This page has only limited features, please log in for full access.
Auxin is the reciprocal signaling molecule, which interferes with other phyto-hormonal and physiological processes during plant–microbes interaction. In this regard, Bipolaris spp., a growth-promoting endophytic fungus was used to inoculate pre-stressed Zea mays seedlings with yucasin (IAA inhibitor). The IAA-deficient host was heavily colonized by the endophyte that subsequently promoted the host growth and elevated the IAA levels with a peak value at 72 h. However, the seedling growth was inhibited later (i.e., at 120 h) due to the high levels of IAA that interfered with the activity of phytoalexins and brassinosteroids. Such interference also modulated the endophytic fungus from symbiotic to biotrophic pathogen that left the host plants defenseless.
Muhammad Junaid Yousaf; Anwar Hussain; Muhammad Hamayun; Amjad Iqbal; Muhammad Irshad; Ho-Youn Kim; In-Jung Lee. Transformation of Endophytic Bipolaris spp. Into Biotrophic Pathogen Under Auxin Cross-Talk With Brassinosteroids and Abscisic Acid. Frontiers in Bioengineering and Biotechnology 2021, 9, 1 .
AMA StyleMuhammad Junaid Yousaf, Anwar Hussain, Muhammad Hamayun, Amjad Iqbal, Muhammad Irshad, Ho-Youn Kim, In-Jung Lee. Transformation of Endophytic Bipolaris spp. Into Biotrophic Pathogen Under Auxin Cross-Talk With Brassinosteroids and Abscisic Acid. Frontiers in Bioengineering and Biotechnology. 2021; 9 ():1.
Chicago/Turabian StyleMuhammad Junaid Yousaf; Anwar Hussain; Muhammad Hamayun; Amjad Iqbal; Muhammad Irshad; Ho-Youn Kim; In-Jung Lee. 2021. "Transformation of Endophytic Bipolaris spp. Into Biotrophic Pathogen Under Auxin Cross-Talk With Brassinosteroids and Abscisic Acid." Frontiers in Bioengineering and Biotechnology 9, no. : 1.
Ganoderma lucidum extract is a potent traditional remedy for curing various ailments. Drying is the most important postharvest step during the processing of Ganoderma lucidum. The drying process mainly involves heat (36 h at 60 °C) and freeze-drying (36 h at −80 °C). We investigated the effects of different postharvest drying protocols on the metabolites profiling of Ganoderma lucidum using GC-MS, followed by an investigation of the anti-neuroinflammatory potential in LPS-treated BV2 microglial cells. A total of 109 primary metabolites were detected from heat and freeze-dried samples. Primary metabolite profiling showed higher levels of amino acids (17.4%) and monosaccharides (8.8%) in the heat-dried extracts, whereas high levels of organic acids (64.1%) were present in the freeze-dried samples. The enzymatic activity, such as ATP-citrate synthase, pyruvate kinase, glyceraldehyde-3-phosphatase dehydrogenase, glutamine synthase, fructose-bisphosphate aldolase, and D-3-phosphoglycerate dehydrogenase, related to the reverse tricarboxylic acid cycle were significantly high in the heat-dried samples. We also observed a decreased phosphorylation level of the MAP kinase (Erk1/2, p38, and JNK) and NF-κB subunit p65 in the heat-dried samples of the BV2 microglia cells. The current study suggests that heat drying improves the production of ganoderic acids by the upregulation of TCA-related pathways, which, in turn, gives a significant reduction in the inflammatory response of LPS-induced BV2 cells. This may be attributed to the inhibition of NF-κB and MAP kinase signaling pathways in cells treated with heat-dried extracts.
Nooruddin-Bin Sadiq; Da-Hye Ryu; Jwa-Yeong Cho; A-Hyeon Lee; Dae-Geun Song; Banzragch Dorjsembe; Jin-Chul Kim; Je-Hyeong Jung; Chu-Won Nho; Muhammad Hamayun; Seung-Hoon Yang; Ho-Youn Kim. Postharvest Drying Techniques Regulate Secondary Metabolites and Anti-Neuroinflammatory Activities of Ganoderma lucidum. Molecules 2021, 26, 4484 .
AMA StyleNooruddin-Bin Sadiq, Da-Hye Ryu, Jwa-Yeong Cho, A-Hyeon Lee, Dae-Geun Song, Banzragch Dorjsembe, Jin-Chul Kim, Je-Hyeong Jung, Chu-Won Nho, Muhammad Hamayun, Seung-Hoon Yang, Ho-Youn Kim. Postharvest Drying Techniques Regulate Secondary Metabolites and Anti-Neuroinflammatory Activities of Ganoderma lucidum. Molecules. 2021; 26 (15):4484.
Chicago/Turabian StyleNooruddin-Bin Sadiq; Da-Hye Ryu; Jwa-Yeong Cho; A-Hyeon Lee; Dae-Geun Song; Banzragch Dorjsembe; Jin-Chul Kim; Je-Hyeong Jung; Chu-Won Nho; Muhammad Hamayun; Seung-Hoon Yang; Ho-Youn Kim. 2021. "Postharvest Drying Techniques Regulate Secondary Metabolites and Anti-Neuroinflammatory Activities of Ganoderma lucidum." Molecules 26, no. 15: 4484.
