Russo. The Relationship between Low Serum Vitamin D Levels and Altered Intestinal Barrier Function in Patients with IBS Diarrhoea Undergoing a Long-Term Low-FODMAP Diet: Novel Observations from a Clinical Trial. Nutrients 2021, 13, 1011 .

AMA Style

Michele Linsalata, Giuseppe Riezzo, Antonella Orlando, Benedetta D’Attoma, Laura Prospero, Valeria Tutino, Maria Notarnicola, Francesco Russo. The Relationship between Low Serum Vitamin D Levels and Altered Intestinal Barrier Function in Patients with IBS Diarrhoea Undergoing a Long-Term Low-FODMAP Diet: Novel Observations from a Clinical Trial. Nutrients. 2021; 13 (3):1011.

Chicago/Turabian Style

Michele Linsalata; Giuseppe Riezzo; Antonella Orlando; Benedetta D’Attoma; Laura Prospero; Valeria Tutino; Maria Notarnicola; Francesco Russo. 2021. "The Relationship between Low Serum Vitamin D Levels and Altered Intestinal Barrier Function in Patients with IBS Diarrhoea Undergoing a Long-Term Low-FODMAP Diet: Novel Observations from a Clinical Trial." Nutrients 13, no. 3: 1011.

Journal article
Published: 12 March 2021 in International Journal of Molecular Sciences
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The administration of a ketogenic diet (KD) has been considered therapeutic in subjects with irritable bowel syndrome (IBS). This study aimed to investigate the molecular mechanisms by which a low-carbohydrate diet, such as KD, can improve gastrointestinal symptoms and functions in an animal model of IBS by evaluating possible changes in intestinal tissue expression of endocannabinoid receptors. In rats fed a KD, we detected a significant restoration of cell damage to the intestinal crypt base, a histological feature of IBS condition, and upregulation of CB1 and CB2 receptors. The diet also affected glucose metabolism and intestinal membrane permeability, with an overexpression of the glucose transporter GLUT1 and tight junction proteins in treated rats. The present data suggest that CB receptors represent one of the molecular pathways through which the KD works and support possible cannabinoid-mediated protection at the intestinal level in the IBS rats after dietary treatment.

ACS Style

Isabella Gigante; Valeria Tutino; Francesco Russo; Valentina De Nunzio; Sergio Coletta; Raffaele Armentano; Alberto Crovace; Maria Caruso; Antonella Orlando; Maria Notarnicola. Cannabinoid Receptors Overexpression in a Rat Model of Irritable Bowel Syndrome (IBS) after Treatment with a Ketogenic Diet. International Journal of Molecular Sciences 2021, 22, 2880 .

AMA Style

Isabella Gigante, Valeria Tutino, Francesco Russo, Valentina De Nunzio, Sergio Coletta, Raffaele Armentano, Alberto Crovace, Maria Caruso, Antonella Orlando, Maria Notarnicola. Cannabinoid Receptors Overexpression in a Rat Model of Irritable Bowel Syndrome (IBS) after Treatment with a Ketogenic Diet. International Journal of Molecular Sciences. 2021; 22 (6):2880.

Chicago/Turabian Style

Isabella Gigante; Valeria Tutino; Francesco Russo; Valentina De Nunzio; Sergio Coletta; Raffaele Armentano; Alberto Crovace; Maria Caruso; Antonella Orlando; Maria Notarnicola. 2021. "Cannabinoid Receptors Overexpression in a Rat Model of Irritable Bowel Syndrome (IBS) after Treatment with a Ketogenic Diet." International Journal of Molecular Sciences 22, no. 6: 2880.

Journal article
Published: 28 December 2020 in Nutrients
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Background: Non-Alcoholic Fatty Liver Disease (NAFLD) is the most common chronic liver disease worldwide, and lifestyle modification is the current standard treatment. The aim of the study was to estimate the effect of two different physical activity (PA) programs, a Low Glycemic Index Mediterranean Diet (LGIMD), and their combined effect on the NAFLD score as measured by FibroScan®. Methods: Moderate or severe NAFLD subjects (n = 144) were randomly assigned to six intervention arms during three months. Interventions arms were a control diet, LGIMD, aerobic activity program (PA1), combined activity program (PA2), and LGIMD plus PA1 or LGIMD plus PA2. The data were compared at baseline, at 45 days, and at 90 days. Analysis of variance was performed under the intention-to-treat principle. Results: There was a statistically significant reduction in the NAFLD score after 45 days of treatment in every working arm except for Arm 1 (control diet). After 90 days, the best results were shown by the intervention arms in which LGIMD was associated with PA: LGIMD plus PA1 (−61.56 95% C.I. −89.61, −33.50) and LGIMD plus PA2 (−38.15 95% C.I −64.53, −11.77). Conclusion: All treatments were effective to reduce NAFLD scores, but LGIMD plus PA1 was the most efficient.

ACS Style

Isabella Franco; Antonella Bianco; Antonella Mirizzi; Angelo Campanella; Caterina Bonfiglio; Paolo Sorino; Maria Notarnicola; Valeria Tutino; Raffaele Cozzolongo; Vito Giannuzzi; Laura R. Aballay; Claudia Buongiorno; Irene Bruno; Alberto R. Osella. Physical Activity and Low Glycemic Index Mediterranean Diet: Main and Modification Effects on NAFLD Score. Results from a Randomized Clinical Trial. Nutrients 2020, 13, 66 .

