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Dr. Giuseppe Mannino
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze, 90128 Palermo, Italy

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0 Biochemistry
0 Food Chemistry
0 Food Science
0 Molecular Biology
0 Biostimulants

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Review
Published: 30 July 2021 in Antioxidants
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Proanthocyanidins (PACs) are a class of polyphenolic compounds that are attracting considerable interest in the nutraceutical field due to their potential health benefits. However, knowledge about the chemistry, biosynthesis, and distribution of PACs is limited. This review summarizes the main chemical characteristics and biosynthetic pathways and the main analytical methods aimed at their identification and quantification in raw plant matrices. Furthermore, meta-analytic approaches were used to identify the main plant sources in which PACs were contained and to investigate their potential effect on human health. In particular, a cluster analysis identified PACs in 35 different plant families and 60 different plant parts normally consumed in the human diet. On the other hand, a literature search, coupled with forest plot analyses, highlighted how PACs can be actively involved in both local and systemic effects. Finally, the potential mechanisms of action through which PACs may impact human health were investigated, focusing on their systemic hypoglycemic and lipid-lowering effects and their local anti-inflammatory actions on the intestinal epithelium. Overall, this review may be considered a complete report in which chemical, biosynthetic, ecological, and pharmacological aspects of PACs are discussed.

ACS Style

Giuseppe Mannino; Giorgia Chinigò; Graziella Serio; Tullio Genova; Carla Gentile; Luca Munaron; Cinzia Margherita Bertea. Proanthocyanidins and Where to Find Them: A Meta-Analytic Approach to Investigate Their Chemistry, Biosynthesis, Distribution, and Effect on Human Health. Antioxidants 2021, 10, 1229 .

AMA Style

Giuseppe Mannino, Giorgia Chinigò, Graziella Serio, Tullio Genova, Carla Gentile, Luca Munaron, Cinzia Margherita Bertea. Proanthocyanidins and Where to Find Them: A Meta-Analytic Approach to Investigate Their Chemistry, Biosynthesis, Distribution, and Effect on Human Health. Antioxidants. 2021; 10 (8):1229.

Chicago/Turabian Style

Giuseppe Mannino; Giorgia Chinigò; Graziella Serio; Tullio Genova; Carla Gentile; Luca Munaron; Cinzia Margherita Bertea. 2021. "Proanthocyanidins and Where to Find Them: A Meta-Analytic Approach to Investigate Their Chemistry, Biosynthesis, Distribution, and Effect on Human Health." Antioxidants 10, no. 8: 1229.

Review
Published: 27 July 2021 in Plants
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An increasing need for a more sustainable agriculturally-productive system is required in order to preserve soil fertility and reduce soil biodiversity loss. Microbial biostimulants are innovative technologies able to ensure agricultural yield with high nutritional values, overcoming the negative effects derived from environmental changes. The aim of this review was to provide an overview on the research related to plant growth promoting microorganisms (PGPMs) used alone, in consortium, or in combination with organic matrices such as plant biostimulants (PBs). Moreover, the effectiveness and the role of microbial biostimulants as a biological tool to improve fruit quality and limit soil degradation is discussed. Finally, the increased use of these products requires the achievement of an accurate selection of beneficial microorganisms and consortia, and the ability to prepare for future agriculture challenges. Hence, the implementation of the microorganism positive list provided by EU (2019/1009), is desirable.

ACS Style

Adele Castiglione; Giuseppe Mannino; Valeria Contartese; Cinzia Bertea; Andrea Ertani. Microbial Biostimulants as Response to Modern Agriculture Needs: Composition, Role and Application of These Innovative Products. Plants 2021, 10, 1533 .

AMA Style

Adele Castiglione, Giuseppe Mannino, Valeria Contartese, Cinzia Bertea, Andrea Ertani. Microbial Biostimulants as Response to Modern Agriculture Needs: Composition, Role and Application of These Innovative Products. Plants. 2021; 10 (8):1533.

Chicago/Turabian Style

Adele Castiglione; Giuseppe Mannino; Valeria Contartese; Cinzia Bertea; Andrea Ertani. 2021. "Microbial Biostimulants as Response to Modern Agriculture Needs: Composition, Role and Application of These Innovative Products." Plants 10, no. 8: 1533.

Journal article
Published: 16 July 2021 in Molecules
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Background: G-quadruplex (G4) forming sequences are recurrent in telomeres and promoter regions of several protooncogenes. In normal cells, the transient arrangements of DNA in G-tetrads may regulate replication, transcription, and translation processes. Tumors are characterized by uncontrolled cell growth and tissue invasiveness and some of them are possibly mediated by gene expression involving G-quadruplexes. The stabilization of G-quadruplex sequences with small molecules is considered a promising strategy in anticancer targeted therapy. Methods: Molecular virtual screening allowed us identifying novel symmetric bifunctionalized naphtho[1,2-b:8,7-b’]dithiophene ligands as interesting candidates targeting h-Telo and c-MYC G-quadruplexes. A set of unexplored naphtho-dithiophene derivatives has been synthesized and biologically tested through in vitro antiproliferative assays and spectroscopic experiments in solution. Results: The analysis of biological and spectroscopic data highlighted noteworthy cytotoxic effects on HeLa cancer cell line (GI50 in the low μM range), but weak interactions with G-quadruplex c-MYC promoter. Conclusions: The new series of naphtho[1,2-b:8,7-b’]dithiophene derivatives, bearing the pharmacophoric assumptions necessary to stabilize G-quadruplexes, have been designed and successfully synthesized. The interesting antiproliferative results supported by computer aided rational approaches suggest that these studies are a significant starting point for a lead optimization process and the isolation of a more efficacious set of G-quadruplexes stabilizers.