Plants are susceptible to various environmental constrains, including heat stress due to their sessile nature. Endophytic fungi can be used as a novel technique to protect crop plants against the injurious effects of thermal stress. Endophytic fungi were isolated from Adiantum capillus-veneris L. and tested against heat stress in Glycine max L. and Helianthus annuus L. The results exhibited increased levels of the plant’s chlorophyll, height and biomass in Aspergillus foetidus (AdR-13) inoculated host crop species. Conversely, a significant decrease in lipid peroxidation and reactive oxygen species (ROS) production was noted in A. foetidus-associated host crop species. Likewise, the amounts of ROS-degrading antioxidants (glutathione reductase (GR), peroxidase (POD), ascorbic acid oxidase (AAO), superoxide dismutase (SOD), catalase (CAT)) as well as phenolics were increased, while the amounts of proline and abscisic acid (ABA) were decreased in fungal-associated test crops. Total lipids, proteins and sugars were noted to be high in A. foetidus-associated test crops. From the results, we concluded that A. foetidus have a role in heat stress mitigation that might help to sustain the production of important crops in the future.
Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Khan; Ayaz Ahmad; Sarah Gul; Ho-Youn Kim; In-Jung Lee. Aspergillus foetidus Regulated the Biochemical Characteristics of Soybean and Sunflower under Heat Stress Condition: Role in Sustainability. Sustainability 2021, 13, 7159 .
AMA StyleIsmail, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Sumera Khan, Ayaz Ahmad, Sarah Gul, Ho-Youn Kim, In-Jung Lee. Aspergillus foetidus Regulated the Biochemical Characteristics of Soybean and Sunflower under Heat Stress Condition: Role in Sustainability. Sustainability. 2021; 13 (13):7159.
Chicago/Turabian StyleIsmail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Khan; Ayaz Ahmad; Sarah Gul; Ho-Youn Kim; In-Jung Lee. 2021. "Aspergillus foetidus Regulated the Biochemical Characteristics of Soybean and Sunflower under Heat Stress Condition: Role in Sustainability." Sustainability 13, no. 13: 7159.
High temperature is one of the compelling ecological stresses faced by the plants owing to anthropogenic activities of mankind. Recently, endophytic fungi are considered to be a novel tool to counteract the consequences of thermal stress in crops. Owing to the problem, we isolated fungal endophytes from Dryopteris blanfordii and their growth favoring potential in rice seedlings. Endophytic fungi have the proficiency to secrete plant growth stimulating secondary metabolites that help the host plants to restore growth under harsh environments. The isolated Penicillium glabrum (DryR- 30) helped the host plant species, i.e. Glycine max L. and Helianthus annuus L. under thermal stress. P. glabrum associated with G. max and H. annuus exposed to 40ºC not only boosted their host growth attributes (total biomass, plant height and chlorophyll contents), but also significantly curtailed the synthesis of reactive oxygen species and lipids peroxidation. Moreover, the abscisic acid (ABA) and proline concentrations were significantly reduced, while the accumulation of ROS-degrading enzymes, such as catalase (CAT), peroxidase (POD), ascorbic acid oxidase (AAO), glutathione reductase (GR) and superoxide dismutase (SOD) were increased in P. glabrum-associated crops. Additionally, the protein, lipid and sugar contents in tested plants were also improved. These stirring findings suggest that P. glabrum can be applied to food crops as a thermal stress alleviation tool.
Ismail Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Sarah Gul; Hamayoon Khan; Khushnood Ur Rehman; Hamida Bibi; In-Jung Lee. Penicillium Glabrum Acted as a Heat Stress Relieving Endophyte in Soybean and Sunflower. Polish Journal of Environmental Studies 2021, 30, 3099 -3110.
AMA StyleIsmail Ismail, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Sumera Afzal Khan, Sarah Gul, Hamayoon Khan, Khushnood Ur Rehman, Hamida Bibi, In-Jung Lee. Penicillium Glabrum Acted as a Heat Stress Relieving Endophyte in Soybean and Sunflower. Polish Journal of Environmental Studies. 2021; 30 (4):3099-3110.
Chicago/Turabian StyleIsmail Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Sarah Gul; Hamayoon Khan; Khushnood Ur Rehman; Hamida Bibi; In-Jung Lee. 2021. "Penicillium Glabrum Acted as a Heat Stress Relieving Endophyte in Soybean and Sunflower." Polish Journal of Environmental Studies 30, no. 4: 3099-3110.
Heavy metal contamination due to anthropogenic activities is a great threat to modern humanity. A novel and natural technique of bioremediation using microbes for detoxification of HMs while improving plants' growth is the call of the day. In this study, exposing soybean plants to different concentrations (i.e., 10 and 50 µg/mL) of chromium and arsenic showed a severe reduction in agronomic attributes, higher ROS production, and disruption in the antioxidant system. Contrarily, rhizobacterial isolate C18 inoculation not only rescued host growth, but also improved the production of nonenzymatic antioxidants (i.e., flavonoids, phenolic and proline contents) and enzymatic antioxidants (i.e., CAT, APX, POD, and DPPH), higher ROS scavenging, and lower ROS accumulation. Thereby, lowering secondary oxidative stress and subsequent damage. The strain was identified using 16S rDNA sequencing, and was identified as Pseudocitrobacter anthropi. Additionally, the strain can endure metals up to 1200 µg/mL and efficient in detoxifying the effect of Cr and As, by regulating phytohormones (IAA 59.02 μg/mL and GA 101.88 nM/mL) and solubilizing inorganic phosphates, making them excellent phytostimulant, biofertilizers, and heavy metal bio-remediating agent.
Husna Husna; Anwar Hussain; Mohib Shah; Muhammad Hamayun; Amjad Iqbal; Waheed Murad; Muhammad Irshad; Muhammad Qadir; Ho-Youn Kim. Pseudocitrobacter Anthropi Reduces Heavy Metal Uptake and Improves Phytohormones and Antioxidant System in Glycine Max L. 2021, 1 .
AMA StyleHusna Husna, Anwar Hussain, Mohib Shah, Muhammad Hamayun, Amjad Iqbal, Waheed Murad, Muhammad Irshad, Muhammad Qadir, Ho-Youn Kim. Pseudocitrobacter Anthropi Reduces Heavy Metal Uptake and Improves Phytohormones and Antioxidant System in Glycine Max L. . 2021; ():1.
