Background: Undernutrition, including stunting, is the cause of almost 45% of all deaths among children under the age of five. It not only affects child growth but also has a long-term negative influence on cognitive and physical abilities. Timor-Leste has the highest prevalence of child stunting in Southeast Asia. Therefore, this study aimed to identify the prevalence of stunting and factors associated with it. Methods: This was a cross-sectional study conducted using the Demographic and Health Survey of 2016 for Timor-Leste. The prevalence of stunting among children under five years of age was examined, and bivariable and multivariable logistic regression analysis was conducted to identify the factors associated with stunting. Results: Among 4,581 children under five years of age, growth in nearly 40% was stunted. The majority of the mothers with stunted children were of age 20–30 years with about 33% having their first baby at ≤19 years of age. Compared to women of <145 cm of height, those of ≥145 cm height had lower likelihood of having a stunted child (OR: 0.62, 95% CI: [0.48–0.80], p < 0.001). It was also interesting to note that the risk of stunting was lower among female children than male children [OR: 0.75, 95% CI: (0.64–0.88), p < 0.001] in our adjusted model. Similarly, other factors such as wealth index, postnatal care visits, currently breastfeeding, age of the child, and size of the child at birth were also associated with stunting. Conclusion: The present findings indicate that child stunting in Timor-Leste is mainly associated with maternal and child sociodemographic status. Hence, it is crucial to identify the quality of services provided by health facilities, the involvement of health workers and volunteers, and the intention of mothers to use the health services in Timor-Leste.

The possibility of developing a severe coronavirus infectious (COVID-19) disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has increased, particularly in patients with hematological malignancies. These patients are more likely to produce less antibody protection due to the immunocompromised nature of the disease and the anticancer treatments. Therefore, the present systematic review intended to evaluate the seroconversion rate of COVID-19 vaccines in patients with hematological malignancies compared with healthy controls. A comprehensive systematic search was conducted in Medline via PubMed, EMBASE, and the World Health Organization COVID-19 Research Database, as well as other searches (i.e., reference list from article search and manual searches), from December 2020 to May 2022. The outcome of interest included estimating the seroconversion rates following COVID-19 vaccination in patients with hematological malignancies and comparing them with those in healthy controls. After two-step screening, the data were extracted and the summary measures were calculated using a random-effects model. A total of 39 articles regarding patients with hematological malignancies were included in the present review. After the first vaccine dose, these patients had considerably lower antibody response rates (37.0%) compared with healthy controls (74.5%). Following the second vaccine dose, the seroconversion rate in patients reached 66.8%, whereas it peaked at 97.9% in the healthy controls following complete immunization. Notably, the BNT162b2 and ChAdOx1 vaccine combination achieved the highest seropositivity rate of approximately 70%. Multiple myeloma, chronic lymphocytic leukemia, and lymphoma were the cancers of interest in most of the studies. The results of the present study highlighted the comparatively low seropositivity rates in patients with hematological malignancies, with substantial variations in rates across disease groups. The findings emphasize the possibility of additional booster doses for these individuals to enhance their immunity against SARS-CoV-2.

Schistosomiasis mekongi infection represents a public health concern in Laos and Cambodia. While both countries have made significant progress in disease control over the past few decades, eradication has not yet been achieved. Recently, several studies reported the application of loop-mediated isothermal amplification (LAMP) for detecting Schistosoma DNA in low-transmission settings. The objective of this study was to develop a LAMP assay for Schistosoma mekongi using a simple DNA extraction method. In particular, we evaluated the utility of the LAMP assay for detecting S. mekongi DNA in human stool and snail samples in endemic areas in Laos. We then used the LAMP assay results to develop a risk map for monitoring schistosomiasis mekongi and preventing epidemics. A total of 272 stool samples were collected from villagers on Khon Island in the southern part of Laos in 2016. DNA for LAMP assays was extracted via the hot-alkaline method. Following the Kato-Katz method, we determined that 0.4% (1/272) of the stool samples were positive for S. mekongi eggs, as opposed to 2.9% (8/272) for S. mekongi DNA based on the LAMP assays. Snail samples (n = 11,762) were annually collected along the riverside of Khon Island from 2016 to 2018. DNA was extracted from pooled snails as per the hot-alkaline method. The LAMP assay indicated that the prevalence of S. mekongi in snails was 0.26% in 2016, 0.08% in 2017, and less than 0.03% in 2018. Based on the LAMP assay results, a risk map for schistosomiasis with kernel density estimation was created, and the distribution of positive individuals and snails was consistent. In a subsequent survey of residents, schistosomiasis prevalence among villagers with latrines at home was lower than that among villagers without latrines. This is the first study to develop and evaluate a LAMP assay for S. mekongi detection in stools and snails. Our findings indicate that the LAMP assay is an effective method for monitoring pathogen prevalence and creating risk maps for schistosomiasis.