AMA Style

Isabella Franco, Antonella Bianco, Antonella Mirizzi, Angelo Campanella, Caterina Bonfiglio, Paolo Sorino, Maria Notarnicola, Valeria Tutino, Raffaele Cozzolongo, Vito Giannuzzi, Laura R. Aballay, Claudia Buongiorno, Irene Bruno, Alberto R. Osella. Physical Activity and Low Glycemic Index Mediterranean Diet: Main and Modification Effects on NAFLD Score. Results from a Randomized Clinical Trial. Nutrients. 2020; 13 (1):66.

Chicago/Turabian Style

Isabella Franco; Antonella Bianco; Antonella Mirizzi; Angelo Campanella; Caterina Bonfiglio; Paolo Sorino; Maria Notarnicola; Valeria Tutino; Raffaele Cozzolongo; Vito Giannuzzi; Laura R. Aballay; Claudia Buongiorno; Irene Bruno; Alberto R. Osella. 2020. "Physical Activity and Low Glycemic Index Mediterranean Diet: Main and Modification Effects on NAFLD Score. Results from a Randomized Clinical Trial." Nutrients 13, no. 1: 66.

Journal article
Published: 02 December 2020 in Endocrine, Metabolic & Immune Disorders - Drug Targets
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Objective: Polyphenols extracted by table grape have been demonstrated to decrease cell proliferation in vitro and to exert anti-atherosclerotic and antithrombotic activities, regulating cell functions. A grape polyphenolic profile is affected by climate as well as a grape cultivar. This study was aimed to characterize the berry skin polyphenolic composition, antioxidant activity and antiproliferative properties of two black grape cultivars, Autumn Royal and Egnatia. Methods: The phenolic composition of Grape Skin Extracts (GSEs) was determined by HPLC analyses. The antioxidant activity was determined using DPPH, ABTS and ORAC tests. Caco2, HT29 and SW480 human colon cancer cell lines were used to test the effects of GSEs in vitro. Cell proliferation and cell cycle were assessed with the MTT method and a Muse cell analyzer, respectively. qPCR and Western Blotting analysis were used to evaluate gene and protein expression, respectively. Results: The total polyphenolic content and the total antioxidant capacity were significantly higher in Autumn Royal than in Egnatia. However, table grape Egnatia showed greater ability to affect cell proliferation and apoptosis, as well as to exert a growth arrest in the S phase of the cell cycle, particularly in the Caco2 cell line. Conclusion: These data suggest that the new grape variety Egnatia is an interesting source of phenolic compounds that could be of interest in the food and pharmaceutical industries.

ACS Style

Isabella Gigante; Rosa Anna Milella; Valeria Tutino; Giambattista DeBiase; Luciano Notarangelo; Maria Angela Giannandrea; Valentina De Nunzio; Antonella Orlando; Rosalba D’Alessandro; Maria Gabriella Caruso; Maria Notarnicola. Autumn Royal and Egnatia Grape Extracts Differently Modulate Cell Proliferation in Human Colorectal Cancer Cells. Endocrine, Metabolic & Immune Disorders - Drug Targets 2020, 20, 1740 -1750.

AMA Style

Isabella Gigante, Rosa Anna Milella, Valeria Tutino, Giambattista DeBiase, Luciano Notarangelo, Maria Angela Giannandrea, Valentina De Nunzio, Antonella Orlando, Rosalba D’Alessandro, Maria Gabriella Caruso, Maria Notarnicola. Autumn Royal and Egnatia Grape Extracts Differently Modulate Cell Proliferation in Human Colorectal Cancer Cells. Endocrine, Metabolic & Immune Disorders - Drug Targets. 2020; 20 (10):1740-1750.

Chicago/Turabian Style

Isabella Gigante; Rosa Anna Milella; Valeria Tutino; Giambattista DeBiase; Luciano Notarangelo; Maria Angela Giannandrea; Valentina De Nunzio; Antonella Orlando; Rosalba D’Alessandro; Maria Gabriella Caruso; Maria Notarnicola. 2020. "Autumn Royal and Egnatia Grape Extracts Differently Modulate Cell Proliferation in Human Colorectal Cancer Cells." Endocrine, Metabolic & Immune Disorders - Drug Targets 20, no. 10: 1740-1750.

Review
Published: 24 July 2020 in Cancers
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Colorectal cancer (CRC) is the third most common cancer worldwide. There is a need for the early diagnosis of CRC for a better prognostic outcome. It is, therefore, crucial to understand the CRC pathogenesis in all its aspects. In many cases, one of the main causes of cancer-related deaths is the presence of metastases. In this context, an often overlooked aspect is the metastatic tropism, since CRC, like other cancers, is more prone to metastasize some organs rather than others. Beyond the liver and lung, and differently from other types of cancers, a not usual site of CRC metastases is the bone. However, it may assume a crucial role in the development and the outcome of the disease. Therefore, this review aims to discuss the complex relations between bone markers and CRC pathogenesis, suggesting the use of these molecules as potential targets for therapeutic purposes. Different osteogenic molecules, some of whom are growth factors and are implicated in the different osteogenic pathways, have been proved to also be involved in CRC progression. Some of them are oncogenes, while others oncosuppressors, and in a future perspective, some of them may represent new potential CRC biomarkers.