Chicago/Turabian StyleHusna Husna; Anwar Hussain; Mohib Shah; Muhammad Hamayun; Amjad Iqbal; Waheed Murad; Muhammad Irshad; Muhammad Qadir; Ho-Youn Kim. 2021. "Pseudocitrobacter Anthropi Reduces Heavy Metal Uptake and Improves Phytohormones and Antioxidant System in Glycine Max L." , no. : 1.
Drought stress is a prevalent environmental stress that adversely affects agricultural industries worldwide. In this study, bacterial isolates, AFFR02 and Mj1212, showed tolerance to polyethylene glycol-induced (PEG) drought stress (approximately 15%) and possess strong phosphate-solubilizing capacity. Moreover, we investigated the plant growth attributes, chlorophyll content, and ion uptake in alfalfa plants (Medicago sativa L) inoculated with isolates AFFR02 and Mj1212 under drought stress. We observed that drought stress drastically affects alfalfa’s growth attributes: shoot length: SL (24.88%), root length: RL (29.62%), shoot fresh weight: SFW (49.62%), root fresh weight: RFW (45.09%), stalk diameter: SD (52.84%), and chlorophyll content: CC (19.2%). However, in bacterial-inoculated alfalfa plants, the growth attributes significantly recovered were SL (12.42%), RL (21.30%), SFW (50.74%), RFW (46.42%), SD (76.72%), and CC (17.98%). In drought-stressed alfalfa plants, we observed a significant decrease in the relative water content (7.45%), whereas there was an increase in electrical conductivity (68.87%) and abscisic acid contents (164.42%). Antioxidant analysis showed a significant increase in total phenolic content (46.08%), DPPH-scavenging activity (39.66%), total flavonoid (13.68%), and superoxide dismutase (28.51%) in alfalfa treated with drought stress and bacterial isolates AFFR02 and Mj1212 simultaneously. Moreover, an increase in inductively coupled plasma (ICP) analysis of potassium (17.98%), phosphorous (11.14%), calcium (3.07%), and magnesium (6.71%) was recorded for bacteria-inoculated alfalfa plants under drought stress. In conclusion, bacterial isolates AFFR02 and Mj1212 enhance alfalfa growth under drought stress. Therefore, the isolates could be used as potential candidates in smart-climate agricultural practices in drought-stricken areas worldwide.
Sang-Mo Kang; Muhammad-Aaqil Khan; Muhammad Hamayun; Lee-Rang Kim; Eun-Hae Kwon; Yo-Sep Kang; Ki-Yong Kim; Jae-Jeong Park; In-Jung Lee. Phosphate-Solubilizing Enterobacter ludwigii AFFR02 and Bacillus megaterium Mj1212 Rescues Alfalfa’s Growth under Post-Drought Stress. Agriculture 2021, 11, 485 .
AMA StyleSang-Mo Kang, Muhammad-Aaqil Khan, Muhammad Hamayun, Lee-Rang Kim, Eun-Hae Kwon, Yo-Sep Kang, Ki-Yong Kim, Jae-Jeong Park, In-Jung Lee. Phosphate-Solubilizing Enterobacter ludwigii AFFR02 and Bacillus megaterium Mj1212 Rescues Alfalfa’s Growth under Post-Drought Stress. Agriculture. 2021; 11 (6):485.
Chicago/Turabian StyleSang-Mo Kang; Muhammad-Aaqil Khan; Muhammad Hamayun; Lee-Rang Kim; Eun-Hae Kwon; Yo-Sep Kang; Ki-Yong Kim; Jae-Jeong Park; In-Jung Lee. 2021. "Phosphate-Solubilizing Enterobacter ludwigii AFFR02 and Bacillus megaterium Mj1212 Rescues Alfalfa’s Growth under Post-Drought Stress." Agriculture 11, no. 6: 485.
Salinity is a significant abiotic stress for crop plants and a threat to global food security. Optimizing yield without adversely affecting the ecosystem is necessary for a sustainable agriculture. Silicon and plant growth-promoting bacteria were reported for mitigating several abiotic and biotic stress in plants. In our study, we identified the salt-tolerant rhizobacterium Pseudomonas psychrotolerans CS51. This species produces several plant-growth-promoting biochemicals like indole-3-acetic acid (33 ± 1.8 ng/mL) and gibberellic acid (GA3; 38 ± 1.3 and GA4; 23 ± 1.2 ng/mL) in Luria-Bertani(LB) media, and LB media spiked with 200 mM NaCl (indole-3-acetic acid(IAA); 17.6 ± 0.4 ng/mL, GA3; 21 ± 0.9 and GA4; 19 ± 1.0 ng/mL). In the current study, we aimed to investigate the effect of isolate CS51 and exogenous silicon (3 mM) on maize under salinity stress (200 mM). Our results showed that the sole application of isolate CS51, Si, and combined CS51 + Si significantly enhanced maize biomass and chlorophyll content under normal and salinity stress. Phytohormonal results showed that salinity stress increased abscisic acid (ABA; three folds) and jasmonic acid (JA; 49.20%). However, the sole and combined isolate CS51 + Si application markedly reduced ABA (1.5 folds) and JA content (14.89%). Besides, the sole and isolate CS51 + Si co-application strengthened the antioxidant system, such as flavonoid (97%) and polyphenol (19.64%), and lowered the proline content (57.69%) under NaCl stress. Similarly, the CS51 and Si inoculation (solely or combined) significantly enhanced the Si uptake (4 folds) and reduced the Na+ uptake (42.30%) in maize plants under NaCl stress. In conclusion, the current finding suggests that combining CS51 with Si can be used against salinity stress in maize plants and may be commercialized as a biofertilizer.
