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To simultaneously detect two metabolites of Aspergillus flavus, namely, cyclopiazonic acid (CPA) and aflatoxin (AFT), an ultrasensitive monoclonal antibody (mAb) YTT-2 against CPA was developed and characterized, with sensitivity to CPA of 1.32 ng mL−1. Along with the previously homemade mAb 1C11 against AFT, two mAbs were used to develop time-resolved fluorescence immunoprobes or gold immunoprobes. We developed two multiple-analyte paper immunosensors including time-resolved fluorescent immunochromatographic assay (TRFICA) and gold immunochromatographic assay (GICA) for the simultaneous determination of CPA and AFT. The TRFICA showed limits of determination (LODs) of 0.21 and 0.004 ng mL−1, while the GICA showed LODs of 0.33 and 0.01 ng mL−1 for CPA and AFT, respectively. To validate the specificity of the two rapid immunoassays, rice, corn and peanut samples were spiked with different concentrations of CPA and AFT. The two methods showed satisfactory recoveries (76.39~90.82% for CPA and 84.60~94.45% for AFT) and coefficients of variation of 3.50~7.80% for CPA and 4.12~13.90% for AFT. The results indicated that the TRFICA could complete the test within 5 min and had lower LODs and linear ranges, compared with that of GICA. The method developed in this work can be widely applied to the rapid and quantitative simultaneous determination of multiple harmful metabolites in fungi for food safety and health care. Graphical abstract
Tingting Yan; Zhaowei Zhang; Qi Zhang; Xiaoqian Tang; Du Wang; Xiaofeng Hu; Wen Zhang; Xiaomei Chen; Peiwu Li. Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies. Microchimica Acta 2020, 187, 1 -8.
AMA StyleTingting Yan, Zhaowei Zhang, Qi Zhang, Xiaoqian Tang, Du Wang, Xiaofeng Hu, Wen Zhang, Xiaomei Chen, Peiwu Li. Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies. Microchimica Acta. 2020; 187 (12):1-8.
Chicago/Turabian StyleTingting Yan; Zhaowei Zhang; Qi Zhang; Xiaoqian Tang; Du Wang; Xiaofeng Hu; Wen Zhang; Xiaomei Chen; Peiwu Li. 2020. "Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies." Microchimica Acta 187, no. 12: 1-8.
Selenium is one of the most attractive nutrient elements for antioxidant, anticancer and immunomodulatory effects, which is obtained from the agricultural food and environment. Herein, a gold nanocages/fluorinated graphene (AuNCs/FG) nanocomposite modified glassy carbon electrode (GCE) was fabricated for the electrochemical determination of selenium by square wave anodic stripping voltammetry. Under the optimal parameters, a wide linear relationship between peak current of selenium on AuNCs/FG/GCE and selenium concentration was observed between 0.002-5.0 mg/L. The method exhibited a low detection limit of 0.27 μg/L. The proposed method was applied successfully for the determination of selenium in agricultural food (tea, rapeseed sprouts, peanut) and environmental (tap water and lake water) samples. The results showed that the average levels of selenium were 0.04-0.27 mg/kg and not detectable, in agricultural food and water, respectively. Therefore, this method has great potential for the monitor of selenium in food and environmental samples.
Zhao Tan; Wenqin Wu; Nanri Yin; Mingming Jia; Xiaomei Chen; Yizhen Bai; Huimin Wu; Zhaowei Zhang; Peiwu Li. Determination of selenium in food and environmental samples using a gold nanocages/fluorinated graphene nanocomposite modified electrode. Journal of Food Composition and Analysis 2020, 94, 103628 .
AMA StyleZhao Tan, Wenqin Wu, Nanri Yin, Mingming Jia, Xiaomei Chen, Yizhen Bai, Huimin Wu, Zhaowei Zhang, Peiwu Li. Determination of selenium in food and environmental samples using a gold nanocages/fluorinated graphene nanocomposite modified electrode. Journal of Food Composition and Analysis. 2020; 94 ():103628.
Chicago/Turabian StyleZhao Tan; Wenqin Wu; Nanri Yin; Mingming Jia; Xiaomei Chen; Yizhen Bai; Huimin Wu; Zhaowei Zhang; Peiwu Li. 2020. "Determination of selenium in food and environmental samples using a gold nanocages/fluorinated graphene nanocomposite modified electrode." Journal of Food Composition and Analysis 94, no. : 103628.
Summary Quantitative fluorescence immunoassay is a vital and convenient method in point-of-care (POC) testing for food safety and clinical healthcare. However, current marking materials, from organic luciferin to quantum dots, still lack the requirement of increasing need for multiple targets of interest because of their too broad emission. Here, a new label material, perovskite nanocrystals (Pe-NCs), with a narrower photoluminescence spectrum is reported, and a sensitive and quantitative immunoassay method named the perovskite nanocrystal fluorescence-linked immunosorbent assay is preliminarily demonstrated. The key point of realizing water-dispersed and stable Pe-NCs was addressed by functionalizing with hydroxyl groups. The fluorescent probes (perovskite antibody) were constructed via electrostatic adsorption with narrow full-width at half-maximum (∼18 nm), laying the foundation for future study of multi-target detection. Both indirect competitive immunity and sandwich immunoassay were successfully demonstrated. This work provides a possible label material for POC tests and may open up a promising avenue of next-generation immunoassay.