ACS Style

Isabella Gigante; Valeria Tutino; Valentina De Nunzio; Maria Notarnicola. Colorectal Cancer and Bone Tissue: Fantastic Relations and Where to Find Them. Cancers 2020, 12, 2029 .

AMA Style

Isabella Gigante, Valeria Tutino, Valentina De Nunzio, Maria Notarnicola. Colorectal Cancer and Bone Tissue: Fantastic Relations and Where to Find Them. Cancers. 2020; 12 (8):2029.

Chicago/Turabian Style

Isabella Gigante; Valeria Tutino; Valentina De Nunzio; Maria Notarnicola. 2020. "Colorectal Cancer and Bone Tissue: Fantastic Relations and Where to Find Them." Cancers 12, no. 8: 2029.

Journal article
Published: 23 July 2020 in Molecules
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Grapes contain many flavonoid and non-flavonoid compounds with anticancer effects. In this work we fully characterized the polyphenolic profile of two grape skin extracts (GSEs), Autumn Royal and Egnatia, and assessed their effects on Polyunsaturated Fatty Acid (PUFA) membrane levels of Caco2 and SW480 human colon cancer cell lines. Gene expression of 15-lipoxygenase-1 (15-LOX-1), and peroxisome proliferator-activated receptor gamma (PPAR-γ), as well as cell morphology, were evaluated. The polyphenolic composition was analyzed by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry (UHPLC/QTOF) analysis. PUFA levels were evaluated by gas chromatography, and gene expression levels of 15-LOX-1 and PPAR-γ were analyzed by real-time Polymerase Chain Reaction (PCR). Morphological cell changes caused by GSEs were identified by field emission scanning electron microscope (FE-SEM) and photomicrograph examination. We detected a different profile of flavonoid and non-flavonoid compounds in Autumn Royal and Egnatia GSEs. Cultured cells showed an increase of total PUFA levels mainly after treatment with Autumn Royal grape, and were richer in flavonoids when compared with the Egnatia variety. Both GSEs were able to affect 15-LOX-1 and PPAR-γ gene expression and cell morphology. Our results highlighted a new antitumor mechanism of GSEs that involves membrane PUFAs and their downstream pathways.

ACS Style

Valeria Tutino; Isabella Gigante; Rosa Anna Milella; Valentina De Nunzio; Riccardo Flamini; Mirko De Rosso; Maria Principia Scavo; Nicoletta DePalo; Elisabetta Fanizza; Maria Gabriella Caruso; Maria Notarnicola. Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines. Molecules 2020, 25, 3352 .

AMA Style

Valeria Tutino, Isabella Gigante, Rosa Anna Milella, Valentina De Nunzio, Riccardo Flamini, Mirko De Rosso, Maria Principia Scavo, Nicoletta DePalo, Elisabetta Fanizza, Maria Gabriella Caruso, Maria Notarnicola. Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines. Molecules. 2020; 25 (15):3352.

Chicago/Turabian Style

Valeria Tutino; Isabella Gigante; Rosa Anna Milella; Valentina De Nunzio; Riccardo Flamini; Mirko De Rosso; Maria Principia Scavo; Nicoletta DePalo; Elisabetta Fanizza; Maria Gabriella Caruso; Maria Notarnicola. 2020. "Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines." Molecules 25, no. 15: 3352.

Journal article
Published: 02 June 2020 in Nutrients
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Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs). In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant. Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients. Twenty IBS-D patients underwent a 90 day personalised LFD programme, and were regularly evaluated at scheduled visits. At the diet’s end, both IBS symptoms and anthropometric parameters were significantly improved. A significant decrease in prostaglandin E2 also accompanied these reductions. As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased. In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs. Lipidomic data may be insightful for unravelling the molecular mechanisms associated with IBS-D pathophysiology.

ACS Style

Antonella Orlando; Valeria Tutino; Maria Notarnicola; Giuseppe Riezzo; Michele Linsalata; Caterina Clemente; Laura Prospero; Manuela Martulli; Benedetta D’Attoma; Valentina De Nunzio; Francesco Russo. Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective. Nutrients 2020, 12, 1 .

AMA Style

Antonella Orlando, Valeria Tutino, Maria Notarnicola, Giuseppe Riezzo, Michele Linsalata, Caterina Clemente, Laura Prospero, Manuela Martulli, Benedetta D’Attoma, Valentina De Nunzio, Francesco Russo. Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective. Nutrients. 2020; 12 (6):1.

Chicago/Turabian Style

Antonella Orlando; Valeria Tutino; Maria Notarnicola; Giuseppe Riezzo; Michele Linsalata; Caterina Clemente; Laura Prospero; Manuela Martulli; Benedetta D’Attoma; Valentina De Nunzio; Francesco Russo. 2020. "Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective." Nutrients 12, no. 6: 1.

