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Dr. Mustafa Shukry
Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt

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0 Animal Physiology
0 Cell Biology
0 Molecular Biology
0 Fish physiology and nutrition
0 Physiology of farm animals

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Journal article
Published: 18 August 2021 in Animals
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The present study aimed to assess the in ovo administration of clenbuterol on chick fertility, growth performance, muscle growth, myogenic gene expression, fatty acid, amino acid profile, intestinal morphology, and hepatic lipid-related gene expressions. In this study, 750 healthy fertile eggs from the local chicken breed Dokki-4 strain were analyzed. Fertile eggs were randomly divided into five experimental groups (150 eggs/3 replicates for each group). On day 14 of incubation, in addition to the control group, four other groups were established where 0.5 mL of worm saline (30 °C) was injected into the second group of eggs. In the third, fourth, and fifth groups, 0.5 mL of worm saline (30 °C), 0.9% of NaCl, and 10, 15, and 20 ppm of clenbuterol were injected into the eggs. Results suggested that clenbuterol increased growth efficiency up to 12 weeks of age, especially at 15 ppm, followed by 10 ppm, decreased abdominal body fat mass, and improved hatchability (p< 0.01). Clenbuterol also modulated saturated fatty acid levels in the breast muscles and improved essential amino acids when administered at 10 and 15 ppm. Additionally, clenbuterol at 15 ppm significantly decreased myostatin gene expression (p< 0.01) and considerably increased IGF1r and IGF-binding protein (IGFBP) expression. Clenbuterol administration led to a significant upregulation of hepatic PPARα, growth hormone receptor, and Lipoprotein lipase (LPL) mRNA expression with a marked decrease in fatty acid synthase (FAS) and sterol regulatory element-binding protein 1 (SREBP-1c) expression. In conclusion, the current study revealed that in ovo injection of clenbuterol showed positive effects on the growth of hatched chicks through reduced abdominal fat deposition, improved intestinal morphology, and modulation of hepatic gene expressions in myogenesis, lipogenesis, and lipolysis.

ACS Style

Ahmed A. Saleh; Rashed A. Alhotan; Abdulrahman S. Alharthi; Eldsokey Nassef; Mohamed A. Kassab; Foad A. Farrag; Basma M. Hendam; Mohamed M. A. Abumnadour; Mustafa Shukry. Insight View on the Role of in Ovo Feeding of Clenbuterol on Hatched Chicks: Hatchability, Growth Efficiency, Serum Metabolic Profile, Muscle, and Lipid-Related Markers. Animals 2021, 11, 2429 .

AMA Style

Ahmed A. Saleh, Rashed A. Alhotan, Abdulrahman S. Alharthi, Eldsokey Nassef, Mohamed A. Kassab, Foad A. Farrag, Basma M. Hendam, Mohamed M. A. Abumnadour, Mustafa Shukry. Insight View on the Role of in Ovo Feeding of Clenbuterol on Hatched Chicks: Hatchability, Growth Efficiency, Serum Metabolic Profile, Muscle, and Lipid-Related Markers. Animals. 2021; 11 (8):2429.

Chicago/Turabian Style

Ahmed A. Saleh; Rashed A. Alhotan; Abdulrahman S. Alharthi; Eldsokey Nassef; Mohamed A. Kassab; Foad A. Farrag; Basma M. Hendam; Mohamed M. A. Abumnadour; Mustafa Shukry. 2021. "Insight View on the Role of in Ovo Feeding of Clenbuterol on Hatched Chicks: Hatchability, Growth Efficiency, Serum Metabolic Profile, Muscle, and Lipid-Related Markers." Animals 11, no. 8: 2429.

Journal article
Published: 25 June 2021 in International Journal of Molecular Sciences
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D-galactose (D-gal) administration causes oxidative disorder and is widely utilized in aging animal models. Therefore, we subcutaneously injected D-gal at 200 mg/kg BW dose to assess the potential preventive effect of thymoquinone (TQ) and curcumin (Cur) against the oxidative alterations induced by D-gal. Other than the control, vehicle, and D-gal groups, the TQ and Cur treated groups were orally supplemented at 20 mg/kg BW of each alone or combined. TQ and Cur effectively suppressed the oxidative alterations induced by D-gal in brain and heart tissues. The TQ and Cur combination significantly decreased the elevated necrosis in the brain and heart by D-gal. It significantly reduced brain caspase 3, calbindin, and calcium-binding adapter molecule 1 (IBA1), heart caspase 3, and BCL2. Expression of mRNA of the brain and heart TP53, p21, Bax, and CASP-3 were significantly downregulated in the TQ and Cur combination group along with upregulation of BCL2 in comparison with the D-gal group. Data suggested that the TQ and Cur combination is a promising approach in aging prevention.

ACS Style

Ali El-Far; Yaser Elewa; Elsayeda-Zeinab Abdelfattah; Abdel-Wahab Alsenosy; Mustafa Atta; Khalid Abou-Zeid; Soad Al Jaouni; Shaker Mousa; Ahmed Noreldin. Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart. International Journal of Molecular Sciences 2021, 22, 6839 .

AMA Style

Ali El-Far, Yaser Elewa, Elsayeda-Zeinab Abdelfattah, Abdel-Wahab Alsenosy, Mustafa Atta, Khalid Abou-Zeid, Soad Al Jaouni, Shaker Mousa, Ahmed Noreldin. Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart. International Journal of Molecular Sciences. 2021; 22 (13):6839.

Chicago/Turabian Style

Ali El-Far; Yaser Elewa; Elsayeda-Zeinab Abdelfattah; Abdel-Wahab Alsenosy; Mustafa Atta; Khalid Abou-Zeid; Soad Al Jaouni; Shaker Mousa; Ahmed Noreldin. 2021. "Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart." International Journal of Molecular Sciences 22, no. 13: 6839.

Journal article
Published: 31 May 2021 in Animals
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Nile tilapia Juveniles (19.50 ± 0.5 g) were fed on a basal diet (control group (CTR)) and a diet supplemented with 1 g Aspergillus oryzae (ASP) per kg diet for 12 weeks. Fish were then subjected to different salinity levels (0, 10, 15, and 20 practical salinity units (psu)) for another 15 days. Two-way ANOVA analysis revealed that the individual effects of ASP in Nile tilapia exposed to salinity levels presented a significant decrease (p< 0.05) in values of haemato-biochemical indices (such as glucose, cortisol, alanine transaminase, aspartate transaminase, and malondialdehyde) compared to those in the CTR group exposed to the same salinity levels. Moreover, significant increases (p< 0.05) of blood protein profile (albumin, globulin, and total protein), non-specific immune responses (lysozyme activity, phagocytic activity, and phagocytic index), and antioxidant enzymes activities (glutathione peroxidase, catalase, and superoxide dismutase) were observed in ASP-supplemented groups. Interestingly, there was significant (p< 0.05) downregulation of the mRNA expression values of heat shock protein 70 and interferon-gamma genes, alongside upregulation of the mRNA expression values of interleukin 1 beta and interleukin 8 genes, in the hepatic tissues of Nile tilapia in ASP-supplemented groups exposed to different salinities compared to those in the CTR group exposed to the same salinity levels. Taken together, these findings supported the potential efficacy of dietary supplementation with ASP in alleviating salinity stress-induced haemato-biochemical alterations, immune suppression, and oxidative stress in the exposed Nile tilapia.