ACS Style

Antonino Lauria; Gabriele La Monica; Alessio Terenzi; Giuseppe Mannino; Riccardo Bonsignore; Alessia Bono; Anna Almerico; Giampaolo Barone; Carla Gentile; Annamaria Martorana. Antiproliferative Properties and G-Quadruplex-Binding of Symmetrical Naphtho[1,2-b:8,7-b’]dithiophene Derivatives. Molecules 2021, 26, 4309 .

AMA Style

Antonino Lauria, Gabriele La Monica, Alessio Terenzi, Giuseppe Mannino, Riccardo Bonsignore, Alessia Bono, Anna Almerico, Giampaolo Barone, Carla Gentile, Annamaria Martorana. Antiproliferative Properties and G-Quadruplex-Binding of Symmetrical Naphtho[1,2-b:8,7-b’]dithiophene Derivatives. Molecules. 2021; 26 (14):4309.

Chicago/Turabian Style

Antonino Lauria; Gabriele La Monica; Alessio Terenzi; Giuseppe Mannino; Riccardo Bonsignore; Alessia Bono; Anna Almerico; Giampaolo Barone; Carla Gentile; Annamaria Martorana. 2021. "Antiproliferative Properties and G-Quadruplex-Binding of Symmetrical Naphtho[1,2-b:8,7-b’]dithiophene Derivatives." Molecules 26, no. 14: 4309.

Journal article
Published: 13 July 2021 in Plant Physiology and Biochemistry
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Recently, biostimulants have been used in sustainable agriculture as priming agents able to increase crop tolerance to abiotic stressors. Here, a soil application of GHI_16_VHL, a plant protein hydrolysate-based biostimulant, was tested for its capability to mitigate severe water stress effects on Capsicum annuum at flowering time. The biostimulant influence on plant physiological status was monitored upon stress and its relief, by measuring chlorophyll levels, stomatal density, stem water potential, leaf gas exchanges and plant growth. Moreover, leaf osmoregulation and oxidative stress levels were also evaluated by quantifying free proline, total non-structural carbohydrates (NSC), ROS-scavenging activity and H2O2 level. Although biostimulant-primed plants showed a quicker decrease of stem water potential with respect to untreated plants upon drought imposition, they recovered faster probably due to the higher leaf osmolyte accumulation, namely NSC during drought. Moreover, leaf gas exchange recovery was prompted in biostimulant-treated plants, which showed an incremented stomatal density and the same chlorophyll level of well-watered plants at the end of the recovery phase. Hydrogen peroxide level was significantly lower during stress and early recovery in biostimulant primed plants, probably due to the higher catalase activity in treated plants before drought or to the higher level of non-enzymatic antioxidant scavengers in primed stressed plants. Finally, the biostimulant priming increased aboveground relative growth rate and final fruit yield of stressed plants. Taken together, our data suggest that the biostimulant priming treatment promotes a faster and more efficient plant recovery after drought.

ACS Style

Chiara Agliassa; Giuseppe Mannino; Dario Molino; Silvia Cavalletto; Valeria Contartese; Cinzia Margherita Bertea; Francesca Secchi. A new protein hydrolysate-based biostimulant applied by fertigation promotes relief from drought stress in Capsicum annuum L. Plant Physiology and Biochemistry 2021, 166, 1076 -1086.

AMA Style

Chiara Agliassa, Giuseppe Mannino, Dario Molino, Silvia Cavalletto, Valeria Contartese, Cinzia Margherita Bertea, Francesca Secchi. A new protein hydrolysate-based biostimulant applied by fertigation promotes relief from drought stress in Capsicum annuum L. Plant Physiology and Biochemistry. 2021; 166 ():1076-1086.

Chicago/Turabian Style

Chiara Agliassa; Giuseppe Mannino; Dario Molino; Silvia Cavalletto; Valeria Contartese; Cinzia Margherita Bertea; Francesca Secchi. 2021. "A new protein hydrolysate-based biostimulant applied by fertigation promotes relief from drought stress in Capsicum annuum L." Plant Physiology and Biochemistry 166, no. : 1076-1086.

Journal article
Published: 17 June 2021 in Agriculture
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Water deficit is one of the most problematic stressors worldwide. In this context, the use of biostimulants represents an increasingly ecological practice aimed to improve crop tolerance and mitigate the negative effects on the productivity. Here, the effect derived from the foliar application of ERANTHIS®®, a biostimulant based on seaweed (Ascophyllum nodosum and Laminaria digitata) and yeast extracts, was tested on tomato plants grown under mild water-stress conditions. The potential stress mitigation action was evaluated by monitoring morphometric (fresh weight and dry matter content), physiological (stem water potential) and biochemical (ROS scavenger enzymes activity, proline, abscisic acid, hydrogen peroxide and photosynthetic pigment content) parameters closely related to the occurrence and response to stress at both flowering and fruit-set timing. In general, we observed that plants grown under drought conditions and treated with the biostimulant had a lower amount of ABA, and MDA and proline correlated to a lower activity of ROS scavenger enzymes compared to untreated plants. These data, together with the higher stem water potential and photosynthetic pigment levels recorded for the treated plants, suggest that ERANTHIS®® may mitigate water stress effects on tomato.

ACS Style

Cristina Campobenedetto; Chiara Agliassa; Giuseppe Mannino; Ivano Vigliante; Valeria Contartese; Francesca Secchi; Cinzia Bertea. A Biostimulant Based on Seaweed (Ascophyllum nodosum and Laminaria digitata) and Yeast Extracts Mitigates Water Stress Effects on Tomato (Solanum lycopersicum L.). Agriculture 2021, 11, 557 .

AMA Style

Cristina Campobenedetto, Chiara Agliassa, Giuseppe Mannino, Ivano Vigliante, Valeria Contartese, Francesca Secchi, Cinzia Bertea. A Biostimulant Based on Seaweed (Ascophyllum nodosum and Laminaria digitata) and Yeast Extracts Mitigates Water Stress Effects on Tomato (Solanum lycopersicum L.). Agriculture. 2021; 11 (6):557.