Yuhui Dong; Xiaoqian Tang; Zhaowei Zhang; Jizhong Song; Tianchao Niu; Dan Shan; Haibo Zeng. Perovskite Nanocrystal Fluorescence-Linked Immunosorbent Assay Methodology for Sensitive Point-of-Care Biological Test. Matter 2020, 3, 273 -286.
AMA StyleYuhui Dong, Xiaoqian Tang, Zhaowei Zhang, Jizhong Song, Tianchao Niu, Dan Shan, Haibo Zeng. Perovskite Nanocrystal Fluorescence-Linked Immunosorbent Assay Methodology for Sensitive Point-of-Care Biological Test. Matter. 2020; 3 (1):273-286.
Chicago/Turabian StyleYuhui Dong; Xiaoqian Tang; Zhaowei Zhang; Jizhong Song; Tianchao Niu; Dan Shan; Haibo Zeng. 2020. "Perovskite Nanocrystal Fluorescence-Linked Immunosorbent Assay Methodology for Sensitive Point-of-Care Biological Test." Matter 3, no. 1: 273-286.
Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety. Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection. Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader. To employ portable readers in food safety, Pt-catalysis has attracted increasing attention due to its portability and reliability. In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer. We synthesized and characterized [email protected] and [email protected] conjugated with a second antibody ([email protected]). A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins. [email protected] was used to catalyze the production of O2 from H2O2 in a sealed vessel. The pressure of O2 was then recorded by a handheld barometer. The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading. After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0.09 to 16.0 ng/mL were achieved. Recovery was recorded as 83.1%–112.0% along with satisfactory results regarding inner- and inter-assay precision (relative standard deviation, RSD < 6.4%). Little cross-reaction was observed. Additionally, the POCAD was validated by high-performance liquid chromatography (HPLC) by using peanut and corn samples. The portable POCAD exhibits strong potential for applications in the on-site detection of small toxic molecules to ensure food safety.
Weiqi Zhang; Wenqin Wu; Chong Cai; Xiaofeng Hu; Hui Li; Yizhen Bai; Zhaowei Zhang; Peiwu Li. A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products. Toxins 2020, 12, 158 .
AMA StyleWeiqi Zhang, Wenqin Wu, Chong Cai, Xiaofeng Hu, Hui Li, Yizhen Bai, Zhaowei Zhang, Peiwu Li. A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products. Toxins. 2020; 12 (3):158.
Chicago/Turabian StyleWeiqi Zhang; Wenqin Wu; Chong Cai; Xiaofeng Hu; Hui Li; Yizhen Bai; Zhaowei Zhang; Peiwu Li. 2020. "A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products." Toxins 12, no. 3: 158.
Diacetoxyscirpenol (DAS) is a type A trichothecene mycotoxin with low molecular weight, and with respect to its toxicity and the occurrence in food and feed, it is known as a potential risk for public and animal health. In the present study, first, a sensitive and specific monoclonal antibody (5E7) was developed. Then, the antibody was applied to develop a competitive-type pressure-dependent immunosensor (CTPDI). The [email protected] was synthesized and labeled with goat antimouse antibody ([email protected]). Finally, the concentration of DAS was negatively correlated with the pressure signal. In the presence of optimal conditions, matrix-matched calibration curves were plotted for wheat samples, in which an optimal IC50 value (half maximal inhibitory concentration) of 3.08 ng/g was achieved. The CTPDI was further applied to detect natural and blind wheat samples, and validation was carried out by liquid chromatography-tandem mass spectrometry. The results showed that CTPDI was highly appropriate and accurate for detection of DAS in wheat.
Xiaoqian Tang; Jing Wu; Wenqin Wu; Zhaowei Zhang; Weiqi Zhang; Qi Zhang; Wen Zhang; Xiaomei Chen; Peiwu Li. Competitive-Type Pressure-Dependent Immunosensor for Highly Sensitive Detection of Diacetoxyscirpenol in Wheat via Monoclonal Antibody. Analytical Chemistry 2020, 92, 3563 -3571.
AMA StyleXiaoqian Tang, Jing Wu, Wenqin Wu, Zhaowei Zhang, Weiqi Zhang, Qi Zhang, Wen Zhang, Xiaomei Chen, Peiwu Li. Competitive-Type Pressure-Dependent Immunosensor for Highly Sensitive Detection of Diacetoxyscirpenol in Wheat via Monoclonal Antibody. Analytical Chemistry. 2020; 92 (5):3563-3571.
Chicago/Turabian StyleXiaoqian Tang; Jing Wu; Wenqin Wu; Zhaowei Zhang; Weiqi Zhang; Qi Zhang; Wen Zhang; Xiaomei Chen; Peiwu Li. 2020. "Competitive-Type Pressure-Dependent Immunosensor for Highly Sensitive Detection of Diacetoxyscirpenol in Wheat via Monoclonal Antibody." Analytical Chemistry 92, no. 5: 3563-3571.