Journal article
Published: 04 March 2020 in Nutrients
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The polyphenolic compounds present in grape extracts have chemopreventive and anticancer properties. Here, we studied the ability of two grape skin extracts (GSEs), Autumn Royal and Egnatia, to influence the cell motility and membrane fluidity regulated by the enzyme Stearoyl-CoA desaturase-1 (SCD1) which increases with the cancer aggressiveness. Caco2 and SW480 human colon cancer cell lines were treated with increasing concentrations of GSEs to evaluate cell proliferation and motility. SCD1 levels were evaluated in both treated cell lines, by membrane lipidomic analysis conducted by gas chromatography. The expression levels of SCD1 and other factors involved in the reorganization of the cytoskeleton and focal adhesions were assessed by Real-time PCR, Western Blotting, and Immunofluorescence staining. High-performance liquid chromatography (HPLC) analyses were performed to determine the phenolic composition in the GSEs, finding them more expressed in Autumn Royal than in Egnatia. Both treatments reduced the levels of SCD1, phospho-Rac1/Cdc42/Rac1/Cdc42 ratio, Cofilin, Vimentin, and phospho-Paxillin especially in Caco2 compared to SW480, showing a different behavior of the two cell lines to these natural compounds. Our findings show that GSEs block the cell migration and membrane fluidity through a new mechanism of action involving structural cellular components.

ACS Style

Valeria Tutino; Isabella Gigante; Maria Principia Scavo; Maria Grazia Refolo; Valentina De Nunzio; Rosa Anna Milella; Maria Gabriella Caruso; Maria Notarnicola. Stearoyl-CoA Desaturase-1 Enzyme Inhibition by Grape Skin Extracts Affects Membrane Fluidity in Human Colon Cancer Cell Lines. Nutrients 2020, 12, 693 .

AMA Style

Valeria Tutino, Isabella Gigante, Maria Principia Scavo, Maria Grazia Refolo, Valentina De Nunzio, Rosa Anna Milella, Maria Gabriella Caruso, Maria Notarnicola. Stearoyl-CoA Desaturase-1 Enzyme Inhibition by Grape Skin Extracts Affects Membrane Fluidity in Human Colon Cancer Cell Lines. Nutrients. 2020; 12 (3):693.

Chicago/Turabian Style

Valeria Tutino; Isabella Gigante; Maria Principia Scavo; Maria Grazia Refolo; Valentina De Nunzio; Rosa Anna Milella; Maria Gabriella Caruso; Maria Notarnicola. 2020. "Stearoyl-CoA Desaturase-1 Enzyme Inhibition by Grape Skin Extracts Affects Membrane Fluidity in Human Colon Cancer Cell Lines." Nutrients 12, no. 3: 693.

Review
Published: 06 January 2020 in International Journal of Molecular Sciences
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Exosomes are membrane-bound extracellular vesicles (EVs) released by most cells, having a size ranging from 30 to 150 nm, and are involved in mechanisms of cell-cell communication in physiological and pathological tissues. Exosomes are engaged in the transport of biomolecules, such as lipids, proteins, messenger RNAs, and microRNA, and in signal transmission through the intercellular transfer of components. In the context of proteins and nucleic acids transported from exosomes, our interest is focused on the Frizzled proteins family and related messenger RNA. Exosomes can regenerate stem cell phenotypes and convert them into cancer stem cells by regulating the Wnt pathway receptor family, namely Frizzled proteins. In particular, for gastrointestinal cancers, the Frizzled protein involved in those mechanisms is Frizzled-10 (FZD-10). Currently, increasing attention is being devoted to the protein and lipid composition of exosomes interior and membranes, representing profound knowledge of specific exosomes composition fundamental for their application as new delivering drug tools for cancer therapy. This review intends to cover the most recent literature on the use of exosome vesicles for early diagnosis, follow-up, and the use of these physiological nanovectors as drug delivery systems for gastrointestinal cancer therapy.

ACS Style

Maria Principia Scavo; Nicoletta DePalo; Valeria Tutino; Valentina De Nunzio; Chiara Ingrosso; Federica Rizzi; Maria Notarnicola; Maria Lucia Curri; Gianluigi Giannelli. Exosomes for Diagnosis and Therapy in Gastrointestinal Cancers. International Journal of Molecular Sciences 2020, 21, 367 .

AMA Style

Maria Principia Scavo, Nicoletta DePalo, Valeria Tutino, Valentina De Nunzio, Chiara Ingrosso, Federica Rizzi, Maria Notarnicola, Maria Lucia Curri, Gianluigi Giannelli. Exosomes for Diagnosis and Therapy in Gastrointestinal Cancers. International Journal of Molecular Sciences. 2020; 21 (1):367.

Chicago/Turabian Style

Maria Principia Scavo; Nicoletta DePalo; Valeria Tutino; Valentina De Nunzio; Chiara Ingrosso; Federica Rizzi; Maria Notarnicola; Maria Lucia Curri; Gianluigi Giannelli. 2020. "Exosomes for Diagnosis and Therapy in Gastrointestinal Cancers." International Journal of Molecular Sciences 21, no. 1: 367.