Chicago/Turabian Style

Cristina Campobenedetto; Chiara Agliassa; Giuseppe Mannino; Ivano Vigliante; Valeria Contartese; Francesca Secchi; Cinzia Bertea. 2021. "A Biostimulant Based on Seaweed (Ascophyllum nodosum and Laminaria digitata) and Yeast Extracts Mitigates Water Stress Effects on Tomato (Solanum lycopersicum L.)." Agriculture 11, no. 6: 557.

Review article
Published: 26 May 2021 in Drug Discovery Today
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Matching biological data sequences is one of the most interesting ways to discover new bioactive compounds. In particular, matching cell chemosensitivity with a protein expression profile can be a useful approach to predict the activity of compounds against definite biological targets. In this review, we discuss this correlation. First, we analyze case studies in which some known drugs, acting on known targets, show a good correlation between their antiproliferative activities and protein expression when a large panel of tumor cells is considered. Then, we highlight how the application of in silico methods based on the correlation between cell line chemosensitivity and gene/protein expression patterns might be a quick, cheap, and interesting approach to predict the biological activity of investigated molecules.

ACS Style

Antonino Lauria; Gabriele La Monica; Carla Gentile; Giuseppe Mannino; Annamaria Martorana; Daniele Peri. Identification of biological targets through the correlation between cell line chemosensitivity and protein expression pattern. Drug Discovery Today 2021, 1 .

AMA Style

Antonino Lauria, Gabriele La Monica, Carla Gentile, Giuseppe Mannino, Annamaria Martorana, Daniele Peri. Identification of biological targets through the correlation between cell line chemosensitivity and protein expression pattern. Drug Discovery Today. 2021; ():1.

Chicago/Turabian Style

Antonino Lauria; Gabriele La Monica; Carla Gentile; Giuseppe Mannino; Annamaria Martorana; Daniele Peri. 2021. "Identification of biological targets through the correlation between cell line chemosensitivity and protein expression pattern." Drug Discovery Today , no. : 1.

Journal article
Published: 02 April 2021 in International Journal of Molecular Sciences
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The cell division cycle 25 (Cdc25) protein family plays a crucial role in controlling cell proliferation, making it an excellent target for cancer therapy. In this work, a set of small molecules were identified as Cdc25 modulators by applying a mixed ligand-structure-based approach and taking advantage of the correlation between the chemosensitivity of selected structures and the protein expression pattern of the proposed target. In the first step of the in silico protocol, a set of molecules acting as Cdc25 inhibitors were identified through a new ligand-based protocol and the evaluation of a large database of molecular structures. Subsequently, induced-fit docking (IFD) studies allowed us to further reduce the number of compounds biologically screened. In vitro antiproliferative and enzymatic inhibition assays on the selected compounds led to the identification of new structurally heterogeneous inhibitors of Cdc25 proteins. Among them, J3955, the most active inhibitor, showed concentration-dependent antiproliferative activity against HepG2 cells, with GI50 in the low micromolar range. When J3955 was tested in cell-cycle perturbation experiments, it caused mitotic failure by G2/M-phase cell-cycle arrest. Finally, Western blotting analysis showed an increment of phosphorylated Cdk1 levels in cells exposed to J3955, indicating its specific influence in cellular pathways involving Cdc25 proteins.

ACS Style

Antonino Lauria; Annamaria Martorana; Gabriele La Monica; Salvatore Mannino; Giuseppe Mannino; Daniele Peri; Carla Gentile. In Silico Identification of Small Molecules as New Cdc25 Inhibitors through the Correlation between Chemosensitivity and Protein Expression Pattern. International Journal of Molecular Sciences 2021, 22, 3714 .

AMA Style

Antonino Lauria, Annamaria Martorana, Gabriele La Monica, Salvatore Mannino, Giuseppe Mannino, Daniele Peri, Carla Gentile. In Silico Identification of Small Molecules as New Cdc25 Inhibitors through the Correlation between Chemosensitivity and Protein Expression Pattern. International Journal of Molecular Sciences. 2021; 22 (7):3714.

Chicago/Turabian Style

Antonino Lauria; Annamaria Martorana; Gabriele La Monica; Salvatore Mannino; Giuseppe Mannino; Daniele Peri; Carla Gentile. 2021. "In Silico Identification of Small Molecules as New Cdc25 Inhibitors through the Correlation between Chemosensitivity and Protein Expression Pattern." International Journal of Molecular Sciences 22, no. 7: 3714.

Journal article
Published: 06 March 2021 in International Journal of Molecular Sciences
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Hypercholesterolemia is one of the major causes of cardiovascular disease, the risk of which is further increased if other forms of dyslipidemia occur. Current therapeutic strategies include changes in lifestyle coupled with drug administration. Statins represent the most common therapeutic approach, but they may be insufficient due to the onset of resistance mechanisms and side effects. Consequently, patients with mild hypercholesterolemia prefer the use of food supplements since these are perceived to be safer. Here, we investigate the phytochemical profile and cholesterol-lowering potential of Protium heptaphyllum gum resin extract (PHE). Chemical characterization via HPLC-APCI-HRMS2 and GC-FID/MS identified 13 compounds mainly belonging to ursane, oleanane, and tirucallane groups. Studies on human hepatocytes have revealed how PHE is able to reduce cholesterol production and regulate the expression of proteins involved in its metabolism. (HMGCR, PCSK9, LDLR, FXR, IDOL, and PPAR). Moreover, measuring the inhibitory activity of PHE against HMGR, moderate inhibition was recorded. Finally, molecular docking studies identified acidic tetra- and pentacyclic triterpenoids as the main compounds responsible for this action. In conclusion, our study demonstrates how PHE may be a useful alternative to contrast hypercholesterolemia, highlighting its potential as a sustainable multitarget natural extract for the nutraceutical industry that is rapidly gaining acceptance as a source of health-promoting compounds.

