Neem resin-derived CDs (NR-CDs) exhibited a quantum yield of 21%, allowing them for multiple applications in sensitive detection of Fe²⁺ ions, and also in the degradation of multiple dyes in the presence of NaBH_4.

Gold nanoclusters (AuNCs) are promising nanomaterials for ratiometric fluorescent probes due to their tunable fluorescence wavelengths dependent on size and structure, as well as their biocompatibility and resistance to photobleaching. By incorporating an additional fluorescence spectral peak, dual-emission AuNC-based fluorescent probes have been developed to enhance the signal output reproducibility. These probes can be fabricated by integrating various luminescent nanomaterials with AuNCs. This review focuses on the preparation methods and applications of ratiometric fluorescent probes derived from AuNCs and other fluorescent nanomaterials or fluorescent dyes for both in vitro and in vivo bioimaging of target analytes. Additionally, the review delves into the sensing mechanisms of AuNC-based ratiometric probes, their synthetic strategies, and the challenges encountered when using AuNCs for ratiometric bioimaging. Moreover, we explore the application of protein-stabilized AuNCs and thiolate-capped AuNC-based ratiometric fluorescent probes for biosensing and bioimaging. Two primary methods for assembling AuNCs and fluorophores into ratiometric fluorescent probes are discussed: triggered assembly and self-assembly. Finally, we address the challenges and issues associated with ratiometric bioimaging using AuNCs and propose future directions for further advancing AuNCs as ratiometric imaging agents.

In this study, we have used the one-pot polycondensation method to prepare novel 2D conjugated microporous polymers (Th-F-CMP) containing thiophene (Th) and fluorene (Fl) moieties through the Suzuki cross-coupling reaction. The thermogravimetric analysis (TGA) data revealed that Th-F-CMP (T_d10 = 418 °C, char yield: 53 wt%). Based on BET analyses, the Th-F-CMP sample displayed a BET specific surface area of 30 m² g⁻¹, and the pore size was 2.61 nm. Next, to show the effectiveness of our study, we utilized Th-F-CMP as a fluorescence probe for the selective detection of Fe³⁺ ions at neutral pH with a linear range from 2.0 to 25.0 nM (R² = 0.9349). Furthermore, the electrochemical experimental studies showed that the Th-F-CMP framework had a superior specific capacity of 84.7 F g⁻¹ at a current density of 0.5 A g⁻¹ and outstanding capacitance retention (88%) over 2000 cycles.