Natural products from plants, such as flavonoids, arouse immense interest in medicine because of the therapeutic and many other bioactive properties. The molecular docking is a very useful method to screen the molecules based on their free binding energies and give important structural suggestions about how molecules might activate or inhibit the target receptor by comparing reference molecules. Alliin and Allicin differ from many other flavonoids because of containing no benzene rings and having nitrogen and sulfur atoms in their structure. In this study Alliin and Allicin affinity on AMPA, NMDA and GABA-A receptors were evaluated in the central nervous system by using the molecular docking method. Both Alliin and Allicin indicated no inhibitory effects. However Alliin showed significant selectivity to human AMPA receptor (3RN8) as an excitatory. The binding energy of glutamate to 3RN8 was −6.61 kcal mol ⁻¹ , while the binding energy of Allin was −8.08 kcal mol ⁻¹ . Furthermore Alliin’s affinity to the other AMPA and NMDA receptors is quite satisfactory compared to the reference molecule glutamate. In conclusion based on the molecular docking study, Alliin can be useful for synaptic plasticity studies whereas might be enhance seizure activity because of the increased permeability to cations. It also can be beneficial to improve learning and memory and can be used as a supportive product to the hypofunction of NMDA associated problems.

The development of smart, selective, and multifunctional nanotherapeutics is crucial for advancing next-generation cancer treatments. In this study, superparamagnetic iron oxide nanoclusters (SPIONCs) were coated with mesoporous silica, functionalized with folic acid (FA), and co-loaded with curcumin (CUR) and capecitabine (CAPE) to create a novel nanocarrier system. To enhance cellular internalisation, magnetophoresis was applied before exposure of the cells to cold atmospheric plasma (CAP). The resulting FA-conjugated, CUR and CAPE-loaded nanoclusters were evaluated in vitro in MCF-7 breast cancer and HME-1 normal epithelial cells at varying CAP exposure durations (0, 10, and 20 s) and incubation times (24 and 48 h). This is the first report demonstrating the co-loading of CUR and CAPE into FA-functionalised mesoporous silica-coated magnetic nanoclusters. Drug release studies revealed significantly enhanced release profiles under acidic conditions (pH 5.0 and 6.5), mimicking lysosomal and tumour microenvironments, compared to physiological pH (7.4). Drug-loaded nanoclusters exhibited substantially higher cytotoxicity than the controls with no loading, with a more pronounced effect in MCF-7 cells. Notably, the combined treatment of CAP and CUR-CAPE loaded NCs showed a synergistic cytotoxic effect. IC₅₀ values, after 10 s CAP exposure and 24 h incubation, decreased to 0.43 µg mL⁻¹ for MCF-7 cells and 37 µg mL⁻¹ for HME-1 cells. The elevated levels of reactive oxygen species (ROS) induced by CAP played a key role in the observed cytotoxic effects, and both CUR and CAPE were found to enhance this process through ROS-related and potentially additional molecular pathways. These findings highlight the potential of CAP-assisted multicomponent nanocarriers as a promising platform for effective cancer therapy.

Background: The most common polyphenolic compounds taken up by the organism through the food chain are flavonoids. Known to cross the blood-brain barrier, 7,8-DHF, L-theanine and Fisetin are re-ported to have neuronal effects as well as therapeutic potential for neurodegenerative diseases. How-ever, little is known on their acute effects of neuronal function. This study aims to describe the effects of the mentioned flavonoids on the total ECoG activities and band analyzes of healthy mice. Materials and Methods: For this purpose, 3 different groups consisting of 7 subjects were created for each flavonoid administration. After the baseline electrophysiological recordings, flavonoid administra-tion was performed and acute effects were determined. Results: 7.8-DHF increased the theta, alpha and beta band activities while decreasing the total ECoG power. L-theanine and Fisetin did not significantly alter the total ECoG activity. However, L-theanine statistically increased theta, alpha and beta band activities. Conclusions: In conclusion, our data showed that flavonoids could acutely modulate the ECoG respons-es in a band specific manner. They can be considered as candidate molecules for drug discovery studies for central nervous system disorders.