Deoxynivalenol (DON) is a secondary metabolite produced by Fusarium, which is a trichothecene mycotoxin. As the main mycotoxin with high toxicity, wheat, barley, corn and their products are susceptible to contamination of DON. Due to the stability of this mycotoxin, traditional methods for DON reduction often require a strong oxidant, high temperature and high pressure with more energy consumption. Therefore, exploring green, efficient and environmentally friendly ways to degrade or reduce DON is a meaningful and challenging issue. Herein, a dendritic-like α-Fe2O3 was successfully prepared using a facile hydrothermal synthesis method at 160 °C, which was systematically characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). It was found that dendritic-like α-Fe2O3 showed superior activity for the photocatalytic degradation of DON in aqueous solution under visible light irradiation (λ > 420 nm) and 90.3% DON (initial concentration of 4.0 μg/mL) could be reduced in 2 h. Most of all, the main possible intermediate products were proposed through high performance liquid chromatography-mass spectrometry (HPLC-MS) after the photocatalytic treatment. This work not only provides a green and promising way to mitigate mycotoxin contamination but also may present useful information for future studies.
Huiting Wang; Jin Mao; Zhaowei Zhang; Qi Zhang; Liangxiao Zhang; Peiwu Li; Wen Zhang. Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation. Toxins 2019, 11, 105 .
AMA StyleHuiting Wang, Jin Mao, Zhaowei Zhang, Qi Zhang, Liangxiao Zhang, Peiwu Li, Wen Zhang. Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation. Toxins. 2019; 11 (2):105.
Chicago/Turabian StyleHuiting Wang; Jin Mao; Zhaowei Zhang; Qi Zhang; Liangxiao Zhang; Peiwu Li; Wen Zhang. 2019. "Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation." Toxins 11, no. 2: 105.
Multi-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical properties. Herein, a lateral flow immunoassay via time-resolved fluorescence was developed for the rapid, on-site, simultaneous, and quantitative sensing aflatoxin B1 (AFB1), zearalenone (ZEA), and chlorothalonil (CTN) in maize and peanut. The sample preparation was optimized to a single step, combining the grinding and extraction. Under optimal conditions, the sensing method lowered the limits of detection (LOD) to 0.16, 0.52, and 1.21 µg/kg in maize and 0.18, 0.57, and 1.47 µg/kg in peanut with an analytical range of 0.48–20, 1.56–200, and 3.63–300 µg/kg for AFB1, ZEA and CTN, respectively. The protocol could be completed within 15 min, including sample preparation and lateral flow immunoassay. The recovery range was 83.24–110.80%. An excellent correlation was observed between this approach and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for mycotoxins and gas chromatography-tandem mass spectrometry (GC-MS/MS) for pesticide in maize and peanut. This work could be applied in on-site multi-class sensing for food safety.
Du Wang; Jianguo Zhu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Jun Jiang; Xiaomei Chen; Xuefang Wang; Peiwu Li. Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation. Toxins 2019, 11, 56 .
AMA StyleDu Wang, Jianguo Zhu, Zhaowei Zhang, Qi Zhang, Wen Zhang, Li Yu, Jun Jiang, Xiaomei Chen, Xuefang Wang, Peiwu Li. Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation. Toxins. 2019; 11 (1):56.
Chicago/Turabian StyleDu Wang; Jianguo Zhu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Jun Jiang; Xiaomei Chen; Xuefang Wang; Peiwu Li. 2019. "Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation." Toxins 11, no. 1: 56.
A method is described for the simultaneous voltammetric determination of the heavy metal ions cadmium(II), lead(II), mercury(II), zinc(II), and copper(II) using a glassy carbon electrode (GCE) modified with magnetite (Fe3O4) nanoparticles and fluorinated multiwalled carbon nanotubes (Fe3O4/F-MWCNTs). The Fe3O4/F-MWCNT composite was synthesized by a hydrothermal method and characterized by X-ray photoelectron spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy, elemental mapping, electrochemical impedance spectroscopy, and square wave stripping voltammetry. Under the optimum conditions, the electrode displays excellent response to the ions. Figures of merit for Cd(II), Pb(II), Hg(II), Zn(II), and Cu(II), respectively, include (a) high electrochemical sensitivity (29.88, 43.50, 120.86, 47.34 and 90.31 (μA μM−1 cm−2), (b) well separated peaks (at −0.70, −0.53, +0.37, −1.11 and + 0.01 V vs. Ag/AgCl); (c) low limits of detection (0.014, 0.0084, 0.0039, 0.012, and 0.0053 μM); and (d) wide linear ranges (0.048–30.0, 0.028–30.0, 0.013–32.5, 0.039–32.5, and 0.017–31.5 μM). The modified GCE displays satisfying selectivity in the presence of potentially interfering other metal ions, stability for 30 days, and reproducibility of electrodes (with a relative standard deviation between 1.2 and 4.8% for n = 6). The modified GCE was applied to the determination of several heavy metal ions in (spiked) water and rice samples, and the results agreed well with data obtained by atomic fluorescence spectrometry or inductively coupled plasma-mass spectrometry. The dramatic performance probably result from the semi-ionic C-F bond on F-MWCNTs surface with a strong negative charge, the good electrical conductivity of the F-MWCNTs and Fe3O4, the synergistic interaction between Fe3O4 and F-MWCNTs, and the nafion conductive membrane improving the stability of the modified layer and enhanced cation adsorption.