Journal article
Published: 01 December 2019 in Clinical Nutrition
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Summary Objectives Mediterranean diet has several beneficial effects on health, but data regarding the association between Mediterranean diet and knee osteoarthritis (OA) are limited mainly to cross-sectional studies. We investigated whether higher Mediterranean diet adherence is prospectively associated with lower risk of radiographic OA (ROA), radiographic symptomatic knee OA (SxOA) and pain worsening in North American people at high risk or having knee OA. Methods Adherence to the Mediterranean diet was evaluated using a validated Mediterranean diet score (aMED), categorized in five categories (Q1 to Q5, higher values reflecting higher adherence to Mediterranean diet). Knee OA outcomes included incident (1) ROA, (2) SxOA, as the new onset of a combination of a painful knee and ROA, (3) knee pain worsening, i.e. a Western Ontario and McMaster Universities Osteoarthritis Index difference between baseline and each annual exam of ≥14%. Results 4330 subjects (mean age: 61.1 years; 58.0% females) were included. Based on a multivariable Poisson regression analysis, during a mean follow-up period of 4 years, participants who were more highly adherent to a Mediterranean diet (Q5) reported lower risk of pain worsening (relative risk, RR = 0.96; 95% CI: 0.91–0.999) compared to those in Q1. In 2994 people free from SxOA at baseline, higher adherence to a Mediterranean diet was associated with a lower risk for SxOA during follow-up by 9% (Q5 vs. Q1; RR = 0.91; 95% CI: 0.82–0.998). No significant associations emerged between aMED and incident ROA. Conclusion Higher adherence to Mediterranean diet is associated with a lower risk of pain worsening and symptomatic forms of knee OA.

ACS Style

Nicola Veronese; Ai Koyanagi; Brendon Stubbs; Cyrus Cooper; Giuseppe Guglielmi; Renè Rizzoli; Leonardo Punzi; Domenico Rogoli; Maria Gabriella Caruso; Ornella Rotolo; Maria Notarnicola; Nasser Al-Daghri; Lee Smith; Jean-Yves Reginster; Stefania Maggi. Mediterranean diet and knee osteoarthritis outcomes: A longitudinal cohort study. Clinical Nutrition 2019, 38, 2735 -2739.

AMA Style

Nicola Veronese, Ai Koyanagi, Brendon Stubbs, Cyrus Cooper, Giuseppe Guglielmi, Renè Rizzoli, Leonardo Punzi, Domenico Rogoli, Maria Gabriella Caruso, Ornella Rotolo, Maria Notarnicola, Nasser Al-Daghri, Lee Smith, Jean-Yves Reginster, Stefania Maggi. Mediterranean diet and knee osteoarthritis outcomes: A longitudinal cohort study. Clinical Nutrition. 2019; 38 (6):2735-2739.

Chicago/Turabian Style

Nicola Veronese; Ai Koyanagi; Brendon Stubbs; Cyrus Cooper; Giuseppe Guglielmi; Renè Rizzoli; Leonardo Punzi; Domenico Rogoli; Maria Gabriella Caruso; Ornella Rotolo; Maria Notarnicola; Nasser Al-Daghri; Lee Smith; Jean-Yves Reginster; Stefania Maggi. 2019. "Mediterranean diet and knee osteoarthritis outcomes: A longitudinal cohort study." Clinical Nutrition 38, no. 6: 2735-2739.

Journal article
Published: 12 November 2019 in Nutrients
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Background: The high prevalence of non-alcoholic fatty liver disease (NAFLD) observed in Western countries is due to the concurrent epidemics of overweight/obesity and associated metabolic complications, both recognized risk factors. A Western dietary pattern has been associated with weight gain and obesity, and more recently with NAFLD. Methods: This is a baseline cross-sectional analysis of 136 subjects (79 males) enrolled consecutively in the NUTRIATT (NUTRItion and Ac-TiviTy) study. Study subjects had moderate or severe NAFLD diagnosed by using Fibroscan-CAP. Food Frequency Questionnaire was used to obtain information about food intake. Statistical analysis included descriptive statistics and a multivariable logistic regression model. Results: The mean age was 49.58 (±10.18) with a mean BMI of 33.41 (±4.74). A significant inverse relationship was revealed between winter ice-cream intake and NAFLD severity (O.R. 0.65, 95% C.I. 0.95–0.99); chickpeas intake and NAFLD severity (O.R. 0.57, 95% C.I. 0.34–0.97), and not industrial aged-cheeses type (O.R. 0.85, 95% C.I. 0.74–0.98). A statistically significant positive association also emerged between rabbit meat (O.R. 1.23, 95% C.I. 1.01–1.49), industrial type aged cheeses (O.R. 1.17, 95% C.I. 1.01–1.35), milk-based desserts (no winter ice cream) (O.R. 1.11, 95% C.I. 1.01–1.21), fats (O.R. 1.12, 95% C.I. 1.01–1.25), and NAFLD severity. Conclusion: The fresh foods from non-intensive farming and high legume intake that characterize the Mediterranean diet would seem to be beneficial for patients with NAFLD.

ACS Style

Antonella Mirizzi; Isabella Franco; Carla Maria Leone; Caterina Bonfiglio; Raffaele Cozzolongo; Maria Notarnicola; Vito Giannuzzi; Valeria Tutino; Valentina De Nunzio; Irene Bruno; Claudia Buongiorno; Angelo Campanella; Valentina Deflorio; Annamaria Pascale; Filippo Procino; Paolo Sorino; Alberto Rubén Osella. Effects of Some Food Components on Non-Alcoholic Fatty Liver Disease Severity: Results from a Cross-Sectional Study. Nutrients 2019, 11, 2744 .