Happy Kubi; Muhammad Khan; Arjun Adhikari; Muhammad Imran; Sang-Mo Kang; Muhammad Hamayun; In-Jung Lee. Silicon and Plant Growth-Promoting Rhizobacteria Pseudomonas psychrotolerans CS51 Mitigates Salt Stress in Zea mays L. Agriculture 2021, 11, 272 .
AMA StyleHappy Kubi, Muhammad Khan, Arjun Adhikari, Muhammad Imran, Sang-Mo Kang, Muhammad Hamayun, In-Jung Lee. Silicon and Plant Growth-Promoting Rhizobacteria Pseudomonas psychrotolerans CS51 Mitigates Salt Stress in Zea mays L. Agriculture. 2021; 11 (3):272.
Chicago/Turabian StyleHappy Kubi; Muhammad Khan; Arjun Adhikari; Muhammad Imran; Sang-Mo Kang; Muhammad Hamayun; In-Jung Lee. 2021. "Silicon and Plant Growth-Promoting Rhizobacteria Pseudomonas psychrotolerans CS51 Mitigates Salt Stress in Zea mays L." Agriculture 11, no. 3: 272.
This study aimed to evaluate the effect of endophytic fungi isolated from Cucurbita roots on growth, physiological, biochemical and molecular attributes of Solanum lycopersicum L. under Cd and Cr stress. The initial screening experiments on Oryza sativa L. seedlings revealed a plant growth promoting endophyte (Cu-17) out of the 17 isolated strains. The strain was identified as Aspergillus niger by amplifying ITS region of 18 S rDNA. A pot experiment was set to evaluate the effect of A. niger on S. lycopersicum exposed to two different concentrations (100 μg/g and 500 μg/g) of Cd and Cr. A. niger alleviated Cd and Cr stress in S. lycopersicum by reducing its translocation to the aerial parts of the host plant. A. niger also enabled the S. lycopersicum to produce significant amounts of IAA, proline, flavonoids, phenols, catalase (CAT) and ascorbic acid oxidase (AAO), in addition to sugar and proteins. Moreover, A. niger facilitated the S. lycopersicum to induce stress responsive genes (SlGSH1 and SlPCS1) in order to develop tolerance against the Cd and Cr stress. Both SlGSH1 and SlPCS1 genes helped the S. lycopersicum to chelate Cd and Cr and thus mitigate their toxicity. The results, therefore, conclude that A. niger might be used as biofertilizer for healthy and safe crop production in Cd and Cr contaminated soils.
Laila Aziz; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Muhammad Arif; Anwar Husssin; Sumera Afzal Khan. Endophytic Aspergillus niger reprograms the physicochemical traits of tomato under cadmium and chromium stress. Environmental and Experimental Botany 2021, 186, 104456 .
AMA StyleLaila Aziz, Muhammad Hamayun, Mamoona Rauf, Amjad Iqbal, Muhammad Arif, Anwar Husssin, Sumera Afzal Khan. Endophytic Aspergillus niger reprograms the physicochemical traits of tomato under cadmium and chromium stress. Environmental and Experimental Botany. 2021; 186 ():104456.
Chicago/Turabian StyleLaila Aziz; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Muhammad Arif; Anwar Husssin; Sumera Afzal Khan. 2021. "Endophytic Aspergillus niger reprograms the physicochemical traits of tomato under cadmium and chromium stress." Environmental and Experimental Botany 186, no. : 104456.
Cadmium stress significantly decreases agricultural productivity worldwide. Plant growth-promoting rhizobacteria (PGPR) are eco-friendly and inexpensive tool for mitigating heavy metal stress in crops. We isolated rhizospheric bacteria and screened them for various plant growth-promoting (PGP) traits as well as Cd tolerance. Only 6 bacterial isolates out of 55 assessed showed multiple PGP traits in response to different Cd concentrations. The Bacillus cereus ALT1 strain showed high tolerance to increased Cd amounts in the culture medium, while secreting indole-3-acetic acid (IAA) and organic acids into the culture medium. High Cd concentrations (0.7 mM, 1.4 mM, and 2.1 mM) reduced soybean shoot and root length, root/shoot fresh and dry weight, as well as chlorophyll content; however, inoculation with the bacterial isolate ALT1 mitigated Cd stress and enhanced both soybean growth parameters and chlorophyll content. It also decreased abscisic acid (ABA) amounts, enhanced salicylic acid (SA) production, and promoted antioxidant response by increasing total proteins (TP) and superoxide dismutase (SOD), while decreasing glutathione (GSH) content, lipid peroxidation (LPO), peroxidase (POD), superoxide anion (SOA), and polyphenol oxidase (PPO) in soybean plants. In addition, inductively coupled plasma mass spectrometry (ICP-MS) showed that soybean plants treated with the bacterial isolate ALT1 enhanced K uptake and decreased Cd amounts in comparison to control plants. The present study reveals that Cd-tolerant bacterial isolate ALT1 can alleviate Cd toxicity on plants by increasing their growth, thus imposing itself as an eco-friendly bio-fertilizer under Cd stress.
Atlaw Sahile; Muhammad Khan; Muhammad Hamayun; Muhammad Imran; Sang-Mo Kang; In-Jung Lee. Novel Bacillus cereus Strain, ALT1, Enhance Growth and Strengthens the Antioxidant System of Soybean under Cadmium Stress. Agronomy 2021, 11, 404 .
AMA StyleAtlaw Sahile, Muhammad Khan, Muhammad Hamayun, Muhammad Imran, Sang-Mo Kang, In-Jung Lee. Novel Bacillus cereus Strain, ALT1, Enhance Growth and Strengthens the Antioxidant System of Soybean under Cadmium Stress. Agronomy. 2021; 11 (2):404.