Wenqin Wu; Mingming Jia; Zhongzheng Wang; Wen Zhang; Qi Zhang; Guozhen Liu; Zhaowei Zhang; Peiwu Li. Simultaneous voltammetric determination of cadmium(II), lead(II), mercury(II), zinc(II), and copper(II) using a glassy carbon electrode modified with magnetite (Fe3O4) nanoparticles and fluorinated multiwalled carbon nanotubes. Microchimica Acta 2019, 186, 97 .
AMA StyleWenqin Wu, Mingming Jia, Zhongzheng Wang, Wen Zhang, Qi Zhang, Guozhen Liu, Zhaowei Zhang, Peiwu Li. Simultaneous voltammetric determination of cadmium(II), lead(II), mercury(II), zinc(II), and copper(II) using a glassy carbon electrode modified with magnetite (Fe3O4) nanoparticles and fluorinated multiwalled carbon nanotubes. Microchimica Acta. 2019; 186 (2):97.
Chicago/Turabian StyleWenqin Wu; Mingming Jia; Zhongzheng Wang; Wen Zhang; Qi Zhang; Guozhen Liu; Zhaowei Zhang; Peiwu Li. 2019. "Simultaneous voltammetric determination of cadmium(II), lead(II), mercury(II), zinc(II), and copper(II) using a glassy carbon electrode modified with magnetite (Fe3O4) nanoparticles and fluorinated multiwalled carbon nanotubes." Microchimica Acta 186, no. 2: 97.
Multiclass chemical contamination of food has aroused ever-increasing attention due to the increasingly common findings of the co-occurrence of multiclass contamination, such as mycotoxin (aflatoxin M1, AFM1) and illegal additives (melamine, MEL). In the present study, a rapid, ultra-sensitive detection paper was developed based on a unique bridge-antibody label to realize on-site simultaneous detection of AFM1 and MEL in milk. This detection paper used the bridge-antibody label on fluorescent particles (i.e., the fluorescent Eu nanoparticles were first conjugated with polyclonal antibodies and then with monoclonal antibodies). Dramatically enhanced sensitivity was recorded, probably due to the increase in immobilization of efficient monoclonal antibodies onto microspheres. Under optimal conditions, the lower limits of detection were 0.009 and 0.024 ng/mL for AFM1 and MEL in milk, respectively, in comparison with similar works. Moreover, the cut-off values were 0.4 and 150 ng/mL for AFM1 and MEL, respectively. The recoveries ranged from 88.7% to 105.0% for AFM1 and from 84.6% to 117.7% for MEL, with relative standard deviations (RSDs) of 0.5%–9.9% during the intraday and interday experiments. Comparison experiments conducted using the detection paper, HPLC and UPLC-MS/MS found excellent agreement in the simultaneous detection of AFM1 and MEL in milk. This proposed method can be extensively employed for simultaneous monitoring of multiclass chemical contaminants to ensure food safety.
Guanghua Li; Lin Xu; Wenqin Wu; Du Wang; Jun Jiang; Xiaomei Chen; Wen Zhang; Saranya Poapolathep; Amnart Poapolathep; Zhaowei Zhang; Qi Zhang; Peiwu Li. On-Site Ultrasensitive Detection Paper for Multiclass Chemical Contaminants via Universal Bridge-Antibody Labeling: Mycotoxin and Illegal Additives in Milk as an Example. Analytical Chemistry 2018, 91, 1968 -1973.
AMA StyleGuanghua Li, Lin Xu, Wenqin Wu, Du Wang, Jun Jiang, Xiaomei Chen, Wen Zhang, Saranya Poapolathep, Amnart Poapolathep, Zhaowei Zhang, Qi Zhang, Peiwu Li. On-Site Ultrasensitive Detection Paper for Multiclass Chemical Contaminants via Universal Bridge-Antibody Labeling: Mycotoxin and Illegal Additives in Milk as an Example. Analytical Chemistry. 2018; 91 (3):1968-1973.
Chicago/Turabian StyleGuanghua Li; Lin Xu; Wenqin Wu; Du Wang; Jun Jiang; Xiaomei Chen; Wen Zhang; Saranya Poapolathep; Amnart Poapolathep; Zhaowei Zhang; Qi Zhang; Peiwu Li. 2018. "On-Site Ultrasensitive Detection Paper for Multiclass Chemical Contaminants via Universal Bridge-Antibody Labeling: Mycotoxin and Illegal Additives in Milk as an Example." Analytical Chemistry 91, no. 3: 1968-1973.
