This work studies Ni-based catalyst deactivation and regeneration processes in the presence of H₂S under a biogas tri-reforming process for hydrogen production, which is an energy vector of great interest. 25 ppm of hydrogen sulfide were continuously added to the system in order to provoke an observable catalyst deactivation, and once fully deactivated two different regeneration processes were studied: a self-regeneration and a regeneration by low temperature oxidation. For that purpose, several Ni-based catalysts and a bimetallic Rh-Ni catalyst supported on alumina modified with CeO₂ and ZrO₂ were used as well as a commercial Katalco 57-5 for comparison purposes. Ni/Ce-Al₂O₃ and Ni/Ce-Zr-Al₂O₃ catalysts almost recovered their initial activity. For these catalysts, after the regeneration under oxidative conditions at low temperature, the CO₂ conversions achieved—79.5% and 86.9%, respectively—were significantly higher than the ones obtained before sulfur poisoning—66.7% and 45.2%, respectively. This effect could be attributed to the support modification with CeO₂ and the higher selectivity achieved for the Reverse Water-Gas-Shift (rWGS) reaction after catalysts deactivation. As expected, the bimetallic Rh-Ni/Ce-Al₂O₃ catalyst showed higher resistance to deactivation and its sulfur poisoning seems to be reversible. In the case of the commercial and Ni/Zr-Al₂O₃ catalysts, they did not recover their activity.