Chicago/Turabian StyleAtlaw Sahile; Muhammad Khan; Muhammad Hamayun; Muhammad Imran; Sang-Mo Kang; In-Jung Lee. 2021. "Novel Bacillus cereus Strain, ALT1, Enhance Growth and Strengthens the Antioxidant System of Soybean under Cadmium Stress." Agronomy 11, no. 2: 404.
In the present study, we have isolated endophytic fungi Gliocladium cibotii from Verbena officinalis L. and tested against Oryza sativa L. seedlings for growth promotion. The strain was used to help Glycine max L. and Helianthus annuus L. under thermal stress. Association of G. cibotii with G. max and H. annuus enhanced chlorophyll contents, total biomass and plant height. Also, G. cibotii significantly curtailed lipids peroxidation and ROS (reactive oxygen species) in host species exposed to heat stress (40ºC). Furthermore, the concentration of ROS-degrading enzymes, like ascorbic acid oxidase (AAO), catalase (CAT), glutathione reductase (GR), peroxidase (POD) and superoxide dismutase (SOD) were boosted in test crops, while concentration of proline and ABA were inhibited. The phenolics and nutritional value (total lipids, proteins and sugars) of experimental plants were also enhanced. The results conclude that G. cibotii can be used as a heat stress mitigating weapon for food crops in the future.
Ismail Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Muhammad Aaqil Khan; In-Jung Lee. An Endophytic Fungus Gliocladium cibotii Regulates Metabolic and Antioxidant System of Glycine max and Helianthus annuus under Heat Stress. Polish Journal of Environmental Studies 2021, 30, 1631 -1640.
AMA StyleIsmail Ismail, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Sumera Afzal Khan, Muhammad Aaqil Khan, In-Jung Lee. An Endophytic Fungus Gliocladium cibotii Regulates Metabolic and Antioxidant System of Glycine max and Helianthus annuus under Heat Stress. Polish Journal of Environmental Studies. 2021; 30 (2):1631-1640.
Chicago/Turabian StyleIsmail Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Muhammad Aaqil Khan; In-Jung Lee. 2021. "An Endophytic Fungus Gliocladium cibotii Regulates Metabolic and Antioxidant System of Glycine max and Helianthus annuus under Heat Stress." Polish Journal of Environmental Studies 30, no. 2: 1631-1640.
Muhammad Hamayun; Nasir Khan; Muhammad Nauman Khan; Muhammad Qadir; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Khushnood Ur Rehman; In-Jung Lee. Antimicrobial and plant growth-promoting activities of bacterial endophytes isolated from Calotropis procera (Ait.) W.T. Aiton. BIOCELL 2021, 45, 363 -369.
AMA StyleMuhammad Hamayun, Nasir Khan, Muhammad Nauman Khan, Muhammad Qadir, Anwar Hussain, Amjad Iqbal, Sumera Afzal Khan, Khushnood Ur Rehman, In-Jung Lee. Antimicrobial and plant growth-promoting activities of bacterial endophytes isolated from Calotropis procera (Ait.) W.T. Aiton. BIOCELL. 2021; 45 (2):363-369.
Chicago/Turabian StyleMuhammad Hamayun; Nasir Khan; Muhammad Nauman Khan; Muhammad Qadir; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Khushnood Ur Rehman; In-Jung Lee. 2021. "Antimicrobial and plant growth-promoting activities of bacterial endophytes isolated from Calotropis procera (Ait.) W.T. Aiton." BIOCELL 45, no. 2: 363-369.
Cadmium (Cd) and chromium (Cr) in agricultural soils is becoming a serious threat in tomato. The complete removal of these heavy metals (HMs) from contaminated soil is challenging. However, the use of plant endophytes might be beneficial. The pragmatic role of endophytic fungi Aspergillus flavus (Ch-01) in tomato under Cd and Cr stress was, therefore, investigated. A. flavus enhanced growth attributes in tomato seedlings under Cd and Cr stress. Besides, it remediated Cd and Cr toxicity by reducing their translocation from the roots to the upper part of the tomato plants. In fact, A. flavus associated plants developed tolerance against Cd and Cr toxicity via the expression of SlGSH1 and SlPCS1 genes. Both genes helped in metal chelation and mitigation of Cd and Cr toxicity. From the results, it is concluded that A. flavus is a plant growth promoting and HMs alleviating endophyte, hence it might be used as a biofertilizer.
Laila Aziz; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Anwar Husssin; Sumera Afzal Khan; Muhammad Irshad; In-Jung Lee. Aspergillus Flavus reprogrammed morphological and chemical attributes of Solanum lycopersicum through SlGSH1 and SlPCS1 genes modulation under heavy metal stress. Journal of Plant Interactions 2021, 16, 104 -115.
AMA StyleLaila Aziz, Muhammad Hamayun, Mamoona Rauf, Amjad Iqbal, Anwar Husssin, Sumera Afzal Khan, Muhammad Irshad, In-Jung Lee. Aspergillus Flavus reprogrammed morphological and chemical attributes of Solanum lycopersicum through SlGSH1 and SlPCS1 genes modulation under heavy metal stress. Journal of Plant Interactions. 2021; 16 (1):104-115.
Chicago/Turabian StyleLaila Aziz; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Anwar Husssin; Sumera Afzal Khan; Muhammad Irshad; In-Jung Lee. 2021. "Aspergillus Flavus reprogrammed morphological and chemical attributes of Solanum lycopersicum through SlGSH1 and SlPCS1 genes modulation under heavy metal stress." Journal of Plant Interactions 16, no. 1: 104-115.
