Lithium–sulfur batteries (LSBs) are considered a promising candidate for next-generation energy storage devices due to the advantages of high theoretical specific capacity, abundant resources and being environmentally friendly. However, the severe shuttle effect of polysulfides causes the low utilization of active substances and rapid capacity fading, thus seriously limiting their practical application. The introduction of conductive polymer-based interlayers between cathodes and separators is considered to be an effective method to solve this problem because they can largely confine, anchor and convert the soluble polysulfides. In this review, the recent progress of conductive polymer-based interlayers used in LSBs is summarized, including free-standing conductive polymer-based interlayers, conductive polymer-based interlayer modified separators and conductive polymer-based interlayer modified sulfur electrodes. Furthermore, some suggestions on rational design and preparation of conductive polymer-based interlayers are put forward to highlight the future development of LSBs.