ACS Style

Mustafa Shukry; Marwa Abd El-Kader; Basma Hendam; Mahmoud Dawood; Foad Farrag; Salama Aboelenin; Mohamed Soliman; Hany Abdel-Latif. Dietary Aspergillus oryzae Modulates Serum Biochemical Indices, Immune Responses, Oxidative Stress, and Transcription of HSP70 and Cytokine Genes in Nile Tilapia Exposed to Salinity Stress. Animals 2021, 11, 1621 .

AMA Style

Mustafa Shukry, Marwa Abd El-Kader, Basma Hendam, Mahmoud Dawood, Foad Farrag, Salama Aboelenin, Mohamed Soliman, Hany Abdel-Latif. Dietary Aspergillus oryzae Modulates Serum Biochemical Indices, Immune Responses, Oxidative Stress, and Transcription of HSP70 and Cytokine Genes in Nile Tilapia Exposed to Salinity Stress. Animals. 2021; 11 (6):1621.

Chicago/Turabian Style

Mustafa Shukry; Marwa Abd El-Kader; Basma Hendam; Mahmoud Dawood; Foad Farrag; Salama Aboelenin; Mohamed Soliman; Hany Abdel-Latif. 2021. "Dietary Aspergillus oryzae Modulates Serum Biochemical Indices, Immune Responses, Oxidative Stress, and Transcription of HSP70 and Cytokine Genes in Nile Tilapia Exposed to Salinity Stress." Animals 11, no. 6: 1621.

Journal article
Published: 11 May 2021 in Animals
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The existing study was designed to assess the influences of dietary activated charcoal (AC) on the growth performance, immune responses, antioxidative status, and its mitigating roles against the physiological responses of Nile tilapia exposed a sub-lethal dose of a neonicotinoid agriculture pesticide, namely, as imidacloprid (IMID). Nile tilapia juveniles were fed on diets supplemented with graded AC levels as 0 (control), 5, 10, 15, and 20 g/kg diet for eight weeks. Growth, hemato-biochemical indices, and antioxidant and immune responses of fish in all groups were evaluated at the end of the feeding experiment. Afterward, fish in all experimental groups were subjected to a sub-lethal dose of IMID (0.0109 μg/L) for two weeks. Then, fish mortalities, stress indicators, and IMID residual levels in liver and flesh were examined. Results of the feeding experiment showed that total feed intake, weight gain, final body weights, and feed efficiency ratio were significantly increased in all AC groups compared with the control group. The survival rate was 100% in all experimental groups. No statistical differences were observed in the hematological picture of all experimental groups except the lymphocyte count, which was significantly increased in all AC groups compared to the control group. Total protein, albumin, globulin, nitric oxide levels, lysozyme, and respiratory burst activities were significantly increased in all AC groups. Serum alanine transaminase, aspartate transaminase, alkaline phosphatase activities, and malondialdehyde (MDA) levels were significantly decreased in all AC groups compared with the AC0 group. After exposure to a sub-lethal dose of IMID, survival rates were significantly elevated, and IMID residual levels in liver and flesh were significantly decreased in all AC groups than in the control group. Moreover, second-order polynomial regression showed that dietary supplementation with 14.30 g AC/kg diet resulted in the lowest blood glucose and serum MDA levels. Conclusively, we suggest dietary supplementation with 14.30 g AC/kg diet to modulate physiological responses of Nile tilapia to sub-lethal IMID toxicity.

ACS Style

Samah Abd El-Hameed; Samar Negm; Nahla Ismael; Mohammed Naiel; Mohamed Soliman; Mustafa Shukry; Hany Abdel-Latif. Effects of Activated Charcoal on Growth, Immunity, Oxidative Stress Markers, and Physiological Responses of Nile Tilapia Exposed to Sub-Lethal Imidacloprid Toxicity. Animals 2021, 11, 1357 .

AMA Style

Samah Abd El-Hameed, Samar Negm, Nahla Ismael, Mohammed Naiel, Mohamed Soliman, Mustafa Shukry, Hany Abdel-Latif. Effects of Activated Charcoal on Growth, Immunity, Oxidative Stress Markers, and Physiological Responses of Nile Tilapia Exposed to Sub-Lethal Imidacloprid Toxicity. Animals. 2021; 11 (5):1357.

Chicago/Turabian Style

Samah Abd El-Hameed; Samar Negm; Nahla Ismael; Mohammed Naiel; Mohamed Soliman; Mustafa Shukry; Hany Abdel-Latif. 2021. "Effects of Activated Charcoal on Growth, Immunity, Oxidative Stress Markers, and Physiological Responses of Nile Tilapia Exposed to Sub-Lethal Imidacloprid Toxicity." Animals 11, no. 5: 1357.

Journal article
Published: 07 May 2021 in Sustainability
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As the world population increases, there will be an increase in demand for poultry meat. To satisfy this requirement, sustainably will be a significant challenge as different countries in the world face different health problems. Additionally, for decades, poultry nutritionists have worked on sustainability in broiler manufacturing plants. The usage of short- and medium-chain fatty acids (MCFAs), which have positive effects as growth promoters and immunostimulants, is a pioneering way to decrease the cost of diets and improve environmentally friendly and sustainable production in this critical era. This study aimed at evaluating the influences of alpha-monoglyceride (alpha-monolaurin) on the growth performance, biochemical parameters, lipid profiles, liver oxidative stress, and immune response in broilers. Alpha-monolaurin was included at the levels of 0 (control), 0.25, 0.5, and 1 g/kg in broiler diets. After 33 days, dietary supplementation of alpha-monolaurin showed no impact on the feed intake; however, broilers fed 0.5 g/kg feed displayed a significantly reduced food conversion ratio, as compared to the control (p ≤ 0.05). However, body weight and body weight gain tended to increase after 0.25 g/kg alpha-monolaurin supplementation, as compared to other groups. The highest European production efficiency factor (EPEF) was obtained with 0.25 g/kg alpha-monolaurin supplementation. The ether extract utilization and spleen weight were improved substantially (p ≤ 0.05) by alpha-monolaurin supplementation, but muscle and other organ weights were not affected. Plasma alanine transaminase (ALT) was decreased significantly by alpha-monolaurin supplementation, while plasma total protein and albumin were enhanced (p ≤ 0.05). Interestingly, broilers fed alpha-monolaurin displayed an increased antibody titer of the Newcastle disease (ND) virus and muscle unsaturated fatty acid content, and a decreased liver content of malondialdehyde (MDA). It could be assumed that dietary supplementation of alpha-monolaurin positively impacts birds’ feed efficiency and immune response and can be applied as a natural immune enhancer in the broiler industry.

ACS Style

Ahmed Saleh; Bahaa El-Gharabawy; Aziza Hassan; Nemat Badawi; Yahya Eid; Shaimaa Selim; Mustafa Shukry; Mahmoud Dawood. Effect of Dietary Inclusion of Alpha-Monolaurin on the Growth Performance, Lipid Peroxidation, and Immunity Response in Broilers. Sustainability 2021, 13, 5231 .

AMA Style

Ahmed Saleh, Bahaa El-Gharabawy, Aziza Hassan, Nemat Badawi, Yahya Eid, Shaimaa Selim, Mustafa Shukry, Mahmoud Dawood. Effect of Dietary Inclusion of Alpha-Monolaurin on the Growth Performance, Lipid Peroxidation, and Immunity Response in Broilers. Sustainability. 2021; 13 (9):5231.

Chicago/Turabian Style

Ahmed Saleh; Bahaa El-Gharabawy; Aziza Hassan; Nemat Badawi; Yahya Eid; Shaimaa Selim; Mustafa Shukry; Mahmoud Dawood. 2021. "Effect of Dietary Inclusion of Alpha-Monolaurin on the Growth Performance, Lipid Peroxidation, and Immunity Response in Broilers." Sustainability 13, no. 9: 5231.