We describe a new fluorescence turn-on sensor for homogeneous detection of aflatoxin B1 (AFB1), a potent low molecular weight mycotoxin. A key innovation is the binding-induced intramolecular interaction involving the following two sets of probes: (1) a gold nanoparticle (AuNP) immobilized with hundreds of assistant oligonucleotides (AO) and dozens of anti-AFB1 monoclonal antibodies (mAb), and (2) the AFB1-BSA antigen conjugated with fluorophore-labeled signal oligonucleotides (SO) that contained a short sequence complementary to AO. Specific binding of AFB1-BSA to the antibody brought the fluorophore very close to the surface of the AuNP through a stable intramolecular hybridization between AO and SO, resulting in efficient quenching of fluorescence. The improved fluorescence quenching substantially reduced the background, due to the binding-induced intramolecular hybridization, and improved the signal-to-background ratio by 390%. In the presence of AFB1 in a sample, competitive binding of AFB1 in the sample to the antibodies immobilized on the AuNP caused the release of the fluorophore-labeled AFB1-BSA from the AuNP, turning on fluorescence. A detection limit of 2.3 nM was achieved, which meets the requirement for AFB1 detection at regulatory levels. Analyses of rice samples using this assay showed recoveries of 86-102%. Incorporating appropriate antibody probes could extend the assay to the detection of other small molecules.
Lin Xu; Hongquan Zhang; Xiaowen Yan; Hanyong Peng; Zhixin Wang; Qi Zhang; Peiwu Li; Zhaowei Zhang; X. Chris Le. Binding-Induced DNA Dissociation Assay for Small Molecules: Sensing Aflatoxin B1. ACS Sensors 2018, 3, 2590 -2596.
AMA StyleLin Xu, Hongquan Zhang, Xiaowen Yan, Hanyong Peng, Zhixin Wang, Qi Zhang, Peiwu Li, Zhaowei Zhang, X. Chris Le. Binding-Induced DNA Dissociation Assay for Small Molecules: Sensing Aflatoxin B1. ACS Sensors. 2018; 3 (12):2590-2596.
Chicago/Turabian StyleLin Xu; Hongquan Zhang; Xiaowen Yan; Hanyong Peng; Zhixin Wang; Qi Zhang; Peiwu Li; Zhaowei Zhang; X. Chris Le. 2018. "Binding-Induced DNA Dissociation Assay for Small Molecules: Sensing Aflatoxin B1." ACS Sensors 3, no. 12: 2590-2596.
A reliable, selective and rapid multiclass method has been developed for the simultaneous determination of 55 antibacterial drug residues in shrimp muscle samples by ultra high performance liquid chromatography-tandem mass spectrometry. The investigated compounds comprise of eight different classes, namely fluoroquinolones, sulfonamides and synergistic agents, tetracyclines, macrolides, lincosamides, penicillins, nitroimidazole and amphenicols. A simple liquid extraction procedure was developed consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA), followed by a defatting step with n-hexane. Chromatographic conditions were optimized, obtaining a running time <10 min. Mean recoveries ranged from 74.3% to 113.3%. For precision test, relative standard deviations (RSD, %) were lower than 15.0% and 24.0% for repeatability and reproducibility, respectively. Limits of detection and quantification ranged from 1.0 to 5.0 ng/g and 3.0–10.0 ng/g, respectively. Finally, the method was applied to real samples and the results demonstrated that enrofloxacin, ciprofloxacin, pefloxacin and doxycycline were quantifiable in shrimp samples.
Saksit Susakate; Saranya Poapolathep; Chanat Chokejaroenrat; Phanwimol Tanhan; Jana Hajslova; Mario Giorgi; Kanista Saimek; Zhaowei Zhang; Amnart Poapolathep. Multiclass analysis of antimicrobial drugs in shrimp muscle by ultra high performance liquid chromatography-tandem mass spectrometry. Journal of Food and Drug Analysis 2018, 27, 118 -134.
AMA StyleSaksit Susakate, Saranya Poapolathep, Chanat Chokejaroenrat, Phanwimol Tanhan, Jana Hajslova, Mario Giorgi, Kanista Saimek, Zhaowei Zhang, Amnart Poapolathep. Multiclass analysis of antimicrobial drugs in shrimp muscle by ultra high performance liquid chromatography-tandem mass spectrometry. Journal of Food and Drug Analysis. 2018; 27 (1):118-134.
Chicago/Turabian StyleSaksit Susakate; Saranya Poapolathep; Chanat Chokejaroenrat; Phanwimol Tanhan; Jana Hajslova; Mario Giorgi; Kanista Saimek; Zhaowei Zhang; Amnart Poapolathep. 2018. "Multiclass analysis of antimicrobial drugs in shrimp muscle by ultra high performance liquid chromatography-tandem mass spectrometry." Journal of Food and Drug Analysis 27, no. 1: 118-134.