Today's world needs to control the industrial pollution through smarter ways. Presently, we observed the capacity of soil borne fungi to digest Synozol Red HF–6BN and Synozol Black B. Initially, 86 fungal strains were isolated from soil samples randomly collected from industrial sites. Among these, 31 isolates were capable of dye decolorization on solid media, with SN12f and SN13a isolates showed the highest decolorization capacity. The dye decolorization by both strains was higher (80–95%), when incubated for 120 h under optimized conditions of pH, concentration, nutrient source and temperature. The dye (Synozol red HF–6BN and Synozol black B) decolorization by SN12f isolate was maximum (˃90%) at pH7, whereas the SN13a decolorized 90% of Synozol red HF–6BN and 89% of Synozol black B at pH3. The SN13a and SN12f isolates at 40 mg/L showed de-colorization of 94.71%, 81.4% (for Synozol red HF–6BN) and 90.5%, 84.4% (Synozol black B), respectively. Our isolates also mitigated the toxic effect of azo dyes on the growth of phosphate solubilizing soil bacteria. In fact, the untreated effluent showed toxic effects on the growth of beneficial bacterial by developing zone of inhibition (16.5 mm around Aeromonas spp., 14.5 mm around Sallmonella while 14.25 mm around Citrobacter spp). However, the fungal treated dye was unable to develop zone of inhibition. Laccase activity was positive for both of fungal isolates after incubation on Bassnell Hass Medium (0.0733 U/mL for SN12f and 0.0439 U/mL SN13a). Using molecular approaches (ITS region), SN12f was identified as Aspergillus nidulans, while SN13a as Aspergillus fumigatus. The current study showed that local fungal flora can reclaim the contaminated soils and support the agro-friendly micro-flora.
Sumera Afzal Khan; Saadat Mehmood; Nabeela; Amjad Iqbal; Muhammad Hamayun. Industrial polluted soil borne fungi decolorize the recalcitrant azo dyes Synozol red HF–6BN and Synozol black B. Ecotoxicology and Environmental Safety 2020, 206, 111381 .
AMA StyleSumera Afzal Khan, Saadat Mehmood, Nabeela, Amjad Iqbal, Muhammad Hamayun. Industrial polluted soil borne fungi decolorize the recalcitrant azo dyes Synozol red HF–6BN and Synozol black B. Ecotoxicology and Environmental Safety. 2020; 206 ():111381.
Chicago/Turabian StyleSumera Afzal Khan; Saadat Mehmood; Nabeela; Amjad Iqbal; Muhammad Hamayun. 2020. "Industrial polluted soil borne fungi decolorize the recalcitrant azo dyes Synozol red HF–6BN and Synozol black B." Ecotoxicology and Environmental Safety 206, no. : 111381.
Ginseng is a perennial herb used as valuable traditional medicine for more than three thousand years. Ginsenosides are the major group of pharmacologically active components of ginseng, and they are effective against various diseases. Present study was designed to investigate the impact of foliar silicon sprays on growth characteristics, physiological traits, and pharmaceutical quality of 6-year-old ginseng plants. Three different concentrations i.e. 50 mg/L, 100 mg/L, and 200 mg/L of Si was used as foliar treatment. Compared to control, stem hardness was enhanced up to 54% in 100 mg/L treatment followed by 38% in 50 mg/L and 36% in 200 mg/L. SEM analysis showed that the density of epidermal cells was 21-25% higher in Si-treated plants than that in the control plants. Cuticle thickness was found higher in Si treated plants as compared to control. Overall a total of 25%, 38%, and 54% of cuticle thickness and 43%, 49%, and 63% ginseng roots production was observed in plants treated with Si treatment of 50 mg/L, 100 mg/L, and 200 mg/L, respectively. Results suggest that out of three different concentrations of Si treatments, 200 mg/L found to be most effective in overall higher Si content in leaves with thick cuticle and higher root production, 100 mg/L treatment showed high photosynthesis rate followed by stomatal conductance, chlorophyll content and stem hardness. 50 mg/L was found to be least effective. Out of 12 ginsenosides analyzed in 6-year-old ginseng roots, the most common were AP, Rg1, Rb1, and Re. The content of ginsenosides per gram of ginseng roots did not show any significant differences among treatments, but overall, ginsenosides contents were higher in Si-treated plants because of increased root growth triggered by increased Si level. Our study show that the application of aerial sprays with elevated Si levels increases the growth, promotes the physiological and morphological traits and increases the yield of 6-year-old ginseng plants. In addition, this study implied the importance of Si for commercial production of high-quality ginseng roots.
Soo-Won Jang; Nooruddin Bin Sadiq; Muhammad Hamayun; Jehyeong Jung; Taeksung Lee; Jung-Seok Yang; Bokyung Lee; Ho-Youn Kim. Silicon foliage spraying improves growth characteristics, morphological traits, and root quality of Panax ginseng C.A.Mey. Industrial Crops and Products 2020, 156, 112848 .
AMA StyleSoo-Won Jang, Nooruddin Bin Sadiq, Muhammad Hamayun, Jehyeong Jung, Taeksung Lee, Jung-Seok Yang, Bokyung Lee, Ho-Youn Kim. Silicon foliage spraying improves growth characteristics, morphological traits, and root quality of Panax ginseng C.A.Mey. Industrial Crops and Products. 2020; 156 ():112848.
Chicago/Turabian StyleSoo-Won Jang; Nooruddin Bin Sadiq; Muhammad Hamayun; Jehyeong Jung; Taeksung Lee; Jung-Seok Yang; Bokyung Lee; Ho-Youn Kim. 2020. "Silicon foliage spraying improves growth characteristics, morphological traits, and root quality of Panax ginseng C.A.Mey." Industrial Crops and Products 156, no. : 112848.
