The white corpuscles nucleus segmentation from microscopic blood images is major steps to diagnose blood-related diseases. The perfect and speedy segmentation system assists the hematologists to identify the diseases and take appropriate decision for better treatment. Therefore, fully automated white corpuscles nucleus segmentation model using deep convolution neural network, is proposed in the present study. The proposed model uses the combination of ‘binary_cross_entropy’ and ‘adam’ for maintaining learning rate in each network weight. To validate the potential and capability of the above proposed solution, ALL-IDB2 dataset is used. The complete set of images is partitioned into training and testing set and tedious experimentations have been performed. The best performing model is selected and the obtained training and testing accuracy of best performing model is reported as 98.69 % and 99.02 %, respectively. The staging analysis of proposed model is evaluated using sensitivity, specificity, Jaccard index, dice coefficient, accuracy and structure similarity index. The capability of proposed model is compared with performance of the region-based contour and fuzzy-based level-set method for same set of images and concluded that proposed model method is more accurate and effective for clinical purpose.