Original article
Published: 06 May 2021 in Veterinary Medicine and Science
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Background Fish farming is one of the most productive economies in the world. One of the essential goals in fish production is to minimize processing costs while maintaining and increasing the vital functions, weight and immunity of fish. Objective We conducted this study to explore nanoselenium (Nano‐Se) particles in various feeding schemes. Material and Method Nano‐Se particles incorporated in the basal diet at (0.5 mg/kg diet), and the fish was divided into six groups after adaptation as the follows: The first group was feed daily with a diet containing Nano‐Se (0.5 mg/kg diet); the second group was exposed to a feeding programme in which it has day feeding followed by day of starvation with a diet containing Nano‐Se (0.5 mg/kg diet); the third group was day feeding followed by 2 days of starvation; the fourth group served as a negative control group in which this group was continuous feeding with a basal diet without Nano‐Se; the fifth group was day feeding with the basal diet followed by a day of starvation; and the sixth group was day feeding with basal diet followed by 2 days of starvation. Result Our result revealed that Group 2 showed significant improvement in haematological parameters, red blood cells and haemoglobin with a substantial increase in total protein (p < 0.05) as well as lysosomal and phagocytic activity with considerable upregulation of growth hormone and insulin growth factor 1 in addition to markedly increase in the pro‐inflammatory cytokines. Finally, this study offers the first‐time dietary regime with Nano‐Se supplementation that saves the feeding cost and increases fish welfare and growth.

ACS Style

Eman M. Moustafa; Marwa F. Abd El‐Kader; Montaser M. Hassan; Ahmed F. Fath El‐Bab; Amira Omar; Foad Farrag; Ahmed G. Gewida; Mohamed F. Abd‐Elghany; Mustafa Shukry; Rasha A. Alwakeel. Trial for use nanoselenium particle with different dietary regime in Oreochromis niloticus and Mugil cephalus polyculture ponds: Growth efficiency, haematological, antioxidant, immunity and transcriptional analysis. Veterinary Medicine and Science 2021, 1 .

AMA Style

Eman M. Moustafa, Marwa F. Abd El‐Kader, Montaser M. Hassan, Ahmed F. Fath El‐Bab, Amira Omar, Foad Farrag, Ahmed G. Gewida, Mohamed F. Abd‐Elghany, Mustafa Shukry, Rasha A. Alwakeel. Trial for use nanoselenium particle with different dietary regime in Oreochromis niloticus and Mugil cephalus polyculture ponds: Growth efficiency, haematological, antioxidant, immunity and transcriptional analysis. Veterinary Medicine and Science. 2021; ():1.

Chicago/Turabian Style

Eman M. Moustafa; Marwa F. Abd El‐Kader; Montaser M. Hassan; Ahmed F. Fath El‐Bab; Amira Omar; Foad Farrag; Ahmed G. Gewida; Mohamed F. Abd‐Elghany; Mustafa Shukry; Rasha A. Alwakeel. 2021. "Trial for use nanoselenium particle with different dietary regime in Oreochromis niloticus and Mugil cephalus polyculture ponds: Growth efficiency, haematological, antioxidant, immunity and transcriptional analysis." Veterinary Medicine and Science , no. : 1.

Journal article
Published: 30 April 2021 in Animals
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The study investigated the ability of boswellic acid (BA) to alleviate the testicular and oxidative injury FPN insecticide intoxication in the male rat model. Rats were randomly assigned to six equivalent groups (six rats each) as the following: control rats orally administered with 2 mL physiological saline/kg of body weight (bwt); boswellic acid (BA1) rats orally administered 250 mg BA/kg bwt; boswellic acid (BA2) rats orally administered 500 mg BA/kg bwt; fipronil (FPN) rats orally administered 20 mg FPN/kg bwt; (FPN + BA1) rats orally administered 20 mg FPN/kg bwt plus 250 mg BA/kg bwt, and (FPN + BA2) rats orally administered 20 mg FPN/kg bwt plus 500 mg BA/kg bwt. After 60 days, semen viability percentage and live spermatozoa percentage were decreased, and a considerably increased abnormality of the sperm cells in FPN-administered rats improved substantially with the co-administration of BA. BA had refinement of the histological architecture of testes and sexual glands. Quantitative analysis recorded a noticeable decline in the nuclear cell-proliferating antigen (PCNA) percentage area. FPN triggered cell damage, which was suggested by elevated malondialdehyde and interleukin 6, tumor necrosis factors alpha, and decreased glutathione level. Proapoptotic factor overexpression is mediated by FPN administration, while it decreased the antiapoptotic protein expression. Similarly, BA has shown significant upregulation in steroidogenic and fertility-related gene expression concerning the FPN group. Pathophysiological damages induced by FPN could be alleviated by BA’s antioxidant ability and antiapoptotic factor alongside the upregulation of steroidogenic and fertility-related genes and regimented the detrimental effects of FPN on antioxidant and pro-inflammatory biomarkers.

ACS Style

Hossam Tohamy; Sara El-Kazaz; Saqer Alotaibi; Hawary Ibrahiem; Mustafa Shukry; Mahmoud Dawood. Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats. Animals 2021, 11, 1302 .

AMA Style

Hossam Tohamy, Sara El-Kazaz, Saqer Alotaibi, Hawary Ibrahiem, Mustafa Shukry, Mahmoud Dawood. Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats. Animals. 2021; 11 (5):1302.

Chicago/Turabian Style

Hossam Tohamy; Sara El-Kazaz; Saqer Alotaibi; Hawary Ibrahiem; Mustafa Shukry; Mahmoud Dawood. 2021. "Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats." Animals 11, no. 5: 1302.

Journal article
Published: 18 April 2021 in Sustainability
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Natural and chemical colorants are attracting a lot of attention as sustainable feed additives due to their effect on food color and because presentation matters to consumers. Color also is a major sensory factor that helps consumers determine food quality and its possible health benefits. For example, highly colored egg yolks can only come from healthy laying hens fed a well-balanced, nutrient-rich diet. Consumers associate an intense yolk color with healthy, nutrient-rich food. There is a growing market request for eggs with rich yolk coloring. The purpose of the current study was to evaluate the impact of natural (paprika) and chemical (carmoisine) colorant supplementation on the performance, egg-quality characteristics, yolk fatty-acid profile, and blood constituents in laying hens. A total of 240 Bovans laying hens were randomly distributed in a completely randomized design in four treatments according to four experimental diets: a control diet (without supplementation of any colorants), a control diet supplemented with 4 kg/ton paprika, a control diet supplemented with 150 g carmoisine/ton, and a control diet supplemented with a combination of 4 kg/ton paprika plus 150 g carmoisine/ton, fed from 42 to 54 weeks of age. Each treatment consisted of 10 replications with six hens. Dietary inclusion of paprika colorant improved (p < 0.05) the feed-conversion ratio and egg-production rate, while final body weight, weight change, feed intake, and egg weight and mass were not affected. The degrees of egg yolk and white were increased (p < 0.05) by the dietary supplements. Yolk color and shell thickness were enhanced (p < 0.05) by adding paprika and/or carmoisine colorants. Supplementation of the paprika colorant alone or in combination with carmoisine increased (p < 0.05) linolenic acid, oleic acid, and vitamin E concentrations in egg yolks. In contrast, egg yolk palmitic acid and liver malondialdehyde contents were decreased (p < 0.05). Paprika and carmoisine colorants and their combination improved (p < 0.05) blood lipid profile in treated hens. We concluded that the dietary supplementation of natural (paprika) colorants has an influential role in improving egg yolk color, production performance, and egg yolk fatty-acid profile in laying hens.