AMA Style

Antonella Mirizzi, Isabella Franco, Carla Maria Leone, Caterina Bonfiglio, Raffaele Cozzolongo, Maria Notarnicola, Vito Giannuzzi, Valeria Tutino, Valentina De Nunzio, Irene Bruno, Claudia Buongiorno, Angelo Campanella, Valentina Deflorio, Annamaria Pascale, Filippo Procino, Paolo Sorino, Alberto Rubén Osella. Effects of Some Food Components on Non-Alcoholic Fatty Liver Disease Severity: Results from a Cross-Sectional Study. Nutrients. 2019; 11 (11):2744.

Chicago/Turabian Style

Antonella Mirizzi; Isabella Franco; Carla Maria Leone; Caterina Bonfiglio; Raffaele Cozzolongo; Maria Notarnicola; Vito Giannuzzi; Valeria Tutino; Valentina De Nunzio; Irene Bruno; Claudia Buongiorno; Angelo Campanella; Valentina Deflorio; Annamaria Pascale; Filippo Procino; Paolo Sorino; Alberto Rubén Osella. 2019. "Effects of Some Food Components on Non-Alcoholic Fatty Liver Disease Severity: Results from a Cross-Sectional Study." Nutrients 11, no. 11: 2744.

Journal article
Published: 22 May 2019 in Cancers
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Changes in the regulation of endocannabinoid production, together with an altered expression of their receptors are hallmarks of cancer, including colorectal cancer (CRC). Although several studies have been conducted to understand the biological role of the CB1 receptor in cancer, little is known about its involvement in the metastatic process of CRC. The aim of this study was to investigate the possible link between CB1 receptor expression and the presence of metastasis in patients with CRC, investigating the main signaling pathways elicited downstream of CB1 receptor in colon cancer. Fifty-nine consecutive patients, with histologically proven colorectal cancer, were enrolled in the study, of which 30 patients with synchronous metastasis, at first diagnosis and 29 without metastasis. A low expression of CB1 receptor were detected in primary tumor tissue of CRC patients with metastasis and consequently, we observed an alteration of CB1 receptor downstream signaling. These signaling routes were also altered in intestinal normal mucosa, suggesting that, normal mucosa surrounding the tumor provides a realistic picture of the molecules involved in tissue malignant transformation. These observations contribute to the idea that drugs able to induce CB1 receptor expression can be helpful in order to set new anticancer therapeutic strategies.

ACS Style

Valeria Tutino; Maria Gabriella Caruso; Valentina De Nunzio; Dionigi Lorusso; Nicola Veronese; Isabella Gigante; Maria Notarnicola; Gianluigi Giannelli. Down-Regulation of Cannabinoid Type 1 (CB1) Receptor and its Downstream Signaling Pathways in Metastatic Colorectal Cancer. Cancers 2019, 11, 708 .

AMA Style

Valeria Tutino, Maria Gabriella Caruso, Valentina De Nunzio, Dionigi Lorusso, Nicola Veronese, Isabella Gigante, Maria Notarnicola, Gianluigi Giannelli. Down-Regulation of Cannabinoid Type 1 (CB1) Receptor and its Downstream Signaling Pathways in Metastatic Colorectal Cancer. Cancers. 2019; 11 (5):708.

Chicago/Turabian Style

Valeria Tutino; Maria Gabriella Caruso; Valentina De Nunzio; Dionigi Lorusso; Nicola Veronese; Isabella Gigante; Maria Notarnicola; Gianluigi Giannelli. 2019. "Down-Regulation of Cannabinoid Type 1 (CB1) Receptor and its Downstream Signaling Pathways in Metastatic Colorectal Cancer." Cancers 11, no. 5: 708.

Journal article
Published: 25 April 2019 in International Journal of Molecular Sciences
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Chronic inflammation increases the risk of developing certain types of cancer, such as colorectal cancer (CRC). The oxidative metabolism of polyunsaturated fatty acids (PUFAs) has a strong effect on colonic tumorigenesis and the levels of arachidonic acid (AA) and eicosapentaenoic acid (EPA) can contribute to the development of an inflammatory microenvironment. Aim of this study was to evaluate the possible differences in the AA/EPA ratio tissue levels between CRC patients with and without synchronous metastases. Moreover, the expression of the most important inflammatory enzymes and mediators, linked with the AA/EPA ratio, have been also assessed. Sixty-eight patients with CRC were enrolled in the study, of which 33 patients with synchronous metastasis. Fatty acid profile analysis in tissue samples was done to examine the levels of AA and EPA. High levels of the AA/EPA ratio were detected in tumor tissue of patients with metastatic CRC. Moreover, an increase of expression of the main enzymes and mediators involved in inflammation was also detected in the same samples. The lipidomic approach of inflammation allows to evaluate lipid homeostasis changes that occur in cancer and in its metastatic process, in order to identify new biomarkers to be introduced into clinical practice.

ACS Style

Valeria Tutino; Valentina De Nunzio; Maria Gabriella Caruso; Nicola Veronese; Dionigi Lorusso; Marta Di Masi; Maria Lucrezia Benedetto; Maria Notarnicola. Elevated AA/EPA Ratio Represents an Inflammatory Biomarker in Tumor Tissue of Metastatic Colorectal Cancer Patients. International Journal of Molecular Sciences 2019, 20, 2050 .