ACS Style

Giuseppe Mannino; Piera Iovino; Antonino Lauria; Tullio Genova; Alberto Asteggiano; Monica Notarbartolo; Alessandra Porcu; Graziella Serio; Giorgia Chinigò; Andrea Occhipinti; Andrea Capuzzo; Claudio Medana; Luca Munaron; Carla Gentile. Bioactive Triterpenes of Protium heptaphyllum Gum Resin Extract Display Cholesterol-Lowering Potential. International Journal of Molecular Sciences 2021, 22, 2664 .

AMA Style

Giuseppe Mannino, Piera Iovino, Antonino Lauria, Tullio Genova, Alberto Asteggiano, Monica Notarbartolo, Alessandra Porcu, Graziella Serio, Giorgia Chinigò, Andrea Occhipinti, Andrea Capuzzo, Claudio Medana, Luca Munaron, Carla Gentile. Bioactive Triterpenes of Protium heptaphyllum Gum Resin Extract Display Cholesterol-Lowering Potential. International Journal of Molecular Sciences. 2021; 22 (5):2664.

Chicago/Turabian Style

Giuseppe Mannino; Piera Iovino; Antonino Lauria; Tullio Genova; Alberto Asteggiano; Monica Notarbartolo; Alessandra Porcu; Graziella Serio; Giorgia Chinigò; Andrea Occhipinti; Andrea Capuzzo; Claudio Medana; Luca Munaron; Carla Gentile. 2021. "Bioactive Triterpenes of Protium heptaphyllum Gum Resin Extract Display Cholesterol-Lowering Potential." International Journal of Molecular Sciences 22, no. 5: 2664.

Review
Published: 05 March 2021 in Agriculture
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In the past century, plant biostimulants have been increasingly used in agriculture as innovative and sustainable practice. Plant biostimulants have been mainly investigated as potential agents able to mitigate abiotic stress. However, few information is available about their ability to influence fruit quality or change fruit phytochemical composition. In particular, very little is known about their effects on anthocyanin synthesis and accumulation. Due to the increasing demand of consumers for healthier foods with high nutraceutical values, this review tries to fill the gap between anthocyanin content and biostimulant application. Here, we elucidate the chemical structure, biosynthetic pathway, plant distribution, and physiological role of anthocyanins in plants. Moreover, we discuss the potential implications for human health derived from the consumption of foods rich in these molecules. Finally, we report on literature data concerning the changes in anthocyanin content and profile after the application of biostimulant products on the most common anthocyanin-containing foods.

ACS Style

Giuseppe Mannino; Carla Gentile; Andrea Ertani; Graziella Serio; Cinzia Bertea. Anthocyanins: Biosynthesis, Distribution, Ecological Role, and Use of Biostimulants to Increase Their Content in Plant Foods—A Review. Agriculture 2021, 11, 212 .

AMA Style

Giuseppe Mannino, Carla Gentile, Andrea Ertani, Graziella Serio, Cinzia Bertea. Anthocyanins: Biosynthesis, Distribution, Ecological Role, and Use of Biostimulants to Increase Their Content in Plant Foods—A Review. Agriculture. 2021; 11 (3):212.

Chicago/Turabian Style

Giuseppe Mannino; Carla Gentile; Andrea Ertani; Graziella Serio; Cinzia Bertea. 2021. "Anthocyanins: Biosynthesis, Distribution, Ecological Role, and Use of Biostimulants to Increase Their Content in Plant Foods—A Review." Agriculture 11, no. 3: 212.

Journal article
Published: 11 January 2021 in Scientific Reports
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Roots have important roles for plants to withstand adverse environmental conditions, including salt stress. Biostimulant application was shown to enhance plant resilience towards abiotic stresses. Here, we studied the effect of a tannin-based biostimulant on tomato (Solanum lycopersicum L.) grown under salt stress conditions. We investigated the related changes at both root architecture (via imaging and biometric analysis) and gene expression (RNA-Seq/qPCR) levels. Moreover, in order to identify the main compounds potentially involved in the observed effects, the chemical composition of the biostimulant was evaluated by UV/Vis and HPLC-ESI-Orbitrap analysis. Sixteen compounds, known to be involved in root development and having a potential antioxidant properties were identified. Significant increase of root weight (+ 24%) and length (+ 23%) was observed when the plants were grown under salt stress and treated with the biostimulant. Moreover, transcriptome analysis revealed that the application of the biostimulant upregulated 285 genes, most of which correlated to root development and salt stress tolerance. The 171 downregulated genes were mainly involved in nutrient uptake. These data demonstrated that the biostimulant is able not only to restore root growth in salty soils, but also to provide the adequate plant nourishment by regulating the expression of essential transcription factors and stress responsive genes.

ACS Style

Cristina Campobenedetto; Giuseppe Mannino; Jules Beekwilder; Valeria Contartese; Rumyana Karlova; Cinzia M. Bertea. The application of a biostimulant based on tannins affects root architecture and improves tolerance to salinity in tomato plants. Scientific Reports 2021, 11, 1 -15.

AMA Style

Cristina Campobenedetto, Giuseppe Mannino, Jules Beekwilder, Valeria Contartese, Rumyana Karlova, Cinzia M. Bertea. The application of a biostimulant based on tannins affects root architecture and improves tolerance to salinity in tomato plants. Scientific Reports. 2021; 11 (1):1-15.

Chicago/Turabian Style

Cristina Campobenedetto; Giuseppe Mannino; Jules Beekwilder; Valeria Contartese; Rumyana Karlova; Cinzia M. Bertea. 2021. "The application of a biostimulant based on tannins affects root architecture and improves tolerance to salinity in tomato plants." Scientific Reports 11, no. 1: 1-15.