ACS Style

Ahmed A. Saleh; Esraa Gawish; Samy F. Mahmoud; Khairy Amber; Wael Awad; Mohammed H. Alzawqari; Mustafa Shukry; Abdel-Moneim Eid Abdel-Moneim. Effect of Natural and Chemical Colorant Supplementation on Performance, Egg-Quality Characteristics, Yolk Fatty-Acid Profile, and Blood Constituents in Laying Hens. Sustainability 2021, 13, 4503 .

AMA Style

Ahmed A. Saleh, Esraa Gawish, Samy F. Mahmoud, Khairy Amber, Wael Awad, Mohammed H. Alzawqari, Mustafa Shukry, Abdel-Moneim Eid Abdel-Moneim. Effect of Natural and Chemical Colorant Supplementation on Performance, Egg-Quality Characteristics, Yolk Fatty-Acid Profile, and Blood Constituents in Laying Hens. Sustainability. 2021; 13 (8):4503.

Chicago/Turabian Style

Ahmed A. Saleh; Esraa Gawish; Samy F. Mahmoud; Khairy Amber; Wael Awad; Mohammed H. Alzawqari; Mustafa Shukry; Abdel-Moneim Eid Abdel-Moneim. 2021. "Effect of Natural and Chemical Colorant Supplementation on Performance, Egg-Quality Characteristics, Yolk Fatty-Acid Profile, and Blood Constituents in Laying Hens." Sustainability 13, no. 8: 4503.

Journal article
Published: 05 April 2021 in Animals
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This experiment was conducted to assess the inclusion of the by-products of pomegranate peels to ameliorate the harmful impacts of oxidative stress in the pre-peak period of laying hens. For this, 120 local Egyptian strain hens (Inshas hens) aged 24 weeks old were used in four treatments. Pomegranate peel powder was included at 2% and 4% in the diets of laying hens subjected to oxidative injuries induced by dexamethasone compared with negative and positive control groups for 12 weeks. The addition of pomegranate peel powder (PPP) reduced the adverse effects of oxidative stress induced by dexamethasone on body weight (p = 0.006) and egg production (p = 0.010) comparing to the positive control. Additionally, pomegranate peel powder had a significant positive lowering effect on plasma cholesterol (p < 0.001) and triglyceride contents (p = 0.005) compared to control groups. The lipid peroxidation indicators (MDA) were reduced, but the antioxidative enzymes (SOD, CAT, and GPx) and total antioxidant blood capacity were improved with PPP. Based on the obtained data, the present research recommends using dietary PPP up to 4% to mitigate adverse oxidative stress effects in the pre-peak laying period and as a sustainable and economical approach for agricultural development.

ACS Style

Yahya Eid; Abeer Kirrella; Ahmed Tolba; Maged El-Deeb; Samy Sayed; Hanan El-Sawy; Mustafa Shukry; Mahmoud Dawood. Dietary Pomegranate By-Product Alleviated the Oxidative Stress Induced by Dexamethasone in Laying Hens in the Pre-Peak Period. Animals 2021, 11, 1022 .

AMA Style

Yahya Eid, Abeer Kirrella, Ahmed Tolba, Maged El-Deeb, Samy Sayed, Hanan El-Sawy, Mustafa Shukry, Mahmoud Dawood. Dietary Pomegranate By-Product Alleviated the Oxidative Stress Induced by Dexamethasone in Laying Hens in the Pre-Peak Period. Animals. 2021; 11 (4):1022.

Chicago/Turabian Style

Yahya Eid; Abeer Kirrella; Ahmed Tolba; Maged El-Deeb; Samy Sayed; Hanan El-Sawy; Mustafa Shukry; Mahmoud Dawood. 2021. "Dietary Pomegranate By-Product Alleviated the Oxidative Stress Induced by Dexamethasone in Laying Hens in the Pre-Peak Period." Animals 11, no. 4: 1022.

Original article
Published: 24 March 2021 in Aquaculture Research
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The current study was designed to evaluate the ameliorative effect of lipopolysaccharides (LPS) (a portion of the Gram –ve bacteria cell wall) on aflatoxin (AFB1)‐persuaded changes in the cytokine expression, heat‐shock protein 70 (HSP70), antioxidant genes, different haematological and biochemical parameters in Nile Tilapia along with the infection by Aeromonas hydrophila and the immune‐stimulant efficiency of LPS on the growth pattern. For this study, two hundred and forty O. niloticus (35 ± 1.5 g) were used. The fish were randomly allocated into four experimental groups, four replicates for each group (15 fish/replicate). Group 1 was the negative control group fed on a basal diet, and group 2 was the positive control group fed on the basal diet with aflatoxins (AFB1) 200 µg/kg feed. Group 3 has fed the lipopolysaccharides 20 mg/100 gm diets. Group 4, besides the AFB1‐contaminated basal diet, was provided with lipopolysaccharides 20 mg/100 gm diet. At the end of the 12 weeks, each group was injected with A. hydrophila. The analysis revealed that LPS‐treated AFB1‐supplemented fish showed improvement in haematological, lysosomal and phagocytic activities and total protein and significantly decreased liver enzyme exaggerated by aflatoxin; alongside, the LPS‐treated aflatoxin‐supplemented fish revealed a significant effect on the haematological and the immunity markers after 24 h, 48 h and 1 week post challenged with A. hydrophila. Concerning the gene expression, AFB1 group showed significant up‐regulation of HSP70 and interferon gamma gene mRNA expression, which is significantly down‐regulated by LPS treatment, besides LPS showed up‐regulation of catalase and superoxide dismutase genes markedly alongside with interleukins (IL), IL‐8 and IL‐1B; the binding effect of LPS markedly appears after A. hydrophila which significantly modulates the HSP70 and immunity marker genes in addition to the antioxidant‐related genes, CAT and SOD. Histopathological findings reflected a marked increase in intestinal villi length in LPS‐treated groups. Our view illuminates for the first time the pathways of molecular wellness for the role of LPS in AFB1 toxicity and bacterial infection. Our observation illuminates the molecular fitness pathways for the LPS that can aide to the development of a bacterial disease tolerance strategy and potentially boost immune response in O. niloticus fish.

ACS Style

Marwa F. Abd El‐Kader; Mustafa Shukry; Mahmoud A. O. Dawood; Mohamed M. Hassan; Foad Farrag; Amira Omar; Hanan B. El‐Sawy; Mohamed A. Abd Alaziz; Hazem S. Hamouda; Mohamed F. Abd ‐Elghany; Eman M. Moustafa. Ameliorative effect of dietary lipopolysaccharides on Oreochromis niloticus juveniles submitted to aflatoxin B1‐induced oxidative stress and bacterial challenge. Aquaculture Research 2021, 1 .

AMA Style

Marwa F. Abd El‐Kader, Mustafa Shukry, Mahmoud A. O. Dawood, Mohamed M. Hassan, Foad Farrag, Amira Omar, Hanan B. El‐Sawy, Mohamed A. Abd Alaziz, Hazem S. Hamouda, Mohamed F. Abd ‐Elghany, Eman M. Moustafa. Ameliorative effect of dietary lipopolysaccharides on Oreochromis niloticus juveniles submitted to aflatoxin B1‐induced oxidative stress and bacterial challenge. Aquaculture Research. 2021; ():1.