The dried Ganoderma lucidum (GL) has been widely used for its pharmacological properties and bioactive ganoderic acids (GAs). Herein, extraction procedures combining ultra-sonication and heating were optimized using response surface methodology based on four variables (antioxidant activity, anti-diabetic activity, total GAs content, and total polysaccharide content) and principal component analysis. The extraction of freeze-dried GL at temperatures between 64.2 and 70 °C for 1.2 h maximized the antioxidant activity and GA content, whereas the polysaccharide content and anti-diabetic activity were maximized by extraction between 66.8 and 70 °C for more than 2.8 h. Heat-dried GL extracted at 50 °C for 3 h provided the greatest anti-inflammatory activity against HaCaT cells by suppressing the response to inflammation related cytokines at mRNA levels. These results suggest that extraction conditions might be a limiting factor for target-oriented investigations, and optimized extraction methods may improve the potential effect and quality of harvested GL products.
Da Hye Ryu; Jwa Yeong Cho; Nooruddin Bin Sadiq; Jin-Chul Kim; Bokyung Lee; Muhammad Hamayun; Taek Sung Lee; Hyoung Seok Kim; Soo Hyun Park; Chu Won Nho; Ho-Youn Kim. Optimization of antioxidant, anti-diabetic, and anti-inflammatory activities and ganoderic acid content of differentially dried Ganoderma lucidum using response surface methodology. Food Chemistry 2020, 335, 127645 .
AMA StyleDa Hye Ryu, Jwa Yeong Cho, Nooruddin Bin Sadiq, Jin-Chul Kim, Bokyung Lee, Muhammad Hamayun, Taek Sung Lee, Hyoung Seok Kim, Soo Hyun Park, Chu Won Nho, Ho-Youn Kim. Optimization of antioxidant, anti-diabetic, and anti-inflammatory activities and ganoderic acid content of differentially dried Ganoderma lucidum using response surface methodology. Food Chemistry. 2020; 335 ():127645.
Chicago/Turabian StyleDa Hye Ryu; Jwa Yeong Cho; Nooruddin Bin Sadiq; Jin-Chul Kim; Bokyung Lee; Muhammad Hamayun; Taek Sung Lee; Hyoung Seok Kim; Soo Hyun Park; Chu Won Nho; Ho-Youn Kim. 2020. "Optimization of antioxidant, anti-diabetic, and anti-inflammatory activities and ganoderic acid content of differentially dried Ganoderma lucidum using response surface methodology." Food Chemistry 335, no. : 127645.
Chemical crosstalk between plant roots and associated microbes is imperative for the initiation of symbiosis. Phenolics, flavonoids and indole-3-acetic acid (IAA) are acclaimed as signaling molecules in developing plant-microbe symbiosis. The role of IAA and flavonoids in establishing a symbiotic relationship between Aspergillus nomius wlg2 and maize roots have also been observed during the present study. The isolate A. nomius wlg2 and maize roots have secreted flavonoids and IAA that helped in A. nomius-maize association. However, the suppression of either flavonoids or IAA secretion reduced the endophyte colonization of the maize roots by 77% and 67%, respectively. The main flavonoids secreted by maize were identified as luteolin, calycosin, phenolics cis-caftaric acid, and caffeoyl-d-glucose. The identified flavonoids were most likely to be involved in the root-endophyte association. To establish a plant-microbe interaction, the complex chemical dialogue is necessary for which IAA and flavonoids play a central role.
Asif Mehmood; Anwar Hussain; Muhammad Irshad; Muhammad Hamayun; Amjad Iqbal; Abdul Tawab; Naeem Khan. Yucasin and cinnamic acid inhibit IAA and flavonoids biosynthesis minimizing interaction between maize and endophyte Aspergillus nomius. Symbiosis 2020, 81, 149 -160.
AMA StyleAsif Mehmood, Anwar Hussain, Muhammad Irshad, Muhammad Hamayun, Amjad Iqbal, Abdul Tawab, Naeem Khan. Yucasin and cinnamic acid inhibit IAA and flavonoids biosynthesis minimizing interaction between maize and endophyte Aspergillus nomius. Symbiosis. 2020; 81 (2):149-160.
Chicago/Turabian StyleAsif Mehmood; Anwar Hussain; Muhammad Irshad; Muhammad Hamayun; Amjad Iqbal; Abdul Tawab; Naeem Khan. 2020. "Yucasin and cinnamic acid inhibit IAA and flavonoids biosynthesis minimizing interaction between maize and endophyte Aspergillus nomius." Symbiosis 81, no. 2: 149-160.
Contamination of agricultural land with heavy metal is a serious biological and environmental issue. Such threat can be challenged by exploring the plant symbiotic microbes that can improve plant growth through phyto-hormones secretion and chromate chelation. In the current study, chromate resistant rhizospheric Staphylococcus arlettae strain MT4 was isolated from the rhizosphere of Malvestrum tricuspadatum L. The strain showed potential to secrete phytohormones and plant growth promoting secondary metabolites under induced chromate stress, making it a best suitable candidate in chromate stress alleviation. Moreover, the rhizobacterium MT4 significantly promoted the net assimilation and relative growth rate of sunflower grown in the presence of chromate (100 ppm). Chromate stress alleviation strategy of MT4 strain was three-fold. MT4 alleviated chromate stress and promoted the sunflower growth by suppressing the chromate intake by the host, modulating phytohormones and strengthening of the host’s antioxidant system. The improved antioxidant system was confirmed by noticing lower ROS accumulation and improved ROS scavenging, lower peroxidase activity and higher accumulation of phenols and flavonoids.
Muhammad Qadir; Anwar Hussain; Muhammad Hamayun; Mohib Shah; Amjad Iqbal; Husna; Waheed Murad. Phytohormones producing rhizobacterium alleviates chromium toxicity in Helianthus annuus L. by reducing chromate uptake and strengthening antioxidant system. Chemosphere 2020, 258, 127386 .