Journal article
Published: 24 December 2020 in Foods
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In this work, the food quality of four international (Campas, Chaffey, Fino de Jete and White) and three local (Daniela, Torre1 and Torre2) cultivars of Cherimoya (Annona cherimola Mill) was investigated. With this aim, pomological traits, sensorial attributes, physiochemical parameters (pH, total soluble content and total acidity), nutritional composition (macro- and micro-nutrients) and nutraceutical values (bioactive compounds, radical scavenging and antioxidant properties) were evaluated. Among the seven observed cultivars, Fino de Jete was identified as the best, not only for its commercial attributes such as pomological traits and physiochemical values, but also for its nutritional composition. On the other hand, Chaffey and Daniela were the cultivars with the highest content of polyphenols, proanthocyanidins, and with the strongest antioxidant capacity. Concerning the two local ecotypes, Torre1 and Torre2, they displayed a balanced nutritional profile that, if combined with their discrete nutraceutical, physicochemical and pomological values, may result in a reassessment of their commercial impact. In conclusion, our data provide interesting information about the pomological, nutritional, and nutraceutical properties of cherimoya fruits. Our results, in addition to promoting the commercial impact of local cultivars, may increase the use of individual cultivars in breeding programs.

ACS Style

Carla Gentile; Giuseppe Mannino; Eristanna Palazzolo; Giuseppe Gianguzzi; Anna Perrone; Graziella Serio; Vittorio Farina. Pomological, Sensorial, Nutritional and Nutraceutical Profile of Seven Cultivars of Cherimoya (Annona cherimola Mill). Foods 2020, 10, 35 .

AMA Style

Carla Gentile, Giuseppe Mannino, Eristanna Palazzolo, Giuseppe Gianguzzi, Anna Perrone, Graziella Serio, Vittorio Farina. Pomological, Sensorial, Nutritional and Nutraceutical Profile of Seven Cultivars of Cherimoya (Annona cherimola Mill). Foods. 2020; 10 (1):35.

Chicago/Turabian Style

Carla Gentile; Giuseppe Mannino; Eristanna Palazzolo; Giuseppe Gianguzzi; Anna Perrone; Graziella Serio; Vittorio Farina. 2020. "Pomological, Sensorial, Nutritional and Nutraceutical Profile of Seven Cultivars of Cherimoya (Annona cherimola Mill)." Foods 10, no. 1: 35.

Journal article
Published: 12 December 2020 in Biomolecules
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Plant biostimulants are under investigation as innovative products to improve plant production and fruit quality, without resulting in environmental and food contaminations. Here, the effects of the application of Expando, a biostimulant based on seaweed and yeast extracts, on plant productivity, fruit ripening times, and fruit quality of Solanum lycopersicum var. Micro-Tom were evaluated. After biostimulant treatment, a two-week reduction of ripening times and a concomitant enhancement of the production percentage during the earliest ripening times, in terms of both fruit yield (+110%) and size (+85%), were observed. Concerning fruit quality, proximate analysis showed that tomatoes treated with the biostimulant had better nutritional composition compared to untreated samples, since both the quality of unsatured fatty acids (C16:3ω3: +328%; C18:2ω6: −23%) and micronutrients essential for human health (Fe: +14%; Cu: +21%; Zn: +24%) were increased. From a nutraceutical point of view, despite strong changes in bioactive compound profile not being observed, an increase of the antioxidant properties was recorded in fruits harvested by plants treated with the biostimulant (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS): +38%; 2,2-diphenyl-1-picrylhydrazyl (DPPH): +11%). In conclusion, the biostimulant application was able to reduce the ripening times and fruit size, while slightly increasing nutritional and nutraceutical values, leading to more marketable tomato fruits.

ACS Style

Giuseppe Mannino; Cristina Campobenedetto; Ivano Vigliante; Valeria Contartese; Carla Gentile; Cinzia M. Bertea. The Application of a Plant Biostimulant Based on Seaweed and Yeast Extract Improved Tomato Fruit Development and Quality. Biomolecules 2020, 10, 1662 .

AMA Style

Giuseppe Mannino, Cristina Campobenedetto, Ivano Vigliante, Valeria Contartese, Carla Gentile, Cinzia M. Bertea. The Application of a Plant Biostimulant Based on Seaweed and Yeast Extract Improved Tomato Fruit Development and Quality. Biomolecules. 2020; 10 (12):1662.

Chicago/Turabian Style

Giuseppe Mannino; Cristina Campobenedetto; Ivano Vigliante; Valeria Contartese; Carla Gentile; Cinzia M. Bertea. 2020. "The Application of a Plant Biostimulant Based on Seaweed and Yeast Extract Improved Tomato Fruit Development and Quality." Biomolecules 10, no. 12: 1662.

Journal article
Published: 02 October 2020 in Plants
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Soybean (Glycine max Merr.) is a worldwide important legume crop, whose growth and yield are negatively affected by heat stress at germination time. Here, we tested the role of a biostimulant based on lignin derivatives, plant-derived amino acids, and molybdenum in enhancing soybean heat stress tolerance when applied on seeds. After treatment with the biostimulant at 35 °C, the seed biometric parameters were positively influenced after 24 h, meanwhile, germination percentage was increased after 72 h (+10%). RNA-Seq analyses revealed a modulation of 879 genes (51 upregulated and 828 downregulated) in biostimulant-treated seeds as compared with the control, at 24 h after incubation at 35 °C. Surprisingly, more than 33% of upregulated genes encoded for ribosomal RNA (rRNA) methyltransferases and proteins involved in the ribosome assembly, acting in a specific protein network. Conversely, the downregulated genes were involved in stress response, hormone signaling, and primary metabolism. Finally, from a biochemical point of view, the dramatic H2O2 reduction 40%) correlated to a strong increase in non-protein thiols (+150%), suggested a lower oxidative stress level in biostimulant-treated seeds, at 24 h after incubation at 35 °C. Our results provide insights on the biostimulant mechanism of action and on its application for seed treatments to improve heat stress tolerance during germination.