The prevalence of Schistosoma mekongi in humans in the Lao People’s Democratic Republic (Lao PDR) has been relatively well monitored and has decreased due to effective interventions such as preventative chemotherapy with mass drug administration of praziquantel and community awareness programs. However, the prevalence among potential domestic reservoir animals remains broadly unclear, except for a few villages in the endemic area. Therefore, we conducted S. mekongi surveys for the domestic animals that had contact with Mekong River water. We conducted a cross-sectional study of the domestic animals in the seven sentinel villages in the Khong and Mounlapamok Districts of Champasak Province in southern Lao PDR in 2018 by random sampling with a statistically reliable sample size. Stool samples of the five predominant domestic animal species, cattle (n = 160), pig (n = 154), buffalo (n = 149), dog (n = 143), and goat (n = 85), were collected and examined using parasitological FECT method and the LAMP technique. The microscopic analysis did not detect any eggs of S. mekongi in the stool samples of any animal species. However, S. mekongi DNA was detected by the LAMP test in dog stool samples (0.7%; 1/143). On the other hand, the prevalence of other helminths was quite high and heterogeneous among animal species and sentinel sites by the microscopic analysis. These findings suggested that an intervention for S. mekongi infection should focus solely on human populations. However, periodic surveillance for S. mekongi infection among dogs should be conducted to monitor a possible resurgence of S. mekongi infection in the domestic animal population.

Background: Malaria remains the leading cause of under-five morbidity and mortality in low- and middle-income countries. Sleeping under mosquito nets, especially insecticide-treated nets (ITNs), is one of the best ways to prevent malaria as they form a physical and chemical barrier against mosquitoes. Therefore, the present study aimed to assess not only mosquito net use, but also how environmental factors, specifically land surface temperature, contribute to malaria prevention among households with children under 5 years of age in Lao PDR. Methods: The most recent Multiple Indicator Cluster Survey datasets of Lao PDR 2017 and the Japan Aerospace Exploration Agency (JAXA) Public Health Monitoring and Analysis Platform (JPMAP) were used. Data from 51,948 households were used in the analysis. A bivariate logistic regression analysis was followed by a multivariate logistic regression analysis to determine the factors influencing mosquito net use with children under five. Results: In this study, 77.8% of all households with children under 5 years of age slept under mosquito nets. Of these, 80.5% were ITNs (Olyset, Permanent, and other brands). Multivariate logistic regression analysis revealed that mosquito net use was significantly associated with the land surface temperature, ethno-linguistic group (Lao-Tai, Mon-Khmer, Hmong-Mien, Chinese-Tibetan, and other), education level of the household head, and wealth index quintile. Conclusions: The analysis of the present study suggested measures to intensify the use of mosquito nets with an emphasis on ethnic minorities living in hot areas to bring Lao PDR closer to the day that mosquito-borne infections such as malaria can be eliminated.

Aspiration pneumonia is a prevalent condition, and understanding the risk factors associated with discontinuation of oral intake upon discharge is crucial. This study aimed to identify such factors, thereby providing valuable insights for optimizing the use of limited healthcare resources and enhancing patient and family care. In this scoping review, data were collected through ICHUSHI using the search formula “Pneumonia-Aspiration/Thesaurus or Aspiration Pneumonia/All) and (Prognosis/Thesaurus or Prognosis/All).” The inclusion criteria encompassed Japanese patients hospitalized for aspiration pneumonia, with a clear outcome focused on the availability of oral intake. The exclusion criteria included text unavailability, studies from foreign countries, and cases involving not hospitalized patients. The risk of bias for each study was assessed using the Newcastle-Ottawa scale. Using this search formula, 1,646 articles were initially identified, culminating in the inclusion of six articles for analysis. The investigation revealed five significant risk factors: social status (age and gender), nutritional status (body mass index, Controlling Nutritional Status score, serum albumin, Basal Energy Expenditure, and low body weight), physical swallowing function (ambulatory ability before admission, Food Intake LEVEL scale (FILS), admission origin, bedridden status, Penetration-Aspiration scale, presence of residual pharyngeal material, and Basal Index), pneumonia severity (A-DROP score, a classification tool incorporating age, dehydration, oxygen demand, impaired consciousness, and hypotension), and comorbidities (pneumonia, dementia, mental illness, malignancy, chronic lower respiratory tract involvement, and renal failure). This scoping review identified five key risk factors associated with oral intake discontinuation upon discharge in patients hospitalized for aspiration pneumonia, providing valuable evidence for future clinical practice.