A double-label immunochromatographic based assay (DL-ICA) was developed to monitor zearalenone (ZEN) levels in cereals, based on Eu nanoparticles (EuNP). The DL-ICA exhibited excellent sensitivity, reliability and selectivity in real samples. It showed low limits of detection (0.21-0.25 μg/kg) and broad analytical ranges (up to 120 μg/kg). The total analytical time, including sample preparation and DL-ICA execution, was reduced by 15 min compared with HPLC. The recovery rates ranged from 95.0% to 118.4%, with relative standard deviations (RSD) <11.6%. Inter- and intra-day validations were assessed, recovery rates of 89.3%-106.9% and RSD of 2.3%-9.7% were obtained, suggesting considerable stability and reliability for the assay. An excellent correlation was observed between DL-ICA and a reference HPLC method (R = 0.9899). Compared to current immunoassays, the current DL-ICA is inexpensive, highly sensitive, and rapid. Therefore, DL-ICA constitutes a novel tool for monitoring mycotoxins in food and feed to ensure safety. This article is protected by copyright. All rights reserved.
Du Wang; Zhaowei Zhang; Qi Zhang; Zhongzheng Wang; Wen Zhang; Li Yu; Hui Li; Jun Jiang; Peiwu Li. Rapid and sensitive double-label based immunochromatographic assay for zearalenone detection in cereals. ELECTROPHORESIS 2018, 39, 2125 -2130.
AMA StyleDu Wang, Zhaowei Zhang, Qi Zhang, Zhongzheng Wang, Wen Zhang, Li Yu, Hui Li, Jun Jiang, Peiwu Li. Rapid and sensitive double-label based immunochromatographic assay for zearalenone detection in cereals. ELECTROPHORESIS. 2018; 39 (16):2125-2130.
Chicago/Turabian StyleDu Wang; Zhaowei Zhang; Qi Zhang; Zhongzheng Wang; Wen Zhang; Li Yu; Hui Li; Jun Jiang; Peiwu Li. 2018. "Rapid and sensitive double-label based immunochromatographic assay for zearalenone detection in cereals." ELECTROPHORESIS 39, no. 16: 2125-2130.
To ensure protein beverage safety and prevent illegal melamine use to artificially increase protein content, a rapid, onsite, ultrasensitive detection method for melamine must be developed because melamine is detrimental to human health and life. Herein, an ultrasensitive time-resolved fluorescence detection paper (TFDP) was developed to detect melamine in protein beverages within 15 min using a one-step sample preparation. The lower limits of detection were 0.89, 0.94, and 1.05 ng/mL, and the linear ranges were 2.67–150, 2.82–150, and 3.15–150 ng/mL (R2>0.982) for peanut, walnut, and coconut beverages, respectively. The recovery rates were 85.86–110.60% with a coefficient of variation <7.80% in the spiking experiment. A high specificity was observed in the interferent experiment. When detecting real protein beverage samples, the TFDP and ultra-performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) results were consistent. This method is a promising alternative for rapid, onsite detection of melamine in beverages.
Guanghua Li; Du Wang; Aijun Zhou; Yimin Sun; Qi Zhang; Amnart Poapolathep; Li Zhang; Zhiyong Fan; Zhaowei Zhang; Peiwu Li. Rapid, On-Site, Ultrasensitive Melamine Quantitation Method for Protein Beverages Using Time-Resolved Fluorescence Detection Paper. Journal of Agricultural and Food Chemistry 2018, 66, 5671 -5676.
AMA StyleGuanghua Li, Du Wang, Aijun Zhou, Yimin Sun, Qi Zhang, Amnart Poapolathep, Li Zhang, Zhiyong Fan, Zhaowei Zhang, Peiwu Li. Rapid, On-Site, Ultrasensitive Melamine Quantitation Method for Protein Beverages Using Time-Resolved Fluorescence Detection Paper. Journal of Agricultural and Food Chemistry. 2018; 66 (22):5671-5676.
Chicago/Turabian StyleGuanghua Li; Du Wang; Aijun Zhou; Yimin Sun; Qi Zhang; Amnart Poapolathep; Li Zhang; Zhiyong Fan; Zhaowei Zhang; Peiwu Li. 2018. "Rapid, On-Site, Ultrasensitive Melamine Quantitation Method for Protein Beverages Using Time-Resolved Fluorescence Detection Paper." Journal of Agricultural and Food Chemistry 66, no. 22: 5671-5676.
Multiple-mycotoxin contamination has been frequently found in the agro-food monitoring due to the coexistence of fungi. However, many determination methods focused on a single mycotoxin, highlighting the demand for on-site determination of multiple mycotoxins in a single run. We develop a multicolor-based immunochromatographic strip (ICS) for simultaneous determination of aflatoxin B1 (AFB1), zearalenone (ZEN) and T-2 toxin in maize- and cereal-based animal feeds. The nanoparticles with different colors are conjugated with three monoclonal antibodies, which serve as the immunoassay probes. The decrease in color intensity is observed by the naked eyes, providing simultaneous quantification of three mycotoxins. The visible limits of detection for AFB1, ZEN and T-2 are estimated to be 0.5, 2, and 30 ng/mL, respectively. The cut-off values are 1, 10, and 50 ng/mL, respectively. Considerable specificity and stability are found using real samples. The results are in excellent agreement with those from high-performance liquid chromatography/tandem mass spectrometry. The multi-color ICS boasts sensitive and rapid visual differentiation and simultaneous semi-quantification of aflatoxin B1, zearalenone and T-2 toxin in maize- and cereal-based feed samples within 20 min.