ACS Style

Cristina Campobenedetto; Giuseppe Mannino; Chiara Agliassa; Alberto Acquadro; Valeria Contartese; Christian Garabello; Cinzia Margherita Bertea. Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean. Plants 2020, 9, 1308 .

AMA Style

Cristina Campobenedetto, Giuseppe Mannino, Chiara Agliassa, Alberto Acquadro, Valeria Contartese, Christian Garabello, Cinzia Margherita Bertea. Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean. Plants. 2020; 9 (10):1308.

Chicago/Turabian Style

Cristina Campobenedetto; Giuseppe Mannino; Chiara Agliassa; Alberto Acquadro; Valeria Contartese; Christian Garabello; Cinzia Margherita Bertea. 2020. "Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean." Plants 9, no. 10: 1308.

Original research article
Published: 17 June 2020 in Frontiers in Plant Science
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Seed enhancement technologies have the potential to improve germination and seedling growth under environmental stress. The effects of KIEM®, an innovative biostimulant based on lignin derivatives and containing plant-derived amino acids and molybdenum, were investigated on cucumber (Cucumis sativus L.) seed germination. To determine the metabolic targets of this product, biometric, transcriptional and biochemical analyses were carried out on both non-treated and KIEM®-treated seeds incubated for 24 and 48 h under standard (28°C) and heat stress (35°C) conditions. The application of the biostimulant as a seed treatment increased the percent germination (+6.54%) and fresh biomass (+13%) at 48 h, and decreased the content of H2O2 in treated seeds at 28°C (−70%) and at 35°C (−80%). These changes in biometric and biochemical properties were accompanied by changes in expression levels of the genes coding for ROS-producing (RBOH) and scavenging (SOD, CAT, GST) enzymes and their specific activity. In general, the treatment with KIEM® in heat-stress condition appeared to stimulate a higher accumulation of three scavenger gene transcripts: CuZnSOD (+1.78), MnSOD (+1.75), and CAT (+3.39), while the FeSOD isoform was dramatically downregulated (0.24). Moreover, the amount of non-protein thiols, important antioxidant molecules, was increased by the biostimulant after 48 h (+20%). Taken together these results suggest that KIEM® acts through mitigation of the effects of the oxidative stress. Moreover, after 48 h, the pre-sowing treatment with KIEM® increased the transcription levels (+1.5) and the activity of isocitrate lyase (+37%), a key enzyme of the glyoxylate cycle, suggesting a potential effect of this product in speeding up the germination process. Finally, the chemical characterization of KIEM® identified five essential and three non-essential amino acids, and others bioactive compounds, including five organic and inorganic acids that might be potentially involved in its activity. Based on these data, insights on the potential mechanism of action of the biostimulant, suggested that there are broader applications as a product able to increase seed tolerance to different abiotic stress typical of adverse environmental conditions.

ACS Style

Cristina Campobenedetto; Eric Grange; Giuseppe Mannino; Jeroen Van Arkel; Jules Beekwilder; Rumyana Karlova; Christian Garabello; Valeria Contartese; Cinzia M. Bertea. A Biostimulant Seed Treatment Improved Heat Stress Tolerance During Cucumber Seed Germination by Acting on the Antioxidant System and Glyoxylate Cycle. Frontiers in Plant Science 2020, 11, 1 .

AMA Style

Cristina Campobenedetto, Eric Grange, Giuseppe Mannino, Jeroen Van Arkel, Jules Beekwilder, Rumyana Karlova, Christian Garabello, Valeria Contartese, Cinzia M. Bertea. A Biostimulant Seed Treatment Improved Heat Stress Tolerance During Cucumber Seed Germination by Acting on the Antioxidant System and Glyoxylate Cycle. Frontiers in Plant Science. 2020; 11 ():1.

Chicago/Turabian Style

Cristina Campobenedetto; Eric Grange; Giuseppe Mannino; Jeroen Van Arkel; Jules Beekwilder; Rumyana Karlova; Christian Garabello; Valeria Contartese; Cinzia M. Bertea. 2020. "A Biostimulant Seed Treatment Improved Heat Stress Tolerance During Cucumber Seed Germination by Acting on the Antioxidant System and Glyoxylate Cycle." Frontiers in Plant Science 11, no. : 1.

Journal article
Published: 04 June 2020 in Molecules
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Annona cherimola (Cherimoya) and Annona atemoya (Atemoya) are tropical plants known for their edible fruit. Scientific data suggest that their leaves, used in traditional medicine in the form of teas or infusions without evidence of toxicity, contain several bioactive compounds. However, only Annona muricata among all the Annona species is currently used in the nutraceutical field, and its dried leaves are marketed for tea preparation. In this work, we explored the nutraceutical potential of Atemoya and Cherimoya leaves, by evaluating their chemical profile and functional properties. Phytochemical analyses showed large amounts of phenolic compounds, in particular proanthocyanidins, and identified 18 compounds, either flavonoids or alkaloids. Concerning biological activity, we found antioxidative properties correlated with polyphenols, and antiproliferative activity against HeLa and HepG2 cell lines correlated with alkaloids. The obtained results demonstrate the potential use of Annona cherimola leaves for the preparation of dietary supplements aimed to promote the physiological redox balance. Moreover, the varietal comparison suggests that two commercial cultivars (Campas and White) and the local Torre 1, better suit this purpose. On the other hand, among the studied cultivars, Campas and Torre 1 are also the richest in alkaloids and, in consideration of the anti-proliferative properties of their extracts, dietary supplements based on these cultivars might also have chemo-preventive effects.