AMA StyleMuhammad Qadir, Anwar Hussain, Muhammad Hamayun, Mohib Shah, Amjad Iqbal, Husna, Waheed Murad. Phytohormones producing rhizobacterium alleviates chromium toxicity in Helianthus annuus L. by reducing chromate uptake and strengthening antioxidant system. Chemosphere. 2020; 258 ():127386.
Chicago/Turabian StyleMuhammad Qadir; Anwar Hussain; Muhammad Hamayun; Mohib Shah; Amjad Iqbal; Husna; Waheed Murad. 2020. "Phytohormones producing rhizobacterium alleviates chromium toxicity in Helianthus annuus L. by reducing chromate uptake and strengthening antioxidant system." Chemosphere 258, no. : 127386.
Background: Brucellosis is an economically important zoonotic disease caused by the gram negative bacteria belonging to the genus Brucella. Medicinal plants are well known for a wide variety of potential antimicrobial agents that can be used as anti-microbial drugs. Method: In the present study, crude ethanol and methanol extracts of local plants (Berberies lyceum and Fagonia cretica) were tested in vitro against Brucella melitensis via well diffusion method for their antibacterial activity. In in-silico study, phytochemicals previously identified in the selected plants were docked with homology model of the cytotoxic factor malate synthase G (MSG) highly conserved among Brucella spp., in Molecular Operating Environment (MOE) to predict a potential drug against B. melitensis. Molecular dynamic simulation was performed to predict the stability of MSG through MOE. Result: Ethanolic crude extracts of B. lyceum showed maximum zone of inhibition (32.5 mm) followed by methanolic extracts (30 mm), while ethanolic extracts of F. cretica showed zone of inhibition (29 mm) followed by methanolic extracts (27.5 mm). In silico screening predicted phytic acid as the most potent inhibitor followed by jehlumine, barbamine, oxyberberine and sindamine. Conclusion: The synergistic utilization of phytochemicals derived from B. lyceum may potentially provide protection against B. melitensis.
Ijaz Muhammad; Sadaf Niaz; Anwar Hussain; Saghir Ahmad; Noor Rahman; Haroon Khan; Abid Ali; Gul E Nayab. Molecular docking and in-vitro analysis of Fagonia cretica and Berberis lyceum extract against Brucella melitensis. Current Computer Aided-Drug Design 2020, 16, 1 -14.
AMA StyleIjaz Muhammad, Sadaf Niaz, Anwar Hussain, Saghir Ahmad, Noor Rahman, Haroon Khan, Abid Ali, Gul E Nayab. Molecular docking and in-vitro analysis of Fagonia cretica and Berberis lyceum extract against Brucella melitensis. Current Computer Aided-Drug Design. 2020; 16 ():1-14.
Chicago/Turabian StyleIjaz Muhammad; Sadaf Niaz; Anwar Hussain; Saghir Ahmad; Noor Rahman; Haroon Khan; Abid Ali; Gul E Nayab. 2020. "Molecular docking and in-vitro analysis of Fagonia cretica and Berberis lyceum extract against Brucella melitensis." Current Computer Aided-Drug Design 16, no. : 1-14.
Plants can be severely affected by heat stress due to rapid environmental changes. Use of endophytic fungi is a new tool to protect crops from the environmental stresses. Here we claim a potent endophyte isolated from Sonchus asper L. The aim was to explore the stress adaptive mechanism of sunflower and soybean mediated by Aspergillus niger (SonchL-7) under high temperature. Inoculation with A. niger boosted plant height, biomass and chlorophyl contents, while significantly curtailed the concentration of lipid peroxidation and reactive oxygen species (ROS) under thermal stress at 40°C. Moreover, the ROS-scavenging activities, like ascorbic acid oxidase (AAO), catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD) and peroxidase (POD) were augmented. Also, proline and phenolics were enhanced in the tested crops, while ABA concentration was significantly reduced. These positive results suggested that A. niger can be used as a heat-stress ameliorative tool for crops in the future.
Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; In-Jung Lee. Aspergillus niger boosted heat stress tolerance in sunflower and soybean via regulating their metabolic and antioxidant system. Journal of Plant Interactions 2020, 15, 223 -232.
AMA StyleIsmail, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Sumera Afzal Khan, In-Jung Lee. Aspergillus niger boosted heat stress tolerance in sunflower and soybean via regulating their metabolic and antioxidant system. Journal of Plant Interactions. 2020; 15 (1):223-232.
Chicago/Turabian StyleIsmail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; In-Jung Lee. 2020. "Aspergillus niger boosted heat stress tolerance in sunflower and soybean via regulating their metabolic and antioxidant system." Journal of Plant Interactions 15, no. 1: 223-232.
Plants are multicellular organism composed of different types of cells. These all kinds of cells are formed from pluripotent stem cells present at different positions in plant called stem cell niches. All these stem cell niches and their boundaries are maintained by complex regulatory mechanism at molecular level involving different genes, cofactors, and phytohormones. In this chapter, we discussed the regulatory mechanism and models of stem cell maintenance, specifying their boundaries at different stem cell niches.
Aqib Syed; Anwar Hussain; Waheed Murad; Badshah Islam. Regulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell Niches. Methods in Molecular Biology 2019, 2094, 1 -16.
AMA StyleAqib Syed, Anwar Hussain, Waheed Murad, Badshah Islam. Regulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell Niches. Methods in Molecular Biology. 2019; 2094 ():1-16.
Chicago/Turabian StyleAqib Syed; Anwar Hussain; Waheed Murad; Badshah Islam. 2019. "Regulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell Niches." Methods in Molecular Biology 2094, no. : 1-16.