Lin Xu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Du Wang; Hui Li; Peiwu Li. An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay. Toxins 2018, 10, 87 .
AMA StyleLin Xu, Zhaowei Zhang, Qi Zhang, Wen Zhang, Li Yu, Du Wang, Hui Li, Peiwu Li. An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay. Toxins. 2018; 10 (2):87.
Chicago/Turabian StyleLin Xu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Du Wang; Hui Li; Peiwu Li. 2018. "An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay." Toxins 10, no. 2: 87.
Peiwu Li; Wen Zhang; Zhaowei Zhang; Qi Zhang; Zhi-Yuan Chen. Fundamentals of Hapten-protein Conjugate Synthesis to Obtain High-quality Antibodies for Analysis of Food and Environmental Contaminants. Current Organic Chemistry 2018, 21, 2606 -2611.
AMA StylePeiwu Li, Wen Zhang, Zhaowei Zhang, Qi Zhang, Zhi-Yuan Chen. Fundamentals of Hapten-protein Conjugate Synthesis to Obtain High-quality Antibodies for Analysis of Food and Environmental Contaminants. Current Organic Chemistry. 2018; 21 (26):2606-2611.
Chicago/Turabian StylePeiwu Li; Wen Zhang; Zhaowei Zhang; Qi Zhang; Zhi-Yuan Chen. 2018. "Fundamentals of Hapten-protein Conjugate Synthesis to Obtain High-quality Antibodies for Analysis of Food and Environmental Contaminants." Current Organic Chemistry 21, no. 26: 2606-2611.
Aflatoxins and zearalenone (ZEN) are highly common mycotoxins in maize and maize-based products. This study aimed to report a time-resolved fluorescence immunochromatographic assay (TRFICA) developed using two idiotypic nanobodies for rapid, quantitative, and simultaneous detection of aflatoxin B1 (AFB1) and ZEN in maize and its products. A novel Eu/Tb(III) nanosphere with enhanced fluorescence was prepared as a label and conjugated to anti-idiotypic nanobody (AIdnb) and monoclonal antibody (mAb). On the basis of nanosphere–antibody conjugation, two patterns of competitive time-resolved strip methods (AIdnb–TRFICA and mAb–TRFICA) were established and compared. The half inhibition concentration of AIdnb–TRFICA was 0.46 and 0.86 ng·mL–1 for AFB1 and ZEN, which was 18.3- and 20.3-fold more sensitive than that of mAb–TRFICA for AFB1 and ZEN, respectively. Under optimal conditions, AIdnb–TRFICA for dual mycotoxin was established and provided a quantitative relationship ranging from 0.13 to 4.54 ng·mL–1 for AFB1 and 0.20 to 2.77 ng·mL–1 for ZEN, with a detection limit of 0.05 and 0.07 ng·mL–1 in the buffer solution, respectively. AIdnb–TRFICA showed good recoveries (72.6%–106.6%) in samples and was applied to detect dual mycotoxin in maize samples with satisfying results. To the best of our knowledge, it is the first report about a time-resolved strip method based on AIdnbs for dual mycotoxin.
Xiaoqian Tang; Peiwu Li; Qi Zhang; Zhaowei Zhang; Wen Zhang; Jun Jiang. Time-Resolved Fluorescence Immunochromatographic Assay Developed Using Two Idiotypic Nanobodies for Rapid, Quantitative, and Simultaneous Detection of Aflatoxin and Zearalenone in Maize and Its Products. Analytical Chemistry 2017, 89, 11520 -11528.
AMA StyleXiaoqian Tang, Peiwu Li, Qi Zhang, Zhaowei Zhang, Wen Zhang, Jun Jiang. Time-Resolved Fluorescence Immunochromatographic Assay Developed Using Two Idiotypic Nanobodies for Rapid, Quantitative, and Simultaneous Detection of Aflatoxin and Zearalenone in Maize and Its Products. Analytical Chemistry. 2017; 89 (21):11520-11528.
Chicago/Turabian StyleXiaoqian Tang; Peiwu Li; Qi Zhang; Zhaowei Zhang; Wen Zhang; Jun Jiang. 2017. "Time-Resolved Fluorescence Immunochromatographic Assay Developed Using Two Idiotypic Nanobodies for Rapid, Quantitative, and Simultaneous Detection of Aflatoxin and Zearalenone in Maize and Its Products." Analytical Chemistry 89, no. 21: 11520-11528.
Xiaofeng Hu; Suiyan Ouyang; Peiwu Li; Qi Zhang; Xianfeng Ren; Huali Xie; Zhaowei Zhang. Microarray Methodologies for Pesticides and Other Toxins in Foods. Analysis of Food Toxins and Toxicants 2017, 571 -592.