AMA Style

Valeria Tutino, Valentina De Nunzio, Maria Gabriella Caruso, Nicola Veronese, Dionigi Lorusso, Marta Di Masi, Maria Lucrezia Benedetto, Maria Notarnicola. Elevated AA/EPA Ratio Represents an Inflammatory Biomarker in Tumor Tissue of Metastatic Colorectal Cancer Patients. International Journal of Molecular Sciences. 2019; 20 (8):2050.

Chicago/Turabian Style

Valeria Tutino; Valentina De Nunzio; Maria Gabriella Caruso; Nicola Veronese; Dionigi Lorusso; Marta Di Masi; Maria Lucrezia Benedetto; Maria Notarnicola. 2019. "Elevated AA/EPA Ratio Represents an Inflammatory Biomarker in Tumor Tissue of Metastatic Colorectal Cancer Patients." International Journal of Molecular Sciences 20, no. 8: 2050.

Journal article
Published: 05 April 2019 in Nutrients
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Few studies assessed the associations between dietary vitamin K and depressive symptoms. We aimed to investigate the association between dietary vitamin K and depressive symptoms in a large cohort of North American People. In this cross-sectional analysis, 4,375 participants that were aged 45–79 years from the Osteoarthritis Initiative were included. Dietary vitamin K intake was collected through a semi-quantitative food frequency questionnaire and categorized in quartiles. Depressive symptoms were diagnosed using the 20-item Center for Epidemiologic Studies-Depression (CES-D) ≥ 16. To investigate the associations between vitamin K intake and depressive symptoms, logistic regression analysis were run, which adjusted for potential confounders. Overall, 437 (=10%) subjects had depressive symptoms. After adjusting for 11 confounders, people with the highest dietary vitamin K intake had lower odds of having depressive symptoms (OR = 0.58; 95%CI: 0.43–0.80). This effect was only present in people not taking vitamin D supplementation. In conclusion, higher dietary vitamin K intake was significantly associated with a lower presence of depressive symptoms, also after accounting for potential confounders. Future longitudinal research is required to explore the directionality of the association.

ACS Style

Francesco Bolzetta; Nicola Veronese; Brendon Stubbs; Marianna Noale; Alberto Vaona; Jacopo Demurtas; Stefano Celotto; Chiara Cacco; Alberto Cester; Maria Gabriella Caruso; Rosa Reddavide; Maria Notarnicola; Stefania Maggi; Ai Koyanagi; Michele Fornaro; Joseph Firth; Lee Smith; Marco Solmi. The Relationship between Dietary Vitamin K and Depressive Symptoms in Late Adulthood: A Cross-Sectional Analysis from a Large Cohort Study. Nutrients 2019, 11, 787 .

AMA Style

Francesco Bolzetta, Nicola Veronese, Brendon Stubbs, Marianna Noale, Alberto Vaona, Jacopo Demurtas, Stefano Celotto, Chiara Cacco, Alberto Cester, Maria Gabriella Caruso, Rosa Reddavide, Maria Notarnicola, Stefania Maggi, Ai Koyanagi, Michele Fornaro, Joseph Firth, Lee Smith, Marco Solmi. The Relationship between Dietary Vitamin K and Depressive Symptoms in Late Adulthood: A Cross-Sectional Analysis from a Large Cohort Study. Nutrients. 2019; 11 (4):787.

Chicago/Turabian Style

Francesco Bolzetta; Nicola Veronese; Brendon Stubbs; Marianna Noale; Alberto Vaona; Jacopo Demurtas; Stefano Celotto; Chiara Cacco; Alberto Cester; Maria Gabriella Caruso; Rosa Reddavide; Maria Notarnicola; Stefania Maggi; Ai Koyanagi; Michele Fornaro; Joseph Firth; Lee Smith; Marco Solmi. 2019. "The Relationship between Dietary Vitamin K and Depressive Symptoms in Late Adulthood: A Cross-Sectional Analysis from a Large Cohort Study." Nutrients 11, no. 4: 787.

Journal article
Published: 07 November 2018 in Nutrients
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Celiac disease (CD) is a chronic immune-mediated disorder, characterized by enhanced paracellular permeability across the intestinal epithelium. The complex system of intercellular junctions, including tight junctions (TJs) and adherens junctions (AJs), seals together the epithelial cells to form a continuous layer. The improvements in barrier integrity have been related to modifications in intercellular junction protein expression. Polyamines (spermidine, spermine, and putrescine) actively participate in the modulation of the AJ expression. Both in vitro and in vivo studies have demonstrated that also probiotics can promote the integrity and the function of the intestinal barrier. On these bases, the present work investigated the protective effects exerted by Lactobacillus rhamnosus GG (L.GG) against the pepsin-trypsin-digested gliadin (PTG)-induced enteropathy in jejunal tissue samples of Wistar rats. In particular, the probiotic effects have been evaluated on the intestinal mucosal architecture, polyamine metabolism and intercellular junction protein expression (ZO-1, Occludin, Claudin-1, β-catenin and E-cadherin). The results from this study indicate that L.GG protects the intestinal mucosa of rats from PTG-induced damage, by preventing the reduction of the expression of the intercellular junction proteins. Consequently, a role for L.GG in the therapeutic management of the gluten-related disorders in humans could be hypothesized.