ACS Style

Giuseppe Mannino; Carla Gentile; Alessandra Porcu; Chiara Agliassa; Fabio CaraDonna; Cinzia Margherita Bertea. Chemical Profile and Biological Activity of Cherimoya (Annona cherimola Mill.) and Atemoya (Annona atemoya) Leaves. Molecules 2020, 25, 2612 .

AMA Style

Giuseppe Mannino, Carla Gentile, Alessandra Porcu, Chiara Agliassa, Fabio CaraDonna, Cinzia Margherita Bertea. Chemical Profile and Biological Activity of Cherimoya (Annona cherimola Mill.) and Atemoya (Annona atemoya) Leaves. Molecules. 2020; 25 (11):2612.

Chicago/Turabian Style

Giuseppe Mannino; Carla Gentile; Alessandra Porcu; Chiara Agliassa; Fabio CaraDonna; Cinzia Margherita Bertea. 2020. "Chemical Profile and Biological Activity of Cherimoya (Annona cherimola Mill.) and Atemoya (Annona atemoya) Leaves." Molecules 25, no. 11: 2612.

Journal article
Published: 03 April 2020 in Nutrients
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Vaccinium macrocarpon (syn. American Cranberry) is employed in dietary supplements (DS) with the aim to improve urinary tract well-being. This property is linked to the antiadhesion-activity of proanthocyanidins (PACs) against uropathogenic-bacteria. However, the current European legislation has been criticized for being weak and ineffective. Indeed, recent scientific works report mislabeled, contaminated, and adulterated supplements containing dangerous or unknown compounds, or sold at toxic doses. In this work, we analysed 24 DS that claim to contain cranberry, and to have a specific dosage of PACs. Our tests included the control of the good manufacturing practice according to the European Pharmacopoeia, and the verification of the claimed dosage of PACs. Moreover, in order to confirm the real presence of cranberry in DS, chemical fingerprinting via HPLC-UV/Vis-MS/MS was employed. Our results showed that 17 DS did not comply with the uniformity test of dosage forms, and only five contained cranberry. Finally, 16 DS claimed an incorrect amount of PACs. These data suggest that several cranberry-based DS are present in the European market with insufficient quality controls. Considering that often DS are self-prescribed by consumer relying on their claim, the data obtained in this work should encourage more controls and stricter rules.

ACS Style

Giuseppe Mannino; Vita Di Stefano; Antonino Lauria; Rosa Pitonzo; Carla Gentile. Vaccinium macrocarpon (Cranberry)-Based Dietary Supplements: Variation in Mass Uniformity, Proanthocyanidin Dosage and Anthocyanin Profile Demonstrates Quality Control Standard Needed. Nutrients 2020, 12, 992 .

AMA Style

Giuseppe Mannino, Vita Di Stefano, Antonino Lauria, Rosa Pitonzo, Carla Gentile. Vaccinium macrocarpon (Cranberry)-Based Dietary Supplements: Variation in Mass Uniformity, Proanthocyanidin Dosage and Anthocyanin Profile Demonstrates Quality Control Standard Needed. Nutrients. 2020; 12 (4):992.

Chicago/Turabian Style

Giuseppe Mannino; Vita Di Stefano; Antonino Lauria; Rosa Pitonzo; Carla Gentile. 2020. "Vaccinium macrocarpon (Cranberry)-Based Dietary Supplements: Variation in Mass Uniformity, Proanthocyanidin Dosage and Anthocyanin Profile Demonstrates Quality Control Standard Needed." Nutrients 12, no. 4: 992.

Journal article
Published: 02 April 2020 in Agronomy
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Six papaya (Carica papaya L.) cultivars, grown in a Mediterranean climate under greenhouse conditions, were screened for physicochemical properties, antioxidant capacity, nutritional and sensory characteristics. The fruits, harvested with more than 50% of yellow surface (between 60% and 77%) were tested for carotenoids content, phenolic content, reducing activity (ABTS) and cellular antioxidant activity (CAA50). The physicochemical traits were measured in terms of the titratable acidity and soluble content whereas proximal composition along with moisture, fats, total sugar, ash, vitamin A, C and E content. Moreover, the sensory profile was analyzed by a semi-trained panel. Although the six analyzed cultivars reached qualitative characteristics to satisfy market needs, significant differences among them were found in a genotype-dependent manner. In particular, Cartagena and Maradol cultivars evidenced the highest values of minerals and vitamins, carotenoids, polyphenols, ABTS and CAA50 and reached the best commercial requisites (size, total soluble solids content/titratable acidity ratio). As for sensory analysis, we observed significate differences only for sweetness, juiciness, odor and flavor of peach and exotic fruits whereas descriptors related to unpleasant defects or sensations always have very low scores in all the observed cultivars. These results highlight the possibility of obtaining quality papaya fruits in a Mediterranean climate using greenhouse growing.

ACS Style

Vittorio Farina; Ilenia Tinebra; Anna Perrone; Giuseppe Sortino; Eristanna Palazzolo; Giuseppe Mannino; Carla Gentile. Physicochemical, Nutraceutical and Sensory Traits of Six Papaya (Carica papaya L.) Cultivars Grown in Greenhouse Conditions in the Mediterranean Climate. Agronomy 2020, 10, 501 .

AMA Style

Vittorio Farina, Ilenia Tinebra, Anna Perrone, Giuseppe Sortino, Eristanna Palazzolo, Giuseppe Mannino, Carla Gentile. Physicochemical, Nutraceutical and Sensory Traits of Six Papaya (Carica papaya L.) Cultivars Grown in Greenhouse Conditions in the Mediterranean Climate. Agronomy. 2020; 10 (4):501.

Chicago/Turabian Style

Vittorio Farina; Ilenia Tinebra; Anna Perrone; Giuseppe Sortino; Eristanna Palazzolo; Giuseppe Mannino; Carla Gentile. 2020. "Physicochemical, Nutraceutical and Sensory Traits of Six Papaya (Carica papaya L.) Cultivars Grown in Greenhouse Conditions in the Mediterranean Climate." Agronomy 10, no. 4: 501.