Chicago/Turabian Style

Marwa F. Abd El‐Kader; Mustafa Shukry; Mahmoud A. O. Dawood; Mohamed M. Hassan; Foad Farrag; Amira Omar; Hanan B. El‐Sawy; Mohamed A. Abd Alaziz; Hazem S. Hamouda; Mohamed F. Abd ‐Elghany; Eman M. Moustafa. 2021. "Ameliorative effect of dietary lipopolysaccharides on Oreochromis niloticus juveniles submitted to aflatoxin B1‐induced oxidative stress and bacterial challenge." Aquaculture Research , no. : 1.

Journal article
Published: 23 March 2021 in Sustainability
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The practical usage of untraditional feedstuffs such as sunflower meal (SFM) in laying hens nutrition in developing countries has received considerable attention. SFM is a by-product of the sunflower oil industry and has been progressively added to bird’s diets. Sunflower meal (SFM) is gaining great interest as a feed ingredient due to its eminent crude protein content, low anti-nutritional compounds, and low price. The current experiment was aimed to assess the production efficiency, egg quality, yolk fatty acids composition, and nutrient digestibility of laying hens fed SFM. A total of 162 Bovans Brown laying hens aged 60 weeks old were randomly allocated using a completely randomized design into three experimental groups of nine replicates each (n = six/replicate) for eight weeks. The dietary treatments involved a control (basal diet) and two levels of SFM, 50 and 100 g/kg feed. The dietary treatments did not influence live weight gain, feed intake, and egg mass. On one hand, the laying rate was increased; on the other hand, the feed conversion ratio and broken eggs rate of laying hens were decreased (p < 0.05) by the dietary inclusion of SFM. Dietary treatments had no effect on the egg’s quality characteristics except the yolk color and yolk height were larger (p = 0.01) for laying hens fed SFM compared with those fed the control. Dietary inclusion of SFM decreased (p < 0.05) the content of cholesterol in the egg yolk. Still, it increased the yolk contents of vitamin E, calcium, linoleic acid, linolenic acid, and oleic acid (p < 0.05). Furthermore, the dietary inclusion of SFM increased crude protein and calcium digestibility, but decreased the ether extract digestibility. In conclusion, our results suggested that the dietary inclusion of SFM, up to 100 g/kg at a late phase of laying, could improve the production performance, some of the egg quality traits, and nutrient digestibility while decreasing egg yolk cholesterol.

ACS Style

Ahmed Saleh; Ahmed El-Awady; Khairy Amber; Yahya Eid; Mohammed Alzawqari; Shaimaa Selim; Mohamed Soliman; Mustafa Shukry. Effects of Sunflower Meal Supplementation as a Complementary Protein Source in the Laying Hen’s Diet on Productive Performance, Egg Quality, and Nutrient Digestibility. Sustainability 2021, 13, 3557 .

AMA Style

Ahmed Saleh, Ahmed El-Awady, Khairy Amber, Yahya Eid, Mohammed Alzawqari, Shaimaa Selim, Mohamed Soliman, Mustafa Shukry. Effects of Sunflower Meal Supplementation as a Complementary Protein Source in the Laying Hen’s Diet on Productive Performance, Egg Quality, and Nutrient Digestibility. Sustainability. 2021; 13 (6):3557.

Chicago/Turabian Style

Ahmed Saleh; Ahmed El-Awady; Khairy Amber; Yahya Eid; Mohammed Alzawqari; Shaimaa Selim; Mohamed Soliman; Mustafa Shukry. 2021. "Effects of Sunflower Meal Supplementation as a Complementary Protein Source in the Laying Hen’s Diet on Productive Performance, Egg Quality, and Nutrient Digestibility." Sustainability 13, no. 6: 3557.

Journal article
Published: 20 March 2021 in Animals
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Doxorubicin (DOX) has a potent antineoplastic efficacy and is considered a cornerstone of chemotherapy. However, it causes several dose-dependent cardiotoxic results, which has substantially restricted its clinical application. This study was intended to explore the potential ameliorative effect of date palm pollen ethanolic extract (DPPE) against DOX-induced cardiotoxicity and the mechanisms underlying it. Forty male Wistar albino rats were equally allocated into Control (CTR), DPPE (500 mg/kg bw for 4 weeks), DOX (2.5 mg/kg bw, intraperitoneally six times over 2 weeks), and DPPE + DOX-treated groups. Pre-coadministration of DPPE with DOX partially ameliorated DOX-induced cardiotoxicity as DPPE improved DOX-induced body and heart weight changes and mitigated the elevated cardiac injury markers activities of serum aminotransferases, lactate dehydrogenase, creatine kinase, and creatine kinase-cardiac type isoenzyme. Additionally, the concentration of serum cardiac troponin I (cTnI), troponin T (cTnT), N-terminal pro-brain natriuretic peptide (NT-pro BNP), and cytosolic calcium (Ca+2) were amplified. DPPE also alleviated nitrosative status (nitric oxide) in DOX-treated animals, lipid peroxidation and antioxidant molecules as glutathione content, and glutathione peroxidase, catalase, and superoxide dismutase activities and inflammatory markers levels; NF-κB p65, TNF-α, IL-1β, and IL-6. As well, it ameliorated the severity of histopathological lesions, histomorphometric alteration and improved the immune-staining of the pro-fibrotic (TGF-β1), pro-apoptotic (caspase-3 and Bax), and anti-apoptotic (Bcl-2) proteins in cardiac tissues. Collectively, pre-coadministration of DPPE partially mitigated DOX-induced cardiac injuries via its antioxidant, anti-inflammatory, anti-fibrotic, and anti-apoptotic potential.

ACS Style

Samar Elblehi; Yasser El-Sayed; Mohamed Soliman; Mustafa Shukry. Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways. Animals 2021, 11, 886 .

AMA Style

Samar Elblehi, Yasser El-Sayed, Mohamed Soliman, Mustafa Shukry. Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways. Animals. 2021; 11 (3):886.

Chicago/Turabian Style

Samar Elblehi; Yasser El-Sayed; Mohamed Soliman; Mustafa Shukry. 2021. "Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways." Animals 11, no. 3: 886.

Journal article
Published: 02 March 2021 in Biology
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The current investigation assessed the impacts of sub-lethal concentrations of silicon dioxide nanoparticles (SiO2NPs) on hepato-renal functions, histopathological characteristics, and gene transcription in gills and liver of Nile tilapia juveniles. Fish were exposed to 20, 40, and 100 mg/L of SiO2NPs for 3 weeks. Pairwise comparisons with the control group showed a significant dose-dependent elevation in serum ALP, ALT, and AST enzyme activities as well as blood urea and creatinine levels in SiO2NP-intoxicated groups. Exposure to 100 mg/L SiO2NPs significantly upregulated expression of HSP70, TNF-α, IL-1β, and IL-8 genes in the gills as compared to the control group. Moreover, exposure to 100 mg/L SiO2NPs significantly upregulated the expression SOD, HSP70, IL-1β, IL-8, and TNF-α genes in the hepatic tissues as compared to the control group. Exposure of fish to 20 mg SiO2NPs/L significantly increased the mRNA expression levels of IL-12 in both the gills and liver tissues. Notably, all tested SiO2NP concentrations significantly upregulated the transcription of CASP3 gene in gills and liver of Nile tilapia as compared to the control group. Interestingly, varying histopathological alterations in renal, hepatopancreatic, and branchial tissues were observed to be correlated to the tested SiO2NP concentrations. In conclusion, our results provide additional information on the toxic impacts of SiO2NPs in Nile tilapia at the hematological, tissue, and molecular levels.