AMA StyleXiaofeng Hu, Suiyan Ouyang, Peiwu Li, Qi Zhang, Xianfeng Ren, Huali Xie, Zhaowei Zhang. Microarray Methodologies for Pesticides and Other Toxins in Foods. Analysis of Food Toxins and Toxicants. 2017; ():571-592.
Chicago/Turabian StyleXiaofeng Hu; Suiyan Ouyang; Peiwu Li; Qi Zhang; Xianfeng Ren; Huali Xie; Zhaowei Zhang. 2017. "Microarray Methodologies for Pesticides and Other Toxins in Foods." Analysis of Food Toxins and Toxicants , no. : 571-592.
Research about antibody specificity spectra was conducted to develop single-specific antibodies or broad-specific antibodies. Aflatoxins, as one class of high-toxicity mycotoxins, were selected as the research targets to investigate the effect of the immunogen dose on antibody specificity spectra. For this aim, 16 monoclonal antibodies were induced by low or high doses of aflatoxin B1-BSA, and 34 monoclonal antibodies were induced by low or high doses of aflatoxin M1-BSA. The specificities of the antibodies induced, whether by aflatoxin B1 conjugate or aflatoxin M1 conjugate, indicated that the low dose of the immunogen induced a narrow spectrum of antibody specificity, while the high dose of the immunogen showed an advantage to form a broad spectrum of antibody specificity. Therefore, this report provides important information for the development of new antibodies against small molecules like aflatoxins.
Peiwu Li; Jing Wu; Li Zhang; Zhiyong Fan; Tingting Yu; Feng Jiang; Xiaoqian Tang; Zhaowei Zhang; Wen Zhang; Qi Zhang. Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin. Toxins 2017, 9, 172 .
AMA StylePeiwu Li, Jing Wu, Li Zhang, Zhiyong Fan, Tingting Yu, Feng Jiang, Xiaoqian Tang, Zhaowei Zhang, Wen Zhang, Qi Zhang. Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin. Toxins. 2017; 9 (5):172.
Chicago/Turabian StylePeiwu Li; Jing Wu; Li Zhang; Zhiyong Fan; Tingting Yu; Feng Jiang; Xiaoqian Tang; Zhaowei Zhang; Wen Zhang; Qi Zhang. 2017. "Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin." Toxins 9, no. 5: 172.
An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and a test strip for the determination of total aflatoxins (AFTs) in rice and peanuts. The monoclonal antibody against AFT (mAbAFT) was homemade and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAbAFT. Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1.4 pg/mL and 2.9 pg/mL in rice and peanut matrices, respectively. The put-in and put-out durations were within 10 min. The recoveries for AFT in rice and peanut sample matrices were recorded from 86.25% to 118.0%, with relative deviations (RSD) below 12%. The assay was further validated via the comparison between this QDNB strip and the conventional HPLC method using spiked samples. Thus, the design provided a potential alternative for on-site, ultra-sensitive, and quantitative sensing of AFT that could also be expanded to other chemical contaminants for food safety.
Suiyan Ouyang; Zhaowei Zhang; Ting He; Peiwu Li; Qi Zhang; Xiaomei Chen; Du Wang; Hui Li; Xiaoqian Tang; Wen Zhang. An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip. Toxins 2017, 9, 137 .
AMA StyleSuiyan Ouyang, Zhaowei Zhang, Ting He, Peiwu Li, Qi Zhang, Xiaomei Chen, Du Wang, Hui Li, Xiaoqian Tang, Wen Zhang. An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip. Toxins. 2017; 9 (4):137.
Chicago/Turabian StyleSuiyan Ouyang; Zhaowei Zhang; Ting He; Peiwu Li; Qi Zhang; Xiaomei Chen; Du Wang; Hui Li; Xiaoqian Tang; Wen Zhang. 2017. "An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip." Toxins 9, no. 4: 137.
Mycotoxin contamination threatens health and life of humans and animals throughout the food supply chains. Many of the mycotoxins have been proven to be carcinogens, teratogens and mutagens. The reliable and sensitive sensing methods are requested to monitor mycotoxin contamination. Advanced sensors based on antibodies or aptamers boast the advantages of high sensitivity and rapidity, and have been used in the mycotoxin sensing. These sensors are miniaturized, thereby lowering costs, and are applicable to high-throughput modes. In this work, the latest developments in sensing strategies for mycotoxin determination were critically discussed. Optical and electrochemical sensing modes were compared. The sensing methods for single mycotoxin or multiple mycotoxins in food samples were reviewed, along with the challenges and the future of antibody or aptamer-based sensors. This work might promote academic studies and industrial applications for mycotoxin sensing.
Lin Xu; Zhaowei Zhang; Qi Zhang; Peiwu Li. Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers. Toxins 2016, 8, 239 .
AMA StyleLin Xu, Zhaowei Zhang, Qi Zhang, Peiwu Li. Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers. Toxins. 2016; 8 (8):239.
Chicago/Turabian StyleLin Xu; Zhaowei Zhang; Qi Zhang; Peiwu Li. 2016. "Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers." Toxins 8, no. 8: 239.