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Discussion
11 December 2023

Investigating Antibody Reactivity to the Intestinal Microbiome in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): A Feasibility Study

microbiome
immunoglobulin A
immunoglobulin G
immune tolerance
myalgic encephalomyeli...
Related publication: 10.3390/ijms242015316
Loooks like the data are compromised by the triggering on sidescatter well into the population of bacteria. (Figre 2 and 4) compromising all the numerical data.

On the Sony cell sorter the trigger goes into the instrument noise as indicatied by the levelling off of the back scatter height data vs backscatter area.

The regions shown are inadequate going across population boundaries raising serious questions about the quality of the work and the sorting.
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