ACS Style

Hany Abdel-Latif; Mustafa Shukry; Omnia El Euony; Mohamed Mohamed Soliman; Ahmed Noreldin; Hanan Ghetas; Mahmoud Dawood; Mohamed Khallaf. Hazardous Effects of SiO2 Nanoparticles on Liver and Kidney Functions, Histopathology Characteristics, and Transcriptomic Responses in Nile Tilapia (Oreochromis niloticus) Juveniles. Biology 2021, 10, 183 .

AMA Style

Hany Abdel-Latif, Mustafa Shukry, Omnia El Euony, Mohamed Mohamed Soliman, Ahmed Noreldin, Hanan Ghetas, Mahmoud Dawood, Mohamed Khallaf. Hazardous Effects of SiO2 Nanoparticles on Liver and Kidney Functions, Histopathology Characteristics, and Transcriptomic Responses in Nile Tilapia (Oreochromis niloticus) Juveniles. Biology. 2021; 10 (3):183.

Chicago/Turabian Style

Hany Abdel-Latif; Mustafa Shukry; Omnia El Euony; Mohamed Mohamed Soliman; Ahmed Noreldin; Hanan Ghetas; Mahmoud Dawood; Mohamed Khallaf. 2021. "Hazardous Effects of SiO2 Nanoparticles on Liver and Kidney Functions, Histopathology Characteristics, and Transcriptomic Responses in Nile Tilapia (Oreochromis niloticus) Juveniles." Biology 10, no. 3: 183.

Journal article
Published: 01 March 2021 in Animals
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In the present study, fish were exposed to sub-lethal doses of CuONPs (68.92 ± 3.49 nm) (10 mg/L, 20 mg/L, and 50 mg/L) for a long exposure period (25 days). Compared to the control group (0.0 mg/L CuONPs), a significant dose-dependent elevation in blood urea and creatinine values, serum alanine transaminase, aspartate transaminase, and alkaline phosphatase enzyme activities were evident in CuONPs-exposed groups (p < 0.05). Fish exposure to 50 mg/L CuONPs significantly upregulated the transcription of pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1beta, interleukin 12, and interleukin 8), heat shock protein 70, apoptosis-related gene (caspase 3), and oxidative stress-related (superoxide dismutase, catalase, and glutathione peroxidase) genes in liver and gills of the exposed fish in comparison with those in the control group (p < 0.05). Moreover, varying histopathological injuries were noticed in the hepatopancreatic tissues, posterior kidneys, and gills of fish groups correlated to the tested exposure dose of CuONPs. In summary, our results provide new insights and helpful information for better understanding the mechanisms of CuONPs toxicity in Nile tilapia at hematological, molecular levels, and tissue levels.

ACS Style

Hany Abdel-Latif; Mahmoud Dawood; Samy Mahmoud; Mustafa Shukry; Ahmed Noreldin; Hanan Ghetas; Mohamed Khallaf. Copper Oxide Nanoparticles Alter Serum Biochemical Indices, Induce Histopathological Alterations, and Modulate Transcription of Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus. Animals 2021, 11, 652 .

AMA Style

Hany Abdel-Latif, Mahmoud Dawood, Samy Mahmoud, Mustafa Shukry, Ahmed Noreldin, Hanan Ghetas, Mohamed Khallaf. Copper Oxide Nanoparticles Alter Serum Biochemical Indices, Induce Histopathological Alterations, and Modulate Transcription of Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus. Animals. 2021; 11 (3):652.

Chicago/Turabian Style

Hany Abdel-Latif; Mahmoud Dawood; Samy Mahmoud; Mustafa Shukry; Ahmed Noreldin; Hanan Ghetas; Mohamed Khallaf. 2021. "Copper Oxide Nanoparticles Alter Serum Biochemical Indices, Induce Histopathological Alterations, and Modulate Transcription of Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus." Animals 11, no. 3: 652.

Journal article
Published: 23 February 2021 in Agriculture
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This study evaluates low protein diets with amino acid supplement on growth, biochemical markers and muscle amino acids profile in broilers under high ambient temperature. A total of 480 one-day-old chicks were allocated into three treatments with four replicates (n = 40). Control fed optimal protein and optimal amino acids which contains 23% and 21% crude protein (CP) with 65% methionine + cysteine/ lysine (Met + Cys/Lys) and 55% threonine/lysine (Thr/Lys), LPOA (low protein and optimal amino acids) which contains 21% and 19% CP with 65% Met + Cys/Lys and 55% Thr/Lys and 3. LPHA (low protein and high amino acids) which contains 21 and 19% CP with 74% Met + Cys/Lys and 67% Thr/Lys, respectively during the summer months (The temperatures 32 ± 3 °C, and the relative humidity 72.5% ± 4). Birds fed LPOA diets significantly highest body weight, while those fed LPHA recorded significantly the lowest body weight (BW). Dressing percentages not revealed significantly affected by reducing dietary protein levels, while the blood plasma total protein, albumin, and globulin were not significant differences due to dietary low protein. Chicks fed LPHA diets recorded the highest liver content of malonaldehyde. It could be concluded that feeding the Cobb 500 broilers on low protein diets with the same amino acid levels had no adverse effect on growth, carcass markers, and liver function, however increased amino acids levels to low protein diets may led negative impacts for the broiler performance under high ambient temperature.

ACS Style

Ahmed Saleh; Khairy Amber; Mohamed Soliman; Mahmoud Soliman; Wael Morsy; Mustafa Shukry; Mohammed Alzawqari. Effect of Low Protein Diets with Amino Acids Supplementation on Growth Performance, Carcass Traits, Blood Parameters and Muscle Amino Acids Profile in Broiler Chickens under High Ambient Temperature. Agriculture 2021, 11, 185 .

AMA Style

Ahmed Saleh, Khairy Amber, Mohamed Soliman, Mahmoud Soliman, Wael Morsy, Mustafa Shukry, Mohammed Alzawqari. Effect of Low Protein Diets with Amino Acids Supplementation on Growth Performance, Carcass Traits, Blood Parameters and Muscle Amino Acids Profile in Broiler Chickens under High Ambient Temperature. Agriculture. 2021; 11 (2):185.

Chicago/Turabian Style

Ahmed Saleh; Khairy Amber; Mohamed Soliman; Mahmoud Soliman; Wael Morsy; Mustafa Shukry; Mohammed Alzawqari. 2021. "Effect of Low Protein Diets with Amino Acids Supplementation on Growth Performance, Carcass Traits, Blood Parameters and Muscle Amino Acids Profile in Broiler Chickens under High Ambient Temperature." Agriculture 11, no. 2: 185.

Journal article
Published: 19 February 2021 in Animals
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Catalytic and physicochemical properties of microbial phytase sources may differ, affecting phosphorus (P) release and subsequently the productive and reproductive performance of layers. The current study aimed to evaluate the impact of bacterial and fungal phytase sources on layer productivity, egg production, biochemical blood indices, and reproductive morphology. For this purpose, 360 Bovans brown hens at 42 weeks of age were randomly allocated into 4 experimental groups, each with 15 replicates of 6 hens. The first group (control) was fed a basal diet with 4.6 g/kg available P. In contrast, the second, third, and fourth groups were fed diets treated with 3.2 g/kg available P, supplemented with either 5000 FTU/kg of bacterial E. coli (QuantumTM Blue 5G), fungal Aspergillus niger (VemoZyme® F 5000 Naturally Thermostable Phytase (NTP)), or fungal Trichodermareesei (Yemzim® FZ100). Dietary supplementation of bacterial and fungal phytases did not affect the productive performance or egg quality criteria, except for increased shell weight and thickness (p < 0.05). Serum hepatic function biomarkers and lipid profiles were not altered in treated hens, while calcium and P levels were increased (p < 0.05) related to the controls. Ovary index and length, and relative weight of oviduct and its segments were not influenced. The contents of cholesterol and malondialdehyde in the yolks from treated birds were lower compared to control hens, while calcium and P content increased (p < 0.05). Conclusively, bacterial and fungal phytase sources can compensate for the reduction of available P in layers’ diets and enhance shell and yolk quality without affecting productive performance, and no differences among them were noticed.

