To evaluate the contributions of the some quantum dots in different biological uses in order to valorizes such nanomaterials for further applications. Zinc sulfide ZnS nanoparticles were synthesized in aqueous medium at pH constant, the obtained nanoparticles has been characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier Transform Infra-red (FTIR) spectroscopies. Zinc sulfide nanoparticles were screened for their antibacterial and antifungal profiling and tested for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (OH) and hydrogen peroxide (H2O2) scavenging activity, ferric reducing power (FRP) assay and ferrous ion chelating (FIC) methods. The sizes of the crystallites were estimated to 3 nm using the Debye-Scherrer formula based on the XRD data. The shape was identified to be quasi-spherical with agglomerated particles. The obtained ZnS quantum dots present an antioxidant activity especially in oxido-reduction power, and can be used for species profiling either for bacteria and fungus. It was found that ZnS nanoparticles showed relatively higher antioxidant activities and antibacterial with an antifungal behavior which proves that this nanomaterials can react at the interface with the life entities.