This study presents and validates the psychometric characteristics of a short form of the Critical Thinking Self-assessment Scale (CTSAS). The original CTSAS was composed of six subscales representing the six components of Facione’s conceptualisation of critical thinking. The CTSAS short form kept the same structures and reduced the number of items from 115 in the original version, to 60. The CTSAS short form was tested with a sample of 531 higher education students from five countries (Germany, Greece, Lithuania, Romania, and Portugal) enrolled in different disciplinary fields (Business Informatics, Teacher Education, English as a Foreign Language, Business and Economics, and Veterinary Medicine). The confirmatory analysis was used to test the new instrument reliability, internal consistency, and construct validity. Both the models that hypothesized the six factors to be correlated and to tap into a second-order factor representing the complex concept of critical thinking, had acceptable fit to the data. The instrument showed strong internal consistency (α = 0.969) and strong positive correlations between skills and between the skills and the overall scale (p < 0.05). Despite the unbalanced sex distribution in the population (close to 75% females), the instrument retained its factorial structure invariance across sexes. Therefore, the new instrument shows adequate goodness of fit and retained stability and reliability, and is proposed as a valid and reliable means to evaluate and monitor critical thinking in university students.

Most donkey and local horse breeds are vulnerable to extinction as mechanization of agriculture progress throughout the world. The present study analyzed the pedigree and herd records of the donkey Asinina de Miranda breed (RAM), identifying genealogical and human factors that may affect the breed genetic diversity in the future and suggesting suitable strategies to breed preservation, early on the conservation program. The breeding rate was very low, with a ratio of foaling/live animals of 0.23 (178/760). The estimated number of founders and ancestors contributing to the reference population was 128 and 121. The number of founder herds in the reference population was 64, with an effective number of founder herds for the reference population of 7.6. The mean age of herd owners was 65.50 ± 0.884 years, with a negative association among the herd size and owner's age (P<0.001). In contrast, the size of the herd and the ownership of a male were both positively associated (P<0.001) with the herd number of in-born foals. Both the owners' age and the herd location (RAM home region v. dispersal region) were negatively associated with the foaling number (P<0.001). The main identified risk factors were: low breeding rates; low number of males and their unequal contribution to the genetic pool; unequal contribution of the herds to genetic pool; and advanced age of herd owners.

Even though Critical Thinking (CT) is an important goal for higher education institutions and labour market professionals, studies dealing with employers’ perceptions on CT meaning and envisioning in the workplace are scarce. Intended to tackle this gap, the current study provides an overview of the need for and practical application of CT in the workplace from the employers’ views. Adopting a qualitative research methodology, five focus group interviews were carried out, enrolling 28 representatives of different Portuguese Non-Governmental Organizations (NGO), public and private companies, in four different professional fields: Biomedicine, STEM (Sciences, Technology, Engineering and Mathematics), Social Sciences, and Humanities. Using Facione’s framework for data analysis, results suggest that CT is broadly recognized by employers as a set of interdependent skills and dispositions that are needed in new graduates, presenting slight differences in their practical application across professional fields. This paper also discusses and outlines implications to support universities in the promotion of students’ CT skills and dispositions.

Hypospadias is rarely reported in dogs. In this study we pre-sent 2 novel cases of this disorder of sexual development and, in addition, a case of hereditary sex reversal in a female with an enlarged clitoris. The first case was a male Moscow watchdog with a normal karyotype (78,XY) and the presence of the SRY gene. In this dog, perineal hypospadias, bilateral inguinal cryptorchidism and testes were observed. The second case, representing the Cocker spaniel breed, had a small penis with a hypospadic orifice of the urethra, bilateral cryptorchidism, testis and a rudimentary gonad inside an ovarian bursa, a normal female karyotype (78,XX) and a lack of the SRY gene. This animal was classified as a compound sex reversal (78,XX, SRY-negative) with the hypospadias syndrome. The third case was a Cocker spaniel female with an enlarged clitoris and internally located ovotestes. Cytogenetic and molecular analyses revealed a normal female karyotype (78,XX) and a lack of the SRY gene, while histology of the gonads showed an ovotesticular structure. This case was classified as a typical hereditary sex reversal syndrome (78,XX, SRY-negative). Molecular studies were focused on coding sequences of the SRY gene (case 1) and 2 candidates for monogenic hypospadias, namely MAMLD1 (mastermind-like domain containing 1) and SRD5A2 (steroid-5-alpha-reductase, alpha polypeptide 2). Sequencing of the entire SRY gene, including 5′- and 3′-flanking regions, did not reveal any mutation. The entire coding sequence of MAMLD1 and SRD5A2 was analyzed in all the intersexes, as well as in 4 phenotypically normal control dogs (3 females and 1 male). In MAMLD1 2 SNPs, including 1 missense substitution in exon 1 (c.128A>G, Asp43Ser), were identified, whereas in SRD5A2 7 polymorphisms, including 1 missense SNP (c.358G>A, Ala120Thr), were found. None of the identified polymorphisms cosegregated with the intersexual phenotype, thus, we cannot confirm that hypospadias may be associated with polymorphism in the coding sequence of the studied genes.

This review intends to reveal the current status of the instructional practices used to enhance Critical Thinking (CrT), Clinical Reasoning and Clinical Judgement (CJ) skills and dispositions in Health Sciences Higher Education programmes. After a three-step filtering process, 28 empirical studies on the effectiveness of the instructional strategies were analysed, following PICOS methodology. The analysis tackled the type of strategy, methods and outcomes, the research design, and assessment tools used. Diverse instructional designs, different in length, were used with variable success when compared with traditional lecturing. Several limitations were found, such as insufficient information regarding the intervention design and the alignment between learning outcomes and the assessment instruments. Due to the variability in sample sizes and research designs, it is difficult to conclude on the effectiveness of particular instructional strategies. Researchers ought to recognize the concerns herein discussed when designing, implementing and assessing future interventions.