ACS Style

Ahmed A. Saleh; Mohammed Elsawee; Mohamed M. Soliman; Reyad Y. N. Elkon; Mohammed H. Alzawqari; Mustafa Shukry; Abdel-Moneim Eid Abdel-Moneim; Hatem Eltahan. Effect of Bacterial or Fungal Phytase Supplementation on the Performance, Egg Quality, Plasma Biochemical Parameters, and Reproductive Morphology of Laying Hens. Animals 2021, 11, 540 .

AMA Style

Ahmed A. Saleh, Mohammed Elsawee, Mohamed M. Soliman, Reyad Y. N. Elkon, Mohammed H. Alzawqari, Mustafa Shukry, Abdel-Moneim Eid Abdel-Moneim, Hatem Eltahan. Effect of Bacterial or Fungal Phytase Supplementation on the Performance, Egg Quality, Plasma Biochemical Parameters, and Reproductive Morphology of Laying Hens. Animals. 2021; 11 (2):540.

Chicago/Turabian Style

Ahmed A. Saleh; Mohammed Elsawee; Mohamed M. Soliman; Reyad Y. N. Elkon; Mohammed H. Alzawqari; Mustafa Shukry; Abdel-Moneim Eid Abdel-Moneim; Hatem Eltahan. 2021. "Effect of Bacterial or Fungal Phytase Supplementation on the Performance, Egg Quality, Plasma Biochemical Parameters, and Reproductive Morphology of Laying Hens." Animals 11, no. 2: 540.

Research article
Published: 09 February 2021 in Biological Trace Element Research
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The widespread industrial use of nitrite in preservatives, colorants, and manufacturing rubber products and dyes increases the possibilities of organ toxicity. Lithium borate (LB) is known as an antioxidant and an oxidative stress reliever. Therefore, this study is aimed at examining the effect of LB on nitrite-induced hepatorenal dysfunction. Twenty-eight male Swiss mice were divided into four equal groups. Group 1, the control group, received saline. Group 2 received LB orally for 5 consecutive days at a dose of 15 mg/kg bw. Group 3, the nitrite group, received sodium nitrite (NaNO2) on Day 5 (60 mg/kg bw intraperitoneally). Group 4, the protective group (LB + NaNO2 group), received LB for 5 days and then a single dose of NaNO2 intraperitoneally on Day 5, the same as in Groups 2 and 3, respectively. Samples of blood and kidney were taken for serum analysis of hepatorenal biomarkers, levels of antioxidants and cytokines, and the expression of genes associated with oxidative stress and inflammation. NaNO2 intoxication increased markers of liver and kidney functions yet decreased reduced glutathione (GSH), superoxide dismutase (SOD), and catalase activities in blood. NaNO2 also increased the expression of tumor necrosis factor (TNF-α), interleukin-1β and interleukin-6 (IL-1β and IL-6). Pre-administration of LB protected mice from oxidative stress, lipid peroxidation, and the decrease in antioxidant enzyme activity. Moreover, LB protected mice from cytokine changes, which remained within normal levels. LB ameliorated the changes induced by NaNO2 on the mRNA of nuclear factor erythroid 2-related factor 2 (Nfr2), heme oxygenase-1 (HO-1), nuclear factor-kappa B (NF-κB), transforming growth factor-beta 2 (TGF-β2), and glutathione-S-transferase (GST) as determined using quantitative real-time PCR (qRT-PCR). These results collectively demonstrate that LB ameliorated NaNO2-induced oxidative stress by controlling the oxidative stress biomarkers and the oxidant/antioxidant state through the involvement of the Nrf2/HO-1 and NF-κB signaling pathways.

ACS Style

Mohamed Mohamed Soliman; Adil Aldhahrani; Samir Ahmed Elshazly; Mustafa Shukry; Tarek Kamal Abouzed. Borate Ameliorates Sodium Nitrite-Induced Oxidative Stress Through Regulation of Oxidant/Antioxidant Status: Involvement of the Nrf2/HO-1 and NF-κB Pathways. Biological Trace Element Research 2021, 1 -9.

AMA Style

Mohamed Mohamed Soliman, Adil Aldhahrani, Samir Ahmed Elshazly, Mustafa Shukry, Tarek Kamal Abouzed. Borate Ameliorates Sodium Nitrite-Induced Oxidative Stress Through Regulation of Oxidant/Antioxidant Status: Involvement of the Nrf2/HO-1 and NF-κB Pathways. Biological Trace Element Research. 2021; ():1-9.

Chicago/Turabian Style

Mohamed Mohamed Soliman; Adil Aldhahrani; Samir Ahmed Elshazly; Mustafa Shukry; Tarek Kamal Abouzed. 2021. "Borate Ameliorates Sodium Nitrite-Induced Oxidative Stress Through Regulation of Oxidant/Antioxidant Status: Involvement of the Nrf2/HO-1 and NF-κB Pathways." Biological Trace Element Research , no. : 1-9.

Journal article
Published: 05 February 2021 in Gut Pathogens
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Shiga toxin-producing Escherichia coli (STEC) is a pathotype of E. coli that causes enteric and systemic diseases ranging from diarrhoea to severe hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). The emergence of multidrug-resistant (MDR) STEC from cattle sources has increased public health risk and limited treatment options. The prevalence of STEC was investigated in 200 raw food samples (milk and beef samples) and 200 diarrheic samples (cattle and human samples) in a matched region. The presence of stx genes (stx1 and stx2), carbapenemase-encoding genes (bla VIM, bla NDM-1, and bla IMP), and extended-spectrum β-lactamase (ESBL)-encoding genes (bla TEM group, bla CTX-M1 group, and bla OXA-1 group) was screened by polymerase chain reaction (PCR). Antibiogram and Enterobacterial repetitive intergenic consensus (ERIC)-PCR were also conducted. STEC isolates were identified in 6.5% (13/200) of food samples [6% (6/100) of milk and 7% (7/100) of beef samples] and in 11% (22/200) of diarrheic cases [12% (12/100) of cattle and 10% (10/100) of human samples]. We found that O26 (4.5%, 18/400) and O111 (1.5%, 6/400) were the most prevalent STEC serovars and were found more commonly in diarrheic samples. STEC strains with both stx genes, stx2 only, and stx1 only genotypes were present in 62.9% (22/35), 20% (7/35), and 17.1% (6/35) of isolates, respectively. Carbapenemase-producing STEC (CP STEC) isolates were found in 1.8% (7/400) of samples [0.5% (1/200) of foods and 3% (6/200) of diarrheic cases]. The bla VIM gene was detected in all CP STEC isolates, and one human isolate carried the bla NDM-1 gene. ESBL-producing STEC strains were detected in 4.3% (17/400) of samples [1.5% (3/200) of food samples and 7% (14/200) of diarrheic cases]. The bla TEM, bla CTX-M1, and bla OXA-1 genes were detected in 42.9% (15/35), 28.6% (10/35), and 2.9% (1/35) of STEC isolates, respectively. Approximately half (51.4%, 18/35) of STEC isolates were MDR STEC; all CP STEC and ESBL-producing STEC were also MDR STEC. The highest antimicrobial resistance rates were found against nalidixic acid (51.4%) and ampicillin (48.6%), whereas the lowest rates were reported against gentamicin (5.7%) and ciprofloxacin (11.4%). MDR STEC strains were 5.3 times more likely to be found in diarrheic cases than in foods (P = 0.009, 95% CI 1.5–18.7). ERIC-PCR was used for genotyping STEC isolates into 27 different ERIC-types (ETs) with a discrimination index of 0.979. Five ETs showed clusters of 2–4 identical isolates that shared the same virulence and antibiotic resistance genetic profile. Human isolates matched food isolates in two of these ET clusters (the O26 CP STEC cluster and the O111 STEC cluster), highlighting the potential cross-species zoonotic transmission of these pathogens and/or their genes in the study region. This is the first detection of CP STEC in milk and diarrheic cattle in Egypt.

