The degree of sexual dimorphism expressed by human bones is of primary importance for the development of accurate methods for sex estimation. The objective of the present study was to investigate sex differences in shape and size of the mandible using geometric morphometric methods. The study also aimed to examine the impact of age on the sex classification ability of the size and shape of the mandible. Computed tomography images of 190 Bulgarians (98 males and 92 females) were used in the study. Polygonal surface models of the skulls were generated and used for digitizing 45 landmarks located on the mandible. The raw three-dimensional coordinates of the landmarks were processed via generalized Procrustes superimposition. The sex differences in mandibular size and shape were evaluated for statistical significance. Multivariate regression was applied for correction of the allometric effect. Principal component analysis, discriminant analysis, and canonical variate analysis were also used in the study. Mandibular size differed significantly between males and females and achieved a sex classification accuracy of 87%. The significance of the sex differences in mandibular shape depended on the type of shape variables used in the analysis. The shape variables provided different classification accuracy: 78% using the Procrustes coordinates and 53% using the regression residuals. The male and female mandibles differed significantly in size and shape, including the allometric component. Mandibular size is a more effective sex indicator than shape. Age has an ambiguous effect on the classification accuracy of the size and shape variables of the mandible.

The level of sexual dimorphism manifested by human bones is an important factor for development of effective sex estimation methods. The aim of the study was to investigate the sexual dimorphism in the size and shape of the viscerocranium using geometric morphometric techniques. It also aimed to explore the sex differences in distinct viscerocranial regions and to establish the most dimorphic region with regard to size and shape. Computed tomography images of 156 males and 184 females were used in the study. Three-dimensional coordinates of 31 landmarks were acquired. Five landmark configurations were constructed from the viscerocranium and its orbital, nasal, maxillary, and zygomatic region. Generalized Procrustes superimposition, principal component analysis, and discriminant analysis were applied to each configuration. The significance of the sex differences in size and shape was assessed and significant differences were found in all configurations. The highest accuracy was obtained from both shape and size of the whole viscerocranium. Based on size only, the highest accuracy was achieved by the nasal region. The accuracy based on shape was generally low for all configurations, but the highest result was attained by the orbital region. Hence, size is a better sex discriminator than shape.