Journal article
Published: 24 January 2020 in Agronomy
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Several recent reports have highlighted some of the mechanisms involved in the enhanced tolerance to abiotic stresses induced by root-associated microorganisms, although additional efforts are still required to exploit and optimize these strategies. Particularly, arbuscular mycorrhizal fungi (AMF) play an important role as “bio-fertilizing microorganisms”, establishing mutualistic symbioses with the roots of most crops. In this work, different microbial inocula (a single AMF species, a combination of three different AMF species, a combination of two plant growth-promoting bacteria (PGPB) strains and a more complex commercial inoculum) have been used to inoculate tomato plants (cv San Marzano nano), in order to verify their effects on the tolerance to a water deficit condition in pots, through the evaluation of biochemical stress markers and hormonal profiles (ABA and IAA). Results showed differences among tomato responses to water limitation depending on microbial inocula, confirming the importance to characterize the optimal plant/microorganism genotype combination(s) to maximize plant performance and tolerance. These findings open new perspectives for a better exploitation of these microorganisms.

ACS Style

Giuseppe Mannino; Luca Nerva; Takoua Gritli; Mara Novero; Valentina Fiorilli; Mnasri Bacem; Cinzia Margherita Bertea; Erica Lumini; Walter Chitarra; Raffaella Balestrini. Effects of Different Microbial Inocula on Tomato Tolerance to Water Deficit. Agronomy 2020, 10, 170 .

AMA Style

Giuseppe Mannino, Luca Nerva, Takoua Gritli, Mara Novero, Valentina Fiorilli, Mnasri Bacem, Cinzia Margherita Bertea, Erica Lumini, Walter Chitarra, Raffaella Balestrini. Effects of Different Microbial Inocula on Tomato Tolerance to Water Deficit. Agronomy. 2020; 10 (2):170.

Chicago/Turabian Style

Giuseppe Mannino; Luca Nerva; Takoua Gritli; Mara Novero; Valentina Fiorilli; Mnasri Bacem; Cinzia Margherita Bertea; Erica Lumini; Walter Chitarra; Raffaella Balestrini. 2020. "Effects of Different Microbial Inocula on Tomato Tolerance to Water Deficit." Agronomy 10, no. 2: 170.

Journal article
Published: 12 October 2019 in Bioinformatics
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Motivation New in silico tools to predict biological affinities for input structures are presented. The tools are implemented in the DRUDIT (DRUgs DIscovery Tools) web service. The DRUDIT biological finder module is based on molecular descriptors that are calculated by the MOLDESTO (MOLecular DEScriptors TOol) software module developed by the same authors, which is able to calculate more than one thousand molecular descriptors. At this stage, DRUDIT includes 250 biological targets, but new external targets can be added. This feature extends the application scope of DRUDIT to several fields. Moreover, two more functions are implemented: the multi- and on/off-target tasks. These tools applied to input structures allow for predicting the polypharmacology and evaluating the collateral effects. Results The applications described in the article show that DRUDIT is able to predict a single biological target, to identify similarities among biological targets, and to discriminate different target isoforms. The main advantages of DRUDIT for the scientific community lie in its ease of use by worldwide scientists and the possibility to be used also without specific, and often expensive, hardware and software. In fact, it is fully accessible through the WWW from any device to perform calculations. Just a click or a tap can start tasks to predict biological properties for new compounds or repurpose drugs, lead compounds, or unsuccessful compounds. To date, DRUDIT is supported by four servers each able to execute 8 jobs simultaneously. Availability and implementation The web service is accessible at the www.drudit.com URL and its use is free of charge. Supplementary information Supplementary data are available at Bioinformatics online.

ACS Style

Antonino Lauria; Salvatore Mannino; Carla Gentile; Giuseppe Mannino; Annamaria Martorana; Daniele Peri. DRUDIT: web-based DRUgs DIscovery Tools to design small molecules as modulators of biological targets. Bioinformatics 2019, 1 .

AMA Style

Antonino Lauria, Salvatore Mannino, Carla Gentile, Giuseppe Mannino, Annamaria Martorana, Daniele Peri. DRUDIT: web-based DRUgs DIscovery Tools to design small molecules as modulators of biological targets. Bioinformatics. 2019; ():1.

Chicago/Turabian Style

Antonino Lauria; Salvatore Mannino; Carla Gentile; Giuseppe Mannino; Annamaria Martorana; Daniele Peri. 2019. "DRUDIT: web-based DRUgs DIscovery Tools to design small molecules as modulators of biological targets." Bioinformatics , no. : 1.

Journal article
Published: 01 October 2019 in Journal of Functional Foods
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Ivano Vigliante; Giuseppe Mannino; Massimo E. Maffei. OxiCyan®, a phytocomplex of bilberry (Vaccinium myrtillus) and spirulina (Spirulina platensis), exerts both direct antioxidant activity and modulation of ARE/Nrf2 pathway in HepG2 cells. Journal of Functional Foods 2019, 61, 1 .

AMA Style

Ivano Vigliante, Giuseppe Mannino, Massimo E. Maffei. OxiCyan®, a phytocomplex of bilberry (Vaccinium myrtillus) and spirulina (Spirulina platensis), exerts both direct antioxidant activity and modulation of ARE/Nrf2 pathway in HepG2 cells. Journal of Functional Foods. 2019; 61 ():1.

Chicago/Turabian Style

Ivano Vigliante; Giuseppe Mannino; Massimo E. Maffei. 2019. "OxiCyan®, a phytocomplex of bilberry (Vaccinium myrtillus) and spirulina (Spirulina platensis), exerts both direct antioxidant activity and modulation of ARE/Nrf2 pathway in HepG2 cells." Journal of Functional Foods 61, no. : 1.