ACS Style

Walid Elmonir; Samar Shalaan; Amin Tahoun; Samy F. Mahmoud; Etab M. Abo Remela; Radwa Eissa; Hanem El-Sharkawy; Mustafa Shukry; Rasha N. Zahran. Prevalence, antimicrobial resistance, and genotyping of Shiga toxin-producing Escherichia coli in foods of cattle origin, diarrheic cattle, and diarrheic humans in Egypt. Gut Pathogens 2021, 13, 1 -11.

AMA Style

Walid Elmonir, Samar Shalaan, Amin Tahoun, Samy F. Mahmoud, Etab M. Abo Remela, Radwa Eissa, Hanem El-Sharkawy, Mustafa Shukry, Rasha N. Zahran. Prevalence, antimicrobial resistance, and genotyping of Shiga toxin-producing Escherichia coli in foods of cattle origin, diarrheic cattle, and diarrheic humans in Egypt. Gut Pathogens. 2021; 13 (1):1-11.

Chicago/Turabian Style

Walid Elmonir; Samar Shalaan; Amin Tahoun; Samy F. Mahmoud; Etab M. Abo Remela; Radwa Eissa; Hanem El-Sharkawy; Mustafa Shukry; Rasha N. Zahran. 2021. "Prevalence, antimicrobial resistance, and genotyping of Shiga toxin-producing Escherichia coli in foods of cattle origin, diarrheic cattle, and diarrheic humans in Egypt." Gut Pathogens 13, no. 1: 1-11.

Preprint
Published: 25 January 2021
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In the present study, fish were exposed to sub-lethal doses of CuONPs (68.92 ± 3.49 nm) (10, 20, and 50 mg/L) for a long exposure period (25 days). Compared to the control group (0.0 mg/L CuONPs), a significant dose-dependent elevation in blood urea and creatinine values, serum alanine transaminase, aspartate transaminase, and alkaline phosphatase enzyme activities were evident in CuONPs-exposed groups (P < 0.05). Fish exposure to 50 mg/L CuONPs significantly upregulated the transcription of pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1beta, interleukin 12, and interleukin 8), heat shock protein 70, apoptosis-related gene (caspase 3), and oxidative stress-related (superoxide dismutase, catalase, and glutathione peroxidase) genes in liver and gills of the exposed fish in comparison with those in the control group (P < 0.05). Moreover, varying histopathological injuries were noticed in the hepatopancreatic tissues, posterior kidneys, and gills of fish groups correlated to the examined exposure dose of CuONPs. In summary, our results provide new insights and helpful information for better understanding the mechanisms of CuONPs toxicity in Nile tilapia at hematological, molecular levels, and tissue levels.

ACS Style

Hany M.R. Abdel-Latif; Mahmoud A.O. Dawood; Samy F. Mahmoud; Mustafa Shukry; Ahmed E. Noreldin; Hanan A. Ghetas; Mohamed A. Khallaf. Copper Oxide Nanoparticles Impair Serum Biochemical Indices, Induce Histopathological Effects, and Modulate Transcription of Pro-Inflammatory Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus. 2021, 1 .

AMA Style

Hany M.R. Abdel-Latif, Mahmoud A.O. Dawood, Samy F. Mahmoud, Mustafa Shukry, Ahmed E. Noreldin, Hanan A. Ghetas, Mohamed A. Khallaf. Copper Oxide Nanoparticles Impair Serum Biochemical Indices, Induce Histopathological Effects, and Modulate Transcription of Pro-Inflammatory Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus. . 2021; ():1.

Chicago/Turabian Style

Hany M.R. Abdel-Latif; Mahmoud A.O. Dawood; Samy F. Mahmoud; Mustafa Shukry; Ahmed E. Noreldin; Hanan A. Ghetas; Mohamed A. Khallaf. 2021. "Copper Oxide Nanoparticles Impair Serum Biochemical Indices, Induce Histopathological Effects, and Modulate Transcription of Pro-Inflammatory Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus." , no. : 1.

Journal article
Published: 05 January 2021 in Animals
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The present study evaluated the effect of three feeding methods (dry feed, wet feed or wet feed fermented with Bacillus licheniformis) on the growth performance, intestinal histomorphometry and gene expression of the lipid metabolism- and growth-related genes of broiler chickens. A total of 360 one-day-old Cobb-500 broiler chicks were randomly allotted into three groups containing four replicates with 30 birds each. The first group (control) was fed a dry mash basal diet. The second and third groups were fed wet feed and fermented wet feed. The final body weight and weight gain were reduced (p < 0.01) in the wet feed group, while they did not differ between the fermented wet feed and dry feed groups. Feed intake was not altered, and feeding on wet feed significantly (p < 0.01) increased the feed-to-gain ratio compared to the remaining groups. No differences between the three feeding methods in carcass characteristics, blood biochemistry and nutrient digestibility were observed except for crude protein digestibility, which was increased (p < 0.01) in the fermented wet feed group. Duodenal and ileal villi heights were elevated in birds fed fermented wet feeds, while crypt depth was not altered. The expression fold of IGF-1, GH and m-TOR genes in the pectoral muscle of birds fed wet feed was decreased (p < 0.05), while myostatin gene expression was elevated. Feeding on wet feed reduced the hepatic gene expression of PPARγ and increased that of FAS. In conclusion, wet feed negatively affected the broiler chickens’ efficiency under heat stress; however, fermenting the wet feed with Bacillus licheniformis improved feed utilization and birds’ performance compared to the dry feed group.

ACS Style

Ahmed A. Saleh; Mustafa Shukry; Foad Farrag; Mohamed M. Soliman; Abdel-Moneim Eid Abdel-Moneim. Effect of Feeding Wet Feed or Wet Feed Fermented by Bacillus licheniformis on Growth Performance, Histopathology and Growth and Lipid Metabolism Marker Genes in Broiler Chickens. Animals 2021, 11, 83 .

AMA Style

Ahmed A. Saleh, Mustafa Shukry, Foad Farrag, Mohamed M. Soliman, Abdel-Moneim Eid Abdel-Moneim. Effect of Feeding Wet Feed or Wet Feed Fermented by Bacillus licheniformis on Growth Performance, Histopathology and Growth and Lipid Metabolism Marker Genes in Broiler Chickens. Animals. 2021; 11 (1):83.

Chicago/Turabian Style

Ahmed A. Saleh; Mustafa Shukry; Foad Farrag; Mohamed M. Soliman; Abdel-Moneim Eid Abdel-Moneim. 2021. "Effect of Feeding Wet Feed or Wet Feed Fermented by Bacillus licheniformis on Growth Performance, Histopathology and Growth and Lipid Metabolism Marker Genes in Broiler Chickens." Animals 11, no. 1: 83.