ACS Style

Antonella Orlando; Michele Linsalata; Giusy Bianco; Maria Notarnicola; Benedetta D’Attoma; Maria Principia Scavo; Angela Tafaro; Francesco Russo. Lactobacillus rhamnosus GG Protects the Epithelial Barrier of Wistar Rats from the Pepsin-Trypsin-Digested Gliadin (PTG)-Induced Enteropathy. Nutrients 2018, 10, 1698 .

AMA Style

Antonella Orlando, Michele Linsalata, Giusy Bianco, Maria Notarnicola, Benedetta D’Attoma, Maria Principia Scavo, Angela Tafaro, Francesco Russo. Lactobacillus rhamnosus GG Protects the Epithelial Barrier of Wistar Rats from the Pepsin-Trypsin-Digested Gliadin (PTG)-Induced Enteropathy. Nutrients. 2018; 10 (11):1698.

Chicago/Turabian Style

Antonella Orlando; Michele Linsalata; Giusy Bianco; Maria Notarnicola; Benedetta D’Attoma; Maria Principia Scavo; Angela Tafaro; Francesco Russo. 2018. "Lactobacillus rhamnosus GG Protects the Epithelial Barrier of Wistar Rats from the Pepsin-Trypsin-Digested Gliadin (PTG)-Induced Enteropathy." Nutrients 10, no. 11: 1698.

Comparative study
Published: 24 September 2018 in Liver International
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Background & Aims The use of Ultrasound Scan (US) in Non‐Alcoholic Fatty Liver Disease (NAFLD) screening overloads US waiting lists. We hypothesized and tested a hybrid two‐step method, consisting of applying a formula, to exclude subjects at low risk, before US. Methods The sample included 2970 males and females (937 with NAFLD) diagnosed by US. We selected 8 formulas: Fatty Liver Index (FLI), Hepatic Steatosis Index (HIS), Body Mass Index (BMI), Waist circumference (WC), Abdominal Volume Index (AVI), Waist to Height ratio (WHtR), Waist/Height0.5 (WHT.5R) and Body Roundness Index (BRI), and calculated their performance in the two‐step method evaluating percentage reduction of the number of liver US (US reduction percentage), percentage of false negative and percentage of NAFLD identified. Results The US reductions percentage were, 52.2% (WHtR), 52.1% (HIS), 51.8% (FLI), 50.8% (BRI), 50.7% (BMI and WHt_5R), 46.5% (WC) and 45.2% (AVI). The false negative percentage were 8.5% (WHtR), 7.9% (BRI), 7.3% (WHt_5R), 7.2% (BMI), 6.7% (HIS), 6.6% (FLI), 5.6% (WC) and 5.2% (AVI). The best percentage of NALFD identified was obtained using AVI (83.6%) before US, then WC (82.2%), FLI (79%), HIS (78.9%), BMI (77.3%), WHt_5R (76.9%), BRI (74.8%) and WHtR (73%). Conclusion The best formula to use in two‐step diagnostic NAFLD screening was AVI, which showed a low false negative rate and a higher percentage of identified NAFLD. Other studies evaluating the economic advantages of this screening method are warranted. This article is protected by copyright. All rights reserved.

ACS Style

Filippo Procino; Giovanni Misciagna; Nicola Veronese; Maria Gabriella Caruso; Marisa Chiloiro; Anna Maria Cisternino; Maria Notarnicola; Caterina Bonfiglio; Irene Bruno; Claudia Buongiorno; Angelo Campanella; Valentina Deflorio; Isabella Franco; Rocco Guerra; Carla Maria Leone; Antonella Mirizzi; Alessandro Nitti; Alberto Rubén Osella; MICOL GROUP. Reducing NAFLD-screening time: A comparative study of eight diagnostic methods offering an alternative to ultrasound scans. Liver International 2018, 39, 187 -196.

AMA Style

Filippo Procino, Giovanni Misciagna, Nicola Veronese, Maria Gabriella Caruso, Marisa Chiloiro, Anna Maria Cisternino, Maria Notarnicola, Caterina Bonfiglio, Irene Bruno, Claudia Buongiorno, Angelo Campanella, Valentina Deflorio, Isabella Franco, Rocco Guerra, Carla Maria Leone, Antonella Mirizzi, Alessandro Nitti, Alberto Rubén Osella, MICOL GROUP. Reducing NAFLD-screening time: A comparative study of eight diagnostic methods offering an alternative to ultrasound scans. Liver International. 2018; 39 (1):187-196.

Chicago/Turabian Style

Filippo Procino; Giovanni Misciagna; Nicola Veronese; Maria Gabriella Caruso; Marisa Chiloiro; Anna Maria Cisternino; Maria Notarnicola; Caterina Bonfiglio; Irene Bruno; Claudia Buongiorno; Angelo Campanella; Valentina Deflorio; Isabella Franco; Rocco Guerra; Carla Maria Leone; Antonella Mirizzi; Alessandro Nitti; Alberto Rubén Osella; MICOL GROUP. 2018. "Reducing NAFLD-screening time: A comparative study of eight diagnostic methods offering an alternative to ultrasound scans." Liver International 39, no. 1: 187-196.